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Zentrum fur Molekulare Biologie Heidleberg (ZMBH)

announces the

VII. ZMBH FORUM on Molecular Biology

Heidelberg, FRG, September 28-30,1992

CONTROL OF GENE ACTIVITYAND DIFFERENTIATION

Topics; Basic Mechanisms of TranscriptionMolecular Genetics of DevelopmentSignal Transduction and Regulated Transcription

Speakers:

D. Baltimore (New York)G. Draetta (Heidelberg)D. Duboule (Heidelberg)R. Ebright (New Brunswick)M. Green (Worcester)I. Grummt (Heidelberg)E. Hafen (Zurich)

A. Israel (Paris)G. Jiirgens (Miinchen)R. Kahmann (Berlin)R. Kornberg (San Francisco)A. Leutz (Heidelberg)R. Losick (Cambridge, USA)A. Nordheim (Hannover)

C. Nusslein-Vollhard (Tubingen)B. Roeder (New York)P. Sassone-Corsi (Strasbourg)G. Schiitz (Heidleberg)J. Smith (London)H. de The (Paris)H. Weintraub (Seattle)M. Yaniv (Paris)

The meeting will be free of charge and no registration is required.Lectures will start on September 28th at 8:45a.m. in the

Kommunikationszentrum, German Cancer Research Center (DKFZ) Heidelbergand will end on September 30th at about 1:00p.m.

Organizers: M. EilersA. Leutz

R. ParoT. Wirth

Committee: H. BujardT. Graf

I. Grummt

Supported by:Universitat Heidelberg, Deutsche Forschungsgemeinschaft,

Bundesmirusterium fiir Forschung und Technologies, and Industrial Sponsors

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Instructions to Authors

Please read these instructions carefully and follow them strictly to ensure thatthe review and publication of your paper is as efficient and quick as possible.The Editors reserve the right to return manuscripts that are not in accordancewith these instructions. Please note that all submissions should be accompaniedby a manuscript submittal form, a copy of which can be found at the back ofthese instructions.

SCOPE

Human Molecular Genetics publishes papers of broad significance in any aspectof human molecular genetics. Areas of interest include the molecular basis ofhuman genetic disease, chromosome structure and function, developmental geneticsand molecular aspects of cancer genetics. In general, mapping papers will onlybe considered if they are of functional significance for the field of molecular humangenetics. The molecular analysis of other species is also of interest where suchstudies are relevant to the understanding of the function of human genes.

REVIEW OF MANUSCRIPTS

Papers will normally be reviewed within two to three weeks of submission andwill be published within four to six weeks of acceptance. The Editors reservethe right to return immediately those papers which do not conform to the generaleditorial philosophy and standards of Human Molecular Genetics.

Original ArticlesThe emphasis of the Journal is on full research papers which may be of any lengthrequired for concise presentation and discussion of the data. Succinct and carefullyprepared papers are favoured both in terms of impact as well as readability.

Short CommunicationsShort Communications, defined as complete pieces of work which do not justifya full length paper, are welcomed and should not exceed 3 printed pages in length.

Mutation Reports

Analyses of mutations underlying human disease can be presented in a 1-2 pageshort report. The description of the disease, case and documentation of the mutationshould be given. Evidence indicating that the mutation actually causes the diseaseshould be included.

Mini Reviews

Mini Reviews are published by invitation only. However, a proposal for a MiniReview may be submitted in the form of a brief letter to the Editors at any time.The letter should state the topics and authors of the proposed review, and whythe topic is of particular interest to the field.

DNA Markers and PolymorphismsHuman DNA polymorphisms, sequence tagged sites and sequence variants willbe published and will be restricted in length to half a page. Preference will begiven to reports on new methods and/or DNA segments with a PIC value generally>0.7 unless they are biologically important loci or in a partially mapped regionof the genome. For further details on submission of these reports, please referto the end of these instructions.

PREPARATION OF MANUSCRIPTS

Three hard copies including all figures and tables should be submitted. Mini Reviewpapers and full research papers will be automatically typeset from author-suppliedword-processing disk. However, three hard copies should still be supplied. ShortCommunications, Mutation Reports and DNA Marker and Polymorphism paperswill be typeset manually and should be submitted in hard copy form. Proofs ofthese papers will be sent to the corresponding author by fax.

Full research manuscripts should be subdivided into the following sequenceof sections: Title page, Abstract, Introduction, Results, Discussion, Materialsand Methods, Acknowledgements, Abbreviations , References, Footnotes, Tables,Legends to Figures.

