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مدت زمان جوابدهی ره آزمایش رد آزمااگشیه پاتوبیولوژی و مولکولی فرزان

No: 13& 15 , Nili Alley, Buali Sidewalk, Hamedan Tel: 08132534465

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بخش هماتولوژیTest sample

sample volume(m

l) Method Stability

interferences/Reject/Preparation befor

sampling جوابدهی

جوابدهی اورژانسی

Refrence value

CBC Diff - Whole

blood(EDTA) 1

Cell counter(Flow

cytometry)

4 hrs at RT 1 day at 2-8 C

Hemolysis/Frozen/Clotting/incorrect anti

coagulant 1 day 2hrs *

ESR Whole

blood(Sodium citrat)

2 Automation 4 hrs at RT 12 hrs at 2-8 C

Hemolysis/Clotting 1 day 2hrs Women: 1-20 Men: 1-15

Blood group & RH (DU)

Whole blood(EDTA)

1.5 hemagglutation 1 day at RT 1weeks at 2-8 C

Hemolysis/Frozen/Clotting

1 day 2hrs *

Pt (Prothrombin

time)

Plasma(Sodium citrat)

2 Coagulation 2 hrs at RT 2weeks at -20

Hemolysis/ Notice Use of warfarin,coumadin, heroin/Be separated

quickly

1 day 2hrs 10-14 Second

LE cell Blood 10 * The same time must be tested

Not Recommended 1 day 2hrs *

Retic count Whole

blood(EDTA) 1.5 Supravital stian

6 hrs at RT 3 days at 2-8 C

Hemolysis/Frozen/Clotting/incorrect anti

coagulant 1 day 2hrs

Adults: 0.5-1.5 % childs: 2-6 %





Fluid Analyse Body fluid 1 * The same time must be tested

Not Recommended 1 day 2hrs *

Ptt Plasma(Sodium

citrat) 1 Coagulation

2 hrs at RT 4 hrs at 2-8 C 2weeks at -20

Hemolysis/ Notice Use of Argatroban,

Heparin, heroin/Be separated quickly

1 day 2hrs 25-35 Second

Bleedig Time(BT)

* * Duke * Avoid Using

nticoagulant drugs 1 day 2hrs 1-4 Min

Clotting Time (CT)

* * Tube method * Avoid Using

nticoagulant drugs 1 day 2hrs 4-8 Min





خون بخش بیوشیمی

Test sample sample volume

(ml) Method Stability




Refrence value

Glucose

Serum,Plasma(EDTA,Heparin Oxalat, fluoride)

0.3 Photometric 5 days at 2-8 C 6 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle

1day 2 hrs

infants: Cord blood: 63-158 mg/dl 1h: 36-99 mg/dl 2h: 36-89 mg/dl 5-14 h:34-77 mg/dl 10-28h: 46-81 mg/dl 44-52h:48-79 mg/dl Childs (Fasting): 1-6 Years: 74-127 mg/dl 7-19 Years: 70-106 mg/dl Adults (Fasting): 70-115 mg/dl

Bun Serum,heparinized plasma

0.3 Photometric 7 days at 4-25 C 1 year at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle, elevated ammonia/Nephrotoxic drugs will change blood urea level

1day 2 hrs

Childs: 1-3 Years: 11- 36 mg/dl 4-13 Years:15-36 mg/dl 14-19 Years: 18-45 mg/dl Women: 20-50 Years: 15-40 mg/dl > 50 Years: 21 - 43 mg/dl Men: 20-50 Years: 19-44 mg/dl > 50 Years: 18-55 mg/dl





Creatinine

Serum,Plasma(EDTA,Heparin, Oxalat, fluoride)

0.3 JAFFE 7 days at 2-8 C 3 Months at -20

High concentration of reducing compound/Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Avoid using if nephrotoxic drugs

1day 2 hrs

Women: 0.6-1.1 mg/dl Men: 09-1.3 mg/dl

Uric Acid


0.3 URICASE/PEROXIDASE

7 days at 2-8 C 3 Months at -20 C

Hemolysis, Icteric & Lipemic serum/avoid repeated freez/thow cycle/Abstain using salicylates befor few days

1day 2 hrs

Women:2.6-6 mg/dl Men: 3.5 -7.2 mg/dl

Cholesterol



Hemolysis, Icteric & Lipemic serum/avoid repeated freez/thow cycle/ Fasting is recommended

1day 2 hrs

Normal: < 200 mg/dl Borderline:200-240mg/dl High: > 240 mg/dl

Triglyserid


0.3 Photometric 5 days at 2-8 C 3 Months at -20

Hemolysis, Icteric serum//Avoid repeated freez/thow cycle/Abstain using alchol befor 3 days

1day 2 hrs

Normal: < 200 mg/dl Borderline: 200-400 mg/dl High: > 400 mg/dl





HDL cholestrol

Serum,Plasma(EDTA,Lithiu

m, Sodium heparin)

