18th Annual RESEARCH DAY

FRIDAY, FEBRUARY 22, 2008

USF Health Rotunda

07:30a.m. - 08:30a.m. Session “A” and Session “C” Registration and Poster Set Up

08:30a.m. - 10:00a.m. Session “A” and Session “C” Poster Presentation Viewing

and Judging 10:00a.m. - 10:15a.m. Session “A” ONLY Poster Take Down 10:00a.m. - 10:15a.m. Session “B” Registration 10:15a.m. - 10:30a.m. Session “B” Poster Set Up 10:30a.m. - 11:45a.m. Session “B” and Session “C” Poster Presentation Viewing

and Judging 11:45a.m. - 12:00p.m. All Posters Taken Down USF Health North Breezeway 11:45a.m. - 12:45p.m. Lunch for Presenters and Judges (ticket required) USF Health Auditorium 12:45p.m. - 01:00p.m. Special Recognition of Distinguished USF Health Faculty 01:00p.m. - 02:00p.m. 12th Annual Roy H. Behnke Distinguished Lectureship 02:00p.m. - 02:15p.m. Refreshments in Breezeway area 02:15p.m. - 04:15p.m. USF Health Invited Graduate Student Oral Presentations

USF Health Rotunda 04:30p.m. - 06:00p.m. Awards Ceremony and Reception

18th Annual RESEARCH DAY

FRIDAY, FEBRUARY 22, 2008

Poster Presentation Section Topics

Session “C” 8:30a.m. - 11:45a.m.

• Signature Interdisciplinary Program in Allergy, Immunology,

and Infectious Disease

Session “A” 8:30a.m. - 10:00a.m.

• Biomedical Engineering and Drug Discovery • Cancer Biology • Cardiovascular Sciences • Computational Biology and Technology • Environmental and Global Health • Family and General Health • Neuroscience • Occupational and Workplace Issues • Other Clinical Studies and Case Reports

Session “B”

10:30a.m. - 11:45a.m.

• Biomedical Engineering and Drug Discovery • Cancer Biology • Cardiovascular Sciences • Computational Biology and Technology • Environmental and Global Health • Family and General Health • Neuroscience

Other Clinical Studies and Case Reports

Dear Colleagues: If you pause and look back at the last few years of our journey together, you will be astounded by what we have been able to accomplish. Excellence in research and graduate education has already resulted in the University of South Florida being designated as Carnegie Comprehensive Doctoral Research University/Very High Research Activity; one of only 95 public and private universities in the U.S. which have received this elite distinction. Earlier this year, under the leadership of President Judy Genshaft, the University articulated a bold and comprehensive strategic plan to continue our ascendancy towards greatness and seek an invitation to become a member of the Association of American Universities. We, at USF Health are convinced that as in the past, we will play a defining role in the accomplishment of this vision. As such, under the tutelage of Dr. Steve Klasko, Vice President USF Health and Dean, College of Medicine, and in partnership with Drs. Patricia Burns (Dean, College of Nursing) and Donna Petersen (Dean, College of Public Health), we have initiated the implementation of a USF Health-wide plan aimed towards continued enhancement of our research capacity and further improvement in our research infrastructure. The recent recruitment/appointment of Dr. Wilbur Milhous from Walter Reed (Associate Dean for Research, COPH), Dr. Kevin Kip from the University of Pittsburgh (Executive Director of Research, CON), and Dr. Patricia Emmanuel (Associate Dean for Clinical Research, COM) are just some of the examples of how we are transforming our research support infrastructure at this Institution. In the current academic year alone, largely due to the beneficence of our donors, we have been able to establish much needed state-of-the-art core facilities in Advanced Microscopy & Cell Imaging, Flow Cytometry and Cell Sorting, Cardiovascular and Small Animal Imaging, Neurobehavior and Telemetry, Biostatistics, etc. Our goal for the next academic year is to establish a world-class outpatient clinical research center on our North campus and to continue to enhance our existing capacity in the areas of proteomics and genomics. Establishment of an Advanced Neuroimaging Center for small and large animals is also on the horizon.

Interdisciplinary translational and clinical research is an extremely important component of our portfolio. Working in partnership with our affiliates, we have established a Clinical and Translational Science Institute which will provide the required infrastructure to create a unique and productive nexus between basic and clinical scientists. It is the home for the NIH-funded physician-scientist training program (K30) and the CTSA Planning grant. It is branching out in the areas of evidence-based medicine and health informatics and will soon become a formidable resource for investigators in the University system.

Our graduate and post-doctoral programs continue to thrive. The establishment of many new and innovative Master-level programs and the realignment of our doctoral programs to meet the emerging global needs have been very rewarding. Given the University’s global outreach, distance-learning has been a high priority at USF Health. In Fall 2008, the Office of Graduate & Postdoctoral Studies in the College of Medicine will begin offering an online Masters program in Medical Sciences and many more are in the pipeline.

Our faculty recruitment efforts have been extremely rewarding. Dr. Robert Pedowitz, a world-class academic surgeon was recruited from the University of California San Diego to lead the Department of Orthopedic Surgery, COM. Similarly, Dr. Clifton Gooch, a renowned neuromuscular specialist, has been recruited from Columbia to lead the Department of Neurology, COM. The Infectious Disease focus in the Department of Global Health, COPH has resulted in the recruitment of two Florida World Class Scholars (Dr. Thomas Unnasch from UAB and Dr. John Adams from Norte Dame) who join a highly visible and NIH-funded team at this College.

Despite declining NIH funding, our existing faculty have been extremely successful in obtaining new competitive grants from this agency. How competitive are our investigators is perhaps best exemplified by the recent NIH award to Dr. Jeffrey Krischer of $169 million to study the triggers of juvenile diabetes. This international study will be conducted in four countries and will follow more than 7,800 newborns genetically at risk for type I diabetes. Due to your continued hard work and unprecedented commitment to outstanding interdisciplinary basic, translational, and clinical research, year-to-date extramural funding at USF Health (excluding our affiliate partners) has increased by ~85%; from $53 million (in FY 06-07) to $98 million in FY 07-08. Despite the highly competitive funding environment, this upward trend is primarily due to doubling in funding from Federal sources.

We are at a “tipping” point in our relatively young history. Together, we are transforming the culture of this Institution and leading the change in academic medicine in the Nation. Our commitment to excellence is unwavering, our vision is bold, our strategy is comprehensive, and our outlook is global…this is the definition of your USF Health.

Sincerely,

Abdul S. Rao, MD, MA, DPhil Professor of Surgery & Molecular Medicine Senior Associate Vice President – USF Health

18th Annual RESEARCH DAY

Sponsored By:

College of Medicine

College of Nursing

College of Public Health

School of Biomedical Sciences

School of Physical Therapy and Rehabilitation Sciences

Signature Interdisciplinary Program in Allergy, Immunology, and Infectious Disease

Department of Internal Medicine, College of Medicine

Thermo Fisher Scientific, Inc.

Sweetbay Supermarket

18th Annual RESEARCH DAY

12th Annual Roy H. Behnke Distinguished Lectureship

PLACE: USF Health Auditorium

TIME: 12:45p.m. - 2:00p.m.

SPEAKER: James R. Baker, Jr. M.D. Ruth Dow Doan Professor

Professor of Internal Medicine Director, Michigan Nanotechnology Institute for Medicine & Biological Sciences

University of Michigan

TITLE: “MEDICAL APPLICATIONS OF NANOTECHNOLOGY”

Sponsored by: Signature Interdisciplinary Program in Allergy, Immunology,

and Infectious Disease College of Medicine, USF

James R. Baker, Jr. M.D. Ruth Dow Doan Professor Professor of Internal Medicine Director, Michigan Nanotechnology Institute for Medicine & Biological Sciences University of Michigan Biographical Sketch

Dr. Baker joined the faculty of the University of Michigan in 1989 and is currently Professor of Medicine and Division Chief of Allergy and Clinical Immunology in the Department of Internal Medicine, Professor of Pathology, and Professor of Biomedical Engineering in the School of Engineering at the University of Michigan. In July, 1998 Dr. Baker was appointed Director of the U-M's newly organized Center for Biologic Nanotechnology and in 2001 was inaugurated as the first recipient of the Ruth Dow Doan Endowed Professorship in Biologic Nanotechnology. Following the success of the Center for Biologic Nanotechnology, in April 2005, U-M's Board of Regents formed the Michigan Nanotechnology Institute for Medicine and Biological Sciences (M-NIMBS) and appointed Dr. Baker as its first Director. Under his leadership, M-NIMBS merges academic expertise and institutional resources across the university to develop and market applications for nanotechnology in medicine, the biological sciences and the environment.

Dr. Baker's research includes the application of nanomaterials to cellular engineering, drug

delivery and gene transfer, and is supported by over $35 million dollars in federal grants and contracts. These studies have produced new vector systems for gene transfer using dendritic polymers with potential to revolutionize pharmaceutical therapy. Dr. Baker’s work with synthetic lipid and polymeric nanostructures has resulted in the development of a new class of antimicrobial agents with activity against bacteria, spores, fungi and viruses. These projects led to two start-up biotechnology companies, NanoBio Corporation and Avidimer Therapeutics, both located in Ann Arbor, Michigan where Dr. Baker serves as the Chief Scientific Officer of both corporations.

Dr. Baker is recognized as both a national and international leader in the fields of Allergy and Clinical Immunology and Biologic Nanotechnology and has attained significant recognition and awards for his work including the 2001 U-M Dean's Innovation Award, given to faculty members whose innovations radically improved or transformed clinic outcomes, educational processes, or research processes. Dr. Baker serves on the editorial boards of three noteworthy journals and is one of three editors of the National Nanotechnology Initiatives’ Research Directives. Dr. Baker has chaired numerous study sections for NIH and serves on many internal and external advisory boards and committees including the Nanotechnology Technical Advisory Group (N-TAG) of the President's Council of Advisors on Science and Technology (PCAST) for the Executive Office of the President of the United States, the advisory to a sub-committee of the Defense Intelligence Agency and in 2006 began a 5 year appointment as a Director of the American Board of Allergy and Immunology (ABAI).

ADMINISTRATION

Stephen K. Klasko, MD, MBA Senior Vice President, USF Health Dean, College of Medicine

Abdul Rao, MD, MA, D Phil Senior Associate Vice President - USF Health Vice Dean for Research & Graduate Studies, College of Medicine Director, School of Biomedical Sciences, College of Medicine Patricia A. Burns, PhD, RN, FAAN Dean, College of Nursing Donna J. Petersen, MHS, ScD Dean, College of Public Health Wilbur K. Milhous, PhD Associate Dean for Research, College of Public Health Jessica Banko, PhD Director of Research Services, College of Medicine Kevin Kip, PhD Executive Director of the Research Center, College of Nursing

SIGNATURE INTERDISCIPLINARY PROGRAM IN

ALLERGY, IMMUNOLOGY, AND INFECTIOUS DISEASE COLLEGE OF MEDICINE

Shyam Mohapatra, PhD, Director Basic Sciences, Professor of Internal Medicine, Pediatrics,

Environmental and Occupational Health and Molecular Medicine

John Sinnott, MD FACP, Director Clinical Sciences, Professor of Internal Medicine, Surgery, Obstetrics/Gynecology, Molecular Medicine and Mental Health Law and Policy/C&G

COLLEGE OF MEDICINE

Policy Committee Committee on Research

2007-2008

COLLEGE OF MEDICINE FACULTY

Kersti Linask, PhD Co-Chair, Committee on Research Kenneth Ugen, PhD Co-Chair, Committee on Research

BASIC SCIENTISTS Jay Dean, PhD Molecular Pharmacology and Physiology Carmelina Gemma, PhD Neurosurgery Kersti Linask, PhD Pediatrics Min You, PhD Molecular Pharmacology and Physiology Kenneth Ugen, PhD Molecular Medicine

DEAN’S APPOINTMENTS (AT LARGE) George Blanck, PhD Molecular Medicine Esteban Celis, MD, PhD DIO Svetlana Garbuzova-Davis, PhD Neurosurgery Gabor Legradi, PhD Pathology and Cell Biology Diane Straub, MD Pediatrics Mary Zhang, PhD Pathology and Cell Biology Xue-Zhong Yu, MD DIO

CLINICAL SCIENTISTS Sarah Cowgill, MD Surgery Morna Dorsey, MD Pediatrics Maya Guglin, MD, PhD Internal Medicine Kathleen Rockefeller, PT, MPH Physical Therapy Kenneth Zuckerman, MD DIO

OTHER COMMITTEE MEMBERS Chris Leonardo Graduate Student (ex officio) Jessica Banko, PhD Administrative Officer

18th Annual RESEARCH DAY

STAFF ACKNOWLEDGEMENTS

USF Health Office of Research

Cheryl Lesko Charmaine Disimile

Harold Paul Christine Tam Denise Flores

USF Health Media Center

Eric Younghans Daisy Jelks Caryl Loper

College of Medicine Office of Research

Jo Ann Moore Rochelle Morris Matthew Rutter

College of Medicine Signature Interdisciplinary Programs

Nicole Crawford Stephanie Medley

College of Medicine IS

Moayad Alzoubi Eduardo Mendez

College of Medicine Public Affairs

Klaus Herdocia

USF Health Development Georgia Windham

College of Public Health

Ellen Kent

USF Undergraduate Research Office Penny Carlton

Naomi Yavneh, PhD

18th Annual RESEARCH DAY

POSTERBOARDS

Location: USF Health Rotunda, College of Nursing Foyer and Atrium SESSIONS A and C Set up time: 7:30am - 8:30am SESSION A Take down: 10:00am SESSION B Set up time: 10:00am - 10:30am SESSIONS B and C Take down: Noon Poster Size: 4 ft by 6 ft.

JUDGING OF POSTERS

Student posters will be judged by faculty members.

You must be at your poster to be eligible for judging.

Posters will be judged on: 1. Presentation of poster

• Organization • Readability • Appearance • Sense Appeal

2. Presentation of data

• Oral Communication

3. Knowledge of subject • Question and answer presentation

AWARD CEREMONY

Winners will be announced at the Awards Ceremony following the USF Health Invited Graduate Student Oral Presentations

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2 0 0 8RESEARCH DAY

ABSTRACTS

Disclaimer: Abstracts printed within are for USF Health internal use only.

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RESEARCH DAY 2008 ABSTRACT TABLE OF CONTENTS

Anderson, Nicole O-1 Corbin, Karen O-2 de la Cruz, Cara O-3 Kong, William O-4 Vesley, Brian O-5

Agudelo, Marisela C-1 Ajmo, Joanne C-2 Bedoya, Felipe C-3 Chootang, Patchanee C-4 Dajani, Nagwa C-5 Glaum, Mark C-6 Holloway, Mary Jolene C-7 Hussain, Syed C-8 Kandasamy, Ruckmani C-9 Karolat, Joerg C-10 Kong, Xiaoyuan C-11 Liu, Guoqing C-12 Lukas, Susan C-13 Mendonca, Sarita C-14 Monpetit Jones, Alison C-15 Ntumngia, Francis C-16 Park, Seungjoo C-17 Patel, Tanmaykumar C-18 Peng, Yanhua C-19 Rogers, Chris C-20 Saenz, Fabian C-21 Sherwood, Tracy C-22 Shirley,Shawna C-23 Signarovitz, Aimee C-24 Smith, Shelly C-25 Tucker, Matthew C-26 Wang, Jia-Wang C-27 Wang, Xihai C-28 Woodrow, Jane C-29 Xu, Weidong C-30 Zhang, Weidong C-31

ABSTRACT # ORAL PRESENTATIONS

SESSION C – SPAIID ABSTRACTS

SESSION A: BIOMEDICAL ENGINEERING AND DRUG

DISCOVERYButtrice, Audrey A-32Cross, Richard A-33Fox, Dawn A-34Kumar, Arun Babu A-35Lendrihas, Thomas A-36Mahajan, Shikha A-37Schumacher, Joshua A-38Williams, Eva A-39

SESSION A: CANCER BIOLOGYBewry, Nadine A-40Caire, M. Thure A-41Dobson, Toni A-42Drenberg, Christina A-43Khambaty, Maria A-44Lengacher, Cecile A-45Ma, Qiuping A-46Parikh, Vevek A-47Sallman, David A-48Shaheed, Aisha A-49

SESSION A: CARDIOVASCULAR SCIENCESBoone, Brian A-50Gal, Shaili A-51Guyer, Adam A-52Norring, Sarah A-53Schwetz, Tara A-54

SESSION A: COMPUTATIONAL BIOLOGY AND TECHNOLOGY

Agravat, Manoj A-55Barbier, George A-56Ibrahimou, Boubakari A-57Malo, Teri A-58Tu, Yicheng A-59Wahi, Monika A-60

SESSION A: ENVIRONMENTAL & GLOBAL HEALTHArevalo, Mariana A-61Siegel, Kristy A-62Zeager, Michelle A-63

SESSION “A” ABSTRACTS

SESSION A: FAMILY & GENERAL HEALTHAlipour, Kent A-64 Armstrong, Kathleen A-65 Daley, Ellen A-66 Greenough, Candace A-67 Jevitt, Cecilia A-68 Kukla, Rebecca A-69 Liller, Karen A-70 Mendoza, Alejandro A-71 Wilson, Crystal A-72

SESSION A: NEUROSCIENCEBadanich, Kimberly A-73 Hou, Huayan A-74 Jiminez, Jeffy A-75 Koren, John A-76 Lee, Daniel A-77 Lunquist, Quinn A-78 Rrapo, Elona A-79 Schlunk, Andrea A-80 Sponaugle, Ashley A-81 To, Chung A-82 Virkud, Winay A-83 Womble, Tracy A-84 Yesudas, Jeremy A-85 Zhu, Yuyan A-86

SESSION A: OCCUPATIONAL & WORKPLACE ISSUES

Acree Conlon, Helen A-87 Debroux, Sophia A-88 Gallant-Roman, Mary A-89 Hadsell, Mary A-90 Jagusztyn, Nicole A-91 Logan, Jennifer A-92 Marty, Adam A-93 Mohammed, Sheila A-94 Shockley, Kristen A-95 Yang, Liu-Qin A-96

SESSION A: OTHER CLINICAL STUDIES & CASE REPORTS

Al-Saadi, Sam A-97 Boger, Jan A-98 Bowen, Byers A-99 Bozorg, Ali A-100 Carey, Stephanie A-101 Guthrie, Jason A-102 Herweg, Bengt A-103 Highsmith, M. Jason A-104

SESSION A: OTHER CLINICAL STUDIES & CASE REPORTS (cont’d)

Jara, Sandra A-105 Logan, Jennifer A-106 Mateja, Candice A-107 Nagamia, Sameer A-108 Palumbo, Brian A-109 Phillips, Joshua A-110 Postler, Gilbert A-111 Purcell, Ronald A-112 Rakita, Steven A-113 Rasheid, Sowsan A-114 Rideout, Drew A-115 Sagatys, Elizabeth A-116 Schrock, Christopher A-117 Sterrett, Ashley A-118 Thometz, Donald A-119 Torella, Tracy A-120 Valdes, Michelle A-121 Varghese, Mathew A-122 Vasher, Marie A-123 Wilson, Steve A-124 Witmer, Matthew A-125 Zoble, Robert A-126

SESSION B: BIOMEDICAL ENGINEERING & DRUG DISCOVERY Bucher, Cindy B-32 Guerges, Mina B-33 Hu, Xiangdong B-34 Kumar, Arun B-35 Murphy, Robin B-36 Mutka, Tina B-37

SESSION B: CANCER BIOLOGYAnsari, Mujiba B-38 Lambert, Vance B-39 Patel, Delaura B-40 Shen, Zheng B-41 Wang, Xiaoqin B-42 Watson, Jordan B-43 Zhang, Yingtao B-44

SESSION B: CARDIOVASCULAR SCIENCESEve, David B-45 Lastra Vicente, Rosana B-46 Serrano, Maria B-47

SESSION “B” ABSTRACTS

SESSION B: COMPUTATIONAL BIOLOGY & TECHNOLOGY RESEARCH

Ahmed, Aysha B-48Ahmed, Saqib B-49Bostick, Jaclyn B-50Karim, Saya B-51Raj, Opal B-52Wadhwa, Sanju B-53

SESSION B: ENVIRONMENTAL GLOBAL HEALTHGray, Monica B-54Johnson, Giffe B-55Riley, Tunisia B-56Michael, Ryan B-57

SESSION B: FAMILY & GENERAL HEALTHAugust, Euna B-58Blunt, Heather B-59Dyer, Karen B-60Eftekhari, Roxanna B-61Estefan, Lianne B-62Fuhrmann, Hollie B-63Helmy, Hannah B-64Kolar, Stephanie B-65Salinas, Abraham B-66Shaffer, Emily B-67Smith, Sarah B-68Thomas, Kamilah B-69Vamos, Cheryl B-70Williams, Jillian B-71

SESSION B: NEUROSCIENCEAbisambra, Jose B-72Cao, Chuanhai B-73Carty, Niki B-74Chaparro, Rafael Eduardo B-75Erasso, Diana B-76Etheredge, LaTia B-77Jernberg, Jennifer B-78Lebson, Lori B-79Ott, Mackenzie B-80Shapshak, Paul B-81

SESSION B: OTHER CLINICAL STUDIES AND CASE REPORTS Arledge, John B-82Aya-ay, Melanie B-83Badri, Vinil B-84Bagg, Andrew B-85Baluch, Aliyah B-86Branch, John B-87Brown, Natalie B-88Cantor, Edisson B-89Draper, Nicole B-90

Dreier, Jonathan B-91

SESSION B: OTHER CLINICAL STUDIES & CASE REPORTS (cont’d)

Edwards, Paul B-92 Estores, David B-93 Hunter, Joel B-94 Joshi, Sunir B-95 Kay, Christine B-96 Konin, Jeff B-97 Kucera, Stephen B-98 Leigh, Jennifer B-99 Lin, Kaiwen B-100 Marulanda, German B-101 Morton, Conner B-102 Moses, Helen B-103 Padhya, Tapan B-104 Pierre, Marie B-105 Prakash, Sateesh B-106 Richards, David B-107 Roland, Samantha B-108 Ruiz, Rene B-109 Schocken, Dawn B-110 Sharma, Neil B-111 Shin, Byol B-112 Toomey, Paul B-113 Valeriano, Joanne B-114

USF HEALTH

INVITED GRADUATE STUDENT

ORAL PRESENTATIONS

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Abstract #: O-1 Presented by: Nicole Anderson, BS, Graduate Student Bcl-2 as a Novel Marker for Ovarian Cancer

Nicole Anderson,,Yira Bermudez 1, Donna Badgwell 2, Ren Chen 3, Robert C. Bast 2, Patricia A. Kruk 1 1 USF COM, Department of Pathology and Cell Biology, 2 University of Texas, M. D. Anderson Cancer Center, 3 USF COM, Office of Clinical Research, Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Bcl-2, ovarian cancer, CA125, biomarkers

Objective: CA125 is the only FDA-approved blood test for the detection of epithelial ovarian cancer, but both its sensitivity and specificity are low. B-cell lymphoma 2 (Bcl-2) protein has been shown to prevent apoptosis through inhibition of cytochrome c release from the mitochondria. Since Bcl-2 has been shown to be elevated in many solid tumors, including ovarian cancer tumors, we examined whether Bcl-2 could be a novel biomarker for this deadly gynecologic disease. Methods: Urine from normal healthy volunteers (n=128) and patients with either benign gynecologic disease (n=130) or ovarian cancer (n=141) was obtained from H. Lee Moffitt Cancer Center and M. D. Anderson Cancer Center. Bcl-2 ELISA was performed on these samples to determine the levels of Bcl-2 present in urine of normal patients versus cancer patients. Samples were tested in triplicate and results expressed as the average ng/mL Bcl-2 ± standard error. Results: We found that Bcl-2 levels in the urine of ovarian cancer patients are significantly higher than that of normal patients or patients with benign gynecological disease. In combination with CA125 values, elevated urinary levels of Bcl-2 improved detection of ovarian cancer to more than 98%. Likewise, low urinary values of Bcl-2 reduced CA125 false positives associated with benign gynecologic disease to less than 2%. Conclusion: These data suggest that urinary Bcl-2 may be a useful novel biomarker for ovarian cancer and, in combination with CA125, may have improved clinical efficacy for detection of ovarian cancer. Research supported by: This research was funded, in part, by DOD OCRP #W81XWH-07-0276.

Abstract #: O-2 Presented by: Karen Corbin, MS, Graduate Student Unraveling the Complex Mechanisms that Control Nitric Oxide Production and Vascular Health Karen Corbin, Laura C. Pendleton- USF COM, Department of Molecular Medicine, John M. Koomen- H. Lee Moffitt Cancer Center and Research Institute Proteomics Core, Larry P. Solomonson- USF COM Department of Molecular Medicine, Duane C. Eichler- USF COM Department of Molecular Medicine, Basic Biomedical Sciences Keywords: Nitric Oxide Vascular Health Proteomics Objective: Impairment of nitric oxide (NO) production and metabolism has been associated with a number of vascular disorders. Studies have clearly delineated the role of endothelial nitric oxide synthase (eNOS) in the regulated production of NO. Much less is known about argininosuccinate synthase (AS), an enzyme that provides the substrate for eNOS catalyzed NO production and is essential for endothelial cell viability. We have designed our studies to understand the various control points that regulate the role of AS in endothelial NO production. Methods: We have utilized a combination of biochemical, in silico and proteomic approaches to assess the regulation of AS expression, post-translational modifications and protein interactions. Results: Our data indicates that several stimuli regulate the expression of AS in a manner consistent their function in the production of NO. In addition, we have identified the first known phosphorylation sites in endothelial AS and have begun to uncover their roles in the regulation of NO production. Finally, we have identified several key AS protein interactions that support its central role in vascular health. Conclusion: Our studies have expanded the traditional views of the control of NO production from an eNOS-centric focus to a more global perspective via the combination of basic molecular techniques and proteomic approaches. Given the indispensable role of AS in vascular health, this enzyme may be an attractive therapeutic target for cardiovascular disorders. Research supported by: American Heart Association, Florida Affiliate, Grant & Predoctoral Fellowship; University of South Florida Research Foundation- Mary and Walter Traskiewicz Memorial Fund; Signature Interdisciplinary Program in Cardiovascular Research Pilot Grant

Abstract #: O-3 Presented by: Cara de la Cruz, MPH, Graduate Student Examining Maternal Length of Hospital Stay at Delivery As A Risk Factor for Depressive Symptoms Postpartum Cara de la Cruz, MPH,Jason Salemi, M.P.H., Department of Epidemiology and Biostatistics, COPH USF,Caroline Peterson, D.C., Department of Anthropology, CAS & Department of Epidemiology and Biostatistics, Department of Community & Family Health,COPH USF Keywords: postpartum depression, maternal complications, length of stay Objective: To examine the association between mother’s length of hospital stay (LOS), which serves as a proxy for severity of maternal complications, and symptoms of PPD among women who had recently given birth. Methods: Data were obtained from North Carolina’s 2004 Pregnancy Risk Assessment Monitoring System. This population-based study sample included 1,248 women aged 14 to 55. Maternal LOS was categorized into short (vaginal delivery 0-1 days, cesarean section (c-section) 0-3 days); normal (vaginal 2-3 days, c-section 4-5 days); prolonged (vaginal 4-6 days, c-section 6-8 days); and very prolonged (vaginal 7+ days, c-section 9+ days). Self-reported symptoms of PPD were determined from the following question: “Since your new baby was born, how often have you felt down, depressed or hopeless?” “Always” and “often/almost always” were categorized as depressed, while “sometimes,” “rarely,” or “never” were not depressed. Logistic regression was performed to estimate the odds ratio (OR) and 95% confidence interval between LOS and PPD symptoms. Results:

11.9 percent reported symptoms of PPD. Multivariable logistic regression analysis revealed that, compared to women with a short LOS, women with a normal LOS were more likely to report PPD symptoms (OR=1.11;1.05-1.17) and women with slightly prolonged LOS were less likely to report PPD symptoms (OR=.95; 0.88-1.03). Women with a very prolonged LOS were 96% less likely to report PPD symptoms (OR=0.51; 0.44-0.61). Conclusion:

Further research may clarify the effects of LOS and subsequent symptoms of PPD, particularly why women experiencing very prolonged LOS are least likely, and women with normal LOS most likely, to report symptoms of PPD.

Abstract #: O-4 Presented by: William Kong, BS, Graduate Student miRNA Profile of TGF-b Induced EMT: miR-155 Contributes to Epithelial Cell Plasticity through RhoA William Kong, Hua Yang, Lili He, Jian-jun Zhao, Domenico Coppola, Jin Q. Cheng, Molecular Oncology Program and Department of Pathology and Cell Biology, USF COM, H. Lee Moffitt Cancer Center and Research Institute, Keywords: mirna, tgf-b, emt, metastasis, rhoa Objective: TGF-beta facilitates metastasis in breast cancer through a number of genes. However, miRNAs regulated by TGF-beta remain elusive. Since TGF-beta induced epithelial-to- mesenchymal transition (EMT) in NMuMG is a common cellular model for studying the mechanisms of metastasis, we used this system to perform a miRNA expression profile. Methods: Hybridized a custom miRNA array with RNA isolated from parental and Smad4 knockdown (does not undergo EMT in presence of TGF-beta) NMuMG cells treated with vehicle or TGF-beta for 24 and 36 hours. Verified miRNA expression by Northern. Knockdown and overexpressed miRNAs using antisense 2'-O-methyl oligoribonucleotides (AsO) and vector based system, respectively, to perform in vitro cancer metastasis and invasion assays. Determined potential miRNA targets using bioinformatics, and confirmed by western blot and luciferase reporter assays. Performed in situ miRNA staining and qRT-PCR on invasive/ metastatic breast cancer tissue. Results: miR-155 found to be most significantly up-regulated. Knockdown and over-expression of miR-155 with immunofluorescent microscopy imaging, wound healing assays, cell migration and invasion studies found miR-155 to play a role in TGF-beta induced EMT. Identified and verfied RhoA as a target by western and reporter assay system. Invasive/metastatic breast cancer tissues show a high correlation with miR-155 expression. Conclusion: In summary, we report a miRNA signature of TGF-beta induced EMT in NMuMG cells. miR-155 mediated TGF-beta induced EMT, cell migration and invasion through RhoA. Further, expression of miR-155 is associated with invasive phenotype of breast cancer. Thus, miR-155 could be a potential target for breast cancer metastasis intervention. Research supported by: NIH, DOD

Abstract #: O-5 Presented by: Brian Vesley, BS, Graduate Student Quantification of parasitemia in Leishmania donovani-Infected Hamsters by real-time PCR Brian Vesley, Azliyati Azizan, Mark Sweat, Dennis Kyle, USF COPH, Department of Global Health Keywords: Visceral leishmaniasis, real-time PCR Objective: Visceral leishmaniasis affects over a half million individuals in 88 countries each year and is caused by the intracellular parasite Leishmania donovani. New drugs are urgently required to treat this devastating disease. Preclinical evaluation of new drugs is modeled in hamsters infected with L. donovani, although measurement of parasite loads is difficult and time consuming. In the current study a real-time PCR assay has been optimized and validated against two other methods to estimate the parasite load within the liver of infected hamsters. Methods: Reverse and forward primers were used that amplify a 120-bp target template of the kinetoplast DNA. Serial ten-fold dilutions of parasites were either assayed directly or spiked into un-infected liver homogenates. Quantification of the parasite load in infected-hamster livers was analyzed by a SYBR Green-based PCR assay and compared to microscopic and growth dilution assays. Results: Standard curves were generated from parasite cultures and parasite-spiked tissue. L. donovani DNA was readily detected in spiked homogenates and no products were observed with un-infected tissue and non-template control samples. The real time PCR assay was able to detect as few as 2 parasites in 2 mg of liver. In addition quantitative differences in parasite load were detected at low levels where microscopic confirmation was not possible. Conclusion: The real time PCR method is sensitive and specific for the quantification of the parasite load within L. donovani-infected hamsters, which will accelerate the evaluation of new drugs in the definitive preclinical model of disease. Research supported by: Bill and Melinda Gates Foundation

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Session “C”

SPAIID

ABSTRACTS

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Abstract #: C-1 Presented by: Marisela Agudelo, BS, Graduate Student Cannabinoid Receptor 2 (CB2) Mediates IgE Switching In Cultures of Murine Purified B Lymphocytes. Marisela Agudelo, Cathy Newton, Ray Widen, Tracy Sherwood, Liang Nong, Herman Friedman, and Thomas W. Klein. USF COM, Department of Molecular Medicine, School of Biomedical Sciences Keywords: B lymphocytes; immunoglobulin class switching; cannabinoids; IL-4; CB2. Objective: Marijuana cannabinoid treatment increases Th2 activity and previous reports showed B cells express the highest level of CB2 mRNA relative to other immune cells suggesting that cannabinoids play a critical role in B cell activation and maturation. We previously reported evidence of Th2 biasing and class switching in cannabinoid treated and antigen challenged mice. We now explore the possibility that cannabinoids directly influence B cell antibody class switching. Methods: C57BL/6 mouse splenic B cells were purified by negative selection and cultured with IL4 and anti-CD40 in the presence or absence of the nonselective cannabinoid agonist, CP55940, or the CB1 selective cannabinoid agonist, methanandamide, and analyzed at different days by flow cytometry and ELISA for surface expression and secretion of IgE. CB2 receptor expression was measured by flow cytometry. A CB2-mediated mechanism was also tested by measuring forskolin-induced cAMP accumulation after cannabinoid treatment. CB2 is coupled predominantly through Gi/o proteins, i.e., negatively through the G? subunit to adenylyl cyclase and positively through G?/? to MAP kinase. Results: Cells treated with CP55940 showed an increase in expression of IgE by day 5 in culture; methanandamide had no effect. CP55940 also induced an increase in secreted IgE in culture supernatants as analyzed by ELISA. In addition, CB2 receptors were increased on B cells following stimulation with IL-4 and anti-CD40 and the class switching effect of CP55940 was attenuated by the CB2 antagonist, SR144528. Conclusion: Our results suggest that cannabinoids bias toward Th2-type immunity by directly inducing B cell class switching from IgM to IgE through a mechanism involving CB2 receptors. Research supported by: Supported by NIDA grant #DA019824.

Abstract #: C-2 Presented by: Joanne Ajmo, PhD, Postdoc Adiponectin Normalizes LPS- and Acetaldehyde-Stimulated TNF-Alpha Production in Live Kupffer Cells Through Regulation of AMPK and SIRT1 Signaling. Joanne Ajmo,X. Liang; C.Q. Rogers; M.M. Murr; M. You. USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Adiponectin, hepatitis, macrophage, lippopolysaccharide (LPS), Tumor necrosis factor alpha (TNFalpha) Objective: Adiponectin plays an important role in the regulation of hepatic inflammation in vivo. Alcoholic liver injury is associated with reduced circulating adiponectin levels and increased secretion of hepatic pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-A). Methods: We investigated the protective role of adiponectin against ethanol-induced pro-inflammatory activity using two well characterized cell lines, RAW264.7 macrophage and rat Kupffer cells (RKC1), and explored the potential molecular mechanisms through which the protective effects of adiponectin may work. Results: Treatment of RAW264.7 macrophage or RKC1 cells with lipopolysaccharide (LPS) or acetaldehyde (ACE), a major product of ethanol metabolism, markedly increased TNF-A production. However, with the concurrent addition of globular adiponectin (gAcrp30) with LPS or ACE, there was a significant decrease (p< 0.05) in TNF-A as measured by ELISA compared to cells treated with LPS or ACE alone. Further mechanistic studies revealed that the activity of AMP-activated kinase (AMPK), a well known mediator of adiponectin signaling, was significantly decreased with the addition of LPS or ACE, but recovered to control levels when co-incubated with gAcrp30. Interestingly, while LPS or ACE significantly reduced protein levels of mammalian sirtuin 1 (SIRT1), a NAD+-dependent class III histone deacetylase, gAcrp30 treatment restored the SIRT1 protein levels. Conclusion: Taken together, our findings suggest that adiponectin influences LPS- or ACE-stimulated TNF-A production in liver Kupffer cells and these actions may be mediated through the activation of AMPK or SIRT1 signaling. Research supported by: This study was supported by NIH/NIAAA grants AA013623 and AA015951 (to M.You).

Abstract #: C-3 Presented by: Felipe Bedoya, MS, Graduate Student Pyrin-Only Protein 2 (POP2): A New Human Gene Involved In the Modulation Of The Immune Response Felipe Bedoya,Laurel Sandler(1), Jonathan Harton(2) 1. Department of Molecular Medicine, College of Medicine, USF 2. Center for Immunology and Microbial Disease, Albany Medical College, Albany, NY. USF College of Medicine Basic Biomedical Sciences - Molecular Medicine Keywords: Pyrin Immunology Inflammation Objective: To characterize and determine the cellular function of a new human gene identified in our lab, denominated POP2. Methods: Bioinformatics, Cloning,RT-PCR,Cell culture,DNA Transfection,Luciferase assay,Western Blotting, Immunofluorescence Microscopy Results: POP2 is a 294 nt single exon gene located on human chromosome 3 encoding a 97-aa protein with sequence and predicted structural similarity to other pyrin domains (PYDs). POP2 is expressed principally in peripheral blood leukocytes and displays both cytoplasmic and nuclear expression patterns. TNF-alpha-stimulated and p65 (RelA)-induced NF-kB-dependent gene transcription is inhibited by POP2 by a mechanism involving changes in NF-kB nuclear distribution. POP2 colocalizes with ASC in perinuclear apoptotic structures reducing their formation. Conclusion: POP2 is a negative regulator of NF-kB activity that influences the assembly of PYD-dependent complexes involved in apoptosis and inflammation. Proteins, such as POP2, that can disrupt or otherwise impede PYD-protein processes might have broad impact on cellular development, survival, or homeostasis by contributing to the control of apoptotic programs. Further, POP2 may represent a mechanism for restraining proinflammatory activation events by slowing the inflammasome formation, for terminating inflammatory signals and processes by limiting the duration of inflammasomes, or both. A better understanding of the mechanistic properties of such molecules capable of modulating NF-kB may ultimately provide alternative therapeutic strategies for the broad spectrum of diseases associated with sustained NF-kB activity including chronic inflammation, autoimmunity diseases, and cancer. (The Journal of Immunology, 2007, 178: 3837–3845)

Abstract #: C-4 Presented by: Patchanee Chootong, MS, Staff B- Cell epitopes in Duffy Binding Protein Patchanee Chootong, Kelly Van Buskirk, Francis B. Ntumngia, Moses Biasor, Christopher L. King, John H. Adams USF COPH, Department ofGlobal Health Keywords: B-Cell Epitopes, Duffy Binding Protein Objective: Plasmodium vivax infects 70-80 million persons per year worldwide, causing substantial morbidity. The Plasmodium vivax Duffy binding protein (DBP) plays a critical role in maintaining the erythrocytic cycle of P. vivax infection, making it an important candidate for inclusion in a vaccine against this parasite. The interaction of DBP with its receptor, the Duffy blood group antigen, is required for junction formation, the first irreversible step of invasion. Naturally occurring antibodies to DBP are prevalent in individuals living in areas of high malaria endemicity, but individuals show distinct quantitative and qualitative differences in their serological responses to the antigen. Polymorphisms in the dbp gene are clustered in its ligand domain, suggesting immune selection pressure. Methods: We studied the anti-DBP-specific responses in the residents of a malaria endemic region of Papua New Guinea (PNG), which is highly endemic for P. vivax Results: Measurement of antigen-specific titers to DBP demonstrated significant changes in titer over time. Surprisingly, a range of inhibition from complete to none was observed even among high responders to the DBP, indicating that some anti-DBPII antibodies are considerably more effective at blocking binding. Using these immune sera we identified 12 B-cell eitopes that correlated with the protective inhibitory responses. All but one of the B cell epitopes are surface exposed on the DBP ligand domain and consistent with the strain specific nature of the responses many of the epitopes contain variant residues. Conclusion: Our results provides the evidence for further study in vaccine development by targeting DBP adhesion molecules. Research supported by: NIH RO1AIO64478-01A1

Abstract #: C-5 Presented by: Nagwa Dajani, MD, Faculty Increased Endogenous Production of a Primitive CD34+ Hematopoietic Stem Population in Pregnant Women Nagwa Dajani, USF COM Department of Neurosurgery, Maura Manion, CON Charlie Szekeres COM, Maureen Groer, CON Keywords: Stem cells, Microchimerism, pregnancy, embryonic Objective: Earlier studies on fetal microchimerism showed that maternal blood contains CD34+ hematopoietic stem cells (HSCs) that bear paternal genetic markers or male phenotype, suggesting that these cells circulated to the mother from male fetuses during pregnancy. The objectives of this study are to investigate the effect of pregnancy on the frequency, characteristics and functions of maternal CD34+ HSCs, and 2. to determine the origin of these HSCs. Methods: Peripheral blood mononuclear cells (PBMC) were collected from pregnant, parous, nuliparous women and men and isolated by Ficoll density centrifugation. Samples were stained with anti -CD3, CD14, CD16, CD19, CD20, CD56 (FITC) lineage antibody cocktail. Samples were further stained with anti-CD 117 PerCp-Cy5.5, CD 34 (Pe-Cy 7), CD14 7 (APC-Cy 7) and SSEA-4 (APC), and -OCT ¾ (PE) antibodies. Data was collected with a FACSAria three-laser cytometer (and analyzed using FlowJo software. Results: A primitive population of CD34+ HSCs that expresses the embryonic markers Oct 4 and SSEA4 can be detected in the peripheral blood of pregnant and post partum women. Quantitative analysis showed significant increase in CD34+ HSCs in pregnant women compared to mulliparous women and men. A unique subpopulation of these cells was detected only in blood samples from pregnant women and expressed the embryonic markers Oct4 and SSEA4. Conclusion: Pregnancy stimulates the increased production of CD34+ HSCs. Expression of embryonic markers on these cells indicates that they may be of fetal origin, or alternatively represent a unique population of maternal derived cells. Ongoing genetic and functional analysis should determine the origin of these cells. This phenomenon could potentially favor postpartum regenerative capacity. Research supported by: Supported in part by 2RO1NR0050000

Abstract #: C-6 Presented by: Mark Glaum, MD, PhD, Faculty Identification of a Stable Human Mast Line Derived from Human Cord Blood Stem Cells Mark Glaum, MD, PhD, Guoqing Liu, Richard F. Lockey and Shyam S. Mohapatra, USF COM Department of Internal Medicine Keywords: Stem Cell, Mast Cell, Asthma Objective: Study of human mast cell activation is limited by availability of few mast cell lines and their need for expensive exogenous stem cell factor (SCF), IL-6 and IL-3. Therefore, the possibility that mast cell precursors present in human umbilical cord blood (HUCB) might be sustained in culture in the absence of exogenous cytokines was investigated. Methods: Stem cells were isolated from HUCB using CD133 bead selection, and purified cells were cultured in recombinant human SCF, IL-6 and IL-3 for 14 days. After continued culture in SCF and IL-6, single cell cultures were established in 96 well-plates using only culture medium and 2% fetal bovine serum (FBS). After 3 months, surviving colonies were examined for surface expression of CD117 and Fc?R1 and for intracellular expression of tryptase and chymase. Colonies were primed with biotinylated human myeloma IgE and challenged with control buffer, streptavidin (100ng/ml) and ionomycin (2.5 ?M). Cell supernatants were examined for beta-hexosaminidase (B-Hex) release at 30 min. Results: Of 10 surviving colonies, one expresses both Fc?R1 and CD117 on its surface as demonstrated by flow cytometry. Immunocytochemical staining shows expression of both tryptase and chymase. Transmission electron microscopy shows intracellular architecture typical of mast cell progenitors. IgE cross-linking and ionomycin-challenge induces 30 to 50% B-Hex release at 30 min, while buffer alone shows no effect. Conclusion: These findings suggest that SCF-independent mast cell precursors exist in HUCB, and the derived cell line, USF-CB1, may serve as a convenient experimental model to study human mast cell biology.

Abstract #: C-7 Presented by: Mary Jolene Holloway, BS, Graduate Student Characterization of Cytochrome b5 and Cb5 Reductase in the Fungal Pathogen, Candida albicans Mary Jolene Holloway, Andreas Seyfang, PhD; Michael Barber, DPhil; Department of Molecular Medicine, USF COM Basic Biomedical Sciences - Molecular Medicine Keywords: Candida, Cytochrome b5 Reductase, Cytochrome b5, Antifungal Drug Targets, Biochemical Characterization Objective: Candida albicans is a commensal member of the human microflora that is the most common causative agent of fungal-related disease. Emerging drug resistance is a major problem in Candida, contributed by enzymes involved in the detoxification of xenobiotics and pharmacological agents. One such enzyme, cytochrome b5 reductase (cb5r), has a high pharmacological significance owing to its role in fatty acid elongation, cholesterol biosynthesis, and cytochrome P450-mediated detoxification of xenobiotics. The objective of this project will be to study Candida cb5r and its electron acceptor, cytochrome b5 (cb5), as potential novel drug targets. Methods: We will perform biochemical, kinetic, and pharmacological characterization of Candida cb5r and cb5 following heterologous expression in E.coli. Furthermore, we will utilize protein pull-down assays to identify the protein-protein interactions of cb5r and cb5, with other cytoplasmic and/or membrane-bound proteins. Results: We have cloned the genes for both proteins and optimized their heterologous expression in E.coli. Parameters included temperature, IPTG concentration, and growth medium. Phylogenetic analysis revealed that both cb5r and cb5 from Candida have highest homology to their plant counterparts (67% and 75%), but lower homology to human cb5r and cb5 (55% and 68%). Conclusion: This characterization of cb5r and cb5 may help determine key factors involved in drug resistance in Candida. Interestingly, plant cb5r has been identified as a novel herbicidal target, making these plant inhibitors promising candidates as a new class of antifungals against Candida cb5r. Identification of novel drug targets has particular significance to combat multiple drug resistance in this opportunistic microbial pathogen.

Abstract #: C-8 Presented by: Syed Hussain, Undergraduate Cytokine Modulation in the Rat-Mouse Model of Sertoli Cell-Induced Donor Specific Immune Tolerance Syed Hussain, S. Park, Dept. Biology, USF; L. Patrick and L. Scaglione, Dept. Pathology & Cell Biology, USFCOM; N. Dajani, Center for Aging-Neuroscience, USFCOM; M. Groer, USFCON; D.F. Cameron, Department of Pathology & Cell Biology, USFCOM, USF CAS, Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: Xenogeneic Sertoli cells, immune system cytokines Objective: Extra-testicular Sertoli cells (SCET) have been shown to immunoprotect allo- and xenogeneic cell grafts in co-transplantation protocols and can induce donor specific immune tolerance. The mechanism by which SCET immunoprotect transplanted cells, however, is yet to be defined. The objective of this study was to determine the effects rat SCET on mouse cytokines in the rat-mouse model of Sertoli cell induced tolerance. Methods: Rat SCET and rat peritubular cells (PT-control) and vehicle (sham) were injected (IV) into the mouse host. One or three weeks later, rat skin was transplanted onto some mice. Mouse blood was collected weekly from controls, mice receiving only SCET, mice receiving only skin transplants and mice receiving first rat SCET and then rat skin. Results: Results indicate that INF-? was not different from control values 1 week following SCET injection and in SCET pre-transplanted mice 2 and 3 weeks post-skin transplantation. In the absence of SCET there was a significant elevation of this cytokine following skin transplantation. Pilot data indicates that 3 weeks following skin transplantation in the SCET pre-transplanted mouse, there is a significant reduction of IL-1a, IL-1b, IL-2, IL-6 and a reduction trend of IL-10 and IL-12 compared to controls. Also, a reduction of these cytokines is indicated in mice receiving only SCET transplants for 6 weeks. Conclusion: Results suggest that the systemic (IV) transplantation of rat SCET has a marked effect on immune system related cytokines and that this cytokine modulation is associated with the induction of donor specific immune tolerance in the mouse. Research supported by: USF Foundation

Abstract #: C-9 Presented by: Ruckmani Kandasamy, PhD, Postdoc Npra Inhibitor Isatin Inhibits Inflammation in an Experimental Model of Asthma Ruckmani Kandasamy,Sang Joon Park, Xiaoqin Wang, R F.Lockey and S.S. Mohapatra USF COM, Department of Internal Medicine Keywords: NPRA, ISatin, Asthma, Inflammation Objective: Atrial natriuretic peptide (ANP) plays a significant role in shaping the early immune response to environmental antigens and appears to be involved in polarizing the immune response to allergens. Thus, ability to alter the magnitude of natriuretic peptide receptor A (NPRA) signaling could be exploited to develop therapeutics for several allergic diseases, including asthma. Since isatin is known to inhibit NPRA signaling, in this study we made an attempt to find out the prophylactic role of isatin in a mouse model of ovalbumin induced allergic asthma. Methods: Four to six week old BALB/c mice (n = 4) were sensitized, treated with different doses of isatin (50,100,200mg/Kg) along with soluble chitosan intranasally and challenged with 50µg chicken egg ovalbumin (Ova). Control mice were treated with the soluble chitosan. Inflammatory cell counts were carried out on bronchoalveolar lavage fluid (BALF). Airway hyper reactivity was measured using Buxco Plethysmography. Lung histology was analyzed by hematoxylin and eosin (H & E) staining. Results: Mice treated with isatin at all doses showed a significant decrease in number of total cells and significant reduction in eosinophil count in BAL fluid (p < 0.001). Analysis of lung sections showed that isatin treatment caused substantial reduction in peribronchiolar cellular infiltration and mucus content in epithelial cells compared to untreated group. Airway hyperreactivity resistance was decreased in isatin treated group compared to control group but this did not reach statistical significance. Conclusion: Together, these results show that isatin is capable of inhibiting inflammation in asthma in this mouse model.

Research supported by: Joy McCann Culverhouse endowment to Division of Allergy and Immunology and Mabel and Ellsworth Simmons Professorship to SSM. Ruckmani Kandasamy is supported by BOYSCAST fellowship by the Ministry of Science and Technology, Government of India.

Abstract #: C-10 Presented by: Joerg Karolat, PhD, Faculty Reduced GSH Trafficking in Asthmatic Cells Correlates with High Oxidative Stress Joerg Karolat, Krishna Harohalli¹, Nicole Solana¹, Jodi L Kroeger², Stuart M Brooks¹ 1 USF 2 Moffitt Cancer Center, Tampa, USF COPH, Department of Environmental & Occupational Health Keywords: Glutathione, Asthma, oxidative stress Objective: Asthma is a chronic disease in which the airways constrict and develop inflammation in response to a variety of triggers (allergens and cellular oxidative stress).Using a 3D cell culture model we investigated the effect of oxidative stress that occurs in normal and asthmatic cell at the air-lung interface. Methods: The 3D Respiratory Mucosa Model Glutathione concentration by HPLC Fluorescent labeling Cellular transport of Hoechst dye Cytokine expression by Cytometric Bead array Oxidative stress(TBARS) Microscopy Results: The internal concentration in asthma cells is higher than the control, whereas the amount of GSH in the epithelial lining fluid is lower.The data showed reduced transport efficiency in asthmatic cells for Hoechst 33342 and a higher basal level.Measuring the malondialdehyde concentration with TBARS as an indicator for oxidative stress, revealed a higher amount of MDA in the apical wash of asthmatic cells.Both cell types expressed high levels of IL-8 and IL-6.The level of IL-6 increased slower in asthmatic cells than in normal cells whereas the IL-8 levels were higher in normal cells. Conclusion: The hypothesis under investigation is based on the assumption that asthma is triggered by environmental exposure in certain individuals with insufficient defense against oxidative stress.Glutathione data from HPLC showed decreased levels in ELF but a higher intracellular level compared to normal cells.Data from asthmatic cells signify a high level of oxidative stress and a low level of antioxidant GSH from the apical wash of 3D cells.The inhibition of glutathione synthesis by incubation with BSO exaggerated the lipid oxidation in these cells, supporting our theory about the importance of glutathione synthesis and its transport. Research supported by: SIPAIID

Abstract #: C-11 Presented by: Xiaoyuan Kong, MD, Postdoc Bone Marrow Derived Stem Cells Reduce Lung Inflammation in a Mouse Asthma ModeL Xiaoyuan Kong,Shijie Song, Xiaoqin Wang, Wilson Xu, Shawna Shirley, Richard Lockey, Shyam S. Mohapatra USF COM, Department of Internal Medicine Keywords: Bone Marrow, Stem Cell, Lung, Asthma Objective: The role of adult stem cells in repairing of lungs damaged by chronic inflammation has not been investigated. Since stem cells give rise to progenitor cells, it was reasoned that bone marrow stem cells (BMSCs) may play a important role in modulating inflammation and regional immunity of the lung. The role of BMSCs was examined in an ovalbumin (OVA)-sensitized and challenged mouse asthma model. Methods: BMSCs were collected from non-allergic 6-8 week old C57BL/6-EGFP-transgenic mice expressing enhanced green fluorescent protein (EGFP) and injected I.V. into OVA-sensitized/challenged BALB/c and C57BL/6 mice and non-sensitized mice. The lungs of BMSC or mock-injected mice were examined 1 and 2 weeks post-injection for inflammation by H &E staining and for immunomodulation by serum cytokine analyses. Results: Lung sections stained with anti-EGFP show that the asthmatic mice have a substantially high number of EGFP positive BMSCs in their lungs compared to naïve mock controls. Both syngeneic (C57BL/6) and allogeneic (BALB/c) transfer of BMSCs results in the substantial decrease of lung inflammation in asthmatic mice compared to mock controls, as revealed by infiltrated inflammatory cells and goblet cell hyperplasia. The level of serum IL-12 is also higher in the BMSC-injected group, while IL-10 and IL-13 are significantly lower, compared to controls. Conclusion: These results demonstrate that BMSCs from non-sensitized mice can reduce lung inflammation and alter cytokine levels when injected into OVA-sensitized mice.

