Noninvasive assessment of left ventricular diastolic function by pulsed doppler echocardiography in...

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lACC VoL 10, NO.4 October I'.lH7:7H-42 CLINICAL STUDIES Noninvasive Assessment of Left Ventricular Diastolic Function by Pulsed Doppler Echocardiography in Patients With Hypertrophic Cardiomyopathy BARRY J. MARON, MD, FACC, PAOLOSPIRITO, MD, KIMBERLY J. GREEN, MA, YVONNE E. WESLEY, ROBERT O. BONOW, MD, FACe, JAVIER ARCE, MD Bethesda, Maryland 733 Hypertrophic cardiomyopathy is a primary myocardial disease in which symptoms may frequently result from impaired left ventricular relaxation, filling and compli- ance. In the present investigation, Doppler echocardi- ography was utilized to measure transmitral flow veloc- ity and thereby assess left ventricular diastolic performance noninvasively in a group of III patients representative of the broad clinical spectrum of hyper- trophic cardiomyopathy. In patients with hypertrophic cardiomyopathy, all Doppler indexes of diastolic relax- ation and filling differed significantly (p < 0.001) from those obtained in 86 control subjects without heart dis- ease, namely, prolongation of isovolumic relaxation (94 ± 24 versus 78 ± 12 ms) and of the early diastolic peak of flow velocity (244 ± 55 versus 220 ± 28 ms), as well as slower deceleration (3.4 ± 1.4 versus 4.9 ± 1.3 m/s 2 ) and reduced maximal flow velocity in early diastole (0.5 ± 0.2 versus 0.6 ± 0.1 m/s). As an apparent compen- sation for impaired relaxation and early diastolic filling, the atrial contribution to left ventricular filling was in- creased, as shown by increased late diastolic flow velocity (0.4 ± 0.3 versus 0.3 ± 0.1 m/s) and reduced ratio of maximal flow velocity in early diastole to that in late diastole (1.4 ± 0.8 versus 2.1 ± 0.9). Hypertrophic cardiomyopathy is a primary cardiac disease characterized by an increase in left ventricular wall thickness ( 1-7) and a diverse clinical and functional spectrum that includes patients with or without cardiac symptoms, ob- struction to left ventricular outflow or impaired diastolic function (8-1 I). From the Echocardiography Laboratory, Cardiology Branch, National Heart, Lung, and Blood Institute. National Institutes of Health, Bethesda, Maryland. Manuscript received January 20, 1987; revised manuscript received March 17, 1987, accepted April 3, 1987. Address ")r reprints: Barry J. Maron. MD. National Institutes of Health, Building 10, Room 7B-15, Bethesda, Maryland 20Xn. (0 by the American College of Cardiology The vast majority of patients with hypertrophic car- diomyopathy (91 [82%) of Ill) showed evidence of im- paired left ventricular diastolic performance, as assessed from the Doppler waveform. Abnormal Doppler dia- stolic indexes were identified with similar frequency in patients with (78%) or without (83%) left ventricular outflow obstruction, as well as in patients with (84%) or without (80%) cardiac symptoms. However, patients with nonobstruetive hypertrophic cardiomyopathy showed more severe alterations in the Doppler indexes of dia- stolic function than did patients with obstruction. Thus, abnormal diastolic performance as assessed by Doppler echocardiography was apparent in the vast ma- jority of the study patients with hypertrophic cardio- myopathy, independent of the presence or absence of cardiac symptoms or a subaortic pressure gradient. The high frequency with which diastolic abnormalities are identified in asymptomatic patients with hypertrophic cardiomyopathy suggests that impaired diastolic per- formance may be present at a time in the natural history of the disease when functional limitation is not yet evi- dent. (J Am Coil Cardiol 1987;10:733-42) Abnormalities of left ventricular relaxation, filling and compliance are particularly common in patients with hy- pertrophic cardiomyopathy and are generally considered to be responsible for symptoms of cardiac failure (1,9.12-31). However, the frequency with which left ventricular diastolic dysfunction occurs among patients within the broad clinical spectrum of hypertrophic cardiomyopathy (particularly those who are asymptomatic or mildly symptomatic) and the re- lation between diastolic dysfunction and obstruction to left ventricular outflow have not been well defined. Several recent investigations (32-39) have suggested that the assessment of transmitral flow velocity with pulsed Doppler echocardiography provides a noninvasive and c1in- 0735-IO,)7/X7/$3.50

Transcript of Noninvasive assessment of left ventricular diastolic function by pulsed doppler echocardiography in...

lACC VoL 10, NO.4October I'.lH7:7H-42

CLINICAL STUDIES

Noninvasive Assessment of Left Ventricular Diastolic Function byPulsed Doppler Echocardiography in Patients With HypertrophicCardiomyopathy