0.3 Photometric 4 days at 4-6C 2weeks at -20 C(Dark place)

Fasting/Avoid using clofibrate 7 days prior sample collection/Hemolysis, Icteric & Lipemic serum

1day 2 hrs

childs And adults: =>35 mg/dl

LDL cholestrol

Serum,Plasma(ED

TA, Sodium heparin)

0.3 Photometric 5 days at 2-8 C 1 month at -20 C(Dark place)

Fasting/Hemolysis, Icteric & Lipemic serum/Avoid repeated freez/thow cycle

1day 2 hrs

Normal: < = 130 mg/dl Borderline: 130-160 mg/dl High: > 160 mg/dl

Ph Serum,heparinized plasma

0.3 phosphomolyb

date.UV 4 days at 2-8 C 3 Months at -20 C

Fasting/Hemolysis, Icteric & Lipemic serum/Avoid repeated freez/thow cycle

1day 2 hrs

childs: 4-7 mg/dl Adults:2.5-5 mg/dl

AST

SerumPlasma(EDTA,heparin

)


Hemolysis, Lipemia/Notice use of hepatotoxic drug/avoid repeated freez/thow cycle

1day 2 hrs

Women: < 31 U/l Men: < 37 U/l





ALT


)


Hemolysis, Lipemia/Notice use of hepatotoxic drug/avoid repeated freez/thow cycle

1day 2 hrs

Women: < 31 U/l Men: < 41 U/l

AlKaline phosphatas

e

Serum,heparinized plasma

0.3 Photometric 1 weeks at 4-8 C 2 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle, Notice use of hepatotoxic drug

1day 2 hrs

childs up to 15 Years: 180-1200 Men: 80-306 IU/L Women: 64-306 IU/L

Direct Bilirubin


0.3 Photometric

1 days at 20-25 C 7 days at 2-8 C 3 Months at -20 C( ا درصورتیکه سریع Dark)(فریز شودPlace)

Hemolysis, Lipemic/avoid repeated freez/thow cycle, Notice use of hepatotoxic drug

1day 2 hrs

childs And adults: =<0.3 mg/dl

Total Bilirubin


0.3 Photometric

1 days at 20-25 C 7 days at 2-8 C 3 Months at -20 C( ا درصورتیکه سریع Dark)(فریز شودPlace)

Hemolysis, Lipemic/avoid repeated freez/thow cycle, Notice use of hepatotoxic drug

1day 2 hrs

Infants: 1 day: < 8.8 mg/dl 2 days:1.3-11.3 mg/dl 3 days: 0.7-12.7 mg/dl 4-6 days:0.1-12.6 mg/dl Childs < 1 Months:0.2-1mg/dl Adults: 0.1-1.2 mg/dl





Iron Serum,heparinized plasma

0.3 Photometric 4 days at 15-25 C 7 days at 2-8 C 3 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Abstain iron compound 7 days prior to collection and OCP/Sampling should be done preferably at 8-10 am

1day 2 hrs

childs: 1-14 Days: 63-201mg/dl 15 Days-6 M: 28-135 7M-1 yrs: 35-155 2yrs-12yrs: 22-135 women: 12yrs-25yrs: 37-165 25yrs-40yrs: 23-134 40yrs-60yrs: 39-149 >60 yrs : 55-150 12 weeks beginning of pregnancy: 42-177 Late pregnancy: 25-137 Up to 6 weeks after accouchement: 16-150 Men: 12yrs-25yrs: 40-155 25yrs-40yrs: 35-168 40yrs-60yrs: 40-120 >60 yrs : 55-150

TIBC Serum 0.3 colorimetry 1 days at 15-30 C 4 days at 2-8 C 3 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/ Fasting

1day 2 hrs 250-450 ug/dl

Amylase


)



1day 2 hrs < 100 U/l





CPK


)

0.3 Photometric

1 days at 15-25 C 7 days at 2-8 C 1 Months at -20 C (Dark Place)

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Avoid heavy physical activity prior test

1day 2 hrs

Women: 24-170 U/l Men:24-195 U/l infants (New born):468-1200 U/l infants 1-5day :195-700 U/l infants up to 6 Months:41-330 U/l Children> 6 Months: 24-229 U/l

Gamma GT

Serum,Plasma(ED

TA) 0.3 Photometric


Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/ Fasting is recommended/Avoid using OCP/

1day 2 hrs Women: < 32 U/l Men: < 49 U/l

CRP quantitative

Serum 0.3 immunoturbidi

metry

5 days at 20-25 C 1 Month at 2-8 C 1 year at -20 C

Hemolysis, Icteric & Lipemic serum

1day 2 hrs

Adults:Up to 5 mg/L

Total Protein


0.3 Spectrophoto

metric

6 days at 15-20 C 1 Month at 2-8 C 1 year at -20 C

Hemolysis, Icteric & Lipemic serum/avoid repeated freez/thow cycle

1day 2 hrs 6.4-8.3 g/dl





Ca Serum,heparinized plasma

0.3 Photometric 7 days at 20-25 C 3 weeks at 4-8 C 8 Months at -20 C

Hemolysis, Lipemic & icteric serum/Fasting is recommended/avoid repeated freez/thow cycle/ Blood collection without tourniquet/