Abstract #: C-12 Presented by: Guoqing Liu, MD, Postdoc Activation of Human Mast Cells via Natriuretic Peptide or IgE Receptor Signaling Involves Differential Expression of Inflammatory markers and SERT1 Guoqing Liu, M.C.Glaum, J.Wang, R.F.Lockey and S.S. Mohapatra USF COM, Department of Internal Medicine Keywords: Inflammation, Natriuretic Peptide, Mast Cells Objective: Mast cells mediate allergic airway inflammation through the IgE receptor, however, mast cells are also activated by immunoreactive peptides including Atrial Natriuretic Peptide (ANP), which is involved in NFkB activation and oxidative stress. In this study activation of human mast cells through the ANP receptor, was compared to that of the IgE receptor, in terms of patterns of inflammatory mediators. Methods: The human mast cell line LAD-1 and human umbilical cord blood-derived (HUCB) mast cells were primed with biotinylated human myeloma IgE. Cells were then challenged with control buffer, streptavidin (100ng/ml), ionomycin (2.5 uM) or ANP (1uM). Cell supernatants and lysates were collected at at diff time periods post-challenge and assayed for TNFSF4, TSLP, IL-10, IL-17 and CD40L by real time PCR and expression of SIRT1[Silent information regulator 2 (Sir2) proteins, sirtuins or SIRTs] measured by immunoprecipitation and western blot. Results: In LAD-1 and HUCB mast cells, IgE, ionomycin and ANP induced release of beta-hexaminidase at 30min. Mast cells stimulated through the IgE receptor and by ionomycin showed maximal expression of (TNFSF4,TSLP,CD40L) at 6 hours, however, expression of SIRT1 was decreased compared to controls. In comparison, mast cells stimulated through the ANP receptor showed increased SIRT1 expression at 6 hours that was attenuated by ANP inhibitors. Conclusion: It is concluded that activation of human mast cells through the IgE receptor and the ANP receptor yields differential expression inflammatory markers and SIRT1 expression. Research supported by: Joy McCann Culverhouse endowment to Division of Allergy and Immunology and Mabel and Ellsworth Simmons Professorship (SSM)

Abstract #: C-13 Presented by: Susan Lukas, MPH, Graduate Student Morphological Characterization of Artemisinin-Induced Dormancy in Plasmodium falciparum Susan Lukas, MPH, Dennis Kyle (a), Edward Haller (b), Matthew Tucker (a), Tina Mutka (a), Azliyati Azizan (a) (a)COPH, Global Health Department, Global Health Infectious Disease Research (GHIDR),(b)College of Medicine, Pathology Department, Microscopy Core Facility, COM USF Keywords: Plasmodium falciparium, dormancy, artemisinin,ultrastructure Objective: Artemisinin is the most potent antimalarial drug available and serves as the basis for current drug combination therapy for multidrug resistant malaria worldwide. Recent results from our lab demonstrate drug-induced dormancy on early erythrocytic stages of the parasite. Here we report on the morphological characterization of artemisinin-induced dormancy in ring stage parasites. Methods: Synchronous early ring stage parasite cultures were diluted and exposed for 6 hours to dihydroartemisinin (DHA) to induce dormancy. For fluorescent microscopy, cultures were preloaded with the vital dyes Hoechst 33342 and MitoTracker Red. Aliquots of the cultures were taken at designated intervals and processed for light microscopy, electron microscopy, or confocal fluorescence microscopy. Results: Electron micrographs taken at 24 hours post DHA challenge show parasites exhibiting nuclear condensation and cytoplasmic shrinkage characteristic of pyknotic forms seen in giemsa stained smears of dormant parasites. Confocal fluorescent microscopic analysis of prelabeled parasites show a reduction of fluorescence signal at 6 hr and loss of signal at 24 hr suggesting a change in nuclear and mitochondrial structure and function. Conclusion: These are the first reports of the unique morphological changes induced by DHA exposure in malaria parasites. These data demonstrate the utility of the DeltaVision Live Imaging System and transmission electron microscopy as tools to elucidate the morphological as well as the biochemical changes that accompany artemisinin-induced dormancy in P. falciparium. A better understanding of this process and characterization of these dormant forms may serve as a guide to more effective treatment regimens and ultimately disease resolution. Research supported by: NIAID

Abstract #: C-14 Presented by: Sarita Mendonca, BS, Staff Structural Analysis of the MAEBL Ligand Domains Sarita Mendonca, John H. Adams SF COPH, Department of Global Health Keywords: Plasmodium, MAEBL, cysteines, PAN domain Objective: Malaria is a severe disease causing significant morbidity and mortality. The disease is caused by the asexual growth of the Plasmodium species of protozoan parasites in red blood cells. Transmission of the parasite requires the completion of its life cycle in the mosquito, which is the infective stage of the parasite. A critical step in transmission involves the invasion of mosquito salivary glands. MAEBL is a chimeric protein present in the micronemes of sporozoites that invade salivary glands and has been shown to be essential to invasion. My hypothesis is that successful invasion of salivary glands requires the recognition of a specific receptor by MAEBL on salivary glands. We expect that this recognition is dependent on conformation of the M1 and M2 ligand domains of MAEBL, which are amino acid domains rich in cysteines and other residues typically important for 3D structure. The primary structures of the MAEBL ligand domains are homologous to the AMA1 ligand domain, which has shown to consist of PAN domain homologs. Methods: In order to analyze the M2 ligand domain structure, the complete domain and its subdomains M2sd1 and M2sd2 were cloned into the expression plasmid pET-21a (+) and expressed in the BL21DE3LysE bacterial strain. The recombinant proteins were extracted from inclusion bodies and purified using Ni-chelate chromatography. Results: The resulting yields were ~20mg and ~80mg for M2sd1 and M2sd2, respectively. The proteins were refolded and further purified by ion-exchange chromatography to give a final yield of 8mg for M2sd1 and 25mg for M2sd2. Conclusion: These results and MS analyses of the disulfide bond structure of the MAEBL ligand domains will be presented. Research supported by: NIH RO1 A133656

Abstract #: C-15 Presented by: Alison Montpetit, MS, Graduate Student Cigarette Smoke Condensate affects Dendritic Cell (DC) Maturation by Modulating DC-Epithelial Cell Cross-talk. Alison Montpetit, USF CON, SA Shirley, RF Lockey, SS Mohapatra USF COM, department of Internal Medicine Keywords: Dendritic Cell, Cigarette, Epithelial Cell Objective: Cigarette smoke suppresses dendritic cell (DC) function and also affects cytokine expression in epithelial cell (EC) cultures. Since smoke affects these cells simultaneously in vivo, it was reasoned that the smoke effects on DC may be modulated by its effects on ECs. Therefore, the role of cigarette smoke condensate (CSC) induced phenotypic and functional changes in DC and DC-EC cultures were compared. Methods: Confluent, monolayer EC were cultured in the presence or absence of 10 g/ml (low) or 50 g/ml (high) CSC for 12 h and poly I:C stimulated. Monocyte-derived immature dendritic cells (iDC) were then added to the EC culture or cultured alone in the presence or absence of CSC and Poly I:C for 24 h. CSC-exposed iDC were stained for viability, surface maturation markers and analyzed by flow cytometry. Cytokine levels were examined by a 14-plex bead array and endocytosis was evaluated by FITC-dextran uptake. Results: CSC exposure in both DC and EC-DC cultures have minimal effects on viability. Both doses of CSC reduce CD80, CD83, CD86 maturation markers, including CD54 (ICAM-1). DC cultures exhibit an increase in endocytosis while the EC-DC cultures show a significant decrease in endocytosis. Analysis of the cytokine profiles shows that the expression of IL1-beta, IL-6, IL-10, IL-12p40 and IL-13 increase in EC-DC cultures as compared to DC alone. When pre-stimulated with poly I:C, CSC enhances expression of IL-10, IL-12p40 and decreases expression of IL-13 in EC-DC co-cultures. Conclusion: CSC modulates EC-DC cross-talk by inducing production of cytokines that do not affect DC maturation but affect their function.

Abstract #: C-16 Presented by: Francis Ntumngia, PhD, Faculty Genetic Variation among Plasmodium Vivax Primate Isolates and the Implication for Vaccine Development Francis Ntumngia,Amy McHenry,Center for Global Health and Infectious Diseases, University of Notre Dame,; John W. Barnwell, Malaria Branch, Center for Disease Control and Prevention, Atlanta, GA; Jennifer Cole-Tobian and Christopher L. King, Center for Global Health and Disease at Case Western Reserve University School of Medicine Cleveland; John H. Adams*, Global Health Infectious Disease Research Program, USF COPH, Department of Global Health Keywords: Plasmodium vivax, Primates, DBP, Genetic diversity Objective: The Plasmodium vivax Duffy Binding protein (DBP) is vital for invasion of host erythrocytes, and the region II contains critical residues for receptor recognition thereby making this molecule a good vaccine candidate for vivax malaria. Development of a P. vivax vaccine usually requires the use of primate models, prior to testing efficacy in humans. However, little is known about the allelic diversity that occurs in the DBP in these primate isolates. This study is to determine the heterogeneity that exist in the complete gene coding for the DBP in several P. vivax isolates that are routinely used for experimental primate infections compared to the Sal 1 strain which is used as reference strain for vaccine development. Methods: PCR products from the entire gene coding for DBP in the primate isolates were cloned and sequenced. Aligned amino acid sequences were analysed for polymorphisms compared to the Sal1 strain and also for phylogenetic analysis along with human isolates from different endemic regions. Results: Our results show that the diversity of alleles from primate isolates is representative of human field isolates. Conclusion: This study re-validates primates as good models for human vaccine trials against vivax malaria. Potential DBP based vaccines must take into consideration diversity both within and outside the binding domain and also among animal models used for vaccine trials. Research supported by: This resaerch is supported by NIH grant No. R01AI064478-01A1

Abstract #: C-17 Presented by: Seungjoo Park, Undergraduate Extra-testicular Sertoli Cells May Retard The Progression Of Diabetes Seungjoo Park,J. Woodrow, L.Patrick and L. Scaglione, Dept. Pathology & Cell Biology S. Hussain, Honors College N. Dajani, Center for Aging-Neuroscience D.F. Cameron, Dept. Pathology & Cell Biology, USF CAS Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: Sertoli cells, Diabetes, Cell transplantation therapy Objective: Extra-testicular Sertoli cells (SCET) have been successfully utilized to facilitate immunoprotection of allo- and xenogeneic islet grafts transplanted for the therapeutic treatment of type-1-diabetes (T1D) and alone may retard the progression of this pathology. The objective of the study was to determine if rat SCET, transplanted into the NOD mouse with early onset diabetes, affect the progression of hyperglycemia in this animal model of T1D. Methods: NOD mice (135 mg/dl blood glucose) are transplanted (SQ) with prelabeled rat SCET and rat peritubular cells (PT), and with vehicle (sham). Blood glucose is determined (3X/wk) and blood is collected (1X/wk) for later cytokine assay. Every two weeks post-transplantation or following continued elevation of blood glucose, animals are euthanatized for the collection of pancreata and SCET grafts for the evaluation of islet pathology and viability of xenografts, respectively. Results: Preliminary results indicate that NOD mice transplanted with 3X106 rat SCET stabilized blood glucose at 138 mg/dl for 3 wks post-transplantation which was extend for more that 7 wks when transplanted with 8X106 SCET. NOD mice in the sham group attained a blood glucose of 450 mg/dl by two wks post-transplantation. Conclusion: Results indicate that the presence of rat SCET transplanted subcutaneously retards the progression of hyperglycemia as observed in this animal model of autoimmune T1D. This suggests that successful SCET-islet transplantation protocols, as previously reported, may be due in part to systemic effects of xenogeneic SCET on the progression of this autoimmue etiology. Research supported by: USF Foundation

Abstract #: C-18 Presented by: Tanmaykumar Patel, MBBS, MPH, Graduate Student Human Parainfluenza Virus 3 Infection Outbreak in a Heamatopoetic Stem Cell Transplant Unit Tanmaykumar Patel, MBBS, MPH, Kathie McDonald RN, MPH, CIC, LHRMJohn Greene Section Chief, Division of Infectious and Tropical Diseases H. Lee Moffitt Cancer Center Medicine / Internal Medicine / Infectious Diseases & International Medicine, USF Medicine / Interdisciplinary Oncology / Blood and Marrow Transplant, USF DOPH Department of Epidemiology & Biostatistics Keywords: Human Parainfluenza Virus, Immunocompromised patients, Neutropenia, Nosocomial transmission Objective: Human Parainfluenza Virus 3 (HPIV) is an important cause of morbidity and occasionally mortality among immunocompromised patients especially hematopoietic stem cell transplant (HSCT) patients. The study described risk factors and outcomes of HSCT patients who developed HPIV. The findings were used to develop an infection control protocol for the prevention of nosocomial transmission of community acquired respiratory viruses (especially HPIV). Methods: The study examined 14 cases of HPIV infection among cancer patients. Patients were selected based on their culture positive report during April to October of 2006 and their medical records were reviewed. Information about the duration of symptoms was collected from the infection control department at the cancer center. Lodging information was gathered from the local family lodge. Results: All 14 patients (10 men and 4 women) were adults (20-82 years old). Most patients received HSCT (5 allogenic and 6 autogenic) or Chemotherapy (3). The cancer diagnoses included acute myeloblastic leukemia (2), acute lymphoblastic leukemia (2), multiple myeloma (4), non-Hodgkin lymphoma (3), Hodgkin’s lymphoma (1), chronic lymphoblastic leukemia (1), and chronic myeloblastic leukemia (1). Ten patients were neutropenic during the 15-day period before or after HPIV infection. Eight patients had stayed in the family lodge prior, during or after HPIV affiliated with the cancer center. Conclusion: The nearby lodge may have resulted in nosocomial transmission of HPIV. Neutropenia may have contributed to more severe HPIV infection. Awareness of the lodge as a possible site of HPIV nosocomial transmission allowed for targeted infection control interventions, which contributed to the termination of the outbreak.

Abstract #: C-19 Presented by: Yanhua Peng, PhD, Faculty Downregulation of Adiponectin /AdipoR2 is Associated with Hepatic Inflammation and Steatosis in Obese Mice Yanhua Peng,Drew A Rideout, Steven S Rakita, Mini Sajan, Robert Farese, Min You, Michel M Murr. 1. Department of Surgery and Research, James A Haley Veterans Affairs Medical Center, USF Tampa 2. Department of Molecular Pharmacology & Physiology, USF COM Keywords: Obesity, Non-Alcoholic Fatty Liver Disease, Rodent Models Objective: Non-alcoholic fatty liver disease (NAFLD) is a common manifestation of obesity, linked to the metabolic syndrome. Adiponectin regulates fat storage, lipid oxidation, energy expenditure and inflammation. We propose that high fat diet (HFD) reduces adiponectin and induces steatosis and hepatic inflammation. Methods: C57BL mice were fed HFD (n=8) or regular chow (control; n=6) for 7 weeks to induce obesity. Body weight, liver weight and serum adiponectin were measured. Liver sections were stained for fat content. Liver homogenates were used to measure protein (immunoblotting) and mRNA (RT-PCR) of TLR4, TNF-α, SREBP-1, Adiponectin receptors (AdipoR1 and AdipoR2) in addition to nuclear phorsphorylated p65NF-κB. Gels were quantified using densitometry; p<0.05 was significant for t-tests. Results: HFD increased body and liver weight by 50% and 33%, respectively. Serum adiponectin decreased in mice fed a HFD. In liver sections, HFD increased hepatocyte fat content and ballooning. Liver AdipoR2 (protein and mRNA) decreased with HFD; however, AdipoR1 was unchanged. HFD increased (protein and mRNA) hepatic levels of TLR4, TNF-α and SREBP-1. HFD also increased activation of phorsphorylated p65NF-κB (p<0.01). P was <0.001 unless noted. Conclusion: HFD induces obesity, increases body and liver weight as well as hepatocyte fat content. Liver steatosis induced by HFD is associated with upregulation of hepatic SREBP-1 that promotes lipogenesis and fat storage. In addition, HFD is associated with upregulation of pro-inflammatory transcription factors and cytokines and downregulation of anti-inflammatory AdipoR2. Moreover, obesity-induced reduction in serum adiponectin suggests that adiponectin signaling may be the link between HFD, inflammation and NAFLD.

Abstract #: C-20 Presented by: Chris Rogers, PhD, Postdoc Role Of Hepatic Adiponectin Receptor 2 In The Protective Action Of Dietary Saturated Fatty Acids Against Alcoholic Liver Steatosis In Mice. Chris Rogers, PhD, J.M. Ajmo; X. Liang; C.Q. Rogers; M. You. USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: adiponectin receptor; alcoholic liver steatosis; fatty acid; hepatoma cells Objective: Our previous work has demonstrated that the protective action of saturated fat against alcohol-induced fatty liver was associated with increased circulating adipose-derived adiponectin in mice. Methods: In the present study, we further examine the effects of dietary fat on mRNA and protein levels of hepatic adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2) in chronic ethanol-fed mice. Results: Consistent with reports by others, mouse liver expressed a greater (~2-fold) quantity of AdipoR2 compared to AdipoR1. Ethanol (E) feeding of either a high polyunsaturated fat (PUFA) or a high saturated fat (HSF) diet did not cause significant changes in levels of hepatic AdipoR1 in mice. However, the mRNA and protein levels of AdipoR2 were significantly increased by the HSF+E diet compared with the control mice receiving the HSF diet, whereas the mRNA and protein expression levels of hepatic AdipoR2 were significantly suppressed by the PUFA+E diet. Furthermore, treatment of rat hepatoma H4IIEC3 cells with saturated fatty acid (palmitic acid) in the presence of ethanol robustly increased the AdipoR2 mRNA expression and enhanced activity of a mouse AdipoR2 promoter. Conclusion: Taken together, our findings suggest that HSF diet-mediated up-regulation of hepatic AdipoR2 in the ethanol fed mice could be partially associated with the protective effect of the HSF diet against the development of alcoholic fatty liver. Research supported by: This study was supported by NIH/NIAAA grants AA015951 and AA013623 (to M. You).

Abstract #: C-21 Presented by: Fabian Saenz, BS, Staff The Transmembrane Isoform of Plasmodium Falciparum MAEBL is Essential for the Invasion of Anopheles Salivary Glands Fabian E. Saenz*†, Bharath Balu*, Jonah Smith†‡, Sarita R. Mendonca*†, and John H. Adams*§ *Global Health and Infectious Diseases Research, Department of Global Health, COPH USF; †Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana Keywords: MAEBL, Plasmodium falciparum, Anopheles, alternative splicing Objective: Malaria transmission depends on infective stages in the mosquito salivary glands. Plasmodium sporozoites that mature in midgut oocysts must traverse the hemocoel and invade the mosquito salivary glands in a process thought to be mediated by parasite ligands. MAEBL, a homologue of the transmembrane EBP ligands essential in merozoite invasion, is expressed abundantly in midgut sporozoites. Alternative splicing generates different MAEBL isoforms and so it is unclear what form is functionally essential. The objective of this research was to identify the MAEBL isoform required for P. falciparum (NF54) sporozoite invasion of salivary glands. Methods: We created knockout and allelic replacements each carrying CDS of a single MAEBL isoform and we infected Anopheles mosquitoes. Results: We found that only the transmembrane form of MAEBL is essential. Conclusion: This is the first P. falciparum ligand validated as essential for invasion of Anopheles salivary glands. Understanding what P. falciparum sporozoite ligands are critical for mosquito transmission will help validate targets for vector-based transmission-blocking strategies. Research supported by: NIH grant R01 A133656

Abstract #: C-22 Presented by: Tracy Sherwood, BS, Graduate Student Analysis of Cb2 Transcription Initiation in Mouse and Human B Cells T.A. Sherwood, L. Nong, M. Agudelo, C. Newton, R. Widen, and T.W. Klein. Department of Molecular Medicine, COM USF Basic Biomedical Sciences - Molecular Medicine Keywords: Cannabinoids, B lymphocytes Objective: Studies have shown that cannabinoid receptor 2 (CB2) mRNA is most abundant in mouse and human B cells. Searching of genomic databases revealed that the mouse cnr2 gene is predicted to produce two transcripts, whereas the human cnr2 gene is predicted to produce one CB2 transcript. However, from the database search we could not determine CB2 transcript initiation in B cells. Therefore, we wanted to explore CB2 transcription initiation at basal activity in B cells from mouse and human. Methods: We isolated B cells from mouse splenocytes and human PBMCs using either the mouse or human EasySep B cell enrichment kit. Cell enrichment was determined by FACS analysis using CD19 and CD3 antibodies. Total RNA was extracted from the B cells for RT-PCR employing the SMART RACE technique. All RACE products were gel purified and sent for DNA sequencing. Results: The FACS analysis demonstrated that the human and mouse B cell populations were enriched by 95 to 98%. Sequencing of the RACE products showed that mouse B cells express three CB2 transcripts utilizing three different initiation sites, whereas the human B cells express one CB2 transcript utilizing one initiation site. Alignment of the sequenced RACE products to either the mouse or human genome, along with the GenBank submitted mRNA sequences, revealed that the transcripts we isolated contained previously unidentified transcription initiation sites (TIS). Conclusion: The data show, at basal activity, that mouse splenic B cells express three cnr2 gene transcripts utilizing three TIS, whereas human B cells express one cnr2 gene transcript using one TIS. Future studies will examine the CB2 exonal usage in B cells of mice under various conditions of activation. Research supported by: Supported by NIDA grant DA019824

Abstract #: C-23 Presented by: Shawna Shirley, MS, Graduate Student Curcumin Modulates Human Dendritic Cell Function Shawna Shirley,,AJ Montpetit, RF Lockey and SM Mohapatra USF COM, Department of Internal Medicine Keywords: Curcumin, Dendritic Cell, Asthma Objective: Curcumin has been reported to have potent anti-inflammatory properties. We examined its effects on human dendritic cell maturation and function in-vitro. Methods: CD14 monocytes were isolated from human peripheral blood and cultured for 5 - 6 days in complete medium with GM-CSF and IL-4 (20ng/ml each). Cells were treated with curcumin dissolved in DMSO for 1hour and then exposed to LPS (1?g/ml) overnight. Curcumin toxicity was measured by annexin V staining and flow cytometric analysis. Cell morphology was examined by light and confocal microscopy and cytokine production was measured from the culture supernatant by multiplex bead assay (Linco). Surface maturation markers were stained with fluorescently labeled antibodies and analyzed by flow cytometry. Endocytosis assays were carried out using Alexa647-conjugated dextran (Invitrogen). A mixed lymphocyte reaction was carried out to test DC function. T cell proliferation was measured by analysis of CFSE dye by flow cytometry. Results: Curcumin was toxic to cells at high concentrations (over 100µM). Stimulated DCs cultured with curcumin produced significantly lower levels of the cytokines IL-12, IL-10, TNF-? and IL-1? when compared to stimulated controls. The expression of surface markers CD86 and CD54 were lower in DCs cultured with curcumin than in the stimulated controls but CD11c, HLA-DR, CD83 and CD80 expression was unaffected. Curcumin DCs show reduced endocytosis compared to non-stimulated controls. In mixed lymphocyte reaction DCs cultured with curcumin do not induce proliferation of CD4 T cells. Conclusion: Taken as a whole, these results suggest curcumin can modulate human dendritic cell function.

Abstract #: C-24 Presented by: Aimee Signarovitz, MPH, Graduate Student Quantification of Vascular Endothelial Growth Factor in Endothelial Cells Infected with Dengue virus Aimee Signavotitz (a,b), Kelly Fitzpatrick (a,b), Mark Sweat (a,c), Lillian Stark (a,b), Deno Kazanis (a,b), and Azliyati Azizan (a,c). Global Health Department, USF COPH, Florida Department of Health, Bureau of Laboratory, Tampa, Global Health Interdisciplinary Research (GHIDR), Tampa, FL Keywords: dengue, virus, Luminex, VEGF, and IL-2 Objective: Infection by dengue virus can cause the life-threatening illness Dengue Hemorrhagic Fever (DHF). Increased vascular permeability mediated in part by upregulated cytokines and growth factors, is the main cause of circulatory failure in DHF patients. The aim of this in-vitro study was to investigate the ability of dengue virus serotypes 1, 2, 3 and 4 (DENV-1, -2, -3, and -4)) to infect and induce increased production of vascular endothelial growth factor (VEGF) and interleukin-2 (IL-2) in a human pulmonary endothelial cell line (HPMEC-ST1.6R). Methods: The level of VEGF and IL-2 within conditioned media of DENV-1, -2, -3 and -4-infected HPMEC-ST1.6R, at specific time points, was quantified using a microbead immunoassay (MIA). The MIA assay allowed quantification of cytokines at the pg/ml level. Evidence of infection in DENV-inoculated HPMEC-ST1.6R was then observed through the presence of a cytopathic effect and an immunofluorescence assay. Results: Increase of VEGF level over time in DENV-infected cells was found to be greater than that of IL-2. A time-dependent pattern of increasing VEGF level was observed, and remained consistent over several days post-infection. In contrast, IL-2 levels did not follow the same pattern. Conclusion: Data from this study would suggest a correlation between time-dependent increases in VEGF levels and DENV-infection. This pattern is consistent within all four dengue serotypes. These data supports our previous findings and suggests that VEGF may be involved in in-vivo processes leading to vascular leakage, which play a role in the immunopathogenesis of DHF. Research supported by: Global Health Department, COPH, USF, Tampa, Florida

Abstract #: C-25 Presented by: Shelly Smith, MPH, Graduate Student Copy Number And Expression Of pfmdr1 In Artemisinin-Resistant Plasmodium Falciparum Shelly Smith, MPH Azliyati Azizan (a,b), Susan Lukas (a,b), Tina Mutka (a,b), Matthew Tucker (a,b), and Dennis Kyle (a,b). (a) Global Health Department, USF College of Public Health, Tampa, Florida. (b) Global Health Infectious Disease Research (GHIDR), USF Keywords: malaria, Plasmodium falciparum, artemisinin, mdr1, PCR Objective: The emergence of Plasmodium falciparum strains to various antimalarial drugs presents a great challenge for effective malaria control. Resistance to the efficacious artemisinin drugs has not yet been documented in the field, but has been produced experimentally in our lab. The amplification of pfmdr1 is involved in resistance to multiple, structurally unrelated drugs in P. falciparum.We hypothesized that amplification and increased gene expression of pfmdr1 and other transporters, were the basis of resistance in the laboratory strains. Methods: Total DNA and RNA extracts were prepared from stable laboratory-adapted artemisinin resistant P. falciparum. Susceptibility to artemisinin and control drugs was assessed. A SYBR Green real time RT-PCR assays (MX300P, Stratagene) using primers specific for pfmdr1 and other transporter candidate genes were performed to determine changes in gene expression and copy number. Results: Using the ΔΔCt method, we observed an increase in pfmdr1 copy number from a single copy in parental strain W2 to 2-3 in the resistant strains. Normalized to actin, ΔΔCt RT-PCR values for pfmdr1 revealed that relative mRNA level was increased for resistant strains, which is consistent with the increase in pfmdr1 amplification. Conclusion: A real time PCR assay was used to assess gene copy number and changes in gene expression for pfmdr1 and other transporter genes. We found that increased expression and amplification of pfmdr1 is associated with artemisinin resistance in some lab generated lines. We are optimizing these molecular assays so as to achieve our overall goal of identifying novel genetic markers of dormancy and resistance in P. falciparum, which can be used for molecular epidemiology studies. Research supported by: NIAID

Abstract #: C-26 Presented by: Matthew Tucker, MS, Graduate Student Genetic Analysis of Artemisinin and Artelinic Acid Resistant Plasmodium Falciparum Matthew Tucker, Jennifer Peters 2, Martin Nau 3, Zhinning Wang 3, Qin Cheng 4, Maryanne Vahey 3, Azliyati Azizan 1, and Dennis E. Kyle 1 1 USF COPH Department of Global Health 2 Queensland Institute of Medical Research, Brisbane, Australia 3 Walter Reed Army Institute of Research, Rockville, MD 4 Australian Army Malaria Institute, Enoggera, Australia Keywords: Artemisinin Plasmodium falciparum genetic resistance

Objective: Artemisinin (QHS) drugs are the basis for current efforts to eradicate disease. These drugs are rapidly acting and represent the sole class of antimalarials for which clinical resistance has not been reported. therefore it is critical to investigate potential resistance mechanisms. We have developed resistant parasites and are using these lines to determine the molecular basis of QHS resistance. Methods: Discontinuous exposure to artelinic acid (AL) or QHS in vitro produced AL and QHS resistant progeny in three P. falciparum lines. Microarray studies were performed on clone W2 and its progeny that are resistant to QHS (40 ng/mL) or AL (80 ng/mL) to identify genes that may be associated with QHS and AL resistance. Results: Analysis of microarray data found >40 probesets that were significantly up or down regulated (p<0.001). Of these differentially expressed genes, the most significant was PFE1050w, which encodes a S-adenosyl-L-homocysteine hydrolase. Prior candidate gene studies identified pfmdr1 amplification in resistant progeny, and the microarray data confirmed these findings. Interestingly, pfmdr2 was downregulated in resistant progeny as compared to the parent clone. These data also identified multiple transporters that were significant (p<0.05), with the most significant being PF11_0466. Conclusion: Microarray analysis of induced QHS resistant malaria identified novel candidate genes for QHS resistance mechanism studies. The interesting trend for down-regulation of transporters may be due to novel SNPs that reduce binding affinity for probe sets representing these genes. Further analysis into the importance of these nucleotide differences will focus on absolute SNP identity and real-time PCR to correlate copy number and expression data. Research supported by: NIAID

Abstract #: C-27 Presented by: Jia-Wang Wang, PhD, Postdoc Role of MicroRNAs in the Pathogenesis of Asthma Jia-Wang Wang,W Zhang, X Wang and RF Lockey, SS Mohapatra USF COM, Department of Internal Medicine Keywords: Asthma, MicroRNA, TH1, TH2 Objective: Successful immunotherapy appears to result in increased number of T regulatory cells (Treg), which inhibit activation of Th2 and Th1 cells. This suggests that the lack of Treg cells promotes asthma pathogenesis. We have been investigated the expression of non-coding microRNAs (miRNA) regulating asthma (miRRAs) to understand the initial molecular events in the pathogenesis of asthma. Methods: A 684 oligonucleotide library of antisense to human and mouse mature miRNAs was used to identify miRRAs, The expression of miRNAs was examined in splenocytes and purified CD4+CD25+Treg and non-Treg CD4+ cells from asthmatic, naïve mice and asthma-tolerant NPRA-/- mice. The dot blot results were confirmed by real-time PCR. Results: A comparison of the miRNA expression profile shows that miRNAs are globally decreased in asthmatic versus naïve mice (up to 140 times), and Treg versus non-Treg CD4+ cells are increased in (up to 150 times). Of these, the expression of 3 miRNAS, miRRA-1, miRRA-2 and miRRA-3 is significantly decreased in asthmatic versus naive mice (1.7, 40 and 32 folds, respectively), but increased in Treg versus non-Treg CD4+ cells (2, 6 and 2 folds, respectively). The NPRA-/- versus wild type mice have an increased percentage of Treg cells show an increased expression of miRRA-1, miRRA-2 and miRRA-3 by 2, 11 and 1.8 folds, respectively compared to wild type mice. Conclusion: These results suggest that miRRAs may play a role in the pathogenesis of asthma and may regulate T cell polarization.

Abstract #: C-28 Presented by: Xihai Wang, MD, Postdoc Intravesical Antibodies to Cd74 (Binding Protein For Mif) or Glucose Regulated Protein 78 (Grp78) Prevent Substance P Induced Inflammatory Changes in the Bladder Xihai Wang,P.L. Vera, K.L. Meyer-Siegler USF COM, Department of Surgery Keywords: Macrophage migration inhibitory factor(MIF) Urothelium Susbstance P CD74 GRP78 Objective: Macrophage migration inhibitory factor (MIF) is expressed in the urothelium and upregulated during inflammation. MIF is released intravesically complexed to alpha1-inhibitor-3. We examined the effect of blocking CD74 (binding protein for MIF) and GRP78 (receptor for alpha1-inhibitor-3) both of which are upregulated during inflammation. Methods: Male rats were anesthetized, the bladders were isolated. Rats received saline (i.p.) and saline intravesically or Substance P and saline, anti-D74, anti-GRP78, both anti-CD74 and anti-GRP78, and nonspecific IgG. After 1h, the intravesical fluid and the bladders were removed and the urothelium was collected. MIF were measured using ELISA. Inflammatory cytokine production was measured using a cytokine array. Bladder histology were used to assess edema and inflammation. Results: Susbstance P decreased urothelial MIF levels and increased intravesical MIF levels indicating MIF release from the urothelium. Anti-GRP78 prevented this effect while anti-CD74 and anti-CD74/anti-GRP78 actually increased the amounts of urothelial MIF. Bladder edema scores, while markedly increased with SP and saline or IgG treatment, decreased with anti-CD74 and/or anti-GRP78 treatment. Inflammatory cytokine production was markedly increased with SP treatment and anti-CD74 or anti-GRP78 prevented such changes. Conclusion: Blockade of CD74 or GRP78 prevent Substance P induced inflammatory changes in the rat bladder. Thus, acting through two different cell-surface receptors, MIF and/or MIF-complexes activate an inflammatory cascade. These findings suggest that cell-surface CD74 and GRP78 play an active role in bladder inflammation in the rat.

Abstract #: C-29 Presented by: Jane Woodrow, BS, Graduate Student Fate of IV Injected Xenogeneic Sertoli Cells Jane Woodrow,,L.Patrick and L. Scaglione, Dept. Pathology & Cell Biology; S. Park and S. Hussain, Honors College; N. Dajani, Center for Aging-Neuroscience; A. Kumar, Dept. Int. Medicine; D.F. Cameron, Dept. Pathology & Cell Biology, USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Sertoli cells, transplant, lung, nanoparticles Objective: Allo- and xenogeneic cell grafts avoid immune rejection when co-transplanted with extra-testicular Sertoli cells (SCET). Mechanisms by which SCET immunoprotect cells are not yet defined. Recently, Shamekh et al (2006) reported that rat SCET injected IV into the mouse host significantly enhanced the survival of xenogeneic rat skin grafts by the induction of donor specific induced tolerance. The objective of this study was to determine the fate of IV injected rat SCET in the mouse host Methods: Rat SCET pre-labeled with DiI or lung-adherent chitosan nanoparticles labeled with FITC were injected into the mouse host. Immediately thereafter, tissues including lung, spleen and thymus were snap frozen in liquid nitrogen, fixed with 4% paraformaldehyde or homogenized for qualitative and quantitative detection of SCET and nanoparticles. Results: Intact pre-labeled SCET were not observed in any of the tissues examined. However, in the lung only, SCET debris was apparent. Labels from cellular debris and nanoparticles were observed in lung parenchyma and, with stacked confocal images, in pulmonary macrophages. Additionally, tissues from transplanted mice were assayed by HPLC for FITC to quantify the distribution of nanoparticles and, therefore, localization of the cells. Conclusion: Results indicate that pre-labeled rat injected (IV) in the mouse become entrapped in the host’s lung. This suggests that SCET induced donor specific immune tolerance is initiated in this tissue, and that the immunoprotection of xenogeneic islets, as observed in successful SC-islet co-transplantation protocols, may be the result of systemic immunomodulation not requiring the presence of SCET at the graft site. Research supported by: USF Foundation

Abstract #: C-30 Presented by: Weidong Xu, PhD, Faculty Modulation of Lung Inflammation by Vessel Dilator, an N-Terminal Natriuretic Peptide, in a Mouse Model of Allergic Asthma Weidong Xu,Xiaoqin Wang, , Dongqing Chen, Xiaoyuan Kong, Richard F Lockey, Shyam S Mohapatra USF COM Department of Internal Medicine Keywords: Lung, Inflammation, Natriuretic Peptide Objective: Previous studies show that a novel N-terminal natriuretic peptide, NP73-102 (amino acids 73-102), has significant bronchoprotective and anti-inflammatory effects. These results led to the hypothesis that other N-terminal hormone peptides also possess bronchodilatory and anti-inflammatory properties. Therefore, In this study, the activity of vessel dilator [(VD), amino acids 31-67)] was examined in a mouse model of allergic asthma. Methods: A549 cells were transfected with pVD pr pVAX-1, and the anti-inflammatory effects of VD examined by western blot for ERK1/2 and NF?B. VD effect on NPRA expression was examined using NPRA-promoter linked luciferase reporter constructs. Balb/c mice were sensitized and challenged with ovalbumin and administered nanoparticles expressing pVD or pVAX1 (vector control). Differential cell counting in bronchoalveolar lavage fluid and histopathology staining of lung sections was performed 4 days after pVD administration. Results: pVD overexpression significantly (p<0.001) decreases NPRA in A549 cells. A549 cells transfected with pVD show a significant reduction in the activity of NFkB and ERK1/2 compared to controls. Mice with ovalbumin-induced asthma versus control treated with nanoparticles carrying pVD show a substantial decease in lung inflammation, airway hyperreactivity and number of BAL eosinophils. Conclusion: These results show that VD peptide significantly attenuates allergic inflammation and airway hyperreactivity, VD may be a novel ew therapeutic agent for asthma. Research supported by: NIH (5R01-HL71101-01A2), VA Merit Review Award and Joy McCann Culverhouse and Mabel & Ellsworth Simmons Endowments

Abstract #: C-31 Presented by: Weidong Zhang, MD, Faculty Mechanism of Atrial Natriuretic Peptide Receptor (NPRA)-mediated Induction of Tolerogenic Dendritic Cell (DC) Weidong Zhang,X Cao, D Chen, X Kong, G Hellermann, J Wang, S Boyapalle, RF Lockey and SS Mohapatra USF COM Department of Internal Medicine Keywords: Dendritic Cell, NPRA, Asthma Objective: Atrial natriuretic peptide (ANP) ANP polarizes human DCs toward a Th2-promoting phenotype through NPRA, however, the precise mechanisms underlying NPRA-mediated immunity remain unclear. The role of NPRA signaling in modulating DC function was examined using ANP and another NPRA-signaling inhibiting peptide NP73-102 in mouse models and human monocyte-derived DCs. Methods: The role of ANP and NP73-102 in respectively, inducing Th2 and Treg cells was investigated using in vivo gene expression and adaptive DC transfer in wild type C57BL6 mice. Human monocyte-derived DCs were used to investigate downstream molecules and the protein interactions among these molecules involved in NPRA signaling pathway using RNA interference coupled with immunoprecipitation and western blotting. Results: Adaptive transfer of bone marrow –derived DCs from wild type to NPRA-/- mice indicate that ANP polarizes DCs to promote Th2-dominant response. in contrast, NP73-102, which inhibits NPRA primes DCs to induce regulatory T (Treg) cells as evident from the percentage of CD4+ CD25+ cells and FoxP3 staining. In cultured human DCs, NPRA binds to TLR-2, SOCS3 and STAT3 and affects induction of IL-6, IL-10 and TGF-?, not IL-17. SOCS3 expression is controlled in a MyD88-dependent manner. Also, down-regulation of SOCS3 and TLR2, but not STAT3, affects NPRA expression. Conclusion: These results demonstrate that TLR2, and SOCS3 are key players in integrating ANP-NPRA signaling with innate immunity and provide insight into how NP73-102 inhibition of NPRA by affects its downstream signaling which promotes the tolerogenic phenotype of DCs.

Session “A”

BIOMEDICAL ENGINEERING AND DRUG DISCOVERY

CANCER BIOLOGY

CARDIOVASCULAR SCIENCES

COMPUTATIONAL BIOLOGY AND TECHNOLOGY

ENVIRONMENTAL AND GLOBAL HEALTH

FAMILY AND GENERAL HEALTH

NEUROSCIENCE

OCCUPATIONAL AND WORK PLACE ISSUES

OTHER CLINICAL STUDIES AND CASE REPORTS

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Abstract #: A-32 Presented by: Audrey Buttice, MS, Graduate Student Improving Water Quality with Green Chemistry Audrey Buttice, MS,,Kathryn Bailey (2), Joyce Stroot (3), Daniel Lim (3), Peter Stroot (2), Norma Alcantar (1), (1) Chemical Engineering Department, (2) Civil and Environmental Department, (3) Advanced Biosensors Laboratory-Department of Biology USF College of Engineering Chemical Engineering Keywords: Cactus Mucilage, Water Sanitation, Opuntia ficus-indica, Bacillus Cereus, E. Coli Objective: In many countries the ability to increase the quality of health is severely hindered by waterborne pathogens resulting from poor water storage. Although focus worldwide has been directed to water sanitation, many issues have been encountered dealing with maintenance and local acceptance. We aim to purify water, while avoiding these issues, by studying and utilizing a mucilage compound, extracted from the Opuntia ficus-indica, a cactus common to Mexico, as a form of green chemistry used to increase settling rates of waterborne contaminants. Methods: Experiments include the extraction of three different fractions of mucilage from cactus pads using simple separation techniques. The growth and maintenance of E. coli and Bacillus Cereus bacteria is preformed using standard microbiology procedures. Column tests are then utilized to examine how the settling rate of bacteria, suspended in water, is affected by the addition of cactus mucilage. Before and after the tests are run, bacteria counting techniques are applied for analytical comparison. Results: In the Bacillus columns the reaction with the addition of mucilage was immediate and flocculation was complete in approximately five to ten minutes with removal rates of up to 97%. Similar flocculation over time has been witnessed in E. coli columns. Conclusion: The use of Cactus mucilage as a flocculating agent could greatly increase the quality of life and health in many countries that struggle with contamination. Not only is it a natural substance, but it is of low cost, easy to use and can be readily obtained and processed. Its implementation also avoids many of the social and structural tribulations witnessed with current relief attempts granting it the ability to reach a large range of populations.

Abstract #: A-33 Presented by: Richard Cross, PhD, Graduate Student Quinolones as Novel Chemotypes Targeting Atovaquone Resistant Malaria Parasites Richard Cross, PhD, Tina Mutka (2), Dennis E. Kyle (2)*, Roman Manetsch (1)* (1) Department of Chemistry, College of Arts and Science, USF (2) Department of Global Health, College of Public Health, USF Keywords: Malaria, Plasmodium falciparum, Multi-Strain Resistance, Quinolone Objective: Malaria is the most important parasitic disease of man, with over 2 million deaths per year and > 300 million cases annually. New drugs are urgently required to combat this deadly disease. Due to a limited number of chemotypes and documented resistance to the available drugs, there is renewed international effort aimed to discover and develop new leads with potential to prevent or treat malaria. Herein, we report the discovery and development of quinolone compounds displaying excellent antimalarial activity. Methods: The specific hurdle for developing the quinolone chemotype is the potential for cross resistance with atovaquone, a currently used antimalarial drug. Atovaquone was developed as a single agent, but later was combined with proguanil to prevent the emergence of resistance resulting from mutations in cytochrome b. We prepared and tested a focused library of quinolone compounds against atovaquone-susceptible and -resistant P. falciparum. Results: Our preliminary data demonstrate the ability of compound RM-MC2 to prevent growth of P. falciparum at low nM concentrations in vitro. In addition to activity against the erythrocytic stages associated with symptoms of malaria, the class has potential as preventative drugs due to efficacy against liver stage organisms. Conclusion: In this study, quinolones have been shown to be a potential antimalarial chemotype displaying excellent activity against atovaquone resistant P. falciparum strains. Currently, we are in progress of preparing quinolone derivatives to obtain solid structure activity relationships and to deepen the understanding of the mechanism of action of this novel chemotype. Research supported by: University of South Florida

Abstract #: A-34 Presented by: Dawn Fox, PhD, Graduate Student Removal of Arsenic from Drinking Water using Cactus Mucilage Dawn Fox, PhD,,Thomas Pichler (Department of Geology), Daniel Yeh (Department of Civil and Environmental Engineering), Norma Alcantar (Department of Chemical Engineering), USF College of Engineering Chemical Engineering Keywords: arsenic, cactus mucilage, drinking water, Opuntia ficus-indica Objective: People in many parts of the world are exposed to arsenic levels greater than 10 ppb. This is the maximum allowable concentration for drinking water recommended by the World Health Organization and enforced by the US EPA. Contamination is mainly due to erosion of natural arsenic deposits into the groundwater supply. The conventional treatment methods typically require centralized treatment facilities which may be inaccessible to poor rural communities on account of being expensive, energy intensive and requiring highly technical maintenance. In previous work, cactus mucilage was shown to flocculate suspended solids and remove arsenic from water. Cactus mucilage is an extract from the Opuntia ficus-indica (also known as Nopal and Prickly pear cactus). In the current study, we are investigating the mechanism by which the mucilage removes arsenic from water, with the aim of optimizing the process. Methods: Batch kinetic and column-depth experiments were performed in which the mucilage was contacted with aqueous arsenic solutions. The metal concentration was detected with hydride generation atomic fluorescence spectroscopy. Results: Our results suggest that a complex is formed between the sugars and carbohydrates compounds of the cactus mucilage and the solvated arsenic ions, followed by a phase separation of such complex. Conclusion: It is envisaged that the technology developed from cactus mucilage will solve the arsenic problems facing poor rural communities on the basis of being relatively inexpensive and easy to use, and requiring little or no electrical energy to operate.

