SOSIS-SDS PAGE

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GOGHIQIQ U]F@IH U]F^LIO NGBIOB FHGJGO NLOBGO QNQ$UGBL

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ngug~ dlobl~gj|i ag}g goghiqiq u}f~lio ngbiob fhgjgo nlobgo QNQ$UGBL

II/ HG^G] MLHGCGOB

Dgyf}i~gq dgqyg}gcg~ Ionfolqig gnghgj d|qhid( gcgo ~l~gui |ongob$|ongob ngo

ul}g~|}go ygob ml}hgc| ni Ionfolqig ~ingc dlrgkimcgo ug}g u}fn|qlo ugobgo |o~|c

dloylnigcgo ugobgo ygob jghgh( jgoyg dl}lcg ygob iobio dloago~|dcgo hgmlh jghgh ugng

u}fn|coyg cl hldmgbg ygob ml}rlogob gbg} gug ygob nichgidoyg qlmgbgi u}fn|c jghgh i~|

mlog} gngoyg/ Fhlj cg}log i~|( ~ingc gng kgdiogo mgjrg qld|g ugobgo ygob gng ni

ugqg}go gnghgj jghgh/ Nlobgo nldicigo( cfoq|dlo d|qhid qloni}ihgj ygob jg}|q dgdu|

dldihij dgog ugobgo ygob jghgh ngo ygob ~ingc/

U}fn|c ugobgo ygob ml}mgjgo mgc| ngbiob( ~l}|~gdg ngbiob aioagob qlul}~i

ugng u}fn|c qfqiq( o|nbl~( mgcqf q|hi~ |o~|c nimlngcgo qlag}g cgqg~ dg~g gugmihg jgoyg

nihijg~ ng}i mlo~|c( ~lcq~|} ngo }gqgoyg qgkg/ Gcgo ~l~gui( gngoyg uloagdu|}go ngbiob qgui

fhlj ngbiob mgmi g~g| hldgc qgui nlobgo hldgc mgmi nghgd u}fn|c dgcgogo jlrgoi mgic

u}fn|c jlrgoi qlbg} dg|u|o u}fn|c ygob ~lhgj dlobghgdi ulobfhgjgouldgogqgo

cld|obciogo dgqij ngug~ nicloghi dlhgh|i uldl}icqgggo ygob ~lhi~i ni hgmf}g~f}i|d

nlobgo dl~fnl ygob ~lug~/ Fhlj cg}log i~| |ugyg ulob|kigo cljghghgo mgjgo ugobgo ul}h|

nihgc|cgo ~l}|~gdg nghgd dlhion|obi ngo dlokgbg cl~lo~}gdgo cfoq|dlo ygob

ml}gbgdg Iqhgd/

Gngoyg cgon|obgo cfdufolo mgjgo dgcgogo ygob dlobgon|ob mgmi nghgd

mgjgo ngo u}fn|c ugobgo ngug~ niinlo~i`icgqi dlhgh|i hldgc( u}f~lio dg|u|o NOGoyg/

Nghgd ulolhi~igo cghi ioi nihgc|cgo goghiqiq gngoyg u}f~lio ngbiob mgmi dlobb|ogcgo

lhlc~}f`f}lqiq QNQ$UGBL *Qfni|d Nfnlayh Q|h`g~$Ufhyga}yhgdinl Blh Lhla~}fujf}lqiq)

nlobgo qiq~ld m|``l} niqcfo~ioy|/



III/ ^IOKG\GO U\Q^GCG

Ogdg qfqiq nigdmih ng}i mgjgqg hg~io ygi~| Qghq|q ygob ml}g}~i ‚qgh~ln’ g~g| gqio/

Nghgd cgd|q c|hiol} Ul}goaiq( qfqiq k|bg niclogh nlobgo ogdg qg|qgbl( qg|aiqql( g~g|

qg|aiqqfo/ Ygob dldm|g~ ogdg$ogdg ioi ml}mlng( kicg qg|aiqql migqgoyg ml}mlo~|c claih

ngo qlbg} qljiobbg qlmlh|d niqgo~gu jg}|q nifhgj ~l}hlmij ngj|h| nlobgo ag}g nigqgu/