1day 2 hrs 8.6-10.3 mg/dl

Zinc Serum,heparinized plasma

0.3 colorimetry 5 days at 2-8 C 3 Months at -20

Hemolysis, Lipemic & icteric serum

1day 2 hrs

Men: 72.6-127ug/dl women: 70-114 ug/dl children: 63.8-110 ug/dl New-born: 49.5-99.7 ug/dl

Magnesium Serum,heparinized plasma

0.3 XYLIDYL

BLUE 7 days at 2-8 C 3 Months at -20 C


1day 2 hrs 1.7-2.4 mg/dl

Albumin

Serum,Plasma(EDTA,heparin, citrate)



1day 2 hrs Adults: 3.5-5.2 g/dl

Amylase


)



1day 2 hrs < 100 U/l





Potassium Serum 0.3 ISE 7 days at 2-8 C 3 Months at -20 C

Blood collection without tourniquet/Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/

1day 2 hrs 3.5-5.5 mMol/l

sodium Serum 0.3 ISE 7 days at 2-8 C 3 Months at -20 C

Hemolysis 1day 2 hrs 135-145 mMol/l

Urin Protein

Urine(24 hrs,

Random ) * Pyrogallol-Red

Urine: 24 hrs at 2-8 C For longer time at -20 C Random Urin: Non jemolysis CSF: 72 hrs at 2-8C

Hemolysis CSF. 1day 2 hrs

10-150 mg/24h pregnant women(3-9 Months):10-255 mg/24h Random urine: < 14 mg/dl Adult CSF: 15-45 mg/dl Infant CSF:30-100 mg/ dl





شیمی اردار بخش

Test sample نگهدارنده Method Stability جوابدهی جوابدهی

اورژانسیRefrence value

Amylase Urine * Photometric 10 days at 4-8 C 3 weeksat -20 C 1day 2hrs Random: < 450 U/L 24hrs: <410 U/L

Magnesium Urine 10 ml Hcl 10% XYLIDYL BLUE 1 week at 2-8 C 1day 2hrs 72.9-122 mg/24hrs

Uric Acid Urine Boric acid URICASE/PEROXIDASE 4 days at RT if adjusment of ph > 7.0 1day 2hrs 250-750 mg/24hrs

Creatinine Urine Boric acid JAFFE 24 hrs at 2-8 C 1day 2hrs Men: 14-28 mg/Kg/24hrs Women: 11-20 mg/Kg/24hrs

Ph Urine 10 ml Hcl 10%,

Boric acid phosphomolybdate.U

V 7 days at 2-8 C 1day 2hrs

0.4-1.3 g/24h

Zink Urine 10 ml Hcl 6

mol/L Colorimetry 5 days at 2-8 C 3 Months at -20 1day 2hrs

24HRS: 300-800 ug/24hrs Random: 15-150 ug/dl

Ca Urine 10 ml Nitric acid

50% Photometric 4 days at 4-8 C 3 weeks at -20 C 1day 2hrs

Men: < 300 mg/24hrs Women:<250 mg/24hrs

Urea Urine Boric acid Photometric 7 days at 4-8 C 1 Month at -20 C 1day 2hrs 13-36 g/24 hrs





Sodium Urine Boric acid ISE 7 days at 2-8 C 3 Months at -20 C 1day 2hrs 75-200 meq/24hrs

Potassium Urine Boric acid ISE 7 days at 2-8 C 3 Months at -20 C 1day 2hrs 40-80 meq/24hrs

Micro Protein Urine Boric acid Pyrogallol-Red Urine: 24 hrs at 2-8 C For longer time at -20 C Random Urin:

1day 2hrs

10-150 mg/24h pregnant women(3-9 Months):10-255 mg/24h Random urine: < 14 mg/dl

بخش نفلومتری



interferences/Reject/Preparation befor sampling

جوابدهیجوابدهی اورژانسی

Refrence value

Anti CCP Serum 0.3 Nephlometery 4 days at 2-8 C Hemolysis, Lipemic & icteric serum/contaminated sample

1 day 2 hrs <= 30 IU/ml





Hb A1C Whole

blood(EDTA) 0.3 Nephlometery 7 days at 2-8 C

Hemolysis/Frozen/Clotting/incorrect anti coagulant

1 day 2 hrs 4.2%-6.5%

Micro-albuminuria

Urine 0.3 Nephlometery 3 days at 2-8 C For a longer Time at -20 C

avoid repeated freez/thow cycle/

1 day 2 hrs < = 25 mg/L

C3 Serum,

Plasma(EDTA, Heparin)