Abstract #: A-35 Presented by: Arun Babu Kumar, PhD, Graduate Student Development of a Labeling Probe for the Discovery and Identification of Saccharide-Binding Proteins Arun Babu Kumar, PhD,,Roman Manetsch* College of Arts and Sciences, Department of Chemistry, USF Keywords: Saccharides, Lectins, Photoaffinity Labeling, Proteomics Objective: Proteins that interact with carbohydrates in a non-covalent fashion occur widely in nature. Such proteins, which have come into the forefront of biological research in recent years, belong to the class of lectins. We discuss the design and development of a versatile chemical tool for the selective labeling of carbohydrate-binding proteins. The long term goal of our research is to develop versatile labeling probes and methodologies, which are applicable for studies targeting entire proteomes. Methods: Photoaffinity labeling is a biochemical approach that allows a ligand to form a covalent bond with the target receptor upon irradiation. Despite progress in the design of photoaffinity probes, experiments in which labeled peptides or amino acids have been identified are relatively limited. Herein we introduce a novel class of multifunctional photoaffinity probes, which guarantee the highest possible affinity combined with a straight forward purification procedure by a fluorous tag. Results: Herein we report our synthetic efforts for the preparation of the multifunctional photoprobe. In a proof of concept, we will test our labeling probe on Concanavalin A (Con A), a commercially available lectins. Con A is probably one of the best known saccharide binding proteins, and therefore ideally suited for this purpose. Conclusion: Although our research endeavors are still ongoing, we speculate that our proposed enrichment of labeled protein fragments from non-labeled via a fluorous tag has potential to accelerate the discovery & identification of unkown saccharide binding proteins. If a particular group of proteins can be isolated from the proteome mixture, it would reduce the complexity involved in the subsequent 2D-gel electrophoresis. Research supported by: University of South Florida

Abstract #: A-36 Presented by: Thomas Lendrihas, MA, Graduate Student Altering the Substrate Specificity of 5-Aminolevulinate Synthase to accept a More Hydrophobic Substrate Using Rational Design Thomas Lendrihas,,Junshun Zhang¶, Gregory A. Hunter¶ and Gloria C. Ferreira¶,§,# ¶Department of Biochemistry and Molecular Biology, College of Medicine and §H. Lee Moffitt Cancer Center and Research Institute, and #Department of Chemisty, USF COM Basic Biomedical Sciences - Molecular Medicine Keywords: pyridoxal, heme, transient kinetics, enzymology Objective: 5-Aminolevulinate synthase (ALAS), a pyridoxal 5'-phosphate-dependent enzyme, catalyzes the first, and key regulatory step of the heme biosynthetic pathway in non-plant eucaryotes and the ?-subclass of purple bacteria. This reaction involves the condensation of glycine and succinyl-Coenzyme A (CoA) to produce 5-aminolevulinate (ALA), CoA and carbon dioxide. Methods: The x-ray crystal structure of ALAS from Rhodobacter capsulatus reveals that the alkanoic acid tail of succinyl-CoA is coordinated by a conserved arginine and a threonine. The threonine is hydrogen-bonded to both the substrate succinyl-CoA and the conserved arginine. With this in mind, we set out to rationally design an enzyme that would select for alternative acyl-CoA substrates of greater hydrophobicity. Results: When the corresponding arginine residue in murine erythroid ALAS (Arg-85) is mutated to leucine the catalytic efficiency for octanoyl-CoA increases by 66-fold. Similarly, the double mutated variant, when both the threonine (Thr-430) and arginine residues are mutated to valine and leucine, respectively, the relative catalytic efficiency for butyryl-CoA is increased 35-fold. Further, while the formation of a quinonoid intermediate during the reaction of wild-type ALAS with glycine and succinyl-CoA can be easily monitored using rapid scanning stopped-flow spectroscopy, the corresponding quinonoid intermediate is either not detected or more weakly detected in the variant-catalyzed reactions. Conclusion: We report here the successful construction of ALAS variants with altered substrate specificity using rational design. Together, our data highlight that Arg-85 and Thr-430 are important for securing the correct organizational state of the active-site/substrate complex. Research supported by: R01DK063191-02

Abstract #: A-37 Presented by: Shikha Mahajan, PhD, Graduate Student Synthesis of Azido-Adenine-Ribose Derivatives: Potential Activity Based Protein Profiling Probes Shikha Mahajan,,David Merkler*, Roman Manetsch* University of South Florida College of Arts and Sciences Chemistry Keywords: Activity Based Protein Profiling, Adenine, Biotin, Photoaffinity Labeling Objective: Adenine nucleosides and nucleotides (AdoR's) are key molecules in virtually every metabolic and cell signaling pathway. Thus, AdoR-dependent proteins correlate to many human diseases including cancer, cardiovascular diseases, diabetes, and obesity. Activity based protein profiling (ABPP) is an emerging powerful technique to study protein functions on a proteome-wide scale and synthesis of these ABPP probes is designed based on considerable knowledge of their enzymology to label a mechanistically related family of enzymes. We aim at synthesizing a panel of AdoR derivatives and the validation of the use of our AdoR-specific probes against a set of purified AdoR-dependent proteins. Methods: Herein, we report our efforts to synthesize biotinylated azido-AdoR analogues for the study of AdoR-dependent proteomes. The design of these AdoR probes includes a photolabile azide moiety for covalent attachment of the probe to the target proteins as well as a biotin moiety for the isolation of the probe-protein conjugates using avidin affinity chromatography. Results: We designed a convergent synthetic route for the preparation of the AdoR probe starting from commercially available inosine and biotin. Additionally, we aim at synthetic procedures leading to an intermediate, which can be converted into various AdoR derivatives. Conclusion: Although our research endeavors are still ongoing, we plan to synthesize biotinylated adenine derivative intermediate and analogs thereof with extended linker lengths. Additionally, we will prepare ABPP probes for some biologically relevant targets known to bind S-adenosylmethionine, S-adenosylhomocysteine, 5’-methylthioadenosine, AMP, ADP, coenzyme A, and others. Research supported by: University of South Florida

Abstract #: A-38 Presented by: Joshua Schumacher, MS, Graduate Student Sensitivity Enhancement of MALDI Mass Spectrometry Measurements Using H2O-Insoluble Matrix Compounds Joshua Schumacher,,John M. Koomen, Moffitt Cancer Center, Rudy Schlaf, Department of Electrical Engineering, USF College of Engineering Keywords: mass spectrometry, sensitivity enhancement, peptides Objective: Matrix-assisted laser desorption/ionization (MALDI) is a soft ionization technique used in mass spectrometry of large molecules including biomolecules. This preparative technique requires a proton-donating matrix to be co-crystallized with the analyte. This is achieved by mixing solutions containing the matrix and the analyte, which are drop-deposited onto a sample plate. As the solvent evaporates, a crystalline deposit is formed. After introduction into the mass spectrometer, the sample is desorbed with a focused UV laser beam. Ionization of the analyte occurs by proton transfer from matrix to analyte during desorption. One of the issues of such measurements is that the crystalline deposit is often irregular making it difficult to obtain a good signal. Furthermore, the laser spot is smaller than the deposit, making it necessary to interrogate various spots until a good signal is found. The objective of the presented research is the development of a technique, which, similarly to anchor plates available for water-soluble matrix compounds, enables droplet anchoring and lateral deposit concentration for analyte/matrix mixtures of non-water soluble compounds. Methods: The developed sample deposition technique is based on nanoscale surface modification of the sample plate, resulting in a localized deposition of the matrix. Results: The presented results demonstrate well-defined analyte/matrix concentration into spots of about 150 micron diameter (equal to the laser spot diameter), and resulting signal-to-noise improvements in the range of 3x to 50x, depending on experimental parameters. Conclusion: The reproducibility and signal-to-noise ratio/sensitivity of MALDI measurements based on non-water soluble matrix compounds was significantly improved. Research supported by: Moffitt Cancer Center

Abstract #: A-39 Presented by: Eva Christabel Williams, PhD, Graduate Student Smart Packaging: An Ingenious Technique For Targeted Drug Delivery For Ovarian Cancer Eva Christabel Williams,,Ryan Toomey, Norma Alcantar Department of Chemical & Biomedical Engineering,USF College of Engineering, Department of Chemical Engineering Keywords: niosome, ovarian cancer Objective: Ovarian cancer, commonly treated with chemotherapy, can produce overwhelming side effects in patients. To overcome the nonlocal character of chemotherapy, we have designed a smart packaged drug delivery system consisting of: non-ionic surfactant vesicles (niosomes) and a biodegradable temperature and pH sensitive hydrogel network. Methods: Fluorescent dye 5,6-Carboxyfluorescein was used to mimic the drug’s behavior. Reverse evaporation method was used to encapsulate the dye in the niosomes and were separated from free dye by ultracentrifugation or gel exclusion chromatography. Two different media were used (PBS and water) and monitored at 25° and 37°. Dynamic light scattering and transmission electron microscopy were used to study the niosomes size distribution. Results: It was found that the size distribution increased linearly with the dye concentration. It was observed that the release rate of dye was higher at 37?C and at larger concentrations of dye used during encapsulation. Additionally, it was observed that two factors decreased the release rate of dye: the presence of ions in solution (PBS), which provides stability to the niosomes; and the removal of free dye with ultracentrifugation, in which case niosomes dilution is prevented. Conclusion: The release rate as a measurement of stability and control of the drug delivery system depends on: pH, dye concentration, temperature and niosomes preparation protocol. The final outcome of this study includes the tuning of the variables above mentioned, that will contribute to the development of low cost and improved methods for drug delivery with application to intracavitary ovarian cancer treatment and other types of cancer. Research supported by: *FCoE-BITT Program *Signature Program in Neuroscience

Abstract #: A-40 Presented by: Nadine Bewry, BS, Faculty Bone Marrow Stromal Cells Activate Stat3 And Confer Resistance To Bcr-Abl Inhibitors in K562 Cells Nadine Bewry, Rajesh R. Nair, Moffitt Cancer Center and Research Institute, Mike F. Emmons, Moffitt Cancer Center and Research Institute, William S. Dalton, Moffitt Cancer Center and Research Institute, Lori A. Hazlehurst, University of South Florida and Moffitt Cancer Center and Research Institute University of South Florida College of Medicine Basic Biomedical Sciences - Molecular Medicine Keywords: Stat3, Bcr-Abl, CML, drug resistance Objective: Imatinib (IM) represents a potent molecularly targeted therapy against the oncogenic tyrosine kinase Bcr-Abl. Although IM has shown considerable efficacy against chronic myeloid leukemia (CML), it is not curative and overtime patients become refractory to treatment. However, the role of the tumor microenvironment on the emergence of IM resistance remains unknown. The predominant microenvironment of CML is the bone marrow, a rich source of soluble factors (SFs) which may influence drug response. Some of these SFs are capable of activating Stat3. Given Stat3’s crucial role in the survival of hematopoietic cells, our objectives are to determine, 1) does conditioned media (CM) derived from HS-5 human stromal cells activate Stat3 in K562 CML cells, and 2) does activation of Stat3 confer resistance to Bcr-Abl inhibitors. Methods: We utilized a transwell system to demonstrate that SFs secreted by the HS-5 stromal cells were sufficient to cause resistance to IM-induced apoptosis. Cell death was measured using AnnexinV apoptosis. SiRNA targeting Stat3 was used to determine its involvement in the drug resistant phenotype associated with CM. Results: CM protects K562 CML cells from apoptosis induced by IM and second generation Bcr-Abl inhibitors, nilotinib and dasatinib. K562 cells cultured in CM had increased phosphotyrosine-Stat3 levels. Reducing Stat3 levels using siRNA sensitized K562 cells to apoptosis induced by IM. Conclusion: SFs which activate Stat3 within the bone marrow microenvironment (BME) are sufficient to cause drug resistance. These data also highlight a novel mechanism of IM resistance and provide preclinical rationale for using Stat3 inhibitors to increase the efficacy of IM within the BME.

Abstract #: A-41 Presented by: M. Thure Caire, BS, Med II Student Increase in Survival Time of Patients Dying with AML at the H. Lee Moffitt Center M. Thure Caire, BS, John N. Greene, M.D., F.A.C.P. Chief, Infectious Diseases and Hospital Epidemiologist H. Lee Moffitt Cancer Center and Research Institute, Professor of Internal Medicine & Interdisciplinary Oncology, USF, COM, Albert L. Vincent, Ph.D. Division of Infectious Diseases and International Medicine Department of Internal Medicine, COM, USF, David Atrubin, Department of Health, USF, COM, Department of Internal Medicine Keywords: Infection, AML, Moffitt, Cancer Survival Objective: Infectious Diseases are a recognized cause of morbidity and mortality in Acute Myeloid Leukemia (AML) patients. This case series review attempted to quantify the contribution of infectious disease to mortality in AML patients at the H. Lee Moffitt Cancer Center, a National Cancer Network Center. Methods: Moffitt patients with AML who died between 2000 and 2006 (n=274) were aggregated by year of death. Trends in patient survival time were evaluated by linear regression. Contribution of infectious disease to mortality was examined by chart review of AML patients who passed in 2005 and 2006 with a record of death on file (n=32). Results: We observed a positive trend in mean patient survival time (r2=0.700, p=0.0197). In 2005 and 2006, infectious diseases contributed to the mortality of 75% (24/32) of cases reviewed. Conclusion: Infectious disease appeared to be a major contributor to the mortality of AML patients at Moffitt. Additionally, the survival time for AML patients was increasing significantly. This may be due to pharmacologic advancements and well as changes to treatment protocol for AML patients. Research supported by: University of South Florida College of Medicine Dean's Office Summer Research Program

Abstract #: A-42 Presented by: Toni Dobson, BS, Graduate Student Can We Identify Adroit Versus Maladroit Caregivers in the Adult Population of Cancer Patients with Pain? Toni Dobson, USF CON, Anthony Fulginiti, MSW, Moffitt Cancer Center Dr. S. McMillan, USF CON Dr. B. Small, USF CAS Keywords: caregiver pain depression quality of life Objective: To examine distinguishing characteristics between adroit and maladroit caregivers in the adult cancer patient population in order to identify and intervene in a timely and effective manner. Informal caregiving is a complex and difficult role for a family member or loved one to assume; this phenomenon receives inadequate attention by the healthcare delivery team. The maladroit caregiver needs to be recognized early enough for the health care system to intervene expeditiously and positively affect patient and caregiver outcomes. Evidence based, objective measures of this phenomenon must therefore be examined for clinical use in this vulnerable population. Methods: Approval for the abstract was obtained from the primary author. A literature search was conducted on the topic with virtually no results yielded in this population. Demographics and instruments were obtained from the RCT. The GIS, MCBS (C), CESD, and pain scores were compared with demographic information. Results: Please note: our SPSS calculations are being analyzed currently and will be available very soon. We are running frequencies to obtain medians, then recoding into the new variable 'the adroit caregiver', and running ANOVA on these, as well as post hoc testing. Conclusion: In progress. Will be available very soon.

Abstract #: A-43 Presented by: Christina Drenberg, BS, Graduate Student Semaphorin 3F Suppresses Telomerase Activity Christina Drenberg,Yira Bermudez, Sandra Livingston, Santo V. Nicosia, Patricia A. Kruk University of South Florida College of Medicine Department of Pathology and Cell Biology USF College of Medicine Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Telomerase, Semaphorin 3F, Ovarian cancer, VEGF Objective: Telomerase is a ribonucleoprotein that is active in more than 90% of human tumors, including ovarian cancers. Our lab has previously demonstrated that vascular endothelial growth factor (VEGF) stimulates telomerase activity in ovarian cancer through cellular VEGF receptors and subsequently via the ERK1/2 pathway targeting Sp1 sites within the hTERT promoter. Since semaphorin 3F (S3F), a proposed tumor suppressor, antagonizes VEGF the purpose of this study was to determine whether S3F inhibits telomerase. Methods: Established ovarian cancer cell lines ES-2, PA-I, SW626, and TOV112D were either treated with recombinant S3F protein or transiently transfected with S3F cDNA. Telomerase activity was measured by telomerase PCR-ELISA, while western immunoblotting was performed to detect S3F. Results: Western blot confirmed S3F transfection. We found that both transient transfection of S3F cDNA or treatment with S3F recombinant protein suppressed telomerase activity in ovarian cancer cell lines. Successful tranfection of NP-2 siRNA, targeting the S3F receptor, or treatment with the VEGF receptor inhibitor, CBO-P11, abrogated S3F mediated telomerase inhibition. Likewise, inhibition of the MAPK, ERK1/2, with U0126, diminished S3F mediated telomerase suppression. Conclusion: This data suggest that telomerase is a novel molecular target of S3F, which may be of clinical relevance. Ongoing research is focused on further delineating the molecular signaling pathway/mechanism(s) by which S3F regulates telomerase.

Abstract #: A-44 Presented by: Maria Khambaty, BS, Med II Student Cervical Cancer Screening Among HIV-Infected Women at the Local Health Department Maria Khambaty, BS, Jennifer L. Logan, MD (Faculty, COM, Department of Internal Medicine), Lynette J. Menezes, PhD (Faculty, COM, Department of Internal Medicine), William A. Lapcevic, MSST ,COPH, Department of Epidemiology and Biostatistics, Tao Wang, PhD (Faculty, COPH, Department of Epidemiology and Biostatistics, USF, COM, Department of Internal Medicine Keywords: cervical cancer, HPV, pap smear, HIV Objective: This study determined the proportion of HIV-infected women who were screened for cervical cancer as per the Center for Disease Control and Prevention’s (CDC) guidelines. The study also examined associations between demographic factors and receipt of CDC-recommended cervical cancer screening. Methods: Medical records of 200 HIV-infected female subjects were randomly selected from patients who established care at the Hillsborough County Health Department between January 1, 2000 and May 1, 2006. Data related to cervical cancer screening and demographics were extracted from these records and analyzed using Statistical Analysis Software (SAS). Results: The sample included 60.1% Blacks and 39.38% Whites. 21.88% of subjects reported Hispanic ethnicity. Most women (86.98%) reported heterosexual contact as the primary HIV risk-behavior and 10.42% reported injection drug use. Subjects’ mean reported income was $8180. At enrolment, 52.11% were unfunded and 47.37% received public assistance for healthcare. Overall, 83% had received at least one pap smear in their first year after enrolment; 24.5% of women received the recommended two pap smears; and 17% received zero pap smears. Conclusion: For HIV-infected women, cervical cancer is aggressive but preventable with appropriate screening. Further research is needed to address the reasons for the low prevalence of recommended cervical cancer screening among these women. Appropriate strategies must be introduced at the health department to ensure that HIV-infected women are screened regularly to prevent cervical cancer.

Abstract #: A-45 Presented by: Cecile A. Lengacher, PhD, Faculty Mindfulness-Based Stress Reduction: Research Synthesis and State of the Science Cecile A. Lengacher, PhD,Stacey Boutilier MS, RN, USF CON, Harleah Buck MS, RN, USF CON, Melissa Molinari RN, USF CON Keywords: MBSR, meditation, cancer, stress Objective: To describe the state of the science of MBSR through a research synthesis of published research on the use of MBSR among patients with cancer and other health conditions. Methods: Multiple databases, including OVID, PsychINFO, and PubMed, were utilized to review research from 1982-2007. MBSR and meditation were key search terms. Inclusion criteria: 1) Research studies published prior to February 2007. Empirical studies, RCTs, descriptive, or quasi-experimental research, and case studies. 2) Use of the MBSR intervention according to standardized training format (meditation program, daily practice, group format, quantitative outcomes measures, length 6-12 weeks, and 1.5-3 hour weekly sessions). 3) Research published in English. Exclusion criteria: 1) Anecdotal notes. 2) Studies in other languages. 3) Abstract only articles. 4) Dissertations. Results: Two hundred eighty-one studies were identified with the criteria and 47 were reviewed. Beginning of the Science (1982-1995): 8 studies were published with pre-test, post-test, quasi experimental designs with no control groups. Advancing the Science (1997-2007): 16 randomized studies (4 among cancer patients and 12 non-cancer); 24 non-randomized studies (7 among cancer patients and 17 non-cancer). Conclusion: Although studies report the effectiveness of MBSR in reducing pain, depression, anxiety, affecting mood, and increasing quality of life, their generalizability to patient populations is questionable due to few randomized research designs. Moreover, preliminary evidence indicates patient improvements were sustained for up to 4 years. Limitations of the studies include poor research designs, variability among instruments used to assess similar outcomes, and variability of delivery time of the intervention.

Abstract #: A-46 Presented by: Qiuping Ma, MS, Graduate Student Repression of AR N/C Interaction by FoxO1 and Its Involvement in the Suppression of the AR Activity by PTEN Tumor Suppressor Qiuping Ma, Wei Fu1, Pengfei Li1, Santo V. Nicosia1,2,4, Guido Jenster5, Xiaohong Zhang1,2,3, and Wenlong Bai1,2,3 Departments of Pathology and Cell Biology1 and Interdisciplinary Oncology2, USF COM and Programs of Molecular Oncology3 and Experimental Therapeutics4, H. Lee Moffitt Cancer Center, 5Department of Urology, Josephine Nefkens Institute, Erasmus MC. USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: androgen receptor, prostate cancer, FoxO Objective: PTEN tumor suppressor suppress the Androgen Receptor activity through its downstream FoxO1. Methods: Cell culture and transfection; Adenovirus Infection; Immunoblotting Analysis; Colorimetric MTT Assay; SiRNA techniques Results: we report the repression of the activity of full-length AR and its N-terminal domain (NTD) by FoxO1 and the involvement of FoxO1 in the inhibition of the AR by PTEN. Overexpression of FoxO1 suppressed AR activity in PC3 and LNCaP cells as well as androgen-induced cell proliferation and production of prostate-specific antigen in LNCaP cells. Knocking down of FOXO1 by RNA interference increased the transcriptional activity of the AR in RWPE-1 cells and relieved its inhibition by PTEN in PC3 cells. Active AKT increased AR activity in RWPE1 cells and the co-expression of a constitutively nuclear FOXO1 relieved the increase. Mutational analysis revealed that FoxO1 (150-655) including the Forkhead box and C-terminal activation domain is required for the transcriptional inhibition. Mammalian two-hybrid assay demonstrated that the inhibition of AR activity by PTEN and FoxO1 involved the interference of the androgen-dependent N-terminal and carboxyl-terminal (N/C) interaction and the recruitment of p160/SRCs to AR NTD by FoxO1. Conclusion: In summary, our studies identify new mechanisms for FoxO1-mediated AR suppression and establish FoxO1 as an important nuclear factor that mediates the mutual antagonism between the AR and the PTEN tumor suppressor in prostate cancer cells.

Abstract #: A-47 Presented by: Vevek Parikh, Med II Assay for the IFN-gamma Signaling Pathway Component, Phospho-STAT1, in Tumor Microarrays. Vevek Parikh(1), Domenico Coppola(2), and George Blanck(1). (1) Department of Molecular Medicine, USF COM; (2) Moffitt Cancer Center and Research Institute Objective: To determine whether tumors could be stratified on the basis of the presence of pro-apoptotic factor, phospho-STAT1. Methods: About 200-260 samples were placed on each microscopic slide, thus creating a tissue microarray (TMA). Three tumor types, breast, pancreas, and colon, were stained for phospho-STAT1. Phospho-STAT1 staining was considered positive where there was significant nuclear staining of two or more nuclei. Results were then tabulated to obtain the percentage of each tumor type that stained positive. Results: Our findings indicate that there were substantial differences between the level of phospho-STAT1 in the tumor versus the non-tumor tissues for breast and colon cancer, but not for pancreatic cancer. In addition, we observed tumor heterogeneity with respect to nuclear phospho-STAT1. Conclusion: These findings indicate that tumors may be stratified on the basis of phospho-STAT1 presence, leading to questions such as, do patients with phospho-STAT1 positive tumors have a better prognosis or are they better suited for certain therapies?

Abstract #: A-48 Presented by: David Sallman, BS, Med II Student Clusterin Mediates TRAIL Resistance In Prostate Tumor Cells David A. Sallman, Xianghong Chen, Bin Zhong, Danielle Gilvary, Junmin Zhou, Sheng Wei, and Julie Y. Djeu Affiliation of all Immunology Program, H. Lee Moffitt Cancer Center and Department of Interdisciplinary Oncology, USF, COM, Tampa, FL USFCOM, Interdisciplinary Oncology Keywords: Stat1, clusterin, TRAIL, resveratrol, prostate cancer Objective: One of the major obstacles in curing advanced prostate cancer is the development of drug resistance to docetaxel, which is the gold standard for the treatment of this disease. It is not only imperative to discover the molecular basis of resistance, but also to find therapeutic agents that can disrupt the resistant pathways. Methods: Docetaxel resistant prostate tumor cells were utilized for all experiments. Flow cytometric analysis was performed to determine the amount of apoptosis induction upon treatment with resveratrol, TRAIL, or a combination of the above. The tumor cells were treated with a siRNA targeted for clusterin expression and then treated with TRAIL alone and apoptosis was measured using the Annexin V assay. Also, western blot analysis was performed following resveratrol treatment to analyze the expression of clusterin, Stat1, Src, and Jak. Results: The combination of TRAIL and resveratrol synergistically induced apoptosis in our docetaxel resistant cell lines. From the siRNA experiment, TRAIL resistance in our cells was found to be dependent upon the expression of clusterin. Resveratrol functions as a tyrosine kinase inhibitor by inhibiting the Jak/Src-Stat1 pathway which we have previously shown to regulate the expression of clusterin. Conclusion: From this study, we have discovered a novel finding that resveratrol can mediate anti-tumor properties through inhibition of specific tyrosine kinase pathways. TRAIL resistance in docetaxel resistant prostate cancer is mediated through clusterin expression and that resveratrol can sensitize these cells through inhibition of the Src/Jak-Stat1 pathway. Adding these agents in combination with docetaxel could prove to increase the survival of patients with advanced prostate cancer.

Abstract #: A-49 Presented by: Aisha Shaheed, BS, Graduate Student A Novel Biochemical Treatment: Monoclonal Antibodies Aisha Shaheed, BS,,Sateesh R. Prakash, MD Aliyah Baluch, MD USF, College of Arts and Sciences, Department of Chemistry Keywords: monoclonal antibodies, cancer, Rituximab, Trastuzumab Objective: Monoclonal antibodies (MAbs) are antibodies of a single specificity. The emerging use of MAbs as therapies is one of the most significant advancements in medicine. The goal of our study was to evaluate the overall effectiveness and continual usage of MAbs as therapeutic agents in the various fields of medicine. Methods: A literary analysis reviewing 8 years of studies using MAbs in terms of their overall effectiveness for cancer, autoimmune diseases, diabetes mellitus and cardiovascular disease (CVD) treatment. Results: In 2003 Rituximab, an anti-CD20 MAb targeting B-cell non-Hodgkin lymphoma, demonstrated in 166 patients a reduction in tumor size greater than 20% in 76% of the patients. Standard chemotherapy combined with Rituximab also gave a significant increase in life expectancy. Trastuzumab, another MAb, was developed to treat HER2neu positive breast cancers. The tumor regression rate for the combined treatment group compared to chemotherapy alone was 59% vs. 17% respectively. Trastuzumab is being considered as a treatment for other cancers as well, i.e. gastric cancer. The next step was to evaluate options for MAb use in other diseases. Trials with Infliximab have been done with Crohn’s disease and rheumatoid arthritis with significantly positive results. Paradigms for treatment of diabetes mellitus and CVD are now evaluating the use of MAbs as well. Conclusion: MAbs have proven to be very beneficial over recent years. MAbs serve as the gateway to a promising future for healthcare. MAbs should continue to offer promising treatments for diseases once considered untreatable.

Abstract #: A-50 Presented by: Brian Boone, BS, Med III Student Gene Therapy in the Heart Utilizing Synchronous Electroporation Brian Boone, BS, USF,COM, William G. Marshall, Jr., MD, MBA, Dept of Surgery, USF COM Sylvia Gografe, PhD, DVM, Vanderbilt Univ., Margaret Baldwin, MS, Dept of Comparative Medicine, USF Michele Danielson, BA, Dept of Comparative Medicine, USF Jane Larson, BS, Dept of Comparative Medicine, USF Dennise Caretto, CVT, Dept of Comparative Medicine, USF Yolmari Cruz, BS, Bernadette Ferraro, BS, Dept of Molecular Medicine, USF COM, Mina Guirguis, USF, Deli Zamani, USF, Ani Chawdry, USF, Megan Mabis, USF, Shamik Dwivedi USF, Kenneth Ugen, PhD, Dept of Molecular Medicine, USF COM Mark Jaroszeki, PhD, Dept of Electrical Engineering, USF COE Richard Heller, PhD, Dept of Molecular Medicine, USF COM ,Dept of Surgery Keywords: Gene therapy, Electroporation, VEGF Objective: Electroporation has been demonstrated to be an effective method of gene delivery in the in vivo heart. However, application of asynchronous electrical pulses caused ventricular fibrillation. This study evaluated delivery of plasmids encoding genes for luciferase or vascular endothelial growth factor (VEGF) to the in vivo heart using electrical pulses synchronized to the cardiac electrical cycle to prevent ventricular fibrillation. Methods: Eight farm pigs (neutered males, 25 – 30 kg) underwent median sternotomy under general endotracheal anesthesia. Plasmids coding for luciferase or VEGF were injected in the left ventricular wall followed by delivery of synchronous electroporation at various parameters. Utilizing a surface electrocardiogram, electrical pulses were applied to the heart synchronously with the QRS complex. One site of plasmid delivery without electroporation was placed following completion of all electroporation. Animals were maintained for 2 or 7 days and injection site excision completed with a 6 mm punch. Levels of luciferase and VEGF expression were quantified from the tissue samples. Results: No pig fibrillated during the procedure. Myocardial expression of luciferase was increased 1000X (p<0.05 vs control) with synchronous electroporation. VEGF expression was greatly enhanced (5 – 8X) versus plasmid alone when delivered with synchronous electroporation (p<0.1). Conclusion: The use of synchronous electroporation as a method of gene transfer into the in vivo heart was successful. Synchronous electroporation pulses were safe and effective; prevented ventricular fibrillation and achieved increased levels of luciferase and VEGF gene expression. Research supported by: University of South Florida College of Medicine Feasibility Grant

Abstract #: A-51 Presenter: Shaili Gal, MS II Cytokine Profile after Transplantation of Human Umbilical Cord Blood post-Myocardial Infarction Shaili Gal, BS,, Brian Boone, BS, USF, COM; Sylvia Gografe, PhD, DVM, Vanderbilt Univ; Margaret Baldwin, MS, Michele Danielson, BA, Jane Lawson, BS, Dennise Caretto, CVT, Dept of Comparative Medicine, USF COM Deli Zamani, USF Jose’ Burgos, MD, , William G. Marshall, Jr., MD, MBA, Dept of Surgery, USF COM Key Words: cytokines, stem cells, umbilical cord blood, myocardial infarction Objectives: This study evaluated the time course of serum cytokine levels (TNF-a, IL-1B, IL-6, IL-8, and IL-10) following transplantation of human umbilical cord cells (hUCBC) after acute myocardial infarction (AMI) in a porcine model. Methods: 17 adult Yucatan pigs (neutered males, weights 25 – 35 kg.) underwent medial sternotomy under general endotracheal anesthesia. The left anterior descending artery was occluded for 30 minutes. After 2 minutes of re-perfusion, 1 of 3 therapies was administered: I – MI only, II – Isolyte, III – hUCBC. Immunosuppresssion (cyclosporine) was administered to one half of each group during the protocol. Serum cardiac enzyme (CK-MB, Troponin) and cytokine samples were drawn following anesthetic induction, and 1, 6, 12, 24, and 48 hours post – infarction. Animals were sacrificed at 8 weeks. The heart was examined for presence of trans-differentiated cells or presence of human stem cells by immunohistochemistry. Results from Groups I and II were combined for analysis vs. Group III. Results: All animals sustained myocardial necrosis determined by Troponin and CK-MB. Pathology revealed no human stem cells or trans-differentiated cells present by immunohistology in any specimen. Group III without immunosuppression had higher levels of TNF-a, IL-10, and IL-1B than Group I+II. There was no difference in the levels of IL-6 and IL-8. Group III with immunosuppression, had increased levels of IL-8, IL-10, and IL-1B versus Group I+II. There was no difference in IL-6 and TNF-a. Conclusions: Transplantation of hUCBC in AMI affects cytokine levels post-infarct. Further study is necessary to assess the anatomic and physiologic effects on the heart. Research Supported by: STTR Phase 1 Grant: Cord Blood: Treatment for Acute Myocardial Infarction (NIH/NHLBI # 1 R41 HL077012-01 A1) Florida Hi-Tech Corridor Grant: Cord Blood: Treatment for Acute Myocardial Infarction

Abstract #: A-52 Presented by: Adam Guyer, MS, Med I Student Human Umbilical Cord Blood Cell Treatment of Ischemic White Matter Adam Guyer, MS, COM, Aaron Hall (MPP), Chris Leonardo (MPP), Craig Ajmo (MPP), Lisa Collier (MPP), Keith Pennypacker (MPP), Alison Willing (Center for Aging in Brain Repair, MPP) USF, COM, Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Myelin Basic Protein; Ischemia; stem cells; white matter injury; oligodendrocytes Objective: Previous reports have shown human umbilical cord blood cells (HUCBCs) administered intravenously 48-hrs following middle cerebral artery occlusion reduces the infarct area and behavioral deficits of rodents. This cellular therapy is potently neuroprotective and anti-inflammatory. This study investigates the role of HUCBC treatment on white matter injury and oligodendrocyte survival in rodent models of hypoxia-ischemia. Methods: Male Sprague-Dawley rats underwent middle cerebral artery occlusion (MCAO) surgeries and given intravenous infusions of HUCBCs 48 hrs post stroke. The rodents were sacrificed 96 hrs following stroke; the brains were sectioned and stained with myelin basic protein (MBP) to examine white matter damage. To determine whether HUCBC treatment was protective via direct effects on oligodendrocytes, cultured oligodendrocytes were studied in an in vitro model of oxygen glucose deprivation (OGD). Effects of OGD on oligodendrocytes co-cultured with HUCBCs was assessed using caspase 3 immmunocytochemistry, as well as LDH assay for cytotoxicity. Results: Intravenous infusion of 10^6 HUCBCs 48-hrs post-stroke reduced the amount of white matter damage in vivo as seen by quantification of MBP staining in tissue sections. Cleaved caspase 3 immunohistochemistry and the LDH assay for cytotoxicity showed that HUCBCs provide protection to oligodendrocytes in vitro. Conclusion: Based on these results it is likely that HUCBC administration directly protects oligodendrocytes and white matter. This effect is likely to contribute to the increased behavioral recovery observed with HUCBC therapy.

Abstract #: A-53 Presented by: Sarah Norring, MS, Graduate Student Glycosylation Impacts Gating of the hERG1 Channel Sarah Norring, MS,Tara A Schwetz, Eric S Bennett USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: hERG, glycosylation, Kv channel Objective: Arrhythmias are often caused by aberrant ion channel activity, resulting in remodeling of the cardiac action potential. Two K+ currents, IKs and IKr, contribute to phase III repolarization of the human action potential. Human ether-a-go-go-related gene 1 (hERG1), a voltage-gated potassium channel, underlies IKr. Alterations in the repolarization phase of the action potential, and in particular IKr, can lead to arrhythmias, long QT syndrome, and sudden cardiac death. Our lab previously determined that sialic acids, often the terminal residue of N- and O-linked carbohydrate structures, can alter gating of voltage-gated Na+ channels and the Drosophila Shaker K+ channel. hERG1 has two potential N-glycosylation sites located in the S5-S6 linker region. The aim of this study was to determine if and to what extent glycosylation modifies hERG1 channel function. Methods: Using whole cell recordings, the voltage-dependent gating and kinetics of hERG1 were evaluated under conditions of full glycosylation, no sialylation, and in the absence of complex glycans. Results: Under conditions of reduced sialylation and also in the absence of complex glycans, the half-activation voltage (Va) for hERG1 shifted to more depolarized potentials by ~10 mV. Reduced sialylation and complex glycans impacted steady-state inactivation. The half-inactivation voltage (Vi) for hERG1 shifted to more depolarized potentials by ~13 and ~19 mV, respectively. Additionally, a reduction in the levels of sialic acids and in complex glycan caused a significant 38% and 76% increase in deactivation rates at -50 mV. Conclusion: These data indicate a functional role for glycosylation in the modulation of gating of the hERG1 channel, suggesting that N-linked sugars promote hERG1 activity. Research supported by: AHA

Abstract #: A-54 Presented by: Tara Schwetz, MS, Graduate Student N-Linked Sialic Acids Are Responsible For The Entire Effect Of Sugars On Kv1.5 Gating, While Other Sialic Acids Modulate Kv2.1 Gating Tara Schwetz,,Sarah A. Norring, Eric S. Bennett USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Kv1.5, Kv2.1, sialic acid, N-glycosylation Objective: Kv1.5 and Kv2.1 produce a slowly inactivating current responsible for a portion of cardiac repolarization. Kv channels are heavily and differentially glycosylated, with glycosylation sites varying in number, location, and type of sugar linkages. Previously, our lab showed that loss of the terminal sugar residue, sialic acid, modulates gating of the Drosophila Shaker K+ channel, resulting in a depolarizing shift in the voltage of half-activation (Va). Here, we investigated whether alterations in glycosylation, including sialic acids, can modulate voltage-gated potassium channel function. Methods: Using whole cell recording methods, voltage-dependent gating of Kv1.5 and Kv2.1 was evaluated under conditions of full and effectively no sialylation. Results: Under states of reduced sialylation, the Va for Kv1.5 and Kv2.1 were shifted significantly to more depolarized potentials by 21 mV and 8 mV, respectively. The asparagine residues of the N-glycosylation consensus site for Kv1.5, located on the S1-S2 linker, and Kv2.1, located on the S3-S4 linker, were mutated to prevent the addition of N-glycosylation. For Kv1.5, the data suggest that N-linked sialic acids account for the entire effect of sialic acids on channel gating. However, Kv2.1 mutagenesis had no effect on the impact of sialic acids on channel gating, indicating that, if Kv2.1 is N-glycosylated, these sugars do not exert any measured functional effect. These data suggest that sialic acids other than N-linked sialic acids modulate Kv2.1 gating. Furthermore, similar sialic acid-dependent shifts were observed for the Kv1.5 and Kv2.1 activation time constants and Va. Conclusion: Overall, the data indicate a functional and isoform-specific role for sialic acids in the modulation of Kv channel gating. Research supported by: AHA

Abstract #: A-55 Presented by: Manoj Agravat, MPH, Graduate Student Method For Calculating Odds Ratios Relative Risks, And Interaction Manoj Agravat, MPH, USF COPH, Department of Epidemiology & Biostatistics Keywords: CMH test confounding effect modification Objectives: Introduce a method that calculates odds ratios and relative risks testing for confounding and effect modification that is comparable to CMH test and B-Day test.

Methods: R program using survreg: countrya<-survreg(Surv(count,smokers)~country, data=country1, dist=”Rayleigh”);βz (country)= .379; SEz=.458; for ~country:smokers βxz=-.0416; βy=-1.04 & SEy=.289; Controlling for z: OR1=(exp^(βz)/exp^(-(βz+βxz)xsqrtSEz=(1.46)/(.7136)xsqrt.458=.1.385; CMHtest= 1.39;RR1=exp(-(βz+βxz))/(1-exp^(βz+βxz))xsqrtSEz=(.7136)/(.401)xsqrt.458=1.20; CMHstat=1.28; RR2=exp^(βy+βxz) / (exp^(βy))=.339/.353=.96; CMHstat=.92; Controlling for x: OR2=exp^(-(βz))/(1-exp^(βz)xsqrtSEz=(.6845)(>4608)xsqrt.458=1.00; no CMHstat; RR1=exp^(-(βy))/(1-exp^((βy) xsqrtSEy =.(2.82)/(.6465)xsqrt..289=2.34; no CMHstat;RR2=(exp^(βy+βxz)exp^(1-(βy))xsqrtSEz =(.2706)/(.6465) = .4186;( no CMHstat); SAS program for p: (note: here use absolute value of β’s; input cases exp z x n @@; datalines; 1 1 1 1 73 0 1 1 1 188 1 0 1332 79 21 0 0 5201 307 82…; 73 and 188 raw counts; new estimate where y=1 and raw count(21)/ βzxβxz; where y=0, new est.= raw count/βyz; proc logistic data=passlung descending; class exp (ref=’1’); weight n; model cases=fit z x/lackfit;

Results: At α=.05,smoker is significant with p=.019 . With country and smokers in model, p=.043. New p value=.611 for test of effect modification.

Conculsion: One suspects confounding by country based on p values. Statistic for effect modification, p= .611 other than B-Day test (p=.88), One concludes no effect modification.

Abstract #: A-56 Presented by: George Barbier, MD, Faculty The PACS Challenge George Barbier, MD, Anne Curtis, M.D. USF CV Crisp Lynn Martinez, Lead Clinical Applications Coordinator, Tampa VA Kirsten Wilson, Administator Clinical Procedures, VA Tampa Gwen Pinder, RN Informatics, VA Tampa Al Tuting, Systems Adminstrator, VA Tampa, USF COM Department of Internal Medicine Keywords: informatics, PACS, picture archiving and communication system, DICOM, digital imaging and communication in medicine, hospital information system, electronic medical record Objective: Evaluate the effectiveness of a totally digital cardiology picture archiving and communication system in quality performance as measured by the American College of Cardiology-National Cardiovascular Data Base Registry(ACC-NCDR) Methods: Using a cedaron software package, quality performance data on percutaneous coronary intervention was extracted from the first VA totally integrated and functional cardiology PACS, picture archiving and communication system for the ACC-NCDR cath lab database. Results: Between 2005 and 2007, our performance measures reported to the ACC NCDR improved and compared favorably with participating national medical center databases. Conclusion: All measured paramaters continuously improved at the only VA Medical Center in the nation to participate in the ACC-NCDR registry. Notable was the risk adjusted mortality and use of thienopyradines on discharge in which Tampa VA ranked above the 95%.

Abstract #: A-57 Presented by: Boubakari Ibrahimou, PhD, Graduate Student Modeling of Criteria Air Pollutants Data: a First Step to Linking Air Pollution to Childhood Asthma Attack Boubakari Ibrahimou, PhD, Yiliang Zhu, PhD. Dept. of Epidemiology & Biostatistics COPH USF Keywords: Air Pollutant, Asthma, modeling Objective: Time series modeling of criteria Air Pollutants is one of the steps in addressing “temporal and spatial congruence of existing environmental monitoring, personal exposure measures, and health outcome” and explores the effects of spatiotemporal elevations in four air pollutants – ground level ozone (O3), particulate matter (PM), sulfate dioxide (SO2), and nitrogen dioxide (NO2) - on asthma attack in children. A specific aim is to quantify the elevations and the duration in which they occurred, from the residuals of these models, and subsequently assess the relationships between elevated exposure to the air pollutants and asthma. Methods: Data collected from the national monitoring network of the Environmental Protection Agency are used, and time series analyses were conducted using such models as autoregressive, moving average and integrated moving average. Results: A mathematical model of the form X(t)= P(t) + a Cos(wt)+b Sin(wt)+ ARIMA(p, d, q) + error is fit to the data to capture both seasonality and trend observed in the data. Illustration is given using ozone data in Los Angeles area. Conclusion: The model proposed is fully capable of describing the seasonal variation and long term trend in air pollution. High levels of elevation are noticeable from the fitted model. A second stage of screening will help identify periods of high level elevations in pollutants in conjunction with serial data of childhood asthma.

Abstract #: A-58 Presented by: Teri Malo, MPH, Graduate Student Using Structural Equation Modeling to Test the Theory of Reasoned Action: Predicting Sexual Behavior Teri Malo, MPH, USF COPH< Department of Community & Family Health Keywords: adolescents, sexual behavior, Theory of Reasoned Action Objective: This study examined proximal, intrapersonal factors associated with delaying first sexual intercourse in a national sample of adolescents. Specifically, this research investigated the utility of the Theory of Reasoned Action (TRA) as a plausible model for predicting whether an adolescent engages in sexual intercourse. Methods: This analysis used data from Waves I and II of the National Longitudinal Study of Adolescent Health (Add Health) to test the applicability of the TRA (n = 3,395). Structural equation modeling tested the TRA constructs simultaneously and accounted for measurement error. Results: With the exception of the path from intention to behavior at Wave II, the standardized regression weight and all probit regression coefficients were statistically significant (p<.05): path between attitude and subjective norm = -.301, from attitude to intention = .328, subjective norm to intention = -.415, intention to behavior at Wave I = -.557, and behavior at Wave I to behavior at Wave II = .854. Although not part of the proposed TRA model, the modification indices suggested the addition of a path from subjective norm to behavior at Wave I (.186). Conclusion: The TRA may be useful for predicting whether an adolescent engages in sexual intercourse. All measured variables for subjective norm concerned one’s perception of parental approval or disapproval of engaging in sexual intercourse. Researchers should continue to explore the importance of perceived norms, particularly the influence of parental perceptions. Educators and other practitioners should continuously reevaluate theoretical models on which programs and interventions are based, and structural equation modeling is a useful statistical technique for examining the practical utility of theory.

Abstract #: A-59 Presented by: Yicheng Tu, PhD, Faculty Database-Centric Analysis of Molecular Simulation Yicheng Tu, PhD,,Sagar Pandit, Department of Physics, College of Arts and Science Ivan Dyedov, Department of Computer Science and Engineering, USF College of Engineering, Department of Computer Science and Engineering Keywords: Molecular simulation, data analysis, database, query processing, indexing Objective: Studies on molecular dynamics has attracted a great deal of attention from the research communities of biology, physics, and chemistry. Computer simulation is an important method for molecular dynamics research. The outcome of an MD simulation is a "pseudo trajectory" of the positions, velocities, and many other physical measurements of all of the atoms in the simulation system. Due to the large number of atoms/molecules and time instances to simulate, such simulations generate tremendous amounts of data. The main objective of this project is to develop a framework for effective and efficient sharing and analysis of MS data. Methods: Current strategies to store, access, and query such data are all based on computer files. This renders some serious efficiency problems in data sharing, application development and runtime query processing. We propose the idea of using database management systems for data storage and query processing. We also propose novel indexing methods and new query processing algorithms to achieve efficient data processing. Results: Our experimental results based on a prototype of the proposed data processing framework further show that 1) the duration of application development cycle is greatly reduced as most queries in our framework involved a few lines of code (vs. hundreds of lines of code in current systems); 2) response times to queries are 1 to 2 orders of magnitude lower than in the file-based solution. Conclusion: Database technologies can be utilized to revolutionize MS data processing and analysis by significantly increasing the efficiency in application development and query processing.

Abstract #: A-60 Presented by: Monika Wahi, MPH, Graduate Student Changing the Reference Group in Published Odds Ratios for Multi-Level Categorized Exposures Monika Wahi, MPH,,Skai W. Schwartz PhD Department of Epidemiology and Biostatistics, USF COPH Keywords: Statistics, meta-analysis, odds ratio, methods, epidemiology Objective: Challenges to conducting a meta-analysis include combining information (1) from analyses with variable levels of adjustment; (2) using incomparable populations across studies; (3) using different categorizations of a continuous variable and/or (4) different reference groups. This paper explores standardizing the reference group across multiple studies. Methods: We demonstrate various approaches using an example dataset. Results: For two levels of exposure, this is a straightforward process. For example, if the measure of association is an odds ratio (OR), one simply takes the reciprocal of the OR and the lower and upper bounds of the confidence interval. However, with a multi-level exposure, obtaining new confidence intervals is hampered by lack of a covariance estimate. Conclusion: Care should be taken to report ORs using reference groups consistent with those used in previous articles. Alternatively, an option to print the variance-covariance matrix is usually available in the modeling procedures of commonly used statistical software packages. Some journals may provide guidelines requesting the variance-covariance matrix to be in an appendix.

Abstract #: A-61 Presented by: Mariana Arevalo, BA, Graduate Student Assessing the Validity and Reliability of a Spanish Version of the City Stress Inventory Mariana Arevalo, Kristen J. Wells, PhD, MPH. USF COPH, Department of Community & Family Health Keywords: CSI, validity, reliability, hispanics, culturally appropiate measures. Objective: The purpose of this study was to evaluate a translated version of the City Stress Inventory (CSI; Ewart & Suchday, 2002) for reliability and validity. Methods: Internal consistency was evaluated by Cronbach’s alpha and examining item-to-total correlations. Then, rural participants were compared to urban participants on exposure to violence and neighborhood disorder subscale scores using an independent samples t-test. Finally, the associations between the subscales of the CSI and measures of depression and stress were evaluated by Pearson correlations. Results: Neighborhood Disorder and Exposure to Violence subscales had good internal consistency in most items. Neighborhood Disorder subscale was significantly positively correlated with Exposure to Violence and Stress and Neighborhood Disorder subscale was positively correlated with Neighborhood Disorder and Stress. Neither subscale was significantly correlated with depression. Conclusion: Both CSI subscales demonstrated good internal consistency with two items removed from each subscale. They also showed concurrent validity in the high positive correlation with another stress measure.

Abstract #: A-62 Presented by: Kristy A Siegel, MPH, Graduate Student Communicating the Sciences and Risks of Microconstituents in Reclaimed Water: Meta-Analytical Study Kristy A Siegel, MPH Dr. Foday Jaward, COPH USF, Department of Environmental & Occupational Health, Dr. Jeffrey Cunningham and Dr. Daniel Yeh, COE USF, and Dr. Derina Holtzhausen, CAS USF Keywords: Reclaimed water, microconstituents, communication Objective: As the water shortage crisis worldwide worsens, alternative water supplies, such as reclaimed water, are being sought. The aim of this study was to provide the background for a project that will examine the multidisciplinary aspects of communicating the science and risks of emerging microconstituents in reclaimed water. Methods: Due to the multidisciplinary nature of the larger project, a search of various electronic databases was conducted. Keywords were used as single terms, phrases, and complex Boolean search strings. Inclusion criteria were: peer-reviewed papers published in English (1990 to present); details of methodology of study techniques; and primary research. The references of all articles were screened as well. Results: The abstracts of 54 articles published in peer-reviewed science or engineering journals were examined. Of these, 38 articles were accessible for hardcopy review. Conclusion: Before any reclaimed water project can become a reality, the public must accept it. Health consequences were the primary concern when examining public attitudes towards various reclaimed water uses. As such, research is needed to demonstrate the safety of reclaimed water. However the definitive data on reclaimed water which would support no adverse health effects is lacking. Moreover, no requirement currently exists to conduct analyses for unregulated organic contaminants potentially present in wastewater at microgram-per-liter levels. Likewise, the human heath effects of the emerging microconstituents are unknown. Research supported by: USF Graduate School, part of the Graduate Multidisciplinary Scholar program

Abstract #: A-63 Presented by: Michelle Zeager, MPH, Graduate Student Nitrogen Dioxide Measurements in Neighborhoods in Hillsborough County Michelle Zeager, MPH, Amy Stuart, Ph.D., Assistant Professor, USF COPH, Department of Environmental & Occupational Health Keywords: Nitrogen Dioxide, spatial variation, air pollution, passive sampling Objective: Nitrogen dioxide has been linked to airway inflammation, asthma exacerbation, decreased lung function, and increased susceptibility to infection, particularly in vulnerable populations such as children and those with existing lung and cardiac disease. It has also been linked to increased cardiac morbidity. The objectives of this study are to determine NO2 levels in neighborhoods throughout Hillsborough County, to determine the impact of proximity to major highways on NO2 levels, and to determine if minority school children are exposed disproportionately. Methods: NO2 levels will be measured using Ogawa passive samplers deployed for 24 hours in neighborhoods near approximately 75 randomly chosen elementary schools in Hillsborough County. Several duplicate samplers will be placed as well as blanks for quality control. The samples will then be extracted and analyzed using colorimetry and visible spectrophotometry. The data will be compared to neighborhood and school demographic information, roadway proximity data, and traffic count information. Results: As fuel combustion is a significant source of NO2, we expect to find increased concentrations at locations that are in close proximity to major roadways with higher traffic counts. Based on previous work, we expect to find higher concentrations in neighborhoods and near schools with higher minority populations. Conclusion: None yet. Research supported by: USF COPH, Department of Environmental and Occupational Health

Abstract #: A-64 Presented by: Kent Alipour, Undergraduate Voluntary Ethanol Consumption is Greater in Adulthood After Adolescent Binge Ethanol Exposure. Kent Alipour, Antoniette M. Maldonado, M.A. & Cheryl L. Kirstein, Ph.D. Cognitive and Neural Sciences, Department of Psychology, USF CAS, Department of Psychology Keywords: Binge, Alcohol, Adolescence, Repeated Administration Objective: Binge alcohol consumption is a rising concern in the United States, especially among adolescents. During this developmental period binge alcohol use is usually initiated. A binge model has been established in animals. Animals are repeatedly administered high doses of ethanol over four days. While previous work has examined the effects of a single four-day binge model, there is sparse work done examining the behavioral consequences of repeated four-day binges. The repeated four-day binge model may better model human adolescent patterns of alcohol use. Methods: The present experiment examined the effects of repeated binge ethanol (EtOH) during adolescence on voluntary ethanol intake in adulthood in male rats. On postnatal day (PND) 28-31, PND 35-38 and PND 42-45, EtOH (1.5, 3.0 or 5.0 g/kg) or water was administered to adolescent rats. All rats then underwent abstinence from PND 46-59. Subsequently, in adulthood (PND 60-69), rats were assessed for voluntary EtOH intake using a limited access two-bottle choice paradigm. Results: Repeated binge EtOH during adolescence, regardless of dose, enhanced voluntary EtOH intake in adulthood relative to controls. Moreover, rats showed a dose-dependent increase in voluntary EtOH intake across days in adulthood. Specifically, rats that were exposed to higher doses showed a faster rate of increased in EtOH consumption. Conclusion: Together, these data indicate that repeated binge EtOH administration during adolescence has long-term consequences on EtOH consumption in adulthood. The present study was conducted in male rats only. Therefore, future work should assess sex-related differences in this pattern of behavioral responding to repeated binge EtOH during adolescence and its long-term consequences.

Abstract #: A-65 Presented by: Kathleen Armstrong, PhD, Faculty Relationship Between Sleep Disorders and Obesity in Preschool Children Kathleen Armstrong, PhD, Casey Gooden, MS 2,USF COM, Kyle Popkave, MA,USF, Psychological and Social Foundations, Carol Lilly, MD,USF COM, Department of Pediatrics Keywords: pediatrics obesity sleep disorders apnea Objective: Study examined prevalence rates for and relationships of overweight and risk of sleep disorders in a sample of 115 children, ages 2-5. Methods: Childen were screened using the Sleep Disorders Inventory for Students, Children’s Version (SDIS-C) and body mass index (BMI). Weight categories were calculated using the Centers for Disease Control and Prevention BMI-for-age growth chart, and consisted of underweight (below the 5th percentile), healthy weight (5th to 85th percentiles), at risk for overweight (85th to 95th percentiles), and overweight (above the 95th percentile). Data were analyzed using the Statistical Package for the Social Sciences (SPSS) software (Version 14, 1999). Results: There was no significant relationship found between weight category and sleep problems or sleep apnea. Conclusion: Future research should compare the relationship between overweight and sleep disturbances in different age groups with samples more representative of the general population. Further, research should examine the accuracy of sleep disorder screening tools, as well as examine the relationship between diagnosed sleep disorders, body weight, and other risk factors in young children. Research supported by: MS 1 Summer Research Stipend Division of Child Development

Abstract #: A-66 Presented by: Ellen Daley, PhD, Faculty HPV Knowledge Among Men Participating in a Psychosocial Study Ellen Daley, PhD, Vamos, C., MPH; Kolar, S., MPH; Baldwin, J., PhD; Buhi, E., PhD, Zaki, R., MPH, Anstey, E., MA, & Chandler, R., MS, FNP-BC USF COPH Department of Community & Family Health Keywords: HPV, knowledge, males Objective: Human Papillomavirus (HPV), the most common sexually transmitted virus, is associated with various anogenital cancers in males and females. However, because no HPV screening test is routinely available for males, little is known regarding their level of knowledge of the virus. The purpose of this study is to determine HPV knowledge among men participating in a psychosocial study of HPV. Methods: Men completed 20 knowledge questions via a computer-assister-survey instrument post-HPV diagnosis. Frequencies were tabulated for each knowledge item in addition to a composite knowledge score. Results: One-hundred and six men completed the survey; most were white and college students. The majority of the men accurately answered certain items correctly, such as “You can have HPV without knowing it” (n=105; 99%); whereas the majority of men inaccurately answered other items, such as “HPV can affect a woman’s ability to get pregnant” (n=22; 20%). The knowledge score ranged from 9 to 20 out of 20, with a higher score indicating higher knowledge. HPV+ men had significantly higher knowledge score compared to HPV– men (p = .02). Conclusion: This study provided a unique opportunity to determine men’s knowledge about HPV. It is important to ascertain the level of HPV knowledge among men so educational materials can be developed with the ultimate goal of decreasing the morbidity and mortality associated with HPV among men and women. Research supported by: National Institutes of Health

Abstract #: A-67 Presented by: Candace Greenough, Undergraduate A Doctor’s Dilemma: Advance Directives Candace Greenough, Aliyah Baluch, MD Sateesh R. Prakash, MD USF CAS Department of Chemistry Keywords: advance directives, culture, ethnicity Objective: Advance directives are an essential aspect of end-of-life care (ELC). An advance directive discusses a patient’s wishes in end-of-life situations. Healthcare providers frequently encounter challenges when addressing ELC in hospitals. Currently, there is sparse information regarding ELC and ethnic background. Our study compared advance directives in two ethnic groups: African Americans and Asians. Methods: We conducted a comprehensive literary search using information from 1999-2007 to review literature discussing advance directives. We reviewed articles discussing advance directives in the African American population. We also reviewed articles that focused on Asian views on ELC. Our Asian population included: Filipino, Chinese, Japanese, and Korean Americans. We then developed a comparison of the two ethnic groups. Results: Less than 30% of Americans as a whole have an advance directive. Our study reveals similarities and differences between both groups. We found that both African Americans and Asians did not have a working knowledge of advance directives. African Americans wanted life-sustaining technology but were unwilling to complete an advance directive. Advance directives in the Asian population were not based on individual autonomy but rather family wishes. Due to cultural factors, Asians were more likely to allow family to decide advance directives. Conclusion: A fundamental understanding of end-of–life goals is a hallmark of patient care. A greater emphasis should be placed on educating both Asians and African Americans on advance directives. Cultural beliefs should be considered in discussing end of life care.

