Sri Ramachandra College of Biomedical Sciences ...

M.Sc. Biotechnology 1

SRI RAMACHANDRA UNIVERSITY (Declared Under Section 3 of the UGC Act, 1956)

Accredited by NAAC with “A” grade

Porur, Chennai – 600116

REGULATIONS & SYLLABUS for

M. Sc. BIOTECHNOLOGY DEGREE PROGRAM Under the Choice Based Credit System - 2015

June - 2016

SRI RAMACHANDRA COLLEGE OF BIOMEDICAL SCIENCE, TECHNOLOGY & RESEARCH

Sri Ramachandra College of Biomedical Sciences, Technology and Research


Introduction:

Biotechnology is defined as the use of natural or genetically modified biological entities or their products

for the benefit of all forms of life. This branch of applied science is interdisciplinary, drawing its strength

from a plethora of basic sciences such as chemistry, physics, mathematics, biochemistry, microbiology,

plant biology, animal biology, immunology, molecular biology, environmental sciences and many others.

Biotechnology is an interdisciplinary science that enables a technology which interfaces with translational

science. It is a technology that now forms the essence of advances in also most every basic science; an

avenue for application of the fundamentals of natural science. Be it advancing knowledge in biology or

applications for enabling life, biotechnology has come to be the most productive cornerstone of its

success.

Realizing the potential role of BIOTECHNOLOGY for Indian national development, a Department of

Biotechnology was initiated at the Sri Ramachandra University during October 1999. This department

offers the M Sc Biotechnology degree program since 2001. Under the excellent ambience of a health

sciences set up, this teaching and research department is dedicated to deliver a strong multidisciplinary

teaching and research oriented program in biotechnology. This program is being offered from 2015

under the Choice Based Credit System, vide, UGC Regulations dt. 5th July 2014.

Scope:

Students graduating with a biotechnology degree have a wide scope of opportunities available in the

academia or as professional scientists. Our students are much sought after for their trained skills, their

training in a hospital setting, ability to solve problems, independence in research or assignments,

adaptability to any industrial requirement including the health care and for their decision making abilities.

In essence, we seek to enhance the capabilities of our students to make them academia/ industry ready.

They find careers in R&D of Biotechnology based companies, pharmaceutical industries including

vaccine manufacturers, diagnostic labs and kit manufacturing companies; bioinformatics companies;

certification of labs and institutions, patent laws, CROs, manufacturers of instruments and novel drugs;

and health care products including tissue engineering. Careers are also carved out during their doctoral

pursuits in India and abroad with many a student joining globally elite schools.

PROGRAM SUMMARY:

1. M Sc. Biotechnology is a postgraduate Degree Program offered under the Choice Based Credit System, with a total minimum credits of 100.

2. M Sc. Biotechnology is a 2- year semester system (4 semesters) program with 2 Semesters / year offered under the Faculty of Biomedical Sciences and Technology.

3. Students will be offered core, electives and ability enhancement courses. They are encouraged to earn extra credits by completing at least two skills enhancement courses during their study period.

4. The grading system indicates a 10-point scale of evaluation of the performance of students in terms of grade point. Grade and class will be declared as per the University rules.

5. The total performance within a semester will be indicated by a Grade point Average (GPA) and the performance in the program by cumulative Grade Point Average (CGPA). Hence CGPA is the real indicator of students‟ performance. The following table summarizes the distribution of the various courses under this program.



SUMMARY SCHEME FOR CHOICE BASED CREDIT SYSTEM Category of Courses

M Sc. Biotechnology Program

SE

ME

ST

ER

CORE COURSE (18)

DISCIPLINE SPECIFIC ELECTIVE DSE (DE)

(3)

SKILLS ENHANCEMENT

COURSE (SE/ SL) (2)

GENERIC ELECTIVE

(GE) [2]

1 CT- 1 GE-1

CT- 2

CT- 3

CT- 4

CL- 1

CL- 2

II CT- 5 DE-1 SL-1 GE-2

CT- 6

CT- 7

CT- 8

CL- 3

III CT- 9 DE-2 AE -1

CT- 10

CT- 11 Environmental Science

CT- 12

CL- 4

CL- 5

IV RP- 1 DE- 3

Distribution of Total Credits

81 9 4 6

(%) of Course types

81 19



REGULATIONS FOR M. Sc. BIOTECHNOLOGY Degree

In exercise of the powers conferred by rule 12.1 (iv) of the Memorandum of Association & Rules and clause 21 of Bye-Laws of Sri Ramachandra University, Porur, Chennai-600 116, the Academic Council of the University hereby makes the following Regulations:

1. SHORT TITLE AND COMMENCEMENT These regulations shall be called “THE REGULATIONS FOR THE M.Sc. BIOTECHNOLOGY PROGRAM OF

SRI RAMACHANDRA UNIVERSITY, PORUR, CHENNAI - 600116” under the CBCS - 2015 regulations of the University. These regulations shall be deemed to have come into force from the academic year 2015 - 2016. These regulations are subject to such modifications as may be approved by the Academic Council from time to time.

The revisions in the Regulations and syllabus will be made applicable to students admitted to the I year of the course during 2015-16 (from 3

rd semester onwards) and to students to be admitted to the I year of

the course during 2016-17 and thereafter.

2. ELIGIBILITY FOR ADMISSION Candidate for admission to M Sc Biotechnology Program in Sri Ramachandra University shall be required to possess 55% pass or B+ grade in undergraduate qualifying examination. (a) He / she should have passed the qualifying examination in:

i) B. Sc in Life Sciences (Botany, Zoology, Integrated Life Science, Biochemistry, Microbiology, Biotechnology and Nutrition)

ii) B. Sc in Physical Sciences (including physics, chemistry, mathematics and computer sciences)

iii) Bachelor of Pharmacy; B. Sc (Allied Health Sciences); B. Sc (Emergency & Trauma care technology)

iv) MBBS; B.V.Sc., B.D.S, B Sc (Ag) v) BPT; B Sc (Nursing); BASLP / BSLHS vi) B. Sc (Human genetics); B. Sc (Biomedical Science) degree of Sri Ramachandra University

or of any other university recognized as equivalent thereto.

(b) A candidate shall, at the time of admission, submit to the Head of the Institution, a Certificate of Medical Fitness from an authorized Medical Officer certifying that the candidate is physically fit to undergo the academic course and does not suffer from any disability or contagious disease.

3. ELIGIBILITY CERTIFICATE Candidates other than the Sri Ramachandra University shall get eligibility certificate from the Deemed University at the time of admission.

4. REGISTRATION A candidate admitted to the course shall register his/her name with the university by submitting the application form for registration, duly filled in along with the prescribed fee, through the Head of the Institution within the stipulated time.

5. DURATION OF THE PROGRAM The duration of M. Sc. Biotechnology program shall be for 2 academic years comprising four semesters.

6. COMMENCEMENT OF THE PROGRAM

The program will commence from 1st July of every year.

Semester Month

Odd July – December

Even January - June

7. COMMENCEMENT OF THE EXAMINATIONS

There shall be two sessions of University Examinations in an academic year, viz., December and

June.



8. CUT-OFF DATES FOR ADMISSION TO THE EXAMINATION

The candidates admitted from 1st July to 31

st August of the academic year shall be registered to take their

first semester examination, after fulfillment of the regulations concerned, in the month of December of

that academic year.

9. MEDIUM OF INSTRUCTION

English shall be the medium of instruction for all subjects of study and examinations will be conducted

only in English.

10. WORKING DAYS IN AN ACADEMIC YEAR

Each semester shall consist of not less than 100 working days and each academic year shall have a total

of 200 working days.

11. ATTENDANCE REQUIRED FOR ADMISSION TO EXAMINATIONS

a. No candidate shall be permitted to appear for the University examinations, unless he/she attends the program for the prescribed period and produces the necessary certificate of attendance and satisfactory conduct from the Head of the Institution.

b. Every candidate is required to put in a minimum of 80% of attendance in both theory and practicals separately, in each subject to be eligible for admission to the examination.

c. A candidate lacking in the prescribed attendance in any subject in theory and / practical shall not be admitted to the said course (s) during the respective semester examinations [End Semester (ES)].

12. CONDONATION OF LACK OF ATTENDANCE

It shall be open to the Vice-Chancellor to grant exemption to a candidate who has failed to obtain the prescribed 80% attendance for valid reasons based on the recommendations of HOD on payment of prescribed fee and such exemptions shall not under any circumstances be granted for attendance below 70%.

13. CURRICULUM

The curriculum and the syllabus for the programme shall be as prescribed by the Academic Council of

the University and as may be modified from time to time.

14. CREDITS

The term credit is used to describe the quantum of syllabus for various programs in terms of hours of study. It indicates differential weightage given according to the contents and duration of the courses in the curriculum design.

Credits will be assigned on the basis of the lectures (L) / tutorials (T) and/or Clinical Training (CT) / laboratory work (CL) / Research Project (RP) and other forms of learning in a 15 week schedule

1. L - One credit for one hour lecture per week (1 credit = 15 hours) 2. P/ T - One credit for every two hours of laboratory or practical (1 credit = 30 hours) 3. CR - One credit for three / two hours of clinical rotation (1 credit = 45 / 30 hours) 4. RP - One credit for two hour of dissertation/ Project work per week – Max Credit 20-25 (1credit = 30 hrs)

CREDIT VALUE PER COURSE & STRUCTURE OF SYLLABUS

For PG Programs each course will be provided a structured syllabus in the following style:

Category Credits Syllabus units

Core 3 - 4 7

Core Lab 4 - 5 10- 15 experiments

Discipline Specific elective (DE) 3 6

Discipline Specific elective with Lab (DSL) 3 with 1 credit for practicals 5 with 1 unit of practicals

Ability enhancement (AE) 2 5

Skills enhancement (SE)/ (SL)- theory or practicals

2 5

Core Projects 18-25



Minimum credit allocation for a course is as per the curriculum designed by the department. For the M Sc Biotechnology Program, each course is separate and has to be passed independently even if it carries a practical component within the syllabus.

Types of Courses Courses in a programme may be of three kinds:

Core Course

Elective Course

Ability Enhancement Course

15. COURSE CODE AND NUMBERING PATTERN The course code explains the department where the course is offered and the year and semester in which it is offered. Each semester shall have Core theory/ electives/ AECC foundation courses with numbers from 01, 02 – 50 and lab courses from 51, 52- 60; and so on as shown below:

Each course will be numbered as given below:

Co

urs

e N

um

ber

Ten Digit Course Code

Category Course Credits / Week

Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical

Tra

inin

g (

CR

)

Pra

ctical (P

)/

Researc

h

Pro

ject

(RP

)

Cre

dit

s (

C)

3 PBT 15CT 103

Core Theory - 1 Microbiology 4 - - 4

8 PBT 15CL 152

Core Lab - 1 Cell biology and Microbiology

- - 4 4

15.1 The above mentioned ten digit course code defines the following:

S. No. Code Details

1. C. No. 3, 8 Denotes Course Number for Biotechnology Program

2. U/P/I/D Denote Undergraduate, Postgraduate, Integrated and Diploma courses

3. BT Denotes Unique Two Digit program specific Code - Biotechnology

4. 16 Syllabus Updated Year

5. CT, CL; DE/ DL, GE/AE, SE/SL; CR/RP

Core Theory, Core Lab, Discipline Specific Elective [DE]/ Lab [DL]; Generic Elective [GE] & Ability Enhancement Courses [Ability Enhancement Compulsory Courses [AE]; Skill enhancement Courses [SE] Theory or SL (practical); Clinical rotation / Research Project

6. 1 03 Denotes The first digit 1 – for the First Year; the remaining two digits – “03” indicate a course in the odd semester theory (and hence it is odd number; ranging from 01-20 for Core theory).

7. 1 52 Denotes The first digit 1 – for the First Year; the remaining two digits – “52” indicate that this course is in the even semester (and hence it is even number; If greater than fifty indicates lab course).

8. Microbiology Course / Subject title

9. L-T-P-C Denotes Lecture, Tutorial, Practical & Total Credits

10. 3-0-1-4 Denotes The first number indicated it has 3 lecture credits [1 hour of lecture = 1 credit], 0 tutorials credit and [practical, P- 2 hours = 1 credit] and the last number indicate the total credit / week (4).

16. GRADING SYSTEM

The term grading system indicates a 10 point scale of evaluation of the performance of students

in terms letter grade and grade points.

16.1 Letter Grades and Grade Points:

i. SRU would be following the absolute grading system, where the marks are compounded to grades based on pre-determined class intervals.



ii. The UGC recommended 10-point grading system with the following letter grades are given below:

16.2 CBCS Grading System - Marks equivalence table of Grades and Grade Points

Letter Grade Grade Point Range of Marks

O (Outstanding) 10 86-100

A+ (Excellent) 9 70-85

A (Very Good) 8 60 -69

B+ (Good) 7 55 -59

B Pass 6 50- 54

RA (Reappear) 0 49 and below

Ab (Absent) 0 -

NC- Not Completed 0 - 16.3 Computation of SGPA and CGPA The UGC recommends the following procedure to compute the Semester Grade Point Average (SGPA) and Cumulative Grade Point Average (CGPA): i. The SGPA is the ratio of sum of the product of the number of credits with the grade points scored by a student in all the courses taken by a student and the sum of the number of credits of all the courses undergone by a student, i.e., SGPA (Si) =∑(CixGi) /∑Ci where Ci is the number of credits of the ith course and Gi is the grade point scored by the student in the ith course. ii. The CGPA is also calculated in the same manner taking into account all the courses undergone by a student over all the semesters of a programme, i.e., CGPA = ∑(Ci x Si) / ∑ Ci where Si is the SGPA of the ith semester and Ci is the total number of credits in that semester. iii. The SGPA and CGPA shall be rounded off to 2 decimal points and reported in the transcripts.

Illustration of Computation of SGPA and CGPA Computation of SGPA

iv. Grade card (Format): Based on the above recommendations on Letter grades, grade points, SGPA and CGPA, the HEIs may issue the grade card for each semester and a consolidated grade card indicating the performance in all semesters.

17. EXAMINATION & ASSESSMENT In consonance with the University regulations provided in the handbook, the assessment for M Sc Biotechnology Degree courses is as follows: i. For all category of courses offered (Core Theory, Core Lab, Discipline Specific Elective [DE]/ Lab [DL]; Generic Elective [GE] & Ability Enhancement Courses [AE]; Skills enhancement Courses [SE] Theory or SL (practical) & Research Project), the assessment will comprise of Continuous Internal Assessment (CIA) and the end – semester (ES) examination. For each course the total of 100 per course is determined from the CIA evaluation weighted at 50% and the ES weighted at 50%. ii. Evaluation for a course shall be done on a continuous basis. The uniform procedures to be adopted under the CBCS is to conduct atleast three continuous internal assessments (CIA) followed by one end semester university examination (ES) for each course. iii. Evaluation for a course with clinical rotation or clinical training or industrial visit will be done on a continuous basis. iv. Candidates having ≥ 80% attendance and obaining the minimum requirement for internal assessments in each of the courses can alone qualify to appear for the end semester examinations.

Semester 1 Semester 2 Semester 3 Semester 4

Credit : 25 SGPA : 7.7




Illustration for CGPA 25 x 7.7 + 25 x 6.8 + 25 x 6.6 + 25 x 6.0

CGPA = ________________________________ = 6.8

100



17.1 CONTINUOUS INTERNAL ASSESSMENT (CIA):

CIA forms the formative assessment component of evaluation in CBCS. It is structured to elicit the students’ domain knowledge, analytical and creativity skills besides team building. The CIA for each of the courses would have a total weightage of 50 marks which would be assessed in the pattern recommended for specific postgraduate programs. The detailed schematic of the formative and summative assessments for each of the core, electives and ability enhancement courses is indicated below.

17.2 Scheme for type of Assessments for M Sc Biotechnology

Continuous Internal Assessment

Core Theory Courses/ Discipline Specific Elective Courses

Type CA 1 CA2 CA 3

Total marks (Average)

Marks 50 50 50 50

Format

Quiz/ Test/ MCQs (1)

Scientific communications (2) Assignment/ journal articles/ creation of animated teaching lessons etc. / Posters

Test/ MCQs or Practicals for Lab cum Theory Courses; short answers; essays

50

hours of exams/ test

1.0 2.0 3.0

Core Practicals

Type CA 1 CA2 CA3 Total marks

Marks 50 50 50 50

Format

Test: Spotters - MCQs; concept testing; analytical problems; monitoring the record books

Scientific communications; journal articles/ data analysis/ experimental analysis/ Projects with Bioinformatics/ monitoring the record books

test/ MCQs Practical test with record book

50

hours of exams/ test

1.00 2.00 3.00 ---

Generic Electives/ Ability Enhancement courses/ Skills enhancement Courses

Type CA 1 CA 2 CA 3 Total marks

Marks 50 50 50 50

Format Quiz/ Test/ MCQs (2) Or Case studies - Use of Reference managers; components of manuscript/ proposal/ effective writing

Scientific communications (2) Assignment/ journal articles/ creation of animated teaching lessons etc. Posters/ Presenting papers in conferences or workshops

Test/ MCQs or Practicals for Lab cum Theory Courses; short answers; essays OR Review of literature; [Submission of typed; bound document of Thesis proposal/ Pre-proposal at the end of 3

rd semester;

ethical clearances applied or obtained

50

Core Project

Type CA 1 CA 2 CA 3 Total marks

Marks 50 50 50 50

Format Scientific communications

Work Presentation Work presentation

Description Bioinformatics tools applied in the project; Oral presentation

Research Design and methodology; laboratory notebook entry using standard protocol; verified by guide

Data obtained; analysis; One paper/ presentation to be accepted or submitted; Communicating a paper will attract more marks;

hours of exams Date communicated as per circular

0.30 hours each 0.30 hrs each ---



In general, at the beginning of each semester, curriculum plan will be drawn in which the schedule for conduct of exams is announced and circulated to students by way of a time table with dates, timings and format of exams.

(a) There will be a minimum of three continuous assessments during each semester for each course.

(b) Continuous assessment will be a combination of a variety of assessment tools such as class test, assignment, paper presentation, practicals, discussions/ role plays; spotters / Posters etc. that would be suitable to the course. This requires an element of openness.

(c) The students will be informed in advance about the nature of assessment and the procedures.

(d) Tests will be conducted giving sufficient time for preparation.

(e) No repeat, reschedule and postponement of the assessment date will be permitted. Students shall compulsorily attend any three continuous assessments.

(f) Student failing to attend any of the continuous assessments of a course should reappear (RA) for these assessments in order to pass the course.

(g) In case of students who could not attend any of the continuous assessment tests due to medical reasons or any other genuine reasons, a separate test shall be conducted by the course teacher. The same has to be approved by the Head of the Department / the Dean/ Principal.

(h) Eligibility to appear for university exams at the end of the semester requires 50% in the CIA and 80% attendance throughout the semester for each course.

(i) Candidate will not be eligible to take up the examination only for the Course(s) for which they lack 80% of attendance and / or B grade (50% marks) in the CIA.

(j) Candidates reappearing (RA) for such courses in subsequent semesters shall be permitted to appear for the continuous assessments, including in the elective departments, providing an opportunity to complete the grade requirements for that program.

(k) Laboratory record books shall be considered for the award of grades in lab courses.

(l) The continuous internal assessments shall be submitted to the University by the HoD/ Principal 15 days prior to the commencement of the University examinations.

18. END SEMESTER ASSESSMENTS: There shall be End Semester examinations for 3 hours duration carrying 100 marks for each course covering the entire syllabus. It maybe in the form of written exams (EST) or laboratory based examination (ESP)/ Viva. There shall be two sessions of University Examinations in an academic year viz., June and December. The end semester examination will be conducted by the Controller of Examinations office (CoE) for duration of 3 hours. The end semester will cover all the syllabi of the course paper for 100 marks.

