Susu Pasteurisasi_Kevin Cahyadi_13.70.0096_D3_UNIKA_SOEGIJAPRANATA

8/15/2019 Susu Pasteurisasi_Kevin Cahyadi_13.70.0096_D3_UNIKA_SOEGIJAPRANATA

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Acara I

SUSU PASTEURISASI

LAPORAN RESMI PRAKTIKUM

TEKNOLOGI PENGOLAHAN SUSU

Disusun oleh:

Nama: Kevin ah!a"i

NIM: #$%&'%''()Kelom*o+: D$

PROGRAM STUDI TEKNOLOGI PANGAN

,AKULTAS TEKNOLOGI PERTANIANUNI-ERSITAS KATOLIK SOEGI.APRANATA

SEMARANG

/'#)

1. TOPIK DAN TUJUAN PRAKTIKUM

1.1. Topik


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/

Pa"a 0an11al $' Mei /'#) +lo0er D *ra+0i+um 0e+onolo1i *en1olahan susu mela+u+an

*erco2aan susu *as0eurisasi% Perco2aan "ila+u+an "i La2ora0orium Re+a!asa Pan1an

*a"a *u+ul #3%'' 4I5 hin11a #&%'' 4I5% Perco2aan "ila+u+an "en1an mela+u+an /

6enis *emanasan *a"a susu !an1 *er0ama *a"a suhu &/o selama #3 "e0i+ "an !an1

+e"ua "ila+u+an *emanasan *a"a suhu )'o selama $ meni0%

1.2. Tujuan Praktikum

Tu6uan "ari *ra+0i+um susu *as0eurisasi +ali ini a"alah men1e0ahui e7e+0ivi0as susu

"en1an *emanasan *a"a me0o"e *as0eurisasi "alam +ai0ann!a men1on0rol 6umlah

2a+0eri%

2. HASIL PENAMATAN

!.

!.1. Ta"#$ P#n%amatan

&. Hasil *en1ama0an susu *as0eurisasi "a*a0 "iliha0 *a"a Ta2el #%

'.

(. Ta2el #% Susu Pas0eurisasi

).

Kel

*. Perla+uan +. .umlah 0o0al 2a+0eri

8,U9ml

1,.

D#

11. Susu se2elum *as0eurisasi 12. 3;)

2(. Susu se0elah *as0eurisasi suhu )/° selama $

meni0

2). ?$;'


3/13

$

D> "e0i+

&&. Susu se0elah *as0eurisasi suhu )/° selama $

meni0

&'. $;#;3


4/13

>

((.

PEM/AHASAN

().

(*. Pa"a *erco2aan +ali ini "ila+u+an u6i e7e+0ivi0as suhu *emanasan *as0eurisasi

"alam men1on0rol 6umlah mi+ro2a *a"a susu% Menuru0 Ir7an B Riana 8/'#$ *a"a

6urnal mere+a men!a0a+an 2aha *roses *as0eurisasi meru*a+an *emanasan *a"a

sua0u *ro"u+ "en1an suhu "i2aah 0i0i+ "i"ihn!a "en1an 0u6uan mem*erlam2a0

a0au mem2unuh 2a+0eri *a0o1en !an1 a"a "i"alam *ro"u+% Den1an "ila+u+ann!a

*as0eurisasi *ro"u+ susu "a*a0 menin1+a0 umur sim*ann!a% Proses *as0eurisasi

"a*a0 "ila+u+an "en1a LTLT 8 Low Temperature, Long Time a0au "en1an cara

HTST 8 High Temperature Short Time% Susu hasil *roses *as0eurisasi memili+i

"a!a 0ahan "i2aah susu *roses UHT +arena *emanasann!a "i2aah 0i0i+ "i"ih

sehin11a 2a+0eri 0ermo7ili+ masih 0er"a*a0 *a"a susu% Susu *as0eurisasi "a*a0

2er0ahan selama = hari *a"a suhu re7ri1era0or 84annia0ie "an Hanum; /'#3%

A2u2a+ar 8/''# menam2ah+an selama *roses *as0eurisasi la*isan +rim *a"a susu


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3

"a*a0 2er+uran1 % Mai0imu et al 8/'#$ men!a0a+an 2aha *enam2ahan "aun

aileru "a*a0 menin1+a0+an "a!a sim*an susu *as0eurisasi

(+.