Abstract #: A-68 Presented by: Cecilia Jevitt, PhD, Faculty Biobehavioral Correlates of Obesity in Postpartum Women Cecilia Jevitt, PhD, Maureen W. Goer, PhD, RN USF CON Keywords: obesity, postpartum, stress, cortisol, psychoneuroimmunology Objective: To examine relationships between obesity, as defined by body mass index, and immune, endocrine, stress factors in postpartum mothers. Methods: Data were collected on 200 postpartum mothers in southeastern American between 2002 and 2005. IRB approval and informed consent were obtained. Participants recruited after normal term births were visited at home between 7 and 11 AM at 4-6 weeks postpartum. A venipuncture was performed for serum, cells, and plasma. An unstimulated 5 minute saliva sample was collected. Mothers completed a battery of demographic, stress, health and mood instruments. Flow cytometry of lymphocyte subsets, proliferation assays, stimulated whole blood cultures, serum levels of hormones and cytokine concentrations were done. Results: Obese mothers (36%) were younger, of lower socioeconomic status, more likely to smoke, have low exercise levels, longer labors and more Caesarean births. Anger, depression, and anxiety were higher in obese mothers, as was perceived stress. Obese mothers reported more sicknesses for themselves and their infants. The obese had lower Helper/Suppressor ratios, higher Natural Killer Cell counts, higher levels of Interleukin-6, TNF-á and C-reactive protein, and lower levels of Interleukin-2 and Interferon-gamma, with a greater T cell mitogen response. Serum prolactin was lower and fewer obese women were breastfeeding. Plasma ACTH and salivary cortisol levels were lower suggesting a less responsive hypothalamic-hypophyseal axis. Conclusion: Obese postpartum mothers have a greater activated inflammatory state than normal weight mothers and a depression in cellular immune processes. This may account for their increased postpartum illness and dysphoric moods. Research supported by: National Institute of Nursing Research

Abstract #: A-69 Presented by: Rebecca Kukla, PhD, Faculty Pregnant Women's Participation in Online Communities Rebecca Kukla, PhD, USF COM Department of Internal Medicine Keywords: pregnancy, Internet, health communication, prenatal care Objective: 1. Explore how pregnant women use online communities as sources of medical information and social support. 2. Understand how the Internet is changing the traditional provider-patient relationship during prenatal care. Methods: I conducted extensive, open-ended interviews online with 16 pregnant or recently pregnant Canadian and American women who currently participate in pregnancy-related online communities. The women ranged in age from 16 to 41 and came from diverse socioeconomic, religious, and cultural backgrounds. Results: The interviewees found online communities to be valuable sources of health information, health advice, and social support. Women who experienced serious pregnancy complications found the online communities particularly valuable. Most women demonstrated an ability to critically assess both the information they found online and the information they received from their doctors. Many were critical of other women who accepted the word of either their providers or their online peers at face value, without further research. Many women continued participating in the online communities long after their children were born. Most women expressed substantial dissatisfaction with the attention they received from their primary health care providers. Conclusion: The study indicates that the Internet is having a profound impact on how pregnant women find and assess health information, and on their relationships with their providers. This is especially so for women who experience complications during pregnancy. Ethical analyses of prenatal care practices must take the role of Internet communities in patients' lives into account. Research supported by: Social Science and Humanities Research Council of Canada

Abstract #: A-70 Presented by: Karen Liller, PhD, Faculty The Development of the Sports Medicine and Athletic Related Trauma (SMART) Sports Injury Registry for Adolescents Karen Liller,Barbara Morris, SMART, Jeff Konin, SMART, Micki Cuppett, USF COM, Siwon Jang, COPH, Stephen Thorson, Simtrak TM USF COPH, Department of Community & Family Health Keywords: injury prevention, sports, adolescents, athletics Objective: Describe the methodology surrounding the development of the Sports Injury Registry. Explain the unique features of the registry that will allow for the development of targeted sports injury prevention efforts. Demonstrate the software at USF Health Research Day. Methods: The foundation of the registry was developed utilizing professional sports injury surveillance software created by SimtrakTM. Our new variables focused on exposure (number of athletes participating in the number of organized practices and/or games), race/ethnicity, detailed injury mechanisms and activities, field locations and positions, illegal/foul play, time of injury, environmental conditions, concussion variables, protective equipment, and outcomes. Ten certified athletic trainers (ATCs) have been hired by SMART to provide care in 10 public high schools in the region. The software has been installed on the ATC’s laptops so they can collect data during practices and events. Once a week, the data are exported to the university for analysis. Results: Data have been collected from all 10 schools from August-December. Data will be analyzed with SPSS to determine frequencies and relationships between injuries and exposure, demographic, behavioral, and environmental factors. Conclusion: The investigators will demonstrate the software at Research Day. The injury data collected from this project will lead to a more accurate understanding of sports injury rates, significant risk factors, and how to target ecological interventions. Research supported by: Sports Medicine and Athletic Related Trauma Institute of the Department of Orthopaedics & Sports Medicine at the University of South Florida College of Medicine.

Abstract #: A-71 Presented by: Alejandro Mendoza, Undergraduate Type II Diabetes in Children: A Growing New Epidemic Alejandro Mendoza, Sateesh R.Prakash, M.D. Aliyah Baluch, M.D. USF CAS, Department of Chemistry Keywords: children, age, ethnicity, race and DM II Objective: Type II Diabetes (DM II) is a disease that usually affects the adults. Recently, a growing number of children have developed symptoms DM II. There is sparse data evaluating the factors that are involved with this epidemic. We evaluated the factors that pre-dispose children towards obesity therefore increasing the risk of DM II. Methods: A literary search found articles between1997-2007 analyzing socio-economic factors and ethnicity in children and adolescent. Keywords used were children, age, ethninicity, race and DM II in order to asses for an emerging pattern in DM II. Results: Pediatricians have noted an increase in DM II in children of all ethnic groups but the greatest increase is in non-Caucasians. Specifically American Indian children (in Montana and Wyoming) had the greatest prevalence of DM II. Hispanic and African American children have similar issues. Of the socio-economic issues, the degree of poverty has the greatest impact on the health of the children. These issues are linked to an elevated Body Mass Index (BMI). Cross-sectional surveys have demonstrated a link between DM II and hyperlipidemia in Canadian Indian pediatric populations and also in the children and adolescents of Florida. The trends of DM II among children receiving Medicaid in Kentucky between 1994 and 2001 demonstrated genetic factors, ethnicity, and lifestyle as factors responsible for the epidemic. Conclusion: DM II has become a national health epidemic in youth. Research and health education should reflect that ethnic and socio-economic factors can change the face of childhood which has ramifications that physicians will deal with for generations to come.

Abstract #: A-72 Presented by: Crystal Wilson, Undergraduate Attitudes Toward the use of PGD for BRCA1/2 Mutations Among Concerned Women Crystal Wilson USF CAS, Department of Psychology,Susan Vadaparampil(1,2), Lindsey King(2), Cheryl Miree(2), Gwendolyn Quinn(1,2) (1)USF College of Medicine, Department of Interdisciplinary Oncology (2)H. Lee Moffitt Cancer Center and Research Institute, Health Outcomes and Behavior Program Keywords: BRCA1/2, Preimplantation Genetic Diagnosis, Attitudes, Knowledge Objective: Recent studies show women who carry a BRCA1/2 mutation or are high-risk for Hereditary Breast and Ovarian Cancer (HBOC) may be interested in using Preimplantation Genetic Diagnosis (PGD) to avoid passing along a genetic mutation to their future children. This study seeks to determine if concerned women are aware of PGD and its use, are favorable toward the use of PGD for HBOC, and likely to consider using it personally. Methods: A 29-item survey was distributed to women attending the 2007 Joining FORCEs Conference held by FORCE (Facing Our Risk of Cancer Empowered) in Tampa, FL. The survey was created to determine the knowledge and attitudes of women affected by HBOC towards PGD. A brief explanation of PGD was provided in the survey. Responses were analyzed and summarized using descriptive statistics. Results: A total of 111 eligible women completed the survey. Only 31% of the women surveyed had heard of PGD prior to the administration of the survey. Fifty-six percent felt PGD was an acceptable option for women with high risk for HBOC. About 75% felt high risk individuals should be given information about PGD. Most women, 42%, said they would not consider using PGD themselves, while 30% said they would. None of the women surveyed had ever used PGD. Conclusion: As a whole, concerned women feel that PGD is an acceptable option for individuals with a history of breast or ovarian cancer. Most, however, would not consider using it themselves and most had not heard of PGD. High risk women want to be informed about PGD. Therefore, more research is needed on the educational and psychosocial needs of women high-risk for HBOC. Research supported by: American Cancer Society [RSGPB-07-019-01-CPPB]

Abstract #: A-73 Presented by: Kimberly Badanich, MA, Graduate Student The Reinstatement Of Place Conditioning In Rats Exposed To Cocaine During Adolescence Kimberly Badanich, MA,,USF, COM, Cheryl L. Kirstein, Ph.D 1 2 1. Department of Psychology, College of Arts and Sciences 2. Department of Molecular Pharmacology and Physiology, COM, USF Keywords: dopamine, nucleus accumbens, ventral tegmental area, adolescent rat, cocaine Objective: Psychostimulant-induced reinstatement of place preferences have been used to investigate underlying physiological mechanisms mediating drug-seeking behavior in adolescent and adult rodents; however, it is still unclear how psychostimulant exposure during adolescence affects neuronal communication in the mesolimbic DA pathway and whether these changes would elicit enhanced drug-seeking behavior later in adulthood. The aim of the present study was to investigate the effects of intra-ventral tegmental area (VTA) or intra-nucleus accumbens septi (NAcc) dopamine (DA) D2 receptor antagonist infusions on cocaine-induced reinstatement of cocaine place conditioning. Methods: Beginning on postnatal day (PND) 28, each rat was exposed to 7 different phases including 1) handling 2) baseline chamber test 3) acquisition of cocaine place conditioning (20 mg/kg/ip) 4) expression of cocaine place preference 5) maintenance and extinction of cocaine place preference 6) implantation of guide cannula into either the VTA or NAcc and 7) infusion of the D2 DA receptor antagonist sulpiride (100uM; PND 70). Results: Data show the impact of locally infused D2 antagonist into the VTA or NAcc on the reinstatement of cocaine place preferences. Conclusion: These data suggest intrinsic compensatory mechanisms in the mesolimbic DA pathway mediate adolescent behavioral responsivity to cocaine prime-induced reinstatement of cocaine place preferences in adulthood. Research supported by: RO1DA14024

Abstract #: A-74 Presented by: Huayan Hou, MD, Postdoc Modulation Of Neuronal Differentiation By CD40 Isoforms Huayan Hou,Demian Obregon, Kavon Rezai-zadeh, Frank Fernandez Archie Silver and Jun Tan USF COM, Department of Psychiatry Keywords: CD40 isoforms, CD40L, neuronal differentiation Objective: we expect differential expression of CD40 isoforms type I and type II in neurons may modulate neuron differentiation. Methods: In this study, immunohistochemistry and immunocytochemistry staining were used to detect the differential CD40 isoforms type I and type II in neuron differentiation, and it was confirmed by western blot. Results: Results show that in CD40-/- deficient mice express predominantly type CD40 type I and II, and these mice displayed reduced CD40 type I expression and slightly less type II expression compared with wild-type age and gender matched controls. Younger 1 day old wild-type mice displayed less CD40 type I, and more CD40 type II, and soluble CD40 (CD40 pathway antagonist molecule) compared with older 12 month old mice. Neuron-like N2a cells express CD40 type I and type II isoforms, and displayed more CD40 type I in when differentiated, and less CD40 type II compared with the undifferentiated state. Conclusion: these data suggest that CD40 isoforms may modulate neuron differentiation, and CD40 type I may enhance or help maintain the differentiated state in neurons.

Abstract #: A-75 Presented by: Jeffy Jimenez, MS, Graduate Student Effects of Anti-Aß Antibodies against Aß Peptide Aggregates: Brain Clearance of Alzheimer’s Disease Jeffy Jimenez, MS, David Morgan (Department of Molecular Pharmacology & Physiology), Norma Alcantar (Department of Chemical Engineering), USF College of Engineering Chemical Engineering Keywords: Amyloid Beta, AFM, ATR-FTIR, Immunotherapy, Fibrils Objective: Alzheimer’s disease (AD) is a neurodegenerative disease which gradually destroys the affected patient’s brain. Three different hallmarks are known: the deposition of extracellular amyloid beta peptide plaques, the intercellular formation of tau protein tangles, and finally the neuronal death. This work is oriented to test different antibodies and to find the optimal amounts to be used in the clearance of Aß peptide deposits. It is desired to determine the dissolution effects of different antibodies over Aß peptides aggregates and to account for the molecular conformation changes provoked by these antibodies over the peptide molecules during this process. Methods: The antibodies tested have different affinities to either terminus C, N, or to the mid domain of the amyloid beta peptides 1-40 and 1-42. Two main analytical techniques are used in this study: Atomic force microscopy (AFM) to characterize the physical change of the peptide aggregates with time; and attenuate total reflection Fourier transform infrared (ATR-FTIR) spectroscopy to characterize the temporal conformational structures and changes of the peptides molecules. Results: These two techniques allowed monitoring the aggregation processes of these peptides and the relationship of their dissolution to the addition of anti-Aß antibody dosages. Conclusion: Our results include the optimization of antibody quantities used over different mixtures of Aß peptides to dissolve the plaques that may cause minimum secondary effects to patients. Research supported by: *Internal Award 2006. RO 488 College of Engineering. University of South Florida, Tampa FL. *Pfizer Grant (Project Number: 2107102400). *NSF-IGERT (grant No., DGE-0221681) program. *Alfred P. Sloan Foundation.

Abstract #: A-76 Presented by: John Koren, Undergraduate Akt and CHIP Co-Regulate Tau Degradation Through Coordinated interactions John Koren, Chad A Dickey, Umesh K Jinwal, Dave Morgan, USF COM, Department of Molecular Pharmacology and Physiology, Cam Patterson, University of North Carolina at Chapel Hill, Chapel Hill, NC, Leonard Petrucelli, Mayo Clinic Jacksonville, Department of Neuroscience, Jacksonville, FL USF CAS, Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: TAU AKT CHIP PAR1/MARK2 Objective: Recent studies suggest that the accumulation of the microtubule-associated protein tau into fibrillar aggregates, hallmark of the pathology of Alzheimer's disease, is because cytosolic chaperones fail to clear abnormally phosphorylated tau, preserving a pool of toxic tau intermediates within the neuron. We describe a novel mechanism for tau pathology involving a major cellular kinase, Akt. Methods: Standard Western blot and co-immunoprecipitation analysis of transfected cell lysates and knockdown mouse brain tissue were used to investigate protein levels and view protein interactions. Results: Akt prevented CHIP-induced tau ubiquitination and its subsequent degradation. Akt levels tightly regulated the expression of CHIP, such that as Akt levels were supressed, CHIP levels also decreased, suggesting a potential stress response feedback mechanism between ligase and kinase activity. We also showed Akt and the microtubule affinity regulating kinase 2 (PAR1/MARK2), a tau kinase, interacted directly. Overexpression of PAR1/MARK2 prevented the binding of Akt to both tau and CHIP. Akt enhanced the activity of PAR1 to promote tau hyperphosphorylation at S262/S356, a tau species not recognized by the CHIP/Hsp90 complex. Also, Akt1 knockout micehad reduced levels of tau phosphorylated at PAR1/MARK2 consensus sites. Conclusion: Thus, Akt serves as a major regulator of tau biology by manipulating both tau kinase and protein quality control. We now have new evidence suggesting that tau may facilitate the interaction of Akt with the chaperone complex, demonstrating a novel function for the tau protein beyond microtubule stabilization. Research supported by: Alzheimer's Assocition NIA CurePSP AFAR

Abstract #: A-77 Presented by: Daniel Lee, PhD, Postdoc Selective Gene Expression Following Classical or Alternative Activation of Microglial in Mouse CNS Daniel Lee, Lori Lebson USF COM, Dave Morgan, Marcia Gordon USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Neuroinflammation, Microglia, cytokines, mouse Objective: The role of microglia during neuroinflammation remains controversial due to various expression markers, activation states, and stimuli. At least two activations states have been suggested consisting of a classical response (M1) comprised of tissue damage, inflammation, and pathogen destruction, whereas the alternative response (M2) leads to tissue repair and suppression of inflammation. Identifying biomarkers of microglia representative of these activation states may help elucidate disease course and understand repair mechanisms. Methods: We performed intraparenchymal injections into the hippocampus (HPC) with different cytokine cocktails designed to selectively elicit either an M1 or M2 state into 12month old mice. The M1 cocktail contained TNF-?/ IL-12/ IL-1?, whereas the M2 cocktail consisted of IL-13/ IL-4. To identify novel and more selective markers, microarray analysis was performed on HPC tissue 3 days post injection. Results: Gene activation was compared between M1 vs M2 stimulator cocktails; putative selective M1 activation markers were induced by the M1 cocktail by at least 2-fold greater than by the M2 cocktail, while putative M2-responsive genes were induced by the M2 cocktail to at least twice the level induced by the M1 cocktail. Several top genes expressed for the M1 cocktail included CXCL13, haptoglobin, and MARCO, while selective genes induced by the M2 cocktail consisted of FIZZ1, Spred2, and EAR 11. Conclusion: Real-time PCR will be used to verify selected genes. These data reveal several potential markers that represent selective M1 vs. M2 activation. Future studies will determine whether and how activation through these diverse pathways results in differences in microglial functions. Research supported by: P01 AG 04418, R01AG 15490, R01 AG18478

Abstract #: A-78 Presented by: Quinn Lunquist, Undergraduate Neurotoxic Effect of Prenatal Exposure to Flame Retardant in the Rat on Cerebellar Purkinje Cells Quinn Lunquist,,PR Kodavanti, Div. Neurotoxicology, USEPA, Research Triangle Park, NC; RF Mervis, Dept Neurosurgery/Center for Aging and Brain Repair, USF COM and Neurostructural Research Labs, Tampa, USF COM Department of Neurosurgery Keywords: neurotoxicology, brain development, cerebellum, dendrites, Golgi stain Objective: Polybrominated diphenyl ethers (PBDEs) are widely used commercial brominated flame retardants that are accumulating in the environment. PBDEs may interfere with the development of key biological systems, thus leaving children vulnerable to functional impairments in adulthood. This study evaluated the effects of in utero exposure to PBDE (at two dose levels) on Purkinje cell (PC) dendritic branching and spines in the juvenile and adult rat cerebellum. Methods: Pregnant rats were administered PBDE from gestational days 6-21 with vehicle (corn oil), 10.2 mg/kg/day (mid dose) or 30.6 mg/kg/day (high dose)of DE-71. Rats were sacrificed on postnatal days 22 and 60. Cerebella were Golgi stained, all slides were coded, and dendritic branching areas and spines of randomly selected Purkinje cells were quantified. Results: Compared to age-matched controls, the high dose of PBDE resulted in a small decrease in PC branch area in both 22 and 60do rats with no change in spine density. At both ages, the mid-dose of PBDE , however, resulted in a modest increase in PC branch area accompanied by a dramatic and highly significant loss of dendritic spines. Conclusion: The paradoxical nature of the response of Purkinje cell branching and spines to PBDE administration – e.g., a large neurotoxic effect of the mid-dose exposure to PBDE and negligible effect of the high PBDE dose – is currently not understood and is under intense investigation. However, the finding suggest that even small levels of exposure to brominated flame retardants may have deleterious and long-lasting consequences on brain circuitry in the juvenile and adult mammalian brain. Research supported by: USEPA

Abstract #: A-79 Presented by: Elona Rrapo, Undergraduate HIV-1 Tat Contributes to Alzheimer's-like Pathology in PSAPP Mice Elona Rrapo, Yuyan Zhu, Houyan Hou, Jun Tan , and Brian Giunta, Neuroimmunology Laboratory, Institute for Research in Psychiatry, Department of Psychiatry and Behavioral Medicine , USF CAS Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: HIV, Tat, Alzheimer's, Dementia, PSAPP Objective: Long-term HIV survivors develop Alzheimer's disease (AD)-like pathology and antiretroviral therapy (ART) has increased the prevalence of HIV associated dementia (HAD). HIV-induced amyloid- beta (Ab) deposition and excitotoxicicty of Tat protein are risks for AD and/or HAD development. To address this, an animal model, to study HIV-1 Tat induced AD-like pathology for drug development was created. Methods: A dose ranging study (54, 108, and 216 mg/kg/day x 7 days) was performed to examine neuron loss (H&E stain) and tau phoshyphorylation (ELISA) in Doxycycline (Dox) inducible, HIV-1 Tat transgenic mice ( N=16; a previously validated HAD model). Next PSAPP (N=5; a previously validated AD model) and Tat transgenic (N=5) mice were crossed, offspring analyzed via PCR, and ELISA, Western Blot, and antisera detection for Ab1-40,42 was conducted. Results: Dox (54mg/kg daily, every other week x 8 weeks) dose-dependently induced neuron loss and tau phosphyrlation in all Tat transgenic mice. Three PSAPP/Tat-Transgenic mice (N=3) carrying the “Swedish" APP mutation, the mutant presinilin 1 gene, and an HIV-1 Tat gene controlled by a Dox inducible promoter resulted from crosses of PSAPP and Tat transgenic mice. These, compared to PSAPP mice (exposed to Dox [negative control]), and PSAPP/Tat-transgenic (not exposed to Dox [negative control]), showed significantly more Ab1-40,42 deposition in brain regions examined. Conclusion: In the future long-term HIV survivors on HAART who develop AD-like pathology will likely demonstrate pronounced neurofibrillary tangles, Ab plaques, and neuron loss in post-mortem brain. This model will serve as a basis to study HIV induced AD-like cognitive deficits and response to therapy. Research supported by: NIH/NINDS/NIMH

Abstract #: A-80 Presented by: Andrea Schlunk, Undergraduate Fractalkine Receptor CX3CR1 Regulates Adult Hippocampal Cell Proliferatio A. Schlunk1, A. Wingert.2, A.D. Bachstetter3, M.J.Cole,3 C.E. Hudson4, M. J. Ehrhart, 4 Fister,4 , P.R. Sanberg3, P.C. Bickford3,5 and C. Gemma,3,5 1 Honors College USF, 2CAS, USF, 3 Center of Excellence for Aging and Brain Repair, Dept. of Neurosurgery, USF, 4 Siver Child Development Center, USF, 5 James A. Haley VA Hospital, Tampa, FL,USF CAS, Department of Biology - Integrative Biology Keywords: aging, inflammation, neurogenesis Objective: Adult hippocampal neurogenesis dramatically decreases with increasing age. Microglia are the resident immune cells in the central nervous system. However, activation of microglia, as is the case in the aged brain, has been shown to contribute significantly to the decrease in neurogenesis associated with aging. Fractalkine is a chemokine principally expressed in neurons, while its receptor, CX3CR1, is present on microglia cells. Recently, it has been shown that CX3CR1 is critical for regulating the function of microglia and that its ablation induces excessive microglia activation in response to inflammatory stimuli. The object of this study was to investigate whether an impaired CX3CR1/fractalkine function in the central nervous system may modulate the decrease in hippocampal cell proliferation associated with aging. Methods: Here, we analyzed cells positive for BrdU in young and aged rats. Anti-rat CX3CR1; rabbit IgG; heat-denatured fractalkine; or naïve fractalkine were infused for 7 d through an osmotic minipump connected to a brain infusion cannula implanted into the left lateral ventricle. The rats then received BrdU (50 mg/kg i.p.) on day 8 to label proliferating cells. Results: Unbiased stereology analyses revealed that the blockade of CX3CR1 function in the young rats significantly decreased the number of proliferative cells in the dentate gyrus when compared to control. Interestingly, the blockade of CX3CR1 did not affect cell proliferation in the aged animals. Conclusion: These findings indicate that CX3CR1 is essential for regulating adult hippocampal cell proliferation. Research supported by: National Institutes of Health (AG024165A, AG04418) and the VA Medical Research Service.

Abstract #: A-81 Presented by: Ashley Sponaugle, BA, Undergraduate Effects of Adolescent MDMA on Conditioned Place Preference and Neurogenesis Ashley E. Sponaugle, B.A., USF CAS Department of Psychology, Briony J. Catlow, M.A., Kimberly A. Badanich, M.A., A.B.D.1, Cheryl L. Kirstein, Ph.D.1.2, Department of Psychology, Cognitive and Neural Sciences, USF 2Department of Molecular Pharmacology and Physiology, USF COM and Juan Sanchez-Ramos, M.D., Ph.D.2 3, 3 Department of Neurology and Department of Psychiatry and Behavioral Medicine, USF COM Keywords: MDMA, CPP, Neurogenesis, Adolescence Objectives: Adolescence is typically the period when drug abuse is initiated and is often defined by high risk taking, exploration, and sensation seeking. Drugs of abuse are believed to have a marked effect on neurogenesis in the adolescent brain. MDMA abuse in human adolescence is prevalent; moreover, abuse during this time period may impact normal development of the brain or potentiate the likelihood of subsequent drug use/abuse. The aim of the present study was to 1) evaluate the effects of MDMA in adolescent rats using the conditioned place preference (CPP) paradigm to measure MDMA-induced reward and 2) to measure effects of MDMA on neurogenesis in the adolescent hippocampus. Methods: MDMA CPP was measured in early adolescents [postnatal day (PND) 28-39] by training rats to associate 1.25, 2.5, 5.0 mg/kg/ip MDMA or saline with environmental cues. After drug cessation (24 hr or 2 weeks) Bromodeoxyuridine (BrdU) was injected followed by euthanasia. Unbiased counts of the number of BrdU, doublecortin and calretinin positive cells in the dentate gyrus were estimated. Results: Data show MDMA-induced place conditioning effects at several doses. Conclusions: Ontogenetic differences in MDMA-induced neurogenesis may mediate dose response effects in MDMA-induced place conditioning in adolescent rats.

Abstract #: A-82 Presented by: Chung To, Undergraduate Myelin Fatty Acid Content Altered in Brain Cortex and Hippocampus of Hyperglycemic Rats Chung To, Suzan K. Hanna, John I. Malone USF COM/Dept. of Pediatrics, Samuel Saporta USF COM/Dept. of Pathology and Cell Biology Ronald F. Mervis, USF COM/Center of Excellence for Aging and Brain Repair, Department of Neurosurgery Collin R. Park, David M. Diamond USF COM/Departments of Psychology, Molecular Pharmacology and Physiology, and Medical Research, VA Hospital, USF CAS, Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: hyperglycemia, myelin, fatty acids neuronal changes Objective: To determine how the Fatty Acids (FA) production or transport is affected by hyperglycemia and their function on the central nervous system. Methods: 4-week old male Wistar rats were made hyperglycemic with streptozotocin. Daily blood glucose >200 mg/dl and terminal HbA1c >9% differentiated the hyperglycemic animals (Hyper)(26) from the Control rats(Con)(21)(bG Results: Total lipid, Phospholipid, & Cholesterol were the same in the Hyper and Con brains. Palmitoleic, vaccenic, ?-linolenic,& oleic acids of myelin in Hyper were different from Con in the cortex and hippocampus.The FA levels are important for the normal structure and function of neuronal dendrites and axonal myelin. Thus Hyper altered either FA production or transport in the brain of these animals. Glucose metabolism appears to be different in these 2 areas of the central nervous system since the sorbitol content in the cortex of hyperglycemic rats (7.5 ± 1.6 µM/mg protein) was less than found in the hippocampus (17.2 ± 3.3 µM/mg protein) of the same animals. The Hyper sorbitol levels in the cortex and hippocampus were different from Con. Conclusion: Myelin FA content also differed in Hyper from Con but in unique ways for the cortex and the hippocampus. These differences may explain the changes noted in neuronal structure and function of these animals. These observations support the need for greater interest in the impact of chronic Hyper upon the function of the central nervous system. Research supported by: Juvenile Diabetes Research Foundation Granat (1-2004-751) and Julia Parrish Diabetes Research Endowment

Abstract #: A-83 Presented by: Winay Virkud, BS, Undergraduate Heavy Particle Radiation Alters Neuronal Morphology – Implications for Interplanetary Spaceflight Winay Virkud, K. Kasimos (USF Honors College), I. Quasem (USF Graduate School, Dept Microbiology), B. Rabin, PhD (Dept Psychology, Univ Maryland - Baltimore County), R.F. Mervis, PhD (USF College of Medicine, Dept of Neurosurgery, Ctr for Aging & Brain Repair and Neurostructural Research Labs, Tampa, FL) Keywords: Dendrites, Brain Injury, Radiation, Synapses, Neocortex Objective: In future deep space missions, astronauts will be exposed to heavy particle radiation over lengthy periods. This exposure could result in neuronal damage, impair brain circuitry, compromise the astronaut’s cognitive capabilities and threaten mission success. Relatively little is known of the effect of this exposure on neuronal circuitry. The purpose of this study was to evaluate the effects of high-energy (56)Fe particles on dendritic branching and spine (synaptic) parameters in cortical and hippocampal regions of the adult rat. Methods: 4 month-old Sprague-Dawley rats were exposed to a single exposure of (56)Fe radiation (1.5Gy of 1GeV/n). 28 days later the animals were sacrificed. Formalin fixed coronal tissue blocks were stained using the rapid Golgi method. This technique shows the dendritic branching and spines of impregnated neurons. From coded slides, randomly selected layer II-III pyramids of the frontal cortex and granule cells of the hippocampus were evaluated. Camera lucida drawings were assessed using Sholl analysis show the amount and distribution of the dendritic tree and dendritic spines were counted. Results: There was a significant loss of dendritic material (~10%) in the basilar dendritic arbor of the cortical neurons exposed to the heavy particle irradiation. Paradoxically, granule cells showed a significant increase in dendritic material (+ 20%). Spine changes were minimal but most affected spines believed related to memory. Conclusion: The findings suggest that exposure of the rat brain to irradiation equivalent to that which astronauts might encounter on deep space missions may have deleterious effect on neuronal morphology and brain circuitry. Neuroprotective strategies may be needed for mission success. Research supported by: NASA (awarded to B.R.)

Abstract #: A-84 Presented by: Tracy Womble, PhD, Postdoc CD14+ Human Umbilical Cord Blood Cells are Essential for Neurological Recovery Following MCAO Tracy Womble, Suzanne Green, Department of Neurosurgery, Center for Aging and Brain Repair, USF, Paul R. Sanberg, Department of Neurosurgery, Center for Aging and Brain Repair, USF, Keith R. Pennypacker, Department of Molecular Pharmacology and Physiology, USF COM, Alison E. Willing, Department of Neurosurgery, Center for Aging and Brain Repair, USF COM Department of Neurosurgery Keywords: human umbilical cord blood, middle cerebral artery occlusion, monocyte, stem cells, macrophage Objective: When human umbilical cord blood (HUCB) cells are administered following middle cerebral artery occlusion (MCAO) in the rat, they induce motor recovery and decrease infarct size. Because few cells are found at the site of injury and alterations in pro- and anti-inflammatory cytokines, we believe it is an immune/inflammatory response regulating recovery following HUCB treatment. The HUCB cell preparation is a mixed population composed of immature T-cells, B-cells, monocytes/macrophage and stem cells. In this study we examined whether the beneficial effects of HUCB injection can be attributed to a specific cell population. Methods: Male Sprague Dawley rats underwent permanent MCAO followed 48 hours later by i.v. administration of HUCB preparations from which selected immune cell populations had been depleted. Thirty days post-stroke, a battery of behavioral tests were performed to assess functional recovery. Results: On the step test of motor asymmetry, we found that the whole mononuclear fraction of cord blood, stem cell, T-cell and B-cell depleted preparations improved function of the impaired left forelimb to a similar extent that exceeded performance by MCAO only animals. Animals that received the monocyte/macrophage depleted HUCB preparation performed more poorly than those receiving the other HUCB cells. When we examined spontaneous activity, MCAO lesioned animals became significantly hyperactive. Conclusion: Consistent with earlier studies, HUCB administration significantly reduced this hyperactivity. Depletion of stem cells, monocytes (CD14+) and B-cell prevented this recovery, suggesting these cells are critical to HUCB-induced recovery following stroke. Research supported by: Supported by: NINDS R01NS52839 (AEW)

Abstract #: A-85 Presented by: Jeremy Yesudas, Undergraduate Persistent Hippocampal Dendritic Alterations Following Prenatal Exposure to a Cannabinoid Agonist Jeremy Yesudas,,N. Vyas (USF Honors College), S. Moradian (USF Honors College), B. Thomas (USF Honors College), L. Nattkemper (USF Graduate School, Dept. Biomedical Sciences), V. Cuomo/Univ La Sapienza Rome-Italy, T. Cassano/Univ Foggia-Italy, S. Gaetani/Univ La Sapienza Rome-Italy, RF Mervis/Center for Aging & Brain Repair-USF College of Medicine USF COM, Department of Neurosurgery Keywords: brain development, dendrites, dendritic spines, drugs of abuse, marijuana Objective: Among women of reproductive age marijuana is the most widely used illegal drug. Although marijuana can affect learning and memory of adult users, the impact of cannabis exposure on the developing brain is poorly understood. A specific cannabinoid receptor (CB1) is highly expressed in many brain regions, including the hippocampus. The objective of this study was to assess the effects of prenatal exposure to a synthetic CB1 receptor agonist (WIN55 212-2) on neuronal morphology of granule cells in the dentate gyrus in the juvenile and adult rat. Methods: WIN55,212-2 administered daily from gestational day (GD) 5 to GD 20 (0.5mg/kg). ). This dose is equivalent to a moderate or low exposure of marijuana in humans and has no overt toxic effects. Offspring and age-matched controls were sacrificed at 40- and 80-days-old. Brain tissue was Golgi impregnated (which permits assessment of dendritic branching and spines). From coded slides, the dendritic branching and spines of granule cells were quantified. Results: In the 40 d.o rats, Sholl analysis showed dendritic branching of the granule cells of the WIN-exposed rats was significantly greater than age-matched controls. This effect of prenatal WIN-exposure still persisted in the 80 d.o. rat granule cells. Branching complexity was also enhanced. Initial evaluation of spines showed more minimal alterations, with a tendency for spines to be reduced in the WIN-exposed rats. Conclusion: These finding suggests that hippocampal circuitry was affected – perhaps due to failure of the normal developmental pruning back of the dendritic tree. Behavioral deficits in children of adults who abused marijunana may be due to a disruption of normal maturation of hippocampal circuitry by prenatal exposure to the cannabinoid.

Abstract #: A-86 Presented by: Yuyan Zhu, PhD, Postdoc CD45RB agonism: A Novel Therapeutic Target To Inhibit AB Peptide Induced Microglial MAPK Activation Yuyan Zhu,Huayan Hou, William V. Nikolic, Jared Ehrhart, Elona Rrapo, Brian Giunta, Jun Tan Rashid Laboratory Developmental Neurobiology, Silver Child Development Center, Department of Psychiatry and Behavioral Medicine, USF COM, Department of Psychiatry Keywords: Protein tyrosine phosphatase (PTP), mitogen activated protein kinae (MAPK), Beta-amyloid, microglia, Alzheimer’s disease (AD) Objective: to investigate effects of CD45RB on microglial phagocytosis of AB peptide and AB peptide induced microglial MAPK activation. Methods: in this study, CD45RB modulation of AB peptide-activated primary cultured microglial cells was investigated. Microglial phagocytosis of AB1-42 peptide was analysed by flurometrics, flurescence microscopy and confocal microscopy, respectively. ELISA was applied to examine TNF-A or IL-6 concentration in cultured supernatants. Phosphorylated P38 and P42/44 MAPK were assayed by Western blot. Results: microglial cells were co-treated with “aged” FITC- AB1-42 and multiple CD45 isoform agonist antibodies. Data revealed cross-linking of CD45RB, enhances microglial phagocytosis of AB1-42 peptide and inhibits LPS-induced activation of p44/42 and p38 pathways. Co-treatment of microglial cells with CD45RB antibodies results in significant inhibition of LPS induced microglial TNF-A and IL-6 release through p44/42 and/ or p38 pathways. Moreover, inhibition of either of these pathways augmented CD45RB cross-linking induced microglial phagocytosis of AB1-42 peptide. To investigate the mechanism(s) involved, microglial cells were co-treated with a PTP inhibitor (Phen) and AB1-42 peptides. Data showed synergistic induction of microglial activation as evidenced by TNF-A and IL-6 release; both of which are demonstrated to be dependent on increased p44/42 and/or p38 activation. Finally, it was observed cross-linking of CD45RB in the presence of AB1-42 peptide, inhibits co-localization of microglial MHC class II and AB peptide; suggesting CD45 activation inhibits the antigen presenting phenotype of microglial cells. Conclusion: agonism of CD45RB PTP activity may be an effective therapeutic target for novel agents to treat AD.

Abstract #: A-87 Presented by: Helen Acree Conlon, MS/MPH, Graduate Student Human Bites in the Classroom: Incidence, Treatment and Complications Helen Acree Conlon, MS/MPH, USF CON Keywords: bites, hepatitis, human, wound care Objective: TO review current literature and report the findings relating to human bites in the classroom and its impact on the role of the school nurse. Methods: Extensive literature review and consolidate findings Results: It has been estimated that at least one half of the population will experience some type of bite in their lifetime. Human bites are the third leading cause of all bites seen in hospital emergency departments after dog and cat bites. Human bites can be the source of exposure to body fluids, transmission of communicable diseases, infections ranging from cellulitis to osteomyelitis, and joint deformity from septic arthritis. Approximately 10–15% of human bites will become infected. In the school setting, a teacher or other staff member can sustain a human bite while trying to protect a student from injury. Often the first responder to this type of incident is the school nurse. The role of the school nurse is to promote early reporting, accurate history taking of the time and mechanism of injury, administering immediate wound care, initiating early referrals to appropriate medical providers, and supporting proactive care in the form of adult hepatitis B immunization. Conclusion: This study is designed to assist the school nurse in identifying the incidence, common location, and types of human bites; educating school employees about the importance of early reporting, assessment,and treatment of human bite wounds; initiating proper wound management strategies to decrease infections; assessing adult vaccination status of teachers and school employees in high-risk areas; and using this opportunity to change a reactive style of nursing care to a proactive style with emphasis on health promotion and protection. Research supported by: ERC student poster

Abstract #: A-88 Presented by: Sophia Debroux, MS, Graduate Student Tiger Underfoot. Sophia Debroux, MS, USF CON Keywords: Chronic Job Stress Compensation Occupational Disease Objective: Nursing, noted to be one of the more stressful professions, illustrates an environment ripe for injury and accidents; occupational diseases; and gender bias regarding compensation laws. The purpose of this poster is to highlight chronic stress issues particular to the nursing profession, emphasizing the role of the health and safety industry, particularly that of the occupational health nurse. Methods: The purpose of this poster is to highlight chronic stress issues particular to the nursing profession, emphasizing the role of the health and safety industry, particularly that of the occupational health nurse. Results: Greater focus is needed to broaden the understanding of the impact of workplace stress and its interplay with the level of individual actualization. Conclusion: In addition, training and education is needed so that effective psychological stress and strain scales can be designed for an organization’s particular needs. In lieu of this, occupational health and safety practitioners are in a unique position to: conduct training sessions for management, supervisors, and workers on health and stress reducing practices; monitor and evaluate the program’s progress, making additional suggestions when needed; investigate unsafe and stressful working conditions, study possible causes, and recommend remedial action. increase worker productivity and reduce absenteeism/turnover; reduce insurance premiums, litigation expenses, and legislate for better workers’ compensation benefits. Research supported by: USF ERC and CDC/NIOSH.

Abstract #: A-89 Presented by: Mary Gallant-Roman, BS, Graduate Student Workplace Violence in the Healthcare Setting Mary Gallant-Roman, USF CON Keywords: Workplace Violence Objective: Nurses are precious resources in today's healthcare economy. Yet, they are at increased risk of experiencing workplace violence. Most incidents go unreported, so that the true magnitude of the problem is unknown. Nurses often feel that violence comes with the job and they have no recourse or support from administration. The purpose of this research is to describe Workplace Violence and identify elements that require additional study. Methods: A research of literature will include only studies performed in the United States. Results: Research on Workplace Violence is mostly descriptive in nature. Conclusion: Research is needed to address the dynamics of this global problem. Research supported by: USF ERC and CDC/NIOSH

Abstract #: A-90 Presented by: Mary Hadsell, MS, Graduate Student Examining the Differences in Work-Related Stress, Quality of Life Mary Hadsell RN, MSN;Cecile Lengacher, Ph.D USF CON Keywords: work-related stress, job satisfaction, anticipated turnover, quality of life, clinical nurse leader Objective: The purpose of this study is to examine the difference the Clinical Nurse Leader role has on work related stress, quality of life, job satisfaction and turnover of acute care nurses. In addition, this research will examine the inter-relationships between work related stress, quality of life, job satisfaction, and anticipated turnover. Methods: An ex post facto design will be used to test the hypotheses of this study, which was designed to examine the differences of the newly created Clinical Nurse Leader (CNL) role with work-related stress, quality of life, job satisfaction, and anticipated turnover of acute care nurses. In addition, the study will examine the interrelationships among work-related stress, quality of life, job satisfaction, and anticipated turnover. RNs will complete five survey instruments, including a researcher-developed demographic form. The other four surveys will measure the following variables: work-related stress, job satisfaction, quality of life, and anticipated turnover. All eligible participants will complete an informed consent form. Each participant’s data records will be assigned a unique study number that does not contain any personal identifying information. Results: THIS IS A DISSERTATION PROPOSAL - THERE ARE NO RESULTS AS YET Conclusion: Conceptual Framework Research on work related stress factors, job satisfaction and overall perception of health in nursing show a relationship to retention of nurses in the profession. Improving these factors through implementation of the Clinical Nurse Leader role could result in a decrease in work related stress, an increase in overall perception of quality of life and improved job satisfaction, thereby decreasing attrition from the profession.

Abstract #: A-91 Presented by: Nicole Jagusztyn, MA, Graduate Student Racial Identity: Implications For Workplace Discrimination Nicole Jagusztyn, MA, USF, College of Arts and Sciences, Department of Psychology Keywords: discrimination, racial identity, coping, job stress Objective: The purpose of the current study was to investigate the relationships among perceived workplace discrimination, racial identity, and coping strategies used by black employees. While perceptions of discrimination and use of coping strategies have been widely researched, few studies have focused on the work environment or considered racial identity. Methods: Seventy-three employed, black individuals (81% female) took a web-based questionnaire. Average age of the sample was 29 years (SD=10.07). The web-based survey consisted of three measures: frequency/stressfulness of workplace discrimination, racial identity, and use of maladaptive coping strategies including denial, behavioral disengagement, acceptance, and alcohol/drug use. Results: The measures were used to test a SEM model where workplace discrimination has a direct effect on coping strategies, and racial identity impacts both perceived discrimination and strategy use. Fit indices including a non-significant chi square (p=.08), Bentler’s comparative fit index (0.95), and an acceptable RMSEA estimate (.08) suggest the model has good fit. Conclusion: The model replicates previous findings where perceived discrimination impacts the use of coping strategies. Moreover, results indicate that racial identity affects the perception of discrimination in addition to maladaptive coping. Specifically, as racial identity increases, perceptions of workplace discrimination increase but use of maladaptive coping strategies decreases. Thus individuals with a stronger racial identity may perceive more discrimination, but they avoid dysfunctional ways of dealing with this stressor. Research supported by: This research was supported in part by a NIOSH training grant (No. T42-CCT412874) from the Sunshine ERC at the USF.

Abstract #: A-92 Presented by: Jennifer M. Logan, BA, Graduate Student Effects of Heat Stress on Risk Perceptions and Risk Taking Jennifer M. Logan, Chu-Hsiang Chang, PhD Department of Environmental & Occupational Health COPH USF Keywords: Heat stress, Risk Objective: The purpose of the study was to examine the effects of heat stress on risk perceptions and risk-taking behavior. Methods: Data were collected from 6 healthy male participants between ages 20 and 40, medically prescreened for physical fitness. Participants were exposed to heat stress at 50 degrees Celsius and 20% relative humidity for 2 hours a day, 5 consecutive days. Their perceptions of risk associated with safety activities (Risk Attitude Scale; Weber et al. 2002) and their risk-taking behavior (Balloon Analogue Risk Task [BART]; Lejuez et al., 2003) were assessed at three times: Baseline (prior to any exposure to heat stress), post initial exposure (one-hour after exposure on day 1), and post acclimatized exposure (one-hour after exposure on day 5). Results: In comparison with the baseline (M=3.92), participants perceived the same risky safety-related behaviors (e.g., not wearing a helmet when riding a bike) to be significantly less risky after their initial exposure to heat stress (M=3.57; Z=-1.90, p<.05), but not after their acclimatized exposure (M=4.00; Z=-.18). Similarly, comparing with the baseline (M=40.74), they showed more risk-taking behavior during the BART after initial exposure (M=42.21; F(1,4)=11.39, p<.05), but not after acclimatized exposure (M=41.47; F(1,4)=3.70). Conclusion: This study showed that initial exposure to heat stress shifts individuals’ risk judgment to view the same behavior as less risky. Consequently, they act in a more careless manner. However, acclimation training may help them adapt, thereby allowing them to make cognitive and behavioral adjustments to avoid negative outcomes, such as accidents and injuries. Research supported by: Sunshine Education and Research Center (ERC)

Abstract #: A-93 Presented by: Adam Marty, MSPH, Graduate Student Ethyl Methacrylate and Methyl Methacrylate Exposure of Fingernail Sculptors Adam Marty, MSPH Steven P. Mlynarek, Ph.D.USF COPH, Department of Environmental & Occupational Health Keywords: acrylic nails, artificial nails, cosmetologists Objective: The objectives of this study were to measure nail sculptors’ exposure to ethyl methacrylate (EMA) and methyl methacrylate (MMA) vapors in their personal breathing zone, describe the interior lay-out of the nail salon in relation to where the chemical vapors were generated, and quantify the volume of air supplied by the air handling unit. This study was designed to further characterize and quantify nail technicians’ exposures to EMA and MMA. Methods: Two nail salons were identified as study sites. Nail sculptors’ personal breathing zones were sampled for the duration of a work shift using active sampling media. Nine samples were collected resulting in five 8-hour time weighted averages (TWA). Samples were analyzed by gas chromatography with dual flame ionization detection, per a modified OSHA 7 Method. The ventilation system was assessed using an Alnor Balometer. Results: The 8-hour TWAs averaged 15.3 ppm EMA at one site and 5.2 ppm MMA at the other site. Analysis of a sample of the vapors from the liquid acrylic bottles supplied by the salons yielded a concentration of 5300 ppm of EMA at the first salon compared to 5400 ppm of MMA at the second salon. The percentage of time spent working with the liquid acrylic varied from 20 % to 35 % of the total time of the procedure, regardless if it was a full set or a fill-in. Conclusion: The exposure levels encountered in this study were similar to those reported in the literature. These levels were below any United States occupational exposure limits in place or recommended. It appeared that the time spent working with the liquid acrylic was fairly consistent and therefore predictable. The results of this study suggested that MMA was used at one salon despite a ban on its use in nail products. Research supported by: Sunshine Education and Research Center (ERC)

Abstract #: A-94 Presented by: Sheila Mohammed, MSPH, Resident Cost-effectiveness of Epidural Steroid Injection to Treat Back Pain Sheila Mohammed, MSPH Karen Olson, MD - Occ. Med. Residency, Stuart Brooks, MD - Occ. Med. Residency USF COPH, Department of Environmental & Occupational Health Keywords: Epidural, back pain, cost-effectiveness, narcotics Objective: To determine if Epidural Steroid Injecions (ESI) will result in reduction of pain levels and medication used, and to determine the cost of treatment. Methods: A randomized list of 600 charts was reviewed and 100 selected. Pain scores, medication groups, and narcotics data were abstracted before and after the procedure. Results: Mean pain score before was 7.2 and 7.6 after ESI. Mean pain medication groups before was 2.4 and 3.03 after ESI. Mean narcotic dose before was 11.8 and 23.1 after ESI. Conclusion: ESI to treat back pain does not reduce pain levels, pain medications, or narcotic doses. These measures of discomfort remained the same or were increased. Research supported by: Sunshine Education and Research Center (ERC)

Abstract #: A-95 Presented by: Kristen Shockley, MA, Graduate Student Understanding Motives for Flexible Work Arrangement Utilization Kristen Shockley, MA, Tammy D. Allen, USF.College of Arts and Sciences, Department of Psychology Keywords: flexible work arrangements, flextime, flexplace, motives, family-friendly benefits Objective: The current study addresses a gap in the literature involving family-friendly benefit utilization by examining employees’ motives behind flexible work arrangement (FWA) use. Specifically, we compare the relationships of gender, marital status, family responsibility, and tenure status to life management and work-related motives. Methods: A survey containing measures of gender, marital status, tenure status, family responsibility and motives for flextime and flexplace use (e.g., I alter the timing of my work for childcare purposes, I alter the location of my work for productivity purposes was administered to 148 faculty members from a large southeastern university. Results: For both flextime and flexplace, work-related reasons (productivity and to garner ideas/inspiration) were the strongest motives. Reduction in commute time was the next most popular, followed by life management reasons (childcare, work-family balance, personal reasons). For both FWA, pressure from coworkers was least motivating. There were no gender differences in life management motives; however, women were more likely to use flexplace for work-related reasons than were men. Married individuals and those with greater family responsibility were more likely to use FWA for life management motives. There were no motivation differences for those who were tenured vs. those who were not. Conclusion: The pattern of results suggest that individuals may be using FWA primarily for work-related reasons, namely to enhance their efficiency and productivity. Theses findings provide preliminary evidence that FWA may help the bottom line, a notion that may increase organizations interest and actual support of such policies. Research supported by: In part by NIOSH training grant (No. T42-CCT412874)

Abstract #: A-96 Presented by: Liu-Qin Yang, MA, Graduate Student What Exactly Is Uncivil In The Workplace: A Qualitative Study With A Chinese Sample? Liu-Qin Yang, Paul E. Spector, USF, COM, Xichao Zhang Xuanhui Lin,Beijing Normal University USF, College of Arts and Sciences, Department of Psychology Keywords: workplace incivility, China, source of incivility, uncivil behavior, qualitative approach Objective: This study aims to explore potential categories of incivility behaviors and sources of incivility in a group of Chinese company employees. We also attempt to examine the gender difference in reporting incivility behaviors and sources of incivility. Methods: Incivility incidents at work were collected by asking the participants an open-ended question. Two of the authors independently coded the 596 responses from 596 full-time employees (30% male with average age of 25 years old) who worked for a large mobile company in Beijing, China. The initial inter-rater reliability was .87 for source of incivility, and was .92 for uncivil behaviors. All the discrepancies were resolved after discussion. Results: We found that all the incivility behaviors can be grouped into 12 categories: Nasty verbalization, rude/impolite, annoy you, blame you, sexual harassment, inappropriate physical behaviors, fail to support your efforts, ignore your opinions, fail to empathize you, ridicule, treat you unfairly, and challenge your authority, in the order of frequency reported. Six sources of incivility were reported: Supervisor, coworker, customer, subordinate, others, and observed incivility. Further, the results showed that the females reported more experiences of nasty verbalization, being treated rude/impolite, and sexual harassment, while the males reported more incidents of being blamed, inadequate support, experiencing inappropriate physical behavior, and opinions being ignored. However, there was no significant gender effect on reporting the sources of incivility. Conclusion: Our study contributes to the literature of workplace incivility by making one of the first empirical and qualitative endeavors in China. Research supported by: http://www.eap.com.cn/

Abstract #: A-97 Presented by: Sam Al-Saadi, MD, Resident Repeat Attempt at Endoscopic Polypectomy Intraoperatively Rather than Surgery Avoids the Need for Colorectal Resections for Benign Tumors Sam Al-Saadi, MD, Sowsan Rasheid MD (USF), Jose Lopez MD (USF), Jorge Marcet MD (USF) USF COM, Department of Surgery Keywords: Colonoscopy, Polyps, Laparoscopic-assisted Objective: Patients with colorectal adenomas that do not undergo complete polypectomies are referred for surgical resection. Many of these adenomas are considered too large or are found in difficult locations that prevent complete removal by the referring gastroenterologists. This study is undertaken to evaluate the validity of attempted redo intraoperative polypectomies before surgical resection. Methods: From 2001 to 2006, 76 patients with nonmalignant colorectal adenomas were referred for surgical resection. The patients underwent a repeat colonoscopy with polypectomy in the operating room (OR). Laparoscopic-assisted endoscopic polypectomy was undertaken as necessary. Finally, bowel resection was done when endoscopic resection was insufficient to remove the adenoma. Utilizing a chi-square analysis and Mann-Whitney U-test, hospital length of stay, adverse events, and recurrence rate were compared for patients with redo polypectomy with or without laparoscopic assistance, and with bowel resections. Results: 76 patients (58% men) underwent redo polypectomy and were followed a median of 12 months. 53 patients had complete endoscopic polypectomy, of which 5 required laparoscopic-assistance, and 23 required bowel resection. Median hospital length of stay for patients after endoscopic polypectomy alone was 0 days vs. 1 day after laparoscopic assistance (p=0.05) and 3 days after bowel resection (p<0.0001). Conclusion: Intraoperative colonoscopy allows for more aggressive approaches at polypectomy since complications can be dealt with immediately. Patients that undergo a complete redo polypectomy have shorter length of stay with no difference in recurrence compared to patients that undergo bowel resection.