BARRY J. MARON, MD, FACC, PAOLO SPIRITO, MD, KIMBERLY J. GREEN, MA,

YVONNE E. WESLEY, ROBERT O. BONOW, MD, FACe, JAVIER ARCE, MD

Bethesda, Maryland

733

Hypertrophic cardiomyopathy is a primary myocardialdisease in which symptoms may frequently result fromimpaired left ventricular relaxation, filling and compli­ance. In the present investigation, Doppler echocardi­ography was utilized to measure transmitral flow veloc­ity and thereby assess left ventricular diastolicperformance noninvasively in a group of III patientsrepresentative of the broad clinical spectrum of hyper­trophic cardiomyopathy. In patients with hypertrophiccardiomyopathy, all Doppler indexes of diastolic relax­ation and filling differed significantly (p < 0.001) fromthose obtained in 86 control subjects without heart dis­ease, namely, prolongation of isovolumic relaxation (94± 24 versus 78 ± 12 ms) and of the early diastolic peakof flow velocity (244 ± 55 versus 220 ± 28 ms), as wellas slower deceleration (3.4 ± 1.4 versus 4.9 ± 1.3 m/s2

)

and reduced maximal flow velocity in early diastole (0.5± 0.2 versus 0.6 ± 0.1 m/s). As an apparent compen­sation for impaired relaxation and early diastolic filling,the atrial contribution to left ventricular filling was in­creased, as shown by increased late diastolic flowvelocity(0.4 ± 0.3 versus 0.3 ± 0.1 m/s) and reduced ratio ofmaximal flow velocity in early diastole to that in latediastole (1.4 ± 0.8 versus 2.1 ± 0.9).

Hypertrophic cardiomyopathy is a primary cardiac diseasecharacterized by an increase in left ventricular wall thickness(1-7) and a diverse clinical and functional spectrum thatincludes patients with or without cardiac symptoms, ob­struction to left ventricular outflow or impaired diastolicfunction (8-1 I).

From the Echocardiography Laboratory, Cardiology Branch, NationalHeart, Lung, and Blood Institute. National Institutes of Health, Bethesda,Maryland.

Manuscript received January 20, 1987; revised manuscript receivedMarch 17, 1987, accepted April 3, 1987.

Address ")r reprints: Barry J. Maron. MD. National Institutes of Health,Building 10, Room 7B-15, Bethesda, Maryland 20Xn.

(0 19~7 by the American College of Cardiology

The vast majority of patients with hypertrophic car­diomyopathy (91 [82%) of Ill) showed evidence of im­paired left ventricular diastolic performance, as assessedfrom the Doppler waveform. Abnormal Doppler dia­stolic indexes were identified with similar frequency inpatients with (78%) or without (83%) left ventricularoutflow obstruction, as well as in patients with (84%) orwithout (80%) cardiac symptoms. However, patients withnonobstruetive hypertrophic cardiomyopathy showedmore severe alterations in the Doppler indexes of dia­stolic function than did patients with obstruction.

Thus, abnormal diastolic performance as assessed byDoppler echocardiography was apparent in the vast ma­jority of the study patients with hypertrophic cardio­myopathy, independent of the presence or absence ofcardiac symptoms or a subaortic pressure gradient. Thehigh frequency with which diastolic abnormalities areidentified in asymptomatic patients with hypertrophiccardiomyopathy suggests that impaired diastolic per­formance may be present at a time in the natural historyof the disease when functional limitation is not yet evi­dent.

(J Am Coil Cardiol 1987;10:733-42)

Abnormalities of left ventricular relaxation, filling andcompliance are particularly common in patients with hy­pertrophic cardiomyopathy and are generally considered tobe responsible for symptoms of cardiac failure (1,9.12-31).However, the frequency with which left ventricular diastolicdysfunction occurs among patients within the broad clinicalspectrum of hypertrophic cardiomyopathy (particularly thosewho are asymptomatic or mildly symptomatic) and the re­lation between diastolic dysfunction and obstruction to leftventricular outflow have not been well defined.

Several recent investigations (32-39) have suggested thatthe assessment of transmitral flow velocity with pulsedDoppler echocardiography provides a noninvasive and c1in-

0735-IO,)7/X7/$3.50

734 MARON ET ALDIASTOLIC FUNCTION IN HYPERTROPHIC CARDIOMYOPATHY

JACC Vol. 10. No.4October 1987:733-42

ically useful expressionof left ventriculardiastolicperform­ance. Indexes of diastolic function obtained with this tech­nique have been shown to correlate well with thoseobtainedat cardiac catheterization (35,36) or with radionuclide an­giography (37) and also to have satisfactory intraobserverand interobserver reproducibility (37). Therefore, in thepresent investigation, we utilizedpulsed Dopplerechocardi­ography to characterize left ventricular diastolic perform­ance noninvasively in a large group of patients represen­tative of the pathophysiologic spectrum of hypertrophiccardiomyopathy.