),. Susu se1ar sen"iri meru*a+an 2ahan *an1an !an1 mu"ah rusa+ +arena 0in11inan!a

*ro*orsi "an +an"un1an 1iCi sehin11a *erlu "ila+u+an *roses *as0eurisasi% Proses

*encemaran susu sen"iri "a*a0 0er6a"i "ari *roses *emerahan susu !an1 0i"a+ s0eril;

+on0aminan u"ara; *erala0an a0au +an"an1 0erna+ !an1 +o0or; "ari *a+an 0erna+;

a0au in7e+si *a"a +elen6ar 0erna+ 8ah!ono et al ; /'#$% Lu et al 8/'#$

menam2ah+an 2aha +on0aminasi susu se1ar 6u1a "a*a0 0er6a"i *a"a *roses

"is0ri2usi susu "iamana un0u+ 0e0a* men6a1a +uali0as susu a1ar 0e0a* se1ar suhu

selama *roses "is0ri2usi harus "i6a1a a1ar 0i"a+ mele2ihi >'o +arena "a*a0

menin1+a0+an 6umlah 2a+0eri meso7ili+ "i"alamn!a%

)1.

)2. 5a+0eri !an1 2iasa 0um2uh *a"a susu se1ar "ian0aran!a 2a+0eri asam la+0a0; 2a+0eri

+oli7orm; 2a+0eri asam 2u0ira0; 2a+0eri asam *ro*iona0; 2a+0eri *a0o1en; ser0a

2a+0eri *em2usu+8Gaman B Sherrin10on; #((>% Sui0o 8/'#' "alam 6urnaln!a

men!a0a+an 2aha 6enis 2a+0eri *a0o1en "an *em2usu+ !an1 *alin1 "ihin"ari%

.enis 2a+0eri *a0o1en con0ohn!a a"alah E. coli !an1 "a*a0 men!e2a2+an "iare *a"a

manusia +arena men1hasil+an en0ero0o+sin% 5a+0eri Salmonella s*% "a*a0

men!e2a2+an in7e+si *a"a usus ser0a meran1san1 *em2en0u+an sel sel ra"an1

*a"a 2a1ian *enceranaan% Un0u+ 2a+0eri S % aureus "a*a0 men!e2a2+an "iare; mual;

"an mun0ah% Semen0ara 6enis 2a+0eri *em2usu+ con0ohn!a a"alah Micrococcus

s*% , Pseudomonas s*%; "an Bacillus s*% !an1 "a*a0 men!e2a2+an susu men6a"i

2erlen"ir ser0a asam +arena 2a+0eri 0erse2u0 "a*a0 men1urai *ro0ein "an merom2a+

lema+%)!.

)&. Pa"a *erco2aan ini "iaali "en1an men!ia*+an / 2uah 2o0ol +aca !an1 su"ah s0eril

"an +emu"ian masin1 masin1 2o0ol "iisi susu se1ar se2an!a+ /'' ml% Masin1

masin1 2o0ol 0erse2u0 +emu"ian "i2eri *erla+uan *emanasan !an1 2er2e"a% 5o0ol A

"i*anas+an *a"a suhu &/o selama #3 "e0i+ "an 2o0ol 5 "i*anas+an *a"a suhu

)/o selama $ meni0% Se0elah i0u susu "iam2il se2a1ian un0u+ u6i analisa mi+ro2a

a+hirn!a "an sisan!a "isim*an "i "alam re7ri1era0or% Proses analisa "iaali "en1an


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)