Short Communications and Mutation Reports should not be divided into headedsections. Present the Introduction, Results, Methods and Materials, and Discussionin a single section, no Abstract is required. The Acknowledgements and Referencesshould be presented as in full research papers.

Short communications are restricted in length to 3 printed pages and MutationReports are restricted in length to 1 - 2 printed pages. One printed page correspondsto approximately 1000 words without allowance for figures or tables.

General Format

The hard copies should be typed on A4 or American quarto (215mmx228mm).Dot matrix print or any print that is difficult to read is generally unacceptable.Please avoid the use of footnotes; use instead, and as sparingly as possible,parentheses within brackets. Underline only words or letters to appear in italics.Clearly identify unusual or handwritten symbols and Greek letters. Differentiatebetween the letter o and zero, and the letters I and 1 and the number 1. Markthe position of each figure and table in the margin.

Title pageThe title should be short, specific and informative. The first name, initial(s), andsurname of each author should be followed by his or her department, institution,city with postcode, and country. The fax and phone number of the correspondingauthor should also be provided. Any changes of address may be given in footnotes.The authors) to whom reprints should be addressed should be indicated.

AbstractThe second page of every full research manuscript must contain the Abstract,which should not exceed 250 words. The Abstract should be comprehensible toreaders before they have read the paper, and abbreviations and reference citaoonsshould be avoided. It is essential that the Abstract clearly states the biologicalimportance of the work described in the paper.

Acknowledgements

These should be included at the end of the text and not in footnotes. Personalacknowledgements should precede those of institutions and agencies.

ReferencesThese should be numbered in order of appearance in the text and must be listednumerically. The citation of Journals, books and multi-author books and sequencedata not published should conform with the following examples:

1. Huynh.T.V., Young.R.A. and Davis,R.W. (1988) In Glover.D.M. (ed.),DNA Cloning-A Practical Approach. IRL Press, Oxford, Vol.1, pp.49-78.

2. Holdsworth.M.J., Bird.C.R., Ray.J., Schuch.W and Grierson,D. (1987)Nucleic Acids Res., IS, 731-739.

3. Maniatis.T., Fritsch.E.F. and Sambrook,J. (1982) Molecular Cloning: ALaboratory Manual Cold Spring Harbor.

4. Smith.A. and White.B. (1991) Nucleic Acids Res. In Press.5. Muller,S.J. and Caradonna.S. (EMBL X52486).

References of the type Smith et al. (1989) should not be used.

Personal communications (J.Jones, personal comm.) must be authorized in writingby those involved, and unpublished data should be cited in the text as (unpublisheddata). References to manuscripts submitted, but not yet accepted should be citedin the text as (B.Jones and L.Smith, in preparation) and should not be includedin the list of references.

IllustrationsWherever possible, figures should be submitted in their desired final size, to fitthe width of a single column of text (88mm) or a double column of text (184mm).Any lettering should be approximately 2mm in height and should be in proportionto the overall dimensions of the figure. On the back of each figure, mark thenumber of the figure and the name of the first author, and also indicate the topmargin.

Photographs should be of sufficient high quality with respect to detail, contrastand fineness of grain to withstand the inevitable loss of contrast and detail inherentin the printing process. When several prints of the same figure are provided,please indicate which print should be used for reproduction in the Journal.

There is a special charge for the inclusion of colour plates. Please contact thePublisher for further details.

Line drawings should have clear and sharp lines. No additional artwork,redrawing or typesetting will be done. Therfore all labelling should be on theoriginal line drawing. Faint shading or stippling will be lost upon reproductionand should be avoided.

Figure legends should be included on the disk where appropriate and also ona separately numbered manuscript sheet. Define all symbols and abbreviationsused in the figure. Figures and legends should be intelligible without reading thetext of the manuscript.

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Abbreviations

Try to restrict the use of abbreviations to SI symbols and those recommendedby the IUPAC. Abbreviations should be defined and listed on a separate pagewith the footnotes. Standard units of measurement and chemical symbols ofelements may be used without definition within the body of the paper. Acronymsformed from phrases are unacceptable.