QUESTION PAPER PATTERN FOR UNIVERSITY EXAMINATIONS (Theory)

S. No.

Pattern & Choices Numbers Marks

A Semester University Theory Examination

1. Objective type questions = 15. Define/ one word / sums (All compulsory)

10 x 3 30

2. Short Essay questions (5 out of 7) 5 x 8 40

3. Long Essay question 2 out of 3 2 x 15 30

Total 100

B QUESTION PAPER PATTERN FOR UNIVERSITY EXAMINATIONS (Practicals)

4. Major 1/ Minor 1 or 2; Spotters (5 -10 nos); Viva; Record

All compulsory

50

Total 50

The type of assessments and the marks for each assessment is provided in the table above. Students will be encouraged to attend all classes and course work.



18.1 SUBMISSION OF PRACTICAL RECORD BOOK At the time of practical examination, each candidate shall submit to the examiners the practical workbook duly certified by the Head of the Department as a bonafide record of work done by the candidate. Separate assessments of laboratory record which provide formal training of students for careers in academic or professional research ensures importance of maintaining lab records. 18.2. DISSERTATION

(a) All candidates registered for the M Sc. Biotechnology degree shall submit a dissertation at the end of

4th semester.

(b) Each candidate will be assigned a recognized guide at the beginning of third semester.

(c) The topic assigned to the candidate will be intimated to the HoD for registration of topic within fifteen

days of the beginning of third semester. The same shall be sent to the controller of examination of this

University.

(d) Candidate maybe permitted to obtain approval by the appropriate ethics (medical and animal) committees by the end of the third semester. (e) The dissertation work will be individual dissertation and will consist of experimental work and data collection. (f) The dissertation work shall be submitted for evaluation on the date announced in a bound volume not exceeding 150 pages (double line spacing and on one side of A4 size paper) excluding references. (g) Four copies of the dissertation work shall be submitted one month prior to the commencement of the University examination and forwarded to the Controller of Examination of the University. Continuous and Summative assessments for Dissertations: a) The dissertation / core project will be taken up by each student during the 4

th semester of their

course work. b) The head of the department/ interdepartmental examiner may evaluate the performance of the

candidate for the formative assessment marks (50). The type of assessments and marks allotted is provided in table under section 17.2. A laboratory notebook/ logbook / practical observation notebook maintained by the student and endorsed by the guide will be evaluated during the final continuous assessments. The objective is to encourage students to maintain and learn how to record independently planned exercises – an international requirement. The passing minimum of “B” grade or 50% and 80% attendance is essential for appearing for end semester University examinations.

c) ESP & Viva voce: the total of 50 % is determined from the evaluation weighted at 25% for the dissertation and 25% for the viva voce (W: 25 % each) by each examiner. Dissertation shall be evaluated by two examiners (25 each) under the following scheme - Introduction and review of literature (3 marks); Objective and scope (5); Methodology (2); Results and discussion (10), Summary and citations (5). This evaluation would reflect the quality of work put into the dissertation by the student

19. GRADE QUALIFYING FOR A PASS A candidate shall be declared to have passed the examination if he/she obtains the following minimum qualifying grade / marks:-

(a) Grade B (50% of marks) in the University End Semester Examination Theory, Practical, Clinical Rotation/ training & Dissertation (ESE).

(b) Grade B (50%) in aggregate in each course which includes both Continuous Internal Assessment and End Semester Examinations.

19.1 REAPPEARANCE FOR ARREAR SUBJECTS: Students reappearing in the end semester examinations shall do so along with the subsequent regular end - of semester examinations.

20 CARRY OVER OF „RA”Grade (Reappearance) COURSE(S): a) Candidates are permitted to carry-over the „RA‟ course(s) from any semester till the completion of the program. b) The maximum period of study permissible to complete the course is four years.



21. CLASSIFICATION OF SUCCESSFUL CANDIDATES – Overall Performance in a Program and Ranking of a candidate is in accordance with the University

regulations. Consolidated Grade Card

Letter Grade Grade Point CGPA

O (Outstanding) 10 9.01 – 10

A+ (Excellent) 9 8.01 - 9.00

A (Very Good) 8 7.01 - 8.00

B+ (Good) 7 6.0 1- 7.00

B (Above Average) 6 5.01- 6.00

A successful candidate

(i) Who secures not less than O grade with a CGPA of 9.01 – 10.00 shall be declared to have secured „OUTSTANDING‟ provided he/she passes the whole examination in the FIRST ATTEMPT;

(ii) Who secures not less than A+ grade with a CGPA of 8.01 – 9.00 shall be declared to have secured „EXCELLENT‟ provided he/she passes the whole examination in the FIRST ATTEMPT;

(iii) Who secures not less than A grade with a CGPA of 7.01 –8.00 and completes the course within the stipulated course period shall be declared to have passed the examinations with “Very Good”

(iv) All other candidates (with grades B and above) shall be declared to have passed the examinations.

22. MIGRATION / TRANSFER OF CANDIDATES Application seeking Migration/Transfer of a candidate from any recognized institution to the University shall be considered subject to the condition that: (a) there must be vacancy in the particular year of study; (b) transfer shall be effective only at the beginning of an academic year; and (c) Approval of the Vice-Chancellor shall be obtained. (d) Students shall be allowed to take credits earned from other institutions and vice-versa under

CBCS as per norms of the UGC. 23. RE-ADMISSION AFTER BREAK OF STUDY

As per University rules.

24. DISCHARGE FROM THE PROGRAM If a student admitted to a Program of study in this University is for any reason not able to complete the Program or qualify for the degree by passing the examinations prescribed within a period comprising twice the duration prescribed in the Regulations for the concerned Program, he/she will be discharged from the said Program, his/her name will be taken off the rolls of the University and he/she will not be permitted to attend classes or appear for any examination conducted by the University thereafter. 25. VACATION

The Head of the Institution shall declare vacation not exceeding six weeks in an academic year.



26: Scheme of Curriculum and Examinations, 2016

M Sc Biotechnology Degree Programme – Year 1; Semester 1, 2016

Co

urs

e N

um

ber

Course Code

Cate

go

ry

Course Title RG

Credits/Week Hours/ semester (Credits x 15 weeks)

Att

endance (

%)

Co

nti

nu

ou

s I

nte

rnal

assessm

en

t [T

heo

ry /

Pra

cti

cal]

(a)

(CIA

)

End Semester Assessment

Grand Total

Lectu

re (

L)

Tuto

rial (T

)/ C

linic

al

train

ing (

CT

) P

ractical

(P)/

Researc

h

Pro

ject

Cre

dit

s (

C)

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory (b)

Practical/ Viva (c))

Theory

: a+

(b÷2)

= 1

00 P

ractical:

a +

c =

100

EST ESP

1 PBT15CT101 CT1 Biochemistry of Biomolecules 4 - - 4 60 - 60 80 50 100 - 100

2 PBT16CT103 CT2 Advanced Cell Biology 4 - - 4 60 - 60 80 50 100 - 100

3 PBT15CT105 CT3 Analytical Techniques 4 - - 4 60 - 60 80 50 100 - 100

4 PBT16CT107 CT4 Advanced Microbiology 3 - - 3 45 - 45 80 50 100 - 100

5 PBT15GE109 GE1 To be chosen 3 - - 3 45 - 45 80 50 100 - 100

6 PBT15CL151 CL1 Biomolecules & Analytical techniques (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

7 PBT16CL153 CL2 Biology of cells and microbes (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

Semester 1 Total 18 0 8 26 270 240 510

350 500 100 700



M Sc Biotechnology – Year 1, Semester-II; 2016

Co

urs

e N

um

ber

Course Code

Cate

go

ry

Course Title

Credits/Week Hours / Semester- 15 weeks

Att

endance (

%)

Co

nti

nu

ou

s In

tern

al

assessm

en

t [T

heo

ry /

P

racti

cal]

(a)

(CIA

)


Grand Total

RG Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical tr

ain

ing

(CT

)

Pra

ctical

(P)/

Researc

h

Pro

ject

Cre

dit

s ©

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory (b)

Practical/ Viva (c)

Theory

: a+

(b÷2)

= 1

00

Pra

ctical: a

+ c

=

100

EST ESP

8 PBT15CT102 CT5 Molecular Biology 4 - - 4 60 - 60 80 50 100 - 100

9 PBT15CT104 CT6 Bioprocess Technology

4 - - 4 60 - 60 80 50 100 - 100

10 PBT15CT106 CT7 Biological Calculations and Biostatistics

4 - - 4 60 - 60 80 50 100 - 100

11 PBT16CT108 CT8 Protein Biology * 3 - - 3 45 - 45 80 50 100 - 100

12 PBT15DE110 DE1 Bioinformatics [BGE023]

2 - 1 3 30 30 60 80 50 100 - 100

13 PBT15GE112 GE2 Choice – (any One) 3 - - 3 45 - 45 80 50 100 - 100

14 PBT15CL152 CL3 Protein Methods (Practicals)

- - 4 4 0 120 120 80 50 - 50 100

15 PBT15SL154 SL1 Animal Cell Culture Technology Practicals)

1 - 1 2 15 30 45 80 50 - 50 100

Semester II Total 21 0 6 27 315 180 495

400 600 100 800



M Sc Biotechnology – Year 2 Semester-III, 2016

Co

urs

e N

um

ber

Course Code Cate

go

ry

Course Title


Att

endance (

%)

Co

nti

nu

ou

s In

tern

al

assessm

en

t [T

heo

ry /

P

racti

cal]

(a)

(CIA

)


Grand Total

RG Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical tr

ain

ing

(CT

) P

ractical

(P)/

Researc

h

Pro

ject

Cre

dit

s ©

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory (b)


Theory

: a+

(b÷2)

= 1

00 P

ractical:

a +

c =

100

EST ESP

16 PBT15CT201 CT9 Immunotechnology 4 - - 4 60 - 60 80 50 100 - 100

17 PBT15CT203 CT10 Cancer Biology 3 - - 3 45 - 45 80 50 100 - 100

18 PBT15CT205 CT11 Environmental Biotechnology

2 - 1 3 30 30 60 80 50 100 - 100

19 PBT15CT207 CT12 Genetic Engineering 3 - - 3 45 - 45 80 50 100 - 100

20 PBT15DE209 DE2 Pharmaceutical Biotechnology [BDE 004]

3 - - 3 45 - 45 80 50 100 - 100

21 PBT15AE211 AE1 Scientific Writing and Research methodology

- 2 - 2 - 60 60 80 50 100 - 100

22 PBT15CL251 CL4 Genetic Engineering (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

23 PBT15CL253 CL5 Cancer Biology & Immunotechnology (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

Semester III Total 15 2 9 26 225 330 555 400 600 100 800



M Sc Biotechnology – Year 2; Semester-IV, 2016

Co

urs

e N

um

ber

Course Code C

ate

go

ry

Course Title

RG


Att

endance (

%)

Co

nti

nu

ou

s In

tern

al

assessm

en

t [T

heo

ry /

P

racti

cal]

(a)

(CIA

)


Grand Total

Lectu

re (

L)

Tuto

rial (T

)/ C

linic

al

train

ing (

CT

)

Pra

ctical

(P)/

Researc

h

Pro

ject

(RP

)

Cre

dit

s ©

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory (b)


Theory

: a+

(b÷2)

= 1

00 P

ractical:

a +

c =

100

EST ESP

24 PBT15DE202

DE3 (any one) - 3 - - 3 45 - 45 80 50 100 - 100

25 PBT15RP252

RP1 Dissertation & Viva

-

- 18 18 - 540 540 80 50 - 50# 100

Semester IV Total - 3 0 18 21 45 540 585 - 100 100 50 200

Grand total - 57 2 41 100 855 1290 2145 1250 1800 350 2500

a: CIA Theory & Practical (Eligibility to appear for university examinations is 40% & 80% attendance)

b: EST (Pass Min. CIA & ESE Aggregate: Grade B [50%])

c: ESP (Pass Min. CIA & ESE Aggregate: Grade B [50%])

# ESP- the total of 50 % is determined from the evaluation weighted at 25% for the dissertation and 25% for the viva voce (W: 25 % each)

Comparison of Credits for 2015 batch and 2016 batch of admissions

Year of admission

Sem1 Sem2 Sem3 Sem4 total

2015 29 24 26 21 100

2016 26 27 26 21 100



Electives / Ability Enhancement Courses (AEC) Offered – Faculty of BMST& R

LIST OF Discipline Specific Elective (DE) Courses Offered for M Sc Biotechnology Program

Dept. of Biotechnology

S. No.

Code Number

Course Title Credit Semester Level

Course Code Current Status

1. BDE001 Basic genetics 3 I PBT15DE107 Applicable for 2015 batch only

2 BDE002 Molecular Genetics 3 I PBT15DE107 Applicable for 2015 batch only

BDE002 Molecular Genetics 3 IV PBT15DE202 Applicable for 2016 batch onwards

3 BDE003 Advanced Microbiology

3 I PBT15DE107 Applicable for 2015 batch only

4 BDE004 Pharmaceutical Biotechnology

3 III PBT15DE209 Applicable for 2015 & 2016 batch onwards. No choice

5 BDE005 Biology of Viruses 3 IV PBT15DE202 Applicable for 2016 batch onwards

6 BDE006 Disorders of Metabolism

3 IV PBT15DE202 Applicable for 2016 batch onwards

7 BDE007 Molecular Diagnostics

3 IV PBT15DE202 Applicable for 2016 batch onwards

8 [BGE023] Bioinformatics

3 II PBT15DE110 Applicable for 2015 batch & 2016 batch onwards. No choice

LIST OF ABILITY ENHANCEMENT COURSES (AEC) Skill Enhancement Courses (SEC) - (SE)/ SL

9

Animal Cell Culture Technology

2 II PBT15SL154 Applicable for 2015 & 2016

batch onwards. No choice

10

Scientific Writing and Research methodology

2 III PBT15AE211 Applicable for 2015 & 2016

batch onwards. No choice

Generic Elective (GE) Courses Offered by Dept. of Biotechnology

S. No.

Code Number Course Name Credit Semester Level

Level UG/PG/I/D/Ph.D.

1 BGE027 Health care Biotechnology 3 2 UG

2 BGE029 Plant Tissue Culture Technology 3 2 UG

3 BGE030 Marine Biotechnology 3 2 UG

4 BGE036 Nanotechnology 3 3 UG

5 BGE032 Algal Biotechnology 3 3 UG

6 BGE031 Antimicrobial Agents 3 1 PG

7 BGE037 Alternative models for Experimental Toxicology

3 2 PG

8 BGE026 Nanodiagnostics 3 2 PG

M Sc. Biotechnology Syllabus:

First Year – Semester – I; Category: Core

Course Number

Course Code Course Title L T P C Total Hours

1 PBT15CT101 Biochemistry of Biomolecules 4 4 60 Objectives Learning outcomes

1. To impart knowledge on the chemical basis of life 2. Understand biochemical synthesis of biomolecules and energetics

On successful completion of the course, the student will be able to

comprehend the structures of the major classes of macromolecules



1. Biochemistry of Biomolecules

UNIT 1 Amino acids Classification, Structure & Chemical reactions of amino acids. Amino acid metabolism: biosynthesis & catabolism of amino acids -urea cycle, transamination, oxidative deamination, decarboxylation, ammonia transport.

UNIT 2 Carbohydrates Classification and reactions of Mono, Di- and Polysaccharides and biological functions of carbohydrates. Carbohydrate metabolism • Generation of energy and reducing power for biosynthesis: Glycolysis, TCA cycle, Glyoxylate cycle, HMP Shunt and Gluconeogenesis, Glycogenesis & Glycogenolysis. Cori‟s cycle

UNIT 3 Lipids

Classification, structure and functions of saturated and unsaturated fatty acids. Biological functions of Phospholipids, Glycolipids, Cholesterol, Steroid hormones and prostaglandins. Lipid Metabolism: Biosynthesis and oxidation of fatty acids - Oxidation of unsaturated and saturated fatty acids.

UNIT 4 Nucleic acids Classification, structure of purines and pyrimidines, nucleosides and nucleotides. Structure of DNA. RNA structure, types, Structure of t-RNA Biosynthesis of de novo purine and pyrimidine nucleotides. Regulation of nucleotide synthesis. Catabolism of nucleotides. Salvage pathway.

UNIT 5 Vitamins & Minerals Vitamins and Coenzymes – Classification, structure & properties, biological functions and their deficiency manifestations.

UNIT 6: Energy Flow: Generation of energy rich molecules and reducing power within a cell. Photosynthesis

UNIT 7 Respiration and Photorespiration Mitochondria and Electron transport chain, oxidative phosphorylation. Introduction to photorespiration; organelles involved; Reaction mechanism

Text Books 1. Fundamentals of Biochemistry: Life at the Molecular Level. 4th Edition, Donald Voet, Charlotte

W, Pratt, Judith G.Voet, 2011, John Wiley & Sons Inc. 2. Lehninger Principles of. Biochemistry. Fourth Edition. David L. Nelson and Michael M. Cox.

Fourth Edition.2005 3. Biochemistry. Sixth Edition, L.Stryer. W.H.2006,

Reference Books 1. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc. 2. Biochemistry. Fourth Ed. Zubay, 1998, Freeman and company 3. Biochemistry & Molecular Biology of plants, 2002, Buchanan B B, Gruissem W, Jones R L ASPP, USA.

Online Resources: http://nptel.ac.in/

First Year – Semester – I; Category: Core Theory

Course Number


2 PBT16CT103 Advanced Cell Biology 4 - - 4 60 Objectives Learning outcomes

To impart knowledge on the various aspects of cells like organelles, membrane, transport.

On successful completion of the course, the student will be able to - ● comprehend the cell organelle, cell membrane ● signal transduction and its implications ● cell cycle and its relevance

3. Advanced Cell Biology

UNIT 1 Study of cells: Cell theory; Diversity of cell size, shape, structure and organization in bacteria, yeast, plant and animal cells; Endosymbiotic theory. Methods for study of cells – cytological, Micrometry; cytochemical and biochemical methods – cell enumeration techniques – fluorescence activated cell sorter;



Microscopes: Stereo- and compound microscopes; Inverted microscopes; Microscopy: Phase –contrast; DIC; Darkfield; fluorescence; Confocal microscopy

UNIT 2 Structure, organisation and functions of cells: Cell organization - prokaryotic and Eukaryotic cells. Multicellular Organization; Cell adhesion; Cells- cell interactions; tissues; ECM in animal cells; Membrane junctions: tight junction, desmosomes, gap junctions; cell motility – cilia, flagella of eukaryotes and prokaryotes. Cell walls – structure of plant and bacterial cell walls

UNIT 3 Membrane structure and functions: Structure of Plasma membrane; fluid mosaic model; organelle membranes; membrane proteins Overview of membrane transport: Organization of transport Transport mechanism: Transport of nutrients, ions and macromolecules (active, passive, osmosis); transport across nucleus; facilitated transport, group translocation, Pumps • Carriers • General properties of ion channels • Ion channels in action • Water transport through aquaporins.

UNIT 4 Structure and function of cell organelles: Nucleus, Nucleolus, Endoplasmic reticulum, Golgi apparatus, Vacuoles, Lysosome, Ribosomes, Cytoskeletal systems; Cell adhesion; Cell motility- cilia, flagella

UNIT 5 Cellular energy transaction: Ultra – structure and role of mitochondria and chloroplasts

UNIT 6 Cell Cycle and Apoptosis: Cell cycle (Mitosis); Components of cell cycle – cyclins, CDKs; Cell cycle check points; Cell cycle regulation; Programmed cell death (PCD); types of PCD (Necrosis, Apoptosis, Autophagy, Anoikis)

UNIT 7 Cellular basis of differentiation and development – mitosis and Meiosis, gametogenesis and fertilization. Development of organisms: Molecular basis of pattern formation and developmental control in Acetabularia; multicellular organisms e.g., Drosophila or Arabidopsis.

Text Books: 1. Cell and Molecular biology. E.D.P. De Roberitis and E.M.F.DeRoberitis Jr.8

th Edition.

2. Molecular biology of the cell. B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts and J.D.Watson. Garland publishing, Inc., New York, 1994.