MethodsSelection of patients. The study group comprises III

patientswithhypertrophic cardiomyopathy whowerestudiedprospectively between January 1983 and April 1986 at theNational Institutes of Health and met the following criteria:I) patients who were not taking cardioactive medications(primarily beta-adrenergic blocking or calcium channelblocking agents) or who could have the drugs safely with­drawn at least48 hours before the Dopplerstudy; 2) patientswho had technically satisfactory diastolic waveforms thatcould be obtained with Doppler echocardiography from theleft ventricular inflow area (as described in detail later); 3)patients with normal sinus rhythm and a heart rate < 90beats/min at the time of the Doppler study, ensuring thatthe early and late diastolic peaks of flow velocity wereseparated and that flow velocity returned to baseline afterthe early peak; and 4) patients with no evidenceof moderateor severe mitral regurgitation, as determined by clinicalexamination, contrast angiography or pulsed Dopplerecho­cardiography (40) in those patients who did not undergocardiac catheterization.

The diagnosis of hypertrophic cardiomyopathy was con­firmed in each patient by the echocardiographic demon­stration of a hypertrophied nondilated left ventricle in theabsence of any other cardiac or systemic disease capable ofproducing left ventricular hypertrophy (2). Significant nar­rowing of the extramuralcoronary arteries was excluded bycoronary arteriography in 58 of the study patients. Coronaryartery disease was considered unlikely in the other 53 pa­tients who did not undergo cardiac catheterization; 37 ofthese 53 patients were < 40 years old, and 15 of the 16patients who were 2:40 years of age were asymptomatic (ormildly symptomatic) or had no history of chest pain. Fourof the study patients had relatively mild systemic hyperten­sion, which itself was not considered to be of sufficientseverity to produce left ventricular hypertrophy of the mag­nitude that was present (41). The control group comprised86 subjects without clinical or echocardiographic evidenceof cardiovascular disease.

Demographic and clinical profiles. Patients with hy­pertrophic cardiomyopathy. The 111 patientsranged in agefrom 10 to 62 years (mean 34); 81 (73%) were male. Of

the III patients, 54 were asymptomatic (or had minimaltransient symptoms), 21 had mild symptoms (New YorkHeart Association functional class II) and 36 had moderateto severe symptoms (functional classes III and IV). Thelatter group includes five patients who survived a cardiacarrest. Patientsexperienceda wide range of symptoms. Themost common limiting symptoms were dyspnea or fatigue.or both, which occurred in 53 patients. Other less frequentfindings were chest pain (43 patients), presyncope (34patients) and syncope or cardiac arrest (18 patients).

Patients were also classified as having obstructive (23patients) or nonobstructive (88 patients) hypertrophic car­diomyopathy. dependingon whetheror not a left ventricularoutflow tract gradient 2:30 mm Hg was present under basalconditions (42). In 58 patients, this assessment was madeat cardiac catheterization (performed within 18 months ofthe Doppler echocardiographic study). In the other 53 pa­tients who did not undergo catheterization, the subaorticpressure gradient was estimated from the magnitude andduration of systolic anterior motion of the mitral valve onM-mode echocardiography (43.44).

Control subjects . The 86 control subjects without heartdisease ranged in age from 20 to 74 years (mean 36); 48(55%) were male. These control subjects and the patientswith hypertrophic cardiomyopathy did not differ signifi­cantly with respect to mean age or their distribution intoage subgroups(that is, :::::29, 30 to 49 and 2:50 years) (TableI) .

Doppler echocardiography. An AdvancedTechnologyLaboratory Mark 500 ultrasound system was utilized to per­form the Doppler echocardiographic studies. This instru­ment combines a pulsed Doppler flow analyzer with a 3MHz, two-dimensional wide angle (90°) mechanical sectorscanner, as previously described (37). The system has amovable cursor that allows positioning of the Doppler sam­ple volume anywhere in the two-dimensional sector plane.Doppler shift was detected only in that region occupied bythe sample volume. Left ventricular diastolic flow velocitywas measured at depths ranging from 9 to 13 cm. Doppleroutput of diastolic flow velocity was available as an audiosignal and as a spectral display. The spectral display wasdevelopedby subjectingthe Dopplersignal to electronic fastFourier transform analysis, with sampling at 5 ms intervalsand division of the signal into 128 frequency bins. Flowvelocity profiles appeared as a waveform composition ofsmall individual gray and black blocks and were displayedon a monitorsimultaneously witha lead II electrocardiogramand phonocardiogram, and recordedon a strip chart recorderat 100 mm/s.

Each subject was examined in the left lateral decubitusposition with the transducer at or slightly to the left of theapical impulse. The transducer was oriented to obtain anapical four chamber view of the heart that provided goodvisualization of the left ventricular cavity and maximal ex-

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MARON ET AL.DIASTOLIC FUNCTION IN HYPERTROPHIC CARDIOMYOPATHY

Table 1. Comparison of Age in Patients With Hypertrophic Cardiomyopathy and inControl Subjects

Patients WithHypertrophic Cardiomyopathy Control Subjects

Age Group No. of Mean No. of Mean(yr) Patients '!c Age (yr) Patients ?r Age (yr)

:529 46 41 19 :+: 5* 35 41 22 :+: 3*30 to 49 44 39 39 :+: 5 33 38 40 :+: 5

2':50 21 20 57 :+: 4 18 21 57 :+: 6All patients III 34 :+: 15 86 36 :+: 14

*Comparison achieved statistical significance (p < 0.005); none of the other comparisons were statisticallysignificant.