Conventions

In general, the Journal follows the conventions of the CBE Style Manual (Councilof Biology Editors, Bethesda, MD, 1983, 5th edn). Follow Chemical Abstractsand its indexes for chemical names. For guidance in the case of biochemicalterminology follow the recommendations issued by the IUPAC-IUB Commissionon Biochemical Nomenclature, as given Biochemical Nomenclature, publishedby the Biochemical Society, UK. Authors must follow the accepted nomenclaturefor human genes, as determined by the Nomenclature Committee of the HumanGene Mapping Workshops. See McAJpine.P. et al., Cytogenet. Cell Genet., 55,5 -76 (1990).

SUBMISSION TO THE EDITORS

Three hard copies of the manuscript including all figures and tables, disk ifappropriate, and a completed manuscript submitta) form should be submitted asfollows:

From the Americas to:The Editor, Human Molecular Genetics, Oxford University Press, PO Box Q,McLean, VA 22101, USATel: (703) 356 4301 Fax: (703) 356 4303

Address for all courier mail:The Editor, Human Molecular Genetics, Oxford University Press, 6819 Elm Street,McLean, VA 22101, USAPlease note ALL DNA Markers and Polymorphisms should be submitted toMcLean.

From the Rest of the World to:The Editor, Human Molecular Genetics, Molecular Genetics Group, Institute ofMolecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UKTel: ( + 865) 222397 Fax: ( + 865) 65493

If a customs declaration is required, it is essential that the contents be listed asOF NO COMMERCIAL VALUE. Bills for Customs or VAT will be returnedto the author.

If the manuscript is rejected, normally only the original figures are returned tothe author.Submission of a paper implies that it reports unpublished work andthat it is not under consideration for publication elsewhere. If previously publishedtables, illustrations or more than 200 words of text are to be included, then thecopyright holder's permission must be obtained. Copies of any such permissionletters should be included with the paper.

DISK SUBMISSION

To assist rapid publication, Mini Review papers and Full Research papers shouldbe submitted on disk. The computer format and word processor used must beclearly marked on the disk and on the manuscript submitta] form. It is importantto note that material submitted to the Editors in disk form must also be accompaniedby three hard copies as above.

Preparing the disk

Please present all sections of the paper in a single file. If necessary, tables canbe placed in a separate file.

Type the title, authors and affiliations in Journal style, i.e. in upper and lowercase, with bold font for the title and authors.

The text should be typed unjustified, without hyphenation (except for compoundwords) and at 1.5 line spacing.

Headings should be typed as follows: main (section) headings in bold uppercase; sub-headings in bold upper and lower case with the text continued on thenext line; sub-sub-headings in italic (or underlined) upper and lower case withthe text continued on the next line.

Indexing flags should not be included in the text.Enter only one space at the end of a sentence and after commas, semi-colons

and colons. No space should be inserted before these punctuation marks.Do not use lower case T (eD) or 'o' for 0 (zero). These may look interchangeable

but they are not since they have different electronic values.Do not include any copyright material (e.g. word processor software or operating

systems files) on the disk.

Save the text as a word processor file. If you have the facility to save the textas an ASCII file, make a copy of the file(s) in this format.

When sending the disk, ensure that it is adequately protected to avoid damageby bending or X-ray inspection.

ACCESSION NUMBERS

Authors should be aware that all new sequence information, including that whichextends a previously determined sequence already present in the database (andwhich already has an accession number) must be submitted to the Data Libraryfor a new accession number. This number must be provided before acceptanceof the manuscript.

REPRINTS

The Journal will provide 30 free reprints of full research and mini review papersto authors. Reprint order forms are sent out with notification of acceptance andmust be returned to Oxford University Press, Pinkhill house, Eynsham, OxfordOX8 1JJ, UK if addiuonal reprints are required. Late orders submitted after theJournal has been printed arc subject to increased prices.

POLICY

It is understood that by publishing a paper in Human Molecular Genetics, theauthors agree to make available to colleagues in academic (non-profit) researchany of the cells, nucleic acids, antibodies etc. that are not available from commercialsuppliers and are required to substantiate the scientific conclusions of the paper.

REVISION OF MANUSCRIPTS

When a manuscript is returned to the authors for revision prior to final acceptance,the revised version must be submitted within 1 month of the author's receipt ofthe referees' reports. Revised manuscripts returned after 1 month will be consideredas new submissions subject to re-review.

PUBLICATION

Manuscripts will usually be published within six weeks of acceptance.

PAGE CHARGES

Page charges are NOT levied.

PROOFS

In the interest of speed, no proofs will be sent to authors submitting manuscriptson disk. It is therefore of vital importance that the authors check their manuscriptscarefully before submission because any errors will be faithfully translated intothe typeset version.