Qlngobcgo( qg|aiqqfo dldihici mlo~|c ygob mlqg}( nghgd clgnggo q|ngj nigqgu( cl}iob ngo

niqgkicgo nlobgo ag}g nii}iq/

Qfqiq gnghgj qghgj qg~| u}fn|c fhgjgo ngbiob ygob qlcg}gob d|hgi ufu|hl} ni

dgqyg}gcg~/ Iq~ihgj qfqiq qloni}i ml}gqgh ng}i mgjgqg Hg~io ygi~| qghq|q ygob ml}g}~i gqio(

dl}|k|c ugng g}~igo uf~fobgo g~g| jgoa|}go ngbiob ygob nigrl~cgo nlobgo ulobbg}gdgo/

Ng}i ~lcofhfbi u}fn|cqioyg( qfqiq nim|g~ ng}i ngbiob ygob nibihiob *nijgh|qcgo)( niml}i m|dm|

hgh| nidgq|cgo clnghgd qlhfoqfob *agqiob) ml}mlo~|c m|hg~ ugokgob qidl~}iq ygob

cld|nigo nifhgj hlmij hgok|~/

Qfqiq ygob niclogh |d|doyg ~l}m|g~ ng}i ngbiob jlrgo g~g| |obbgq aioagob

ygob niml}i m|dm| nlobgo qlnici~ agdu|}go ql}lgh g~g| ~lu|ob }f~i ygob ygob nidgq|ccgo

nghgd uiug uldm|obc|q ygob ~l}m|g~ ng}i mgjgo qio~l~iq *afhhgbloalhh|hfql) g~g| |o~|c

qfqiq ~}gniqifogh nib|ogcgo |q|q mgmi g~g| cgdmiob/ Uldm|g~go qfqiq qlag}g ~}gniqifogh

migqgoyg ~l}m|g~ ng}i ngbiob ngo hldgc mgmi/ Gngu|o U}fqlq Uldm|g~go Qfqiq dlhiu|~i;

Ngbiob niuihij *ngbiob ml}hldgc ngo ~goug hldgc)( ni~idmgob( niuf~fob( nijgh|qcgo

cld|nigo niagdu|} nlobgo mgjgo hgio/ Agdu|}go ioi cld|nigo nial~gc qlq|gi

mlo~|c ygob niiobiocgo/

Uf~fobgo ngbiob nihl~gccgo ugng }|gob ygob qlq|gi |o~|c ̀ l}dlo~gqi/ Ugng q|j| =7 ‑

=> A( `l}dlo~gqi ~l}kgni qlhgdg =$1 jg}i/ Ugng q|j| =9 ‑ 1= A( `l}dlo~gqi ~l}kgni

qlhgdg 7= ‑ 70 kgd/

U}fqlq ufq~$`l}dlo~gqi |o~|c dldmlo~|c `hgpf} ngo ~lcq~|}/ Ni \QG( gng ~gjgu ufq~$

`l}dlo~g~ifo ygob ml}|ug uldgogqgo/ Qldlo~g}g ni L}fug ~ingc gng ~gjgu ufq~$

`l}dlo~g~ifo/ Uldgogqgo ml}`|obqi |o~|c dldm|o|j dic}fmig( dlobjlo~icgo

u}fqlq ̀ l}dlo~gqi( dldm|o|j dic}fmig ug~fblo/



Gqgd gdiof dl}|ugcgo |oi~ ngqg} q~}|c~|} u}f~lio/ Q|g~| gqgd gdiof η ~l}ni}i

ng}i b|b|q gdiof( b|b|q cg}mfcqih( g~fd J ngo b|b|q ] ~l}~lo~| ygob qld|goyg ~l}icg~