735

cursion of the mitral valve leaflets, The cursor line waspositioned through a plane traversing the left ventricle fromapex to mitral valve anulus. Care was taken to attain thesmallest possible angle between the presumed direction ofdiastolic blood flow and the orientation of the ultrasoundbeam (cursor); this angle was estimated to be zero or <200

in each subject. The sample volume was positioned in theinflow area of the left ventricle approximately I ern belowthe mitral anulus (Fig, I), and its position along the cursor

Figure 1. Stop-frame two-dimensional echocardiogram showinglocation of the Doppler sample volume (enclosed within the bro­ken circle) utilized to obtain leftventricular diastolic flow velocitywaveforms. Sample volume is positioned just below the mitralvalve anulus within the inflow area of the left ventricle, VSventricular septum, (Reproduced from Spirito et al. [37],)

line was adjusted until the highest early and late peaks ofdiastolic flow velocity were recorded and the graphic qualityof the Doppler waveform was optimal,

Quantitative assessment of diastolic flow velocitywaveforms. In each subject, the three recorded waveformswith the highest diastolic flow velocity and the steepestdescent of early diastolic flow velocity, as well as the bestsignal to noise ratio, were chosen for analysis, Each of thethree waveforms was individually characterized quantita­tively, and these values were averaged,

The following measurements were obtained (Fig, 2): I)the time interval from the aortic closing component of thesecond heart sound to the onset of diastolic flow velocity(ArO), a measure of duration of isovolumic relaxation(45); 2) duration of the early diastolic peak of flow velocity,defined as the time interval from its onset to the point whereflow velocity returned to baseline (D-F); 3) rate of decrease(or deceleration) of flow velocity in early diastole (EF); 4)maximal early diastolic flow velocity, measured as the heightof the early peak (E); 5) maximal late diastolic flowvelocity,measured as the height of the late peak (A), which is dueto atrial contraction; and 6) the ratio of maximal flow ve­locity in early diastole to that in late diastole (E/A), ameasure of the relative contributions of early and late dia­stolic flow velocity to left ventricular filling,

Points D. E andA on the flow velocity waveform wereidentified as the mid points of the gray-scale spectrum.Deceleration of early diastolic flow velocity was defined asthe slope of a straight line (EF slope) passing through themid portion of the gray-scale spectrum from the peak ofearly diastolic flow velocity (at point E) to point F, In thoseinstances in which the EF slope was a curved rather than astraight line. EF was constructed as the line identifying themaximal incline of the slope. Because flow velocity wave­forms may demonstrate wide spectral spread at the end ofthe descent of early flow velocity, point F was identified asthe intersection between the line of the descent of the earlydiastolic peak and the baseline.

In the individual patient analyses, a variable was con­sidered abnormal if the value exceeded the upper or lower

736 MARON ET AL.DIASTOLIC FUNCTION IN HYPERTROPHIC CARDIOMYOPATHY

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Table 2. 95% Confidence Limits for Normal Diastolic Indexes Assessed by Doppler Echocardiography in 86 Control Subjects

Doppler Indexes

Age Group No. of A,-D* D-F* EFSlopet Et A*(yr) Patients (ms) (ms) (m/s') (m/s) (m/s) E/AT

529 35 72 ± 12 218 ± 30 5.5 ± 1.2 0.69 ± 0.12 0.27 ± 007 2.7 ± 0.7(92) (269) (3.5) (0.49) (0.39) ( 1.5)

30 to 49 33 80 ± 12 220 ± 27 4.7 ± 1.1 0.62 ± 0.14 0.33 ± 0.07 2.0 ± 0.6(100) (267) (2.8) (0.38) (0.45) (1.0)

2'50 18 84 ± 12 221 ± 33 4.2 ± 1.2 0.59 ± 0.14 0.46 ± 0.13 1.2 ± 0.4(105) (278) (2.2) (0.35) (0.69) (0.5)

*Upper 95% confidence limits for this index are shown in parentheses; a value exceeding this number was considered abnormal. Mean ± standarddeviation appears above. tLower 95% confidence limits for this index are shown in parentheses; a value smaller than this number was consideredabnormal. Mean ± standard deviation appears above. A = maximal late flow velocity (due to atrial contraction); A1-D = isovolurnic relaxation; D-F= duration of early peak of flow velocity; E = maximal early diastolic flow velocity; E/A = ratio of maximal flow velocity in early diastole to maximalflow velocity in late diastole; and EF slope = rate of decrease (deceleration) of flow velocity in early diastole.