3. Cell and Molecular Biology. Philip Sheeler and Donald E. Bianchi – III Edition. Reference Books:

1. Molecular cell biology, J.Darnell, H.Lodish and D.Baltimore, Scientific American Books, Inc., USA, 1998.

2. Gene VII Benjamin Lewin, Oxford University Press, U.K., 2000. 3. Biochemistry & Molecular Biology. William H Elliott and D.C.Elliott, Oxford University Press,

2000. 4. Reproduction in Eukaryotic cells. DM Prescott, Academic Press. 5. Principles of Genetics by Gardner, Simmions&Snustad 1991.

Online resources:

1. NCBJ Book Store 2. http://nptel.ac.in/

First Year – Semester – I; Category Core

Course Number


3 PBT15CT105 Analytical Techniques 4 4 60 Objectives Learning outcomes

To understand basic concepts in various analytical techniques routinely used in laboratories.

On successful completion of the course, the student will be able to handle the equipments available - Identify the suitable and appropriate experiments for their research - gained sufficient knowledge about the assays and analyzing data

3. Analytical Techniques UNIT 1 Biological buffers and biochemical calculations: Buffers, pH, pKa, Acid, Base,

Henderson–Hasselbalch equation, Molarity, Molality, Normality, percentage purity. UNIT 2 Spectroscopic Techniques: Principle and application of UV-Vis, IR, Fluorescence

spectroscopy, Luminometry,



UNIT 3 Chromatography: Principles of Chromatography, Types of Chromatography- partition, adsorption, ionexchange, gel permeation and affinity chromatography, Applications of paper, thin layer - HPTLC, column chromatography - HPLC, GC, GC-MS, LC-MS-MS.

UNIT 4 Centrifugation: Principles of Sedimentation, Types of Centrifuges [differential, rate-zonal and density gradient centrifugation (CsCl, sucrose)]. Preparative and analytical centrifugation. Applications – Isolation of cells, sub-cellular organelles, viruses and macromolecules.

UNIT 5 Electrophoresis: Principle of electrophoresis, types of matrices, electrophoresis of proteins (SDS PAGE, Native PAGE), electrophoresis of nucleic acids (AGE, PAGE, PFGE).

UNIT 6 Radioisotopes: Nature and types of radioactivity, measurement of radioactivity, counters – GM, scintillation counters; autoradiography; biological applications, fluorography.

UNIT 7 Molecular Beacons and Quenchers [Principles and applications]: BRET; FRET; Fluorophores; chemiluminescence, phosphorescence, bioluminescence; Plasmon resonance

Text Books 1. Principles and Techniques of Biochemistry and Molecular Biology – Keith Wilson and John

Walker. 7th Edition, Cambridge University Press, 2010.

2. Medical Biomethods handbook by John M.Walker & Ralph Rapley, Humana Press. 3. Tietz Textbook of Clinical Chemistry and Molecular Diagnosis by Carl A. Burtis, Edward R.

Ashwood, David E.Bruns – Elsevier 4. Principles of Bioche mistry. A.Lehninger et al,6

th Edition, UBS publishers.

5. Biochemistry. L.Stryer. W.H.1999, Freeman and company. Reference Books

1. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc. 2. General Chemistry, Linus Pauling, W.H. Freeman and company.

Online Resources: 1. mcdb-webarchive.mcdb.ucsb.edu/.../biochemistry/.../website-tourf.htm 2. www.biochemweb.org/ 3. http://golgi.harvard.edu/biopages.html 4. http://nptel.ac.in/

First Year – Semester - I Category: Core Theory

Course Number


4 PBT16CT107 Advanced Microbiology

3 - 3 45

Objectives Learning outcomes

To understand the fundamental principles of microbiology and their identification

On successful completion of the course, the student will be able to understand

- microbial diversity; physiology and nutrition; - Identification of microbes using modern techniques

Course Number Course code Category Course title

L T P C L/T/P Hours

Total Hours

5 PBT15GE109 Generic Electives

(any one)

3

3 45 45



4. Advanced Microbiology UNIT 1 Introduction to Microbiology • Microbial diversity, General and distinctive characters of

groups of fungi, algae and protozoa Principles of bacterial taxonomy, classification of bacteria; General characteristics of the groups - Microscopy, Growth & Biochemical characteristics;

UNIT 2 Introduction to Fungi: distribution, importance, structure, nutrition. Reproduction – asexual and sexual; Lifecycle of Yeast; Neurospora and Aspergillus; division Myxomycota- life cycle of Dictyostelium discoideum

UNIT 3 Microbiological techniques & Nutrition Cultivation of aerobic and anaerobic organisms, Preservation and maintenance of cultures. Culture Media: Synthetic media, Complex media, Selective media & differential media. Microbial Nutrition; Fungal cultivation methods

UNIT 4 Growth and Multiplication of Bacteria Microbial growth – definition, Techniques of measurement of growth and enumeration. Growth curve. Batch culture, continuous culture (Turbidostat and chemostat) and synchronous growth cultures; Environmental factors affecting growth.

UNIT 5 Microbial metabolism Bacterial Chemolithotrophy: ammonia oxidation by members of Genus Nitroso group, nitrite oxidation by Nitro group of genera • Diazotrophs; Oxidation of molecular hydrogen by Hydrogenomonas species. Ferrous and sulfur/sulfide oxidation by Thiobacillus species. Photosynthesis in microorganisms.

UNIT 6 Bioinformatics in microbiology: Using Bioinformatics Tools (ResFinder, SRST, PHAST, PATRIC Blast Search, blastn, PGAT - Prokaryotic Genome Analysis Tool etc.); New approaches to bacterial taxonomy including numerical and molecular taxonomy; Identification using conserved Sequences; Multi-locus sequence typing, Identifying Resistance Genes; Antibiotic susceptibility Testing; Molecular methods used for microbial diagnostics.

UNIT 7 Economic Importance: Overview of the applications of microbes – food and Industrial Microbiology Some examples of microbial uses: edible mushrooms; Biofertilizers (Cyanobacteria, Phosphobacteria; Azotobacter, Azospirullum), Cultivation of Biopesticides in integrated agriculture management: biopesticides - Trichoderma; Bt based biopesticide.

Text Books: 1. Text Book of Microbiology by R. Ananthanarayan and C.K. Jayarama Panicker (Orient Longman

Ltd.) 2. Microbiology by Pelczar Jr. M. J. Chan. E. C. S and Krieg N. R (Mc Graw Hill Inc. Newyork) 3. Mackie and Mc Cartney‟s Text Book of Microbiology – Robert Cruikshank.

References: 1. Microbiology,5th edition by Prescott L. M, Harley J. P and Klein D. A., (Wm. C. Brown publ.). 2. General Microbiology, 5th edition by Stanier R. J. Ingraham J. L. Wheelis M. L and Painter P. R.

(Macmillan Education ltd.)

First Year – Semester - I category- Core Lab

Course Number


6 PBT15CL151 Biomolecules & Analytical Techniques(Practical)

8 4 120


To teach the fundamentals and nature of biomolecules including their analysis using relevant techniques


- the properties of biomolecules that are used for their analysis

- the principle concepts in using analytical and preparatory techniques

- how to quantify and assay for a biomolecule



3. Brock‟s Biology of Microorganisms, 9th edition by Madigan Michel (2000), (Prentice Hall Publications)

4. Textbook of Microbiology. Freeman B.A.Burrows Saunders HB company, 1985. Online Resources: 1. ResFinder 1.3 (Acquired antimicrobial resistance gene finder) 2. [http://cge.cbs.dtu.dk/services/ResFinder/] 3. http://pubmlst.org/plasmid/Plasmid MLST Databases; (MultiLocus Sequence Typing) 4. http://cge.cbs.dtu.dk/services/MLST/ 5. SRST on SourceForge: http://srst.sourceforge.net

6. Biomolecules and Analytical Techniques (Lab)

No. Lab title

1. Buffers and buffering capacity – pKa of conjugate acids and bases 2. Visible Absorption Spectroscopy – Instrumentation and Basic concepts of Beers Law using

colored compounds - potassium permanganate; potassium dichromate; red dye 3. Using colorimetry for Clinical Biochemistry: Estimation of albumin by BCG method 4. Visible Spectroscopy and its utility: Estimation of Cholesterol in unknown samples 5. Visible Spectroscopy and its utility: Estimation of Glucose in standards and unknown

samples using „Somogyi-Nelson method‟ 6. Uv Absorption Spectroscopy – Sodium Salicylate or aspirin; chlorophyll; BSA; p-

hydroxybenzaldehyde; vanillin; Proteins 7. Chromatography: TLC of lipids/ curcumin/ pepper/ pigments 8. Paper chromatography of leaf pigments 9. Chromatography: Column Separation of leaf pigments using silica gel 10. Principle of Separation of nucleic acids using agarose gel electrophoresis

Text Books:

1. Principles and Techniques of Biochemistry and Molecular Biology – Keith Wilson and John Walker. 7th

Edition, Cambridge University Press, 2010.

2. Biochemistry. Zubay, 1998, Freeman and company.

Reference Books

1. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc.

2. A Biologist‟s Guide to Principles and Techniques of Practical Biochemistry, K. Wilson & KH Goulding,

ELBS Edition, 1986.

3. Tools of Biochemistry by T.G. Cooper.

Online Resources:

1. mcdb-webarchive.mcdb.ucsb.edu/.../biochemistry/.../website-tourf.htm 2. www.biochemweb.org/ 3. http://golgi.harvard.edu/biopages.html 4. webarchive.mcdb.ucsb.edu/sears/biochemistry/info/website- 5. mscbiotech.net

First Year – Semester - I category: Core Lab

Course Number


7 PBT16CL153 Biology of cells and microbes (Practical)

8 4 120


To impart knowledge on techniques used to study cells and microbes

On successful completion of the course, the student will be able to - Understand the basics of techniques to study cells Basics of microscopy Aseptic techniques and microbial culture methods

http://srst.sourceforge.net/



Practical Manuals:

1. Laboratory Manual in Microbiology, Gunasekaran.P (2009), New Age international Pvt Ltd

Publishers, ISBN-10: 8122407838.

2. Cell Biology Protocols, J. Robin Harris, John M. Graham, David Rickwood,(2006), Wiley, ISBN-

9780470847589

3 Microbiology: A Laboratory Manual, James G. Cappuccino, Natalie Sherman,(2013), Pearson

Education, Limited, ISBN-9780321864864.

Online resources:

1. http://web.calstatela.edu/faculty/rnissen/biol380/BIOL380LabManualSP10.pdf

2. http://homepages.uconn.edu/~mb2225vc/MCB_2225/Course_overview__h1_MCB2225

3. http://study.com/academy/topic/microbiology-laboratory-techniques.html

First Year – Semester – II; Category: Core theory

Course Number

Course Code Course Title L T P

C

Duration (hrs )

8 PBT15CT102 Molecular Biology 4 4 60 Objectives Learning outcomes

To impart knowledge on the concept of gene and its biological activities in cells

On successful completion of the course, the student will be able to understand what genes are; how they are inherited, how they control cellular activity and they respond to environment

8. Molecular Biology

UNIT-1 Gene concept: Nature of genetic material- Evidence to prove DNA and RNA as genetic material. Gene as unit of expression: Modern concept of gene, types of genes (constitutive, structural, regulatory, luxury, overlapping and others). Biological features of DNA; Properties and features of Plasmids; DNA isolation and purification; DNA Analysis- quantification and agarose gel electrophoresis

UNIT-2 Replication and its regulation: Replication of DNA - Enzymology of replication in eucaryotes and procaryotes; fidelity of DNA replication. Models of replication: Col E1 plasmid, Ǿ X174, Lambda phage; D-loop; inhibitors of DNA replication.

No. 7. Biology of cells and microbes 1. Microscopy: Bright field and phase contrast with representative cell types- bacteria, fungal,

algal, plant and animal cells 2. Micrometry for cell enumeration

3. Micrometry for cell size determination

4. Mitosis stages in Onion Root Tip - Histochemical techniques

5. Histochemical analysis of Meiosis – Meiosis slides 6. Osmotic analysis or Plasmolysis – potato or onion cells 7. Bacterial Cell motility- hanging drop method 8. Isolation of cell types using gradient centrifugation and their staining 9. Polytene chromosomes of Salivary glands - Chironomus or Drosophila

10. FACS – demonstration Microbiology: 11. Sterilization techniques – Principles and sterility testing using bacteria - autoclaving, heat

sterilization, filtration, UV irradiation and Chemicals 12. Media and Slant preparation: Liquid media, Solid media 13. Isolation and cultivation of pure cultures of bacteria and fungi 14. Microscopy and staining of bacteria: Simple staining, differential staining, Capsule staining,

Acid –fast staining and Spore staining 15. Kirby-Bauer method of antibiotic susceptibility test using bacteria and fungi 16. Biochemical test for identification of bacteria: Catalase test, Oxidase test, IMViC test, Starch

degradation test, Carboydrate fermentation

17. Growth curve determination of bacterial cultures 18. Estimation of Colony forming units of bacteria

https://en.wikipedia.org/wiki/Chironomidae



UNIT-3 Transcription: Structure of prokaryote and eukaryote promoters, RNA polymerases, General and specific transcription factors; process of transcription; Processing of RNAs- mRNA, rRNA, t-RNA – Mechanism, splicing, catalytic RNA, editing, nuclear export of RNA, Inhibitors of transcription

UNIT-4 Protein Synthesis, Assembly and Degradation: Prokaryotic and Eukaryotic translation •

Ribosomes; deciphering genetic code, types of RNA and their role in protein synthesis,

mechanism of initiation, elongation and termination. Post-translational protein modification

e.g. glycosylation; protein degradation; Inhibitors of translation.

UNIT-5 Gene regulation: operon and regulon concept, gene expression of lac, Catabolite Activator protein; galactose, tryptophan operons and mal regulon; DNA binding proteins; Phase variation in Salmonella; bacterial sporulation; regulation of Lambda lysogeny; riboswitches; epigenetics- acetylation of histones

UNIT-6 Gene Regulation by Signal Perception and Transduction: Overview of signal transduction • Receptors • G-proteins and phospholipid signaling • Cyclic nucleotides • Calcium • Protein kinases: primary elements in signal transduction - Tyrosine Kinase receptor, Jak/STAT; Non-Kinase (wnt, hedgehog); Hormones (lipophilic, peptide, growth factors).

UNIT 7 Gene regulation: nuclear transport regulation in HIV; genome imprinting; DNA methylation; antisense RNAs – (Excludons); RNAi; CRISPR.


th Edition.




2. Gene VII Benjamin Lewin, Oxford University Press, U.K., 2000. 3. Principles of Genetics by Gardner, Simmions & Snustad 1991.

Online Resources: 1. NCBI Book Store 2. http://nptel.ac.in/

First Year: Semester II; Category: Core Theory [Lab cum theory]

Course Number

Course Code Title L T P C Total Hours

9 PBT15CT104 Bioprocess Technology 4 4 60

Objectives Learning Outcomes

To understand the up and downstream processing

in the chemical, pharmaceutical, and food industries

Students will be aware of various methodologies for biomass production and product isolation using various analytical methods

9. Bioprocess Technology Unit 1 Microbial Technology: Food and Industrial Microbiology - An Introduction; Potential

Sources of Microbial Enzymes • Screening for industrially important and novel Enzymes. Introduction to Bioprocess Technology: Outline of an integrated bioprocess and various unit operations. Media for Industrial fermentation, Filter sterilization of Air; Batch and continuous sterilization of media

Unit 2 Fermentation & Bioreactor design: Types of fermentations- Aerobic and anaerobic fermentation, Submerged and solid state fermentation; Factors affecting submerged and solid state fermentation; Substrates used in SSF and its advantages Bioreactor design: Design and components of a bioreactor; Tank & Airlift bioreactors - design, stability and functions of their components; Online Measurement and control of bioprocess parameters. Variations in bioreactor design, Immobilized bioreactors; Photobioreactor



Unit 3 Scale up & Industrial scale production of bio-chemicals

Principles of scale up; scale up and scale down methods;

Unit 4 Downstream processing: Introduction, removal of microbial cells and solid matter, foam

separation, precipitation, filtration, centrifugation, cell disruptions, liquid-liquid extraction,

chromatography, drying and crystallization

Unit 5 Extraction and purification of microbial enzymes Importance of enzyme purification, Extracellular and intracellular enzymes. Enzyme fractionation by precipitation (using Temperature, salt, solvent, pH, etc.), affinity chromatography and other special purification methods. Enzyme crystallization techniques. Criteria of purity of enzymes • Immobilization of microbial enzymes – principles and applications

Unit 6 Industrial scale production of Ethanol, Citric acid, Glycerol, Amino acid-lysine, Penicillin antibiotic, Vitamins B12.

Unit 7 Applications of microbial enzymes: Microbial enzymes in textile, leather, wood industries and detergents. Enzymes in clinical diagnostics • Enzyme sensors for clinical processes and environmental analysis • Enzymes as therapeutic agents

Text Books 1. Principles of fermentation Technology, second edition, Stanbury, P.F, Whitaker, A and Hall S.J.(1999) - by Butterworth-Heinemann,ISBN-13: 978-0-7506-4501-0, ISBN: 0-7506-4501-6 2. Biotechnology: A Textbook of Industrial Microbiology, Crueger W. and Crueger A. (2000), 2nd Edition, Panima Publishing Corporation, New Delhi. 3. Industrial Microbiology: Fundamentals and Applications, Agrawal A.K., Parihar, P (2008), Publisher: Agrobios (India), ISBN 13: 9788177542332 Reference Books 1. Biochemical Reactors by Atkinson, B,(1974), Piod Ltd. London, ISBN-13: 978-0850860429. 2. Biochemical Engineering Fundamentals, James Edward Bailey, David F. Ollis, 1986, ISBN- 13:9780070032125 Online Resources:

1. http://www.eng.umd.edu/~nsw/ench485/ench485.htm 2. http://www.engineersirelandcork.ie/downloads/Biopharmaceuticals

10. Biological Calculations & Biostatistics

UNIT 1

Basic mathematics in Biology – concentration of solutes in solution - Molarity and normality; Percent weight, Dilutions and proportions. Measurements and Units: conversion of units and concentrations Fractionals, decimals and percentages.

UNIT 2

Exponents - Logarithms and Exponents: e.g., Growth Curves; concentration of DNA Ratio and proportions- amounts, volumes and concentrations e.g CFU/ mL; PFU/ mL; HA units; Cell counts; Reaction mixes and others

UNIT 3 Basics of Biostatistics: Frequency distributions, measures of central tendency: mean, mode and median. Measures of dispersion - Standard deviation, Quartile deviation. Coefficient of dispersion, coefficient of variation

Unit 4 Sampling methods: Probability, Non-probability based method Hypothesis Testing: Introduction - Hypothesis testing

First Year – Semester – II; Category: Core

Course Number


10 PBT15CT106 Biological Calculations & Biostatistics

4 4 60


To provide the basics of experimental methods, design, planning, carrying out scientifically research.

On successful completion of the course, the student will be able to -

Understand simple calculations

How to plan and execute research designs

Analyse data, interpret, and present information

Publishing research data

Calculate; analyse and compare observed data; perform simple sums in proportions and algebraic functions

http://biblio.co.uk/isbn/9788177542332.html

http://www.barnesandnoble.com/c/james-edward-bailey

http://www.barnesandnoble.com/c/david-f.-ollis



UNIT 5 Tests of significance: Students‟ t- test; X2 ; ANOVA

UNIT 6 Correlation & Regression Analysis Correlation Analysis - Types of correlation - Rank Correlation Coefficient - Regression analysis - Types of Regression -Assumptions - Comparison to Correlation

UNIT 7 Quantitative Data presentation: Generating different graphs and charts - Straight –Line Graphs- Calibration Curves and Linear rates e.g. Km and V max; Nonlinear rates of change: Graphs, Transformations and Rates; Relative Units

TEXT BOOKS:

1. Research Methodology: Methods and Techniques. 2nd

Ed. 1990, C R Kothari, Wiley Eastern Ltd.,

New Delhi, India

2. Basic Calculations for Chemical and Biological Analysis, 2nd Edition

Copyright © 2000 AOAC INTERNATIONAL. 238 Pages. Softcover. ISBN 0-935584-69-2

REFERENCE BOOKS:

1. Elementary statistical methods. Reprinted 2002. Gupta SP Nutan Chand and Sons, New Delhi, India.

2. Basic calculations for Chemical and Biological analysis, 2nd

Ed. 2000, AOAC International, 238p;

ISBN: 0-935584-69-2

3. Statistics: Theory and Practice. 1989. Shukla N C and SS Gulshan. S Chand & Co. Ltd., New Delhi,

India.