Abstract#: A-98 Presenter: Jan Boger, Au.D., Faculty Identification of Vestibular Dysfunction Using the Sinusoidal Harmonic Acceleration (SHA) Test and the Step Velocity Test (SVT) Jan Boger, Au.D., Asst Prof., Dir. of Audiology- USF Hearing & Balance Center, Dept. of Otolaryngology-HNS, COM K. Paul Boyev. M.D., Asst Prof., Dept. of Otolaryngology-HNS, COM, Raymond M. Hurley, Ph. D., Assoc. Prof.,Theresa Chisolm, Ph.D., Prof. & Chair, Dept. of Communications, Sciences, & Disorders, CAS Key Words: Vestibular Dysfunction, Vestibular Ocular Reflex, Sinusoidal Harmonic Acceleration Test Step Velocity Test Objectives: The purpose of this investigation was to determine if the Sinusoidal Harmonic Acceleration Test (SHA) and the Step Velocity Test (SVT) are equivalent identifiers of vestibular dysfunction when using the parameters of gain and time constants. Methods: 100 Videonystagmography rotary chair SVT & SHA results were analyzed. Average gain was calculated for the SVT (Gain STEP). SHA test gains per frequency were compared to the Gain STEP. Abnormal gain and phase was determined by the manufacturer’s normative data graph. Gain STEP < 0.40 was indicative of vestibular dysfunction. Averaged time constants of the SVT and SHA (converted phase angles) was compared. Two consecutive data points outside the range of normal and time constants < 13 seconds were suggestive of vestibular dysfunction. Results: There was no significant difference between the SHA and SVT tests in categorizing subjects as normal or abnormal. 61% of the subjects were identified as abnormal by both tests. The SHA test identified gain abnormalities and the SVT identified time constant abnormalities. Conclusion: The SVT and SHA test appear to be equivalent identifiers of vestibular dysfunction. There is no significant difference between the SVT and SHA test in categorizing subjects as normal or abnormal. The SHA test identified primarily gain abnormalities and may be the “stronger” test for identifying overall poor system response. The SVT identified primarily time constant abnormalities and may be a “stronger” test for identifying poor central velocity storage abnormalities. The 2 data point criterion is appropriate. Implications suggest neither test should be omitted, but rather both tests should be used in conjunction to provide the appropriate diagnostic conclusion.

Abstract #: A-99 Presented by: Byers Bowen, MS, Med II Student Calcium and Vitamin D metabolism as Related To Pseudohyperparathyroidism In Morbidly Obese Patients Following Bariatric Surgery. Byers Bowen, MS,USF, COM, Scott F. Gallagher, MD Assistant Professor of Surgery, USF, Michel M. Murr, MD Professor of Surgery, USF, Krista L. Haines, MA USF,COM, Surgery Keywords: Bariatric, Calcium, Vitamin D, Parathyroid hormone Objective: Obesity is an epidemic, and bariatric surgery is the only durable alternative for the treatment of clinically significant obesity. Very little information exists regarding bone health/metabolism and the natural history of serum calcium, alkaline phosphatase, intact PTH (parathyroid hormone) and vitamin D in patients undergoing bariatric surgery. In addition, little is known regarding the pathophysiology of calcium and vitamin D as related to intestinal absorption, renal metabolism, and parathyroid function after bariatric surgery. The purpose of this study was to examine the relationship of calcium, intact PTH (parathyroid hormone), and vitamin D in bariatric patients; ultimately, this study will help to better guide patient management for all bariatric patients Methods: Retrospective chart analysis performed on 1017 patients that underwent bariatric operations from 1998 to November of 2006 at Tampa General Hospital. Serum calcium, parathyroid hormone (PTH), and alkaline phosphatase data were measured 1, 2, 3, and 4+ years post-operatively and plotted using a bar graph. Results: A total of 102 morbidly obese patients with elevated PTH values were analyzed. Yearly post-operative PTH values demonstrated a linear annual increase. Calcium and alkaline phosphatase values remained constant. Conclusion: The linear, annual increase of intact PTH following bariatric surgery is interesting and clinically relevant. This postoperative secondary hyperparathyroidism results from either relative hypocalcemia, vitamin D deficiency or both; further study is needed, particularly as it relates to bone health. Annual Vitamin D and intact PTH should be considered routinely in postoperative bariatric surgical patients. Research supported by: USF Health College of Medicine Office of Research

Abstract #: A-100 Presented by: Ali Bozorg, MD, Resident Vascular Cognitive Syndromes In Stroke Patients: Relation To Stroke Etiology And Topography In 2389 Cases Ali Bozorg, MD, Michael Hoffmann, M.D., Professor of Neurology and Associate Dean, COM, USF, Department of Neurology Keywords: Stroke, cognitive syndromes, TOAST classification Objectives: Cognitive syndromes (CS) after stroke may be important to measure and monitor for management and emerging therapies Methods: A validated cognitive examination was administered during the first month of stroke presentation and analyzed according to 5 networks for cognition and correlated with neuropsychological tests. Multivariate analyses (MVA) were performed to determine association of cognitive syndromes with etiology (TOAST classification) topography and neurological deficit by NIHSS. Results: After specific exclusions, one or more CS was present in 112 of 309 (36%) TIA patients versus 1569/1796 (87%) stroke patients (p=<0.001). The frequency of frontal network syndromes (FNS) was 908/1796 (51%), left hemisphere network (LH) syndromes 646/1796, (36 %), right hemisphere (RH) network syndromes 275/1796 (15.3%), occipitotemporal network (OT) syndromes 107/1796 (6 %), hippocampal limbic (HL) network syndromes 397/1796 (22 %) and miscellaneous (M) syndromes of 481/1796 (27%). Stroke etiology and their signature cognitive syndromes by MVA, revealed significant associations for LH with cardioembolism (OR 1.61, p=0.0029), FNS and “other” etiology (OR 1.96, p=<0.0001) and HL also for “other” etiology (OR 1.57, p=0.0026). A FNS was associated with frontal lesions (OR 5.19, <0.0001) as well as subcortical lesions (OR 1.91, p= <0.0001). The M group of CS were associated with subtentorial (OR 1.86, p=0.0283) and right hemisphere lesions (OR 2.47, p= <0.0001). Conculsion: CS are present in the vast majority of stroke patients. Certain stroke subtypes are associated with specific CS. Certain signature CS result from lesions that relate to the major cognitive networks.

Abstract #: A-101 Presented by: Stephanie Carey, MS, Graduate Student Compensatory Motion While Using A Transradial Prosthesis During Common Tasks Stephanie Carey, MS,,M.Jason Highsmith PT, DPT, CP, FAAOP, University of South Florida,School of Physical Therapy & Rehabilitation Sciences, Rajiv V. Dubey, PhD, University of South Florida, Dept. of Mechanical Engineering USF College of Engineering, Department of Mechanical Engineering Keywords: prosthesis,transradial, kinematics, motion analysis Objective: The objective of this study was to quantify the compensatory motion caused by the limitations of a transradial myoelectric prosthesis (TRMP) lacking a powered wrist component during common tasks. Quantification of compensatory motion could be used to create a mathematical model to be used for fitting and training of a prosthesis. A secondary objective was to determine if bracing intact subjects could simulate the use of a TRMP. Methods: Seven TRMP users and ten non-amputee volunteers performed four common tasks. Bracing was used to simulate the use of a prosthesis by the non-amputee subjects. Range of motion of the glenohumeral joint, elbow and torso were calculated from optical motion analysis data. The motions between the non-braced, braced and transradial prosthesis user groups were statistically compared. Degree of asymmetry between the affected and unaffected arm was computed for the bilateral tasks. Results: TRMP users compensate for lack of wrist and forearm movement differently for each task. Torso side bending occurs while opening a door. For the box lift task, prosthesis users rely more on the sound arm and torso bending. While drinking from a cup, decreasing flexion of glenohumeral joint and increasing elbow flexion occurred while using a prosthesis. Prosthesis users turn a steering wheel similarly to non-amputee subjects. Conclusion: By looking at the compensatory motions caused by limiting forearm and wrist movement, a greater understanding of the problems with transradial prosthetic design can be developed. Although bracing intact subjects showed similar mechanisms of compensation in most tasks, the magnitude of compensation was greater for prosthesis users. Research supported by: U.S. Dept. of Education, Rehab. Services Administration; #6140100000

Abstract #: A-102 Presented by: Jason Guthrie, MD, Resident Developmental Susceptibility of Reactive Arthritis Jason Guthrie, MD,John D. Carter, MD. Assitant Professor, Dept. of Rheumatology USFCOM Keywords: CCR5-delta 32 mutation Chlamydia trachomatis Reactive Arthritis Objective: To see if the CCR5-delta 32 mutation affects disease susceptibility and/or chronicity of Ct-induced ReA. Methods: Study subjects are recruited from the Hillsborough County Department of Health in Tampa, Florida when they present with symptoms of an acute venereal disease and test positive for Ct. Study subjects are contacted by telephone 6 weeks after their acute Ct infection when they are queried for any new arthritis symptoms (or other non-articular symptoms consistent with ReA). Any subject who developed symptoms consistent with ReA are followed with telephone interviews at weeks 12, 26, and 52 to track disease chronicity. All study subjects are tested for the CCR5-delta 32 mutation. Results: A total of 80 subjects have been enrolled. The study population consists of 90 males and 83 females with a mean age of 24.5 years. The ethnicities include 111 African American, 31 Hispanics, 27 Caucasians, and 4 other. 9 of 75 subjects (12%) were identified as having new symptoms consistent with ReA at the 6 week telephone interview. At the 12-week telephone follow-up 8 subjects were contacted (1 loss to follow-up); 3 subjects had resolution of symptoms and the other subject’s symptoms were persistent. At the 26-week follow-up 5 subjects were contacted (4 loss to follow-up) all with resolution of symptoms. At the 52-week follow-up 4 subjects were contacted (5 loss to follow-up) with continued resolution of symptoms. So far, of 75 subjects screened 3 have been positive for the CCR5-delta 32 mutation. Conclusion: The preliminary data suggest that the CCR5-delta 32 mutation does not affect the disease susceptibility of Ct-induced ReA. However, our study population’s prevalence of this mutation appears lower than previous reports.

Abstract #: A-103 Presented by: Bengt Herweg, MD, Faculty Prolonged Left Ventricular Latency May Require Interventricular Interval Adjustments to Assure Response to Cardiac Resynchronization. Bengt Herweg, MD, Rias Ali, MD, Chris Madramootoo, Arzu Ilercil, MD, S. Serge Barold, MD Arrhythmia Service and Division of Cardiology, USF COM, Department of nternal Medicine Keywords: Cardiac resynchronization therapy, Electrocardiography, Device optimization,Ventricular Latency. Objective: Long left ventricular (LV) latency intervals can result in suboptimal cardiac resynchronization therapy (CRT) during simultaneous biventricular pacing. We investigated how differences in LV and right ventricular latency intervals in patients undergoing CRT impact on the hemodynamically optimized VV interval during biventricular pacing. Methods: We acquired high speed 12 lead ECG recordings in 37 patients with CRT devices during left and right ventricular pacing at three different stimulus output settings (threshold, threshold x3, and maximal output). Stimulus-to-earliest QRS deflection (=latency) intervals were measured with digital calipers. Doppler echocardiographic AV and VV optimization was performed using aortic velocity time integrals as a surrogate for left ventricular stroke volume. Results: Latency intervals were longer during LV pacing when compared to RV pacing (p<0.001) and shortened with increased stimulus strength (p<0.05). 5/37 (14%) patients exhibited prolonged LV latency intervals >40 ms. LV Latency intervals and delta latency (defined as LV-RV latency interval) correlated with the optimized VV interval (R=0.45 and 0.36, p<0.01 and p<0.05 respectively. Conclusion: LV pacing from the lateral cardiac vein is associated with longer latency intervals than RV endocardial pacing. LV latency is associated with delayed LV activation and, hence, may require VV interval adjustment to improve hemodynamic response to CRT. Thus, a high speed ECG recorded during LV pacing may identify patients who could benefit from formal VV interval optimization.

Abstract #: A-104 Presented by: M. Jason Highsmith, DPT, CP, FAAOP, Graduate Student Comparison of C-Leg Microprocessor Knee With Non-Microprocessor Knees On Walking Tests, Stair Descent, Questionnaire, Preference, Stumbles And Falls M. Jason Highsmith, Jason T Kahle CPO Westcoast Brace & Limb, USF COM School of Physical Therapy and Rehabilitative Sciences Keywords: Amputee, Falls, Prosthetic Knee, Transfemoral, Walking Objective: To compare transfemoral amputees function in objective and subjective evaluative measures between their previously accommodated non-microprocessor prosthetic knee with function while using a C-Leg microprocessor knee. Methods: 19/21 subjects completed the following evaluative measures after a 90 day accommodation period in a non-microprocessor prosthetic knee: subjective questionnaire, reporting the number of stumbles and falls in the previous 90 days, a stair descent test, and 4 walking tests of varied distances, terrain and time constraints. Subjects were issued a C-Leg prosthetic knee and allowed to accommodate and return for follow up as needed prior to re-testing. Subjects re-tested following 90 day accommodation with the C-Leg. Results: Use of a C-leg improved function in nine out of nine outcomes: 1. PEQ scores increased 20% (p=0.007). 2. Stumbles decreased 59% (p=0.006). 3. Falls decreased 64% (p=0.032). 4. SSWS (75m) improved 15% (p=0.030). 5. FPWS (75m) improved 12% (p=0.005). 6. FPWS (38m) on uneven ground improved 21% (p<0.0001). 7. FPWS (6m) improved 17% (p=0.001). 8. Twelve subjects increased their MRPP PCS scores in stair descent. 9. Fourteen subjects preferred the C-Leg as opposed to their previous NMKM. Conclusion: Objective evaluative clinical measures are vital to justifying medical necessity of knee mechanisms in transfemoral amputees. Use of the C-leg improves performance, quality of life, and can increase Medicare functional classification level and the level of community ambulation. Research supported by: Demonstration project on Prosthetics and Orthotics. Department of Education, Rehabilitation Services Administration. Award #H235J050020

Abstract #: A-105 Presented by: Sandra Jara, MD, Resident Treatment Outcomes in Thrombocytopenic Patients Undergoing Therapy with Interferon and Ribavirin for Chronic Hepatitis C Sandra Jara, MD, Eknath Naik, MD, PhD; William Boyd, MD USF COM, Department of Internal Medicine Keywords: Hepatitis C Thrombocytopenia Cirrhosis Treatment Objective: Analyze final treatment outcomes in Hepatitis C patients with initial platelet counts < 100,000 who undergo treatment with Interferon and Ribavirin. Methods: A retrospective follow-up study was performed using the James A. Haley VA Liver Clinic database in patients who have undergone combination treatment with interferon and ribavirin, from 1/1/2001 to 6/30/2004. Patients were identified before treatment initiation and were grouped into two categories based upon platelet counts below 100,000 (study group) vs more than and equal to 100,000 (control group), and their response to treatment in terms of dose reduction, virologic response, and blood counts were followed. The statistical analysis was performed using SPSS 15.0 statistical package. Both Chi-square and student-t test were used to test for statistical significance based upon type of variable. Results: 560 patients were included in the study, of which 63 were in the study group and 497 in control group. Overall, there were 556 males and only 4 were females. The mean age in study and control group was comparable ( P > 0.05). When stratified by genotype, it was found that those with genotype 1, 16.7% had SVR in study group compared to 47.2% in control group while in genotype 2 , 37.5% had SVR in study group compared to 47.8% in control group, whereas in genotype 3, 66.7% had SVR in study group compared to 65.6% in control group. Conclusion: SVR is more difficult to achieve in cirrhotic patients and is highly dependent on HCV genotype. HCV genotype should be a determinant in the decision pathway to initiate combination therapy with Pegylated Interferon and Ribavirin in the cirrhotic patient.

Abstract #: A-106 Presented by: Jennifer Logan, MD, Faculty The Changing Profile of HIV/AIDS Patients in Hillsborough County Jennifer Logan, MD, Lynette Menezes, PhD Department of Internal Medicine, Yiliang Zhu, PhD, COPH Department of Epidemiology and Biostatistics), Karina D'Souza, MPH (Staff, College of Medicine, Department of Internal Medicine, Yangxin Huang, PhD, COPH Department of Epidemiology and Biostatistics, Hyungtae Kim, MS, COPH Department of Epidemiology and Biostatistics, Gloria Santayana, MS COM, Department of Internal Medicine Keywords: HIV, AIDS, demographic, Hillsborough, Florida Objective: This study aims to describe the demographic profile for HIV-infected persons utilizing Health Department services in Hillsborough County, Florida. Methods: The study sample included 2303 HIV-infected persons who established care at the Hillsborough County Health Department between January 1, 2000 and December 31, 2006. Existing data elements were extracted from the Health Department’s database, and secondary data analysis was performed using SAS and S-PLUS. Results: Subjects’ mean age was 39.7, including 66.7% males, and 69% ethnic minorities. Most subjects (98.4%) reported public assistance or no insurance at enrolment. Ryan White Program utilization increased from 15.2% to 52.8% over the study period (p < .0001). African Americans and Hispanics were older at enrollment compared to Whites (p = .0449). Subject ethnic composition changed over the study period (p = .0055); in particular, Hispanic enrolment increased significantly. HIV risk behavior varied by enrollment age (p < .0001): young age was associated with men having sex with men; increasing age was associated with heterosexual contact; injection drug use was associated with middle age. Risk behaviors varied by ethnicity (p < .0001), with African Americans (74.5%) and Hispanics (58.4%) more likely to report heterosexual contact versus Whites (32.2%). Conclusion: HIV-infected persons utilizing local health department services include predominantly minorities of lower socioeconomic status who receive public assistance for HIV/AIDS care. Disparities exist with respect to ethnicity and demographic factors such as age at enrolment, HIV risk behavior, and insurance coverage. These findings underscore the need for further studies and interventions targeted to local minority populations.

Abstract #: A-107 Presented by: Candice Mateja, D.O., Resident Myocardial Ischemia Due to Cardiac Tamponade Candice Mateja, D.O.,Malika George, MSIV, Joseph Mishkin, MD, Maya Guglin, MD USF COM, Cardiology Keywords: cardiac tamponade myocardial ischemia Case report A 47 year old man with a history of hypertension, diabetes mellitus, and obesity presented with a 3 day history of chest pain, shortness of breath, orthopnea, and lower extremity edema. The patient denied previous history of chest pain. He was markedly hypertensive on presentation. Physical exam was significant for a grade 2/6 early systolic ejection murmur, bilateral pulmonary rales and expiratory wheezes. He also had 2+ pitting edema of his lower extremities. ECG showed nonspecific ST changes. After stabilization of his blood pressure, an adenosine thallium stress test was ordered to rule out ischemic heart disease. On stress images, moderate areas of decreased perfusion consistent with ischemia were noted in the lateral and inferolateral walls. Subsequent echocardiogram demonstrated a large pericardial effusion with tamponade physiology. An emergent pericardial window operation was performed, with immediate resolution of all symptoms. A repeat nuclear stress test after removal of pericardial fluid showed normal perfusion. Two months later the patient was admitted for another reason and coronary angiography was performed. It revealed normal coronary arteries. Possible interpretations of initial abnormal stress test include transient ischemia caused by cardiac tamponade in the absence of coronary artery disease, or a false positive stress test due to an artifact created by pericardial effusion. To our knowledge there has been no previous case report in the literature of documented myocardial ischemia secondary to a large pericardial effusion.

Abstract #: A-108 Presented by: Sameer Nagamia, MD, Resident The Effects of Extended-Release Niacin on Carotid Intimal Media Thickness, Endothelial Function and Inflammatory Markers in Patients with the Metabolic Syndrome Sameer Nagamia, USF COM.and Emory University; Martin Thoenes, University of Dresden; Asahiko Oguchi, Christopher Vaccari, Emory University; Rammadan Hammoud, Emory University; Guillermo Umpierrez, Emory University; Bobby Khan, Emory University, UAF COM, Department of Anesthesiology Keywords: Extended release niacin; Metabolic Syndrome Objective: Niacin is an agent that significantly increases high-density lipoprotein cholesterol (HDL-C), but its effects on surrogate markers of atherosclerosis and inflammatory markers are less clear. We studied the effects of niacin on carotid intimal media thickness (IMT), brachial artery reactivity as well as markers of inflammation and the metabolic profile of patients with metabolic syndrome(MetSyn). Methods: Fifty patients with MetSyn (Adult Treatment Panel III criteria) were randomized to either ER niacin (1000 mg/day) or placebo. We studied the effects of niacin on carotid intimal media thickness (IMT), brachial artery reactivity as well as markers of inflammation and the metabolic profile of patients with MetSyn. Results: Patients were randomized to either ER niacin (1000 mg/day) or placebo. After 52 weeks of treatment, there was a change of carotid IMT of +0.009 +/- 0.003 mm in the placebo group and -0.005 +/- 0.002 mm in the niacin group (p = 0.021 between groups). Endothelial function improved by 22% in the group treated with niacin (p < 0.001), whereas no significant changes were seen in the placebo group. High sensitivity C-reactive protein decreased by 20% in the group treated with niacin for 52 weeks (p = 0.013). Niacin increased HDL-C (p < 0.001) and decreased low-density lipoprotein cholesterol and triglycerides (p < 0.001) significantly, and there were no adverse effects on fasting glucose levels after 52 weeks of treatment. Conclusion: ER niacin therapy effects a regression in CIMT and improvement in metabolic parameters (increased HDL and reduced triglycerides). Furthermore, ER niacin may demonstrate an anti-atherogenic effect in the metabolic syndrome by improving endothelial function and decreasing vascular inflammation.

Abstract #: A-109 Presented by: Brian Palumbo, MD, Resident Distal Femoral Reconstruction for the Treatment of Malignant Bone Tumors Brian Palumbo, M,D, Richard Cain, M.D.1, Nazeem Virani, M.D.1, Eric Henderson, M.D.1, German A. Marulanda, M.D.1, and G. Douglas Letson, M.D.2 USF COM, Department of Orthopaedics and Sports Medicine Keywords: "distal femur" reconstruction, osteosarcoma, tumor Objective: The long term results of endoprostheses for distal femoral reconstructions have been found to be suboptimal by several investigators. The purpose of this study is to describe a technique of distal femoral reconstruction using an endoprosthesis and to evaluate clinical and radiographic results with a medium to long term follow up. Methods: From February, 2000, to October, 2006, 58 patients with skeletal malignancies of the distal femur were treated with distal femoral reconstructions using an endoprosthesis. All procedures were performed by the senior author following the same surgical technique. All patients were evaluated clinically and radiographically for a mean of fifty months. Patients were evaluated post operatively for rates of complications, range of motion, level of pain, ability to ambulate without assistance, and radiographic appearance. Results: Forty-one patients were diagnosed with a primary bone tumor of the distal femur and seventeen patients had a diagnosis of metastatic disease.The mean post operative range of motion was ninety-eight degrees. There were four cases of wound dehiscence and four cases of infected surgical sites that required debridement and irrigation. There were only two episodes of mechanical failure of the endoprosthesis. Two patients presented with radiographic loosening of the prostheses that required surgical revision. Conclusion: The authors believe that prosthetic reconstruction of the distal femur is a viable option that should be considered in all patients with malignant tumors that meet the criteria for limb salvaging techniques.

Abstract #: A-110 Presented by: Joshua Phillips, MD, Resident Effect of Supplemental Oral Vitamin E in Patients with Elevated Serum IgE and a History of Atopic Conditions Joshua Phillips, MD, Gailen Marshall, MD Division of Clinical Immunology, University of Mississippi, Jackson, MS University of South Florida College of Medicine Internal Medicine Keywords: Vitamin E Antioxidant Regulatory T Cells Tregs IgE Objective: Oral vitamin E has been shown to decrease serum IgE level in patients with atopic dermatitis. This may occur via increased activity of regulatory T cells (CD4+CD25+Foxp3). The effect of vitamin E in other atopic conditions has not been studied.

Methods: A randomized, double-blinded, placebo-controlled trial was designed to investigate the effect of oral vitamin E supplementation in adolescents and adults with elevated serum IgE (>150IU/mL) and a current history of atopic/allergic conditions. Participants were randomized to receive oral Vitamin E gelcaps 400IU/day, 1000IU/day, or placebo. Participants underwent blood sampling on day 0, day 14, and 28 for serum IgE, intracellular cytokine profiling, and CD4+CD25+Foxp3 cells, expressed as percentage of CD4+ cells. Results: A total of thirty-three (33) participants were recruited, with a mean age 32.3 years and with mean IgE 360.6IU/mL. 90.9% carried a diagnosis by physician of allergic rhinitis, 18.2% were diagnosed with atopic dermatitis, 9.1% asthma, 6.1% other. Nineteen participants were found to have an elevated serum IgE level, mean 488.0IU/mL; three participants withdrew prior to completion of the study. There were no statistically significant changes in total serum IgE with oral Vitamin E gelcaps at 400IU or 1000IU. Further, there were no statistically significant increases in percentage of CD4+CD25+Foxp3 cells expressed as a percentage of CD4+ cells.

Conclusion: Vitamin E did not significantly alter serum IgE or significantly affect absolute or percentage CD4+CD25+Foxp3 cells. More research is needed to discover the possible relationship of vitamin E or other antioxidant therapy on atopic conditions.

Abstract #: A-111 Presented by: Gilbert Postler, MD, Faculty High Dose Nitroglycerin Reverses Refractory Cardiac Arrest Gilbert Postler, MD Maya Guglin, MD Assistant Professor of Cardiology, USF COM Keywords: High Dose Nitroglycerin Cardiac Arrest Results: 86-year-old man with a known history of coronary artery disease (coronary artery bypass surgery in 1999 with three bypasses), hyperlipidemia, hypertension, carotid artery disease, and diabetes mellitus type II who cardiac arrested at the out-patient laboratory of the VA Hospital. His initial electrocardiogram rhythm was ventricular tachycardia and his clinical arrest lasted for 60 minutes and ACLS protocol was followed. He was then transferred to the MICU where he arrested again and ACLS protocol was followed for an additional 50 minutes. Prior to aborting the code, as a last resort, 4 mg of NTG were administered intravenously as a bolus. After three minutes, the patient was breathing, in normal sinus rhythm, with a palpable pulse and acceptable blood pressure. The patient was then subsequently supported, and made a full recovery. Conclusion: We have presented a case of dramatic beneficial effects of high dose NTG treatment in cardiac arrest and cardiogenic shock in patients with acute left ventricular failure with unobtainable BP. Numerous case studies and case series exist in the literature detailing the beneficial effects of NTG in this setting. We are proposing to further investigate this subgroup of patients who present with cardiac arrest with presumed acute coronary syndrome. The aim of our research project is to determine if high-dose nitroglycerin can prevent the patient’s death in acute cardiac arrest once standard ACLS protocol has run its course and the treating physician contemplates aborting resuscitation efforts. Research supported by: USF Department of Cardiology

Abstract #: A-112 Presented by: Ronald Purcell, MD, Resident Rush Sublingual Immunotherapy (RSLIT) to Treat Seminal Plasma (SP) Hypersensitivity Ronald Purcell, MD, Dennis Ledford, MD, USF COM, James A. Haley Veterans’ Administration Hospital, Tampa, USF COM, Department of Anesthesiology Keywords: Immunotherapy, SLIT, seminal plasma hypersensitivity Objective: Seminal plasma hypersensitivity is uncommon. Protocols for RSLIT for SP hypersensitivity are not reported. We present an RSLIT protocol to treat seminal plasma allergy. Methods: An RSLIT protocol was utilized for a 25 y/o female with a history of vaginal pruritus, erythema, abdominal pain, pharyngeal and hand tingling as well as a generalized flushing sensation occurring immediately after unprotected intercourse. Symptoms do not occur with condom use. Semen samples were collected from the subject’s husband at a local fertility clinic. Spermatozoa were removed and the SP stored at -40? C. Skin prick test to undiluted SP was negative. 2 ml of 1:10,000 SP with 2 ml of glycerin was initially placed under her tongue for 2 minutes and swallowed. The concentration was increased tenfold every 30 minutes until 2 ml of undiluted SP was administered. Two hours after RSLIT she was released from the clinic with an epinephrine autoinjector and advised to have unprotected intercourse. Results: The subject experienced mild pharyngeal and hand tingling at the beginning of RSLIT that resolved with subsequent doses. These same symptoms reoccurred at the undiluted concentration and resolved after a second undiluted dose. Unprotected intercourse the following day resulted in no symptoms. She remains symptom-free as long as she has unprotected intercourse at least every 96 hours. However, she develops vaginal pruritus, irritation and abdominal pain with longer periods of abstinence that resolve with more frequent intercourse. Conclusion: RSLIT may be a safe and effective treatment for seminal plasma hypersensitivity.

Abstract #: A-113 Presented by: Steven Rakita, MD, Faculty Practical Considerations and Survival Techniques for a Rat Model of Roux-en-Y Gastric Bypass Steven Rakita,Drew A Rideout, Yanhua Peng, William R Gower, Michel M Murr. USF COM Department of Surgery and Research, James A Haley Veterans Affairs Medical Center, Tampa Keywords: Obesity, Gastric bypass, Rodent models Objective: A rat model for Roux-en-Y gastric bypass (RYGB) is associated with high operative mortality. We describe techniques to improve survival of obese rats undergoing RYGB. Methods: Obese Sprague-Dawley male rats underwent RYGB or sham operation. Despite following published protocols we continued to observe high rates of mortality in the immediate post-operative period. We, therefore, introduced a group of interventions aimed at improving survival after RYGB: 1) changed to inhalational anesthetic with streamlined operations to minimize anesthesia exposure; 2) used metabolic cages peri-operatively to prevent bedding and feces ingestion; 3) modified the peri-operative diet with a 12 hour pre-operative fast and a gradual advance to solid chow over 1 week; 4) created smaller gastric pouch and larger gastrojejunostomy to facilitate gastric emptying; 5) minimized manipulation of tissues. Weight and survival in the all groups were compared. Results: RYGB was undertaken in 18 rats according to published protocols and in 21 rats after introducing new interventions. All deaths in the pre-intervention group were within 36 hours of surgery. Overall survival at 6 weeks post-operatively increased dramatically from 11% to 86% immediately after introducing our interventions. Obese rats undergoing sham operations (n=8) had similar overall survival (88%) as post-intervention RYGB rats. Final body weight was lower in RYGB rats compared to sham controls (364±45g vs. 496±32g; p<0.01). Conclusion: RYGB is a technically challenging procedure in rodents. Simple modifications in operative conduct and peri-operative care improve mortality predictably. Lessons learned from our experience should shorten the learning curve for researchers establishing animal models of obesity and RYGB.

Abstract #: A-114 Presented by: Sowsan Rasheid, MD, Resident Radical Resection of Rectal Cancers with Complete Pathological Response To Neoadjuvant Chemoradiation Does Not Improve Outcome Compared to Local Excision Sowsan Rasheid, MD, Sam Al-Saadi MD (USF), Jose Lopez MD (USF), Jorge Marcet MD (USF) USF COM, Department of Surgery Keywords: Rectal Cancer, Neoadjuvant Therapy Objective: The standard treatment for advanced rectal adenocarcinoma consists of neoadjuvant chemoradiation (NeoCh) followed by low anterior resection (LAR) or abdominoperitoneal resection (APR). NeoCh often results in downstaging tumors, and may eradicate the tumor entirely. This study is undertaken to examine the outcomes in patients who had radical resection vs. transanal excision (TAE) after complete pathologic response to NeoCh for rectal adenocarcinoma. Methods: From 1995 to 2005, 235 patients with rectal adenocarcinoma underwent NeoCh followed by surgery. 151 patients had a radical resection, 53 had TAE, 9 had TAE followed by radical resection, and 22 were not resected. Complete pathologic response to NeoCh was defined as T0 or Tis. Patients were followed for complications and recurrence. Adverse events, survival, and recurrence rate were compared for patients that had complete pathologic response. Data are presented as median, mean ± SD. Results: 31 patients (65% men) with median age 67 years were found to have complete pathologic response. 26 patients underwent a TAE, 4 underwent an LAR, and 1 underwent an APR. Disease-free survival for TAE was 91 months, 79 ± 35.2 vs. 34 months, 36 ± 19.4 for radical resection (p=NS, Log-rank test). Postoperative complications were more common amongst patients with radical resection (p=0.0008, Fisher’s exact test). Conclusion: Only 14% of patients that received NeoCh for rectal cancer had a complete pathologic response. Radical resection did not improve outcome but did increase postoperative morbidity over TAE for patients that have complete pathologic response to NeoCh for rectal cancer. This study supports the use of TAE as the sole surgical treatment when complete pathologic response is demonstrated.

Abstract #: A-115 Presented by: Drew Rideout, MD, Resident Gastric Bypass Improves Obesity-Related Steatosis and is Associated with Reduction in Serum Adiponectin Drew Rideout,Yanhua Peng, Steven S Rakita, William R Gower, Min You, Michel M Murr. 1. Department of Surgery and Research, James A Haley Veterans Affairs Medical Center, USF 2. Department of Molecular Pharmacology & Physiology, USF COM, Department of Surgery Keywords: Obesity, Non-Alcoholic Fatty Liver Disease, Gastric Bypass, Adiponectin Objective: Non-Alcoholic Fatty Liver Disease (NAFLD) is a manifestation of obesity and leads to cirrhosis. Adiponectin has direct effects on inflammation and lipid metabolism. We hypothesize that in obese rats, Roux-en-Y gastric bypass (RYGB) improves liver steatosis and induces changes in serum adiponectin. Methods: Sprague-Dawley male rats were fed high fat diet (HFD) to induce obesity prior to RYGB (n=4) or sham (n=3). Rats were sacrificed at 9 weeks post-operatively. Serial body weight and serum adiponectin were measured. Liver sections were stained for fat content. Gels were quantified using densitometry; p<0.05 was significant for T-test. Results: HFD induces obesity and increases body weight in rats (p<0.01 vs. regular chow). RYGB induces sustained weight loss in obese rats (p<0.01 vs. sham). Prior to RYGB, obese rats had increased serum adiponectin compared to regular chow (p<0.01). RYGB reduced serum adiponectin in obese rats compared to obese sham controls (p<0.05); serum adiponectin was also reduced after RYGB as compared to respective pre-operative levels (p<0.01). HFD increased the number and size of fat droplets in the liver compared to regular chow. RYGB decreased liver weight by 30% and significantly reduced fat droplets in the liver compared to obese sham rats. More importantly, the degree of steatosis improved in rats after RYGB as compared to the same rats pre-operatively. Conclusion: HFD induces obesity and steatosis in obese rats. Surgically-induced weight loss in a rat model of RYGB exhibits an anti-lipogenic profile by improving liver steatosis. The novel observation that RYGB reduces serum adiponectin warrants further investigation into the complex signaling between peripheral adipose tissue and the liver in obese rats with NAFLD.

Abstract #: A-116 Presented by: Elizabeth Sagatys, MD, Resident Analysis of T-cell Subpopulations in the Lymph Nodes of Patients with Mantle Cell Lymphoma Elizabeth Sagatys, MD1*, Lynn Moscinski, MD1,2, Sophie Dessureault, MD,PhD2, Eduardo Sotomayor, MD2 and Hernani Cualing, MD1,2. 1Pathology, USSF COM and 2Interdisciplinary Oncology, H. Lee Moffitt Cancer Center, Tampa, FL, USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Mantle cell lymphoma, Regulatory T-cells Objective: Mantle cell lymphoma (MCL) is an aggressive and incurable B-cell NHL characterized by a high relapse rate. To effectively harness the immune system against MCL, it is important to understand how T-cells interact with malignant B-cells. A recently recognized subset of T cells, regulatory T cells (Tregs), are believed to play an important role in autoimmune regulation and suppression of tumor specific immune response. In the present study, we evaluated the T-cells present in lymph nodes of 14 patients with MCL. We also evaluated the T-cells for co-expression of CD4/CD8 and FoxP3. Methods: Our patients consisted of 1 woman and 13 men, age range 49-78 (mean age 65), 6 previously treated and 8 de novo. Flow cytometry (FC) was run on lymph node tissue of all 14 cases to evaluate the T cells. We focused on the data from antibodies CD3, CD4, and CD8. Lymph node tissue sections from all 14 patients were selected for staining (IHC) with FoxP3 and CD4 or CD8. Results: There was heterogeneity in the CD3+ T cell populations (range 2.7-55% [mean 15.8%]). Double staining with CD4/FoxP3 (range 0.2-3.4% [mean 1.7%]) and CD8/FoxP3 (range 0-3.2% [mean 1.4%]) showed significant heterogeneity in both populations. Spearman non-Gaussian regression analysis comparing the CD4/FoxP3+ and CD8/FoxP3+ cells to total CD8 cells showed a negative correlation by both IHC and FC. Correlating CD4 Treg with CD8 by FC and IHC indicated an inverse correlation in 7 of 14 cases. Only 1 case had a positive correlation. Conclusion: These findings suggest increasing FoxP3 populations in MCL could result in a decrease in CD8 T cell immune response. Further characterization of the role of Tregs in MCL may lead to new modalities for treatment. Research supported by: Moffitt Cancer Center Department of Clinical Pathology

Abstract #: A-117 Presented by: Christopher Schrock, BS, Med II Student MS-12: A Valid Instrument for Measuring Medical Student Quality-of-Life Christopher Schrock, BS,USF, COM, Steven B. Goldin MD PhD - Dept of Surgery, Monika M. Wahi MPH - Dept of Epidemiology and Biostatistics, Michael T. Brannick PhD - Dept of Psychology, Ayesha S. Farooq,, Melissa Arce - Dept of Chemistry, Lucas R. Wiegand MD, Healther L. Carpenter BS, Heather A. Borgman AAS, Charles N. Paidas MD MBA - Dept of Surgery, Maria L. Cannarozzi MD - Dept of Internal Medicine, Lois L. Nixon PhD MLitt MPH - Dept of Internal Medicine, USF, COM, Basic Biomedical Sciences, Dept of Molecular Medicine Keywords: Quality of life; Students, Medical; Education; Surgery Objective: We developed a 14-question instrument to measure quality-of-life (QL) of medical students by selecting questions from a larger, well-validated instrument. The goal of this analysis was to examine reliability and validity of the newly-developed instrument. Methods: The QL instrument was administered to 207 third-year medical students rotating through a required 8-week junior surgical clerkship. The instrument was administered at week 1 and week 6 for each clerkship period from June 2005-September 2007. Factor analysis was computed on week 1 data to identify subscales and remove questions not loading on any subscale. Subsequently, factor analysis was computed separately using week 6 data to confirm the subscales. Chronbach’s alpha was computed to estimate subscale and total scale reliability; subscale correlations were computed. Results: Factor analysis of week 1 data identified 3 factors; 12 of the 14 questions loaded onto the 3 subscales. The subscales were designated “Fatigue” (5 questions), “Anxiety” (3 questions), and “Satisfaction” (4 questions). Analysis of the week 6 data successfully replicated the structure evident in the week 1 data. All subscales at both time periods had Chronbach alpha raw scores of >0.7. Total scale reliability was 0.83 at Week 1, and 0.85 at Week 6; correlations among the subscales ranged from 0.45 to 0.58 at Week 1, and from 0.29 to 0.50 at Week 6. Conclusion: This newly-developed 12-question instrument, the “MS-12”, demonstrates evidence of factorial validity and adequate reliability. This instrument may be useful in future studies of quality-of-life for all medical student clerkships. Research supported by: USF Department of Surgery

Abstract #: A-118 Presented by: Ashley Sterrett, MD, Resident Does Arthur teach Internal Medicine Residents Joint Pattern Recognition? Ashley Sterrett, MD, Joanne Valeriano-Marcet, MD; John Carter, MD; Frank Vasey, MD; Shanmugapriya Gnanashanmugam, MD; USF Department of Rheumatolgy. Shirrin Ahmadi, USF medical student. USF COM Keywords: Recognition of Joint Pattern Presentaions of Disease. Objective: To teach Internal Medicine Residents the pattern of joint involvement in common musculoskeletal diseases, which is integral to establishing a differential diagnoses and determining appropriate management of the patient. The more competent a physician is in these diagnostic skills, the better the care for the patient. “Arthur” is a computer-based teaching tool that teaches joint pattern recognition. This novel approach teaches residents a differential diagnosis to rheumatic conditions based on the joints involved. Methods: Internal medicine residents rotating through the Rheumatology department can voluntarily use Arthur. It is posted on Blackboard for access by the residents. Pre and Post-rotation quizzes will be given anonymously and not graded. Comparisons between the quiz results will be used to assess Arthur’s effectiveness based on score improvement and feedback. Results: Results so far have been positive both in with improvement in test scores and in the resident attitude toward the Arthur program. While very few residents have used the Arthur program, and those who did use it only used it once. The average score on the Arthur Pre-test was 52% (14.2/27) and the average score on the Arthur Post-test was 69%(18.8/27). This is a 17% improvement in scores from Pre-test to Post-test. Approximately 40% of the Internal Medicine residents reported using the Arthur program. However, this study is still early in its course. Conclusion: While initial examination of the data shows improvement in scores after exposure to the Arthur program, there is still more data to collect. Also, the use of the Arthur program has been limited, thus the improvement in the scores could also be a reflection of the exposure and teaching from the Rheumatology rotation.

Abstract #: A-119 Presented by: Donald Thometz, BA, Med III Student Is Outcome After Laparoscopic Nissen Fundoplication the Same for Patients With Disparate Indications? The Impact of Reflux vs. Dysphagia Before Fundoplication on Outcome. Donald Thometz, BA, Mark Shapiro BS, Harold Paul, Desiree Villadolid MPH, Sharona Ross MD, Donovan Tapper MD, Sarah Cowgill MD, Alexander S. Rosemurgy MD, USF, Division of General Surgery USF, COM, Department of Surgery Keywords: GERD, dysphagia, laparoscopic Nissen fundoplication Objective: This study was undertaken to compare symptomatic outcomes between patients undergoing fundoplication for the disparate indications of excessive acid reflux vs. dysphagia. Methods: Laparoscopic Nissen fundoplications were undertaken in 420 patients with predominant symptoms of excessive acid reflux and in 58 patients with predominant symptoms of dysphagia. Before and after fundoplication, patients scored the frequency and severity of heartburn, regurgitation, and dysphagia utilizing a Likert scale. Results: Before fundoplication, patients with predominant symptoms of excessive acid reflux vs. patients with predominant symptoms of dysphagia had more frequent and severe heartburn, and more frequent and severe regurgitation but less severe dysphagia (p<0.05, for all). After fundoplication, all symptoms queried improved dramatically for all patients (p<0.01). As well, after fundoplication the frequency and severity of symptoms of excessive acid reflux and dysphagia were similar between patients undergoing fundoplication for predominant symptoms of excessive acid reflux or dysphagia. Conclusion: Before fundoplication, patients with predominant symptoms of excessive acid reflux, relative to patients with dysphagia, had more frequent and severe heartburn and regurgitation and less severe dysphagia. Fundoplication dramatically improved the frequency and severity of all symptoms queried for all patients. After fundoplication the frequency and severity of all symptoms were similar between patients undergoing fundoplication regardless of predominant presenting symptoms. Disparate indications (e.g., symptoms due to excessive acid reflux or obstruction) do not impact outcome after fundoplication.

Abstract #: A-120 Presented by: Tracy Torrella, MA, Graduate Student Disparities in Utilization of Bariatric Surgery in Florida (1999-2003) Tracy Torrella,,Ali Kandil, MPH (Lake Erie College of Osteopathic Medicine-Bradenton) Jason Salemi, MPH, COPH USF, Michel Murr, MD, USF COM, Department of Surgery Keywords: Bariatric Surgery; Morbid Obesity; Utilization; Minority Health Disparities Objective: The burden of obesity is confounded in minority populations due in part to barriers in access to care. The prevalence of obesity, defined as Body Mass Index (BMI) greater than or equal to 30 kg/m2, is 28.7% in non-Hispanic Whites versus 39.9% in non-Hispanic Blacks (2000). Emerging evidence supported by the NIH indicates that operative treatment is the only effective modality that produces sustainable and durable weight loss. Therefore, we sought to assess the impact of race, age, and gender on utilization of bariatric surgery in Florida. Methods: Data on all patients undergoing bariatric surgery in Florida from 1999-2003 were abstracted from the prospectively collected and mandatory-reported statewide hospital discharge database. Data are utilization (prevalence) rates and 95% Confidence Intervals. Results: Of the 15,210 bariatric surgery patients, the majority were women (83%); 87% were White. Utilization rates (per 10,000) increased yearly for all groups from 33.1 to 149.7 (1999-2003; however, White women continued to utilize bariatric surgery at disproportionately higher rates (221.3) compared to White men (98.4), Black women (86.4), and Black men (32.4). Conclusion: Utilization of bariatric surgery in Florida increased dramatically from 1999 to 2003. Nevertheless, there is a marked racial disparity in utilization. Although the prevalence of morbid obesity is more than doubled in Blacks than Whites (9.0 vs. 4.4%), White patients utilize bariatric surgery nearly 7 times more than their Black counterparts. The public health implications are overwhelming; identifying the etiology of this disparity is a crucial step in the fight against obesity and its associated disorders. Research supported by: USF Department of Surgery

Abstract #: A-121 Presented by: Michelle Valdes, MS, Med II Student The Scope of Oral Health Practice and Needs of Child Health Care Medical Providers in Florida Michelle Valdes, Hilma Benjamin M.D., Maja Marinkovic M.D. and Rani S Gereige M.D. MPH. Department of Pediatrics, USF, COM, Department of Pediatrics Keywords: Oral health, pediatric practice, fluoride Objective: This study provides an indepth look at the pediatric oral health scope of practice of primary care providers (PCP) and residents in Florida. It also is a needs assessment for current practice, barriers, and how training issues should be addressed. Methods: A web-based survey was sent electronically to the AAP Florida Chapter listserv and resident section. The link was placed in the Florida Academy of Family Physicians electronic newsletter. Over the span of three months, four reminder e-mails we sent. The survey collected demographic data, oral health training data and practice patterns. Results: Sixty four percent of respondents were PCPs, 20% are in academic, and 47% have been in practice >15 years, and 36% were residents. Among all respondents, only 25% had an oral health curriculum in residency, 89% in the form of didactics. PCPs routinely ask about brushing habits (59%), Fluoride exposure (52%), and the availability of a dental home (77%). However, only 3-15% routinely assess mother/caregiver’s oral health in any way. A significant number reported routine dental inspection of all groups of children 0-3 as part of the PE particularly those with visible cavities but only around 7% perform Fluoride varnish application if needed. Respondents expressed interest in learning more about oral health (83%), and feel they need more training particularly in the form of hands-on workshop. Conclusion: Overall, most PCPs and residents, despite inadequate oral health training, include OHRA as part of health maintenance visits. However, the oral health scope of practice could be improved with more needed training, Fluoride varnish application, and increased awareness of the role parent/caregiver oral health plays among others. Research supported by: AAP CATCH Program.

Abstract #: A-122 Presented by: Mathew Varghese, MD, Resident Anaphylaxis After Receiving Perflutren For Exercise Contrast Stress Echocardiography Mathew Varghese,Mark C. Glaum, Richard F. Lockey USF COM, James A. Haley VA Medical Center, Tampa, FL USF COM Department of Internal Medicine Keywords: perflutren, definity, anaphylaxis, contrast, echo Objective: Perflutren, used for IV contrast enhancement during echocardiography, is available as a clear colorless, non-pyrogenic, hypertonic liquid which upon activation provides a homogeneous white suspension of lipid microspheres. Perflutren, octofluoropropane (OFP), has a biochemical structure of C3F8. Binding of OFP microspheres to protein is minimal. It becomes a gas in the circulation, is not metabolized and cannot be detected after 10 minutes in either blood or expired air. A 67-year-old male with chest pain received a contrast stress echocardiogram (CSE). Perflutren was used to better delineate the left ventricular wall and to rule out ischemia. Ten minutes after receiving perflutren, he became tachypneic, developed laborious breathing, facial flushing and erythematous macules over the trunk, arms and shoulders. Pulse-oximetry was 94%, heart rate was 130 and BP was 90 systolic. A diastolic BP could not be obtained. He received ativan 1 mg IV, diphenydramine 25 mg IV and prednisone 60 mg by mouth and began to improve within 2 minutes. CSE done 1 year prior caused no adverse reaction. Methods: A Pubmed search using the following words was completed: perflutren, Definity Highlighter ® (Bristol-Meyers, Billerica, MA), octofluoropropane, OFP. Results: One adverse reaction, hemodynamic instability, is reported in an elderly female. Conclusion: Although generally considered safe, perflutren is associated with anaphylaxis. The mechanisms for such a reaction are unknown. The patient was advised to avoid CSE with perflutren in the future. Research supported by: Department of Allergy and Immunology, USF and James Haley VAMC, Tampa FL

Abstract #: A-123 Presented by: Marie Vasher, BS, Med III Student Pityriasis Rubra Pilaris in a Mother and Son and Review of the Familial Subtype of This Disease Marie Vasher, BS, USF, COM, Erica Smithberger, M.D., Dermatology Resident, Neil Fenske, M.D., F.A.C.P., Director, Dermatology & Cutaneous Surgery, Professor of Medicine & Pathology, USF, COM, Department of Dermatology Keywords: Familial Pityriasis Rubra Pilaris Objective: We present two cases of Pityriasis rubra pilaris (PRP) arising in a mother and son and review the rare familial subtype of this disease. Methods: Four biopsy specimens from involved areas were obtained from the mother and two from the son. Two additional biopsy specimens were obtained from the son 13 months later. Each specimen was stained with hematoxylin and eosin for viewing under light microscopy. Results: All biopsy specimens revealed psoriasiform dermatitis consistent with PRP. Other findings compatible with PRP included a superficial lymphocytic perivascular inflammatory infiltrate in the dermis of the mother’s biopsy specimens, as well as in biopsies taken from the son. The son’s latter specimens demonstrated alternating orthokeratosis and parakeratosis of the stratum corneum overlying the epidermal hyperplasia, in both vertical and horizontal directions, which is very characteristic of PRP. Conclusion: The diagnosis in the two cases presented was based primarily upon the well-recognized clinical features, including follicular hyperkeratotic papules on an erythematous base in addition to red to orange-red scaly plaques with intervening “islands” of spared skin, and complemented by the histopathology. This mother and son presentation is consistent with the rare familial subtype of Pityriasis rubra pilaris, which is inherited primarily via autosomal dominance with variable expression. A mere 0 to 6.5% of those with PRP report a family history of the disease. The familial form tends to present atypically, be more gradual in onset, persist throughout life, and be one of the more treatment-resistant forms of the disease.

