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KROMATOGRAFI GAS (GAS CHROMATOGRAPHY)

A.  PendahuluanKromatografi gas-cair (GLC), atau romatografi gas (GC), m!ru"aa# $!#is

romatografi %a#g &igu#aa# &a'am imia orga#i u#tu "!misaa# &a# a#a'isis GC &a"at&igu#aa# u#tu m!#gu$i !mur#ia# &ari *aa# t!rt!#tu, atau m!misaa# *!r*agaiom"o#!# &ari cam"ura# +a'am *!*!ra"a situasi, GC &a"at m!m*a#tu &a'amm!#gi&!#tifiasi s!*ua om"'!s

B.  Landasan TeoriKromatografi Gas a&a'a "ros!s "!misaa# cam"ura# m!#$a&i om"o#!#-

om"o#!##%a &!#ga# m!#ggu#aa# gas s!*agai fas! *!rg!ra %a#g m!'!ati suatu'a"isa# s!ra"a# (sor*!#) %a#g &iam S!'uru *!#tu romatografi t!r&iri &ari fas! &iam &a#fas! g!ra S!*agaima# &a'am &a'am fas! gas-cair, Kromatografi gas fas! g!ra &a# fas!&iam#%a &ia#tara#%a

• Fas! g!ra a&a'a gas &a# .at t!r'arut t!r"isa s!*agai ua" P!misaa# t!rca"ai&!#ga# "artisi sam"!' a#tara fas! gas *!rg!ra

• Fas! &iam *!ru"a caira# &!#ga# titi &i&i ti#ggi (ti&a mu&a m!#gua") %a#g

t!riat "a&a .at "a&at "!#u#$a#g#%a

/agaima#a !c!"ata# suatu s!#%aa t!rt!#tu *!rg!ra m!'a'ui m!si#, aa#*!rga#tu#g "a&a s!*!ra"a 'ama atu %a#g &ia*isa# u#tu *!rg!ra &!#ga# gas &a#s!*a'i#%a m!'!at &!#ga# caira# &!#ga# $a'a# %a#g sama

+a'am romatografi gas, fas! %a#g *!rg!ra (atau 0mo*i'! "as!0) a&a'a s!*uao"!rator gas, %a#g *iasa#%a gas mur#i s!"!rti !'ium atau %a#g ti&a r!acti1! s!"!rti gas#itrog!# Statio#ar% atau fasa &iam m!ru"aa# taa" miroso"is 'a"isa# cair atau "o'im!r 

%a#g m!#&uu#g gas mur#i, &i &a'am *agia# &ari sist!m "i"a-"i"a aca atau 'ogam %a#g&is!*ut o'om I#strum!# %a#g &igu#aa# u#tu m!'aua# romatografi gas &is!*ut gascromatogra" (atau 0a!rogra"0, 0gas "!misa0)

s!#%aa gas %a#g s!&a#g &ia#a'isis *!ri#t!rasi &!#ga# &i#&i#g o'om %a#g &i'a"isi&!#ga# *!r*agai taa"a# statio#ar% I#i m!#%!*a*a# s!tia" om"'!s ! !'ut! &i atu%a#g *!r*!&a, %a#g &i!#a' s!*agai i#gata# atu %a#g om"'!s P!r*a#&i#ga# &arii#gata# a'i %a#g m!m*!ria# !gu#aa# a#a'isis GC-#%a

Kromatografi gas %a#g "a&a "ri#si"#%a sama &!#ga# romatografi o'om (s!rta%a#g 'ai##%a *!#tu romatografi, s!"!rti HPLC, TLC), ta"i m!mi'ii *!*!ra"a "!r*!&aa#"!#ti#g P!rtama, "ros!s m!misaa# com"ou#&s &a'am cam"ura# &i'aua# a#tarastatio#ar% fas! cair &a# gas fas! *!rg!ra, s!&a#ga# "a&a romatografi o'om %a#g

s!im*a#g a&a'a taa" %a#g so'i& &a# *!rg!ra a&a'a fas! cair (2a&i, #ama '!#ga""ros!&ur a&a'a 0romatografi gas-cair0, m!ru$u ! "o#s!' &a# statio#ar% taa"a#,masi#g-masi#g) K!&ua, m!'a'ui o'om %a#g 'o'os taa" gas t!r'!ta &i s!*ua o1!#&ima#a t!m"!ratur gas %a#g &a"at &io#tro', s!&a#ga# romatografi o'om (*iasa#%a)ti&a m!mi'ii o#tro' s!"!rti suu K!tiga, o#s!#trasi %a#g ma$!mu &a'am fas! gasa&a'a a#%a sa'a satu fu#gsi &ari t!a#a# ua" &ari gas