ugng g~fd cg}mfo η/ G~fd cg}mfo ioi niqlm|~ η cg}log ml}qlmlhgjgo nlobgo b|b|q

cg}mfcqih *gqgd)/ B|b|q ] dloyg~gcgo }go~gi qgduiob/ Ugng u}f~lio ~l}ngug~ ldug~

~iobcg~ q~}|c~|} ygob ml}mlng ygi~|; q~}|c~|} u}idl}( q~}|c~|} qlc|onl}( q~}|c~|} ~l}qil} ngo

q~}|c~|} c|g}~lol}/

Qi`g~ ìqicfcidig u}f~lio ml}mlng qg~| qgdg hgio( ~l}bgo~|ob ugng cfdufqiqi ngo

kloiq gqgd gdiof uloy|q|ooyg/ Ml}g~ dfhlc|h u}f~lio qgobg~ mlqg} qljiobbg mihg u}f~lio

nihg}|~cgo nghgd gi} gcgo dldmlo~|c q|g~| niqul}ql cfhfingh/ Dfhlc|h u}f~lio ~ingc ngug~

dlhgh|i dldm}gol qldiul}dlgmlh( ~l~gui dgqij ngug~ dloidm|hcgo ~lbgobgo ugng

dldm}gol ~l}qlm|~/ Ugng |d|doyg u}f~lio qgobg~ ulcg ~l}jgngu ulobg}|j$ulobg}|jìqic ngo {g~ cidig( qljiobbg d|ngj dlobghgdi ul}|mgjgo mlo~|c/ Ul}|mgjgo g~g|

dfniìcgqi ugng q~}|c~|} dfhlc|h u}f~lio niqlm|~ nlog~|}gqi/ Jgh$jgh ygob ngug~

dloylmgmcgo ~l}kgnioyg nlog~|}gqi gnghgj ugogq( uJ( ~lcgogo( ghi}go hiq~}ic( ngo gngoyg

mgjgo cidig qlul}~i ghafjfh *Ygqin ngo O|}qgo~i( =550)/

Nlog~|}gqi u}f~lio ngug~ nig}~icgo q|g~| ul}|mgjgo g~g| dfniìcgqi ~l}jgngu

q~}|c~|} qlc|onl}( ~l}qil} ngo c|g}~lol}dfhlc|h u}f~lio ~goug ~l}kgnioyg uldlagjgo

icg~go$icg~go cfpghlo/ Cg}log i~|( nlog~|}gqi ngug~ nig}~icgo q|g~| u}fqlq ~l}ulagjoygicg~go jyn}fblo( io~l}gcqi jin}f`fmic( icg~go bg}gd ngo ~l}m|cgoyg hiug~go g~g| ri}|

dfhlc|h u}f~lio *Riog}of(799<)/ @looldg *7990) dloklhgqcgo hlmij hgok|~( mgjrg

nlog~|}gqi u}f~lio dlhiu|~i bgobb|go ngo cl}|qgcgo ygob d|obcio ~l}kgni ugng q~}|c~|}

qlc|onl} ngo ~l}qil} u}f~lio( nidgog q~}|c~|} u}idl} u}f~lio ~l~gu qgdg ql~lhgj u}fqlq

nlog~|}gqi/ Ugng q~}|c~|} u}f~lio ~l}qil} ~l}ngug~ ldug~ kloiq io~l}gcqi ygob dldmlo~|c

icg~go ugng }go~gi qgduiob qlul}~i icg~go jyn}fblo( kldmg~go bg}gd( icg~go niq|hìnl ngo

io~l}gcqi jin}f`fmic ofo ufhg} ygob cld|obciogo dlobghgdi bgobb|go/ Nlog~|}gqi ygob

ygob |d|d ni~ld|i gnghgj u}fqlq u}lqiui~gqi ngo cfgb|hgqi u}f~lio/

Qulc~}f`f~fdl~l} u}ioqiuoyg gnghgj q|g~| g~fd gcgo dloyl}gu qiog} ugng

ugkgob blhfdmgob ~l}~lo~| ngo uloyl}gugooyg ioi ml}bgo~|ob ugng k|dhgj g~fd ygob

gng/ Qulc~}f`f~fdl~l} ioi ngug~ nib|ogco |o~|c goghiqiq c|ghi~g~i` no c|go~i~g~i`/ Goghiqiq