ONLINE RESOURCES:

Math.com - World of Math Online: www.math.com

http://www.basic-mathematics.com/math-websites.html

First Year – Semester –II; Category: Core

Course Number


11 PBT16CT108 Protein Biology 3 3 45


To impart knowledge on Structure; functions and analysis of proteins in cells; enzymes and its analysis

On successful completion of the course, the student will be able to ● comprehend the structure of the proteins ● functions of proteins and enzymes ● analysis of proteins by various methods ● bioinformatics tools for protein analysis

11. Protein Biology

UNIT 1 Proteins and peptides: Peptides are Polyelectrolytes;

Diversity in function - Structural proteins; soluble proteins; regulatory proteins Structural proteins: cytoskeleton- Protein Interactions Modulated by Chemical Energy: Actin, Myosin, and Molecular Motors; Membrane proteins and their functions; Soluble proteins: catalyze metabolic reactions; Biosignaling; Reversible Binding of a Protein to a Ligand - transport of oxygen; Complementary Interactions between Proteins and Ligands: Immune functions Regulatory proteins: Repressors; activators; enhancers Biologically active peptides

UNIT 2 The Four Orders of Protein Structure - Primary; secondary; tertiary and Quarternary; Ramachandran plot; protein structure and prediction (swissprot); Protein Denaturation and Folding; disorders of protein misfolding- prions; Alzheimer‟s; maturation and cleavage of proteins e.g., collagen

UNIT 3 Introduction to Enzymes General properties of enzymes, functional organization of enzyme- domains, (multi enzyme complex); active site of enzyme; Mechanism of enzymes catalysis Nomenclature and classification; Coenzymes, Metal ions in enzyme catalysis and Isoenzymes

UNIT 4 Basic Concepts in Enzyme Kinetics Free energy, activation energy, enzyme substrate complex, Kinetics- . Michaelis – Menten kinetics; kinetic parameters- Km, Vmax, Kcat; Lineweaver Burke plot, Enzyme assay- measurement of enzyme activity- specific activity, enzyme units- IU, katal Factors affecting enzyme activity; Enzyme inhibition – types of inhibition.



UNIT 5 Purification of enzymes: Enzyme sources- plant, animal and microbial. Extraction of Membrane bound proteins, intracellular and extracellular enzymes, precipitation; Purification table. Separation and analysis of proteins: Isoelectric Focusing (IEF); Protein sequencing by Edman degradation – outline

UNIT 6 Functional roles of peptides: function of signal peptides – signal hypothesis – protein targeting - Protein localization and translocation, mechanism of translocation Protein modifications (e.g., phosphorylation, acetylation) and their functions; Protein – Protein interactions;

UNIT 7 Proteomics: Fundamentals; Techniques; Analysis and applications - Separation by 2D electrophoresis; MALDI-TOF, LC-MSMS, Protein in-silico tools - retrieval of sequences (FASTA), homology search, clustalW, phylogenetic analysis for protein (Mega6).

Text Books 1. Biochemistry by Harper, 2. Biochemistry - Donald Voet and Judith G.Voet, 4

th Edition, 2011, John Wiley & Sons Inc.

3. Principles and Techniques of Biochemistry and Molecular Biology – Keith Wilson and John Walker. 7

th Edition, Cambridge University Press, 2010.

4. Biochemistry. L.Stryer. W.H.1999, Freeman and company. 5. Biochemistry. Zubay, 1998, Freeman and company.

Reference Books 1. Principles of Biochemistry. A.Lehninger et al,6

th Edition, UBS publishers

2. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc. 3. General Chemistry, Linus Pauling, W.H. Freeman and company. 4. Organic Chemistry, DJ Cram and GS Hammond, McGraw Hill.

Online Resources http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html https://www.chem.purdue.edu/courses/chm333/web%20Site%202.htm http://www.wiley.com/legacy/college/boyer/0470003790/links/links_list.htm#visual http://nptel.ac.in/

12. Bioinformatics [Lab cum Practicals] #

UNIT 1 Computer Fundamentals

Characteristics of computer, history, generations, types, classification – Hardware,

Software; Operating System - Linux, Windows. Internet and search engines, Office

Packages - MS Word, MS Excel, MS PowerPoint, internet.

UNIT 2 Biological Databases

Bioinformatics and its relation with molecular biology, Molecular Resources, Primary &

Secondary databases, Public databases - NCBI, EBI, DDBJ, Database File formats,

Submission & retrieval tools

Sequence Alignment

Introduction, Sequence similarity, identity and homology, Dot matrix analysis, Local and

global alignments, Sequence based searches; BLAST– Introduction, Definition, Types,

Scoring matrices

First Year – Semester – II; Category: Discipline Specific Elective; Department of Bioinformatics

Course Number


12 PBT15DE110 [BGE023]

Bioinformatics 2 2 3 60


To know the importance of computers in biology

To understand software tools for biological sequence analysis

To learn the concepts associated to Genomics and apply the same invarious fields


Get to know effective use of Office package

Understand the biological sequence analysis

The student will be able to understand the

concepts associated to Genomics and apply

the same in various fields

http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html

https://www.chem.purdue.edu/courses/chm333/web%20Site%202.htm

http://www.wiley.com/legacy/college/boyer/0470003790/links/links_list.htm#visual



Multiple Sequence Alignment & Phylogeny

Introduction, Progressive alignment method - ClustalW, Phylogenetic trees - types &

topology, Methods - Maximum Parsimony, Distance methods, Maximum Likelihood

approach.

UNIT 3 Genomics

Introduction – Evolution – Genome Organisation of Prokaryotes, Eukaryotes &

Organelles – Human Genome Project − Genome Annotation – SNPs & Mutation –

Gene & Genome Duplication – Gene Loss

UNIT 4 Proteomics

Components – Protein Str. Prediction – Mass Spec - Analysis in Proteomics – Disease

link

UNIT 5 Computer Aided Drug Design

Principles - Molecular Modelling – docking – QSAR - Applications

UNIT 6 Practical: 30 Hours

1. MS Office Packages

2. Submission & Retrieval tools

3. Sequence Editing & Alignment

4. BLAST

5. Phylogenetic analysis

6. Genome Browsers

7. Model Organism Databases

8. Mutation Databases

9. Proteomics & Structural Bioinformatics ( Demo only )

Reference Books:

1. Introduction to computers & Data processing – Shelly, Gray. B2. Mastering Microsoft office 2007 – Alison Balter‟s

2. Bioinformatics sequence and Genome analysis – David W. Mount, 2004, 2ed

3. BLAST. The Definitive Guide. Basic Local Alignment Search Tool – Korf, Yandell, Bedell

4. Introduction to Bioinformatics - Attwood, Smith, Parry-Smith

5. Introduction to Genomics, Arthur M. Lesk, 2007, Oxford UniversityPress.

6. Handbook of Comparative Genomics – Principles & Methodology 2003 Saccone & G.Pesole (Publication) Wiley-Liss

7. Microbial Functional Genomics – 2004 Jizhong Zhou, Dorothea K.Thompson, Ying Xu & James.M.Tiedje (Publication) Wiley-Liss

Online Resources: www.ncbi.nlm.nih.gov www.embl.org # - The Internal Assessment in the form of a practical exam maybe included as no separate ES exams is scheduled for the practicals.

Department of Biotechnology – Year 1 – Semester-II

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13 PBT15GE112

Generic Elective

Choice - (any One) 3 3 45 45



No. 15. Protein Methods (Lab)

1 Cell disruption techniques – mechanical and enzymatic - estimation of total proteins -Biuret or BCG method

2 Purification of proteins and determining the fold purification: Enzyme isolation from bacteria or fungi or plants e.g. amylase; protease; lipase (any one) Large scale Extraction of crude enzyme

3 Precipitation of proteins (Salt or TCA or Acetone) (Ammonium sulphate) precipitation of proteins and Dialysis

4 DEAE Column purification of proteins

5

Enzyme Activity assays: Estimation of Sugar release by „Somogyi-Nelson method‟ using crude Amylase enzyme Fold purification of enzyme activity or Estimation of proteases by Kunitz method or Estimation of lipase using tributyrin.

6 Method of immobilization of enzymes – algination and its acitivty 7 Enzyme Kinetics: To determine the activity of amylase/ lipase/ protease – Vmax, Km 8 To determine inhibition of enzyme using specific inhibitors 9 Characterization of enzymes - pH optima 10 Characterization of enzymes- temperature optima

11 Separation of GST protein by affinity purification (glutathione S sepharose column) and analysis using SDS PAGE electrophoresis

12 Native gel Electrophoresis of protein / enzyme [zymogram] e.g., amylase or Peroxidase and activity staining

Text Books:

1. Principles and Techniques of Biochemistry and Molecular Biology – Keith Wilson and John Walker. 7th

Edition, Cambridge University Press, 2010.

2. Biochemistry. Zubay, 1998, Freeman and company.

Reference Books

1. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc.

2. A Biologist‟s Guide to Principles and Techniques of Practical Biochemistry, K. Wilson & KH Goulding,

ELBS Edition, 1986.

3. Tools of Biochemistry by T.G. Cooper.

Online Resources: 1 mcdb-webarchive.mcdb.ucsb.edu/.../biochemistry/.../website-tourf.htm 2 www.biochemweb.org/ 3 http://golgi.harvard.edu/biopages.html 4 webarchive.mcdb.ucsb.edu/sears/biochemistry/info/website- 5 mscbiotech.net

First Year – Semester – II; Category: Core Lab

Course Number


14 PBT15CL152 Protein Methods (Practical)

8 4 120


To provide basic understanding of protocols and applications of proteins


comprehend basic concepts of isolating, purifying and characterizing proteins

understand the principles and applications of these technologies



13. Animal Cell Culture Technology

UNIT 1 History & Basics

Importance and history of cell culture development. Structure and Organization of animal cells; tissues and Organs

Methods and Techniques

Requirements for animal cell culture technology; Primary and established cell line cultures; Growth medium, balanced salt solutions and their properties. Chemical, physical and metabolic functions of different constituents of culture medium. Role of Carbon dioxide, serum supplements and defined media and their application.

UNIT 2 Biology and characterization

Characterization of the cultured cells, Morphology, measuring parameters of growth; Basic techniques of mammalian cell culture in vitro; differentiation, maintenance of cell culture. Measurement of viability and cytotoxicity and primary culture. Three dimensional culture and tissue engineering

Stem Cells: Types; Isolation and characterization

UNIT 3 Applications

Cell Culture Technology, Cell culture based vaccines, expression of recombinant proteins in mammalian and avian cell lines and hybridoma technology. Assisted reproductive technology, embryo biotechnology, endocrine therapeutics, gene knock outs and Cloning of domestic animals. Conservation of endangered species.

UNIT 4 Lab Course in Animal Cell Culture techniques: Packing and sterilization of glass and plastic wares for cell culture; Preparation of reagents and media for cell culture. Initiation of Primary cell cultures from chick embryo/ zebra fish / Drosophila eggs/ Isolation and cultivation of lymphocytes.

UNIT 5 Secondary culture and passaging of cell lines; Cultivation of continuous cell lines. Quantification of cells by trypan blue exclusion dye. Isolation of DNA from cells and analysis (Quantification; agarose gel electrophoresis).

Text Books: 1. Culture of Animal Cells, (3rd Edition), F1. Ian Freshney. Wiley-Liss. 2. Animal Cell Culture – Practical Approach, Ed. John R.W. Masters, OXFORD. References 1. Cell Culture Lab Fax. Eds. M. Butler & M. Dawson, Bios Scientific Publications Ltd., Oxford. 2. Methods in Cell Biology, Vol. 57, Animal Cell Culture Methods. Ed. Jenni P. Mather and David Barnes. Academic Press. Online Resources:

2. http://userpages.umbc.edu/~jwolf//method5.htm 3. http://nptel.ac.in/

First Year – Semester – II; Category: Skills enhancement Lab

Course Number


15 PBT15SL154 Animal Cell Culture Technology (Practical)

1 2 2 45


To provide basic understanding of protocols and applications in animal cell culture


comprehend basic concepts of establishing animal cell cultures

understand the principles and applications of these technologies



Second Year: Semester III- Core Theory

Course Number


16 PBT15CT201 Immunotechnology 4 4 60


To enable students to acquire the requisite highly specialized scientific knowledge, clinical and laboratory skills in immunology.

The student must have - Understood the principles of immunology Understood methods of studying immune reactions Applications of this core science

16. Immunotechnology UNIT 1 Introduction, Cells of the Immune system: B-Lymphocytes, T-Lymphocytes,

Marcophages, Dendritic cells, Natural killer and Lymphokine activated killer cells. Eosinophils, Neutrophils and Mast cells. Innate and acquired immunity; organization and structure of primary and secondary lymphoid organs. Structure, classification and biology of antigens

UNIT 2 Antigen processing and presentation, generation of humoral and cell mediated immune responses, Activation of B-and T- lymphocytes, isolation and separation of lymphocytes, regulation of immune response.

UNIT 3 Structure, classification and functions of Immunoglobulins. Extraction and purification of immunoglobulins; cytokines and their role in immune regulation

UNIT 4 Cell mediated cytotoxicity: Mechanism of T cell and NK cell mediated lysis, antibody dependent cell mediated cytotoxicity, macrophage mediated cytotoxicity. Major histocompatibility complex, BCR and TCR, generation of diversity, complement system.

Unit 5

Hypersensitivity reactions. Antibody mediated Type I anaphylactic reactions, Antibody

mediated Type II cytotoxic reactions, Type III – Immune complex reactions, Type IV T

cell mediated delayed type hypersensitivity.

Transplantation, graft Vs Host reaction, Tumor immunology, host tumor relationship,

immunodiagnosis and immunotherapy

Autoimmunity, AIDS and other primary and secondary Immunodeficiencies.

Unit 6

Antigen and antibody interactions – agglutination, precipitation, complement fixation, neutralization, Immunoelectrophoresis, immunofluorescence, ELISA, RIA, Immunohistochemistry.

Unit 7 Vaccines and Immunoprophylaxis – objectives of immunization, types of vaccines. Types of antisera immunization schedule, Hybridoma technology and monoclonal antibodies and their uses.

Text Books: 1. Kuby immunology. R.A. Goldsby. Thomas J. Kindt, Barbara, A. Osborn. 2000 2. Fundamentals of Immunology, William Paul 1996 Raven press. 3. Ivan M Roitt. 2002. Essentials in Immunology 9th edition. Blackwell Scientific publishers Reference Books: 1. Cooper E. L. (1982) General Immunology, Pergamon press. N.Y. 2. Geoffry sunshine, Sidney leskowitz (1996) Immunology a short course, third edition, A John Wiley &

sons inc. publication. 3. Nandini Shetty (1999) Immunology introductory text book. New age international (p) Ltd publishers Online Resources: 1. http://www.immunologylink.com 2. http://www.roitt.com

Second Year: Semester III- Core Theory

Course Number


17 PBT15CT203 Cancer Biology 3 3 .45


To provide new knowledge concerning cancer development and progression Understand mechanistic processes that drive tumor initiation, progression and invasion

On successful completion of the course, the student will be able to understand terms in cancer biology, causes of cancer, the technologies used in cancer research and diagnosis



17. Cancer Biology

UNIT 1 Introduction and Basics Definitions- Cancer, tumour, neoplasia, benign and malignant, metastatic tumour, carcinogen, oncogene, tumour suppressor gene. Basic blood cell morphology and classification of leukemia. Types of growth disorder, dysplasia and carcinoma in situ, control of normal cell growth and behavior. Tumor metastasis, controlling metastasis, tumor vasculature and angiogenesis. Estrogen receptors (ER/PR) related to aging and cancer.

UNIT 2 Genetic pathways & Cell cycle Phenotype of cancer cell lines, their growth and maintenance. Genetic pathways that protect cells from uncontrolled growth and types of genes- Cell cycle control genes. Pathways that regulate tumor initiation and/or its progression; the role of tumor suppressors in the cell cycle, Contact inhibition of cell division and movement. Growth factors in the biology of cancer.

UNIT 3 Oncogenes and Apoptosis Oncogenes, Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins, checkpoint pathways induced in response to DNA damage; apoptosis; defective apoptotic/cell proliferation pathways leading to cancer.

UNIT 4 Carcinogenesis

Carcinogens- types and identification. Initiation, promotion and progression; Environmental factors and influences: Radiation carcinogenesis: Ionizing and non- ionizing radiation associated with carcinogenesis. Chemical carcinogenesis: tumor promoters and carcinogens. Biological-Viral RNA & DNA oncogenic virus. Free radical induced damages and antioxidants.

UNIT 5 Genomic instability & tissue specificity Genetic predisposition to cancer, Types of genomic instability: instability of short, repetitive sequences due to loss of DNA mismatches repair and dysfunction of telomeres; Tissue Specificity and Cells of origin- mouse models for cancer. Cancer Genome Project – an insight into genomic instability and sequencing

UNIT 6: Tumor Biomarkers Principles of tumor markers and circulatory tumor markers, Cytogenetic markers, Tumor cell associated glycoconjugates; epigenetic markers; mitochondrial markers, metabolic markers and therapeutic markers.

UNIT 7 Cancer Diagnosis & Therapeutics Histopathological diagnosis of cancer and staging of the disease. Different types of cancer and management. Immunohistochemistry -visualization and differentiation of cancer cells; Cytological detection-FNAC and guided biopsies. Chemotherapy and radiation therapy: Anticancer drugs and their mechanism of action, and FDA approval procedures; Radiotherapy, Photodynamic therapy Antiangiogenesis therapy; MoAb, Cancer vaccines by passive immunotherapy.

Text Books 1. Weinberg RA (2006), Cancer Biology. Publisher- Garland Science. 2. Mc kinnell RG, Parchment RE, Perantoni AO and Pierce B (1998), The Biological Basis of Cancer. Cambridge University Press. Reference Books: 1. Kleinsmith JL (2005), Principles of Cancer Biology, Benjamin Cummings Publication. 2. Franks LM and Teich NM (1997), Introduction to the Cellular and Molecular Biology of Cancer (3

rd

edition), Oxford University Press 3. Tumor Markers: Physiology, Pathobiology, Technology, and Clinical Applications. Eleftherios P. Diamandis. American Association for Clinical Chemistry Press, 2009- Medical - 541 pages

Online Resources 1. http://www.insidecancer.org/ 2. http://www.mdanderson.org

http://www.insidecancer.org/

http://www.mdanderson.org/



Second Year: Semester III- Core Theory [lab cum theory]

Course Number


18 PBT15CT205 Environmental Biotechnology

2 2 3 60


To impart knowledge on concepts of ecosystem, environmental pollution and management by biotechnology

On successful completion of the course, the student will be able to comprehend the basic concepts of ecology and microbial association and functions

18. Environmental Biotechnology

Unit 1 Basic Ecological Concepts and Principles: Ecosystem: types, trophic levels: structure, Ecological succession, food chain, food web, energy transfer in ecosystem, Homeostasis, population and community dynamics, ecological efficiency.