ResultsComparison of patients with hypertrophic cardio­

myopathy and control subjects (Table 3). As a group,patients with hypertrophic cardiomyopathy differed signif­icantly from normal control subjects with regard to each ofthe Doppler indexes of left ventricular diastolic function,

Echocardiography. An Advanced Technology Labo­ratory Mark 500 mechanical sector scanner was employedto obtain two-dimensional images of the heart in a numberof cross-sectional planes, using standard transducer posi­tions (4,46). M-mode echocardiograms were recorded witha dedicated Irex System II instrument equipped with a 2.25MHz transducer, or were derived from the two-dimensionalimage under direct anatomic visualization.

Statistical methods. Data were expressed as mean ±standard deviation. Group data were compared utilizing Stu­dent's t test or the chi-square test, where appropriate, Loweror upper 95% confidence limits were calculated using theappropriate lower or upper percent point of the Student's t

test distribution,

Table 3. Diastolic Indexes Assessed by DopplerEchocardiography in III Patients With HypertrophicCardiomyopathy and 86 Control Subjects

NS<0.001<0.001<0.001<0.001<0.001<0.001

p ValueControlSubjects

1,039 ± 13178 ± 12

220 ± 284.9 ± U0.6 ± 0.10.3 ± 0.12.1 ± 0.9

994 ± 19394 ± 24

244 ± 553.4 ± 1.40.5 ± 0.20.4 ± 0.31.4 ± 0.8

Patients WithHypertrophic

Cardiomyopathy

Abbreviations as in Table 2.

RR interval (rns)

ArD (rns)

D~F (rns)

EF slope (m/s')E (m/s)

A (m/s)

E/A

."

R

I I200msec

Figure 2. Nonnalleft ventriculardiastolicflowvelocitywaveformobtainedwith Dopplerechocardiography showingthe indexesmea­sured in this study. A = maximal late diastolic flow velocity,measured as the height of the late peak (which is due to atrialcontraction); Az-D = isovolumic relaxation time, defined as theinterval from aortic component of second heart sound (arrow) toonset of flow velocity waveform; D-F = duration of early peakof flow velocity, defined as interval from the onset of this peakto the time when flow velocity returns to baseline; E = maximalearly diastolic flow velocity, measured as the height of the earlypeak; E/A = ratio of the maximal early flow velocity to themaximal late (atrial) flow velocity; EF slope = rate of decrease(deceleration) of flow velocity in early diastole, calculated as theratio of EE' to E'F; F-C = period of diastasis. (Reproduced fromSpirito et al. [37].)

95% confidence limit for the corresponding age group, de­rived from the data of the 86 normal subjects (Table 2),Diastolic function in a given patient was judged to be ab­normal if one or more Doppler indexes exceeded the 95%confidence limit for the corresponding age group,

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MARON ET AL.DIASTOLIC ('UNCTION IN HYPERTROPHIC CARDIOMYOPATHY

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although considerable variation in the contour of the wave­

forms was observed among the patients with hypertrophiccardiomyopathy (Fig. 3). Patients with hypertrophic car­diomyopathy showed prolongation of isovolumic relaxation.increased duration of the early diastolic peak of flow velocityand slower deceleration of flow velocity in early diastole.

In addition. the height of the early diastolic peak was de­

creased and the height of the late (atrial) peak was increased;hence, the ratio of maximal flow velocity in early diastole10 maximal flow velocity in late diastole was decreased.Cycle length did not differ significantly between patientswith hypertrophic cardiomyopathy and control subjects.

Figure 3. Left ventricular diastolic waveforms ob­tained with pulsed Doppler echocardiography in sixpatients with hypertrophic cardiomyopathy. Panels Ato E demonstrate a van ely of abnormal waveforms.PanelF is a normal waveform shown for comparison.A, Typical abnormal waveform showing reduced max­imal flow velocity of early peak (E), slow decelerationof early diastolic flow velocity (from E to F). prolon­gation of the early peak DF. abbreviated diastasis andincreased maximal velocity of the late peak due to atrialsystole (A). B, Early peak E is virtually absent. whilethe late peak A is greatly increased; a prolonged iso­volumic relaxation period, from the aortic componentof the second heart sound (S2l to the initial flow velocityat point D is evident here. as well as in panels C andD. C, Early (E) and late (A) diastolic peaks showsimilar maximal flow velocity; deceleration of the earlypeak is slow. D, Similar to the profile in panel B, butthe early peak (E) is more pronounced. E, Similar tothe profile shown in panel A, but without a compen­satory increase in flow velocity of the late (atrial) peak(A). S, = first heart sound.

EA

A

DF

FD

,... ,/"'-. -- ..... " ~./\-..."-v../' .,.- '"S1

S152----.-;

rr:I • I I I I • I I I I • I I I I • I I I I I 1 . 1 I 11 .. 1.1 1 1 111 J.

738 MARON ET AL.DIASTOLIC FUNCTION IN HYPERTROPHIC CARDIOMYOPATHY

No . Patients

LV Diastolic----- Function -----

NoSymptoms

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Nonobstructive Obstructive Nonobstructive Obstructive Nonobstructive

Figure 4. Flow diagram showing occurrence of abnormal andnormal diastolic performance as assessed by Doppler echocardi­ography in III patients with hypertrophy cardiomyopathy with orwithout symptoms or outflow obstruction. LV = left ventricular.