c|ghi~g~i` ioi ml}ngqg}cgo ugokgob blhfdmgob ygob niql}guoyg/ Cg}log ql~igu g~fd ml}mlng

uloyl}gugoyg/ Qlngobcgo goghiqg c|go~i~g~i` ml}ngqg}cgo ng}i k|dhgj g~gfd ~g| dfhlc|h

ygob gng ygob ngug~ dloyl}gu qiog}oyg/ Jgh ioi ml}ngqg}cgo ugng j|c|d Hgdml}~ Mll}/

Uldiqgjgo u}f~lio dl}|ugcgo ~gjgu ygob jg}|q nihgc|cgo |o~|c dldulhgkg}i

qi`g~ ngo `|obqi u}f~lio/ U}f~lio ngug~ niuiqgjcgo ng}i u}f~lio kloiq hgio g~g| ng}i dfhlc|h

hgio ml}ngqg}cgo |c|}go( clhg}|~go( d|g~go ngo g`ioi~gq icg~go/ Lhlc~}f`f}lqiq gnghgj q|g~|

~lcoic uldiqgjgo dfhlc|h qlhh|hl} ml}ngqg}cgo g~gq |c|}gooyg( nlobgo dlobb|ogcgo

dlngo hiq~}ic ygob nighi}cgo ugng q|~g| dlni|d ygob dlobgon|ob qgdulh ygob gcgo

niuiqgjcgo/ Clalug~go bl}gc dfhlc|h ~l}bgo~|ob ugng oiqmgj 9}gqif) d|g~go ~l}jgngu

dgqqgoyg( ql}~g ~l}bgo~|ob u|hg ugng mlo~|c dfhlc|hoyg *Y|rfof( =55<)/ Kicg agdu|}go

u}f~lio ni~ldug~cgo ninghgd dlngo hiq~}ic( u}f~lio ml}d|g~go ufqi~i` gcgo ml}bl}gcdlo|k| lhlc~}fng ml}d|g~go ufqi~i`( ql}~g u}f~lio ygob ~ingc ml}d|g~go gcgo ~iobbgh nigd

*Hljoiobl}( 79:=)/

Lhlc~}f`f}lqiq QNQ dl}|ugcgo dl~fnl |o~|c dldiqgjcgo u}f~lio ml}ngqg}cgo

mfmf~ dfhlc|h ng}i u}f~lio ~l}qlm|~/ Clml}jgqihgo lhlc~}f`f}lqiq niagugi nlobgo

ulobb|oggo dlni|d ygob dlob|}gobi g~g| dloalbgj ~l}kgnioyg cfoplcqi ngo~ingc

ml}lgcqi nlobgo qgdulh g~g| dlobjgdmg~ ul}bl}gcgooyg qlmgbgi gcimg~ ~l}kgnioyg icg~go

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nib|ogcgo/ Blh ufhigc}ihgding ngo gbg}fqg dl}|ugcgo dg~}icq uloygobbg ygob mgoygc

nigcgi |o~|c qlug}gqi u}f~lio ngo gqgd o|chl~ *Kgoqfo( 7999)/

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Ugng dl~fnl ioi nib|ogcgo QNQ ngo ml~g$dl}cgu~fl~gofh/ QNQ dl}|ugcgo

goifoia nl~l}blo~ ygob ml}qgdg nlobgo ml~g$dl}cgu~fl~gofh ngo uldgogqgo



dloylmgmcgo }|qgcoyg q~}|c~|} ~ibg nidloqi u}f~lio dlokgni cfo`ib|}gqi }gonfd afih/ Jgh

ioi niqlmgmcgo fhlj ~l}ulagjoyg icg~go niq|h`inl ygob qlhgok|~oyg ~l}ln|cqi dlokgni b|b|q$

b|b|q q|h`jin}ih/ QNQ gcgo dldmlo~|c cfduhlcq nlobgo u}f~lio ngo cfduhlcq ioi

ml}d|g~go olbg~ipl cg}log b|b|q$b|b|q goifo ng}i QNQ *Jldlq( 799:)/



IP/ GHG^ NGO MGJGO

mgjgo ygob nib|ogcgo;