Unit 2 Environmental Pollution: Types and sources- air, water- Eutrophication and soil- Methods for the measurement of pollution and control strategies. Methodology of environmental management – the problem solving approach, its limitations. Global environmental problems: Ozone depletion, UV-B, green house effect and acid rain, their impact and approaches for management. Environmental safety guidelines

Unit 3 Microbiology of wastewater treatments: Aerobic process: Activated sludge trickling filter and oxidation ponds) Anaerobic processes: Anaerobic digestion Examples of Industrial of wastewater treatments from tannery, dairy, distilleries and antibiotic industries; Biosurfactant usage in treatment of crude oil wastes

Unit 4 Microbial degradation of aromatic hydrocarbons: role of oxygenases in microbial metabolism through ortho and meta cleavage pathways, degradation of xenobiotics. Denitrification: Physiology of denitrifying bacteria Microbial degradation of aliphatic hydrocarbons: metabolic pathways and degradation Biofuels: Biomethane as natural fuel, Reactor configurations, Special factors for the design of anaerobic sludge digesters, microbial groups involved in biogas production and interactions among them. Feed stock, uses of spent slurry

Unit 5 Microbes in extreme environment: Acidophiles, thermophiles and halophiles: Types, physiological adaptations and biotechnological implications Marine microbiology: Microbial communities and Interactions: Beneficial and antagonistic, population and community dynamics, polysaccharides, biofilms and biofouling.

Lab Program Unit 6:

1. Enumeration of microorganisms from air samples 2. Air samplers and analysis of air samples 3. Enumeration of E. coli population in different water samples by MPN method 4. Estimation of Dissolved Oxygen (DO) in various water samples

Unit 7 5. Detection of azo dye (methyl orange/ Congo red/ methyl red/ trypan blue) decolorization using bacteria from water samples

6. Calculation of emulsification index of different hydrocarbons by biosemulsifier producing bacteria

7. Detection of glycolipid biosurfactants from bacteria using Blue Agar Plate method 8. Visit to pollution control board field laboratories

Text Book: 1. Textbook for Environmental Sciences, by Erach Bharucha for University Grants Commission

Curriculum, 2004 2. Biotechnology: Environmental Processes I, Volume 11a, Second Edition Editor(s): H.-J. Rehm,

G. Reed, 2008, Wiley-VCH-GmbH, ISBN: 9783527283217\ References:

1. Comprehensive Biotechnology, vol. 4, M.Moo-young (Ed- in – Chief), Pergamon Press, Oxford, 2

nd edition, ISBN: 978-0-444-53352-4, 2011

2. Environmental Microbiology (Second Edition), Edited by:Ian L. Pepper, Charles P. Gerba, Terry Gentry and Raina M. Maier , ISBN: 978-0-12-370519-8, Elsevier Inc publishers, 2009



3. Techniques in microbial ecology. Methods in microbiology, by Girgorova, R., and J.R. Norris (eds) 1990 Academic Press, London.

Online Resources: 1. http://earthobservatory.nasa.gov/GlobalMaps/view.php?d1=MOD17A2_M_PSN 2. Molecules 2009, 14, 2535-2554; Bacterial Extracellular Polysaccharides Involved in Biofilm

formation

19. Genetic Engineering

UNIT 1 Introduction: Milestones in genetic engineering; steps involved in cloning; cloning strategies (T/A cloning, directional cloning); DNA delivery techniques Cloning and Vectors: Features and types of vectors (plasmid, phage, plant and animal, expression vectors); Linkers and Adaptors

UNIT 2 DNA Modifying Enzymes: DNA modification enzymes, DNA polymerase; topoisomerase; phosphatase; kinase; reverse transcriptase; ligases; methylases; restriction mapping

UNIT 3 Screening of clones by amplification techniques: Amplification techniques : Polymerase chain reaction (PCR) – types of PCR, components of PCR, primer designing, qPCR, RCA, LAMP

UNIT 4 Screening of clones by hybridization techniques: Southern, northern, western, Microarray (DNA, protein, tissue); Screening based on cloned Proteins , Nucleic acid, and Immunology, marker genes, reporter genes

UNIT 5 Gene library construction and analysis: Artifical chromosome, Library preparation – gDNA and cDNA; Sequencing techniques : Sanger‟s dideoxy method, automated method, NGS, third generation sequencing, whole genome sequencing, exome analysis

UNIT 6 Protein expression and analysis: Site directed mutagenesis, protein engineering, codon bias, protein folding, purification of recombinant protein; Identification and annotation of genes, identification of open reading frames (ORF), Bioinfo Tools available for heterologous protein expression

UNIT 7 rDNA applications: Application of small RNA‟s (mi, si), RNAi, antisense technology, Zinc finger nuclease, Ribozymes, Aptamers, in vitro transcription and translation, DNA vaccines, therapeutic proteins , edible vaccines, GM crops (insect resistance – BT, viral resistance – TMV;CaMV or papaya), yeast two hybrid systems.

Text Books: 1. Genetic engineering. An introduction to gene analysis and exploitation in eukaryotes,

S.M.Kingsman and A.J. Kingsman, Blackwell Scientific Publications, Oxford, 1998. 2. Molecular Biotechnology – Glick Pasternak 3. DNA cloning: a practical approach, D.M. Glover and B.D.Hames, IRL press, Oxford, 1995.

Reference Books: 1. Molecular cloning: A laboratory manual J. Sambrook, E.F. Frisch and T. Maniatis, Cold Spring

Harbor Laboratory Press, New York 2000. 2. Molecular and Cellular methods in Biology and Medicine, P.B. Kaufman, W.Wu.D.Kim and

L.J.Cseke, CRC Press. Florida.1995 Online Resources: http://nptel.ac.in/ Molecular Biology Protocols

Second Year: Semester III ; Category: Core theory

Course Number


19 PBT15CT207 Genetic Engineering 3 3 45 Objectives Learning outcomes

To understand the principles of genetic cloning and the applications of rDNA technology

On successful completion of the course, the student will be able to –

comprehend the cloning principles and strategies

analysis of the clones

http://earthobservatory.nasa.gov/GlobalMaps/view.php?d1=MOD17A2_M_PSN

http://nptel.ac.in/



Second Year: Semester III; Category: Discipline Specific Elective (DE) Course

Course Number


20 PBT15DE209 [BDE 004]

Pharmaceutical Biotechnology

3 0 0 3 45


To provide knowledge on the biotechnology aspects of therapeutic agents.

The student would understand the biotechnological approaches to therapy Understand the principles of the new

biotechnology based assays the therapeutic uses of plant products

20. Pharmaceutical Biotechnology

UNIT 1

Introduction to Pharmaceutical Biotechnology: Overview of products, classification of pharmacologic agents based on chemistry and source. Phytopharmaceuticals: Screening tests for phyto constituents – alkaloids, and terpeniods. Three examples of commercial natural products from marine and terrestrial organisms.

UNIT 2

Pharmaceutical assays for activity in vitro assay systems based on enzymes and antimicrobial growth inhibition assays, in vivo assays based on Cell lines; tissues, organ, animal models; , transgenic animal- diabetes; hepatitis

UNIT 3 Vaccines: Vaccine design and production, classification, genetically recombinant vaccines, Advantages & disadvantages – Examples – Hepatitis B vaccines, Cholera Vaccines.

UNIT 4 Immunology Antisera– hyper immune gamma globulin – monoclonal antibodies – uses.

UNIT 5 Novel Pharmaceutical Agents Genetherapy, Recombinant proteins, Probiotics and neutraceuticals.

UNIT 6 Economic and Legal Considerations in Pharmaceutical Biotechnology FDA guidelines- preclinical trials, acute, sub acute, chronic and teratogenic studies. Clinical trials- Phases I, II and III, ICMR guidelines for design and conduct of clinical trials, licensing, and drug control.

Text Books: 1. Pharmaceutical Biotechnology. 2009. Tripathi N & Sharma M. Commonwealth

Publishers.

2. Quality control in Herbal drugs- An approach to evaluation of botanicals, 2002. P.K. Mukherjee, Business Horizons.

References Books: 1. Phytochemical methods- A guide to modern techniques of plant analysis, J B Harborne; 1998, 3

rd

Ed., Chapman & Hall. 2. Natural Products Isolation, R.J.P. Cannell; 1998, Ed., Humana Press.

3. Molecular Biotechnology: Principles and applications of recombinant DNA. 2009. 4th

Edition. Glick BR, Pasternak JJ & Patten CL. ASM Press.

Online Resources:

www.uic.edu/depts/cphb/CPB/Welcome.html

http://www.cnbc.com/id/10000904

http://www.eapb.org/

http://www.uic.edu/depts/cphb/CPB/Welcome.html

http://www.cnbc.com/id/10000904

http://www.eapb.org/



Second Year: Semester III- Course Type: Ability Enhancement Compulsory Course (AE)

Course Number

Course Code Title L T P C Hours

21 PBT15AE211 Scientific Writing and Research Methodology

4 2 60

Objectives Learning Outcome

This course would initiate the researchers and students to learn the techniques and methodologies needed for scientific communication

On completion of this course the student will be able to –

Understand the different types or formats of

Scientific communications

Practice and learn through case studies the right

form of communication

Understand the need for ethics in conduct of

research program

Design a project proposal

Develop a manuscript for publication

Grant writing proposals

The CIA Evaluation of this AE course is in the form of a pre- proposal of a chosen research project and

outlined in Table 17.2. 21. Scientific Writing and Research Methodology

UNIT 1

Research methodology and Literature Collection: General outlines of Selection of a research problem. General outlines of design of a research project. Types of literature: reviews, abstracts, short notes, journal articles, magazines, periodicals, books and proceedings. Sources of literature – INSDOC, MEDLINE, Chemical & Biological abstracts, Current Contents, Google Scholar; Research GATE; NCBI; Patents; Pubmed, Human Genome Centers, The Genome Database, Online Mendelian Inheritance of Man (OMIM) and Online Journals; Patents. Collating and Organizing Information – Using Reference managers: Entrez; Mendeley; EndNote

Practicals: Use of reference managers. UNIT 2 Principles of writing; Design of a research project – Fundamentals of writing a proposal;

thesis writing, case studies; worksheets on effective writing; Proposals for funding. Preparation of manuscript: Components of a manuscript; Presentation of data, bibliography, Citations, Sources of information.

UNIT 3 Basics of experimental designs – Experimental Strategy Maintaining a record / Log book: Recording experimental research; Recording data, tabulations and data entry. Qualitative analysis of data. Types of data – Distinctions between quantitative and qualitative studies Qualitative analysis of data. Recording experimental research; Recording data, tabulations and data entry.

UNIT 4 Submission of research proposal: Students are taught how to find the gaps in the knowledge from library studies. Then generate a hypothesis. „How to design experiments to prove or disprove a hypothesis‟. They will also be asked to do a literature survey, library studies and prepare a work outline (research proposal) in consultation with the faculty. Those students seeking to work with engineered organisms/ human volunteers/ animals must seek the clearance of the appropriate committees at the end of this semester to facilitate an easy start on their final semester projects.

UNIT 5 Familiarizing with principles of ethics in research; Institutional Committees for ethics; types, Functions and forms – ICMR; CPCSEA; IBSC; CITI; informed Consent – types and what it should contain Practical: forms; what is essential; principles for filing a form; informed Consent; check lists and disclosures

References and Online Resources: Successful Scientific Writing by Mathews JR and RW Mathews. 2008. Cambridge University Press. Guide to Scientific Writing. Clinical Chemistry 56:4 521–524 (2010) by Thomas M. Annesley.



Text Books:

1. Genetic engineering. An introduction to gene analysis and exploitation in eukaryotes, S.M.Kingsman and A.J. Kingsman, Blackwell Scientific Publications, Oxford, 1998. 2. Molecular Biotechnology – Glick Pasternak 3. DNA cloning: a practical approach, D.M. Glover and B.D.Hames, IRL press, Oxford, 1995.

Reference Books: 1. Molecular cloning: A laboratory manual J. Sambrook, E.F. Frisch and T. Maniatis, Cold Spring

Harbor Laboratory Press, New York 2000. 2. Methods in enzymology vol.152, Guide to Molecular cloning techniques, S.L. Berger and A.R.

Kimmel Academic press, Inc. San Diego, 1998. 3. Methods in enzymology vol 185, gene expression technology, D.V.Goeddel Academic Press, Inc.

San Diego, 1990. Online Resources:

1. https://www.addgene.org/plasmid-protocols/PCR-cloning/ 2. Standard DNA Cloning. Bio-protocol Bio101: e52. http://www.bio-protocol.org/e52 (2011).

Second Year: Semester III – Core Lab

Course Number

Course Code Title L T P C Hours

23 PBT15CL253 Cancer Biology and Immunotechnology (Practicals)

4 4 120

Objectives Learning Outcomes

To learn various cells normal and cancerous cells and their shed substances cancer development and progression Understand basics of immunology and various techniques to study antigen-antibody relationships

On successful completion of the course, the student will be able to – understand various types of cancer, tumor invasion, markers in cancer research and diagnosis. Demonstrate antigen-antibody relationships and their detection methods.

Second Year – Semester – III; Category: Core Lab

Course Number


22 PBT15CL251 Genetic Engineering Techniques (Practicals)

8 4 120


To impart skills on laboratory techniques in molecular cloning

On successful completion of the course, the student will be able to –

comprehend the skills required to do experimental cloning

set up experiments using advanced tools of selecting vectors for cloning; sequencing an analysis, PCR, expression of cloned products.

No. 22. Genetic Engineering Techniques (Laboratory Course)

1 Genomic DNA isolation from bacteria 2 Genomic DNA isolation from eukaryote (blood/plant) 3 Plasmid DNA isolation from transformed E. coli by alkaline lysis method 4 Cloning by PCR a) Gene Isolation by amplification of specific gene using gene specific primers by gradient

Polymerase chain reaction (PCR) b) Purification of PCR products by column purification c) Ligation of PCR product with T vector d) Competent cell preparation and transformation with a standard vector pTZ57RT e) Screening of recombinants by blue/white method (insertional inactivation of lacZ gene) f) Screening transformants using colony PCR 5 Southern blotting / hybridization – gDNA or rClone 6 Affinity purification of rGST protein

https://www.addgene.org/plasmid-protocols/PCR-cloning/



23. Cancer Biology and Immunotechnology (Practicals) No. Experiment Titles 1. Estimation of serum sialic acid i.e. protein bound and lipid bound 2. Estimation of tissue sialic acid i.e. lipid bound 3. ELISA studies for tumor markers-β.HCG 4. Histopathological diagnosis of cancer and staging of the disease.-demonstration 5. Tissue processing and staining and types of cancer:

Chronic myeloid leukemia, Adenoma and Adenocarcinoma; Leiomyoma Squamous cell carcinoma.

6. Immunophenotyping- Visit to Pathology (Demonstration) 7. Cancer Cell line maintenance – Vero or HeLa or Hep2 and cell counting 8. Isolation of genomic DNA from cancer cell lines and DNA fragmentation Assay 9. Enumeration of WBC & Differential Leukocyte Count. 10. Double Immuno Diffusion 11. Counter Immuno Electrophoresis 12. Blood grouping & Rh typing 13. Widal test (Slide and Tube agglutination) 14. Preparation of lymphocytes and identification of T lymphocytes 15. Preparation of Blood products – visit to Blood Bank 16. ImmPort: Bioinformatics Immunology Databases

References:

1. Practical immunology 4th edition, Frank C. Hay, Olwyn M.R. Westwood, 2002. 2. A Handbook of Practical. Immunology edited by G. P. Talwar, Vikas Publishing. House, New

Delhi, 1983. 3. Practical Immunology 3rd edition. Oxford, Blackwell Scientific publications. Hudson L, Hay, 1999.

Dissertation and Viva -Voce DISSERTATION:

Students are taught how to find the gaps in the knowledge from library studies. Then generate a

hypothesis; how to design experiments to prove or disprove a hypothesis. How to test a null hypothesis;

requirements of a good experimental design; use of instruments for research, pH-meter, colorimeter,

spectrophotometer, centrifuge, radioactivity counters, fraction collector, peristaltic pumps, electrophoresis

equipments and other advanced equipment like GLC, HPLC, PCR apparatus etc.

Within the framework and facilities of the Department of Biotechnology and other available infrastructure

of the university, the students will be assigned a dissertation research project. They will also be asked to

do a literature survey, library studies and prepare a work outline (research proposal) in consultation with

the faculty. Maintenance of a lab notebook with authenticated record of work carried out will also form a

part of the formative assessment.

The faculty guide, by a periodical consultation with the HOD will focus the work of the student. Then the

student will spend roughly 90 working days in the fourth- semester on the allotted project. The student

will be continuously assessed and graded by the faculty and Professor on their attendance, devotion to

work, data gathering, honest and authentic lab work; documentation, and interpretation and in the overall

preparation of final dissertation work report. The dissertation work will thus constitute an important

Second Year – Semester – IV: Category: Core Project

Course Number


25 PBT15RP252 Dissertation and Viva -Voce

- - 45 18 540


To understand the methods to do select a topic for research, conduct research, communicate effectively and log the activity as per international guidelines

On successful completion of the course, the student will be able to:

Learn how to collect, read and manage research information

Plan experiments, conduct and observe results

Write and publish results effectively



objective proof of the knowledge and academic / work experience gained by them in the M Sc -

Biotechnology course.

The research project work could be industry based or laboratory based. Permission maybe granted to

select number of students to carry-out a part of their lab work in external institutions by the HoD based on

the needs assessment and scientific depth of the research and as part of collaborations.

The summative evaluation of the project would be done by university examination on the basis of content

and output of the submitted dissertation; and dissertation viva voce before the examiners. Dissertation is

a bound form of a detailed report of the research carried out by each student. It has to be presented in

the internationally accepted format containing a title sheet, bonafide certificate, contents, introduction,

review of literature, scope of the research including lacunae; objectives or hypothesis, experimental and

results, discussion and summary, references and acknowledgement. The model format of layout,

designing, order of binding; referencing style are available on our website (mscbiotech.net) and maybe

followed. The framework for evaluation of formative and summative assessments is provided in the,

table 17.2 and scheme of examinations table 26. A passing minimum of 50% in the continuous

assessment is essential to qualify for appearing in the end semester with ≥80% attendance.

It is desirable that the student be encouraged to submit one publication or presentation from out of the

thesis before appearing for the university examinations. This will be culmination of the three semesters

of research orientation of the students which is an asset to any organization employing them.

Profile and Syllabi for Elective Courses LIST OF Discipline Specific Elective (DE) Courses Offered for M Sc Biotechnology Program

Dept. of Biotechnology

S. No.

Code Number

Course Title Credit Semester

Level

Course Code Current Status

1. BDE001 Basic genetics 3 i PBT15DE107 Applicable for 2015 batch only

2 BDE002 Molecular Genetics

3 i PBT15DE107 Applicable for 2015 batch only

BDE002 Molecular Genetics

3 iv PBT15DE202 Applicable for 2016 batch onwards

3 BDE003 Advanced Microbiology

3 i PBT15DE107 Applicable for 2015 batch only

4 BDE004 Pharmaceutical Biotechnology

3 iii PBT15DE209 Applicable for 2015 & 2016 batch onwards. No choice

5 BDE005 Biology of Viruses 3 iv PBT15DE202 Applicable for 2016 batch onwards

6 BDE006 Disorders of Metabolism


7 BDE007 Molecular Diagnostics


8 [BGE023] Bioinformatics

3 ii PBT15DE110 Applicable for 2015 batch & 2016 batch onwards No choice



Department of Biotechnology

Discipline Specific Elective (DE) Course - Curriculum Scheme

Co

urs

e

Nu

mb

er

Course Code

Cate

go

ry

Dis

cip

lin

e

Sp

ecif

ic

Ele

cti

ve (

DE

) C

ou

rse

Course Title Credits/Week Hours / Semester

Faculty

Code

Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical

train

ing (

CT

) P

ractical

(P)/

Researc

h P

roje

ct

Cre

dit

s (

C)

Lect. / T

ut.