0.3 Nephlometery 1 day at 2-8 C 1 month at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated

freez/thow cycle 1 day 2 hrs 0.8-1.85 g/L

C4 Serum,




freez/thow cycle 1 day 2 hrs 0.1 - 0.4 g/L

NGAL Plasma(EDTA) 0.3 Nephlometery same day * 1 day 2 hrs 37 -180 ng/ml

IgA Serum,




freez/thow cycle 1 day 2 hrs 0.7-4 g/L





IgG Serum,




freez/thow cycle 1 day 2 hrs 7-16g/L

HS CRP Serum, Whole

blood 0.3 Nephlometery

7 day at 2-8 C 3 Months at -20


freez/thow cycle 1 day 2 hrs <=3 mg/L

ASO Serum, Plasma( Heparin)



freez/thow cycle 1 day 2 hrs 0-170 IU/ml

Retinol Binding

Protein(RBP) Serum 0.3 Nephlometery 1 day at 2-8 C


freez/thow cycle 1 day 2 hrs 25 - 70 mg/ L

Serum Amyloid

Protein A (SAA)

Serum,Plasma,Whole Blood

0.3 Nephlometery 3 day at 2-8 C Hemolysis, Lipemic & icteric

serum/avoid repeated freez/thow cycle

1 day 2 hrs < 10 mg/L

RF Quantitative

Serum, Plasma( Heparin)



1 day 2 hrs 0-20 IU/ml

B2-microglobin

Serum,Urine 0.3 Nephlometery 3 day at 2-8 C For longer Time at -20 C


freez/thow cycle 1 day 2 hrs

Serum: 1-3 mg/L Urine:0.1-0.3 mg/L





Cystatin C Serum, Plasma( Heparin)



1 day 2 hrs

Male:mg/L 3- 59 Years: 0.49 -1.14 60-88 Years: 0.71-1.63 Female:mg/L 3- 59 Years: 0.51-0.97 60-88 Years:0.62 - 1.54

IgM Serum 0.3 Nephlometery 2 day at 2-8 C otherwise freeze at -20 C


freez/thow cycle 1 day 2 hrs 0.4-2.3 g/L





بخش میکروبشناسیTest sample

sample volume(ml)

Method Stability interferences/Reject/Preparati

on befor sampling جوابدهی اورژانسی جوابدهی

Refrence value

H.pylori stool Antigen Stool 1 gram immunochromatogra

phic assay 2 days at 4-6C 1month at -20 C

Not recommended 1 day 1 hr qualitative

Stool occult blood Stool 1 gram Hema taype 1 hr at RT 1 days at 2-8 C 3 days at -20 C


FOB Stool 2 gram immunochromatogra

phic assay

2 hr at RT 1 days at 2-8 C 3 days at -20 C


Bence jones Protein Urin 10 Chemical 7 days at 4-6C 1month at -20 C






Blood culture Blood 10% of culture media

Aerobic & Anaerobic jar

At RT< 1hrs Avoid using antibiotic 10 days (3times

)

در صورت مثبت بودن،نتایج رنگ آمیزی گسترش و

directنتایج Smear همان

روزگزارش میشود

*

CSF culture CSF 1 Aerobic & Anaerobic

jar The same time must be tested

Abstain using antibiotic/ second tub sampling is

appropriate 4 days




*

Eye dis. Culture Eye dis. * Aerobic & Anaerobic


Avoid using antibiotic 4 days




*

Precardial fluid culture Precardial

fluid 5


The same time must be tested





*





Pleural fluid culture Pleural

fluid 5







*

semen culture Semen * Aerobic & Anaerobic






*

Stool culture Stool 1 gram Aerobic & Anaerobic

jar 2 hrs at RT 2 days at 2-8 C

Avoid using antibiotic/Reject frozen sample

3 days * *

Stool exam Stool 1 gram Aerobic & Anaerobic

jar

Bloody & mocusal sample the same time must be tested

Reject frozen sample,specimens containing

barium, oil, or urin 1 days 1 hr *

throat culture throat swab

* Aerobic & Anaerobic


Avoid using antibiotic 4 days * *





Urethral dis. Culture Urethral

dis. *




Urine culture Urine 10 Aerobic & Anaerobic



Urine Analysis Urine 10 Aerobic & Anaerobic


Not recommended 1 days 1 hr *

Vaginal culture Vaginal

dis. *




Wound culture Wound

dis. *








بخش ایمنواسی




جوابدهیجوابدهی


ANA Serum 0.3 IFA 2 days at 2-8 C Long-term storage at -20 C

Hemolysis, Icteric & Lipemic serum/Contaminated sample/avoid

repeated freez/thow cycle 1 week 3 days

Negative:< 80 Positive: => 80

Anti ds DNA Serum 0.3 IFA 2 days at 2-8 C Long-term storage at -20 C


repeated freez/thow cycle

1 week 3 days Negative:< 10 Positive: => 10





ANA complex Serum 0.3 IFA 3 days at 2-8 C Long-term storage at -20 C


repeated freez/thow cycle 1 week 3 days Qualitative

Systemic Sclerosis Prpfile

(IgG)