Comparison of Subgroup s of Patients WithHypertrophic Cardiomyopathy (Fig, 4)

Individual patient data analyses. Of the III patientswith hypertrophic cardiomyopathy, 91 (82%) had abnorm alDoppler indexes of diastolic function. The proportion ofpatients with abnormal indexes was similar in each of theclinical subgroups into which the study group had beendivided. For example, the frequency of diastolic abnor­malities did not differ significantly between asymptomaticpatient s (43 [80%] of 54) and patients with cardiac symp­toms (48 [84%] of 57), or when asymptomatic and symp­tomatic patients with the nonobstruct ive form of hyper­trophic cardiomyopathy were compared (38 [78%] of 49versus 35 [90%] of 39). Also, no significant differenceswere evident in the frequen cy of abnormal Doppler indexe sof diastolic function in patients with or without chest pain(43 [83%] of 52 versus 46 [78%] of 59) or among patientswho had experienced syncope or cardiac arrest and thosewho had not (18 [82%] of 23 versus 75 [85%] of 88) , Thepercent of patients with abnormal diastolic indexes did notdiffer significantly between those with (18 [78%] of 23) andthose without (73 [83%] of 88) outflow obstruction .

In the I I I patients with hypertrophic cardiomyopathy,the Doppler variables most commonly abnormal were thoseof relaxation and early diastole , namely , isovolumi c relax­ation period (45 patients [41%]) and decelerat ion (39 pa­tients [35%J), maximal flow velocity (36 patients [32%])and duration of the early diastolic peak (31 patients [28%]),

Less comm only abnormal variables were maximal late di­astolic flow velocity (21 patients [19%]) and the ratio ofearly to late (atrial) maximal diastolic flow velocity (2 1patients [19%]),

Patien ts with nonobstructive hypertrophic cardio­myopathy more commonly showed prolonged isovolumicrelaxation (43 [49%] of88 patients) and slower deceleration(35 [40%) of 88 patients) and reduced peak flow velocityin early diastole (34 [39%] of 88 patients) than did patientswith obstructive hypertrophic cardiomyopathy (2 [9%) of23 patients, 4 [17%) of 23 patients and 2 [9%1of 23 patients,respecti vely) (p < 0.05 to < 0.001). Patients with obstruc­tion more commonly showed increased late diastolic flowvelocity (8 [35%] of 23 patients versus 13 [15%] of 88patients) (p < 0,05),

Group data analyses (Table 4). Symptomatic patient sshowed greater late (atrial) diastoli c flow velocity than didasymptomatic patient s (who did not diffe r from normal sub­jects). Patient s with and those without symptoms were notsignificantly different with regard to the other five Dopplerdiastolic indexes,

Patients without outflow obstruction showed more severeimpairment in the Doppler indexes of diastolic function thandid patient s with obstruction . When comp ared with patientswith outflow obstruction, patients with nonobstructive hy­pertrophic cardiomyopathy had more prolonged isovolumi crelaxation and slower deceleration and reduced peak flowvelocity in early diastole . Although patients with nonob ­structive and those with obstructive hypertrophic cardio­myopathy both showed increased maximal flow velocity inlate diastole compared with normal subjec ts, this increasewas greater in patient s with obstruction compared with pa­tients without obstruction . In patients with nonobstructive

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T able 4. Com pariso n of Doppler Diastoli c Ind exe s in Subgroup s o f II I Pati ents W ith Hy pe rt ro phic Card io m yo pathy (H CM)

Patient No. of Age A2-D D-F EF Slope E A

Group Patients (yr) (ms) (rns) (rn/s'') (mls) (mls) EtA

Obstructive HCM 23 37 ::: 18 7H ::: 23 25 l ::: 55 3.9 ::: 1.5 0 .66 ::: 0 .2 0 .5 1 ± 0 .2 1.5 ::: 0 .7

Nonobstructive HCM 88 33 =: 14 9H ± 24 242 ± 56 3.2 =: 1.3 OA9 ± 0. 1 0 .3H ± 0. 1 1.5 ± 0.8p < 0.00 1 P < 0.05 P < 0.00 1 P < 0.00 1

Asymptomatic 54 30 ::: 13 94 ::: 24 245 :t 45 3.5 ::: 1.0 0 .5 1 ::: 0 .2 0 .37 ± 0. 1 1.5 ::: 0.6Symptomatic 57 38 ::: 15 93 ::: 26 243 ::: 64 .1.3 :t 1.0 0.53 ::: 0. 2 OA5 ± 0.2 1.4 ::: 1.0

p < 0.01

Normal controls vs, 86 36 ::: 14 78 ::: 12 220 ::: 28 4.9 ± 1.3 0 .63 ± 0 . 1 0.33 ± 0. 1 2. 1 ± 0.9Obstructive HCM NS NS < 0.00 1 < 0.0 1 NS < 0.00 1 < 0.005Nonobstructive HCM NS < 0.00 1 < 0.005 < 0.00 1 < 0.00 1 < 0.02 < 0.00 1Asymptomatic < 0 .02 <0.001 < 0.00 1 < 0.00 1 < 0.00 1 NS < 0.00 1Symptomatic NS < 0.00 1 < 0.005 < 0.001 < 0.00 1 < 0.00 1 < 0.00 1

Abbreviations as in Table 2.

hypertrophic cardiomyopathy, all diastolic indexes differedsignificantly from normal values; in patients with obstruc­tion, all indexes differed from normal except isovolumicrelaxation and maximal early diastolic flow velocity. Pa­tients with and those without obstruction did not, however,differ with respect to the magnitude or extent of leftventricular hypertrophy or diastolic chamber dimension(Table 5).