Pra

ctical

To

tal H

ou

rs

1. BDE001 DE Basic genetics BMS 3 3 45 45

2. BDE002 DE Molecular Genetics BMS 3 3 45 45

3 BDE003 DE Advanced Microbiology BMS 3 3 45 45

4 BDE004 DE Pharmaceutical Biotechnology (in Syllabus)

BMS 3 3 45 45

5 BDE005 DE Biology of Viruses BMS 3 3 45 45

6 BDE006 DE Disorders of Metabolism

BMS 3 3 45

7 BDE007 Molecular Diagnostics BMS 3 3 45 45

Generic Elective (GE)

1 BGE026 GE Nanodiagnostics BMS 3 3 45 45

2 BGE027 GE Health care Biotechnology

BMS 3 3 45 45

3 BGE029 GE Plant Tissue Culture Technology

BMS 3 3 45 45

4 BGE030 GE Marine Biotechnology BMS 3 3 45 45

5 BGE031 GE Antimicrobial Agents BMS 3 3 45 45

6 BGE032 GE Algal Biotechnology BMS 3 3 45 45

7 BGE036 GE Nanotechnology BMS 3 3 45 45

8 BGE037 GE Alternative models for Experimental Toxicology

BMS 3 3 45 45

First Year – Semester – I; Category: Elective General

Course Number Course Code Course Title L T P C Total Hours

2 [BDE002] Molecular Genetics 3 3 45


To impart knowledge on 1. Genes and chromosome 2. Recombination. Plasmids and its application


Gene concept and organization and their implications

Plasmid and its types

4. Molecular Genetics

UNIT 1 Genes and Chromosomes: Gene concept; genome organization; complexity of eukaryotic genome; Chromosome structure and organization; chromosome numbers

UNIT 2 Mutation and Repair: Types of mutation; mutagens and carcinogens: types of repair - excision repair, SOS repair, photoreactivation.

UNIT 3 Recombination: Homologous recombination, non-homologous recombination: FLP/FRT and Cre/Lox recombination; holliday junction, Rec protein, Recombinases.

UNIT 4 Jumping genes: Transposons and Retroposons: An Introduction with examples: insertional sequences (IS) and Complex transposons - Tn9 and Tn3. AC/DS elements, Retroposons – evolution and mechanism of retropostion

UNIT 5 Biology of Plasmids: Types of plasmids, incompatibility grouping, copy number replication of col E1 and F plasmids; Natural and artificial methods of plasmid transfer, their significance and applications.

UNIT 6 Evolution: Genetic diversity and evolution; genotyping, SNP analysis; variation analysis; phylogenetic analysis and bioinformatics tools



Text Books 1. Biochemistry & Molecular biology, William H Elliott and D.C.Elliot, Oxford University press. 2000 2. Microbial Genetics by S.R.Maloy. J.E.Cronon, D.Freifelder 1996 Jones & Bartlet Publ. 3. Principles of Genetics. 8th edition by Gardner AG 2002.

Reference Books 1. Genetics: analysis and principles, 4 edition, Robert Brooker. McGraw-Hill, 2011. 2. Genetics: From Genes to Genomes, 4 edition, Leland Hartwell, Leroy Hood, Michael Goldberg,

Ann Reynolds, Lee Silver, McGraw Hill. 2010. 3. Molecular Biology of Genes. 4th edition by Watson, Hopkins, Roberts, Steitz, Weiner. 2004.

ONLINE RESOURCES: http://star.mit.edu/genetics/tutorial/index.html nptel.nic.in

Semester – Even; Category: Discipline Specific Elective (DE) Course

Course Number

Course Code

Course Title L T P C Total Hours

5 BDE005 Biology of Viruses 3 0 3 45


To provide basic principles in virology

The student have understood the basics of virology; understood how they are isolated and studied; molecular aspects of viruses infecting bacteria and plants.

Biology of Viruses

UNIT 1 General properties of Viruses, Viroids, prions, satellite viruses, morphology and structure of viruses, classification of viruses, transmission of viruses

UNIT 2 Isolation and purification of viruses, in vitro cultivation; and in- planta cultivation methods; Assay of viruses and their quantification

UNIT 3 Bacteriophages – Molecular structure of M13, phi X174, Lambda viruses and their applications. Vectors derived from bacterial viruses and their applications

UNIT 4 Plant Viruses – classification, transmission of viruses and vectors, biology of type viruses – Infection, symptoms, Plant viruses as useful tools Genome organization & replication: (+) RNA viruses e.g.TMV; Potato virus X; Cucumber mosaic virus

UNIT 5 Genome organization & replication: dsRNA viruses, (-) and ambisense RNA viruses- Tospo viruses - Tomato spotted wilt virus.

UNIT 6 Genome organization & replication : DNA viruses e.g., CaMV; Geminiviruses; Subviral agents

Text Books: 1. Virology – A. Cohen (2000) 2. Viral & Rickettsial Infections of Man – Horsfall & Tom, 1998. 3. Cancer biology & management – An introduction: C.J.Williams & John Wiley 1990. 4. Basis of disease, 7

th ed..Vinay Kumar, Abul K Abbas, N Fausto

Reference Books: 1. Fields Virology, 4

th ed. Eds, D M Knipe, PM Howley. Lippincott Williams and Wilkins.

Online Resources: www.freebookcentre.net www.txbiomed.org/departments/virology www.ictvonline.org

Semester – Even; Category: Discipline Specific Elective


6 [BDE006] Disorders of Metabolism 3 3 45


To provide new knowledge concerning diseases of various intermediary metabolic pathways

On successful completion of the course, the student will be able to - understand disorders of the various metabolic pathways

http://www.ictvonline.org/



DISORDERS OF METABOLISM Unit 1 Introduction: to metabolic pathways in animals and humans, inborn errors of metabolism,

diagnosis of metabolic disorders Unit 2

Vitamin metabolism: Vitamin Deficiencies of lipid soluble and water soluble vitamins, Vitamin-A&D and Vitamin-B7 and B-12 disorders

Unit 3 Protein metabolism: Outline of Biosynthesis of amino acids, urea cycle, Disorders of protein metabolism: Phenylketonuria, Alkaptonuria, albinism

Unit 4 Carbohydrate & Lipid metabolism: Glycolysis, TCA cycle, Glycogenesis and Glycogenolysis. Disorders of carbohydrate metabolism:- Glycogen storage diseases, Diabetes Mellitus. Oxidation and Synthesis of Fatty acids. Lipid storage disease:- Nieman-pick disease, Tay-Sachs disease and Atherosclerosis.

Unit 5 Nucleic acid metabolism: Biosynthesis of purine and pyrimidine nucleotides, Catabolism of nucleotides, Disorders of Nucleic acid metabolism: Hyperuricemia, Gout, Xeroderma Pigmentosum nucleic acid based diagnostic methods

Unit 6 Bioinformatics - Identification of SNP‟s using bioinformatic tools for Disorders of Aminoacid metabolism-Alkaptonuria, Gout, Atherosclerosis and Diabetes.

Text Books 1. Fundamentals of Biochemistry: Life at the MolecularLevel 2012 by Donald Voet, Judith G. Voet,

Charlotte W. Pratt Wiley; 4th Edition Binder Ready Version edition ISBN-13: 978-1118129180 2. Lehninger Principles of Biochemistry Hardcover,Edition: 5th, 2008, by David L. Nelson, Michael

M. Cox , ISBN-13: 978-0716771081 3. Harpers Illustrated Biochemistry 30th Edition 2015 by Victor W. Rodwell, David Bender

Reference Books

4. Practical Clinical Biochemistry by Harold Varley. Publisher: CBS; 6th edition, 2006. ISBN-10:

8123904274 5. Biochemistry Hardcover by Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, W.H.Freeman &

Co Ltd; 5th Revised edition, 2002 ISBN-13: 978-0716746843 Online Resources:

NPTEL.nic.in

NCBI

Semester – Even; Category: Discipline Specific Elective (DSE) Course


7 [BDE007] Molecular Diagnostics 3 3 45


To impart knowledge principles of molecular diagnosis


modern tools in diagnosis

the wide range of techniques used

Molecular Diagnostics

UNIT 1 PCR and its applications: Principle of PCR, components of PCR, types of PCR - qPCR, RT-PCR, multiplex PCR; primer design; application of PCR, and qPCR

UNIT 2 Amplification methods: RCA - principle and methodology; LAMP PCR - principle; Marker assisted analysis - RAPD, AFLP, RFLP; Amplification refractory mutation systems (ARMS); Ligation chain reaction based diagnostics for detection of infectious and genetic diseases.

UNIT 3 Hybridization and in situ hybridization: Principle of hybridization; types of hybridization - Southern, Northen, Colony, Plaque, Dot/ Slot blot; Methodology and applications; in situ hybridization - principle and types (FISH, mFISH, on-FISH); Array based diagnostics - Microarray and tissue microarray

UNIT 4 Probe design and detection: Probe; types of probes (DNA, RNA and protein - antibody); design of probe; features of probe; labeling of probe (Random labeling, nick translation, end labeling); labels - biotin, DIG, Radiolable, fluorophore; Detection of probe - autoradiography, phosphorimager

UNIT 5 Molecular Beacons: Molecular beacons; quenchers; BRET; FRET; Fluorophores; chemiluminescence, phosphorescence, bioluminescence and applications



UNIT 6 Nanodiagnostics: Colorimetric detection of NA using NPs; Lateral flow method; lab-on-chip (biochip); Cantilevers as Biosensors for Molecular Diagnostics –Carbon Nanotube Biosensors -FRET-Based DNA Nanosensors. Ion Channel Switch Biosensor Technology -Electronic Nanobiosensors -Electrochemical Nanobiosensors -Quartz Nanobalance Biosensors -Viral Nanosensors –PEBBLE Nanosensors -Microneedle-Mounted Biosensors Optical Biosensors- Nanowire (NW) Biosensors -Nanoscale Erasable Biodetectors

Text Books 1. Medical Biomethods handbook by John M.Walker & Ralph Rapley, Humana Press. 2. Tietz Textbook of Clinical Chemistry and Molecular Diagnosis by Carl A. Burtis, Edward R. Ashwood, David E.Bruns – Elsevier 3. Balley & Scott‟s Diagnostic Microbiology by B.A.Forbes, Daniel F.S and Alcie S.W.-Mosby Elsevier Reference Books 1. Essentials of Diagnostic Microbiology by Lisa Anne Shimeld, Delmer publishers Int. Thomson Publishing Co. 2. Molecular Diagnostic PCR Handbook by Gerrit J. Viljoen, L. H. Nel, J. R. Crowther – 2005 3. Molecular Diagnostics: Current Technology and Applications by Juluri R. Rao, Colin Craig Fleming, John Edmund Moore – 2006 4. Molecular Diagnostics: A Training and Study Guide by Gregory J. Tsongalis, William B. Coleman - 2002

Online Resources:

1. http://advameddx.org/

2. http://jmd.amjpathol.org/ 3. http://www.amp.org

Nanodiagnostics

UNIT 1 Nanoparticles and Diagnostics: Introduction to nanodiagnostics (need for nanoparticles), Gold nanoparticles and detection of macromolecules (protein, nucleic acids). Quantum dots and Magnetic nanoparticles and their application in molecular detection. Nanowires and CNT and their applications

Dept. of Biotechnology Generic Elective (GE) Course

S. No.

Code Number

Course Name Credit Semester

Odd / Even

Faculty Department Level UG/PG/I/D/Ph.

D.

Semester

Level

Generic Elective (GE)

1 BGE026 Nanodiagnostics 3 EVEN BMS Biotechnology PG 2

2 BGE027

Health care Biotechnology

3 EVEN BMS Biotechnology UG 2

3 BGE029

Plant Tissue Culture Technology

3 EVEN BMS Biotechnology UG 2

4 BGE030

Marine Biotechnology

3 ODD BMS Biotechnology UG 1

5 BGE031 Antimicrobial Agents 3 ODD BMS Biotechnology PG 1

6 BGE032 Algal Biotechnology 3 ODD BMS Biotechnology UG 1

7 BGE036 Nanotechnology 3 ODD BMS Biotechnology UG 3

8 BGE037 Alternative models for Experimental Toxicology

3 EVEN BMS Biotechnology PG 2

Semester – Even; Category: Generic Elective (GE) Course - PG

Course Number


BGE 026 Nanodiagnostics 3 3 45 Objectives Learning outcomes

To impart knowledge on nanomaterials and their applications


comprehend the types and features of nanomaterials

Applications of nanoparticles with macromolecules



UNIT 2 Nanopores and crystals: Use of Nanocrystals in Immunohistochemistry -Imaging Applications of Nanoparticles Study of Chromosomes by Atomic Force Microscopy-Applications of Nanopore Technology for Molecular Diagnostics DNA–Protein and DNA–Nanoparticle Conjugates, Single nanopore for DNA sequencing.

UNIT 3 Protein based Nanotechnologies: Nanoarrays - NanoProTM System -Nanofluidic/Nanoarray; ProteinNanoarrays -Fullerene Photodetectors for Chemiluminescence Detection on Microfluidic Chips -Protein Microarray for Detection of Molecules with Nanoparticles; Protein Nanobiochip; Protease-Activated QuantumDot Probes - Single-Molecule Detection

UNIT 4 Nucleic Acid based Nanotechnologies: Devices to Detect a Single Molecule of DNA-Self-Assembling; nanoprinting of DNA, RNA, Nucleic acid chips; lab on a chip (LOC), Lateral flow devices for on filed detection (Point-of-Care Diagnostics), Colorimetric detection of NA using NPs,

UNIT 5 Nanobarcodes and imaging: Nanobarcodes Technology -Nanobarcode Particle Technology for SNP Genotyping -Qdot Nanobarcode for Multiplexed Gene Expression Profiling -BiobarcodeAssay for Proteins; Single-Molecule Barcoding System for DNA Analysis; Mammalian (Myosin family) Nanobiosensors: Science of Self-assembly - From Natural to Artificial Structures Nanoparticles in Biological Labeling and Cellular Imaging.

UNIT 6 Biosensors: Cantilevers as Biosensors for Molecular Diagnostics –Carbon Nanotube Biosensors -FRET-Based DNA Nanosensors. Ion Channel Switch Biosensor Technology -Electronic Nanobiosensors -Electrochemical Nanobiosensors -Quartz Nanobalance Biosensors -Viral Nanosensors –PEBBLE Nanosensors -Microneedle-Mounted Biosensors Optical Biosensors- Nanowire (NW) Biosensors -Nanoscale Erasable Biodetectors

Text Books:

1. Biological molecules in Nanotechnology by Stephen Lee and Lynn M Savage 2. Nanotechnology – Basic Science & Emerging Technologies by Chapman & Hall/CRC 2002. 3. Nanotechnology by Gregory Timp (Ed), Spring 1998.

Reference Books: 1. The Handbook of Nanomedicine Kewal K. Jain, Humana Press, (2008). 2. Nanomedicine: A Systems Engineering Approach” 1st Ed., Zhang,. Pan Stanford Publishing, (2005). 3. Nanotechnology – A gentle Introduction to the Next Big Idea by Mark Ratner and Daniel Ratner, Pearson Education, 2005.

Online Resources: http://www.iinano.org/research http://www.nanodiainc.com/

Semester – Even; Category: Generic Elective (GE) Course - UG


BGE 027 Health care Biotechnology 3 3 45


To impart knowledge on novel strategies for treatment of diseases.

Understand and appreciate the applications of biotechnology

Explain the types of novel therapeutic agents

Understanding novel pharmaceutical agents

Defining new treatment modalities available

Health care Biotechnology

UNIT 1 Peptides, Oligosaccharides, Gene therapy: Overview – Introduction to endogenous peptide, proteins & modifications. Oligosaccharide synthesis, heparin, Glycoproteins, Polysaccharide bacterial vaccines, Approaches to carbohydrate based cancer vaccines, Gene therapy, Antisense therapy, Ribozyme.

UNIT 2 Cardiovascular Drugs: Myocardial Infarction agents, Endogenous vasoactive peptides, Hematopoietic agents. Anticoagulants, antithrombotics and homeostasis

UNIT 3 Drugs for Endocrine Disorders: hormones and analogs - Diabetes Mellitus, Breast Cancer, Hypothyroidism, Hyperthyroidism, Pituitary drugs, Topical corticosteroids, Agents affecting the immune response



UNIT 4 Organ Specific Chemotherapeutic Agents: Basic concepts and novel advances, Brain-specific drug targeting strategies. Pulmonary drug delivery.

UNIT 5 Targeted drugs: Synthesis of antibacterial agents - serine protease, antifungal, antiprotozoal, Antihelminthic agents, Antiamoebic agents, Antiviral agents. Radiological Agents: Radiosensitizers and Radioprotective agents

UNIT-6 Cosmetics & other consumer products: Proteins, Peptides, Enzymes and Their Applications in Personal Care, Biotechnology in Skin Care, anti-aging, Anti-malarial insecticide

Text / Reference Books:

1. Pharmaceutical Chemistry by Cristine M. Bladon. John Wiley & Sons. Ltd. (2002)

2. Burgerís Medicinal Chemistry and Drug Discovery (5th edition) by Manfred E.Wolf . A Wiley &

Sons.Inc. (2000).

3. Drug Targeting Organ- Specific Strategies by Grietje Molema and Dirk K.F.Meijer. Wiley -VCH.

(2002)

4. Biotechnology in Personal Care (Cosmetic Science and Technology) by Raj Lad (Editor), CRC

Press; 1 edition (March 6, 2006)

Semester – Even; Category – Generic Elective (GE) Course - UG

Course Number


BGE 029 Plant Tissue Culture Technology

Lab cum theory

1 0 4 3 75


To provide training in plant tissue culture techniques

The student would have become proficient in aseptic techniques; initiate and establish plant cell cultures.

Theory: Plant tissue culture Technology UNIT 1

Introduction to cell and tissue culture, Tissue culture media and aseptic techniques. Initiation and maintenance of callus and suspension cultures. Protoplast isolation, culture and fusion: Selection of hybrid cells and regeneration of hybrid plants: symmetric and asymmetric hybrids, cybrids.

UNIT 2

Embryo culture and embryo rescue, Anther, pollen and ovary culture for production of haploid plants and homozygous lines. Cryopreservation, slow growth for germplasm conservation. Liquid Cultures of Plant Cells: Initiation and maintenance of callus and suspension cultures; Bioreactors and their applications.

UNIT 3

Plant transformation technology: Outline of transformation technology. Vectors and methods for gene transfer in plants. Markers and reporters used for plant transformation. Applications of transgenic plant technology: insect resistance (Bt genes), Biopharming-Therapeutic proteins in transgenic plants

Laboratory exercises

UNIT 4-6 1. Preparation of media 2. Initiation and Organ culture 3. Callus induction and propagation 4. Protoplast Isolation 5. DNA isolation from plant tissues 6. Encapsulation of cells/ tissues

Text Books: 1. Plant Tissue Culture: An Introductory Text by Bhojwani, Sant Saran, Dantu, Prem Kumar, 2013;

Springer India; eBook ISBN; 978-81-322-1026-9

2. Plant Cell Culture Protocols, Methods in Molecular Biology, Volume 877 2012 by Editors: Víctor

M. Loyola-Vargas, Neftalí Ochoa-Alejo 2012; DOI - 10.1007/978-1-61779-818-4; Print ISBN:

978-1-61779-817-7



Reference Books: 1. Plant Biotechnology. 2000. Hammond J, McGarvey P.& Yusibov V. (Eds): Springer Verlag,. 2. Practical Application of plant molecular biology. 1997. Henry. R.J, Chapman and Hall.

Online Resources: NPTEL.nic.in Published on Apr 16, 2013:Youtube; http://shomusbiology.weebly.com/

Semester – Odd; Category: Generic Elective (GE) Course - UG


BGE 030 Marine Biotechnology 3 0 0 3 45


To impart knowledge on concepts of marine biota, marine bioactive products and the use of GE tools to produce commercially important products

On successful completion of the course, the student will be able to understand marine ecosystem and its importance to importance to humans in Biotechnology perspective

Marine Biotechnology

UNIT 1 Introduction: Marine Ecosystem, Marine Environment zonation: Pelagic, Benthic, Sub-littoral and Deep-Sea Environments; General classification and taxonomy of marine organisms: Bacteria, fungi, viruses, microalgae, invertebrates and vertebrates.