Serum, Plasma (EDTA,

Heparin, Citrate)

0.2 IFA 14 days at 2-8 C



Neurology Mosaics(IgG) (Autoimmune encephalitis)

Serum, Plasma (EDTA,

Heparin, Citrate),

CSF

0.2 IFA 14 days at 2-8 C







بخ ش هورمون شناسی

Test sample sample

volume(ml) Method Stability




Refrence value

HCV Ab Serum,

Plasma(Citrate,EDTA, Heparin)

0.4 Elisa 7 days at 2-8 C several Months at -20 C

Hemolysis, Lipemia,Residues of

fibrin,heavy particle,microbial body

& filament

3day 3 hrs Negative: < 0.9 Equivocal: 0.9-1.1 Positive: > 1.1

HIV Ab (1&2) Serum, Plasma 0.3 Elisa Hemolysis, Icteric &

Lipemic serum 3day 3 hrs Qualitative

HBC Ab Serum,





& filament


HAV Ab (Total) Serum,





& filament






HEV Ab Serum,





& filament


Vit D(25 OH) Serum 0.2 Elisa 2 days at 2-8 C 1 Months at -20 C

Hemolysis, Icteric & Lipemic serum/avoid repeated freez/thow

cycle

3day 3 hrs

deficient: <10 ng/ml Insufficent: 10-30 ng/ml Sufficient: 30-70 ng/ml More than Normal: 70-100 Intoxic: > 100 ng/ml

CH50 Serum 1 Elisa 2 hrs RT 1 Months at -20 C

Hemolysis/avoid repeated freez/thow

cycle 3 days 3 hrs

Absence or low: 0-50 % Normal: 51-150 % High: > 151 %

Prolactin Serum 0.4 Elisa 2 days at 2-8 C 14 days at -20 C

Hemolysis, Lipemic & icteric serum/Sample

collecting 3 hrs after 24 hrs after awake.

Abstain seual activity and stress 2 days befor

test, Avoid using estrogen, insulin… few

day befor test

3day 3 hrs Men: 1.8 -21.4 ng/ml Women:4.6- 29.5 ng/ml menopausal:1.5-18.5 ng/ml





بخش هورمون شناسی

Vidas sample sample volume





Refrence value

TSH Serum,Plasma (Heparin, Beads, Gel)

0.4 ELFA 5 days at 2-8 C

1Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow

cycle/Notice hormonal

&chemothrapy drugs

1day 2 hrs

Euthyroidism:0.3 - 5 uIU/ml

TSH Serum,Plasma (Heparin, Beads, Gel)

0.4 chemiluminescenc

e 5 days at 2-8 C

1Months at -20 C


cycle/Notice hormonal

&chemothrapy drugs

1day 2 hrs

0.42-5.45 uIU/ml





T3 Serum,Plasma (Heparin)

0.3 ELFA 1 hrs at RT

7days at 2-8 C 1Months at -20 C


cycle/Notice hormonal treatment

& gestation

1day 2 hrs

Euthyroidism: 0.92-2.33 nmol/l

T4 Serum,Plasma (Heparin)

0.4 ELFA 1 hrs at RT



cycle/Notice hormonal treatment

& gestation

1day 2 hrs

Euthyroidism: 60-120 nmol/l

Free T4 Serum,Plasma (Heparin, Silicon, Gel)


1 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Notice to drug interfernce such as

salicylates

1day 2 hrs

Euthyroidism: 10.89-20.30 pmol/l

Free T3 Serum,Plasma (Heparin)


1 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Notice to drug interfernce such as

salicylates

1day 2 hrs

Euthyroidism:4-8.3 pmol/l





Ferritin Serum,Plasma (Heparin,

EDTA) 0.3 ELFA

4 hrs at RT 2 days at 2-8 C

3 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Avoid using of iron 7 days prior to

collection

1day 2 hrs

men: 68-434 ng/ml women : cyclic 9.3-159 ng/ml menopausal: 24.4-278 ng/ml

BHCG Serum,Plasma (Heparin,

EDTA) 0.5 ELFA



cycle/Abstain using of chlorpromazine few days prior test(False

positive)

daily 2 hrs

men: < 3mIU/ml women: Cyclic < 4 menopausal: < 13

LH Serum,Plasma (Heparin)


3 Months at -20 C


cycle

1day 2 hrs

men: 1.1 - 7.0 mIU/ml women: ovulation (D 0 ): 9.6- 80.0 Folicular Ph: D -2 to - 8: 2.0 - 8.0 Folicular Ph: D -9 to - 15: 1.5 - 8.0 Luteal Ph: D +3 to +15: 0.2 - 6.5 menopausal: 8 - 33.0





FSH Serum,Plasma (Heparin)