Kromatografi gas $uga miri" &!#ga# "!caa# "!#%u'i#ga#, ar!#a !&ua "ros!sm!misaa# om"o#!# &ari cam"ura# t!rutama *!r&asara# titi &i&i (atau t!a#a# ua")"!r*!&aa# 3amu#, "!caa# "!#%u'i#ga# *iasa#%a &igu#aa# u#tu m!misaa#

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om"o#!# cam"ura# "a&a sa'a *!sar, s!&a#ga# GC &a"at &igu#aa# "a&a sa'a %a#g'!*i !ci' (%a#i microsca'!)

Kromatografi gas t!ra&a#g $uga &i!#a' s!*agai ua"-taa" romatografi (4PC),atau gas-cair romatografi "artisi (GLPC) A't!r#atif #ama i#i, s!rta masi#g-masi#gsi#gata#, s!ri#g &it!mua# &a'am 'it!ratur i'mia

+iagram a'ir romatografi gas-cair  

C.  Mekanisme kerja GC dan Komponen dalam Kromatografi Gas :1.  ase Mo!il "Gas Pem!a#a$.

Fasa mo*i' (gas "!m*aa) &i"aso &ari ta#i m!'a'ui "!#gatura# "!#gura#ga#t!a#a# K!mu&ia# m!m*aa cu"'ia# 'a#gsu#g ! &a'am o'om 2ia a' i#i t!r$a&i,cu"'ia# ti&a m!#%!*ar s!*!'um "ros!s "!misaa# Cara i#i coco u#tu cu"'ia# %a#gmu&a m!#%!ra"

Gas "!m*aa i#i arus *!rsifat i#!rt &a# arus sa#gat mur#i S!ri#ga'i gas"!m*aa i#i arus &isari#g u#tu m!#aa# &!*u ua" air &a# osig!# Gas s!ri#g

&igu#aa# a&a'a 35, H5 H! &a# Ar%.

 

&njeksi sampelS!$um'a !ci' sam"!' %a#g aa# &ia#a'isis &ii#$!sia# "a&a m!si# m!#ggu#aa#

s!m"rit !ci' 2arum s!m"rit m!#!m*us '!m"!#ga# ar!t t!*a' (L!m"!#ga# ar!t i#i&is!*ut s!"tum) %a#g ma#a aa# m!#gu*a *!#tu#%a !m*a'i s!cara otomatis !tias!m"rit &itari !'uar &ari '!m"!#ga# ar!t t!rs!*ut

I#$!tor *!ra&a &a'am o1!# %a#g ma#a t!m"!ratur#%a &a"at &io#tro' O1!#t!rs!*ut cuu" "a#as s!i#gga sam"!' &a"at m!#&i&i &a# &ia#gut ! o'om o'! gas"!m*aa misa'#%a !'ium atau gas 'ai##%a

Fas! *!rg!ra &a'am romatografi i#i a&a'a gas, %a#g "a'i#g 'a.im a&a'a !'ium,i&rog!#, atau #itrog!# Kom"!#!# "i'ia# gas s"!m*aa t!rutama t!rga#tu#g "a&aarat!risti &!t!tor Kromatografi gas om!rsia' *iasa#%a m!#%!&iaa# atu" "!#gatur 

tam*aa# u#tu "!#g!#&a'ia# t!a#a# %a#g *ai "a&a i#'!t o'om +!at i#'!t o'om a&asuatu a'at &ima#a sam"!'-sam"!' &a"at &imasuua# !&a'am a'ira# gas "!m*aaSam"!'-sam"!' t!rs!*ut *isa *!ru"a gas atau caira# %a#g mu&a m!#gua" Lu*a#g i#$!si&i"a#asa# agar sam"!' cair t!rua"a# &!#ga# c!"at

'.  Kolom A'ira# gas s!'a#$ut#%a m!#!mui o'om %a#g &i'!taa# &a'am o1!# *!rt!m"!ratur 

o#sta# I#i a&a'a $a#tu#g i#strum!# t!s!*ut, t!m"at &ima#a "ros!s romartgrafi &asar 