UNIT 2 Biological community structure and associations: Symbiosis, commensalism and antagonisms among different groups of organisms

UNIT 3 Marine microbiology Microenvironments: Biofilm formation, Biofouling Process, Quorum Sensing (QS); Survival in Adverse Conditions- Barophilic, thermophilic and halophilic, Bioremediation (PAHs, aliphatic hydrocarbons, heavy metals); Marine microbial chemical classes and therapeutic effects

UNIT 4 Marine Bioprospecting Marine organisms: Defense mechanisms (physical, chemical cues and/ or epiphytic load), Types of bioactive compounds with reference to antimicrobial, anticancer, pharmacological- analgesic, histaminic and other properties Isolation and identification of select marine bioactive compounds (alkaloids, flavonoids and polyketides) and depsipeptides. Marine Pharmaceutical companies (PharmaMar, Novartis, Hoffman La Roche, etc) and an overview of their products and their statuses in clinical trials and market

UNIT 5 GE Tools and methodologies in marine science Genetic Engineering of marine organisms: Micro and macroorganisms as research subjects- Transgenic fish: Growth hormone and anti-freeze proteins- methods, stages of transformation, vectors used, design of vectors, Production and identification of proteins and depsipeptides from invertebrates: Sponges, Molluscs and tunicates

UNIT 6 Commercial production of marine products Algal biotechnology- Properties, production and uses of: single cell protein, hydrocolloids (agarose, carrageenan, alginates), pigments (carotenoids and xanthophylls) and other by products

Text Books: 1. Marine Biotechnology: Pharmaceutical and bioactive natural products Vol I: by David H. Attaway,

Oskar R. Zaborsky, Plenum Press, New York (ISBN: 0-306-44174-8), 1993 2. Marine Biology- An ecological approach by James Nybakken- 6

th Edn, 2005, Pearson Publishers,

Benjamin Cummings, ISBN: 10-0805345825, ISBN: 13-9780805345827. Reference Books:

1. Biotechnology: Environmental Processes I, Volume 11a, Second Edition Editor(s): H.-J. Rehm, G. Reed, 2008, Wiley-VCH-GmbH, ISBN: 9783527283217

2. Fundamentals of Ecology by Eugene. P. Odum and Gay. W. Barrett, Publl: W. B. Saunders 5th

edition 2005

Online Resources:



1. http://www.marinebiotech.eu 2. http://www.lsi.umich.edu

Antimicrobial Agents

UNIT 1 Classification, structure and mode of action of antibacterial, antifungal, antiviral antibiotics

UNIT 2 Resistance to antimicrobial drugs, genetics of drug resistance and its spread. Biochemical mechanisms of drug resistance.

UNIT 3 Molecular principles of drug targeting against antibiotic resistant bacteria

UNIT 4 Peptide antibiotics, Phytochemicals as antimicrobial agents.

UNIT 5 Combination therapy - additive, synergistic and antagonistic antibiotic

UNIT 6 Practicals: Growth Inhibition Assays: Antibiotic Sensitivity Assay, Gradient Plate Technique, Minimum Inhibitory Concentration of Antibiotic, Bioautography

Text Books: 1. Essentials of Medical Pharmacology, 7

th Edition, 2013. KD Tripathi.

2. Phytochemical methods- a guide to modern techniques of plant analysis, 3rd ed. 1998. J B Harborne. Chapman & Hall

Reference Books: 1. Antimicrobial Agents, 2012 Varaprasad Bobbarala 2. Quality control in Herbal drugs- An approach to evaluation of botanicals. 2002. P K Mukherjee, Business Horizons Online Resources: http://www.microbiolab-bg.com/CLSI.pdf http://www.gxccl.com/download/upload/CLSIM100.pdf

Semester – ODD; Category: Generic Elective (GE) Course- UG

Course Number

Course Code


BGE 032 Algal Biotechnology 3 0 0 3 45


To impart knowledge on concepts of taxonomy, production and phytoremediation using algae

The student will have a thorough understanding on the algal taxonomy, commercially important products and phytoremediation using algae.

Algal Biotechnology UNIT 1 Taxonomic classification of micro and macrolagae- Taxonomic classification of micro

and macroalgae: Habit, habitat and distribution, morphological features (appearance, pigments and life cycle, ecology: Cyanophyta (Spirulina, Nostoc and Anabaena),

Semester – ODD; Category: Generic Elective (GE) Course- PG

Course Number


BGE 031 Antimicrobial Agents 1 0 4 3 75 Objectives Learning outcomes

To impart knowledge on 1. Classification of antibiotics 2. Mechanism of action 3. Mechanism of resistance 4. Combined Antibiotic therapy 5. Plant Products as Antimicrobial Agents


Find the mechanism of action of the antibiotics

Determination of Minimum Inhibitory Concentration of the Antibiotic

Selecting the right choice of antibiotics for the resistant micro-organisms

Use appropriate antibiotics for combination therapy in the case of drug resistance

http://www.marinebiotech.eu/

http://www.microbiolab-bg.com/CLSI.pdf



Xanthophyta, Chlorophyta (Chlorococcus, Hematococcus and Ulva); Phaeophyta (Dictyota and Laminaria); Rhodophyta (Chondrus, Dunaliella and Gracilaria) and fossil algae. Numerical taxonomy of algae: dendrogram and phenogram, cluster analysis

UNIT 2 Phytoconstituents of algae: Proteins and amino acids, lipids, waxes, glycerol, vitamins, pigments (chlorophylls, carotenoids and phycobiliproteins) and polysaccharides: agar agar, algin and carageenans, Single cell Proteins (SCPs)

UNIT 3 Algaculture: Isolation of pure microalgal cultures- Types of culture media for microalgae – Isolation of pure cultures – Kinetics and Growth patterns, factors affecting growth (temperature, light, mixing, pH, salinity, oxygen and nutrients), Measurement of algal growth. Substrates and production system for SCP

UNIT 4 Production systems for macroalgae: Raceway pond culture and photobioreactors, harvesting- Centrifugation, flocculation and filtration. Extraction and processing of agar-agar and carageenans Biofuels: Methane and hydrogen production, energy and chemicals, Biofertilizers: Liquid seaweed fertilizer as phosphate solublizers and nitrogen fixers

UNIT 5 Phytoremediation: Algae used, remediation methods for treating heavy metals, dye decoloration and sewage water treatment

UNIT 6 Algae and pollution Harmful Algal Bloom (HAB)- red tide and associated hazards- shellfish poisoning, Eutrophication, Algae as indicator of pollution

Reference Books: 1. The Algae. Chapman V J (1962). Macmillan & Co. Ltd. 2. Properties and Products of Algae. Zajic, J. E. 1970. Plenum Press, New York. 3. Handbook of Hydrocolloids, Glyn O. Phillips, Peter A. Williams: Carrageenan, Imeson, A.P. FMC

Corporation (UK) Ltd, 5, 88-102 Online Resources: http://algae.ucsd.edu/research/ http://www.oilgae.com/ref/glos/algal_biotechnology.html

Nanotechnology Theory cum demonstration

UNIT 1 Introduction: History of Nanoscience; nanomaterials, nanosized effects surface to volume ratio, nanoscale molecular and atomic size, quantum effects.

UNIT 2 Nanomaterials: Synthesis of nanoparticles and nanofabrication – bottom up and top up approaches; chemical methods (sol-gel, sonochemical, solvothermal); physical methods (mechanical milling), colloidal routes and biological methods (microbes, green chemistry); Types of nanomaterials (Gold, Silver, Carbon, Ferro magnetic); Quantum dots; Graphene and Fullerenes.

UNIT - 3 Characterization of Nanoparticles: Structure and Size characterization of nanoparticles: XRD, TEM, SEM, AFM, Light scattering, UV-Vis spectroscopy, Surface Plasmon resonance, EDAX, ICP-MS; Properties of nanoparticles – rods, spheres, nanotubes (Gold, Silver, Carbon, Ferro magnetic)

UNIT - 4 Principles of Bionanotechnology: Energetics; Chemical transformation; Biomolecular Motors; Biomaterials; Traffic across membranes; Biomolecular sensing; self-replication

UNIT - 5 Nanosensors: Optical based detectors; Mechanical detectors; Lateral Flow; Electrochemical detectors; magentic sensors; applications of the various sensors in science.

UNIT - 6 Nanomedicine: Targeted delivery - drug, nucleic acid, Theranostics, Nanobodies, Antimicrobial activity of NPs. Toxicity of nanoparticles and their management, Bioethics and societal implications of nanotechnology

Semester – Odd; Category: Generic Elective - UG

Course Number


[BGE036] Nanotechnology 3 3 45


To impart knowledge on nanomaterials and their applications

On successful completion of the course, the student will be able to understand the applications of nanoparticles



Text Books 1. Nanostructures & Nanomaterials: Synthesis, Properties, and Applications by Guozhong Cao,

Imperial College Press, London, 2004. 2. Chemistry of Nanomaterials: Synthesis, properties and applications by CNR Rao et al., 3. Nanobiotechnology: Concepts, Applications and Perspectives. ed. C. M. Niemeyer, C.A. Mirkin.

2006. Wiley –VCH. Reference Books

1. Bionanotechnology: Lessons from Nature, David S Goodsell, 2004, Wiley publication 2. Biosensors and Biodetection: Volume 503: Optical based Detectors, Avraham Rasooly and Keith

E Herold, 2009, Humana Press 3. Biomolecule-Based Nanomaterials and Nanostructures Itamar Willner* and Bilha Willner DOI:

10.1021/nl102083j | Nano Lett. 2010, 10, 3805–3815 4. Gold nanoparticles in the clinical laboratory: principles of preparation and applications. Hassan

M.E. Azzazy, Mai M.H. Mansour, Tamer M. Samir and Ricardo Franco. Clin Chem Lab Med 2012;50(2):193–209. doi 10.1515/CCLM.2011.732

Online Resources: NPTEL.nic.in, http://nanohub.org/groups/biomed

Alternative models for Experimental Toxicology

Unit 1 Introduction to pharmacology & toxicology, history, classification of drugs and toxicants, Routes of exposure of toxicants.

Unit 2 Preclinical Toxicology: Acute toxicity, chronic toxicity, local tolerance study, immunotoxicity studies, genotoxicity, carcinogenicity studies, reproductive toxicity studies.

Unit 3 In vivo assay systems for drug targets and action: Alternative to animal models (fish, brine shrimp, and bacteria, onion root tip, potato disc assay using Agrobacterium) used for drug targets and action.

Unit 4 In vitro assay systems for drug targets and action: enzyme based, organ based, tissue based, and growth inhibition assays

Unit 5 Regulatory Toxicology: Drug Discovery and Development: Drug Laws, FDA, OECD, ICH guidelines. Schedule Y of the Drugs and Cosmetics Act for requirements of preclinical and clinical trial studies.

Unit 6 Practical demonstrations: Antibiotic Sensitivity Assay, Minimum Inhibitory Concentration, Bioautography Cytotoxicity Assay using Brine Shrimp Lethality Test, Genotoxicity Test using Onion Root Tip Assay, Determination of LD50 value of drug using Brine Shrimp and Zebra fish Model Organism.

Text Books: 1. Essentials of Medical Pharmacology, 6

th Edition, KD Tripathi.

2. Phytochemical methods- a guide to modern techniques of plant analysis, 3rd ed. 1998. J B Harborne. Chapman & Hall

Reference Books: 1. Pharmaceutical Biotechnology by Gary Walsh 2. Pharmacological Assays 3

rd Edition by Hans Vogel

3. Antimicrobial Agents, 2012 Varaprasad Bobbarala 4. Quality control in Herbal drugs- An approach to evaluation of botanicals. P K Mukherjee,

Business Horizons

Online Resources: http://www.microbiolab-bg.com/CLSI.pdf,

1. http://www.gxccl.com/download/upload/CLSIM100.pdf

Semester – Even; Category: Generic Elective- PG

Course Number


[BGE 037] Alternative models for Experimental Toxicology

2 1 3 45


To impart knowledge on 1. Various Toxicity Tests 2. In vitro and In vivo

bioassays

On successful completion of the course, the student will be able to:

Determine the LD50 value of the drug

Determination of Minimum Inhibitory Concentration of the Antibiotic

Bioactivity of the drug

http://www.microbiolab-bg.com/CLSI.pdf

http://www.gxccl.com/download/upload/CLSIM100.pdf



Scheme of Curriculum and Evaluation, 2015 Regulations

M Sc Biotechnology Degree Programme – Year 1; Semester 1, 2015

Co

urs

e N

um

ber

Course Code Cate

go

ry

Course Title

RG Credits/Week Hours/ semester (Credits x 15 weeks)

Att

end

ance

(%

)

Co

nti

nu

ou

s In

tern

al

assessm

en

t [T

heo

ry /

Pra

cti

cal]

(a)

(CIA

)

End Semester Assessment Grand Total

Lectu

re (

L)

Tuto

rial (T

)/ C

linic

al

train

ing (

CT

)

Pra

ctical

(P)/

Researc

h P

roje

ct

Cre

dit

s (

C)

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory

(b)

Pra

ctic

al/

Viv

a (c

)

Theory

: a+

(b÷2)

= 1

00 P

ractical:

a +

c =

100

EST ESP

1 PBT15CT101 CT1 Biochemistry of Biomolecules

4 - - 4 60 - 60 80 50 100 - 100

2 PBT15CT103 CT2 Enzymes and proteomics

4 - - 4 60 - 60 80 50 100 - 100

3 PBT15CT105 CT3 Analytical Techniques

4 - - 4 60 - 60 80 50 100 - 100

4 PBT15DE107 DE1 Choice - (any One) DE BDE001; BDE002; BDE003

3 - - 3 45 - 45 80 50 100 - 100

5 PBT15GE109 GE1 To be chosen

3 - - 3 45 - 45 80 50 100 - 100

6 PBT15AE111 AE1 Cell Biology

2 - - 2 30 - 30 80 50 100 - 100

7 PBT15AE113 AE 2 Basic Microbiology 2 - - 2 30 - 30 80 50 100 - 100

8 PBT15CL151 CL1 Biomolecules & Analytical techniques (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

9 PBT15CL153 CL2 Cell Biology and Microbiology (Practicals)

- - 3 3 - 90 90 80 50 - 50 100

Semester 1 Total 22 0 7 29 330 210 540 450 700 100 900



M Sc Biotechnology – Year 1; Semester-II, 2015

Co

urs

e N

um

ber

Course Code

Cate

go

ry

Course Title RG

Credits/Week Hours/ semester

(Credits x 15 weeks)

Att

end

ance (

%)

Co

ntin

uous I

nte

rnal

assessm

ent

[Th

eory

/ P

ractical] (

a)

(CIA

) End Semester Assessment

Grand Total

Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical tr

ain

ing

(CT

)

Pra

ctical

(P)/

Researc

h

Pro

ject

Cre

dit

s ©

Le

ct.

/ T

ut.

Pra

ctical/ C

R

To

tal

Ho

urs

Theory (b) Practical/ Viva (c)

Theory:

a+(b÷2) = 100

Practical: a + c = 100 EST ESP

10 PBT15CT102 CT4 Molecular Biology 4 - - 4 60 - 60 80 50 100 - 100

11 PBT15CT104 CT5 Bioprocess Technology

4 - - 4 60 - 60 80 50 100 - 100

12 PBT15CT106 CT6 Biological Calculations and Biostatistics

4 - - 4 60 - 60 80 50 100 - 100

13 PBT15DE108 DE2 Bioinformatics [BGE023]

2 - 1 3 30 30 60 80 50 100 - 100

14 PBT15GE110 GE2 Choice – (any One) 3 - - 3 45 - 45 80 50 100 - 100

15 PBT15CL152 CL3 Protein Methods (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

16 PBT15SL154 SL1 Animal Cell Culture Technology (Practicals)

1 - 1 2 15 30 45 80 50 - 50 100

Semester II Total 18 0 6 24 270 180 450

350 500 100 700



M Sc Biotechnology – Year 2; Semester-III, 2015

Co

urs

e N

um

ber

Course Code Cate

go

ry

Course Title

Credits/Week

Hours/ semester (Credits x 15 weeks)

Att

endance (

%)

Continuous I

nte

rnal

assessm

ent [T

heory

/

Pra

ctical] (

a)

(CIA

)


Grand Total

RG Lectu

re (

L)

Tuto

rial (T

)/

Clin

ical tr

ain

ing

(CT

) P

ractical

(P)/

Researc

h

Pro

ject

Cre

dit

s ©

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs

Theory (b) Practical/ Viva (c)

Theory: a+(b÷2) =

100 Practical: a + c = 100 EST ESP

17 PBT15CT201 CT7 Immunotechnology 4 - - 4 60 - 60 80 50 100 - 100

18 PBT15CT203 CT8 Cancer Biology 3 - - 3 45 - 45 80 50 100 - 100

19 PBT15CT205 CT9 Environmental Biotechnology

2 - 1 3 30 30 60 80 50 100 - 100

20 PBT15CT207 CT10 Genetic Engineering

3 - - 3 45 - 45 80 50 100 - 100

21 PBT15DE209 DE3 Pharmaceutical Biotechnology [BDE 004]

3 - - 3 45 - 45 80 50 100 - 100

22 PBT15AE211 AE3 Scientific Writing and Research methodology

- 2 - 2 - 60 60 80 50 100 - 100

23 PBT15CL251 CL4 Genetic Engineering (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

24 PBT15CL253 CL5 Cancer Biology & Immunotechnology (Practicals)

- - 4 4 - 120 120 80 50 - 50 100

Semester III Total 15 2 9 26 225 330 555

400 600 100 800



M Sc Biotechnology – Year 2; Semester-IV, 2015

Co

urs

e N

um

ber

Course Code

Cate

go

ry

Course Title

Credits/Week

Hours/ semester (Credits x 15 weeks)

Att

endance (

%)

Continuous

Inte

rnal

assessm

ent

[Theory

/ P

ractical]

(a)

(CIA

)


Grand Total

RG L

ectu

re (

L)

Tuto

rial (T

)/

Clin

ical

train

ing (

CT

) P

ractical

(P)/

Researc

h

Pro

ject

(RP

)

Cre

dit

s ©

Lect.

/ T

ut.

Pra

ctical/ C

R

To

tal H

ou

rs Theory (b) Practical/

Viva (c) Theory:

a+(b÷2) = 100

Practical: a + c = 100 EST ESP

25 PBT15DE202 DE4 (any one) 3 - - 3 45 0 45 80 50 100 - 100

26 PBT15RP252 RP Dissertation & Viva voce

- - 18 18 0 540 540 80 50 - 50

# 100

Semester IV Total 3 0 18 21 45 540 585

100 100 50 200

Grand total 58 2 40 100 870 1260 2130 1300 1900 350 2600

a: CIA Theory & Practical (Eligibility to appear for university examinations is 40% & 80% attendance)

b: EST (Pass Min. CIA & ESE Aggregate: Grade B [50%])

c: ESP (Pass Min. CIA & ESE Aggregate: Grade B [50%])

# ESP- the total of 50 % is determined from the evaluation weighted at 25% for the dissertation and 25% for the viva voce (W: 25 % each)



Comparison of Credits for 2015 batch and 2016 batch of admissions

Year of admission

Sem1 Sem2 Sem3 Sem4 total

2015 29 24 26 21 100

2016 26 27 26 21 100

The syllabi for the common courses in semester I and II onwards of CBCS students admitted in 2015 has been included in these regulations and is applicable from this batch of students. The syllabi for the unique courses already conducted for 2015, are enclosed herein. Highlighted courses are common and syllabi available supra.