3 Months at -20 C


cycle

1day 2 hrs

men: 1.7 - 12.0 mIU/ml women: ovulation (D 0 ): 6.3 - 24.0 Folicular Ph: D -2 to - 8: 2.9 - 9.0 Folicular Ph: D -9 to - 15: 3.9 - 12.0 Luteal Ph: D +3 to +15: 1.5 - 7.0 menopausal: 17 - 95.0

Testosteron Serum,Plasma (Heparin)


3 Months at -20 C

Hemolysis, Lipemic & icteric serum/the best time for sampling is 6-

10 am./ Fasting is recommended

1day 2 hrs

men: 2.27-10.30 female>=19-50 years : 0.23-0.73 female>50: 0.14-0.68

CEA Serum,Plasma (Heparin)


3 Months at -20 C


cycle/

1day 3 hrs

0.0 - 3.00 ng/ml





PSA Serum,Plasma (Heparin,

EDTA) 0.4 ELFA



cycle/Fasting is recommended/Absta

in prostate exam, heavy exercise & ejaculation 2 days

befor test

1day 3 hrs

Age : < 40Years: 0.21 - 1.72 ng/ml 40 - 49 years: 0.27 - 2.19 50 - 59 Years: 0.27 - 3.42 60 - 69 Years: 0.22 - 6.16 > 69 Years: 0.21 - 6.77

Free PSA Serum,Plasma (Heparin,

EDTA) 0.4 ELFA



1day 3 hrs

FPSA/TPSA:If>%18=>no biopsy necessary,make a follow up. FPSA/TPSA:If<%18=>make a biopsy,higher risk to be in front of acancer

Anti Tpo

Serum,Plasma (Heparin,

EDTA,Silicon, Gel)


3 Months at -20 C

Hemolysis, Lipemic & icteric serum/avoid repeated freez/thow cycle/Be separated

until 20-30 min

1day 2 hrs

<8 IU/ml negative >=8 IU/ml positive





Prolactin Serum,Plasma (Heparin)


3 Months at -20 C

Hemolysis, Lipemic & icteric serum/Sample collecting 3 hrs after 24 hrs after awake.

Abstain seual activity and stress 2 days

befor test, Avoid using estrogen, insulin… few day befor test

1day 2 hrs

Women: 5.0 - 35 ng/ml Men: 3.0 -25 ng/ml

AFP

Serum,Plasma (Heparin,

EDTA) Amniotic Fluid


3 Months at -20 C Hemolysis 1day 2 hrs

0-2 ul/ml

CA125 Serum,Plasma (Heparin,

EDTA) 0.4 ELFA



cycle/

1day 3 hrs

healthy subjects: 0.0 - 35 U/ml

CA19-9 Serum,Plasma (Heparin,

EDTA) 0.4 ELFA



cycle/

1day 3 hrs

healthy subjects: 0.0 - 37 U/ml





H.pylori IgG Serum,Plasm

a (EDTA) 0.3 ELFA



cycle/

1day 2 hrs

negative: TV < 0.75 equivocal: 0.75 ≤ TV < 1.00 positive: TV ≥ 1.00

HBS Ag Serum,Plasma (Heparin)

0.4 ELFA

8 hours at 18-25 C

1 days at 2-8 C several Months at

-20 C


fibrin, 1day 3 hrs

negative: index < 0.13 positive: index ≥ 0.13

Procalcitonin Serum,Plasma (Heparin)


3 Months at -20 C Hemolysis(gross) icteric serum

1day 2 hrs

No infection: < 0.1 Possible systemic infection: > = 0.1 and < 0.25 systemic infection (Sepsis likely): >= 0.25 and < 0.5 Sever sepsis or sepsis shock >= 0.5





Rubella IgG Serum,Plasma (Heparin,

EDTA) 0.5 ELFA



1day 2 hrs

negative: < 10 IU/ml equivocal: ≥ 10 to < 15 IU/ml positive: ≥ 15 IU/ml

Rubella IgM Serum 0.5 ELFA 7 days at 2-8 C

3 Months at -20 C Hemolysis, Lipemic &

icteric serum 1day 2 hrs

negative: < 0.8 equivocal: ≥ 0.8 to < 1.2 positive: ≥ 1.2

CMV IgM Serum 0.4 ELFA 7 days at 2-8 C

3 Months at -20 C


cycle/

1day 2 hrs

negative: < 0.7 equivocal: ≥ 0.7 to <0.9 positive: ≥ 0.9

Anti thyroglobulin

Serum,Plasma(Heparin,

EDTA,Silicon, Gel)


1 Months at -20 C


cycle/

1day 2 hrs < 18 IU/ml

Cortisol

Serum,Plasma (Heparin, EDTA)24 hour Urine


1 Months at -20 C


cycle/

1day 2 hrs

Morning: 60-285 ng/ml

afternoon: 40-150 ng/ml

urine: 20-110 Ug/ 24hrs





بخش هورمون شناسی






calprotectin Stool 1 gram Indirect

Elisa (Alegria)