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*!r'a#gsu#g Ko'om m!mi'i 1ariasi &a'am a' uura# &a# *aa# isia# Ta*u#g &iisi &!#ga#*aa# "a&at a'us &!#ga# 'uas "!rmuaa# *!sar %a#g r!'atif i#!rt Pa&ata# iitus!*!#ar#%a a#%a s!*ua "!#%a#gga m!a#i u#tu caira#, s!*!'um &iisi !&a'am o'om,"a&ata# t!rs!*ut &iim"r!g#asi &!#ga# caira# %a#g &ii#gi#a# %a#g *!r"ar!ra# s!*agaifasa stasio#!r s!su#ggu#%a Caira# i#i arus sta*i' &a# #o#fo'ati' "a&a t!m"!ratur o'om, "!misaa# &a# arus s!suai u#tu "!misaa# t!rt!#tu

 A&a &ua ti"! utama o'om &a'am romatografi gas-cair Ti"! "!rtama, Ko'om Partisi,*!risi *aa# "a&at i#!rt m!#%a#gga 'a"isa# ti"is caira#, &is!*ut Cromatogra"% Gas Cair (GLC)6 Ti"! !&ua, Ko'om A&sor*si, *!risi "arti!' "!#%!ra" %a#g umum#%a &igu#aa#u#tu a#a'isa gas "!rma#!# &a# %&roar*o# r!#&a, *iasa &is!*ut Cromatogra"% GasPa&at (GSC)

Ko'om *iasa#%a &i*uat &ari *a$a ta *!rarat &!#ga# "a#$a#g a#tara 7 sam"ai 8m!t!r, &!#ga# &iam!t!r i#t!r#a' sam"ai 8 mm Ko'om &igu'u#g s!i#gga &a"at &is!suaa#&!#ga# o1!# %a#g t!ro#tro' s!cara t!rmostatisKo'om &i"a&ata# &!#ga# ta#a &iatoma!,%a#g m!ru"aa# *atu %a#g sa#gat *!r"ori Ta#a i#i &i'a"isis &!#ga# caira# *!rtiti &i&iti#ggi, *iasa#%a "o'im!r 'i'i#

•  Temperatur kolom

T!m"!ratur o'om &a"at *!r1ariasi a#tara 9: oC sam"ai 59: oC T!m"!ratur o'om'!*i r!#&a &ari"a&a g!r*a#g i#$!si "a&a o1!#, s!i#gga *!*!ra"a om"o#!# cam"ura#&a"at *!ro#&!#sasi "a&a aa' o'om Ko'om m!mu'ai "a&a t!m"!ratur r!#&a &a#!mu&ia# t!rus m!#!rus m!#$a&i '!*i "a#as &i*aa "!#gaasa# om"ut!r saat a#a'isis*!r'a#gsu#g

• 

Proses pemisahan pada kolom

 A&a tiga a' %a#g &a"at *!r'a#gsu#g "a&a mo'!u' t!rt!#tu &a'am cam"ura# %a#g&ii#$!sia# "a&a o'om

• 

Mo'!u' &a"at *!ro#&!#sasi "a&a fas! &iam

•  Mo'!u' &a"at 'arut &a'am caira# "a&a "!rmuaa# fas! &iam

•  Mo'!u' &a"at t!ta" "a&a fas! gas

+ari !tiga !mu#gi#a# itu, ta satu"u# %a#g *!rsifat "!rma#!#S!#%aa %a#gm!m"u#%ai titi &i&i %a#g '!*i ti#ggi &ari t!m"!ratur o'om s!cara $!'as c!#&!ru#g aa#*!ro#&!#sasi "a&a *agia# aa' o'om 3amu#, *!*!ra"a *agia# &ari s!#%aa t!rs!*utaa# m!#gua" !m*a'i &!#ga# &!#ga# $a'a# %a#g sama s!"!rti air %a#g m!#gua" saatu&ara "a#as, m!si"u# t!m"!ratur &i*aa 7:::C P!'ua#g#%a aa# *!ro#&!#sasi '!*is!&iit s!'ama *!ra&a &i&a'am o'om

Sama a'#%a u#tu *!*!ra"a mo'!u' &a"at 'arut &a'am fas! &iam cair /!*!ra"as!#%aa aa# '!*i mu&a 'arut &a'am caira# &i*a#&i#g %a#g 'ai##%a S!#%aa %a#g '!*imu&a 'arut aa# m!#ga*isa# atu#%a u#tu &is!ra" "a&a fas! &iam s!&a#ga#s!#%aa %a#g sua 'arut aa# m!#ga*isa# atu#%a '!*i *a#%a &a'am fas! gas

