INFEKSI SALURAN NAPAS

DICKY BAGUS WIDHYATMOKO

25 – 26 MARET 2015DEPARTEMEN MIKROBIOLOGI KLINIK, FAKULTAS KEDOKTERANUNIVERSITAS AIRLANGGA

POKOK BAHASAN

Streptococcus spp

Corynebacterium diphteriae

Mycobacterium tuberculosis

DISEDIAKAN

Streptococcus : S. viridans

S. pneumoniae

S. pyogenes

Corynebacterium spp

M. tuberculosis

Gram Stain

Blood Agar

Chocolate Agar

Optochin Test

Bacitracin test

PAI medium

Neisser Stain (AB, C)

Ziehl-Neelsen Stain

prepared slide

Identifikasi Streptococcus spp.

Blood Agar

Chocolate Agar

Optochin Test

Bacitracin test

Zona hemolisis

Antibiotic Sensitivity Test for Identification

First step

Gram Stain

always do gram stain before continuing to others test

Catalase Test

please learn from previous laboratory practice

Next Step : Hemolysis

hemolysis

hemolysis

hemolysis

hemolysis

partial / incomplete hemolysis

reduction red blood cell hemoglobin methemoglobin in the medium surroundingthe colony.

The greenish color is caused by the presenceof biliverdin, which is a by-product of thebreakdown of hemoglobin

e.g. S. pneumoniae, S. viridans groups

hemolysis

Streptococcus viridans Streptococcus pneumoniae

hemolysis

complete or true lysis of red blood cells

clear zone, approaching the color andtransparency of the base medium, surroundsthe colony

e.g. S. pyogenes

hemolysis

Streptococcus pyogenes

clear zone surround colony

hemolysis

lack of hemolysis / non-hemolysis

e.g. Enterococcus faecalis

hemolysis

non hemolysis, Enterococcus faecalis

Hemolysis Resume

taken from : http://iws2.collin.edu/dcain/

hemolysis

how to differentiate between S. pneumoniaewith S. viridans

Using

Optochin test

Inulin Test

Bile Solubility Test

Optochin Test

Antibiotics Susceptibility Test

Optochin also known as ethyl-hydro-cupreine

To Differentiate between S. pneumoniae withS. viridans

S > 14 mm : S. pneumoniae

No zone inhibition : S. viridans

Optochin Test

taken from http://arabslab.com

S. pneumoniae Sensitive

S. viridans groups no zone inhibition

Inulin Test

To differentiate between Streptococcuspneumoniae with all others alpha-hemolyticsstreptococci

pneumoniae hydrolize inulin

Bile Solubility Test

Bile (sodium deoxycholate)

Distinguishes S. pneumoniae from all others alpha-hemolytics streptococci

Sodium deoxycholate (2% in water) will lyse the pneumococcal cell wall

Bile Solubility Test

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