Discussion

Studies (12-30) utilizing a variety of techniques haveshown that left ventricular relaxation, fill ing and compliance

are frequently impaired in patients with hypertrophic car­diomyopathy. It is generally believed that many of the symp­toms present in patients with hypertrophic cardiomyopathyaredue to diastolicdysfunction(1,9, 12- 31). However, mostof the previous studies of diastolic function in patients withhypertrophic cardiomyopathy have employed invasive tech­niques such as cardiac catheterization (l 2,14,20 ,21,26,31)or radionuclide angiography (19,22,23,28- 30), and con­sequently were largely confined to severely symptomaticpatients.

Doppler assessment of diastolic function. Pulsed Dop­pler cchocardiography has recently been utilized to assess

Table 5. Compari son of Left Ventricular Morphol ogy in 111 Patients with Obstruct ive orNonobstru ctive Hypert rophic Card io myopathy

Hypertrophic Card iomyopathy

Echocardiographic Variable

Ventricular septal thickness (mm)Posterior free wall thickness (mm)Septum-free wall thickness ratioLV transverse diastolic dimension (rnm)Left atrial transverse dimension (mm)LV wall thickness-LV cavity dimension ratiot

Morphologic type(two-dimensional echocardiographyjt

IIIIIIIV

Obstructive*

(n "" 23)

23.0 :::: 6 .912.3 :::: 4 . 11.9 =: 0.5

41.2 ::: 5.644.7 ::: 10.4

OA :t O. I

5/17 (29%)411 7 (230/c)8117 (47%)0117 ( 0%)

Nonobstructive*(n = 88)

22.0 ::: 6 .611.0 :t 2.72.1 :::: 0 .7

43.6 =: 5.743. 1 :t 8.3OA ± 0 . 1

31/77 (40%)17/77 (22%)19/77 (25%)10177 (13%)

*Each comparison failed to achieve statistical significance at p = 0 .05. t Ralio of wall thickness to chamberdimension calculated by averaging the septal and posterior wall thicknesses and dividing that value by the leftventricular diastolic dimen sion . :j: Morphologic type (4) could be assessed from two-dimensional echocardiogramin 17 of 23 patients with outflow obstruction and 77 of 88 patients without obstruction. Type I is localizedhypertroph y confined to anterior septum; type II is hypertrophy of anterior and posterior septum and not involvingfree wall; type III is diffuse hypertrop hy involving substantial portions of septum and free wall (usuallyanterolateral free wall); type IV is hypertroph y confined to those portions of the left ventricle not detected byconventional M-mode echocardiography (that is, anterolateral free wall. posterior septum and apical left ven­tricle) . LV = left ventricular.

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left ventricular diastolic function in normal subjects andpatients with heart disease (32-39). Doppler echocardio­graphic assessment of diastolic function has the advantagesof being noninvasive, easily performed and probably notadversely affected by the small size and abnormal geometrythat characterize the left ventricle in hypertrophic cardio­myopathy. Therefore, it seemed to us that Doppler echo­cardiography is a technique that is particularly applicableto the study of diastolic function in a large group of patientsrepresentative of the broad clinical spectrum of hypertrophiccardiomyopathy, including a substantial number of asymp­tomatic patients.

Diastolic function in hypertrophic cardiomyopathy.In our overall group of patients with hypertrophic cardio­myopathy, we observed a variety of alterations in the leftventricular diastolic waveform. The most typical pattern forthe group included prolongation of the isovolumic relaxationperiod and abnormalities of both the early and late diastolicphases (that is, prolongation, slowed deceleration and re­duced flow velocity of the early diastolic peak and increasedflow velocity of the late diastolic peak resulting from atrialcontraction, with a reduced ratio of the early to the latemaximal diastolic flow velocity). As a group, patients withhypertrophic cardiomyopathy differed significantly fromcontrol subjects without heart disease with respect to eachof the Doppler indexes of diastolic function assessed. How­ever, although these differences were statistically significantfor the intergroup comparison of mean values, the absolutedifferences may not always have been of sufficient mag­nitude to distinguish individual patients with hypertrophiccardiomyopathy from normal subjects.