Pros!s &ima#a .at m!m*agi &iri#%a m!#$a&i &ua "!'arut %a#g ti&a *!rcam"ura#ar!#a "!r*!&aa# !'aruta#, &ima#a !'aruta# &a'am satu "!'arut satu '!*i mu&a&i*a#&i#g &!#ga# "!'arut 'ai##%a &is!*ut s!*agai "artisi S!ara#g, a#&a *isa *!ra'asa#u#tu m!m"!r&!*ata# *aa gas s!"!rti !'ium ti&a &a"at &i$!'asa# s!*agai "!'arutT!ta"i, isti'a "artisi masi &a"at &igu#aa# &a'am romatografi gas-cair

Su*sta#si a#tara fas! &iam cair &a# gas /!*!ra"a mo'!u' &a'am su*sta#sim!#ga*isa# atu u#tu 'arut &a'am caira# &a# *!*!ra"a 'ai##%a m!#ga*isa# atuu#tu *!rg!ra *!rsama-sama &!#ga# gas

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(.  )etektor S!t!'a mu#cu' &ari o'om itu, a'ira# gas '!at m!'a'ui sis 'ai# &!t!tor Maa !'usi

.at t!r'arut &ari o'om itu m!#gatur !ti&as!im*a#ga# a#tara&ua sisi &!t!tor %a#g&ir!am s!cara !'!tri La$u a'ira# gas "!m*aa a&a'a a' %a#g sa#gat "!#ti#g, &a#*iasa#%a "!#guur a'ira# u#tu itu t!rs!&ia S!cara #orma' gas-gas %a#g mu#cu' &ia'ira#!'uar "a&a t!a#a# atmosfir Kar!#a "!!r$a 'a*oratorium s!cara t!rus m!#!rus t!r"a"ar o'! ua" s!#%aa-s!#%aa %a#g t!rromatogafi %a#g mu#gi# ta *ai a'au"u# a&ar#%a*iasa#%a !ci' maa 1!#ti'asi "a&a !'uara# i#strum!# arus &i"!ratia# K!t!#tua# *isa&i*uat u#tu m!#$!*a .at t!r'arut %a#g &i"isaa# s!t!'a mu#cu' &ari o'om $ia a' i#i&i*utua# u#tu "!#%!'i&ia# '!*i 'a#$ut

 A&a *!*!ra"a ti"! &!t!tor %a#g *iasa &igu#aa# +!t!tor io#isasi #%a'a &i$!'asa#"a&a *agia# *aa "!#$!'asa# i#i, m!ru"aa# &!t!tor %a#g umum &a# '!*i mu&a u#tu&i$!'asa# &ari"a&a &!t!tor a't!r#atif 'ai##%a

+!t!tor io#isasi #%a'a

+a'am m!a#ism! r!asi, "!m*aara# s!#%aa orga#i m!ru"aa# a' %a#gsa#gat om"'!s S!'ama "ros!s, s!$um'a io#-io# &a# !'!tro#-!'!tro# &iasi'a# &a'am#%a'a K!a&ira# io# &a# !'!tro# &a"at &i&!t!si

S!'uru &!t!tor &itutu" &a'am o1!# %a#g '!*i "a#as &i*a#&i#g &!#ga# t!m"!ratur o'om Ha' itu m!#g!#tia# o#&!#sasi &a'am &!t!tor2ia ti&a t!r&a"at s!#%aa orga#i &ata#g &ari o'om, a#&a a#%a m!mi'ii #%a'a i&rog!#

%a#g t!r*aar &a'am air S!ara#g, a#gga"'a *aa satu s!#%aa &a'am cam"ura# a#&aa#a'isa mu'ai masu ! &a'am &!t!tor

K!tia &i*aar, itu aa# m!#gasi'a# s!$um'a io#-io# &a# !'!tro#-!'!tro# &a'am#%a'a Io# "ositif aa# *!ratrasi "a&a ato&a si'i#&!r Io#-io# #!gatif &a# !'!tro#-!'!tro#aa# *!ratrasi "a#cara##%a masi#g-masi#g %a#g ma#a m!ru"aa# a#o&aHa' i#i s!ru"a&!#ga# a"a %a#g t!r$a&i s!'ama !'!tro'isis #orma'

Pa&a ato&a, io# "ositif aa# m!#&ata#gi !'!tro#-!'!tro# &ari ato&a &a# m!#$a&i#!tra' Pa&a a#o&a, *!*!ra"a !'!tro# &a'am #%a'a aa# &i"i#&aa# "a&a !'!tro&a

