vldltion Stlldy, It tha belinnin" of the pOltural. (belore tbe patient a,tu.lly Hind,), and It tvo, five and tee ..~nute' into tbe pOltur.l Itudy. A toul'n~quat t.n be ulld al a lut -ruo-rt if tbe patient'a vein eollaplel du-rin! the Itudy.

Spat ill tubel mult be llied for the blood la~plal and Ira p-rovidld by tba CSL. The Collo"inl Itepi IInut ba foUo"ed for lbl coUeetion Ind p-rOeellinl of the Ia=plell

1. A Ipecial tube preplud by AmenhoI.llil will be lupplied by ". CBL. rbil tube contliDI Et:TA &nd Illltathioni Ind mult b. refri"erlted (2-8 C) \lntil llied.

2. The p&tient Ihould -rUloliD in the lupine pOlition ill a non­Itillilulato-ry environment fo-r It :elat 30 minute. priol' to blood ,amp.lnl. The blood llllilpLe may be drawn "ith I Iyrinle. lIemo"e tbe -rubber Itopper of In Ipp-roprilte tube Ind trlnlfec 2.~-~ cc i"lll'ediat&1y. 1'111: tbe tube sently and tho1'oulhly end "l&ce on ict! i"lll'.diately.

J. CllntrifllS' the .peei""en It ~ C for ten minutes It 900 1 (lOOO rpnd. Seper.t. pl.asma frorll the cell. II quic'dy II po"ible ["ithin .. ~ mi.n.. te. of collection), A.pirlte al lIludl. plum. ;jII,

pouibl. b"t l ....oi4 the buffy COlt Ind pl&teletl. lIecentrifule pla.ma B c, ten oinutel, 900 I).

,. Transfer to I ~ ml N..n, t .. be In4 freen. Fror.en plum. i ~

Itlble fOr It lelit tbree 1l'l0nthi "ben scored in I tightly clole4 container b'lo" -20 C.

5. Label th, tub' "ith the pltient·. initi&1., identifLtlt~on

number, IS', 4&te, And time. Include the o-risinai of the Cat.cbolamin. Spaciman Klil~nl ti.t (DeC! fo-rlll 109) "ith YOlll' shipment. A copy of the form 109 sho\l14 b. unt to tbe Coo-r4inltins Cr.nter i.n your ....eeldy eIladinl. Specimen lII\1.ll be deli .... t-red 'arly in tha week on d-ry iea to:

DCC! Centrll BioChll~iltry ~b

Attn: L2H Mlyo 626-J64~

Univerlity of l'Iinn'IDtl ~olpitlt and Ctin~e

420 0I11"lre Street Minnilipoili. Minne.otl ~S455-99aO

6. Speei01enl "ill bl nnt by CaL tD:

2 pn.O. Car4lO""lseuLlr Oivilion Biothe~ical lIe.'I-rch Lab Unive-rlity of I'Ilnnelotl

February 28, 1991 Chlpter 17

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