Abstract #: A-124 Presented by: Steve Wilson, MD, Resident Technique and Results of Percutaneous Small-Diameter Perforations for Avascular Necrosis of the Shoulder Steve Wilson, MD,German A. Marulanda, M.D., Odion Binitie, M.D., Nazeem Virani, M.D. Department of Orthopaedics and Sports Medicine, University of South Florida. Michael A. Mont, M.D, 2Center for Joint Preservation and Reconstruction, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore Keywords: Core decompression, shoulder, osteonecrosis Objective: Osteonecrosis of the humeral head occurs about 10% as often as this disorder in the hip. This study reports a new technique for treatment of shoulder osteonecrosis using multiple small percutaneous perforations. Methods: Between May, 2002, and October, 2003, 26 core decompressions of the humeral head were performed in 15 patients with early stage (pre-collapse) symptomatic osteonecrosis of the shoulder. There were 8 women and 7 men who had a mean age of 37 years (range, 15 to 50 years). Patients were followed for a mean of 32 months (range, 24 to 41 months). Clinical outcome was assessed using the UCLA shoulder score. Parameters such as age, gender, corticosteroid use, previous treatment on the same joint, and the presence of multifocal osteonecrosis(greater than 3 anatomic sites) were studied as predictors of outcome. Results: Twenty five shoulders (96%) had an excellent clinical outcome (UCLA score > 24 points) at the most recent follow-up. The mean UCLA score improved from 14 points (range, 10 to 22 points) preoperatively to 27 points (range, 14 to 30 points) at final follow-up (p0.05). Conclusion: Percutaneous core decompression is a novel low-morbidity, outpatient procedure. When performed at an early disease stage it can reliably alleviate pain, and defer the need for shoulder arthroplasty in patients with symptomatic osteonecrosis of the humeral head.

Abstract #: A-125 Presented by: Witmer, Matthew, M.D. Resident An Unusual Case of Retroperitoneal Bleeding from a Spindle Cell Sarcoma Witmer, Matthew, M.D. Khushi Dhaliwal, M.B.B.S., Candice Mateja, D.O., John Greene, M.D., Sateesh R. Prakash, M.D.USF COM and Moffitt Cancer Center Keywords: retroperitoneal, hemorrhage, spindle cell, sarcoma Objective: To report a case of retroperitoneal hemorrhage originating from a spindle cell sarcoma. To emphasize to physicians the need to rapidly identify and treat this rare, but life threatening, complication, in order to reduce mortality. Methods: We reviewed the medical records of this patient. Results: The patient is a 37 year old male, with a past medical history of a spindle cell sarcoma, presenting to Moffitt Cancer Center with profound anemia, generalized fatigue, exertional dyspnea, and left flank pain for several weeks. The patient denied any chest pain, melena, bright red blood per rectum (BRBPR), and hematemesis. The physical exam revealed mild tachycardia and generalized tenderness to abdominal palpation, but was otherwise, unremarkable. A low hemoglobin value of 6.2 g/dL was the only significant laboratory result. During the hospital course, a CT scan of the abdomen and pelvis revealed a retroperitoneal bleed originating from the spindle cell sarcoma. The patient received 4 units of packed red blood cells and interventional radiology was promptly consulted to identify and embolize the source of bleeding. Although an exact source of bleeding was not identified, the patient’s hemoglobin remained stable after embolization. Conclusion: This case highlights the importance of considering retroperitoneal bleeds in the differential diagnosis of a patient with severe anemia and no evidence of overt bleeding. Retroperitoneal bleeds, without any history of trauma or manipulation, are exceedingly rare. This potentially fatal complication of a sarcoma must be identified in order to prevent mortality.

Abstract #: A-126 Presented by: Robert Zoble, M.D., Ph.D., Faculty

Prescribing Practices of Guideline-indicated Medications in Veterans with an Acute Coronary Syndrome.Robert Zoble, M.D., Ph.D., Britta I. Neugaard, PhD, MPH – USF and Sarah Moadhadi, BS – USF. COM Department of Internal Medicine Keywords: Coronary artery disease, acute coronary syndrome, medications Objectives: The objective of this prospective study was to evaluate the quality of care delivered to veterans with an acute coronary syndrome (ACS). Methods: The sample consisted of patients admitted with an ACS to nine geographically diverse VA medical centers (five tertiary academic centers and four rural referral centers) from April 2005 to December 2006. Hospital-based chart abstractions were performed once providers confirmed the ACS diagnosis. Prescription of guideline-indicated ACS medications (aspirin, beta blockers and lipid-lowering medications) was determined and prescription of medications at admission was compared to that at discharge. Results: In the overall sample of 832 ACS patients, there was a significant increase from the active outpatient prescriptions noted at admission to prescriptions at discharge (aspirin: 61.6% to 90.4%; beta-blockers: 68.5 to 93.1%; lipid-lowering agents: 72.8% to 92.0). In the 536 patients with prior CAD based on the admission medical record, there was also significant improvement in prescriptions from admission to discharge (aspirin: 69.6% to 89.9%; beta-blockers: 83.9 to 94.2%; lipid-lowering agents 83.8% to 93.1%). Of these known CAD patients, 375 (70%) had an admission low-density lipoprotein (LDL) cholesterol at the goal of <100 mg/dL. In the 160 not at goal, prescription of lipid-lowering therapy increased from 80.5% to 91.8%. Conclusions: In those known to have CAD prior to admission, there was a high rate of prescription of beta blockers and lipid-lowering agents, and an apparent sub-optimal use of aspirin. However, this apparent low prescription rate for aspirin may have related to patients purchasing aspirin over the counter. Significant improvement in the prescription of aspirin, beta blockers and cholesterol-lowering medications occurred after an ACS admission, both for those with prior known CAD and also for those newly diagnosed. Impact Statement: Adherence to guideline-indicated medical therapy for ACS patients was very high in these VA centers and surpassed that previously reported in non-VA registries.

Session “B”

BIOMEDICAL ENGINEERING AND DRUG DISCOVERY

CANCER BIOLOGY

CARDIOVASCULAR SCIENCES

COMPUTATIONAL BIOLOGY AND TECHNOLOGY

ENVIRONMENTAL AND GLOBAL HEALTH

FAMILY AND GENERAL HEALTH

NEUROSCIENCE

OTHER CLINICAL STUDIES AND CASE REPORTS

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Abstract #: B-32 Presented by: Cindy Bucher, BS, Staff Marine Extracts with Antiviral Activity Cindy Bucher,J Alan Maschek, Department of Chemistry, University of South Florida Bill Baker, Department of Chemistry, USF CAS, Alberto van Olphen, Department of Global Health, USF COPH, Department of Global Health Keywords: Marine Extracts, Antivirals, Influenza, Screening Objective: To identify new and effective therapeutics against influenza viruses from a collection of marine natural products. With an estimated 3 to 5 million annual influenza infections resulting in as many as 500,000 deaths, there lies a critical need to identify novel antiviral drugs. Because natural products account for the origin of more than 60% of new small molecule drugs, we have initiated screening on a unique collection of more than 2,500 marine organism extracts derived from diverse habitats, including Antarctica, the Caribbean, Hawaiian, and continental US. Methods: Marine natural products were collected from multiple diverse habitats and crude extracts and fractions prepared following activity-guided fractionation. Primary Screen: Antiviral activity was determined using cell based assays consistent in light microscopy and visually scoring of cytopathic effects (CPE) and colorimetric quantification of cell viability. Secondary Screen: Dose dependant response evaluation, plaque reduction assay, one step growth progeny reduction, and cytotoxicity in mammalian cells. Results: A crude extract A-4 was identified using our primary screen a subsequent fraction confirmed to posses anti-influenza activity using the secondary screening protocol. This extract and fraction were non-toxic in vitro. The hit rate from this library is 0.7%. Conclusion: Our results support the effectiveness of our screening methods and demonstrate the value of this unique marine collection of organisms as a valuable source of biologically active compounds including antivirals.

Abstract #: B-33 Presented by: Mina Guerges, Undergraduate Electro-Gene Transfer in the Lung Mina Guerges,Megan Mabis, USF, William G. Marshall, Jr., MD, MBA, Department of Surgery, USF COM, Brian Boone, BS, USF COM, Margaret Baldwin, MS, Department of Comparative Medicine, USF Michele Danielson, BA, Department of Comparative Medicine, USF Jane Lawson, BS, Department of Comparative Medicine, USF Dennise Caretto, CVT, Department of Comparative Medicine, USF Yolmari Cruz, BS, Department of Molecular Medicine, USF COM Bernadette Ferraro, Department of Molecular Medicine, USF COM Mark Jaroszeki, PhD, Department of Electrical Engineering, USF COE Richard Heller, PhD, Department of Molecular Medicine, USF COM, Department of Surgery Keywords: Gene transfer, electroporation, lung, pulmonary Objective: This study evaluated the use of electroporation to deliver plasmids encoding genes for luciferase to the in vivo porcine lung. Methods: Six farm pigs (neutered males, weights 25 – 30 kg.) underwent medial sternotomy under general endotracheal anesthesia. Luciferase plasmid (0.2 ml) was injected in multiple sub-pleural locations, marked with titanium hemoclips, in the upper, middle, and lower lobes of the right lung. Electroporation was administered at specific delivery parameters (field strengths and pulse widths). One site of plasmid delivery without electroporation was made as control following completion of all electroporation. Animals were maintained for 48 hours and underwent injection site excision. An additional site of lung tissue, which had received neither plasmid injection nor electroporation, was excised from the lower lobe. The level of luciferase expression was quantified using commercial firefly luciferase (Sigma, St. Louis, MO). Results: Pulmonary expression was significantly increased (10X) (p<0.05 versus control) at injection sites of luciferase with electroporation at parameters of 40 V/cm field strength and 150 ms pulse width. Expression at sites with parameters of 20 V/cm and 40 V/cm and 100 ms pulse width were also markedly increased (5 – 8X vs control), but did not reach statistical significance. Conclusion: Improved gene delivery to the in vivo porcine lung has been successfully accomplished with electroporation. Further study of electroporation as a method of gene delivery to the pulmonary parenchyma is warranted. Research supported by: University of South Florida College of Medicine Feasibility Grant: Electro-Gene Transfer for Coronary Artery and Pulmonary Disease

Abstract #: B-34 Presented by: Xiangdong Hu, PhD, Postdoc Bcl-xL-Templated Assembly Of Its Own Protein-Protein Interaction Modulators Xiangdong Hu, Sameer Kulkarni(1), Lisa Malmgren(1), Jiazhi G. Sun(2), Hong-Gang Wang(2), Roman Manetsch(1)* (1) Department of Chemistry, USF CAS; (2) The H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL Keywords: Apoptosis, Bcl-xL, Protein-Protein Interactions, Lead Discovery Objective: Protein-protein interactions are central to many biological processes and represent a large and important class of potential targets for human therapeutics. Recent discovery of a variety of low-molecular-weight compounds interfering with protein-protein complexes launch and validate viable routes for a large number of new therapies. For example, several independent studies have shown that it is possible to induce apoptosis in cancer cells by designing low-molecular weight compounds binding to Bcl-xL. Herein, we report our progress towards the development of a novel lead synthesis and discovery methods that generate only active compounds. Methods: In the last decade, various fragment-based lead discovery approaches have been reported in which the biological target, a protein or a DNA fragment, is actively engaged in the assembly of its own multidentate inhibitor from a pool of smaller reactive fragments. We tested various reactions suitable for fragment-based lead discovery approaches targeting Bcl-xL. Results: In our preliminary research, both, an amidation and a triazole-forming reaction have been found to be applicable for Bcl-xL template screening. As a template, Bcl-xL demonstrated the selective formation of hit compounds from various sublibraries containing reactive building blocks. As a result, noticeable difference was observed between the incubation reactions with and without Bcl-xL. Conclusion: So far, our research has provided four good modulators for Bcl-xL from the two reactions mentioned above. Additionally, the hit compounds discovered by our novel strategy have been prepared in our laboratory and have shown convincing bioactivity against the protein Bcl-xL. Research supported by: American Cancer Society, James and Esther King Biomedical Research Program

Abstract #: B-35 Presented by: Arun Kumar, PhD, Faculty 8-Isoprostanes, As A Biomarker Detecting Oxidant Stress Arun Kumar, Yvonne Davis, Dawn Vo, Shekhar Bhansali, R.F.Lockey, Shyam S. Mohapatra USF COM, Department of Internal Medicine Keywords: ISOPROSTANES, asthma, allergy Objective: Isoprostanes, including 8IPs are a unique group of compounds for study since they are makers and mediators of oxidant stress. Presently, the biochemical and immune assays of 8-isoprostanes (8-IPs), have a number of disadvantages; they are expensive and time consuming. In addition, they require special sample preparation. In this study we used a microfluidic–nanowire approach for sensitive detection of 8-isoprostanes (8IPs), as an oxidant stress biomarker. Methods: The technology of microfabrication involved preparation of Au nanowires/working electrode, fabrication of micro-fluidic chip, and integration of Au nanowires into the micro-fluidic chip. The Au nanowire sensors were functionalizred with the antibodies to specifically capture the 8-IP from the solvent system and produce the specific electrochemical signal. Results: In the micro fluidic device, the top lid was connected with tube to introduce the sample to the gold nanowires on the chip. SEM pictures of functionalized and non-functionalized nanowires were taken. The square-wave voltametry technique was used for the estimation of 8-isoprostane. A standard curve for the estimation of 8IP was constructed. Varying concentrations of 8IP was used to determine the level of sensitivity. The results demonstrate that as low as 10pg/ml of 8IP can be detected by this method. Conclusion: Taken together these results have demonstrated that a microfluidic-nanowire device can be used to detect 8IPs sensitively as a biomarker of oxidant stress.

Abstract #: B-36 Presented by: Robin Murphy, PhD, Faculty They REALLY Are Coming For You: Robot-Assisted Point of Injury Care Robin Murphy, PhD, Dr. Jenny Burke, USF, Dr. Carolina Chang, Universidad Simón Bolívar Venezuela, Prof. Cliff Nass, Stanford, Eric Rasmussen MD MDM FACP, inSTEDD USF COE, Department of Computer Science and Engineering Keywords: point of injury care, telemedicine, robotic Objective: The objective of this Institute for Safety Security Rescue Technology thrust area is to create rescue robot systems to assist with point of injury care. Applications include soldiers trapped by snipers, civilians trapped by a disaster or a car crash. Victims of a building collapse can require 10 hours of extrication before physical contact with emergency personnel. A robot can be the only source of medical inter vention or interaction with the outside world. We are investigating diagnostic sensors, protocols, robot affect (is it comforting? play soothing music?), and human-robot interaction (mediate communication with the medical specialists and family? facilitate medical information management?). Methods: The methods used are surveys, experiments, observational studies of medical personnel using robots with simulated victims, physiological measurements, and engineering R&D. Results: The results to date are: the insertion of USF robots into five incidents, including the World Trade Center, Hurricane Katrina, and the Crandall Canyon Utah mine disaster, with the expectation of a survivor, the design and commercialization of a non-contact diagnostic sensor, a modification to the START triage protocol to accommodate the limited capabilities of rescue robots, and preliminary studies of affect in the intimate proximate zone. Conclusion: We conclude that robot-assisted point of injury care is an emerging area requiring multi-disciplinary teams consisting of experts in engineering, medical, and psychology. Research supported by: This work is supported by the National Science Foundation, the Defense Advanced Research Projects Agency, the Army Research Laboratory, and Radiance Technologies.

Abstract #: B-37 Presented by: Tina Mutka, MPH, Staff Discovery of Antimalarial Drugs from Marine Natural Products Tina Mutka,Jaime Heimbegner (b), J. Alan Maschek (b), Bill Baker (b), and Dennis E. Kyle (a); (a) Global Health Infectious Disease Research, COPH ; (b) Department of Chemistry, CAS Keywords: Malaria, drug discovery, natural products, screening Objective: The continued emergence of multidrug resistant Plasmodium falciparum underscores an urgent need for new, effective treatments. Natural products have been the source of the two most important antimalarial drugs (artemisinin and quinine), as well as for many other chemotherapeutics. Herein we report on the utility of qualitative and quantitative in vitro bioassays to guide the identification and fractionation of novel chemotypes with antimalarial activity from marine natural products. Methods: Plasmodium falciparum are grown in vitro using modified RPMI media with human plasma and erythrocytes. Extracts from a unique repository of >2500 marine microbes and invertebrates from Antartica, the Carribean, and Hawaii are screened at 100, 50 and 5 µg/mL. Parasite growth is quantified by a SYBR G fluorescence assay and data analyzed in a custom database program. Active extracts are then confirmed with quantitative concentration-response assays. Results: In the initial series of 264 extracts screened, eight extracts were determined to be Active (>50% inhibition at 5 ug/ml) and nine extracts were Partially Active. Fractionation of the Active and Partially Active extracts is underway and activity in the bioassay will guide selection of the most promising candidates for structure elucidation. Conclusion: These data demonstrate the utility of marine microbe and invertebrate sources for identification of novel natural products with potential for development as antimalarial drugs. Although <10% of the collection has been screened, the initial hit rate is 6% and activity was found in extracts from both marine microbes and invertebrates.

Research supported by: NIAID R43 AIO76051-01 and the Florida High Tech Corridor

Abstract #: B-38 Presented by: Mujiba Ansari, BS, Undergraduate The Stability of RNA in Liquid Based Cytology Samples Over A Standard Period Of Time. Mujiba Ansari, Erin Siegel PhD USF CAS, Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: RNA, Stability, Liquid based cytology Objective: The main objective of this study was to determine the stability and variance of RNA extracted from Human Cell Lines (HeLa) that are preserved in PreservCyt media, a standard preservative used for Liquid Based Cytology. Methods: Fresh cultured HeLa cells at three concentrations were preserved in PreservCyt media at room temperature (45 samples total). This study was conducted over a 5- week timed period. Every week for 5 weeks, cells at each concentration in triplicate were pelleted down and frozen at –80 C. After the completion of the 5 weeks, the frozen pellets RNA were extracted using Qiagen’s Micro Rneasy kit and quantitiated using the Nanodrop. The grouping of the extraction was conducted based on the same concentration of cells. The experiment was conducted twice.

Results: The results were conducted by using the Nugen Nanodrop, which is a full –spectrum UV/spectrophotometer used to quantify nucleic acids, proteins, fluorescent dyes and other compounds. The reading of the RNA usually stayed more consistent within the same concentration in comparison to weeks. The controls, which had a standard concentration across all concentrations, were shown to be consistent in every extraction. There were a total of 6 extractions conducted.

Conclusion: The final conclusion of this research was that the PreservCyt media did not have any effect on the degradation of the cells; rather it was the quantity of the cells that were shown to be defining factor in determining how much RNA could be extracted. Research supported by: American Cancer Society

Abstract #: B-39 Presented by: Vance Lambert, Undergraduate Localization of Anti-Cancer Activity Within the Accessory Protein Vpr of HIV Vance Lambert, Andrea N. McCray Department of Molecular Medicine USF, Karrupiah Muthamani Department of Pathology University of Pennsylvania, David B. Weiner University of Pennsylvania, J. Joseph Kim VGX Pharmaceuticals Inc. Richard Heller Department of Molecular Medicine USF, Kenneth E. Ugen Department of Molecular Medicine USF, USF COM Basic Biomedical Sciences - Molecular Medicine Keywords: Vpr Peptides Apoptosis Melanoma Objective: We, as well as others, have observed the anti-cancer activity of the HIV accessory protein Vpr (viral protein R). This activity is hypothesized to be due to its ability to initiate cell cycle arrest as well as apoptosis. The objective of the study presented here is to localize the region within this protein that mediates the anti-cancer effect , using a murine melanoma tumor model. Methods: The melanoma line B16.F10 was utilized in these studies due to its aggressive and metastatic nature and poor immunogenicity in the C57BL6 mouse model. Overlapping 15aa Vpr peptides were tested individually against B16.F10 cells in vitro in order to assess effects on cell proliferation as measured by an MTT dye assay. Results: In initial experiments, peptides spanning the carboxy region of Vpr were determined to mediate significant inhibition of B16.F10 proliferation. Three peptides spanning aa 65-83 of Vpr , i.e. labeled 17 (aa 65-79), 18 (aa 69-83), and 19 (aa 73-88) specifically inhibited cell proliferation in a dose response manner. The inhibition of B16.F10 in vitro by peptides 17,18 and 19 was 58, 56, and 35% respectively. Conclusion: These in vitro results suggest that the carboxy region of the Vpr protein can mediate significant inhibition of cell proliferation of this melanoma tumor line. These, as well as our previous results, indicate the potential clinical utility of Vpr as an anti-tumor agent. Specifically, this data suggests that smaller peptide reagents from Vpr could mediate the biological activity of the full protein. These peptide reagents, would, in principal have advantages over the use of the full 96 aa protein. In vivo studies using the Vpr protein and peptides in the B16.F10 tumor model are being pursued.

Abstract #: B-40 Presented by: Delaura Patel, Undergraduate Review of Studies Using Massage Intervention for Cancer Pain Delaura Patel,,Abinaya Chari, Jennifer Muller MPH, David Yokum BS, Cynthia Myers PhD LMT USF CAS Department of Biology - Integrative Biology Keywords: massage, cancer, pain, complementary therapy Objective: The aim of this study is to analyze and assess peer-reviewed published clinical trials examining the effect of massage on cancer-related pain. Methods: The published studies on massage as an adjunct to treating cancer pain were found by a literature search on PubMed and CINHAL. The terms massage, cancer, and pain were cross-referenced to yield 17 studies. Studies were evaluated on design, sample size, massage intervention, measurement tool for pain, time of assessment, and results. Results: Methods of massage included Swedish, Esalen, light touch, aromatherapy, and foot reflexology. Many studies used pre/post intervention assessments of pain to test the immediate effects. Six studies also included assessments of longer term effects of massage. Some studies had control groups which did not receive massage, allowing for comparison. Numerical rating scales and visual analog scales were the most commonly used methods for pain measurement. Eleven out of 17 studies reported statistically significant results (p-value ? 0.05) demonstrating a reduced pain level immediately following massage. No studies found significant results of longer term effects. Conclusion: Drawing conclusions on the effects of massage on pain is difficult due to varied methods across studies (e.g. control groups, massage style, dose) as well as the subjectivity of the pain experience. Other limitations included small sample size, statistical analysis reported, and subject attrition. These findings show a need for further study of massage for pain with larger samples, blind control and intervention groups, and qualitative investigation.

Abstract #: B-41 Presented by: Zheng Shen, PhD, Postdoc Regulation of hTERT mRNA Expression by 1,25-dihydroxyvitamin D3 through microRNA-498 In Ovarian Cancer Cells Zheng Shen,Umesh Jinwal, Santo V. Nicosia, Patricia Kruk, Xiaohong Zhang, Wenlong Bai USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: vitamin D, VDR, microRNA, telomerase, hTERT, ovarian cancer Objective: We have shown previously that vitamin D down regulates telomerase expression in ovarian cancers by decreasing mRNA stability of its catalytic subunit, hTERT. The current studies are to define the molecular mechanism underlying the down regulation. Methods: Microarray, RT-PCR, Southern blot, RNase protection, reporter assays, chromatin immunoprecipitations, EMSA, MTT assay, etc. Results: Our microarray analyses showed that 1,25-dihydroxyvitamin D3 (VD) stimulated the expression of miR-498. RT-PCR analyses confirmed the induction of miR-498 by VD. Transfection of miR-498 decreased the expression of hTERT mRNA whereas the transfection of miR-498 antisense reduced the VD-induced down hTERT regulation. Combinational reporter gene assays, chromatin immunoprecipitations, and eletrophoretic mobility shift assays identified one of two direct repeat-3 (DR3) elements in miR-498 genome as a functional vitamin D response element for miR-498 up-regulation. Sequence analyses showed that miR-498 targeted the 3' untranslated region (3`-UTR) of hTERT, leading to the down regulation of hTERT mRNA. MTT assays showed that miR-498 antisense partially relieved the growth suppression by VD in the OVCAR3 cells. Conclusion: Taken together, our data defined the molecular mechanism underlying the down regulation of hTERT by vitamin D and demonstrated the involvement of a microRNA in vitamin D action in ovarian cancer cells.

Abstract #: B-42 Presented by: Xiaoqin Wang, MD, Postdoc Targeting Natriuretic Peptide Receptor A for Treatment of Prostate Cancer Xiaoqin Wang, Xiaoyuan Kong and Subhra Mohapatra USF COM Department of Internal Medicine Keywords: Prostate, Tumor, ANP, NPRA, TRAMP Objective: Prostate cancer is the third leading cause of death among men in America. Although androgen deprivation has been found to be effective in treating androgen-dependent prostate cancer, it is ineffective in treating advanced prostate cancers, the cause of mortality. These observations emphasize the need for development of effective treatments for advanced prostate cancer. We have recently demonstrated that the receptor for atrial natriuretic peptide (ANP), NPRA, played key role in tumorigenesis of lung, skin and ovarian cancers. In this study, we present evidence that NPRA signaling is important for prostate tumor growth. Methods: The role of NPRA in modulating prostate cancer was tested using a syngenic transpalnatable tumor line derived from transgenic adenocarcinoma of mouse prostate (TRAMP) model, TRAMP-C1. For in vivo assays, both C57BL/6 (wild type) and NPRA knockout (KO) mice were injected subcutaneously with 5x106 TRAMP-C1 cells and tumor sizes were compared at the end of the experiment. The role of NPRA deficiency in survival of TRAMP-C1 cells was tested in vitro by ectopic expression of a plasmid encoding NP73-102, the NH2-terminal peptide of the ANP prohormone, which downregulates NPRA expression, or directly by small interfering RNA for NPRA (siNPRA). Apoptosis was detected by terminal transferase dUTP nick end labeling (TUNEL) assay and Western blot. Results: NPRA deficiency substantially protected mice from prostate tumor growth. Downregulation of NPRA expression by both siNRRA or NP73-102 significantly induced apoptosis in prostate cancer cells. Conclusion: The results suggest that ANP signaling via NPRA play a key role in prostate tumor engraftment and represents a new target for prostate cancer therapy. Research supported by: The Florida Department of Health

Abstract #: B-43 Presented by: Jordan Watson, BS, Undergraduate Satisfaction with Genetic Counseling among Recently Diagnosed Breast Cancer Patients Jordan Watson, Cheryl Miree(2); Jennifer Brzosowicz(3); Lindsey King(2); Gwendolyn Quinn(1,2; Susan Vadaparampil(1,2) (1)USF COM, Department of Interdisciplinary Oncology (2) H. Lee Moffitt Cancer Center and Research Institute, Health Outcomes and Behavior Program (3) Lifetime Cancer Screening Clinic and Prevention USF CAS, Department of Chemistry Keywords: BRCA1/2, Satisfaction, Genetic Counseling Objective: An estimated 5-10% of all cases of breast cancer are due to mutations in the BRCA1/2 genes, which confer a 56% to 87% lifetime risk of breast cancer. Genetic counseling (GC) and genetic testing (GT) are becoming standard-of-care options for high risk patients, such as women with a personal and/or family history suggestive of hereditary breast cancer. GC is offered to women who are newly diagnosed (pre definitive treatment), are going through treatment, or have completed treatment (post definitive treatment). For recently diagnosed breast cancer patients, results of BRCA1/2 mutation status may factor into treatment decision making. For example, women who are BRCA1/2 mutation positive may choose a bilateral prophylactic mastectomy to reduce future risk of contralateral breast cancer by 90%. The objective of this study was to determine if the level of satisfaction with GC differed between pre definitive treatment patients and post definitive treatment patients. Methods: The sample consisted of 20 female breast cancer patients who attended GC before (n=10) and after (n=10) definitive surgical treatment. All participated in a quantitative mail survey pertaining to their GC experience. Results: Results show that women who attended GC prior to definitive surgery were more satisfied with the timing of referral; however, the women who attended GC after definitive surgery were more satisfied overall with the entire experience of GC. Conclusion: Future research will use a larger sample and examine the practices of other clinics which offer GC. Research supported by: This work is currently supported by an American Cancer Society Mentored Research Scholar Grant for Dr. Susan Vadaparampil CPPB-111062.

Abstract #: B-44 Presented by: Yingtao Zhang, PhD, Postdoc Deacetylation of Cortactin by SIRT1 Promotes Ovarian and Breast Cancer Cell Migration Yingtao Zhang, Sarah Yong1, Xin Li1, Mu Zhang1, Jiandong Chen2, Patricia Kruk1, Jin Q. Cheng1,2, Wenlong Bai1,2, Santo Nicosia1 and Xiaohong Zhang1,2 * 1Department of Pathology & Cell Biology, COM USF 2Molecular Oncology Program, H. Lee Moffitt Cancer Center & Research Institute *Correspondence USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Cortactin, SIRT1, Ovarian Cancer, Breast Cancer, Cell Migration Objective: To determine a class III histone deacetylase, SIRT1 deacetylates cortactin and to examine the role of SIRT1 in tumorigenesis of ovarian and breast cancers. Methods: Immunoprecipitation (IP) and GST pull-down assay were used to study the interaction between SIRT1 and cortactin. Cell migration was determined by Transwell™ migration assays. Immunostaining was used to show the co-localization of SIRT1 and cortactin. Results: SIRT1 co-localizes, binds to and deacetylates cortactin, whereas p300 catalyzes its acetylation. SIRT1 specific inhibitor, EX-527, elevates the level of cortactin acetylation. Deletion analyses have mapped the SIRT1 binding domain to the cortactin repeat region, which is an F-actin binding domain, indicating that SIRT1 might regulate cortactin’s F-actin binding activity and cell motility. Sir2 null mouse embryonic fibroblasts (MEFs) migrate slower than that of wild type MEFs, and gain migratory ability after transfected with exogenous SIRT1, suggesting a crucial role for SIRT1 in cell migration. Also, breast and ovarian cancer cell lines expressing an acetylation mimetic mutant of cortactin are less motile whereas cells expressing the deacetylation mimetic mutant of cortactin migrate faster than that of control cells. SIRT1 is overexpressed in breast cancer cell lines and tumor tissue where SIRT1 is inversely correlated to the acetylation level of cortactin suggesting that deacetylation of cortactin is associated with high level of SIRT1 and tumorigenesis. Conclusion: In summary, our results suggest that cortactin is a novel substrate for SIRT1 and p300 and, for the first time, a possible role for SIRT1 in cell motility and tumorigenesis via deacetylation of cortactin. Research supported by: Florida Bankhead-Coley Cancer Research Program

Abstract #: B-45 Presented by: David Eve, PhD, Faculty Release of VEGF by ReNeuron Cortical Stem Cells. David Eve, James Musso, III1; Viet H. Bui1; Adam J. Smith1; Carmelinna Gemma1; Don F. Cameron1,2; Cathy Oliveira3; Kenneth Pollock3; Andrew Hope3; Marc-Olivier Baradez3; John D. Sinden3; Paul R. Sanberg1,2. 1Center of Excellence for Aging and Brain Repair, Departments of Neurosurgery, and 2Pathology and Cell Biology, USF COM ; 3ReNeuron Inc., Guildford, England. Keywords: VEGF, angiogenesis, stem cell, transplantation, stroke Objective: Previous research using the human immortalized cortical stem cell line (ReN001; ReNeuron, Inc., UK) revealed considerable benefit on long term transplantation of these cells into a stroke rat model. The mode of action may be the release of specific growth factors promoting angiogenesis and/or neurogenesis, thus stimulating the growth of cells in the infarcted area. The release of these factors was determined by protein array and ELISA analysis of the conditioned growth media of these cells. Methods: The cells were grown in their requisite media until greater than 70% confluence is reached. The media was collected, spun down to remove cell debris, rapidly frozen and stored at -80oC. The media was biotinylated and run on a RayBio® Biotin Label-based Human Antibody Array. Proteins of interest were confirmed by specific ELISAs. The angiogenic potential of the media was tested in an in vitro assay whereby the proliferation of cells from rat aorta grown in conditioned media is determined. Results: Media collected at several different passages and over 1-3 days was processed and certain proteins were consistently observed, particularly vascular endothelial growth factor (VEGF), a promoter of angiogenesis. This result was confirmed by ELISA (R & D Systems) and values ranging from 1-3 ng/ml were detected. The in vitro angiogenesis assay demonstrated extensive organized growth of capillary-like structures with conditioned media from these cells (compared with disorganized structures in unconditioned media). Conclusion: VEGF is released by these cells and their media is capable of promoting angiogenesis. We are currently investigating whether VEGF is the important factor for this process or whether other factors are involved. Research supported by: ReNeuron, Inc.

Abstract #: B-46 Presented by: Rosana Lastra Vicente, Staff Title: Folate Rescues Inhibitory Effects of Wnt/β-Catenin Signaling During Heart Development Rosana Lastra Vicente, M. Han, M. Serrano, J.C. Huhta, K.K. Linask Dept. Pediatrics, USF/ACH Children’s Research Institute, St. Petersburg, FL Keywords: Wnt/β-catenin signaling, homocysteine, folic Acid, heart defects Objective: The canonical Wnt/β-catenin signaling pathway is critical in early embryonic development. Lithium (Li) has been shown to mimic Wnt/β-catenin signaling by inactivating glycogen synthase kinase-3β (GSK-3). Chick and mouse embryos exposed to Li during gastrulation develop severe heart, including valve, defects. Folate (FA) deficiency leads to elevated homocysteine (HCys) levels, which in turn lead to comparable heart and valve defects as Li exposed embryos. This suggests an intersection between HCys/FA metabolism and Wnt/β-catenin signaling. To further analyze this possible intersection between Wnt and Hcy/ FA metabolism, FA was tested for protective effects during cardiogenesis, similar to its protection against neural tube defects. Methods: MF20 immunostaining for sarcromeric myosin heavy chain and in situ hybridization for early cardiac gene expression were used to evaluate FA effects on cardiac cell differentiation. Avian embryos were treated with Wnt3A at 2 and 10 ng/mL and with FA at 2 and 10 μg/mL. Results: Results indicate partial FA rescue of cardiogenesis at concentrations of 2μg/mL of FA, and total rescue with 10μg/mL. To initiate inducers of cardiogenesis, Wnt antagonists act on the mesendoderm to upregulate the genes Hex/Islet. Exposure of avian embryos to Li/HCys inhibited Hex/Islet expression in the cardiogenic crescent, thus augmenting inhibitory Wnt signaling. With FA supplementation, the cardiac inducers Hex/Islet are reexpressed in the heart fields, and in the mouse normal heart development was observed in HCys exposed embryos.

Conclusion: In conclusion, FA supplementation was shown to override Wnt3A/Li/HCys inhibition of heart development and gene expression, potentiating the repression of canonical Wnt/β-catenin signaling.

Abstract #: B-47 Presented by: Maria Serrano, MD, Faculty One-time Homocysteine Exposure in Early Gestation Causes Intrauterine Demise and Cardiac Valve Dysfunction Maria Serrano, MD,James C Huhta MD, Mingda Han MD, Kersti K Linask PhD USF/ACH Children's Research Institute USF COM Department of Pediatrics Keywords: Cardiac function Homocysteine Echocardiography Valve regurgitation Objective: Cardiac abnormalities are associated with elevated homocysteine (Hcy) concentrations. Our aim was to evaluate the effect of one-time Hcy exposure in early pregnancy on mouse embryonic cardiovascular findings. Methods: Compare eight pregnant mice (C57BL/6) receiving an intraperitoneal injection of 125 µL of 75 µmol Hcy and seven controls (0.9% NaCl) (C) on E6.75. On E15.5 the mice were anesthetized with isoflurane and embryonic color directed pulsed wave Doppler (echo) was performed. Maternal heart rate (HR) and uterine (UtA) pulsatility index (PI) were calculated plus PI of descending aorta, umbilical, and ductus venosus. From the inflow-outflow velocity waveforms the outflow velocity (OFV) and the cardiac cycle time intervals including isovolemic contraction and relaxation times were measured; myocardial performance index was calculated. Semilunar (SL) or atrioventricular (AV) valve regurgitation (VR) was recorded. CRL, body and placental weight were measured. Results: Forty Hcy and 36 C embryos were studied. Maternal HR (455 vs 431 beats/min; p=0.01) and the UtA PI (2.95 vs 2.67; p=0.02) were higher in the Hcy vs C group. Embryonic OFV (30-84, mean 42.9 vs 22-46, mean 36.6 cm/s; p=0.003) was higher in Hcy vs C. Seventeen (42.5%) of the Hcy group had SL VR and 6 (15%) had AV VR, versus none in C. There were 32.5% (13) embryonic resorptions in the Hcy group and none in C. All Hcy embryos had lower placenta/body weight ratio (0.30 vs 0.34; p=0.001) and shorter CRL (15 vs 18 mm; p<0.0001). Conclusion: Pulsed Hcy exposure in early embryonic life increased the risk of intrauterine demise and was associated with placental growth failure and cardiac VR and outflow obstruction. Research supported by: Supported by AHA0555321B to KKL and PCRC51-6302 to JCH

Abstract #: B-48 Presented by: Aysha Ahmed, Undergraduate Impact of Technology on Medical Education: a 15-year review Aysha Ahmed, Aliyah Baluch MD, Sateesh Prakash MD USF CAS, Department of Chemistry Keywords: technology in medical education Objective: In 15 years technology has been an important component of medical education. Advances in technology include, but are not limited to, computers, the Internet, and the World Wide Web. The objective of this study is to determine how technology has impacted medical education from 1992 to 2007. Methods: Extensive literary searches were done to evaluate the impact of technology on medical education from 1992 to 2007. Results: In the early 90’s the articles focused on the introduction of computers and the Internet into medical schools. In 1992 Randall and Burden stated that medical education required direct communication between students and instructors. However, by 1998 opinions shifted when Masys’ study proved that although the student-teacher interaction is very important, the integration of technology could be an asset rather than a liability to medical education. In 1999 Devitt took it further to prove that students learn better with computer-aided lectures vs. traditional methods. In 2004 technology went beyond computerized lectures and into real time videos and educational networks such as Blackboard, and Oasis as they exist in USF. In 2007 Duggan’s study showed that electronic voting systems could be used in classrooms to improve student learning by encouraging them to attend lectures. So in 15 years we went from the introduction of technology into medical curriculum to having technology become an integral part of medical education. Conclusion: In 15 years technology was incorporated into the medical curriculum slowly and now is integral to the learning process. From this search of advances of technology in medical education we encourage medical schools to continue to use these resources to the advantage of their students.

Abstract #: B-49 Presented by: Saqib Ahmed, Undergraduate Discovering the Perfect Medical Record: A Comparison of Two Medical Records Saqib Ahmed, Aliyah Baluch, M.D. Sateesh R. Prakash, M.D. USF CAS, Department of Chemistry Keywords: Record keeping, paper medical charts, electronic medical charts, advantage, disadvantage Objective: Paper medical charts (PMCs) have been indispensable in providing vital information for patient care. However, in recent years a new form of record keeping has emerged, i.e. electronic medical records (EMRs). Our study compares both systems. Methods: An extensive search to review medical information discussing record keeping systems that covered articles from 1997 to 2007. We evaluated PMCs and EMRs in order to see the advantages and disadvantages of both systems. Results: Currently PMCs are used by most practices. The benefits include minimal electronic dependency, and low cost. Disadvantages include variations in penmanship and the inaccessibility of records to those outside of the office thus making PMCs time intensive. EMRs are an emerging technology, currently used by approximately 20% of practices. Though the costs are significantly higher than PMCs, EMRs offers instant access to information without issues with legibility. In a study, U.S. hospitals that switched to EMRs saw a 66% drop in prescription errors. It has been shown that EMRs tend to be 40% more complete and retrieved up to 20% faster than PMCs. Conclusion: Although both systems offer advantages and disadvantages, overall EMR keeping is the superior technology. Despite the elevated initial costs, long term use shows a significant savings, in which a five year model showed a savings of $86,000 per provider.

Abstract #: B-50 Presented by: Jaclyn Bostick, Undergraduate Are USF Medical Students Selecting the Most Satisfying Medical Specialties? Jaclyn Bostick, Aliyah Baluch, MD Ren Chen, MD, MPH Sateesh R. Prakash, MD USF CAS Keywords: physician satisfaction, medical specialities, USF Objective: In the US, thousands of 4th year medical students are challenged with decision of selecting a medical specialty. Research has revealed a significant difference in satisfaction among the various medical disciplines. Our study evaluated the relationship between national specialty satisfaction and selection choice among graduating USF medical students. Methods: We reviewed a national physician survey published in 2002 to identify satisfaction among various medical disciplines. We then obtained the 2001-2003 match list for graduating seniors at USF and made a comparison. A statistical analysis was performed to evaluate a correlation. Results: There was no significant correlation between national medical specialty satisfaction and USF medical student’s career choice (p=0.2914, Pearson correlation=0.43). There was also no statistical correlation between medical specialty selection and distribution in the nation compared to USF (p=0.7197, Pearson correlation is 0.1519). Conclusion: Our study illustrates that graduating USF medical students are not selecting the most satisfying medical specialties. Our data also shows that USF graduating seniors reflect a difference in specialty distribution in comparison to national physician distribution. Diversity at USF may account for this variation.

Abstract #: B-51 Presented by: Saya Karim, Undergraduate Bluetooth: An Emerging Technology in Medicine Saya Karim,Aliyah Baluch, M.D. Sateesh R.Prakash, M.D. USF CAS, Department of Biology - Integrative Biology Keywords: technology, bluetooth, medicine Objective: Bluetooth is a novel form of short range wireless communication. Initially introduced in the late 1990’s, Bluetooth has evolved to become the fastest growing technology in the world. Research regarding its utility in the medical field is sparse. Our study evaluated the comprehensive uses of Bluetooth in medicine. Methods: An extensive search was performed to identify the various and growing roles of Bluetooth in the medical field. A search from 1998-2007 was conducted across all disciplines of medicine to produce a detailed list of its uses. Results: Our study revealed that Bluetooth is utilized in several medical fields ranging from orthopedics to cardiology. In addition, all health care providers including physicians, nurses, paramedics, and health educators have greatly benefited from Bluetooth. It improves efficiency by eliminating the need for cables. It also provides mobile medical data acquisition through direct access into electronic medical records and decreases medical errors. Examples of its uses include: bluetooth enabled databases, pulse oximeters, wireless EKG monitors, defibrillators, heart montitors, scales, and spirometers. The following devices have been recently introduced: Avant 4000 wireless pulse reader, Lifesync wireless electrocardiogram, MedApps D-PAL, Audiovox6700, and Medtronic’s Lifepak 12. Bluetooth has advanced all devices by improving speed, communication, and efficiency. Conclusion: Technological advances are equally as important as biological sciences in the ongoing progress for improved patient care. Every minute saved using technology like Bluetooth potentially saves another life.

Abstract #: B-52 Presented by: Opal Raj, BS, Undergraduate Knowledge, Attitudes, and Concerns of Premedical Students to PGD Opal Raj,Cheryl Miree, M.S. (1), Lindsey King, M.P.H. (1), Dr. Susan Vadaparampil, Ph.D.(1,2), Dr. Gwendolyn Quinn, Ph.D.(1,2) (1)-H.Lee Moffitt Cancer Research Institute Health Outcomes & Behavior (2)-USF COM, USF CAS, Department of Biology - Cell Biology, Microbiology and Molecular Biology Keywords: Preimplantation Genetic Diagnosis, Students Objective: Preimplantation genetic diagnosis (PGD) is a test used to identify a variety of genetic disorders in embryos developed or created through in vitro fertilization (IVF). Results are used to inform prospective parents about the genetic status of an embryo before implantation into the uterus; however, the procedure has raised numerous concerns. This study aims to examine the knowledge, attitudes, and concerns of premedical students towards PGD. Methods: A scan-format 26 question survey was distributed to approximately 270 students in 2 classes at the University of South Florida. Student participation in this 10 minute survey was completely voluntary and anonymous. Results: The survey was administered in the summer of 2007 and had an overall response rate of 88.5% or 239/270. Of these 239 students, 224 were interested in pursuing a career in a health-related field. Of the 224 students, 74.1% had never heard of PGD. Given a broad definition of PGD, most believed PGD was an acceptable option for families with a genetic history and would recommend it to a patient. The largest concern students had was that PGD could be easily used for the wrong purposes. The majority believed that its greatest benefit is for parents to improve the chances that their child will be free of genetic mutations. Conclusion: Premedical students are limited in knowledge concerning this technology, but this did not affect their recognition of the technology’s value or their decision to recommend it. Healthcare leaders must absolutely be informed of current technologies and treatment options to better enhance the practice of medicine in the U.S. today. Research supported by: This work is supported in part by the American Cancer Society Institutional Research Grant # 93-032-10.

Abstract #: B-53 Presented by: Sanju Wadhwa, Undergraduate Defibrillator Use in Europe and U.S.A. Sanju Wadhwa, Sateesh R. Prakash M.D., Aliyah Baluch M.D. USF Keywords: Defibrillator Death Rates Effective Objective: Our aim here is to compare the implantation information available for Europe and USA since we have somewhat similar health related expenditures. Methods: The information/data was collected from the case-control studies, published articles, and observational data using various key words on search engines such as google.com, pubmed.com and medscape.com. Results: Defibrillators studied in the European Society of Cardiology and the European Resuscitation Council in their studies (6) together suggested that a tiered response system increases survival rates. Their study showed an overall survival rates of between 55% survival. Among subjects in whom VF was the initial recorded rhythm, the overall survival to hospital discharge rate was 53%, but survival was 74% for those patients who had the first shock 3 minutes from collapse. Conclusion: Whereas a few clinical studies have been specifically designed to address the economics issue at the present time, there are only rough estimates of the cost involved (9). In the OPALS study, a cost of 46,900 US$ per life saved was calculated for establishing the early defibrillator program and 2400 US$ per life saved annually for maintaining the program. They concluded that the cost-effectiveness of placing AEDs on commercial aircraft compares favorably with the cost-effectiveness of widely accepted medical interventions.

Abstract #: B-54 Presented by: Monica Gray, MS, Graduate Student Sustainable Control of Ascaris lumbricoides (worms) in a Rural, Endemic, and Developing Community – A Systems Approach Monica Gray, MS,,Noreen Poor, Ph.D USF College of Engineering Civil and Environmental Engineering Keywords: Ascaris lumbricoides, Developing Community, Systems Approach, Sustainability, Solar Latrine Objective: 1. Development of a STELLA® model that includes the human-parasite relationship, nutritional status assuming that a substantial portion of the diet comes from soybean, and management of human excreta; 2. Design of a high-rate solar latrine to determine the extent to which pathogens can be predictably de-activated in human excreta. This project combines environmental engineering, public health, and education. Methods: This research proposes a systems approach as the more sustainable solution to reducing parasitic infections. The systems thinking, dynamical software, STELLA® is used to simulate time-dependent changes in a village population in response to sanitation interventions such as use of latrines, including pit and solar latrines, and recycling human wastes to soybean crops. Results: 1. In endemic communities, the parasite regulate the human population. 2. A latrine intervention can destablize the human-parasite relationship. 3. The incidence of parasitic infection can increase after a latrine intervention. 4. Properly designed solar latrines can be used to reduce worm burden in endemic communities. Conclusion: 1. Host population will grow exponentially until limited by resources or disease. 2. The parasites may regulate or destabilize the host population depending on the distribution of pathogen among host members. 3. Sanitation interventions should be carried out simultaneously with hygiene education and improvement in nutrition to be sustainable. 4. Human waste recycling may be sustainable if the waste stream is sufficiency free of pathogens.

Abstract #: B-55 Presented by: Giffe Johnson, MPH, Graduate Student Characterization of Cancer Risk Associated with Measured Airborne Benzene Levels in the State of Florida G. Johnson, S.C. Harbison, J. McCluskey, and R. Harbison Center for Environmental/Occupational Risk Analysis and Management, COPH USF, Department of Environmental & Occupational Health Keywords: benzene, risk assessment, leukemia, air pollution Objective: To evaluate the ambient airborne benzene exposures present in the State of Florida and to characterize the risk of developing leukemic cancers as a result of these exposures. Methods: This investigation uses historic air monitoring data from six counties in the State of Florida to characterize the cancer risk from airborne benzene concentrations utilizing current Federal and State regulatory risk characterization methodologies, and a comparative risk analysis based on occupational epidemiologic evidence. Airborne benzene samples were collected from 24 air toxics monitoring sites in Broward, Duval, Orange, Miami-Dade, Hillsborough, and Pinellas counties. From 2003 to 2006, air samples (n = 3,794) were collected using 8, 12, and 24 hr samples with sub-ambient pressure canister collectors consistent with EPA benzene sampling methodology. Results: The mean benzene concentrations, by site, ranged from 0.18 - 3.58 ppb. The resulting cancer risk estimates ranged from 4.37 x 10-6 to 8.56 x 10-5, which exceed the FLDEP’s acceptable cancer risk level of 1 x 10-6 for all monitoring sites. The cumulative lifetime exposures were calculated in ppm-years, by site, and ranged from 0.04 - 0.70 ppm-years. A comparative analysis with available epidemiological literature revealed that the association between benzene exposure and cancer risk is related to cumulative exposures in excess of 1 ppm-years. Conclusion: The results of this investigation indicate that it is unreasonable to expect additional cancer cases in Florida residents due to measured ambient airborne benzene levels, despite the fact that all regulatory risk calculations exceed acceptable cancer risk levels in the State of Florida.

Abstract #: B-56 Presented by: Tunisia Riley, MA, Graduate Student HIV/AIDS, Drugs and Mental Health in the Black Community: Links, Myths, Realities and Disparities Tunisia Riley, MA, USF, College of Arts and Sciences Women's Studies Keywords: HIV/AIDS, Drugs, Mental Health, Blacks Objective: This paper will address the links between HIV/AIDS, drugs and the mental health of the black community through social science, a feminist standpoint, and scientific data from the 1990s through 2001. This paper will also address the disparities of gender within the black community as it relates to HIV/AIDS and drug use and how the medical community, both mental and physical health professionals have directly and indirectly contributed to the rising numbers by their inability to address the issues unique to a diverse black community dying from an HIV/AIDS epidemic of vast proportions. Methods: This paper is a literature review which uses social science, a feminist standpoint, and scientific data from the 1990s through 2001. Results: To “blame the victims” of the HIV/AIDS crisis is to ignore the complexity of its nature which involves a multilateral approach to a solution. Conclusion: Community extends beyond the actual black community suffering from the epidemic to black professionals of diverse fields to work together towards a solution. Ultimately, individuals will have to make a choice but what resources and facts are provided to them before making that choice? Are they aware of the consequences of their actions and were their actions the result of mental illness, drug use coercion, fear or perceived survival? While Collins may point to the black community for its vital role in finding solutions to quelling the epidemic, evidence points to this and the involvement of all in order to fortify that community. Only a united effort both internally and externally that recognizes the complexity of those infected or affected by it can truly address and assist in stabilizing the HIV/AIDS epidemic in the African American community.

Abstract #: B-57 Presented by: Ryan Michael, BS, Graduate Student Modelling Of The Environmental, Economic And Socio-Cultural Dynamics Of Mercury Ryan Michael,, Amy L. Stuart, Ph.D., Environmental and Occupational Health, Joniqua Howard and Maya Trotz, PhD, Civil and Environmental Engineering, Trina Halfhide and Fenda Akiwumi, Ph.D., Geography USF College of Public Health, Environmental & Occupational Health Keywords: Mercury cycling, Methylmercury, industrial ecology, systems modeling, sustainability Objective: Due to its high mobility, mercury is a global pollutant whose effects may be far removed from the source of emissions. Deleterious human health effects include kidney damages, impaired mental function, and neurological disorders, particularly to the fetus. The objective of this study is to develop integrative understanding of the social, cultural, economic, and environmental factors affecting community sustainability related to mercury use, contamination, and human exposures. Methods: The methods used in this study include systems modeling, field measurements, and populations surveys. First, we are developing and applying systems models, using STELLA software, for mercury cycling in Tampa Bay and Guyana. The models will include environmental, economic, and socio-cultural subsystems. We are also measuring mercury concentration in environmental media and conducting population surveys in collaboration with other researchers. Results: To date we have made reconnaissance trips to Guyana and in the Tampa Bay area in order to gather data for modeling studies, to form collaborations, and to select population survey sites. We have developed a preliminary STELLA model for mercury in the environment and economy of Guyana. We have also identified appropriate analytical methods for determination of mercury in samples of environmental media. Conclusion: Preliminary investigations reveal that, whereas industrial emissions may have large impacts in some locations, others are affected by small-scale economic socio-cultural and religious activities. A sustainable solution to mercury pollution likely requires a balance between economic, cultural and social, and environmental needs. Research supported by: University of South Florida, Sustainable Healthy Communities grant.