*en1enceran "en1an aua"es% Proses *en1enceran harus "e+a0 a*i 2unsen "an

"aerah se+i0ar su"ah "isem*ro0 al+ohol a1ar 0i"a+ men1+on0aminasi 6alann!a

analisa% Proses *en1enceran sen"iri "ila+u+an a1ar laru0an 0i"a+ 0erlalu *e+a0

sehin11a hasil analisa 0i"a+ 0erlalu 2an!a+% 8Ha"ioe0omo; #(($% Proses

*en1enceran "ila+u+an "en1an men1am2il sam*el susu se2an!a+ / ml "an

"iencer+an "en1an aua"es *a"a 0a2un1 rea+si% Un0u+ sam*el susu !an1 2elum

"i*as0eurisasi "ila+u+an *en1enceran #'F3 "an #'F)% Un0u+ sam*el susu !an1 su"ah

"i *as0eurisasi "ila+u+an *en1enceran #'#; #'F/; "an #'F$% Se0elah "iencer+an *roses

selan6u0n!a a"alah *enuan1an +e caan *e0ri !an1 su"ah 0er"a*a0 me"ia a1ar NA

"i"alam caan%

)'.

)(. Me0o"e *enuan1an !an1 "ila+u+an a"alah pour plate "imana *enuan1an "ila+u+an

se0elah me"ia 0er2en0u+% aan +emu"ian "iin+u2asi selama # hari "an hasiln!a

"ihi0un1 men11una+an colony hand counter % Hasil *erhi0un1an "ian11a* vali" 2ila

6umlah +oloni !an1 0um2uh 2era"a *a"a ren0an $' $'' +oloni 8Ha"ioe0omo;

#(($% Me"ia !an1 "i1una+an un0u+ analisa mi+ro2a +ali ini a"alah me"ia a1ar

NA% Me"ia a1ar NA menuru0 Ha!es 8#((# meru*a+an me"ia !an1 2iasa

"i1una+an un0u+ +ul0ivasi mi+roor1ansime% Me"ia NA men1an"un1 nu0risi se*er0i

; H; O; N; S; "an P sehin11a "a*a0 men"u+un1 *er0um2uhan "ari mi+roor1anisme

0erse2u0% .enis mi+roor1anisme !an1 2iasa "i0um2uh+an *a"a me"ia NA a"alah

+hamir "an 2a+0eri 6enis he0ero0o7 !an1 mem2u0uh+an +an"un1an lema+ "an

*ro0ein !an1 0in11i un0u+ "a*a0 0um2uh% Selain men1an"un1 ; H; O; N; S; "an P

me"ia a1ar NA 6u1a men1an"un1 ,e; M1; K; "an a "alam 6umlah +ecil +arena

0er2ua0 "ari *oli*e*0on% Me"ia NA sen"iri 0er2ua0 "ari beef etract "en1an *H $;

"an a1ar "en1an *H #3; *oli*e*0on "en1an *H 3 "imana hasil a+hir *H secara+eseluruhan a"alah );=8PelcCar B Rei"% Sam*el !an1 su"ah "iin+u2asi selama #

hari 0erse2u0 +emu"ian "ihi0un1 "en1a colony counter "an 6umlah ,U9ml "ihi0un1

"en1an rumus "an "i2an"in+an hasiln!a "en1an s0an"ar nasional%

)).

)*. 5er"asar+an SNI '#F$(3#F#((3 6umlah ma+simal +an"un1an mi+ro2a *a"a susu

se1ar a0au se2elum "i *as0eurisasi a"alah #


7/13

&

a"alah $

,U9ml "an *a"a+elom*o+ D> men1hasil+an 6umlah 0o0al 2a+0eri );= ,U9ml% Hasil 0erse2u0 0i"a+ 6i+a

"i2an"in1+an "en1an 0eori A2u2a+ar 8/''# !an1 men!a0a+an *roses HTST *a"a

suhu &/o selama #3 "e0i+ le2ih 2ai+ "alam men1uran1i 6umlah mi+ro2a *a"a susu

"i2an"in1+an *roses LTLT *a"a suhu )/o selama $ meni0% Te0a*i masih menuru0

A2u2a+ar 8/''# se2enarn!a an0ar *roses HTST "an *roses LTLT 0i"a+ memili+i

*er2e"aan !an1 si1ni7i+an% Da*a0 "isim*ula+an 2aha hasil *erco2aan


8/13

=

menun6u+an 2aha a+0u *emanasan le2ih mem*en1aruhi 6umlah mi+ro2a *a"a

susu "i2an"in1+an suhu *emanasan% Sema+in lama a+0u *as0eurisasi a+an

mem2ua0 +an"un1an mi+ro2a *a"a susu sema+in 2er+uran1

*1.