For papers that are manually typeset, proofs will be sent by fax and shouldbe returned within 48 hours. Changes will be limited to the correction oftypographical errors.

DNA MARKERS: polymorphisms, sequence tagged sites andsequence variants

Only DNA polymorphisms, sequence tagged sites and sequence variants will bepublished, unless there is a special argument based upon homology or clinicalrelevance. Sequence variant reports must include sufficient information todemonstrate that the variation occurs in several tissues and/or individuals andis not confined to one biological source. Known gene sequences must be referredto by their accepted HGM Workshop name and symbol. For listing seenomenclature report in Human Gene Mapping 10, Cytogenetic Cell Genetic 51,13-66 (1989), or for the latest information, or by logging on to the GenomeDatabase (GDB), Johns Hopkins, Baltimore. If you do not already have log-onprivileges please apply to GDB by calling (301) 955 9705. Any anonymoussequence must be assigned a provisional HGM Workshop number (unless a numberhas already been assigned) by the GDB at Johns Hopkins, Baltimore at the abovetelephone number. DNA databank accession numbers are required for new DNAsequences. In addition, every probe must have a laboratory acronym by whichit is known. A copy of each accepted DNA sequence polymorphism and sequencetagged site report is sent to the GDB at Johns Hopkins, Baltimore.

It is necessary to include a sufficient description of the DNA polymorphism,sequence tagged site and sequence variant so that it can be adequately evaluatedand reproduced by other scientists in the field. In particular the following shouldbe included:DESCRIPTION, SOURCE AND METHOD (The type of variation such as RFLP,VNTR, point mutation, other, unkown, etc. The clinical or biological relevanceof the variation. The source of the reagents used including biological origin ofsequences, clones, etc. The methods used to analyze the variation such as PCR,

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sequencing, etc. The method description should include essential details such asPCR primer sequences, ampltcon length, amplification and hybridizationconditions, etc.)VARIATION (Polymorphism or variant distinction such as molecular weight,migration, sequence differences, etc. For polymorphisms, the frequency of alleles,number of independent chromosomes investigated and ethnic origin of sample.The total information content of the marker expressed as heterozygosity and/orPIC values. Independence of segregation or linkage of sequence variants, theorigin and number of independent sequences analyzed must be clearly stated.)OTHER ENZYMES TESTED AND NEGATIVE (for RFLP typepolymorphisms.)CHROMOSOMAL LOCALIZATIONMENDELIAN INHERITANCE (The number of families and meioses scored.)PROBE AVAILABILITY (See the Journal policy stating that probes must beavailable without restriction.)OTHER COMMENTS (Such as ease of digestion or repetitive sequences.)ACKNOWLEDGEMENTSPHOTOGRAPH OF AUTORADIOGRAPH OR GEL SHOWING DNAPOLYMORPHISM, SEQUENCE TAGGED SITE OR SEQUENCE VARIANTThe DNA polymorphism, sequence tagged site and sequence variant reports willbe processed by the Editor. The Editor reserves the right to decide whether thephotograph will be included in the report. Where several reports are beingsubmitted simultaneously from one centre and information such as description,source, methods, etc. is standard to all, authors are encouraged to combine theinformation in a single manuscript. In this case, the length restriction of half apage will not apply. Preference will be given to reports where information onbiologically important loci, new methods and/or markers with high heterozygosityor PIC values is provided.

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MANUSCRIPT SUBMITTAL FORM

Please fill in as much of this form as you can, it willgreatly help with the processing of your paper. Thisform must accompany your manuscript at submission.

MS No.Received:Revised:Accepted:

Manuscript detailsTitle:

First Author:

Data Bank no.

No. of pages: No. of Words:

No. of Figures: No. of Tables:

Category:

Corresponding Author:

Address:

Tel:

Fax:

Signature:

(This is on behalf of all the authors and signifies that they are in complete agreement with the contents of the paperand are prepared to abide by the policies of the journal)

Disk Enclosed: YES/NOHardware:Computer make/model

3"

Hard SectoredSingle-sided .Single density

3.5"

Soft Sectored _Double-sided _Double density

Disk to follow at revision: YES/NO

5.25"

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High density

Software (including version):Operating system:What are the files on the disk and what do they contain?

The Text:have you inserteditalic boldspecial characters by coding

Key/character usedDenoting

What special characters do you require?