Not applicable 1 day 3 hrs

Negative: < 50 μg/g Borderline: 50 - 200 μg/g Positive: > 200 μg/g

HSV Ab (IgG) Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 20 U/ml Borderline: 20 - 25 U/m Positive: > 25 U/ml





HSV Ab (IgM) Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 20 U/ml Borderline: 20 - 25 U/ml Positive: > 25 U/ml

Anti mitochondrial Ab(AMA)

Serum, Plasma(Citrate,EDTA,

Heparin) 0.2

Indirect Elisa

(Alegria)




Negative: < 10 IU/ml Positive: ≥ 10 IU/ml

Anti Cardiolipin Ab (IgM)


Heparin) 0.2

Indirect Elisa

(Alegria)




Negative: < 7 MPL-U/ml Positive: ≥ 7 MPL-U/ml

TTG Ab (IgG) Serum,


0.2 Indirect

Elisa (Alegria)





Anti-PR3 Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 5 U/ml Positive: ≥ 5 U/ml





Anti-MPO Serum,


0.2 Indirect

Elisa (Alegria)





Anti Phospholipid Ab (IgG)


Heparin) 0.2

Indirect Elisa

(Alegria)




Negative: < 10 GPL-U/ml Positive: ≥ 10 GPL-U/ml

Anti Phospholipid Ab (IgM)


Heparin) 0.2

Indirect Elisa

(Alegria)




Negative: < 10 MPL-U/ml Positive: ≥ 10 MPL-U/ml

ASCA IgA Serum,


0.2 Indirect

Elisa (Alegria)





Anti-MCV Serum,


0.2 Indirect

Elisa (Alegria)









Anti-SS-A 60 Serum,


0.2 Indirect

Elisa (Alegria)





Anti-LKM-1 Serum,


0.2 Indirect

Elisa (Alegria)





Anti-Centromere B Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 10 U/m Positive: ≥ 10 U/ml

Anti-Scl-70 (Topoisomerase)


Heparin) 0.2

Indirect Elisa

(Alegria)




Negative: < 15 U/ml Borderline: 15 - 25 U/mlPositive: > 25 U/ml

Anti-Lactoferrin Stool 0.2 Indirect

Elisa (Alegria)


Not applicable 1 day 3 hrs

Negative: < 10 U/m Positive: ≥ 10 U/ml





Anti-VZV IgG Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 50 mIU/ml Borderline: 50 - 100 mIU/ml Positive: > 100 mIU/ml

Anti-VZV IgM Abs Serum,


0.2 Indirect

Elisa (Alegria)




Negative: < 20 U/ml Borderline: 20-25 U/ml Positive: > 25 U/ml

Anti-Gliadin IgG Serum,


0.2 Indirect

Elisa (Alegria)



freez/thow cycle/ Fasting is recommended

1 day 3 hrs


Anti-EBV (VCA) IgG Serum,


0.2 Indirect

Elisa (Alegria)





Anti-beta-2-Glycoprotein I IgG


Heparin) 0.2

Indirect Elisa

(Alegria)





Anti-beta-2-Glycoprotein I IgM


Heparin) 0.2

Indirect Elisa

(Alegria)









Anti-SS-B Serum,


0.2 Indirect

Elisa (Alegria)





Anti-EBV (VCA) IgM Abs.


Heparin) 0.2

Indirect Elisa

(Alegria)









سرولوژی بخش

Test sample sample

volume(ml) Method Stability




RF Serum 0.2 Agglutination 7 days at 2-8 C 3 Months at -20

Hemolysis, Lipemia, Contamination 1 day 2 hrs Qualitative

CRP Serum 0.2 Agglutination 7 days at 2-8 C 3 Months at -20

Hemolysis, Icteric & Lipemic serum 1 day 2 hrs Qualitative

ASO Serum 0.4 Agglutination 7 days at 2-8 C 1 Months at -20 C

Hemolysis, Lipemic & icteric serum 1 day 2 hrs Qualitative

Wright agg. Test Serum 0.5 Agglutination 7 days at 2-8 C 3 Months at -20 C

Hemolysis, Lipemic & icteric serum 2 day 1 day Qualitative

2Me Serum 0.2 Agglutination 1 week at 2-8 C for longer time at -20 C

Hemolysis, Lipemic & icteric serum 2 day 1 day Qualitative

Coombs Wright Serum,Boody fluid,

CSF 1 Agglutination

2 days at 2-8 C 7 days at -20 C

Hemolysis 2 days 1 day Qualitative





Widal agg. Test Serum 1 Agglutination 7 days at 2-8 C 3 Months at -20 C

Hemolysis, Lipemic & icteric serum 1 day 2 hrs Qualitative

Indirect coombs Serum,Boody fluid 0.5 Hemagglutation 5 days at 2-8 C 1 Months at -20 C