*2. KESIMPULAN

*!.

• Pas0eurisasi meru*a+an *roses *emanasan !an1 "ila+u+an *a"a susu "en1an suhu

"i2aah 0i0i+ "i"ih%

• Pas0eurisasi 2er0u6uan un0u+ me2unuh "an men1ham2a0 2a+0eri +on0aminan *a"a

susu%

• Umur sim*an susu *as0eurisasi 0erhi0un1 *en"e+ "imana *a"a suhu re7ri1era0or

susu *as0eurisasi "a*a0 2er0ahan selama = hari%

• Proses *as0eurisasi "a*a0 "ila+u+an secara HTST 8 High Temperature Short Time

a0au LTLT 8 Low Temperature Long Time%

• 5er"asar+an SNI '#F$(3#F#((3 6umlah ma+simal +an"un1an mi+ro2a *a"a susu

se1ar a0au se2elum "i *as0eurisasi a"alah # ,U9ml%

• Susu se1ar "a*a0 men1an"un1 6umlah mi+ro2a 2erle2ih +arena *roses *enan1anan

*ro"u+si !an1 0i"a+ ase*0is "an 6u1a *roses "is0ri2usi !an1 0i"a+ 2enar%• Hasil a+hir "ari *roses *emanasan HTST "an TLTL 0i"a+ 0erlalu si1ni7i+an%

• Hasil *erco2aan menun6u+an 2aha lama *emanasan le2ih 2er*en1aruh "alam

men1uran1i 6umlah mi+ro2a "i2an"in1 suhu *emanasan%

*&.

*'.

*(.

*). Semaran1; = .uni /'#)

**. Pra+0i+an; Asis0en Dosen:*+. FGra!00a In0annia

+,. FRr% Panulu

P% M%

+1. Kevin ah!a"i

+2. #$%&'%''()

+!. DA-TAR PUSTAKA

+&.


9/13

(

+'. A2u2a+ar; Tri!an0ini; R% Sunarlim; H% Se0i!an0o; an" Nur6annah% 8/''#% Effect !f

Temperature "nd Time !f Pasteuri#ation !n The Mil$ %uality &uring Storage%

.urnal Ilmu Terna+ "an -e0eriner )8#:>3F3'%

+(.

+). ah!ono; D%; Pa"a1a; M%%%; B Sai0ri; M% E% 8/'#$% Ka6ian Kuali0asMi+ro2iolo1is 8Total Plate 'ount (TP'); Enterobacteriaceae "an Staphylococcus

"ureus Susu Sa*i Se1ar "i Kecama0an Krucil Ka2u*a0en Pro2olin11o% .urnal

Ilmu "an Te+nolo1i Hasil Terna+% Hal #F= -ol% =; No% #% Universi0as 5rai6a!a;

Malan1%

+*.

++. ,an1Fhen 4u% henF4ei han1; In1FLu Shih% /'#$% O*0imiCa0ion o7 The

Pro"uc0ion an" harac0eriCa0ion o7 Mil+ lo00in1 EnC!mes 2! Bacillus subtillis

Na00o% -ol / No $$%

1,,. Gaman; P%M B K%5% Sherrin10on% 8#((>% Ilmu Pan1an; Pen1an0ar Ilmu Pan1an;

Nu0risi "an Mi+ro2iolo1i% Universi0as Ga"6ah Ma"a *ress% o1!a+ar0a%

1,1. Ha"ioe0omo; R%S% 8#(($% Mi+ro2iolo1i Dasar "alam Pra+0e+ : Te+ni+ "an

Prose"ur Dasar La2ora0orium% PT Grame"ia% .a+ar0a%

1,2.