The results of the present study also show that diastolicdysfunction, as assessed by Doppler echocardiography, isparticularly common in patients with hypertrophic cardio­myopathy whether or not symptoms are present. Over 80%of our patients had impaired isovolumic relaxation or anabnormal Doppler filling pattern. However, an unexpectedfinding of our study was that 80% of those patients withoutcardiac symptoms also had evidence of diastolic dysfunctiondetectable by Doppler study. Although the clinical signifi­cance of this finding is unclear at this time, it may indicatethat many patients with hypertrophic cardiomyopathy havesubclinical impairment of diastolic function (under basalconditions) at a time in the natural history of their diseasewhen functional limitation is not yet evident. Alternatively,it is possible that such alterations in the Doppler waveformdo not reflect a true diastolic abnormality that will ultimatelybecome clinically relevant to that individual patient, butrather represent an intrinsic feature of the primary under­lying cardiomyopathic process in hypertrophic cardio­myopathy. Whether symptoms will subsequently becomeclinically overt in such patients remains to be determined,and longitudinal follow-up studies will be required to definethe clinical relevance and long-term significance of abnor-

mal Doppler waveforms in asymptomatic patients with hy­pertrophic cardiomyopathy.

Diastolic function in patients with and patients with­out outflow obstruction. Our data also show that mostpatients with hypertrophic cardiomyopathy and basal ob­struction to left ventricular outflow had associated abnor­malities in Doppler indexes of diastolic function; indeed,diastolic abnormalities occurred with similar frequency inpatients with and patients without outflow obstruction. Thisobservation suggests that diastolic dysfunction is a patho­physiologic feature of hypertrophic cardiomyopathy that isnot confined to those patients with the nonobstructive formof the disease, but rather may be characteristic of the overalldisease process. Hence, cardiac symptoms and functionallimitation in patients with subaortic obstruction may resultfrom the consequences of the subaortic pressure gradient(and elevated intraventricular systolic pressure), and alsofrom impaired diastolic function.

Nevertheless, the magnitude of the abnormalities in Dop­pler diastolic indexes was greater in those patients withoutoutflow obstruction than in those with obstruction. Suchdifferences in diastolic performance (as assessed with Dop­pler ultrasound) between these two groups of patients didnot appear to be caused by differences in left ventricularmorphology, including the distribution and pattern of hy­pertrophy (4). It is possible, however, that the presence ofrelatively mild mitral regurgitation, so common in patientswith outflow obstruction (47,48), may have increased leftventricular filling velocity and, conceivably, partially maskedabnormalities of the early filling phase. Our observation thatthe isovolumic relaxation period is longer in nonobstructivecompared with obstructive hypertrophic cardiomyopathy isconsistent with previous radionuclide angiographic data (28),and may reflect the differences between these subgroups ofpatients with respect to left ventricular systolic pressure andejection time (which are load-dependent determinants of leftventricular relaxation (49,50). Thus, the higher systolicpressure and greater ejection time (51-53) in patients withoutflow obstruction could contribute to a shorter durationof left ventricular relaxation.

Our observations differ substantially in several respectsfrom those of Takenaka et al. (38), who assessed mitralflow velocity by Doppler ultrasound in 17 patients withhypertrophic cardiomyopathy. These investigators reportedthat Doppler variables of diastolic function are frequentlynormal in patients with hypertrophic cardiomyopathy, par­ticularly those without evidence of obstruction. Discrep­ancies between the present study and that of Takenaka etal. (38) could be due to the fact that they studied particularlysmall subgroups of patients (11 with evidence of obstructionand only 6 without) and just 16 normal subjects for com­parison. In addition, the methodology utilized by those in­vestigators (in which Doppler assessment of diastolic func­tion was based on an analysis of only one cardiac cycle for

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741

but rather reflect in large measure intrinsic cycle to cyclevariability of the Doppler method.

We should also emphasize that left ventricular diastolicfunction is a complex phenomenon,determinedby multiplefactors including the intrinsic properties of the cardiac mus­cle, left ventricular systolic function and loading conditionsof the ventricle (49,54-56). Hence, diastolic indexes ob­tained from the Doppler flow velocity waveform cannot beconsidered a puremeasureof diastolicfunction because theymay be influenced by such variables. Therefore, althoughour Doppler echocardiographic findings show that patientswith hypertrophic cardiomyopathy usually have alterationsin relaxation and diastolic filling patterns, we cannot becertain that these alterations are solely attributable to ab­normalities in the primary intrinsic properties of cardiacmuscle itself.

Conclusions. Using Doppler echocardiography, altera­tions in isovolumic relaxation or in Doppler indexes of di­astolic filling were identified noninvasively in the vast ma­jority of the III study patients with hypertrophiccardiomyopathy whetheror not cardiac symptoms or a sub­aortic pressure gradient was present. The high prevalenceof Dopplerabnormalities in asymptomatic patientswith hy­pertrophic cardiomyopathy implies that diastolic perform­ance may be altered at a time in the natural history of thedisease when functional limitation is not yet evident.

We recognize the quality technical assistance of Judith S. Merrill. SRNand acknowledge the superb secretarial support of Imogene Surrey.

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