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ISSN: 0964-6906 Human MolecularGenetics

Coden: HMGEE5

In recent years the amount and variety of research inhuman genetics has grown ever more rapidly. There isnow a need for a high quality forum to bring togetherthe best original research. Edited from Oxford andStanford by Kay Davies and Huntington F. Willard,Human Molecular Genetics is here to meet this need.

Publishing the best original research on all aspects ofhuman molecular genetics, within a maximum of threemonths from receipt of manuscript, Human MolecularGenetics can be delivered direct to your desk everymonth. Simply fill out a form below and send it to us atthe nearest address overleaf.

SUBSCRIPTION ORDER FORM(North and South America)

To order a subscription to volume 1, check the appropriateboxes below, complete the name and address section overleafin block capitals and mail to Oxford University Press. Personalsubscriptions are available to individuals paying by personalcheque or credit card.

1 Q Enter my subscription to volume 1 (9 issues in 1992) ofHuman Molecular Genetics at the following rate:

Full (library/institution) Q US$225Personal D US$95Subscribers in Canada please add 7% tax

Note : Orders must be accompanied by payment. The price includes handlingand postage. Subscriptions are entered on a per-volume basis only anddispatch will commence only after receipt of correct payment

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SUBSCRIPTION ORDER FORM(UK, Europe and Rest of World)

To order a subscription to volume 1, check the appropriateboxes below, complete the name and address section overleafin block capitals and mail to Oxford University Press. Personalsubscriptions are available to individuals paying by personalcheque or credit card.

1 Q Enter my subscription to volume 1 (9 issues in 1992) ofHuman Molecular Genetics at the following rate:

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3 Personal subscribers must complete thissection:'In accepting this personal subscription, I acknowledgethat the use of the journal will be restricted to me, that thecopyright restrictions printed in the journal will be honouredand that the issues will not be donated to a library'

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Nucleic Acids ResearchFeatures for 1992

• Extra low subscription rate for newpersonal subscribers

• Sequence supplement databases nowavailable on CD-ROM.

• FREE! As a special offer to launch thenew journal Human MolecularGenetics, all 1992 Nucleic AcidsResearch subscribers will receive anintroductory free subscription

Nucleic Acids Research is the leading rapidpublication journal for papers on physical,chemical and biological aspects of nucleicacids, their constituents and the proteins withwhich they interact. Only a fraction of thepapers submitted meet the criteria foracceptance: papers have to be significant,timely, and original. Full length papers covermolecular biology, enzymology, chemistry andgenome structure and mapping. Once accepted,average publication time is just 40 days.Presented in large American Quarto format,papers are typeset direct from disk throughout

Regular features include:For the Record - useful data which is notappropriate as a full paper. The decision topublish is made by an executive editor,ensuring very fast publication - approximatelyfive weeks. Suitable areas for inclusion are:sequence data; NMR assignment data; methods(useful improvements to existing methods).

Sequence Update - recent database entriesavailable on the EMBL File Server

Surveys and Summaries - brief reviews whichbring together material often not found in aformal review article.

NAR Sequence Supplement

In addition to 24 regular issues, yoursubscription to Nucleic Acids Research will, asusual, bring you the annual sequencesupplement In 1992, this will give you detailsof over 12 databases, explaining how they havebeen compiled, how they can be used, and howthey have changed from the previous year.

Databases now available on EMBL CD-ROMFor the first time, most of the databases :HpsrriHful in thp snnnipjnpnt arp availahlp on aUvOvl l(.̂ vxJ- 111 Ulv 3U L/L/l v l l 11/111 Ql v £1 V CUUlUlv v/11 d

single CD-ROM. This CD-ROM is producedin collaboration with EMBL and contains thefollowing databases:-Cameron The EMBL Nucleotide SequenceDatabaseIkemura Codon usage tabulated from theGenBank genetic sequence dataDe Wachter Compilation of small ribosomalRNA sequencesMcClelland Site specific methylation: effecton DNA modification methyltransferases andrestriction endonucleasesReddy Compilation of small RNA sequencesErdmann Compilation of 5S rRNA gene IsequencesBrownlee Haemophila B: database of pointmutationsKroeger Compilation of DNA Sequences ofEscherichia coliRoberts Restriction enzymes and theirisoschizomersSprinzl Compilation of tRNA sequences andsequences of tRNA genesBairoch PROSITE: a dictionary of sites andpatterns in proteinsBairoch The SWISS-PROT protein sequencedatabank