1,!. Ha!es; 4a!lan" .% Las; E"ar" R% Handboo$ of Pesticide Toicology *olume +

-eneral Principles% Ne or+: Aca"emic Press; Inc% #((#%

1,&. Kurniaan; I% B Pu0ri; R%D%M% 8/'#$% Ala0 Peman0au Kes0a2ilan Pas0eurisasi

Susu% .urnal Te+ni+ Ele+0ro -ol% 3 No% /% Universi0as Ne1eri Semaran1%

1,'.

1,(. Mai0imu; %-%; Le1oo; A%M%; B AlF5aarri; A%N% 8/'#$% Kara+0eris0i+

Mi+ro2iolo1is; Kimia; ,isi+ "an Or1anole*0i+ Susu Pas0eurisasi "en1an

Penam2ahan E+s0ra+ Daun Aileru 8rightia 'alycina Selama Pen!im*anan%

.urnal A*li+asi Te+nolo1i Pan1an% -ol% / No%#% Universi0as Di*one1oro;

Semaran1%

1,).

1,*. S0an"ar Nasional In"onesia% 8#((3% No%'#F$(3#F#((3 S0an"ar Mu0u Pro"u+ Susu

"an olahann!a 5er"asar+an S0an"ar Nasional In"onesia 8SNI 5u+u I% Dire+0ora0

Pen1olahan "an Pemasaran Hasil Pe0erna+an% Dir6en 5ina Pen1olahan "an

Pemasaran Hasil Per0anian% De*ar0emen Per0anian%1,+.

110. Sui0o; 4% 8/'#'% 5a+0eri !an1 Serin1 Mencemari Susu: De0e+si; Pa0o1enesis;

E*i"emiolo1i; "an ara Pen1en"aliann!a% /urnal Litbang Pertanian, 01(2). Balai

Peng$a3ian Te$nologi Pertanian 4ogya$arta.

111.

112. 4annia0ie; -% B Hanum; % 8/'#3% Kuali0as Susu Pas0eurisasi Komersil% A1ri*e0

-ol #3; No% /% Universi0as Lam*un1

11!. LAMPIRAN

11&.

11&.1. P#r0itun%an

11'. Rumu


10/13

#'

11(. CFU

ml =

1

Faktor pengenceran x jumlahkoloni

11).

11*. Se2elum Pas0eurisasi

F Pen1enceran #'F3

11+.CFU

ml =

1

10−5

x56

12,. @ 3)2

12(.Ma+a men11una+an *en1enceran +ecil; !ai0u 5,6 x106

12).

12*.Se0elah Pen1enceran

12+.Kelom*o+ D#

1!,.5o0ol A :

1!1.Pen1enceran #'F#

1!2.CFU

ml

=1

10

−1 x21

1!!. @ /#'

1!&.Pen1enceran #'F/

1!'.CFU

ml =

1

10−2 x 84

1!(. @ =;>2

21,. Ma+ men11una+an *en1enceran +ecil;

!ai0u 3 x103 ,U9ml

211.

212.


11/13

##

1&&.5o0ol A :

1&'.Pen1enceran #'F#

1&(.CFU

ml =

1

10−

1

x24

1&). @ /;>

1(*. 3,6 x 10

4

5,5 x 103=65>2

1(+.Ma+a men11una+an *en1enceran

21!. 5o0ol 5 :

21&. Pen1enceran #'F#

21'.CFU

ml =

1

10−

1

x6

21(. @ )'

21). Pen1enceran #'F/

21*.CFU

ml =

1

10−2

x0

21+. @ '

22,. Pen1enceran #'F$

221.CFU

ml =

1

10−3 x1

222. @ #


12/13

#/

+ecil; !ai0u 5,5 x 103 ,U9ml

1),.

1)1.Kelom*o+ D>

1)2.5o0ol A :

1)!.Pen1enceran #'F#

1)&.CFU

ml =

1

10−1

x14

1)'. @ #;>;3

2'!. ?$;'


13/13

#$

1+'. @ $;/

Susu Pasteurisasi_Kevin Cahyadi_13.70.0096_D3_UNIKA_SOEGIJAPRANATA

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