Abstract #: B-58 Presented by: Euna August, MPH, Graduate Student An Exploratory Study of Role Strain and Social Support Among Graduate Health Students with Children Euna August, MPH, Cheryl Vamos, MPH (1), Cara de la Cruz, MPH (1), Teri Malo, MPH (1), Rasheeta Chandler, MSN (2), Heather Clayton, MPH (1) (1) USF COPH Department of Community & Family Health 2) USF CON Keywords: graduate students, parents, role strain, social support Objective: Universities have experienced an increase in non-traditional students, who tend to be older, have children, and be married/divorced. Students who have multiple roles also experience multiple demands that may affect their wellness and academic achievement. Therefore, understanding this population is important for recruitment and retention. However, minimal research has been conducted. The purpose of this study is to understand the experiences of graduate health student parents in balancing their multiple roles. Methods: This study was based on two theoretical frameworks – symbolic interactionism and social support. Twelve semi-structured interviews were conducted with graduate health students who are parents of at least one child seven years old or younger. Results: Participants identified multiple roles (e.g. parent, spouse, student, and role model) and prioritized the parent role. Lack of time, finances, childcare, and school support increased role strain, whereas social support from partner/spouse, family, and friends were essential in coping with responsibilities. Conclusion: Universities should implement strategies to decrease role strain and facilitate support for these non-traditional students. The availability of low cost, high quality daycare services and family health insurance is critical in supporting graduate student parents in the achievement of their parenting responsibilities along with their academic requirements.

Abstract #: B-59 Presented by: Heather Blunt, MPH, Graduate Student Assessing the Mental Health Status of Uninsured Women Attending Public Sector Clinics Heather Blunt, MPH, Daley, E., PhD, MPH, Woodard, L., MD, Edmonds, A., MS, ARNP, Fanning, J., PhD, ARNP, Dyer, K., BA USF COPH, Department of Community & Family Health Keywords: Mental Health, Insurance, Prescription Medication, Health Status Objective: Uninsured patients frequently rely on primary care as their only means of healthcare, including mental health care. Research has shown that people living below the poverty level are unduly affected by mental health issues and stress. Issues with mental health and stress can also affect other health areas. Methods: A total of 435 women completed surveys based on Healthy People 2010 guidelines for the prevention of chronic diseases, including the mental health issues. The surveys were read to patients attending clinics, and items related to mental health (2 items), exercise (3), self-reported general health status (1) and prescription drug use (3) were analyzed. Results: Approximately 45% of women (n=196) reported being depressed in the past year and more than one quarter (n=109) reported depression in the past month. Women who experience depression in the past month and/or the past year have lower self-ratings of general health, as well as lower reported exercise. In addition, women who reported experiencing depression in the past year also reported higher levels of using prescription drugs in a way that was not prescribed, and taking some else’s medication. These women also reported taking less medicine than was prescribed. Conclusion: These results have important implications for the health and safety of uninsured women reporting depression; strategies for interventions are needed. Research supported by: AHEC Women's Health Collaborative

Abstract #: B-60 Presented by: Karen Dyer, BA, Graduate Student Ethnic Disparities in Chronic Disease in a County-Level Women’s Health Needs Assessment Karen Dyer, Coreil, J., PhD; Vamos, C., MPH, Department of Community and Family Health, COPH USF Keywords: women's health needs assessment chronic disease health disparities Hillsborough County Objective: Researchers at USF College of Public Health were appointed by the Hillsborough County (FL) Commission on the Status of Women to prepare a report reviewing issues, trends and needs within the county and to make recommendations for improving women’s health. This poster highlights the ethnic disparities evident in chronic disease indicators in the county. Methods: Data were collected through 20 semi-structured interviews with local experts, and through published reports consisting of national, state and county data. Results: Various key issues in women’s health were identified, including the increased need for services due to population growth; unequal distribution and access to services and transportation; and underutilization of the noteworthy low-income county health plan. A major finding is that ethnic disparities cross-cut many health issues in the county, especially in the realm of chronic disease. For example, African American women were found to have more than twice the rate of cardiovascular disease, diabetes and obesity and nearly three times the rate of diabetes-related death compared to Caucasian women. Other chronic disease disparities highlighted include higher rates of general cancer mortality, cervical cancer incidence, and mortality from stroke. Conclusion: A needs assessment of this nature is essential in providing baseline health data for the county and will allow policymakers to address disparities at a local level. As ethnic disparities in chronic disease incidence and management occur within socio-cultural, economic and political contexts, recommendations to address risk factors were made in conjunction with those designed to expand access, alleviate public transportation barriers, and increase health plan enrollment.

Abstract #: B-61 Presented by: Roxana Eftekhari, COPH Grad Student Improving Healthcare for Refugee Children in the Tampa Bay Area: American Academy of Pediatrics CATCH (Community Access to Child Health) grant Eftekhari, Roxana , Tiffany Crawford, USF COPH Department of Global Health. Florida Center for Survivors of Torture (a program of the Gulf Coast Jewish Family Services), Haitian American Alliance Keywords: refugee healthcare access, pediatric advocacy Objective: Refugees are legal immigrants from around the world forced to flee their homelands because of threats to their lives or actions of torture against them. 80% of refugees are women and children, and Florida has more refugees than any other state. They face multiple barriers to healthcare and the goals of this project include developing and administering a survey to the local refugee population to assess the sufficiency of healthcare among their children. Ultimately, we hope to determine the more influential barriers these children are facing, then focus our efforts on improving their access to healthcare. Methods: Initially, we developed affiliations with local organizations familiar with the refugee population and who were interested in working with USF pediatric residents. With these community partners, we were able to access a large number of refugees and also use their skilled translators to administer surveys. We questioned the frequency of healthcare visits of children in the family, their medical needs, their financial coverage, the comfort of the parents in bringing their children to see a physician, and reasons why they do or do not use our healthcare system. The surveys were translated in 5 languages and the translators are calling a list of about 200 refugees. Results: Currently, the surveys are still in process. Once complete, they will be analyzed to determine the barriers that most commonly prevent these children from receiving the care they deserve. Conclusion: We plan to use the results to educate healthcare workers and to design projects within the residency program to address those barriers preventing refugee children from receiving appropriate medical and dental care.

Abstract #: B-62 Presented by: Lianne Estefan, MPH, Graduate Student The Need For Community Responses For Long-Term Survivors Of Domestic Violence Lianne Estefan, MPH, Martha Coulter, Dr.P.H., Department of Community and Family Health & Director, Harrell Center for the Study of Family Violence; Carla VandeWeerd, Ph.D., Department of Community & Family Health and Associate Director, Harrell Center for the Study of Family Violence USF COPH Keywords: domestic violence; community response; social determinants Objective: This study has three objectives; to: 1) identify community-level issues that impact the overall health and life stability of domestic violence survivors in the long term; 2) understand how interventions focused on individual issues do not adequately address these concerns; and 3) develop policy recommendations that will focus on prevention through practical supports to survivors of domestic violence. Methods: This longitudinal, descriptive study examined a community-based collaborative program that provides concurrent legal and case management services to victims of domestic violence and their children. Bivariate analyses were performed in the areas of health care, social support, finances, and transportation. Based on these results, logistic regression was performed on four outcomes, representing social determinants of health: earning enough to support the family; difficulty accessing health care; difficulty finding suitable housing; and homelessness due to domestic violence. Results: Logistic regression revealed that factors related to employment were predictive of several outcomes, including homelessness. Difficulty with transportation was a strong predictor of the ability to access health care. Having health insurance was significantly predictive of both earning enough to support the family and ease of accessing health care. Conclusion: Results indicate that while individual-focused intervention is effective in helping survivors attain positive outcomes in some areas, the most salient issues cannot be prevented or resolved through individual supports. Rather, legislative reforms are needed, including the areas of financial assistance and housing, to provide necessary practical supports for survivors who are trying to rebuild their lives.

Abstract #: B-63 Presented by: Hollie Fuhrmann, MA, Graduate Student Searching for Emergency Contraception Information on the Internet: An Observational Study of College Students’ Experiences Hollie Fuhrmann, MA, Sarah A. Smith, BA, MPH/MA , CFH & Anthropology; Eric R. Buhi, PhD, Assistant Professor, CFH; Ellen M. Daley, PhD, Assistant Professor, CFH USF COPH Department of Community & Family Health Keywords: Emergency Contraception; Health Information; Internet Search Behavior; Sexual Health Information Objective: Young people are using the Internet as their primary source for sexual health information, especially for sensitive topics like emergency contraception (EC). Yet, no research has assessed how young people go about finding such information or the quality of information retrieved. This study seeks to answer these concerns with regards to EC, and situate the results within the larger context of EC access, knowledge, and behavior. Methods: Thirty-four college students were purposively recruited from Introduction to Anthropology to reflect the USF's student body composition. Participants completed a questionnaire and used the Internet to answer an EC sexual health scenario crafted by a panel of experts. Camtasia was used to examine their online search strategies, whether they found an accurate answer, and the websites on which answers were found. Descriptive statistics were generated using SPSS 15.0. Results: 52.9% of respondents reported that they have never searched for EC information, and 32.4% reported seeking EC information online, more than any other source. All 34 participants found the correct EC information online in an average of 1 minute, 40 seconds and 4.07 clicks. The most common answer websites were www.go2planb.com and www.ec.princeton.edu. Some of the websites visited provided the correct answer, but also misinformation. Conclusion: As demonstrated in the EC literature, college students lack EC knowledge. Based on our findings, young people in need of EC information are going online. Researchers should critically examine the nexus between uncomfortable sexual health topics, like EC, and the Internet. Research supported by: This work was supported, in part, by the University of South Florida Internal Awards Program under Grant No. 18300-640500-R060576 (Buhi).

Abstract #: B-64 Presented by: Hannah Helmy, BA, Graduate Student Identifying Barriers to Cervical Cancer Prevention: A Qualitative Study with Key Stakeholders Hannah Helmy, Dyer, Karen (BA), Chandler, Rasheeta (MS,FNP-BC), Anstey, Erica (MA), Daley, Ellen (PhD, MPH), Buhi, Eric (PhD, MPH), Baldwin, Julie (PhD) USF COPH Department of Community & Family Health Keywords: cervical cancer, prevention, women's health Objective: The state of Florida has unique barriers to the prevention of cervical cancer. The purpose of this qualitative study is to identify challenges facing key informants who work within the realm of cervical cancer prevention, the results of which will inform statewide interviews that will ultimately be used to generate recommendations that will help guide activities designed to reduce the burden of cervical cancer in Florida. Methods: Data related to cervical cancer incidence and mortality were obtained from a previous phase of the study and used to establish criteria to identify high-risk counties (n=22), two-thirds of which were designated as rural by the state. Key informants from the fields of medicine and public health were identified through public health care settings and subsequently interviewed. Results: Preliminary findings from interviews with key informants indicate that barriers to cervical cancer prevention in the state of Florida are extensive, and include: poverty, lack of health insurance, lack of transportation, inconvenient clinic hours, racial and ethnic discrimination, and fear of deportation among undocumented populations. Conclusion: Key informant interviews have revealed important insights into the barriers impacting the prevention of cervical cancer in Florida. In particular, the unique circumstances affecting rural and urban communities and their differences and similarities bear important consequences on cervical cancer prevention efforts. The next phase of the study, conducting phone interviews with public health care providers statewide, will be informed by these insights and results will be presented at the annual Governor’s Conference on Women’s Health. Research supported by: Merck Pharmaceuticals

Abstract #: B-65 Presented by: Stephanie Kolar, MS, Graduate Student Dietary Supplement Use Among Low-income, Uninsured Patients Attending Public Sector Medicine Clinics Stephanie Kolar, MS Daley, E., PhD, MPH, USF Health Woodard, L., MD, Fanning, J. PhD, ARNP, Edmonds, E., MS, ARNP, Ward, J., MPH, USF Health, USF COPH, Department of Epidemiology & Biostatistics Keywords: uninsured, low-income, dietary, supplement, vitamin Objective: In the 1999-2000 National Health and Nutrition Survey 52% of adults reported taking a dietary supplement in the past month. This analysis examined dietary supplement usage among 246 patients attending Public Sector Medicine Clinics from 2005-2007. This population was uninsured and low-income; 75% were aged >40. Methods: Frequencies of dietary supplement usage as well as type of supplements used were tabulated. Chi-squared analysis was used to determine if there were significant differences between supplement usage by demographic and lifestyle variables. Supplement usage was also correlated with reported fruit, vegetable, and calcium containing foods intake. Results: Forty one percent of participants reported that they used supplements. Multivitamins were the most frequently used supplement (54% of those who used supplements). Calcium was the next frequently reported supplement used (15%). Supplement use varied significantly by (1) age group, (2) if they reported doing physical activity such as walking, jogging, or swimming in the past week, and (3) in women, whether they had received a mammogram in the past year. Whether they saw a health care provider regularly and their perceived general health were nearly significant (0.07 and 0.08). Supplement use was slightly, positively correlated with fruit, vegetable, and calcium intake (Cronbach’s alpha= 0.30). Conclusion: Supplement usage in this population was lower than national usage and varied by demographic and lifestyle factors. In this population, which has no health insurance, increasing supplement use may be a means of preventing illness. Research supported by: Area Health Education Center

Abstract #: B-66 Presented by: Abraham Salinas, MD, MPH, Graduate Student Adaptation of a Parent Training Program for Hispanic families: The HOT DOCS (c) Case Abraham Salinas, MD, MPH, Heather Curtiss, PhD, Daniela Chiriboga, MD, Jillian Williams, MA/CAS, NCSP USF COPH< Department of Community & Family Health Keywords: Program adaptation, parent training, positive behavioral support, Hispanic caregivers, children behavior problems, program evaluation Objective: This study examines how a parent training program using positive behavioral intervention support was adapted for Hispanic parents of young children with challenging behavior living in Hillsborough County, Florida. Methods: Seven steps were followed: (1) incorporation of bilingual and bicultural staff; (2) training in the curriculum; (3) translation of the curriculum; (4) Preliminary adaptation; (5) Implementation of HOT DOCS © Español; (6) Revision and refinement; and (7) Program Evaluation. Results: Cultural elements were incorporated to the curriculum and tailored to the population served. Both process and outcome evaluation results suggest that HOT DOCS is a culturally accepted intervention for this population. Fidelity and fit were balanced to create a totally new program for Hispanics while incorporating all the essential elements of an evidence-based approach in children’s mental health services that is both family centered and culturally competent. Conclusion: This study provide an example of issues to be considered in the adaptation of programs targeting Hispanic families as well as issues to be considered in the daily practice of health professionals. Research supported by: HOT DOCS (c) Grant from the Children's Board of Hillsborough County

Abstract #: B-67 Presented by: Emily Shaffer, MA, Graduate Student How Adolescents’ Mental Health Predicts their Physical Health: Unique Contributions of Subjective Well-Being and Psychopathology Emily Shaffer, MA,Shannon Suldo, Ph.D., Kathy L. Bradley-Klug, Ph.D. USF College of Education, Psychological and Social Foundations Keywords: Physical Health Subjective Well-Being Psychopathology Adolescents Objective: What is the magnitude of the associations between mental health (subjective well-being and pyschopathology) and physical health among a general sample of youth? Is physical health more strongly associated with positive (SWB) or negative (psychopathology) indicators of mental health? Which indicator(s) of mental health is driving the effect? Methods: Participants: Self-report measures collected from 401 early adolescents. Measures: Students’ Life Satisfaction Scale (SLSS: Huebner, 1991), Positive and Negative Affect Scale for Children (PANAS-C: Laurent et al., 1999), Youth Self-Report Form of the Child Behavior Checklist (YSR: Achenbach & Rescorla, 2001), Child Health Questionnaire (CFQ-CF87: Landgraf, Abetz, & Ware, 1999). Results: Results of Pearson Product moment correlation coefficients demonstrated that in a general sample of early adolescents, physical health status is associated with every indicator of mental health. Results of simultaneous regression analyses demonstrated that subjective well-being accounts for 29% of the variance in physical health; knowledge of psychopathology adds an additional 1%. Indicators of mental health that have unique associations with physical health are positive affect, followed by global life satisfaction and internalizing psychopathology. Conclusion: The current study provides the first examination of both positive and negative indicators of mental health in relation to physical health among youth. Underscores the particular salience of emotional symptoms and affect to health. Implications for promoting wellness among youth will be shared. Research supported by: Carol Lilly, M.D., USF Pediatrics Division of Child Development, Kathleen Armstrong, Ph.D., USF Pediatrics, Division of Child Development

Abstract #: B-68 Presented by: Sarah Smith, BA, Graduate Student Sources of Health and Sexual Health Information: Where Do Young People Go When They Have Questions? Sarah Smith, Hollie J. Fuhrmann, M.A., MPH/PhD Student, CFH-MCH/Applied Anthropology; Eric Buhi, Ph.D., Assistant Professor, CFH; Ellen M. Daley, Ph.D., Assistant Professor, USF COPH, Department of Community & Family Health Keywords: Sexual Health Information-Seeking Online Objective: Online health information sources have grown exponentially, as well as young people’s use of such sources for learning about embarrassing topics. Our research objective was to understand college students’ past online health and sexual health information seeking behaviors, and to learn what sexual health topics are sought online most frequently. Methods: As part of a broader observational research study, we administered a confidential self-reported survey to 34 undergraduate students, aged 18-19, enrolled in Introduction to Anthropology courses. Of the students who were interested, we purposively selected those who made our sample representative of the USF student body. The paper-and-pencil survey, self-administered in an office setting, asked respondents about their Internet experience, past online health information searching behavior, and demographic characteristics. We calculated statistics using SPSS 15.0. Results: 82.2% of respondents reported using the Internet several times per day. Of 17 health information topics listed on the survey, information about sexual health was the most frequently reported health topic sought online (76.5%). Popular sexual health topics sought online included STIs, preventing pregnancy, contraception, genitalia, and normal sexual behavior, presented in order of their popularity. Conclusion: Based on our findings, college students are using the Internet as a primary source of information for sexual health topics, and most other health topics. More research regarding their search behaviors, Internet and health literacy, as well as the quality of information they retrieve is needed. Research supported by: This work was supported, in part, by the University of South Florida Internal Awards Program under Grant No. 18300-640500-R060576 (Buhi).

Abstract #: B-69 Presented by: Kamilah Thomas, MPH, Graduate Student Assessing The Role Of Family In African American Men's Preventive Health Behaviors: A qualitative pilot Kamilah Thomas, MPH, Katrina J. Debnam, MPH, CHES -Johns Hopkins University Clement K. Gwede, PhD, MPH, RN- H.Lee Moffitt Cancer Center and Research Institute, COPH USF, Department of Community & Family Health Keywords: Social Influence; Family; African American men; Health Seeking; Health Disparities Objective: The objectives of this research is to explore the role that significant family members can play in the preventive health behaviors of African American men in the United States. Methods: Study investigators identified a convenience sample of men and women from three African American churches in Maryland, Pennsylvania, and Florida (Men n=15,Women n=17). Women were asked about their perceptions of men's health seeking and men were asked about their perceptions of their own health seeking and that of other men. Data analysis was completed using the transcribed audio recordings of the focus groups and the hand-written notes. Results: In addition to barriers,three primary themes emerged including church and social networks serve as family, major life events serve as a catalyst for health seeking, and family values and customs influence men's health seeking. Conclusion: This study provides evidence for the importance of family influence on African American men's health seeking behaviors.

Abstract #: B-70 Presented by: Cheryl Vamos, MPH, Graduate Student A Historical Review of a Florida Coalition: All Women's Health: A Florida Partnership for Change Cheryl Vamos, MPH,, August, E., MPH, Dyer, K., BA, Fuhrmann, H., MA, Richman, A., MPH, Wathington, D., MD, MPH, FAAFP, Jeffers, D., MPH, RN, Daley, E., PhD, MPH, Mahan, C., MD, Berry, E., MA, Tackett, H., MPH USF COPH, Department of Community & Family Health Keywords: women's health, coalition, advocacy Objective: A unique coalition has been recently formed to address the special policy, service delivery and advocacy efforts required for improving women’s health in Florida. The purpose of this study is to historically depict the development of All Women’s Health: A Florida Partnership for Change (AWH) and to describe the coalition’s activities related to policy and advocacy. Methods: Coalition documents including the mission, vision, meeting minutes and other operational reports will be reviewed to illustrate various initiatives undertaken by AWH in the form of timelines and tables. Results: AWH originated from the Florida Statewide Meeting on Women’s Health in 2005, which brought together statewide service providers, academicians and other diverse women’s health advocates to bridge expertise and experiences and to foster future collaborative initiatives. Since the initial meeting, organizational and leadership efforts of the coalition have performed the following activities: agreed upon an organizational name; identified mission and guiding principles; formed a Board of Directors; secured incorporated, non-profit status; launched an official website; submitted an organizational grant proposal; and successfully convened three general coalition meetings, one corporation unveiling breakfast, and one focused advocacy meeting which achieved consensus to work on two future projects. Conclusion: Even though the AWH coalition is still in its infancy, various visionary and concrete short-term and long-term goals have been achieved. AWH serves as a collective voice for both health providers and consumers and strives to improve women’s health in Florida through leadership, advocacy, education and policy activities.

Abstract #: B-71 Presented by: Jillian Williams, MA/CAS, Graduate Student Caregivers’ Perceptions of the Effectiveness of the HOT DOCS© Parent Training Program: A Pilot Study Jillian Williams, MA,Kathleen Armstrong, PhD, NCSP Department of Pediatrics Child Development USF College of Education, Psychological and Social Foundations Keywords: parent training, problem-solving, PBS, outcomes Objective: To evaluate participants’ perceptions of the effectiveness of the HOT DOCS parent training program. Methods: The design was a 1-group, pre/post design of 146 caregivers (children ages 1-10 yrs) who participated in HOT DOCS between 8/06-4/07. Measures included a knowledge test, behavior rating scales, weekly progress monitoring forms for caregiver behavior at home, and a program evaluation survey. Results: Dependent means t-test showed significant increases in participant knowledge. Chi-square analyses showed that participants' perceptions of the severity of children's problem behaviors was significantly higher than expected and their perceptions of the deficits in children's adaptive behavior was significantly greater than expected before beginning HOT DOCS. 2-factor repeated ANOVA's showed a significant main effect for time indicating significant decreases in participant perceptions of the severity of children's problem behaviors following participation in HOT DOCS. Caregivers reported skills as easy to use on the first two days of the week, followed by a midweek peak in difficulty, and a reduction in difficulty the final two days of the week. The majority of participants reported high levels of satisfaction with various aspects of HOT DOCS. Conclusion: HOT DOCS© appears to be a promising early intervention program that could be delivered in group format. Future directions for research include modifications that could be made to the program to improve participant outcomes and increase the validity and reliability of program evaluations, including changes to measurement instruments and data collection procedures. Research supported by: Dr. Kathi Armstrong, PhD (HOT DOCS author), Department of Pediatrics Dr. Carol Lilly, MD, Department of Pediatrics

Abstract #: B-72 Presented by: Jose Abisambra, PhD, Graduate Student A Low-Density Lipoprotein Receptor Isoform Lacking Exon 4 Is Present In Low-Density Lipoprotein Receptor Knock-Out Mouse Brain Jose Abisambra, Jaya Padmanabhan (Byrd Alzheimer's Institute and USF), Inge Wefes (USF), Peter Neame (Byrd Alzheimer's Institute and USF), and Huntington Potter (Byrd Alzheimer's Institute and USF) USF COM Keywords: Low-density lipoprotein receptor, apolipoprotein E, cholesterol, exon splicing, Alzheimer's disease Objective: A recent study analyzing alternatively spliced isoforms of low-density lipoprotein receptor (LDLR) reported that human cerebral tissues express mRNA encoding LDLR without exon 4 (?E4). The most commonly used LDLR-/- mouse was created by interruption of exon 4. Therefore, it is paramount for our LDLR-related Alzheimer's studies to determine if this LDLR-/- mouse produces a functional ?E4 receptor. Methods: We reverse-transcribed mRNA extracted from brain and liver of LDLR-/- and control mice. The resulting cDNA was amplified using primers that reflect the size difference of full-length and ?E4 LDLR. In addition, Western blot and immunohistochemical (IHC) assays with different anti-LDLR antibodies were performed to determine LDLR expression levels in the brains of these mice. ?E4 affinity for apolipoprotein E (ApoE) was determined by binding assays using DiI-conjugated, glial-derived ApoE. Results: We report that LDLR-/- mice express ?E4 mRNA in brain. Western blot analysis identified a cerebral LDLR species that is expressed in LDLR-/- mice at a comparable level to that found in controls. However, the amount of hepatic LDLR is significantly lower in LDLR-/- mice. IHC staining with anti-LDLR antibody revealed a similar expression pattern in both the LDLR-/- and control brains. Finally, we found that LDLR-/- brain was able to bind ApoE in functional assays. Conclusion: 1) LDLR-/- mice express a LDLR isoform that lacks exon 4. 2) The protein levels of ?E4 in LDLR-/- mice brains are similar to the amount of LDLR in brains of controls. 3) The pattern of LDLR staining in the brains of LDLR-/- and control mice are similar. 4) The ?E4 isoform in LDLR-/- brain is able to bind glial-derived ApoE. Research supported by: Johnnie B. Byrd, Sr. Alzheimer's Center and Research Institute

Abstract #: B-73 Presented by: Chuanhai Cao, BS, Faculty Title: Caffeine Reverses Cognitive Impairment And Decreases Brain Aβ Levels In Aged Appsw Mice: Implications For Alzheimer’s Treatment. Chuanhai Cao, G. W. Arendash1, T. Mori2, M. Mamcarz1, A. Dickson1, M. Runfeldt1, K. Rezai-Zadeh3, J. Tan3, C. Cao1, H. Potter1 1The Byrd Alzheimer's Inst., Tampa; 2Inst. of Med. Science, Saitama Med. Ctr., Saitama, Japan; 3Dept. of Psychiatry & Behav. Med., USF

Keywords: Alzheimer’s disease, Amyloid beta, caffeine, transgenic mice, behavior

Objectives: We have recently shown that APPsw mice given a moderate level of caffeine intake (the human equivalent of 5 cups of coffee per day) are protected from development of otherwise certain cognitive impairment and have decreased hippocampal β-amyloid (Aβ) levels due to suppression of both β-secretase (BACE) and PS1 expression (Neuroscience 142:941, 2006). Thus, we concluded that long-term caffeine intake may delay or reduce the risk of Alzheimer’s Disease (AD).

Methods: To determine if caffeine intake can have beneficial behavioral and neurohistologic effects in “aged” APPsw mice already demonstrating confirmed cognitive impairment, we administered caffeine in the drinking water (0.3 mg/ml) of 18-19 month old APPsw mice that had been pre-tested and shown to be impaired in the radial arm water maze (RAWM) task of working memory. At 4-5 weeks into caffeine treatment, animals were re-tested in the RAWM and euthanatized immediately following behavioral testing at 20-21 months of age. Results: The working memory impairment exhibited by control transgenic (Tg) mice given normal drinking water was reversed by caffeine treatment (Tg+Caff) to the extent that performance of aged Tg+Caff mice was essentially identical to that of non-transgenic controls. In addition, Tg+Caff mice had substantially reduced hippocampal (-40%) and entorhinal cortex (-46%) Aβ deposition. However, long-term caffeine treatment did not affect synaptophysin immunoreactivity in hippocampal CA1 and CA3 regions of aged APPsw mice. Other neurochemical and blood measures from these same animals will also be presented. In a related study that involved caffeine administration to normal, non-transgenic mice from young adulthood through old age, cognition function tested in old age was not improved by life-long caffeine intake. Thus, the cognitive reversal provided by long-term caffeine intake to aged APPsw mice is less likely to involve generalized mechanisms (e.g., increased glucose utilization) and more likely to involve our previously-established effects directly on Aβ production. Conclusions: Even with pre-existing and substantial Aβ burdens, aged APPsw mice exhibited working memory restoration, suggesting a treatment potential of caffeine in cases of established AD. In concert with our prior work indicating a strong protective effect of caffeine intake on cognition in APPsw mice, the robust treatment effect of caffeine demonstrated in the present study has laid the foundation for our recently-initiated clinical trials with caffeine in aged individuals.

Abstract #: B-74 Presented by: Niki Carty, MS, Graduate Student Increased Gene Expression of Neprilysin using AAV and Convection Enhanced Delivery Decreases -Amyloid Load in APP+PS1 Mice Niki Carty, Mercer M. 1, Meyers C2. , Nash K.2, Muzyckza N2, Gordon M.N.1, Morgan D1. 1Alzheimer’s Research Laboratory, USF COM Department of Pharmacology and Molecular Therapeutics, 2 Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville FL, USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Amyloid, Gene Therapy, Viral Vector Objective: Gene therapy using adeno-associated viral (AAV) vectors are an effective means of delivering transgene encoding for proteases capable of degrading Abeta (A?) which include neprilysin, ECE, and IDE to regions in the brain affected by AD pathology. Methods: In this study the convection enhanced delivery (CED) method using the step-design cannula was used to deliver adeno-associated viral vectors expressing either a native form of human neprilysin gene (NEP-n) or an engineered secreted form of neprilysin gene (NEP-s) into the right hippocampus and right cortical regions of the mouse brain. The control group was treated with a rAAV vector expression a mutant neprilysin gene (NEP-m) product with a point mutation in the active site rendering it inactive. Histology was performed to measure NEP gene expression and distribution. Results: Histochemistry for NEP revealed strong expression throughout the hippocampus in animals treated with the NEP-n and NEP-m vectors. Animals treated with the NEP-s showed similar results but expression was seen in a smaller area within the hippocampus compared to the NEP-n treated group. Histochemistry for total Abeta was significantly decreased in animals receiving the NEP-n and NEP-s viral vectors when compared to control animals in both the hippocampus and cortex. Congo red staining followed a similar trend revealing significant decreases in the hippocampus and the cortex for NEP-n and NEP-s treatment groups compared to control. Conclusion: This data suggest that increasing the expression of certain endogenous A degrading enzymes through gene therapy using the CED cannula provides and efficient means of increasing transgene distribution and may provide is a promising therapeutic avenue through which to treat AD.

Abstract #: B-75 Presented by: Rafael Eduardo Chaparro, MD, Graduate Student Caspase 3 Inhibitor Improves Neuroprotection up To 14 Days after Ischemic Event in a Rat Model of MCAO under Isoflurane or Propofol anesthesia. Rafael Eduardo Chaparro, MD, Carolina Quiroga MD, USF - MPP Diana Erasso BS, USF - Neurosciences Erik Benton, MD. USF -Anesthesiology Dave Morgan PhD, USF - Neurosciences Devanand Mangar, MD. USF -Anesthesiology Enrico Camporesi MD. USF –Anesthesiology, USF COM Keywords: Neuroprotection Caspase 3 inhibitors Propofol Isoflurane Hypoxic damage Objective: The neuroprotective effect of the anesthetics last for short periods of time. The use of caspase inhibitors has shown to be an effective method to decrease the apoptotic reaction and as a result reduce the hypoxic damage and infarct size Methods: Male Sprague Dawley rats were subjected to permanent focal cerebral ischemia. The control group received no treatment following the surgery. Group 2 received 90 minutes of isoflurane distributed by an induction chamber. Group 3 received Propofol for 90 minutes. Group 4 received the isoflurane treatment for 90 minutes followed by 3 systemic injections of a caspase 3 inhibitor which were given immediately after isoflurane treatment, 24 hours after isoflurane treatment, and one week after isoflurane treatment. Group 5 received Propofol for 90 minutes followed by the same caspase 3 treatment regimen that group 4 received. On day 14 the animals were euthanized, perfused, and the brains were harvested for analysis of infarct size using H&E histochemistry. To assess possible treatment-related differences in pathology, the infarct volumes for each treatment group were determined by the Cavalieri method. Results: The control group displayed an average infarct size of 34 mm3, while the groups treated with anesthetics alone showed no significant differences in infarct size compared to the control group. However, the addition of caspase 3 inhibitors to the anesthetics, isoflurane and Propofol resulted in up to 50% reductions in infarct size 14 days after permanent focal cerebral ischemia. Conclusion: The combination of propofol or isoflurane plus a caspase 3 inhibitor has a neuroprotective effect in Sprague Dawley rats two weeks after permanent focal cerebral ischemia.

Abstract #: B-76 Presented by: Diana Erasso, BS, Graduate Student Effect of Isoflurane or Propofol Anesthesia on Adult neurogenesis in Young and Old Rats Diana Erasso, Adam Bachestetter Department of Pharmacology and Physiology USF , Rafael Chaparro Department of Pharmacology and Physiology , Carolina Quiroga Department of Pharmacology and Physiology, Enrico Camporesi, Department of Anesthesiology USF, Samuel Saporta, Department of Pathology and Cell Biology USF University of South Florida College of Medicine Basic Biomedical Sciences - Pathology and Cell Biology Keywords: neurogenesis, anesthesia, memory, aging, hippocampus Objective: Clinical studies have shown postoperative cognitive impairment in elderly persons following anesthesia. We tested the hypothesis that general anesthesia, induced with isoflurane or propofol, contributes to this cognitive impairment by altering neurogenesis in the aged hippocampus. Methods: 4 month and 21 month male Fisher 344 rats were anesthetized for 3 hours with either isoflurane (1.5%) or propofol (35mg/kg/hr). Additional young and old rats exposed to room air or propofol vehicle served as controls. Approximately 30 minutes after anesthesia all rats were injected with Bromodeoxyuridine (BrdU; 50mg/kg) as a marker of neurogenesis. A novel appetitive olfactory learning test was used to assess learning and memory. After 7 days, rats were perfused with 4% paraformaldehyde, the brain removed, cryopreserved in 20 % sucrose and serial frozen sections (30µm) collected. BrdU immunopositive cells were counted using unbiased stereology and correlated to the performance of rats. Results: 13 rats received isoflurane anesthesia (6 young and 7 old), 6 received propofol (3 old and 3 young), 10 rats served as isoflurane control (6 young and 4 old), and 6 rats received 10% intralipid as propofol controls (3 young and 3 old). There was a significant difference between learning and retention in young and old rats. However, a significant difference in retention was not seen between rats that did or did not receive anesthesia within each age category. Conclusion: Old rats were significantly learning-impaired after isoflurane anesthesia. Research supported by: Department of Anesthesiology, University of South Florida

Abstract #: B-77 Presented by: LaTia Etheredge, BS, Graduate Student Growth Factor Dependent Changes In The Secretome Of Keratocytes LaTia Etheredge,,Bradley Kane 1, and John Hassell 1. USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: Extracellular Matrix (ECM) Objective: Fibrillar collagens and keratan sulfate proteoglycans are the major components of the cornea stroma and must be replaced by keratocytes during wound healing to ensure corneal strength and transparency. We evaluated the effect of IGF-I, TGF-ß, FGF-2, and PDGF rate of proliferation and ECM synthesis by primary bovine keratocytes. Methods: Keratocytes were collagenase-isolated from bovine corneas and cultured with either DMEM/F12, or DMEM/F12 supplemented with either 10ng IGF-I, 2ng TGF-ß, 10ng FGF-2, or 10ng PDGF/ml for 10 days, all with ascorbate. The growth of the cells was determined by cyquant assay. The level of ECM components synthesized by cells and secreted into the media was determined by 3H glycine incorporation into papain and collagenase sensitive protein and western blots with protein-specific antibodies to procollagen I and III, ?SMA, fibronectin, and keratocan. Results: αSMA, fibronectin, and keratocan. Compared to control cultures, IGF-I stimulated cell number by 0.7 fold, fibrillar collagen by 10.4 fold, and keratocan by 16.6 fold; TGF-β stimulated cell number by 1.8 fold and fibrillar collagen by 4.3 fold, but inhibited keratocan by 80%; FGF-2 stimulated cell number by 3.5 fold, but inhibited fibrillar collagen and keratocan by 60%; PDGF stimulated cell number by 2.4 fold, fibrillar collagen by 5.2 fold, and keratocan by 3.7 fold. Conclusion: IGF-I and PDGF increased levels of keratocan while TGF-? induced the highest levels of fibronectin. FGF-2 inhibited synthesis of collagen and keratocan. This suggests that the levels of synthesis of ECM components are growth factor dependent and that IGF-I and PDGF may be useful in restoring normal ECM synthesis by keratocytes during corneal wound healing. Research supported by: NIH Grant EY08104

Abstract #: B-78 Presented by: Jennifer Jernberg, BS, Graduate Student Spirulina Prevents the LPS Induced Decrease in Hippocampal Cell Proliferation Jennifer Jernberg, A. D. Bachstetter 1,3 , A. Schlunk 2, J. L. Vila 1, C. Hudson 4, M. J. Cole 1, C. Gemma 1,4, P. C. Bickford 1,4 1 Center of Excellence for Aging and Brain Repair, Dept. of Neurosurgery, USF, 2 Honors College USF, 3 Molecular Pharmacology and Physiology, USF, 4 James A. Haley VA Hospital, Tampa, FL, USF COM Department of Neurosurgery Keywords: Neurogenesis Neuroinflammation astrocyte spirulina Objective: Neuroinflammation has been shown to be a potent negative regulator of stem cell and progenitor cell proliferation in the neurogenic regions of the brain. During aging there is an increase in neuroinflammation which appears to negatively impact neurogenesis, as well as, learning and memory. Recently we demonstrated that decreasing a key neuroinflammatory cytokine IL-1? in the hippocampus of aged rats reversed the age related cognitive decline and decrease in neurogenesis. The objectives of this study were to determine if an acute systemic inflammatory insult of lipopolysaccharide (LPS) could affect neural stem/progenitor cell function and if the effect may be related to astrocyte activation. In addition could the effects of LPS be prevented by a diet supplemented with 0.1% spirulina. Spirulina is a blue-green algae which has antioxidant and anti-inflammatory properties. Methods: Before being injected intraperitoneally with LPS young rats were fed for 30 day a control diet or a spirulina diet. The day after the LPS insult the rats were injected with BrdU (50mg/kg b.i.d. i.p.). Quantification of the BrdU positive cells in the subgranular zone of the dentate gyrus demonstrated a decrease proliferation of the stem/progenitor cells in the hippocampus as a result of the LPS insult. Results: The results demonstrated that a diet supplemented with spirulina was able to negate the LPS induced decrease in stem/progenitor cell proliferation. Conclusion: These results support the hypothesis that a diet which is enriched with spirulina may help protect the stem cell populations of our body from the negative effects of inflammation. Research supported by: This work was supported by the National Institutes of Health (AG024165A, AG04418) and the VA Medical Research Service.

Abstract #: B-79 Presented by: Lori Lebson, MS, Graduate Student Monocyte Gene Therapy for the Treatment of Alzheimer’s Disease APP+PS1 Transgenic Mice Lori A. Lebson1, Siddharth G. Kamath3, Kevin Nash2, Nick Muzyczka2, Dave Morgan1, Marcia N. Gordon1 1 Alzheimer’s Research Laboratory, Department of Molecular Pharmacology and Physiology, USF COM 2 Department of Molecular Genetics and Microbiology, UF, COM, Gainesville, Florida 3 Moffitt Cancer Center & Research Institute, USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Alzheimer's disease Gene Therapy Monocytes APP + PS1 Objective: Recent in vitro and in vivo studies demonstrate that the up-regulation of A?-degrading enzymes can significantly reduce the accumulation of the A? peptide. Identification of a method to up-regulate brain neprilysin activity may provide a new therapeutic potential. Methods: For the study, we utilized bone marrow harvested aseptically from 8-10 month old C57BL/6-Tg mice that express GFP. Nine month old APP+PS1 transgenic mice received 5 x 106 GFP+ CD11b+ monocytic cells injected into a microvascular port once a week for two months and the mice were sacrificed one day after the last injection. Group 1 received monocytes that were transfected with a neprilysin construct in which the membrane binding domain was replaced with a signal peptide triggering secretion sequence, appended with HA tag (NEP-S-HA). This construct generates a secreted form of neprilysin in transfected cells. Group 2 served as the untreated control group and provided a baseline for immunohistochemical measurements. Results: Fluorescent immunostaining for GFP and HA qualitatively demonstrated that the monocytes had been transfected with neprilysin and had migrated to the brain in the vicinity of the plaques. Significant decreases in A? staining were measured in the frontal cortex and hippocampus between the treated mice and the control group by computer-assisted image analysis. There were also decreases in Congo red staining between the treated and control group. Conclusion: Therefore, manipulating monocytes to express an amyloid-degrading enzyme such as neprilysin offers a powerful novel therapeutic tool for the treatment of Alzheimer’s disease. Research supported by: Technical support provided by Margi Baldwin. Supported by AG 15490.

Abstract #: B-80 Presented by: Mackenzie Ott, MS, Graduate Student Functional Interactions And Responses Of Retrotrapezoid Nucleus And Ventral Respiratory Column Neurons To Central Chemoreceptor And Baroreceptor Stimulation Mackenzie Ott, MS,,Sarah C. Nuding, Ph.D. - USF, Kendall F. Morris, Ph.D. - USF, and Bruce G. Lindsey, Ph.D. - USF Prof and Chair Dept. Mol. Pharm. & Phys. USF COM Basic Biomedical Sciences - Molecular Pharmacology and Physiology Keywords: Respiratory, Central Chemoreceptor, Baroreceptor, Retrotrapezoid Objective: The retrotrapezoid nucleus (RTN) is a proposed site of central chemoreception, but its interactions with the ventral respiratory column (VRC) remain poorly understood. We tested the hypothesis that RTN neurons responsive to central chemoreceptor and/or baroreceptor stimulation have functional connections with neurons of the VRC. Methods: Multi-electrode arrays were used to record extracellular potentials from neurons in the regions of the RTN (n = 70) and VRC (n = 71) of decerebrate, vagotomized, neuromuscularly blocked, and artificially ventilated cats. Spike trains were evaluated for respiratory related discharge patterns and changes in firing rates during stimulation of central chemoreceptors by injection of CO2-saturated saline solution into the vertebral artery and during arterial baroreceptor stimulation by inflation of an embolectomy catheter in the descending aorta. Results: Respiratory modulated neurons were found in both regions: RTN: 58, VRC: 49. The majority of RTN neurons responded to chemoreceptor (59/70 = 84%) or baroreceptor (42/70 = 60%) stimulation. Crosscorrelograms from 413 of 4,920 neuron pairs evaluated had significant primary offset features indicative of functional paucisynaptic interactions within and between both regions (RTN to RTN: 35 peaks, 21 troughs; RTN to VRC: 41 peaks, 18 troughs). Conclusion: Interpretations of the data suggest both excitatory and inhibitory actions of RTN neurons upon the VRC appropriate for modulation of the respiratory pattern. Results also support the hypothesis that interactions within the RTN integrate and transform central chemoreceptor and baroreceptor inputs. Research supported by: NS46062 NSF-NIH Collaborative Research in Computational Neuroscience Program and grant NS19814.

Abstract #: B-81 Presented by: Paul Shapshak, PhD, Faculty The Use Of siRNA Nanotechnology for NeuroAIDS: HIV-1 and Host Gene Expression. Paul Shapshak, PhD, USF COM Dept of Psychiatry; Wilson Xu, PhD, Shyam Mohapatra, PhD, John Sinnott, MD; USF COM, Frank Fernandez, MD; , Int. Med, Psychiatry & Beh Med; Robert Duncan, PhD, E. Margarita Duran, MS U of Miami Miller Med. School, Epidemiology, Psychiatry & Beh Sci,;, Elyse Singer, MD, Andrew Levine, PhD UCLA Med. School, Neurology; Karl Goodkin, MD, PhD UCLA Med. School & Cedar Sinai Med Center Psychiatry,; Deborah Commins, MD USC Med. School, Neuropathology,; Fabiana Ziegler, MD.Louisiana State University Med. School, Int Med/Peds,

Keywords: NeuroAIDS, Gene expression, siRNA, Nanotechnology Objective: 1.Identify genes with significantly changed gene expression in neurons isolated from basal ganglia (BG) by laser capture microdissection (LCM) comparing HIV Associated Dementia (HAD) and encephalitis vs. HIV-1+ individuals. 2.Produce siRNA against HIV and host gene expression use nanotechnology delivery. Overall goal is to develop treatment alternatives to vaccines for HIV-1 Associated Neurocognitive Impairment & Disorder. Methods: 1. We analyzed gene expression in BG neurons from 19 AIDS brain + and - HIV associated dementia (HAD) and + and - HIV encephalitis (HIVE) (four groups). We produced the neurons using LCM. Gene expression in pooled LCM'd neurons from each case was analyzed on Microarrays. 2. In separate cell culture experiments, siRNAs were designed specifically for the HIV-1B LTR, tat, rev, and gag mRNAs. Results: 1. ANOVA showed 197 statistically significant genes among four groups (p ? 0.002). The genes represented included transcription and stress-related pathways (e.g. MAPK11, IL1Receptor-like ligand, and beta amyloid precursor binding protein). A non-stochastic number of these genes associated with Chromosome 22 (p ? 0.0001). 2. siRNAs against HIV LTR, tat, rev, and gag knocked down HIV-1B replication in cell cultures. Conclusion: Bioinformatics identified pathways involved in HAD. miRNA gene controls may exist and chromatin may be damaged on chromosome 22 in HAD and HIVE. These processes may exacerbate the effects of disease during transcription as well as due to interaction with other gene expression pathways. This sets the stage for the application of siRNA nanotechnology to knock down HIV gene expression as well as specific key host genes. Research supported by: DA04787, DA07909, DA12580, DA14533, AG19952, GM056529.

Abstract #: B-82 Presented by: John Arledge, MD, Resident Clinical Spectrum of Variant Achalasia: Diagnostic & Therapeutic Challenges John Arledge, MD, H. Worth Boyce M.D. USF medical staff, David Estores M.D. USF USF COM, Department of Internal Medicine Keywords: Achalasia esophageal motility esophageal spasm Objective: Achalasia is a disease best defined by esophageal manometric criteria. Classic features of achalasia include esophageal body aperistalsis & failure of lower esophageal sphincter (LES) to relax during deglutition. These can result in failure of esophageal function and progression to a life-threatening condition. It is important to clearly define manometric variants of achalasia to prevent the misdiagnosis of nonspecific esophageal dysmotility an untreatable but benign condition. Manometric variants of achalasia include high amplitude simultaneous esophageal contraction, complete but ineffective LES relaxation, & short segment esophageal aperistalsis. Our definition of the variant (VA): 1. Presence of short segment peristalsis in the distal esophagus. 2. Aperistalsis in the remainder of the esophagus. 3. Other achalasia variations can be present. Methods: Retrospective chart review of all motility studies at the USF Center for Esophageal & Swallowing disorders with the diagnostic code “achalasia variant”. Exclusion criteria include medical or surgical conditions supported by scientific literature as altering motility. Charts with motility reports mentioning short segment peristalsis were de-identified & reviewed by an esophagologist. Results: Review of 900 patients diagnosed with achalasia yielded 16 possible candidates with VA. Blinded review revealed correct identification of the diagnosis in 12 out of 16 cases, yielding a sensitivity & specificity of 75% & 86% respectively. Conclusion: This study identifies & defines VA which has not been adequately described in medical literature. Esophageal manometry which reveals findings of VA with retained short segments of peristalsis should be acknowledged as a variant of achalasia.

Abstract #: B-83 Presented by: Melanie Aya-ay, MD, Resident Nasal Reconstruction Following Electrical Injury: A Case Report Melanie Aya-ay, MD, Susan Rainey ARNP, Tampa General Regional Burn Center,Melissa Coombs PA-C, Tampa General Regional Burn Center, C. Wayne Cruse, MD, USF College of Medicine, Division of Plastic Surgery, Associate Director of Tampa General Regional Burn Center, David Smith Jr., MD, USF College of Medicine, Division of Plastic Surgery, Director of Tampa General Regional Burn Center Keywords: nasal reconstruction, nasal fistula, electrical injury Objective: Electrical injuries represent only a small percentage of burn admissions each year. Facial involvement is not typically seen. Nasal injuries involving skin, fat, cartilage, and mucosa are very uncommon and may result in a nasal fistula. Methods: A 72 year old male presented to our burn center with a full thickness burn to the nose after he lost consciousness while working on a 220V air conditioning unit. Following serial excisions of the nasal full thickness burn injury, a split thickness sheet graft was placed. Cartilage was exposed centrally in the defect, therefore, graft survival in this area was tenuous. POD #16 the patient returned to clinic with mucous drainage and airflow through the central aspect of the skin graft. A nasal fistula had developed. Approximately two and a half months after the initial electrical injury, the patient underwent delay of a dorsal turnover flap. Three weeks later the dorsal turnover flap was lifted to form the nasal lining while a forehead flap was used to reconstruct the overlying skin and soft tissue. Division and insetting of the flap concluded the reconstruction. Results: A three-stage reconstruction of the nasal fistula resulted in an acceptable aesthetic and functional outcome. The patient’s symptoms of mucous drainage and air flow via the fistula resolved. There were no postoperative complications noted. Conclusion: Severe tissue damage to the nose is an uncommon finding in electrical injuries. When irreparable damage affects all of the layers of the nose, a nasal fistula may occur. Serial excisions may be indicated to better define the extent of injury and to attempt to preserve tissue. Staged surgical procedures often maximize aesthetic and functional outcomes.