SUBSCRIPTION RATES FOR 1992 (VOLUME 20)24 ISSUES PLUS 1 SUPPLEMENT

Institutions: UK and Europe £625; Elsewhere US$1050Personal: UK and Europe £150; Elsewhere US$250

1st time personal subscribers: UK and Europe £95; Elsewhere US$175

For subscription details or to request a FREE sample copy, please write to:Journals Marketing Department (f), Oxford University Press,

Pinkhill House, Southfield Road, Eynsham, Oxford OX81JJ, UK

Oxford Journals

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Simple Sequence RepeatPolymorphism Markers

Simple sequence repeats (SSR)occur throughout the mammaliangenome and throughout thegenomes of most highereukaryotes.Because these repetitive elementsare found distributed throughoutthe genome and because theseelements frequently arepolymorphic between strains orindividuals, they have shown greatutility as genomic markers'2.Additionally, areas of interest canbe studied further throughpositional cloning3.

Length polymorphisms havebeen discovered in genomes whichcontain simple sequence repeats.SSR markers have been utilized inrat to localize multiple loci linkedto hypertension4. As the HumanGenome Project continues toconstruct genomic maps in humans,mice and other candidateorganisms, the number of genomicSSR markers will expand and theutility of these markers as a methodfor mapping traits determined bymultiple loci will increase.

The need to synthesize the largenumber of PCR primers required isa major obstacle to the widespreaduse of this powerful technique.Research Genetics has alongstanding commitment to anumber of groups developing thesemarkers and is committed tomaking these markers available toresearchers promptly and in aconvenient and economical form.New markers are constantly being

added and we encourage yourassistance with increasing the sizeand quality of our repository. We areworking toward becoming a centraldistributor for SSR markers and arepository of the most currentinformation for genomic mapsutilizing SSRs . Research Geneticsinvites all researchers both in plantand animal research to contact us foradditional information on how wecan help you with primer productionand/or distribution of your markers.

SSR markers are PCR+ primerswhich flank a simple sequence repeat,and are sold under the tradename ofMapPairs™ by Research Genetics.Typically, the forward primer iskinased and the PCR products areelectrophoresed on a polyacrylamidegel and autoradiographed. Allelesare scored and the information isanalyzed statistically to determinelinkage. Examples of the multipleuses for MapPairs™ includeestablishing genetic linkage forphenotypic traits determined bymultiple loci, characterization of newor existing strains, characterizationof cell lines and many others.

The price of a single MapPairs™ is$20.00 (discounts for large quantities)and includes both primers, in solutionand ready to use, with enough of eachprimer for 1000 PCR reactions usingthe popular 10 \l\ protocol4"5. Allmarkers can be ordered separately orin sets. Presently the largest singleSSR genomic map is for mouse. Themap is expected to expand to -1000markers soon. Many human markersfrom a number of groups are availableincluding 100 SSRs which can be

amplified under identical PCRconditions and which have beenmapped to specific chromosomes6.We anticipate a single rat map withover 400 SSR markers in the nearfuture.

REFERENCES

1. J.L. Weber and P.E. May. Am. J. Hum. Genet. 44,388(1989)

2. J.L Weber. Genomics 7, 524 (1990).3. 0. Botstein, R. White, M. Skolnick and R Davis.

Am. J. Hum. Genet. 32, 314 (1980).4. H.J. Jacob, K. Lindpainter, S.E. Lincoln, K. Kusumi,

R.K. Bunker. Y-P. Mao, D. Ganten, V.J Dzau.E.S. Lander, Cell67, 213 (1991).

5. W. Dietrich. H. Katz, S.E. Lincoln, H-S. Shin, J.Friedman, N Dracopoli. E.S Lander, Genetics,inpress.

6 T.J. Hudson, M. Engelstein, M.K. Lee. E. C. Ho,M J Rubenfield, C.P. Adams, D.E. Housman,N C. Dracopoli, Genomics, in press.

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Research GeneticsI-K00-533-4363

UK 0-800-89-1393FAX 1-205-536-9016

^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ P * > * tradtmarh oi Research Ct These primer* are designed and sold for use in the Polymer as* Cham Reaction {^CR^pCQe55^eo«?ed by patents owned by HofinMRoche Use ofthe PCR piocess requires a licence A license lor research mav be obtained by purchase and u<t*ol authorized re '*' ' — * •"-- "—•- — rT> "~*p oi by otherwise negotiating a license with Peikm-Eimer.

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HUMAN MOLECULAR GENETICS - Oxford Academic

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