Syllabus for M Sc Biotechnology admission batch 2015

First Year – Semester – I; Category Core

Course Number


2 PBT15CT103 Enzymes and proteomics 4 4 60 Objectives Learning outcomes

To impart knowledge on Structure; functions and analysis of proteins in cells; enzymes and its analysis

On successful completion of the course, the student will be able to ● comprehend the structure of the proteins ● functions of proteins and enzymes ● analysis of proteins by various methods ● bioinformatics tools for protein analysis

2. ENZYMES AND PROTEOMICS UNIT 1 Protein structure, Outline of Protein synthesis & degradation; chemistry of amino acids and

polypeptides; overview of the levels of protein structure; forces involved in determining protein structure and Conformation

UNIT 2 Denaturation and renaturation of proteins, protein folding, protein structure and prediction (swissprot), co- and post- translation modification of protein; structure and function of signal peptides – signal hypothesis – protein targeting and translocation

UNIT 3 Introduction to Enzymes General properties of enzymes, functional organization of enzyme- domains, (multi enzyme complex); active site of enzyme

UNIT 4 Nomenclature and catalysis of enzymes Nomenclature and classification and importance of enzymes; Coenzymes, Metal ions in enzyme catalysis and Isoenzymes

UNIT 5 Basic Concepts in Enzyme Kinetics Free energy, activation energy, enzyme substrate complex, Kinetics- . Michaelis – Menten kinetics; kinetic parameters- Km, Vmax, Kcat; Lineweaver Burke plot, Factors affecting enzyme activity; Enzyme inhibition – types of inhibition.

UNIT 6 Purification of enzymes: Enzyme sources- plant, animal and microbial. Extraction of Membrane bound proteins, intracellular and extracellular enzymes, precipitation, Ion exchange, Gel permeation and Affinity chromatographic separation; Enzyme assay- measurement of enzyme activity- specific activity, enzyme units- IU, katal

UNIT 7 Proteomics and In silico analysis: Electrophoretic Separation of proteins-SDS-PAGE, Native and , 2D electrophoresis Protein sequencing, MALDI-TOF, LC-MSMS, application of proteomics, Bioinformatics tools for proteomics – GST protein analysis , MASCOT, Molecular docking- receptor- ligand interactions using insilico tools , Biological active peptides Mini project involving some proteomic tools - retrieval of sequences (FASTA), homology search, clustalW analysis, phylogenetic analysis for protein (Mega6)

Text Books 1. Principles and Techniques of Biochemistry and Molecular Biology – Keith Wilson and John Walker.

7th Edition, Cambridge University Press, 2010.

2. Biochemistry - Donald Voet and Judith G.Voet, 4th Edition, 2011, John Wiley & sons Inc.

3. Principles of Biochemistry. A.Lehninger et al,6th Edition, UBS publishers.

4. Biochemistry. L.Stryer. W.H.1999, Freeman and company. 5. Biochemistry. Zubay, 1998, Freeman and company.



Reference Books 1. Biochemical Calculations, Irwin H. Segel, John Wiley and Sons Inc. 2. General Chemistry, Linus Pauling, W.H. Freeman and company. 3. Organic Chemistry, DJ Cram and GS Hammond, McGraw Hill.

Online Resources http://www.separations.us.tosohbioscience.com/ServiceSupport/TechSupport/ResourceCenter/PrinciplesofChromatography/IonExchange http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html https://www.chem.purdue.edu/courses/chm333/web%20Site%202.htm http://www.wiley.com/legacy/college/boyer/0470003790/links/links_list.htm#visual http://nptel.ac.in/

First Year – Semester - I Category: Ability Enhancement Courses (AE)

Course Number


6 PBT15AE111 Cell Biology 2 - - 2 30


To impart knowledge on the various aspects of cells like organelles, membrane, transport.

On successful completion of the course, the student will be able to - ● comprehend the cell organelle, cell membrane ● signal transduction and its implications ● cell cycle and its relevance in cancer

6 Cell Biology

UNIT 1 Study of cells: Cell theory; Diversity of cell size, shape, structure and organization in bacteria, yeast, plant and animal cells; Endosymbiotic theory.

UNIT 2 Structure, organisation and functions of cells: Cell organization - prokaryotic and Eukaryotic cells. Cell walls and Membranes; Structure and function of cell organelles: Nucleus, Nucleolus, Endoplasmic reticulum, Golgi apparatus, Vacuoles, Lysosome, Ribosomes, Cytoskeletal systems; Cell adhesion

UNIT 3 Thermodynamics and Transport: Laws of Thermodynamics – first and second; Enthalpy, Entropy, Gibb‟s free energy; biological equilbria, Nernst equation; membrane proteins and transport of nutrients (active, passive, osmosis); protein trafficking; transport across nucleus; transport across membranes.

UNIT 4 Hormone and Signal transduction: Principles of cell signalling – GPCR, Enzyme-linked cell surface receptors, Ion channel linked receptors, Tyrosine Kinase receptor, Jak/STAT, Calcium, Non-Kinase (wnt, hedgehog); Hormones (lipophilic, peptide, growth factors).

UNIT 5 Cell Cycle and Apoptosis: Cell cycle (Mitosis and Meiosis); Components of cell cycle – cyclins, CDKs; Cell cycle check points; Cell cycle regulation; Programmed cell death (PCD); types of PCD (Necrosis, Apoptosis, Autophagy, Anoikis)


th Edition.




7. Gene VII Benjamin Lewin, Oxford University Press, U.K., 2000. 8. Biochemistry & Molecular Biology. William H Elliott and D.C.Elliott, Oxford University Press,

2000. 9. Reproduction in Eukaryotic cells. DM Prescott, Academic Press. 10. Principles of Genetics by Gardner, Simmions&Snustad 1991.

http://bcs.whfreeman.com/thelifewire/content/chp06/0602001.html

https://www.chem.purdue.edu/courses/chm333/web%20Site%202.htm

http://www.wiley.com/legacy/college/boyer/0470003790/links/links_list.htm#visual



Online resources: 1. NCBJ Book Store 2. http://nptel.ac.in/

7 Basic Microbiology

UNIT 1 Introduction to Microbiology: History and scope of microbiology, Nature of microorganisms, Basic features of bacteria-,Size, shape, arrangement of membrane and cell wall), cytoplasmic inclusions, mesosomes, flagella and motility, slime, capsule, pili, chemotaxis and endospore. Bacterial chromosome, nucleoid, plasmid (types and function). Reproduction in bacteria, Morphology of bacteria, Normal bacterial flora of the body.

UNIT 2 Microbial taxonomy – Principles of taxonomy, Classical characteristics used in taxonomy, Microbial evolution and diversity. Bergey‟s Manual of Systematic Bacteriology.

UNIT 3 Concept of Sterilization & Isolation of microbes Sterilization principles, Physical and Chemical methods of sterilization; disinfection, sanitization, antisepsis, sterilants and fumigation , Isolation of pure cultures

UNIT 4 Microscopy Simple and compound Microscopy – Dark field – Phase contrast – Fluorescence and Electron Microscopy

UNIT 5 Stains and Staining techniques - 4 hours Preparing smears, simple stains, Differential stains and special staining (Endospore, capsular & Flagella).

Text Books: 1. Dubey RC and Maheswari DK (2005). A Text book of Microbiology. S.Chand &Company Ltd., New

Delhi. 2. Powar CB and Daginawala HF (2005). General Microbiology, Volume I & II, 8th Edition, Himalaya

Publishing House, Mumbai. 3. Sundara Rajan S (2003). College Microbiology. Volume 1 & 2. Revised Edition, Vardhana

Publications, Bangalore.

Reference Books:

1. Pelczar Tr. MJ, Chan ECS & Kreig NR (1993). Microbiology MC Graw-Hill Inc., New York. 2. Robert F Boyd (1984). General Microbiology. Times Mirror/Mosby College Publishers. 3. Prescott LM, JP Harley and DA Klein (1993). Microbiology, 2nd Edition, WM, C Brown Publishers.

Online Resources:

1. http://media.johnwiley.com.au/product_data/excerpt/54/14443346/1444334654-85.pdf 2. http://course.wilkes.edu/microbio/

First Year – Semester - I Category: Ability Enhancement Courses (AE)

Course Number


7 PBT15AE113 Basic Microbiology 2 - - 2 30


To have basic knowledge about microorganisms and their ubiquitous presence in nature To gain knowledge in asepsis and microbiological techniques

On successful completion of the course, the student will be able to identify and isolating microbes Students also will gain knowledge in bacterial taxonomy and microbial techniques.

http://nptel.ac.in/



Practical Manuals:

2. Laboratory Manual in Microbiology, Gunasekaran. P (2009), New Age international Pvt Ltd

Publishers, ISBN-10: 8122407838.

2. Cell Biology Protocols, J. Robin Harris, John M. Graham, David Rickwood,(2006), Wiley, ISBN-

9780470847589

3 Microbiology: A Laboratory Manual, James G. Cappuccino, Natalie Sherman,(2013), Pearson

Education, Limited, ISBN-9780321864864.

Online resources:

4. http://web.calstatela.edu/faculty/rnissen/biol380/BIOL380LabManualSP10.pdf

5. http://homepages.uconn.edu/~mb2225vc/MCB_2225/Course_overview__h1_MCB2225

6. http://study.com/academy/topic/microbiology-laboratory-techniques.html

First Year – Semester - I Core Lab

Course Number

Course Code


9 PG15 BTCL153

Biology of cells and microbes 3 3 90


To impart knowledge on techniques used to study cells and microbes

On successful completion of the course, the student will be able to - Understand the basics of techniques to study cells Basics of microscopy Aseptic techniques and microbial culture methods

No. 9. Lab in Biology of cells and microbes

1 Microscopy: Bright field and phase contrast with representative cell types- bacteria, fungal, algal, plant and animal cells

2 Isolation of cell types using gradient centrifugation and their staining

3 Histochemical techniques: Onion Root Tip Mitosis

4 Histochemical analysis of Meiosis – Meiosis slides

5 Osmotic analysis or Plasmolysis – potato or carrot or onion cells

6 Micrometry for cell enumeration and cell size determination

7 Cell motility assay.

8 FACS – demonstration

9 Microbiology:

10 Sterilization techniques – autoclaving, heat sterilization, filtration, UV irradiation and Chemicals

11 Media and Slant preparation: Liquid media, Solid media

12 Isolation and cultivation of pure cultures of bacteria

13 Microscopy and staining of bacteria: Simple staining, differential staining, Capsule staining, Acid –fast staining and Spore staining

14 Kirby-Bauer method of antibiotic susceptibility test

15 Biochemical test for identification of bacteria: Catalase test, Oxidase test, IMViC test, Starch degradation test, Carbohydrate fermentation

16 Growth curve by using bacterial culture



List of Examiners: M Sc Biotechnology 2015 onwards

Sl. No.

Name of examiner Designation & Address Contact information

1. S.Vijayasri-

Asst. Professor, SRM University, Katankulathur

Old 10, New 19, Second Floor, Krishnamoorthy Street, West Mambalam Chennai- 600033

2. Mr Asrar, Asst. Professor, New College, phone: 9445371337

3. Dr. P. Kalaiselvi, M.Sc., Ph.D.,

Lecturer Medical Biochemistry Department of Medical Biochemistry Dr.ALM PGIBMS University of Madras, Taramani, Chennai-600 113. Door no. 2, III Floor, I C Block, Vasant Apts, 100 Feet Bye Pass Road, Vijaya Nagar, Velachery, Chennai-42 Land mark Opp. Sai Sarovar

Tel:24540767 E- mail address : [email protected] Cell: 9841371726

4. Dr Rajeswari,D Lecturer, Dept. of Biochemistry, Dr MGR Univ. of Science and Technology,

09444564099

5. Dr K Sreekumar Pondicherry Univ.

Reader, Dept of Biochemistry, Pondicherry University

09443616641

6. Ms Shankari D, HoD, Dept. of Biotechnology, Faculty of Sciences and Humantities, SRM University, Kattangulathur – 603 203

09884145827

7 Dr. T. B. Sridharan Associate Professor, CBST, VIT UNIVERSITY, Vellore - 632 014, Tamil Nadu, India

Resi:7/4 Kumaran Street, Vettuvanam post, Via Pallikonda. PIN 635 809 Vellore District Tamilnadu India. email: [email protected]@gmail.com Ph: 948 667 7314

8 Dr Raveen, MCC Professor, Dept. of Zoology, MCC 09840463064

9 Dr R Saravanan Dr Saravanan R, Assistant Professor, Cheyyar Govt. Arts and Science College Dept. of Zoology Postal Adress: Dr R Saravanan, G41, Fourth Floor (Madura Gardens), 491 (new No. 15), Poonamallee High Road, Chennai – 600 095

R: 42611260 Mobile: 9952077967 Email: [email protected]

10 Dr Preethi Mehta, Lecturer, Dept. of Biotechnology, WCC College, Chennai

Contact: 9840256752; 2641 1078

11 Dr Devi, Phone:

Assistant Professor, SRM University, Zoologist; At MIOt for STEM cell;

09444907563; [Email: [email protected]]

12 Dr. N. SUDHAKAR, M. Sc, M. Phil, Ph.D.,.

Associate Professor ; Dept. of Biotechnology, Dr.M.G.R. Educational and Research Institute University, Maduravoyal, Chennai-600 095

Email : [email protected], [email protected]; Cell: 91-9566031265

13 Dr R Malathi, Professor, Department of Genetics, Dr. AL Mudaliar PG IBMS,University of Madras

09941874714

14 Dr K Veena Gayathri;

Asst. Prof. Dept. of Biotechnology; Stella Maris College, Chennai

No. 37, G2, Ground Floor; EGB Amsam Apartments, Govindan Road; West Mamabalam, Chennai – 6000 33 Email: [email protected]; 09940412463;044-24748750

15 Dr C Kamatchi Asst. Prof. Biotechnology Dept..; MGR University

9094463807

16 Dr Rajasekar T Biochemistry; Asst. Prof. SS Dept. of Biotech. REC; Thandalam; Chennai- 602 105

Ph: 9524095108

mailto:[email protected]

http://us.mc354.mail.yahoo.com/mc/[email protected]







Sl. No.


17 Dr. P.Agastian Assistant professor,

Dept of Plant Biology & Biotechnology, Loyola Collaege, Chennai – 600 034

Email: [email protected] Mobile: 9444433117

18 Dr. J. Arunakaran, Ph.D.,

Senior Lecturer; Department of Endocrinology Dr. ALM Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai – 600 113

Telephone:22552698 Phone No: 24480794 Ext No: 28/29 E-mail address : [email protected]; [email protected]

19 Dr S Vijayalakshmi,

Dept. of Biotechnology, Vels University Mob: 09445168222 Land Line: 044- 24988031 Email: [email protected]

20 Rama Vaidyanathan, Ph.D Professor and Head – Biotechnology Dr. M.G.R. Educational and Research Institute (Dr. M.G.R. University) E.V.R. Periyar Salai, Maduravoyal Chennai – 600 095, Tamil Nadu, INDIA

www.drmgrdu.ac.in Mob: 98410 02846

21 Dr D Carol

Assistant Professor, Biomedical Instrumentation Science, Dept. of Advanced Zoology & Biotechnology, Loyola college;

[email protected] Mobile: 09840958069 Dr Caroline "Carol Pereira" [email protected];

22 Prof. J Richardson,

Dept of Zoology, Govt. ARTS and Science, Mens‟ College, Nandanam

Contact No: 9841919183

23 Dr Nandhini Devi G Associate Professor, Anna University Centre for Biotechnology, A.C.Tech., Anna University Sardar Patel Road, Guindy,Chennai-25

(O) 2235 8378 Email [email protected], [email protected]

24 Dr Rex Arunraj

Asst. Professor Dept. of Genetic Engineering School of engineering SRM Univ. Kattankulathur Chennai- 603 203

044-27400189 [email protected] [email protected] 09444025651

25 Dr Ramya Mohandas,

Assistant Professor SRM- school of Bioengineering, SRM "Ramya Mohandass" <[email protected]>

Mobile: 09442044277 SRM University, Kattangulathur

26 Janarthanan Sundaram Associate Professor Department of Zoology University of Madras Chennai-600 025, Tamil Nadu, India

Cell:09486362887,off. (Tele.):91-44-22202831 Fax: 91-44-22301003

27 Dr. M. Saravanan, SRM Assistant Professor (Senior Grade)

Assistant Professor (SG), Department of Biotechnology (FISH), SRM University SRM Nagar, Kattankulathur, Chennai - 603203.Tamil Nadu, India.

Mail: [email protected], [email protected] Phone Off : +91-44-27452270 Mobile +91- 9443077097

28 Dr. B. Usha,

Assistant Professor, Area: Genetic Engineering and Genomics; Department of Genetic Engineering, Kattankulathur Campus, SRM University

Phone No: 09176057901

29 Dr. R.RAVINDHARAN Chennai – 600 034 Mobile: 9840486271

Assistant professor, Dept of Plant Biology & Biotechnology, Loyola College,

Email: [email protected]

30 Dr. S. Kirankumar;

Assistant Professor (SG); Department of Genetic Engineering, SRM University Area: Cancer Biology, Zebrafish Genomics

Mail: [email protected]


http://www.unom.ac.in/endofac3.html

mailto:jarun.endo@unom

http://www.drmgrdu.ac.in/










Sl. No.


31 Dr.S.Sureshkannan. M.V.Sc.,Ph.D,

Assistant Professor (SS) Bioinformatics and ARIS Cell, Madras Veterinary College, TANUVAS, Chennai -600051.

32 Dr Venkat Kumar, Senior Lecturer, School of Biotechnology VIT

919894698150

33 Dr Sathish kumar CBST, VIT

VIT UNIVERSITY, Vellore - 632 014, Tamil Nadu, India

34 Dr. C. Sudandira Doss, PhD.,

Assistant Professor (Senior) School of Biosciences and Technology VIT University, Vellore. 632 014. Tamilnadu State, India.

Mobile: +91 9443072826 Phone: +91 416 2257194 Fax: +91 416 2243092 E.mail: [email protected]

35 Leslie Lawrence Department of Botany Madras Christian College Tambaram, Chennai - 59

[email protected] Phone: 09380715155

36 Dr. Mathangi Adhoc Lecturer, CBT, Anna University 9962102981 0r 24986040

Old No. 16/ New 8, Flat 7, Lakshmi Apartment, Justice Sundram Rd., Mylapore, Chennai- 4

37 Dr. T. B. Sridharan

Associate Professor Plant Molecular Biology, VIT UNIVERSITY, Vellore - 632 014, Tamil Nadu, Indi

Resi: 7/4 Kumaran Street, Vettuvanam Post, Via Pallikonda. PIN 635 809Vellore District Tamilnadu India. email: [email protected] [email protected] Ph: 948 667 7314

38 Mr Asrar Asst. Professor, Dept. of Zoology, New College, Chennai

phone: 9445371337

39 Dr Raveen Professor, Dept. of Zoology, MCC 09840463064

40 Dr K PurnaSai Scientist, Biomaterials Division, CLRI, TICEL BIOPARK, Tharamani, Chennai- 600 113, india

09444502584 [email protected]; [email protected]

41 Dr Saleesha A Stanley,

Prof. and Head; Dept. of Biotechnology; Jeppiaar Engineering College Jeppiaar Nagar, Rajiv Gandhi Salai (OMR), Chennai- 600 119

Tel: 044-24502818 extn. 313; Email: [email protected]

42 Dr Vidyalakshmi, Asst. Prof. Ethiraj Colege Botany Dept. 09840126136

43 Dr G Ramesh Kumar

Scientist, AU-KBC Research Centre, MIT campus, Anna University, Chromepet, Chennai 600 044

94443133310 [email protected]

45 Dr Swaminathan Principal, Vivekananda College, Chennai 09444079954

46 Dr Revathi, HOD DEPT. of Zoology The Ethiraj College for Women,

[email protected] [email protected]

47 Rama Vaidyanathan, Ph.D Professor and Head – Biotechnology, Dr. M.G.R. Educational and Research Institute(Deemed University) E.V.R. Periyar Salai, Maduravoyal Chennai - 600 095, Tamil Nadu, INDIA

www.drmgrdu.ac.in Mob: 98410 02846

http://m.v.sc/








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