Abstract #: B-84 Presented by: Vinil Badri, MD, Staff Spontaneous Autorosette Formation In The Peripheral Blood Of ALS Patients Vinil Badri, MD,COM, USF,Islam A. Saleh1, Theresa Zesiewicz3, Yong Xie1, Kelly L. Sullivan3, Christopher L. Ward3, Amber M. Miller3, Md Shahaduzzaman1, Paul R. Sanberg 1,2,4,5,6, and Svitlana N. Garbuzova-Davis1,2,4,5, 1Center of Excellence for Aging & Brain Repair, Departments of 2Neurosurgery, 3Neurology, 4Molecular Pharmacology and Physiology, 5Pathology and Cell Biology, and 6Psychiatry, USF, COM, Department of Neurosurgery Keywords: ALS Patients,Autorosette,Immune system. Objective: Amyotrophic lateral sclerosis (ALS) is a motor neuron disease without identifiable markers of disease progression. Increasing evidence points to immune system involvement in ALS. Significant lymphopenia has been noted in ALS patients even at early stage disease and, accompanied by spontaneous rosette formation with autologous erythrocytes, was also found in mice modeling ALS (Garbuzova-Davis et al. 2005).The aim of this study was to evaluate autorosette formation in the peripheral blood of ALS patients. Methods: Blood was obtained from 12 ALS patients and 5 healthy volunteers. Complete blood counts (CBCs) were performed. Blood smears were stained with Giemsa and examined under a light microscope. Images were taken from various areas of each slide and the percentage of E-rosette calculated. Immunohistochemical staining for CD2 was also performed. Results: Eight ALS patients (Group 1, ALSRS 15-25) had E-rosette formation with lymphocytes (3.2±1.37) and neutrophils (10.5±1.53). Four ALS patients (Group 2, ALSRS 26-35) had E-rosette formation with lymphocytes (1.0±0.67) and neutrophils (7.8±2.16). In five healthy volunteers (ALSRS 48), there was no E-rosette formation with lymphocytes and only two had neutrophil E-rosettes of 1.3% and 2.2%. CD2+ cells were found in Group 1 (2.1±0.85), Group 2 (1.1±0.34), and healthy volunteers (0.2±0.27). CBCs were correlated with number of autorosettes in patients. Conclusion: Results show that ALS patients differ in lymphocyte functionality, possibly due to differences in immune system response. Patients most severely affected (Group 1) showed increased spontaneous autorosette formation with lymphocytes and neutrophils. Autorosette formation may be useful as a biological marker for the progression of ALS. Research supported by: Department

Abstract #: B-85 Presented by: Andrew Bagg, MD, Resident Systemic Reactions To Percutaneous And Intradermal Skin Tests Andrew Bagg,Chacko T, Glaum M, Ledford D, Fox R, Lockey RF. USF COM, Dept. of Medicine, Division of Allergy/Immunology, Dept. of Medicine, Division of Allergy/Immunology, Department of Internal Medicine Keywords: skin test reactions Objective: The purpose of this study is to determine over 12 months, 2/1/06-1/31/07, the rate of Systemic reactions(SRs) to both percutaneous(P) and intradermal(ID) skin tests(ST), the symptoms reported, and the response to immediate treatment with epinephrine IM. Methods: A retrospective review over a one year period was conducted to evaluate SRs to P and ID ST to 20 to 50 allergens (trees, grasses, weeds, animals, molds, foods,medications, and Hymenoptera) in 1,456 subjects. Nurses as instructed by the attending physicians administered epinephrine 1:1000 v/v, 0.2mL IM as soon as any signs or symptoms of anaphylaxis occurred. Results: 52 patients (3.5%) had SRs, 43 (83%) female and 9 (17%)male. The average age of the patients with SRs was 40.6 years (range 13-70, median 35.5 years). 17/52 (33%) had asthma. Symptoms reported: pruritic eyes, nose, and/or pharynx (40%), worsening cough (27%), sensation of difficulty swallowing (17%), worsening nasal congestion (15%), rhinorrhea (13%), chest tightness and/or shortness of breath (13%), generalized pruritus (12%), sneezing (12%), urticaria (4%), and wheeze (4%). No severe asthma, shock, hypotension, unconsciousness, or late phase responses occurred. Treatment: 52 (100%) patients received epinephrine (average dose, 0.2 cc, 1:1000 IM), 48 (92%) oralprednisone, 9 (17%) oral prednisone to take 6 to 8 hours after reaction, 50 (96%) oral antihistamine (H1) , and 6 (12%) nebulized beta agonist. Conclusion: SRs occurred in 3.5% of patients skin tested and readily responded to early intervention with epinephrine. This early administration of epinephrine by nurses appears to prevent more serious and late phase reactions.

Abstract #: B-86 Presented by: Aliyah Baluch, MD, Resident Career Choice of USF Medical Graduates Compared to National Averages from 1999 to 2007 Aliyah Baluch,Ashley E. Sponaugle, B.A. Ren Chen, MD, MPH Sateesh Prakash, MD USF COM, Department of Internal Medicine Keywords: USF COM, NRMP, Residency Match Objective: Post graduate career choices are an important aspect to the ongoing evolution of medical education. Our study was a comparison of University of South Florida’s medical graduates from 1999 to 2007 to evaluate for a similarity in specialty choice and to illustrate the trend in specialty selection. Methods: Career fields were placed into seven categories; internal medicine (IM), family practice, pediatrics, obstetrics-gynecology, psychiatry, surgery, and specialties/other. Data were collected from the National Resident Matching Program statistics and the residency match results from the USF College of Medicine. Results: Within USF from 1999 to 2007, graduates showed an increase in entering specialties/other, decrease in entering IM, and entrance to all other categories remained constant. Compared to national averages, there was a significant difference in IM choice (t value: 14.81, p value < 0.0001), and specialties/other choice (t value: 1-9.31, p value < 0.0001). A comparison between choice in primary care versus specialties showed there was a significant difference (t-value: 10.79, p-value <0.0001) as well. Opposite the national trend, USF medical graduates chose to enter specialties more often than primary care. Conclusion: Understanding differing trends among individual medical schools may give insight into the unique characteristics of medical training offered at individual medical schools such as the University of South Florida.

Abstract #: B-87 Presented by: John Branch, MD, Resident Sequence Of Tonometry And Pachymetry In The Ophthalmic Examination John Branch, MD, Christine Callahan M.D. USF, Jackie Scott USF USF COM, Department of Ophthalmology Keywords: pachymetry, tonometry, ophthalmology Objective: To determine if the sequence of tonometry and pachymetry testing has an affect on the readings. Methods: This was a randomized, prospective study examining forty-three patients and normal volunteers from Bascom Palmer and University of South Florida, who presented to the glaucoma clinic for routine evaluation. Patients that suffered from eye disease that affected the accuracy of tonometry or pachymetry readings were excluded. After informed consent was obtained, subjects were randomized into two groups, either initial pachymetry, or initial tonometry measurement of the right eye. One eye was measured in the sequence pachymetry-tonometry-pachymetry. The fellow eye was measured in the sequence tonometry-pachymetry-tonometry. A single examiner, utilizing the Goldman Applanation Tonometer and the PachetteÓ Ultrasound Pachymeter, sequentially performed the measurements. Less than one minute elapsed between measurements. Patient demographic data, diagnosis, and ocular medications were recorded. Results: There is only negligible effect of tonometry on pachymetry and slightly more in the reverse Conclusion: Tonometry should be done before pachymetry in the ophthalmic exam. Research supported by: USF department of Ophthalmology

Abstract #: B-88 Presented by: Natalie Brown, MD, Resident Alopecia Universalis Preceeding Development of Dermatomyositis. Natalie Brown, MD, USF COM Division of Rheumatology Frank Vasey MD. COM USF, Department of Internal Medicine, Division of Rheumatology Keywords: Dermatomyositis Alopecia Universalis Silicone Implants Objective: We describe a case of dermatomyositis preceded by the development of alopecia universalis Methods: A 51 year old white female with a past medical history significant for hypothyroidism, vitiligo, idiopathic peripheral eosinophilia since childhood, psoriasis, and ruptured silicone breast implants developed alopecia universialis, a nonscarring alopecia affecting her scalp, eyebrows, eyelashes, axilla, and pubic area. The alopecia failed to respond to steroid injections to the scalp and protopic ointment. An ANA screen was initially positive, then negative. She was diagnosed with possible early lupus and treated with hydroxychloriquine and low dose prednisone. She later developed a sudden onset erythemetous rash to her scalp, upper arms, face, chest, back, and shoulders with Gottron’s papules. She experienced difficulty lifting her hands up and standing up from a chair. Her ANA titer was 1:1250. All other antibodies, including jo-1 antibody, were negative. A skin biopsy of the upper back was positive for interface dermatitis which was consistent clinically with dermatomyositis. The rash responded well to forty milligrams daily of prednisone and she developed hair re-growth on her scalp, eyebrows, eyelashes, axilla, and pubic area. Her muscle strength and fatigue also improved. Results: This is the first report in the literature of alopecia universalis presenting prior to onset of or in association with dermatomyositis Dermatomyositis has been reported in the literature in association with ruptured silicone breast implants. It could be possible that alopecia universalis and dermatomyositis share similar immunopathology. Conclusion: Alopecia universalis is a rare precursor to dermatomyositis.

Abstract #: B-89 Presented by: Edisson Cantor, Athletic Training Student, Undergraduate Body Mass Index for FIFA World Cup Professional Soccer Players Edisson Cantor,,Jeff Konin, PhD, ATC, PT Department of Orthopaedics & Sports Medicine University of South Florida Keywords: body mass index soccer FIFA Objective: The objective of this research was to identify body mass index trends of world cup soccer players. Our previous work has documented body mass index for University of South Florida, Big East Conference, and Major League Soccer body mass index. Methods: Height and weights were recorded for all players listed on rosters for the 32 teams participating in the 2006 FIFA World Cup. This information was publicly available through FIFA websites. Using standardized and internationally accepted formulas, body mass was calculated by position for each team as well as for the entire league. Results: Team body mass index ranged from 82.6% - 100% considered to have normal body mass. Team findings of overweight players averaged from 0% - 12%. Few teams had players who were underweight according to standardized classification systems. Overall for the league, the following BMI was recorded: underweight 1%, normal 92%, overwight 6%, obese 1%. By position for the league, goalkeepers were found to be 85% normal, with 14% overweight, defenders were 93% normal and 6% overweight, midfielders 94% normal and 5% overweight, and forwards, 92% normal and 5% overweight. Very few overall were classified as either being obese or underweight. Conclusion: The findings of this research reflect similar patterns previously found with college aged and professional soccer athletes. Continued research needs to be performed to assess the validity of BMI in athletes.

Abstract #: B-90 Presented by: Nicole Draper, MD, Resident Dispermic Chimerism Identified During HLA Typing For Stem Cell Transplantation. Nicole L. Draper* Cheryl Conley† Clayton Smith‡ Kaaron Benson* *Department of Pathology H. Lee Moffitt Cancer Center, Tampa †Orchid GeneScreen Dayton, Ohio ‡Leukemia/BMT Program of British Columbia Vancouver Hospital & Health Sciences Centre BC Cancer Agency and University of BC Vancouver, British Columbia, Canada USF COM Basic Biomedical Sciences - Pathology and Cell Biology Keywords: chimerism, HLA typing, STR typing Objective: Chimerism is defined as the presence of two genetically distinct cell populations in an organism. Few cases of phenotypically normal dispermic chimeras have been reported. Herein we describe a phenotypically normal man with normal blood typing in whom dispermic chimerism was suspected after HLA typing. Methods: Case report. Results: A 32-year-old man was diagnosed with AML. He clearly typed as group A, Rh-negative. No abnormalities of sexual development were identified on multiple physical exams, previous exploratory surgery or CT scans. Molecular HLA typing (sequence-specific primers) in preparation for stem cell transplant showed the patient to have three HLA-B* and three HLA-Cw* alleles. Initial serologic HLA typing reported two haplotypes, but on subsequent review reactions for a third HLA-B antigen that were initially deemed to be false positive reactions were identified. Two of ten microsatellite short tandem repeat (STR) loci also showed three distinct alleles in blood and buccal samples. In all studies the third allele was attributable to a dual paternal contribution. Conclusion: This case represents dispermic chimerism, with one maternal and two paternal haplotypes variably distributed throughout body tissues in a phenotypically normal man without abnormalities in blood typing. The presence of additional alleles that may have been undetected or dismissed by serologic typing should be carefully investigated and verified by molecular techniques. Molecular HLA typing may increase the accurate identification of phenotypically normal chimeras, and aid in selecting proper donors for transplantation to reduce graft-versus-host disease and transplant rejection in these patients.

Abstract #: B-91 Presented by: Jonathan Dreier, MD, Resident Femoral Nerve Catheter Infusion vs. IV PCA of Fentanyl in Knee Replacement Surgery: A Local Analgesic Phenomenon. J. Dreier 1, R. Karlnoski 2, E. Benton 1,2, E.M. Camporesi 1,2, D. Mangar 1,2 1Department of Anesthesiology and Critical Care Medicine, USF COM 2Florida Gulf-to-Bay Anesthesiology Associates, Tampa, Florida Keywords: postoperative pain , opioids, fentanyl, analgesia, femoral nerve blockade Objective: Postoperative pain after total-knee replacement (TKR) is a major concern. We designed the present study to compare efficacy of pain management among three femoral nerve sheath (FNS) catheter infusates: fentanyl alone, ropivacaine alone and fentanyl and ropivacaine in combination. Methods: Seventy patients who underwent TKR under standardized general anesthesia participated in this study. A FNS catheter was placed pre-operatively. Post-operatively, a randomly assigned infusion of fentanyl (3µg/ml), ropivacaine (0.1%), a combination of fentanyl and ropivacaine (3µg/ml + 0.1%), or saline was initiated through the catheter. An additional group of patients were placed on an intravenous fentanyl PCA pump to correlate serum fentanyl levels with level of analgesia. Pain was assessed postoperatively using the VAS scores. Bilateral knee strength was determined using hand-held dynamometry. Plasma fentanyl levels and an evaluation of morphine use were obtained. Results: Serum fentanyl levels were significantly lower when infused via the FNS catheter compared to the IV PCA group. Post-operative analgesia using the FNS catheter infused with fentanyl was comparable to that of ropivacaine-containing infusates, all requiring significantly less morphine than the control saline infusion. VAS scores were not different between the groups. Postoperative strength was significantly greater in the fentanyl only group compared to the other treatment groups. Conclusion: These findings suggest that the analgesic effect reported is a local phenomenon rather than an overall systemic effect. The benefit achieved from catheter administration of fentanyl shows promise in providing analgesia while facilitating greater recovery and muscle strength. Research supported by: Funding from Stryker Instruments

Abstract #: B-92 Presented by: Paul Edwards, M.D, Resident

Non Invasive Extendable Prostheses: Report on the Only Cohort of American Patients Edwards, Paul M.D, German A. Marulanda, M.D.1, Eric Henderson, M.D.1, Nazeem Virani, M.D.1, David Cheong, M.D.2, and G. Douglas Letson, M.D.2 1Department of Orthopaedics and Sports Medicine, USF COM. 2Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL

Keywords: Extendable prostheses, osteosarcoma, limb lengthening

Objective: The purpose of this study is to report on the short-term results of non-invasive extendable prostheses for the treatment of osteosarcoma in the only American series of patients. Of note, less than forty prostheses of this type have been implanted world-wide. Methods: From December 2004, to August 2007, 7 patients with osteosarcoma were treated by the senior author with a non-invasive extendable prosthesis. The device uses electromagnetic impulses to activate an embedded mechanism that results in controlled lengthening of the prosthesis without the need of additional surgical procedures. Patients were followed for a mean of 23 months (range, 4 to 36 months). Variables such as the number and extent of lengthenings, number of complications related to the use of the device, range of motion loss, the need for revision, radiographic analysis, and overall impact in survival will be assessed. Results: Five boys and two girls with a mean age of 9 years (range, 7 to 11 years of age) underwent 21 sequential limb lengthenings. One millimeter of expansion was achieved in every four minute session. The anatomic location of the device was the distal femur in five cases, the proximal femur in one case and the proximal tibia in the remaining patient. All of the patients had excellent function of the extremity defined as no postoperative loss of motion during the subsequent expansions. Conclusion: Extendable prostheses constitute a viable alternative of limb salvaging for children with osteosarcoma. The authors believe that non-invasive lengthening mechanisms obviate the morbidity associated with conventional extendable prostheses. Long term follow-up is needed to study long-term impact in survival, overall function, and patient/family satisfaction.

Abstract #: B-93 Presented by: David Estores, MD, Faculty Chronic Esophagitis Dissecans Presenting with Dysphagia and Esophageal Strictures David Estores, MD Jane L. Messina, MD and Domenico Coppola, MD -Department of Pathology, USF COM, H. Worth Boyce, MD - Department of Internal Medicine, USF COM, Department of Internal Medicine Keywords: esophagitis dissecans esophageal disorders dysphagia esophagitis Objective: Chronic esophagitis dissecans superficialis (CED) is a rare condition characterized by sloughing of esophageal mucosa, dysphagia and and certain microscopic (immunohistochemical & histopathologic) features. There are less than 20 cases of CEDS described in the medical literature. At the USF Center for Esophageal and swallowing Disorders (USF-CESD) we present 2 cases of CED diagnosed in 1999 and 2006. Methods: We identified 2 cases of CED at the USF-CESD (out of more than 6,500 patients). Results: Both patients share common features: both are females (ages 78 and 67 years); a chronic history of dysphagia spanning 5 and 7 years before diagnosis; no history of caustic substance ingestion nor of any systemic autoimmune disease; and complicated strictures present on endoscopy. One patient had no symptomatic response to dilation therapy. Endoscopic appearance were typical of CED with erythema and friability of the esophageal mucosa and mucosal sloughing. Biopsy specimens contained soft fragments of esophageal mucosa which on light microscopy shows detached fragments of squamous epithelium. There were some inflammatory cells seen. Eosinophils are noted to be rare (<1/hpf). There were no microorganisms or evidence of viral infection. An immunohistochemical stain for ICAM-1 was strongly positive in specimens from one patient. Immunofluorescence tests for the detection of IgM and IgG were negative. Conclusion: The entity of CED is a rare form of chronic esophagitis with characteristic endoscopic and microscopic features which should be considered in the differential diagnosis of chronic dysphagia.

Abstract #: B-94 Presented by: Joel Hunter, MD, Resident Photorefractive Keratectomy in Eyes with Topographic Pellucid Marginal Degeneration or Forme Fruste Keratoconus Joel Hunter, MD,Lewis Groden M.D. USF COM Department of Ophthalmology Keywords: topography, keratoconus, pellucid, refractive, PRK Objective: To measure refractive outcomes in PRK performed on eyes with subclinical keratoconus or pellucid marginal degeneration. Methods: Retrospective study of 120 eyes split into two groups of 60. Group 1 with normal topography and group 2 with abnormal topography. PRK was performed on both groups and post-op visual acuity was evaluated. Results: There was no difference between refractive outcome in PRK performed on eyes with normal vs. abnormal topography. Conclusion: PRK provides excellent visual acuity results for patients with normal topography and subclinical keratoconus and subclinical pellucid marginal degeneration.

Abstract #: B-95 Presented by: Sunir Joshi, MD, Resident Logarithmic Versus Non-logarithmic Behavior of the Quadrant Sum in Humphrey V-Spot Visual Fields S. Joshi, D. Richards, P. Kamath. Ophthalmology, University of South Florida, Tampa, FL. USF COM, Department of Anesthesiology Keywords: glaucoma, visual fields, ophthalmology Objective: The Zeiss-Humphrey V-spot automated threshold visual field test (“V-spot”) is frequently used clinically for patients with advanced field loss and/or decreased visual acuity. We compared the behavior of the V-spot quadrant sum (QS) in the logarithmic (db, geometric) versus non-logarithmic (arithmetic) domains. Methods: A total of twenty-seven Fastpac and Full Threshold 24-2 V-spot tests of seven eyes of five patients were transferred to a Sun Workstation. Range of QS was 0 to 419 db. The individual db sensitivities for the 108 quadrants were analyzed, using Interactive Data Language software, to produce the arithmetic mean (AM) and geometric mean (GM) for each quadrant. Mathematically, log(GM) = QS / 10 / (number of points in the quadrant). Standard deviations of the original db values and of their arithmetic equivalents were also computed for each quadrant. Arithmetic values were converted to db for comparison purposes. Results: For 4 ranges of QS (0-100, 101-200, 201-300, and >300), average AM (GM) were, in db: 7.8(3.3), 18.9(11.5), 24.1(18.9), 28.4(26.5). Average standard deviations for the same intervals were: 5.5(4.6), 6.9(9.0), 4.7(5.2), 2.8(5.2). Conclusion: The QS (or its equivalent, GM) consistently underestimates the average sensitivity of the quadrant for all ranges of QS. Standard deviations computed in the logarithmic domain are sometimes significantly greater than in the arithmetic domain. QS may not be the ideal estimator of average loss per quadrant in V-spot tests. Research supported by: University of South Florida

Abstract #: B-96 Presented by: Christine Kay, MD, Resident Optical Coherence Tomography Findings in Retinal Phototoxicity Christine Kay, MD, Dr. Peter Reed Pavan (USF Ophthalmology) Dr. Amy Zaccaria Martino (USF PGY-1) USF COM, Department of Ophthalmology Keywords: phototoxicity, macula, retina, neurosensory detachment, OCT Objective: This case reports the Optical Coherence Tomography (OCT) findings in operating light microscope induced retinal phototoxicity, documenting a neurosensory detachment associated with decreased vision after phototoxic insult. Methods: This is a case report. This report presents OCT documentation of a neurosensory detachment in patient with a history, fundus findings, and fluorescein angiogram typical for operating light microscope induced retinal phototoxicity. Results: Vision on post-operative day (POD) 1 was 20/70 OD with a normal appearing macula. On POD 13, vision OD had decreased to 20/200. Fundus examination revealed a subretinal horizontal pigment line in the inferior macula. Fluorescein angiography revealed early staining and late leakage in the area of RPE atrophy with blockage of underlying choroidal fluorescence by the hyperpigmented border and inferior pigment line OCT on POD 13 revealed a large, central neurosensory detachment with a central thickness of 731 microns. Visual acuity was 20/200. BCVA OD on POD 31 had improved to 20/60 and the OCT had minimal subretinal fluid with a central thickness of 226 microns. On POD 78, there was no subretinal fluid, vision was 20/50, and the central thickness was 191 microns. Four months post-operatively, BCVA was 20/30. Conclusion: The presence of a neurosensory detachment on OCT has not been previously reported in microscope light induced retinal phototoxicity. The horizontal line in the inferior macula may be an indication of severe phototoxicity.

Abstract #: B-97 Presented by: Jeff Konin, PhD, Faculty Body Mass Index In Men’s Collegiate Athletics Compared Between University And Conference Teams Jeff Konin, PhD, Kenzo Koike USF COM, Department of Orthopaedic Surgery Keywords: body mass index athletes big east Objective: Body mass index is a numeric measure that many health professionals use as an indicator of obesity. In this study, the body mass index (BMI) of specific men’s athletics was compared between the University of South Florida (USF) and 15 universities collectively in the Big East Conference (BEC). Methods: Height and weight was gathered from team rosters for the 2007 seasons of football (BEC N=746, USF N=101), soccer (BEC N=388, USF N=22), baseball (BEC N=324, USF N=40), and basketball (BEC N=203, USF N=13) provided by the respective universities and the BEC. Results: Average BMI data was calculated for each sport and categorized by team and players’ positions. The average BMI’s calculated for BEC sports were 29.5 (football, +/- 4.17), 23.5 (soccer, +/- 1.46), 25.4 (baseball, +/- 2.21), and 24.8 (basketball, +/- 2.05). Average BMI’s calculated for USF sports were 29.6 (football, +/- 4.62), 22.6 (soccer, +/- 1.07), 25.8 (baseball, +/- 2.44), and 23.9 (basketball, +/- 2.03). With the exception of USF place-kickers, the calculated BMI data categorized all football positions in the BEC and USF as overweight (BMI of 25.0-29.9). Defensive and offensive linemen were additionally categorized as obese (BMI > 30.0). Calculated BMI’s of all soccer positions in the BEC and USF were categorized as normal (BMI of 18.5-24.9), while all baseball positions in the BEC and USF were categorized as overweight. Centers and forwards in BEC basketball were categorized as overweight while only the center (n=1) at USF was considered overweight. Conclusion: . In all, the BMI’s of USF men’s athletes compared similarly with BMI’s of the BEC, but this study also highlights that BMI may not be an adequate indicator of sports fitness in collegiate athletics.

Abstract #: B-98 Presented by: Stephen Kucera, MD, Resident Frequency Of Distal Esophageal Landmark Identification And Adherence To Biopsy Standards At Index Endoscopy In Patients Referred For Ablation Therapy For Barrett’s Esophagus Stephen Kucera, MD, Sabo Tanimu, MD (H. Lee Moffitt Cancer Center), Jason Klapman, MD (H. Lee Moffitt Cancer Center), James Barthel, MD (H. Lee Moffitt Cancer Center) USF COM, Department of Internal Medicine Keywords: Barrett's Esophagus, Quality, Endoscopy Objective: To evaluate the frequency of distal esophageal landmark identification and adequacy of mucosal biopsy sampling at index endoscopy (IE) from referring gastroenterologists. Methods: A retrospective review of 49 patients treated with PDT and Barrx for BE with HGD between 10/15/03 and 10/15/07. IE reports were reviewed for identification of the following esophageal landmarks 1)DH, 2)GEJ, and 3)SQJ. The number of biopsies taken from the segment of BE at each IE was recorded. An IE report was considered complete if all 3 esophageal landmarks were identified and adequate if both the GEJ and SQJ were identified. The IE was considered to contain an appropriate number of biopsies if 4 quadrant biopsies were obtained every 2 cm of the Barrett’s segment. Results: 13/49 (26.5%) IE reports were complete and 25/49 (51%) were adequate. 14/49 (28.6%) IE contained an appropriate number of biopsies for the length of BE. 7/26 cases (26.9%) of long segment BE (> 3 cm) IE contained appropriate biopsy sampling. 14/23 cases (60.9%) of short segment BE (< 3 cm), IE contained appropriate biopsy sampling. The average length and number of biopsies taken at IE for long segment BE was 6.8 cm and 9.6 biopsies compared to 1.70 cm and 3.4 biopsies for short segment BE. Contingency table analysis using Fisher’s Exact Test revealed P=0.022 for comparison of biopsy sampling between short and long segment BE. Conclusion: The percentage of IE reports which were complete or adequate, and the percentage containing appropriate numbers of biopsies for the length of BE were low. We also observed a statistically significant difference between short and long segment BE in regards to adequacy of biopsy sampling.

Abstract #: B-99 Presented by: Jennifer Leigh, MPH, Med IV Student-Watson Clinic An Operational Cost Comparison Of Ambulatory Endoscopy Cost In Southeast U.S. vs. National Benchmarks Jennifer Leigh, MPH,USF,COM, Minh Ngo, B.S.,USF, COM, Sateesh R. Prakash, M.D., USF Department of Internal Medicine David Estores, M.D.,USF Swallow Center Jay R. Prakash, M.D., South Atlanta Medical Clinic, USF, COM, Department of Internal Medicine Keywords: Cost, Ambulatory Center, Southeast, GI Objective: Ambulatory surgical centers (ASC) have become a popular alternative for outpatient procedures, especially in the field of gastroenterology (GI). The possibility of lower operational expenditure offers a benefit for patients, physicians, and insurance companies. Currently, there is sparse information comparing southeast ASC costs with national benchmark standards. Our goal is to compare the annual operational costs of a single specialty ASC in the southeast United States to national benchmark standards. Methods: We selected G.I. Endoscopy Center (GIEC), a single specialty ASC in Georgia. Standard operational costs, defined as telephone, laundry, biomedical engineering, and housekeeping costs, were compared to similar 2006 national benchmarks from Olympus America, Inc for ASC performing 0-3,000 proceedures/yr. Cost per case, annual standard operational cost and percent savings were calculated. Results: Total standard operational cost for GIEC was $5.11/case and $15,330/year. National benchmark data revealed standard operational costs of $12.12/case and $36,360/year. The cost difference is $7.01/case and $21,030/year representing a 57.84% reduction in standard operational costs in the southeast region. Conclusion: Our study revealed a lower standard operational cost for the single specialty GI ASC in the southeast region compared to national benchmark costs for 2006. The greatest cost savings was found in the biomedical engineering costs. Though our data suggests a significant difference in standard operational costs, efficiency can vary among centers in the same geographic region. Our study emphasizes the importance of improving ASC efficiency in an effort to reduce national healthcare costs in the United States.

Abstract #: B-100 Presented by: Kaiwen Lin, BS, Med IV Student-Watson Clinic Errors in EEG Interpretation And The Misdiagnosis Of Epilepsy: Which Patterns Are Over-Read? Kaiwen Lin, BS,,Selim R. Benbadis, M.D..Professor and Director Comprehensive Epilepsy Program at USF Departments of Neurology and Neurosurgery, USF, COM, Department of Neurology Keywords: epilepsy, diagnosis, misdiagnosis, EEG Objective: The over-interpretation of EEGs is common and is an important contributor to the misdiagnosis of epilepsy. We reviewed our experience in order to clarify which EEG patterns are commonly over-read as epileptiform. Methods: We identified patients who were seen at our epilepsy clinic and were ultimately diagnosed with conditions other than epilepsy. We selected those who had previously had an EEG read as showing epileptiform discharges, and whose EEG was available for our own re-review. Results: 37 patients met the above criteria. Eventual diagnoses were psychogenic nonepileptic seizures (10), syncope (7), other miscellaneous diagnoses (5), and unexplained nonspecific symptoms (15). None of the EEGs had epileptiform discharges. The descriptions of the abnormalities included "temporal sharp waves" in 30, "frontal sharp waves" in 2, and "generalized spike-wave complexes" in 2. Three had no reports available to identify the alleged abnormality. The benign patterns mistaken for temporal (30) and frontal (2) sharp waves were simple fluctuations of background activity with temporal phase reversals. Conclusion: By far the most common patterns over-read as epileptiform are non-specific fluctuations of background in the temporal regions misread as temporal sharp waves.

Abstract #: B-101 Presented by: German Marulanda, MD, Resident Judet’s Quadricepsplasty for Treatment of Knee Extension Contracture After Prolonged External Fixation German Marulanda, MD,Odion Binitie, M.D, Department of Orthopaedics and Sports Medicine, USF COM, George Haidukewych, M.D., Adult Reconstruction and Trauma, Florida Orthopaedic Institute Michael A. Mont, M.D., Center for Joint Preservation and Reconstruction, Rubin Institute for Advanced Orthopedics, Sinai Hospital of Baltimore G. Douglas Letson, M.D.,Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute Keywords: Knee Extension Contracture Arthrofibrosis Judet Quadricepsplasty Objective: Long term immobilization of the lower extremity is associated to extensor mechanism dysfunction and the development of a stiff knee joint. The Judet quadricepsplasty is one method to treat this event. The purpose of this study was to describe a modified surgical technique and evaluate the patient satisfaction and clinical and radiographic outcome of stiff knees treated with this procedure. Methods: From June, 2000 to January, 2005, 39 patients with stiff knees secondary to prolonged external fixation were treated utilizing the modified Judet quadricepsplasty technique. There were 20 men and 19 women with a mean age of 34 years (range, 14 to 54 years). The mean length of arthrodesis of the knee was 12 months (range, 5 to 18 months). Clinical and radiographic outcome were evaluated pre- and post-operatively. Patients were followed for at least 2 years, mean 3, (range, 2 to 5 years). Results: The mean post-operative knee flexion was 80°, (range, 5 to 95 degrees). The mean preoperative Knee society score was 35 points (range, 10 to 55 points). The mean post-operative score was 80 points (range, 18 to 95 points). Two patients presented a 10º and 15º loss of terminal active extension, respectively. Ninety five percent of patients were able to ambulate without aid after the procedure. Two patients did not achieve adequate range of motion despite a structured post-operative physical therapy protocol. Conclusion: The Judet quadricepsplasty is an acceptable alternative to treat patients with contracture of the knee secondary to severe trauma and long-term external fixation. Although it is a technically demanding procedure, the biomechanical rationale in which the technique is based represents a logical sequence to address the effect of prolonged fixation.

Abstract #: B-102 Presented by: Connor Morton, BS, Staff Is Survival After Pancreatectomy For Adenocarcinoma Impacted By Performance Status Connor Morton, BS,, John Mullinax MD, Sam Al-Saadi MD, Desiree Villadolid MPH, Michael Vogt MD, Sarah Cowgill MD, Steven Goldin MD, Alexander S. Rosemurgy MD, USF, COM, Division of General Surgery, Department of Surgery Keywords: pancreatic cancer, adenocarcinoma, KPS, survival Objective: While many factors impact survival after pancreatectomy, intuitively, patients with the best performance status have the best prognosis. This study was undertaken to determine the independent impact of performance status after pancreatectomy on survival. Methods: Patients with a Karnofsky Performance Status (KPS)>60 after pancreatectomy for adenocarcinoma were enrolled into a placebo-controlled prospective randomized adjuvant therapy trial. For 96 patients randomized to receive placebo, the impact of KPS at 6 weeks after pancreatectomy on survival was determined. Patients were followed for 24 months after the last patient was enrolled into the trial. Results: Resections were R0 for 66% and R1 for 33% of the patients. AJCC Stage was IA for 9%, IB for 21%, IIA for 11%, and IIB for 56% of the patients. Overall survival was a median of 12 months, with 35% of patients alive at 24 months. KPS after pancreatectomy did not correlate with survival by regression analysis or by survival curve analyses when patients were stratified by KPS. Conclusion: For patients with adenocarcinoma undergoing pancreatectomy without adjuvant therapy, survival is poor. While, intuitively, healthiest patients have the best prospects for prolonged survival, performance status (i.e., KPS ³ 60) after pancreatectomy is not related to and does not independently impact survival. Since performance status does not impact survival after pancreatectomy for adenocarcinoma and since patients undergoing pancreatectomy for adenocarcinoma generally succumb to recurrent cancer, performance status seems not to impact the biological behavior of pancreatic cancer following resection.

Abstract #: B-103 Presented by: Helen Moses, BS, Med IV Student-Watson Clinic Fabry Cardiomyopathy: A Story Of Mother And Son Helen Moses, BS,USF, COM,Dr. Jean Go MD, USF Internal Medicine, Dr.Michael Yarnoz MD , Dr.Maya Guglin MD PhD FACC,USF Assistant Professor of Medicine, Director Heart Failure.USF, COM, Department of Internal Medicine Keywords: Fabry disease,?-galactosidase A- deficiency,lysosomal storage disorder, Enzyme replacement therapy, Cardiomyopathy Objective: Fabry Disease (FD) is an X-linked metabolic disorder that results in a deficiency of lysosomal enzyme ?-glactosidase A. FD leads to accumulation of glycosphingolipids in endothelial and smooth muscle cells of renal and cardiovascular systems. Female carriers are not as severely affected as males. Methods: Case Report Results: 65 yo female with FD has hypertension, chest pain, and non-obstructive coronary artery disease. 2DE showed EF of 50%, mild concentric left ventricular hypertrophy, global hypokineses, and diastolic dysfunction. Her son was diagnosed with FD at age 19, with earlier presenting symptoms of acroparesthesias and hypohydrosis. Renal insufficieny progressed despite enzyme replacement. He was put on chronic hemodialysis in his 4th decade. He also suffered from multiple thrombotic events and avascular hip necrosis. He was admitted at age 44 for worsening dyspnea. Troponin was mildly elevated, but coronary angiography revealed normal arteries. His cardiac chambers were mildly-moderately dilated; EF was 15%. An AICD was implanted, and the patient began standard heart failure treatment. At age 45, he experienced frequent episodes of ventricular tachycardia terminated by shocks from his defibrillator. Repeated catheterization demonstrated rapid progression of coronary disease which required coronary artery bypass surgery. The patient was rejected for heart/kidney transplant due to systemic involvement. He died of electrical storm after the defibrillator was turned off due to futility of care. Conclusion: Typical Fabry cardiomyopathy involves only left ventricular hypertrophy. This case demonstrates an uncommon spectrum of cardiac manifestations of FD, from benign manifestations in mother to fatal illness in son.

Abstract #: B-104 Presented by: Tapan Padhya, MD, Faculty A Cost Analysis of the PEG with Annual Enteral Feeding Tapan Padhya, MD, Sateesh R. Prakash,MD, Kathleen Sullivan,RD, Teresa DeLeo,MSW USF COM Department of Otolaryngology Keywords: Cost Analysis, PEG, Annual Objective: Percutaneous endoscopic gastrostomy (PEG) and enteral feeding have evolved to become a popular modality for nutrition. The goal of our study was to evaluate the cost of PEG placement and feeding annually at Moffitt Center. Methods: We defined the cost of PEG feeding to equal the cost of the procedure and the annual costs of enteral feeds in patiets with head and neck cancer. All costs were based on 2007 Medicare Part B Rates for Nestle Nutrition Products with nutritional needs of 2200 calories/day. We created 4 categories of patients (A,B,C,D) and evaluated each category with the selected method of feeding. Supply costs were then calculated and selected methods of feeding were bolus, gravity, and pump. Nursing costs were then calculated as the mean nursing cost. The approximate cost for the PEG placement procedure was estimated which included the following: professional fee, facility fee, and miscellaneous items. Professional fee was defined as the medicare reimbursement for the gastroenterologist, anesthesiologist, and CRNA for their services. Facility Fee includes the cost of performing the procedure at MCC. Results: The annual cost ranged from $7,558.72 to $24,519.52. The least expensive cost is for patient A with Bolus feeding and the most expensive cost is for patient D with pump feeding. Since patients in our population were completely dependent on PEG feeds for nutrition, the net cost was calculated as the difference in the total PEG associated cost and the cost that an individual would otherwise spend on food for daily nutrition. Conclusion: The difference ranged from $3,942.72 to $19,718.40. Despite the benefits, physicians should consider the increased costs in evaluating patients for PEG for an extended duration of time.

Abstract #: B-105 Presented by: Marie Pierre, MD, Resident Gastroschisis in the State of Florida, 1998-2003: Assessing the Risk Factor Profile Marie Pierre, MD, Jennifer L Kornosky, MSPH1, Jason L Salemi, MPH1, Kimberlea W Hauser, MBA1 and Jane D Carver, PhD, MS1.1Pediatrics, USF COM Department of Pediatrics Keywords: epidemiology, gastroschisis Objective: Identify risk factors that are associated with the increasing prevalence of gastroschisis in Florida Methods: Data obtained from the Florida Birth Defects Registry included infants born between January 1, 1998 and December 31, 2003. Covariates in the logistic regression model included maternal age, race, maternal country of birth, parity, and infant sex. Results: Cases: There were 1,216,142 live births in Florida during the study period and 397 gastroschisis cases (prevalence rate: 3.3/10,000 live births) Maternal Age: Compared with the 25 to 29 age group (referent), the odds ratio for delivering an infant with gastroschisis was 5.8 (95% CI 4.4-7.8) for women less than 20 years old and 2.4 (1.8-3.2) for women 20-24. Women over 30 were significantly less likely to deliver an infant with gastroschisis. Ethnicity: Hispanic women were less likely than non-Hispanic women to deliver an infant with gastroschisis. After adjusting for maternal country of birth, maternal age and parity, the association between Hispanic ethnicity and risk of gastroschisis was eliminated: OR 1.02, 95% CI 0.8-1.3. Maternal Country of Origin: Regardless of ethnicity, prevalence rates for gastroschisis were lower for foreign-born women . Conclusion: Our data confirm that young maternal age is a significant risk factor for gastroschisis. The relationship between ethnicity and risk of gastroschisis is complex; maternal country of birth appears to be an important covariate. While a significant limitation of our study is a lack of information regarding the length of time that women resided in their country of birth, our data suggest that unidentified factors related to residence in the U.S. may increase the risk of gastroschisis.

Abstract #: B-106 Presented by: Sateesh Prakash, MD, Resident An Overview of the Inlet Patch Sateesh Prakash, MD,David S. Estores,Jr.,M.D., Katheryne Downes,M.P.H., Matthew Hatler, H.Worth Boyce,M.D. USF COM, Department of Internal Medicine Keywords: Inlet Patch; Esophagus; Gastroenterology; Swallowing Objective: The “Inlet Patch” (IP) is ectopic gastric mucosa located in the proximal esophagus. There are limited data regarding IP and its clinical significance. Many gastroenterologists are unaware of IP. Most find IP incidentally during endoscopy. However, this finding may have a significant clinical impact. The goal of our study is to evaluate the relationship between the inlet patch and various conditions in the upper GI tract. Methods: Our study reviewed data from patients seen at the USF Center for Esophageal and Swallowing Disorders over 20 years with IP and without IP. The conditions include: squamous cell carcinoma, esophageal web, Barrett Esophagus (BE), NSAID exposure, pill-induced esophagitis, eosinophilic esophagitis, duodenal ulcer, gastric ulcer, reflux stricture, perforations, adenocarcinoma, neoplasm, dysplasia, respiratory symptoms, dysphagia, polyps, globus sensation, esophageal spasm, and achalasia. Statistical analysis was employed to evaluate any association between IP and other conditions. Results: In descending order we found an association between IP and the following: esophageal web; BE; strictures; adenocarcinoma; and dysplasia. We found no association between IP and the following: achalasia; esophageal spasm; globus sensation; dysphagia; respiratory symptoms; pharynx/larynx neoplasm; perforations; stricture; and gastric ulcer. We were unable to evaluate an association between IP and squamous cell carcinoma, NSAID exposure, pill-induced esophagitis, duodenal ulcer, and non-reflux strictures. Conclusion: There appears to be a strong relationship between IP and conditions related to increased esophageal acid exposure. This phenomenon could be explained by the production of acid by metaplastic gastric epithelium.

Abstract #: B-107 Presented by: David W Richards, MD, Faculty Non-Logarithmic Analysis of Automated Visual Field Tests David W Richards, MD, Michael H. Rotberg, MD (A) Priyanka Kamath, MS (B) A. Charlotte Eye Ear Nose and Throat Associates, Charlotte, North Carolina B.Department of Ophthalmology, USF USF COM Department of Ophthalmology Keywords: Glaucoma, Visual Fields, Logarithmic Objective: To compare the mathematical behavior of spatial averages of automated visual field tests in the decibel (logarithmic, geometric) and linear (non-logarithmic, arithmetic) domains. Methods: Ninety-six Zeiss-Humphrey Sita-Standard III-Spot 24-2 automated visual field tests (of 72 eyes of 44 patients) were re-analyzed in the linear domain. Spatial averages of the entire field (mean defect, “MD”) and standard errors of MD ( pattern standard deviation, “PSD”) were compared with the traditional values, as calculated in the decibel domain. Results: The values of MD in the decibel domain are almost always an underestimate of the linear values, and the underestimation becomes worse as MD decreases. In addition, MD values in the decibel domain may exhibit significant scatter for similar linear values. Values of PSD in the decibel domain, when compared with their linear equivalents, become inaccurate for small values of MD. Conclusion: The traditional calculation of MD and PSD in the decibel domain leads to disparity with the alternative method of spatial averaging in the linear domain, and the disparity increases as visual field defects worsen. Any spatial averaging of visual fields will be subject to these effects, whose precise clinical and research significance remain to be determined. Research supported by: Internal Grant USF College of Medicine

Abstract #: B-108 Presented by: Samantha Roland, BA, Med IV Student-Watson Clinic The effect of Non-Logarithmic Analysis On Point Wise Linear Regression (Plr) Of Automated Visual Fields S.Roland,USF, COM, P.Kameth, J.Carter, D.Richards, USF, COM. Department of Ophthalmology Keywords: Visual Fields Point Wise Regression Objective: PLR is a commonly used method to detect change of automated visual fields (AVF). To date, PLR has used decibel values from AVFs as input. We have investigated the effect of using arithmetic equivalents of decibel values in the linear regression process. Methods: Zeiss Humphrey Sita Standard 24-2 AVFs for 10 patients were analyzed. There were 8-11 serial AVFs extending over 5-10 years for each patient. Total deviation values for each field were converted to arithmetic equivalents using Interactive Data Language software on a Sun workstation. Linear regression was carried out in both the decibel and arithmetic domains and the results were compared. Results: A total of 520 points (10 patients by 52 points/patient) were analyzed by comparing best-fit slopes in the two domains. Slopes differed by at least ± 0.5 db/y in 12.1% of cases and by at least ± 1.0 db/y in 4.2% of cases, and were as great at 3 db year. Clusters of slope differences occurred adjacent to areas of field loss in some cases. Conclusion: PLR in the db domain can alter conclusions about pointwise progression of AVFs. PLR should preferentially be performed in the arithmetic domain. Research supported by: USF Ophthalmology

Abstract #: B-109 Presented by: Rene Ruiz, MD, Resident Analysis of Foxp3 Protein Expression in Term and Preterm Infant Cord Blood CD4+CD25+ T cells Rene Ruiz, M.D., Matthew Morrow, M.S., Morna Dorsey, M.D., M.M.Sc., John Sleasman, M.D., Department of Pediatrics, USF COM Keywords: Foxp3 Cord Blood CD4+CD25+ T cells Objective: To measure TREG cell percentages in cord blood and to relate those values to demographic data and gestational age. Methods: In this on-going study, umbilical cord blood is obtained from infants born at 24 to 42 weeks gestational age and mononuclear cells are isolated. Extracellular (CD4 and CD25) and intracytoplasmic (Foxp3) staining are performed, and CD4+CD25+Foxp3+ T cells are identified and enumerated by flow cytometry. Results: Preliminary data from 10 infants between 35 and 40 weeks gestation reveal a mean percentage of TREG cells of 5.9 +/- 0.9%. There is a significant negative correlation between TREG percentages and gestational age (r2=0.58, p=0.01). Conclusion: These data indicate that the percentages of TREG cells in fetal blood decrease with advancing gestational age, and support findings from animal studies that TREG cells may play an important role in maintaining feto-maternal tolerance. On-going studies will determine if TREG levels relate to clinical outcomes in pre-term infants.

Abstract #: B-110 Presented by: Dawn Schocken, MPH, Faculty Simulation Used in End of Life Issues Dawn Schocken, MPH,,Nicole Murphy,Center for Advanced Clinical Learning, USF Health; Brad Peckler, Center for Advanced Clinical Learning, USF Health; Bryan Bognar, Office of Educational Affairs, USF Health, COM Department of Internal Medicine Keywords: Simulation, Medical Education, End-of-Life Issues Objective: Hi-Fidelity simulation is rapidly becoming accepted as an advanced teaching tool in all stages of practice. We sought to use simulation in an end of year high stakes Clinical Performance Examination (CPX) where MS IIIs were asked to make critical action decisions. Students were faced with an end of life scenario where they were asked to discuss management decisions with family members of a traumatic terminal patient. Methods: All students had prior exposure to simulators during their educational year. The patient was a simulator who was a 28 year old victim of a gunshot wound to the head, CT showed the bullet crossing midline. Notes from the Trauma and Neurosurgical service indicated the patient was non-operative and brain dead. Trained Standardized Patients (SPs) were the family with the patient. The student was asked to write a progress note. Results: A total of 117 students took this exam. 96% of these students discussed organ donation, while 84% mentioned advanced directives and/or power of attorney. 100% of the students committed to death as the eventual outcome. The results for the issues regarding end of life care and complications were mixed. Conclusion: Third year medical students receive no formal training in this point of their career to deal with end of life issues. While their exposure is touched upon, it is only as an ancillary member of the team. Upon examining student responses, the team recommends the inclusion of medical students in the discussions surrounding end of life issues and traumatic terminal illness in their academic training.

Abstract #: B-111 Presented by: Neil Sharma, MD, Resident An Elderly Male with Intermittent Abdominal Pain: An Atypical Case of Intussusception Neil Sharma, M.D. (Chief resident, USF Department of Internal Medicine, pgyIV) Jeffrey Kooper, M.D. (VA hosptialist, USF Assistant Professor of Medicine) Andrew Koon, M.D. ((VA hosptialist, USF Assistant Professor of Medicine) USF COM, Department of Internal Medicine Keywords: intussusception abdominal pain gastroenterology medicine Objective: Intussusception is usually a disease of infancy with 80% presenting by 2 yrs of age. Adults account for ~5-10% of all reported cases. We present a case of an 81 year old male with an uncommon etiology of abdominal pain: ileal-ileal intussusception captured on inpatient admission with striking CT scan images. Methods: A case report of a patient cared for by USF Department of Internal Medicine. The case outlines the entire hospital course of a patient admitted for intermittent colicky abdominal pain. Results: An 81 y/o Caucasian male presents to the emergency department with abdominal pain for two days. The patient describes the pain as “sharp,” periumbilical in location, non-radiating, lasting approximately 1 to 5 minutes, and occurring several times throughout the day. The patient notes a decreased appetite secondary to increased pain and nausea with eating. Laboratory findings are significant only for a mildly elevated white blood cell count. A CT scan of the abdomen and pelvis with contrast obtained in the emergency room reveals a two centimeter ileal-ileal intussusception in the right abdomen without small bowel obstruction or visualized mass. (Please refer to pictures at time of publication.) He had a benign hospital course. Conclusion: Discussion about intussusception in adults including prevelance, etiologies, and treatment.

Abstract #: B-112 Presented by: Byol Shin, DO, Resident A Woman with Dry Cough and Progressive Dyspnea Byol Shin, Dr. Dennis Ledford Professor of Medicine, Allergy and Clinical Immunology USF COM, Department of Internal Medicine Keywords: Hypersensitivity pneumonitis Vocal cord dysfunction Objective: Hypersensitivity pneumonitis (HP), also known as extrinsic allergic alveolitis, is an inflammatory interstitial lung disease, caused by inhaled antigens that elicit lymphocytic inflammation in the peripheral airways and surrounding interstitial tissue. Concurrent presentation of HP and vocal cord dysfunction (VCD) is unusual. Methods: This is a case report of a patient who presented with a dry cough and progressive dyspnea as the clinical manifestations of HP to African grey parrot and VCD. Results: A previously healthy 48 year-old female referred to an allergy and immunology clinic for the evaluation of a dry cough and progressive dyspnea 12 months earlier. The physical exam showed diffuse end-inspiratory wheezes and crackles. Pulmonary function testing (PFT) showed a restrictive defect, decreased DLCO, and flattening inspiratory loop. Rhinolaryngoscopy was negative for VCD, but subsequent videostroboscopy showed evidence for VCD. The radiological studies revealed diffuse ground-glass opacities without significant fibrosis or lymphadenopathy. Cell counts in bronchoalveolar fluid showed 77% lymphocytes with a CD4:CD8 ratio of 3.2. Transbronchial biopsies revealed lymphocytic interstitial infiltration without granulomas. Bacterial, viral, fungal and mycobacterial studies were all negative. The laboratory studies showed the presence of precipitating antibodies against parrot antigen. Elimination of parrot and corticosteroid therapy led to improvement of her symptoms. Conclusion: Diagnosis of HP is often difficult or misdiagnosed as asthma as with the current case. Dry cough and progressive dyspnea have a broad differential diagnosis and can present as the combination of HP and VCD.

Abstract #: B-113 Presented by: Paul Toomey, BS, Med IV Student-Watson Clinic Survival After Pancreaticoduodenectomy Is Not Improved By Extending Resections To Achieve Negative Margins Paul Toomey, BS,USF, COM, Jonathan Hernandez MD, Desiree Villadolid MPH, John Mullinax MD, C Whalen Clark MD, Lorraine Tomlinson, Steven Goldin MD PhD, Alexander Rosemurgy MD USF, COM, Department of Surgery Keywords: Survival, pancreaticoduodenectomy, margins, pancreatic cancer Objective: Resection remains the only hope of cure for pancreatic adenocarcinoma. This study was undertaken to determine the survival benefit, if any, of extending resections to obtain microscopically negative margins after positive intra-operative frozen sections. Methods: Since 1995, patients undergoing pancreaticoduodenectomy for pancreatic adenocarcinoma are followed prospectively. Margin status has been codified as micro- / macroscopically negative (R0) or microscopically positive / macroscopically negative (R1). The impact of margin status on survival was evaluated utilizing survival curve analysis. Results: Of 179 patients undergoing pancreaticoduodenectomy for pancreatic adenocarcinoma, complete tumor extirpation (R0) was initially achieved in 134 patients, was ultimately achieved in 17 patients who required extended pancreatic resections after initially positive intra-operative frozen sections (R1toR0), and not achieved in 28 patients (R1). No correlation between AJCC Stage and margin status was identified by regression analysis. Patients undergoing R0 resections lived longer than patients undergoing R1 resections. Survival was not improved if the pancreatic resections were extended to obtain negative (R1toR0) margins. Conclusion: Although resection remains the only hope for cure for patients with pancreatic cancer, even with resection survival is disappointing. Patients undergoing less than complete tumor extirpation have particularly poor survival. Survival after pancreaticoduodenectomy is not improved by extending resections to achieve negative margins after positive intra-operative frozen sections, suggesting the importance of “tumor specific” factors (e.g., infiltrating nature, tumor immunobiology) relative to margin status.

Abstract #: B-114 Presented by: Joanne Valeriano, MD, Faculty Joint Injection Training Model Joanne Valeriano, MD, Jason Guthrie, M.D. Rheumatology Fellow Ashley, Sterrett, M.D. Rheumatology Fellow Natalie Brown, M.D. Rheumatology Fellow USF COM Department of Internal Medicine Keywords: Knee shoulder arthrocentesis injection model Objective: Assess the knowledge of knee and shoulder injection procedures prior and after a formal workshop developed by USF Department of Rheumatology for internal medicine residents. Methods: Internal medicine residents at USF are asked to participate in a knee and shoulder injection workshop. Prior to the workshop each resident is asked to evaluate his/her level of confidence in performing injections of these joints. The workshop entails a full anatomical review of the knee and shoulder using both a PowerPoint presentation developed by USF rheumatology and an injection simulator. Indications, contradictions, and dosages are reviewed. Proper technique is demonstrated and then residents are allowed to practice using a simulator that gives auditory feedback (saw bones). After completion of the workshop residents are then asked to reevaluate their level of confidence performing these procedures. Participating residents will then be followed-up regarding utilization and confidence in performing knee and shoulder injections post-workshop. Results: The average comfort levels improved in all residents post workshop. Conclusion: Preliminary results suggest the workshop improved the residents comfort level in performing knee and shoulder examination and arthrocentesis.