Hemolysis 1 day 2 hrs Qualitative

VDRL (RPR) Serum 0.5 Flocculation 7 days at 2-8 C 3 Months at -20 C

Hemolysis/ avoid repeated freez/thow cycle

1 day 2 hrs Qualitative

Direct coombs Whole blood(EDTA) 0.5 Hemagglutation 1 day at RT 5 days at 2-8 C

Hemolysis/Frozen/Clotting 1 day 2 hrs Qualitative





مولکولیبخش

Test sample Method Stability interferences/Reject/Preparation befor sampling جوابدهی اورژانسی جوابدهی

CVD 1 vial whole blood PCR/Hybridization

Assay 3 days at 2-8 C or long term

at -20 C

keeping sample for more than a year or gone through more than three times freeze-thaw/ low

concentration of DNA/ the precense of PCR inhibitors

1 week 2 days

Neonatal sepsis

1 vial whole blood/CSF/Plasma

Real time-PCR 3 days at 2-8 C or long term

at -20 C



1 day 1 day

Jak 2 2 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



3 days 1 day





HTLV-1 2 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



3 days 1 day

bacterial pneomonia

Real time-PCR خون کامل/ بال / خلط 3 days at 2-8 C or long term

at -20 C



3 days 1 day

BKV/CMV/EBV 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



3 days 1 day

گاستروانتریت

باکتریاییstool Real time-PCR

3 days at 2-8 C or long term at -20 C



3 days 1 day





Real time-PCR خون کامل/ بال / خلط آسپرژیلوس3 days at 2-8 C or long term

at -20 C



3 days 1 day

BCR-ABL 5 vial whole blood Real time-PCR 1 day at 2-8 C or long term at

-80 C


concentration of RNA/ the precense of PCR inhibitors

1 week 2 days

Real time-PCR سواپ گلو/ بال پالس 21تنفسی 3 days at 2-8 C or long term

at -20 C



1 day 1 day

PCP خون کامل/ بال / خلط Real time-PCR 3 days at 2-8 C or long term

at -20 C



3 days 1 day





HCV ; ی، کیفی، کم

ژنوتایپ1 vial whole blood Real time-PCR

1 day at 2-8 C or long term at -80 C



1 week 1 day

HBV ; یکیفی، کم 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



1 week 1 day

BK/JCV 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



1 week 1 day

CMV 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



3 days 1 day





HSV CSF/ whole blood/swab


at -20 C



1 day 1 day

HLA B27 1 vial whole blood PCR 3 days at 2-8 C or long term

at -20 C



1 week 2 days

HLA B51 1 vial whole blood PCR 3 days at 2-8 C or long term

at -20 C



1 week 2 days

HLA Typing 5 vial whole blood PCR 3 days at 2-8 C or long term

at -20 C



1 week 3 days





TB / خون کامل/ بال / خلط

بافتReal time-PCR




3 days 1 day

CSF/ whole blood Real time-PCR بروسال 3 days at 2-8 C or long term

at -20 C



3 days 1 day

vial whole blood Real time-PCR 1 کاندیدا3 days at 2-8 C or long term

at -20 C



3 days 1 day

MTHFR 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C



1 week 1 day





vial whole blood Real time-PCR 1 5فاکتور 3 days at 2-8 C or long term

at -20 C



1 week 1 day

vial whole blood Real time-PCR 1 2فاکتور 3 days at 2-8 C or long term

at -20 C



1 week 1 day

HIV 1 vial whole blood Real time-PCR 1 day at 2-8 C or long term at

-80 C



3 days 1 day

آلرژی تنفسی و

غذاییSerum Hybridization Assay


keeping sample for more than a year or gone through more than three times freeze-thaw

1 week 3 days





Torch IgG Serum Hybridization Assay 3 days at 2-8 C or long term

at -20 C keeping sample for more than a year or gone through more than three times freeze-thaw

1 week 3 days

warfarin dose 1 vial whole blood Real time-PCR 3 days at 2-8 C or long term

at -20 C

Inappropriate anticoagulant like heparin-keeping sample for more than a year or gone through more than three times freeze-thaw/ low concentration

of DNA/ the precense of PCR inhibitors

3 days 1 day

STD اش در محیط برRPMI PCR 3 days at 2-8 C or long term

at -20 C



1 week 3 days

NRAS/KRAS بلوک پارافینه Sequencing RT low concentration of DNA/ the precense of PCR

inhibitors 10 days 3 days





HPV, HPV Genotaype

ادرار،زگیل،بلوک :آقایان

پارافینهPCR/Hybridization


at -20 C



3 days 2 days

زگیل، نمونه : خانمها

سرویکال، بلوک پارافینهPCR/Hybridization


at -20 C



3 days 2 days

کوانتیفرونلوله های مخصوص در بخش

مولکولیElisa


keeping sample for more than a year or gone through more than three times freeze-thaw

1 week 3 days





vial whole blood Real time-PCR 1 هموکروماتوز3 days at 2-8 C or long term

at -20 C



1 week 3 days

Bacterial meningitis

CSF/ 1 vial whole blood


at -20 C



3 days 1 days


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