Thermo Scientific Accucore HPLC Columnsapps.thermoscientific.com/media/SID/Europe Region/PDF... ·...
Transcript of Thermo Scientific Accucore HPLC Columnsapps.thermoscientific.com/media/SID/Europe Region/PDF... ·...
Thermo Scientific Accucore HPLC Columns
Stephen LukeProduct ManagerJune 2011
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Fast Chromatography - Understanding the Drivers
• Analytical Scientist want
FAST CHROMATOGRAPHY
Speed 62%
Resolution 23%
Sensitivity 7%
Other 8%
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Fast HPLC – Particle Evolution
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Core Enhanced TechnologyTM
Solid Core Particles – 2.6µm diameter particles with a solid core generate high speed, high resolution separations without excessive backpressure
Advanced Bonding Technology – Optimised phase bonding creates a series of high coverage, robust phases
Tight Control of Particle Diameter – Enhanced selection process keeps particle size distribution to a minimum and produces high efficiency columns
Automated Packing Process -Enhanced automated procedures ensure that all columns are packed with the highest quality
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Linear velocity of mobile phase (mm/s)Accucore 2.6µm 5µm 3µm <2µm
Core Enhanced Technology - Efficiency
Highest efficiency and lowest rate of efficiency loss with flow rate with Core Enhanced Technology
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Accucore RP-MS 2.6um 5um 3um <2umN (/)
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Core Enhanced Technology – Efficiency & Speed
Efficiency increasing
Highest plate generation rate with Core Enhanced Technology
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Core Enhanced Technology – Low Pressure
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Accucore RP-MS 2.6µm <2µm 3µm 5µm
Columns: 100 x 2.1 mmMobile phase: H2O / ACN (1:1)Temperature: 30 °C
600 bar limit
HPLC pressure limit
Pressures below 600 bar over optimum flow rate range with Core Enhanced Technology
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• Ultimate Core Performance to Maximize Your Investment
• Rugged and reproducible solid core particles• Fast separations with superb resolution• Low backpressures
AccucoreTM HPLC Columns
Core Enhanced Technology
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Accucore HPLC Columns – Features and Benefits
Ultimate Core Performance Maximizing Your Investment
Fast Separations Increase sample throughput and decrease solvent costs
High Peak Capacity Separate and identify more peaks
Increased Sensitivity Detect trace amounts
Lower Pressure Use existing HPLC instruments or prolong the life of UHPLC instruments
Loading Capacity Analyze a wide range of concentrations
Reproducible Chromatography Have confidence in your separations
Long Lifetime Use columns for longer
Wide Selectivity Use the right column for your analysis
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Accucore HPLC Columns - Specifications
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Accucore HPLC Columns - Characterization
Hydrophobic Interactions
Secondary Interactions
Acidic Interactions
Hydrophobic retention
Base activity Acid interaction
HR BA AI Hydrophobic selectivity
Chelation
Ion exchange capacity pH 2.7
HS C IEX (2.7) Steric selectivity Ion exchange
capacity (pH 7.6)
SS IEX (7.6) Hydrogen bonding capacity
HBC
HR K’ Pentylbenzene
HS α Butylbenzene / Pentylbenzene
SS α Triphenylene / o-Terphenyl
HBC α Caffeine / Phenol
BA tf Amitripyline
C tf Quinizarin
IEX(7.6) α Benzylamine / Phenol
AI tf 4-Chlorocinnamic acid
IEX(2.7) α Benzylamine / Phenol
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Accucore HPLC Columns – Phase Range
RP-MS Optimized for MS detection, excellent combination of speed and quality of separation
Phenyl-Hexyl Unique selectivity for aromatic and moderately polar
analytes
C18 Optimum retention for non-polar analytes
PFP Alternative
selectivity to C18, particularly for
halogenated analytes
aQ Compatible with 100% aqueous mobile phases, special selectivity for polar analytes
HILIC
Enhanced Retention of polar
and hydrophilic analytes
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Accucore RP-MS
• Optimized for MS detection• Bleed tested
• Excellent Peak Shapes• Fewer mobile phase modifiers
• Best Combination of Speed and Efficiency
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Accucore C18
• Optimum retention of non-polar compounds • Hydrophobic interaction mechanism• Separate a broad range of analytes
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Accucore aQ
• Retention and Resolution of Polar Analytes• Polar Endcapped C18 Stationary Phase for Alternative Selectivity• Ideal for Highly Aqueous Mobile Phases
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Accucore Phenyl-Hexyl
• Mixed-Mode Selectivity for Aromatic and Moderately Polar Analytes• Enhanced Pi-pi Interactions with Aromatics• Moderate Hydrophobicity
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Accucore PFP
• Alternative Selectivity to C18• Extra Retention for Halogenated Species• Unique Selectivity for Non-halogenated Polar Compounds
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Accucore HILIC
• Enhanced Retention of Polar and Hydrophilic Analytes• Alternative Selectivity to C18 without Ion-pair or Derivatization• Improved Sensitivity for MS detection
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Accucore HPLC Columns – SpeedAccucore C18 2.6µm, 50 mm x 2.1 mmMobile phase A: water; B: AcetonitrileGradient: Min %B
0 400.4 950.80 95
Flow rate : 1.0 mL /minInjection volume : 1 µLTemperature: 45 °CDetection: UV at λ = 258 nm
Pressure: 113 bar
• Analytes: 1. 2-Pentanone2. Acetophenone3. 2-Heptanone4. Butyrophenone5. Hexanophenone6. Octanophenne7. Decanophenone
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9Time (min)
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High resolution separation 6 times faster than a 150x4.6mm, 5µm column – at low pressure
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Accucore HPLC Columns – Resolution
Accucore PFP 2.6µm, 100 mm x 2.1 mmMobile phase A : 0.1% Formic acid in waterMobile phase B: 0.1% Formic acid in AcetonitrileGradient: 15-30%B in 7 minutesFlow rate: 600 µL/minInjection volume: 2 µLTemperature: 50 °CDetection: UV at 270 nm
Pressure: 270 bar
Minutes0 2 4 6 8
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1 3,4 - Dimethoxyphenol
2 2,6 - Dimethoxyphenol
3 2,6 - Difluorophenol
4 3,5 - Dimethoxyphenol
5 2,4 - Difluorophenol
6 2,3 - Difluorophenol
7 3,4 - Difluorophenol
8 3,5 - Dimethylphenol
9 2,6 - Dimethylphenol
10 2,6 - Dichlorophenol
11 4 – Chloro-3-Methylphenol
12 4-Chloro-2-Methylphenol
13 3,4 - Dichlorophenol
14 3,5 - Dichlorophenol
Baseline resolution of 14 positional isomers in 7-minutes
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Accucore HPLC Columns – Ruggedness
Efficiency (o-Xylene)
Asymmetry (o-Xylene)
Accucore RP-MS 2.6µm 100x 2.1mm IDMobile Phase: 60/40 ACN/H2OFlow Rate: 400 µL/minInjection Volume: 1 µLColumn Temp: 30°C
Accucore RP-MS 2.6µm 100x 2.1mm IDMobile Phase A: Water (0.05% TFA)Mobile Phase B: Acetonitrile (0.05% TFA)Wash: H2O (0.05% TFA)Injection Volume: 1 µLColumn Temperature: 30°C
4,000 + isocratic test injections with no decrease in performance
6,000 + fast gradient injections with no change in retention
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• Ultimate Core Performance to Maximize Your Investment
• Rugged and reproducible solid core particles• Fast separations with superb resolution• Low backpressures
Conclusion
Core Enhanced Technology