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P/ AG]G CL]KG

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ni~idmgob 75 b}gd qfqiqygob niuiqgjcgo ng}i

qlhfobqfobgooyg

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dloi~

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7(< dh ngo niqidugo q|j| $=55A qgdugi goghiqg hlmij

hgok|~



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Dgqiob$dgqiob agdu|}go cld|nigo nigdmih 755 θh

Niugogqcgo q|j| 7555A qlhgdg < dloi~

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Q~gioiob nlobgo Affdgqil m}ihigo~ mh|l 5(7,

Jgqih q~gioiob nia|ai nlobgo

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Jgqihoyg ni qagool}

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PI/ JGQIH GOGHIQG

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PII/ ULDMGJGQGO

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jgqih ioi ~ingc ngug~ nikgnicgo ng~g qlmgbgi uldmlng go~g}g u}f~lio ugng qfqiq qguin go

qfqiq mgmi/



IZ/ NG@^G] U\Q^GCG

Qfh|mihi~y Mljgpif|} f` Io~lb}gh U}f~lioq Gon Bhyafujf}ioq f` Dgddghigo

L}y~j}fay~l Dldm}gol fhlj Qgpi~g Qjg}dg ngo Q/ D/ Bfcjghl/

rrr/gklmq/afdpfh277/un` / nigcqlq ugng == Nlqldml} =577

B/ ^/ Mhlac( M/ ]/ Rji~l( N/ K/ Dihhl} ngo D/ M/ Rjllhl}/ U}fn|a~ifo f` mfpiol ghujg$

hga~ghm|dio io ~jl dihc f` ~}goqbloia uibq/ j~~u;kgq/`gqq/f}bafo~lo~207=152=/

nigcqlq ugng == Nlqldml} =577/

U|}rgoiobqij( Goiob/ Inlo~iìcgqi U}f~lio Ngbiob Qgui Ngo Mgmi Nlobgo

Lhlc~}f`f}lqiq Blh Ufhigc}ihgdin$Qfni|d Nfnlqih Q|h`g~ *Qnq$

Ugbl)/j~~u;gnho/him/|ogi}/ga/inbf/uju6in4kiu~|ogi}$bnh$q1$=55<$u|}rgoiobq

0=<&rin~j4155&UJUQLQQIN4g<g<2m0a1==g7n9m7>g=1n`>=0>70ll>

j~~u;}lufqi~f}y/ium/ga/inmi~q~}lgdjgonhl7=1><02:9>09>5MGM,=5II,=5îokg

|go,=5U|q~gcgV,=5=577~jg/un`6qlw|loal4<

j~~u;q~g``/|oy/ga/inqi~lqnl`g|h~ìhlqQhinl,=5MGM,=577,=5Dic}fmifhfbi,=5Ug

obgo/un`

j~~u;rrr/̀ cd/|oniu/ga/inng~gionlz/uju6ga~ifo4>&inz4=<7

j~~u;qailoalmif~laj/ol~~gblhla~}fujf}lqiq

http://www.ajebs.com/vol7/11.pdf


http://jas.fass.org/content/76/12/3072


http://adln.lib.unair.ac.id/go.php?id=jiptunair-gdl-s3-2005-purwanings%20625&width=300&PHPSESSID=a5a57b6c322a1d9b14a23df426416ee4




http://repository.ipb.ac.id/bitstream/handle/123456789/46940/BAB%20II%20Tinjauan%20Pustaka_%202011tha.pdf?sequence=5





http://staff.uny.ac.id/sites/default/files/Slide%20BAB%2011%20Mikrobiologi%20Pangan.pdf





http://www.fkm.undip.ac.id/data/index.php?action=4&idx=251


http://sciencebiotech.net/tag/electrophoresis/












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