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"ositif6 io#-io# #!gatif aa# m!m*!ria# !'!tro#-!'!tro##%a "a&a !'!tro&a &a# m!#$a&i#!tra'

K!i'a#gam !'!tro#-!'!tro# &ari satu !'!tro&a &a# "!ro'!a# &ari !'!tro&a 'ai#,aa# m!#gasi'a# a'ira# !'!tro#-!'!tro# &a'am siruit !st!r#a' &ari a#o&a ! ato&a+!#ga# ata 'ai#, a#&a aa# m!m"!ro'! arus 'istri

 Arus %a#g &i"!ro'! ti&a *!sar, t!ta"i &a"at &i"!ruat 2ia s!#%aa-s!#%aaorga#i '!*i *a#%a &a'am #%a'a, maa aa# *a#%a $uga &iasi'a# io#-io#, &a# &!#ga#&!miia# aa# t!r$a&i arus 'istri %a#g '!*i uat I#i a&a'a "!#&!ata# %a#g *!ra'asa#,usus#%a $a a#&a *!r*icara t!#ta#g s!#%aa-s!#%aa %a#g s!ru"a, arus %a#g a#&auur s!*a#&i#g &!#ga# $um'a s!#%aa &a'am #%a'a

K!ura#ga# utama &ari &!t!tor i#i a&a'a "!#grusaa# s!tia" asi' %a#g !'uar &ario'om s!*agaima#a %a#g t!r&!t!si 2ia a#&a aa# m!#grima# asi' ! s"!trom!t!r massa, misa'#%a u#tu a#a'isa 'a#$ut, a#&a ti&a &a"at m!#ggu#aa# &!t!tor ti"! i#i

*.  Pen+atat ",e+order$Fu#gsi r!cor&!r s!*agai a'at u#tu m!#c!ta asi' "!rco*aa# "a&a s!*ua !rtas

%a#g asi'#%a &is!*ut romatogram (um"u'a# "u#ca grafi)

Hasi' aa# &ir!am s!*agai uruta# "u#ca-"u#ca6 s!tia" "u#ca m!ai'i satus!#%aa &a'am cam"ura# %a#g m!'a'ui &!t!tor S!"a#$a#g a#&a m!#go#tro' s!cara ati-ati o#&isi &a'am o'om, a#&a &a"at m!#ggu#aa# atu r!t!#si u#tu m!m*a#tum!#gi&!#tifiasi s!#%aa %a#g tam"a-t!#tu sa$a a#&a atau s!s!ora#g 'ai# t!'am!#ga#a'isa s!#%aa mur#i &ari *!r*agai s!#%aa "a&a o#&isi %a#g sama

 Ar!a &i*aa "u#ca s!*a#&i#g &!#ga# $um'a s!tia" s!#%aa %a#g t!'am!'!ati &!t!tor, &a# ar!a i#i &a"at &iitu#g s!cara otomatis m!'a'ui om"ut!r %a#g&iu*u#ga# &!#ga# mo#itor Ar!a %a#g aa# &iuur tam"a s!*agai *agia# %a#g*!rar#a i$au &a'am gam*ar %a#g &is!&!ra#aa#

P!r'u &icatat *aa ti#ggi "u#ca ti&a m!ru"aa# masa'a, t!ta"i tota' ar!a&i*aa "u#ca +a'am *!*!ra"a co#to t!rt!#tu, *agia# iri gam*ar a&a'a "u#ca

t!rti#ggi &a# m!mi'ii ar!a %a#g "a'i#g 'uas Ha' i#i ti&a s!'a'u m!ru"aa# a' s!arus#%aMu#gi# sa$a s!$um'a *!sar satu s!#%aa &a"at tam"a, t!ta"i &a"at t!r*uti &ari

o'om &a'am $um'a r!'atif s!&iit m!'a'ui $um'a %a#g 'ama P!#guura# ar!a s!'ai# ti#ggi"u#ca &a"at &i"!rgu#aa# &a'am a' i#i

•  -aktu retensi

;atu %a#g &igu#aa# o'! s!#%aa t!rt!#tu u#tu *!rg!ra m!'a'ui o'om m!#u$u! &!t!tor &is!*ut s!*agi atu r!t!#si ;atu i#i &iuur *!r&asara# atu &ari saat

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sam"!' &ii#$!sia# "a&a titi &ima#a tam"i'a# m!#u#u$ua# ti#ggi "u#ca masimumu#tu s!#%aa itu

S!tia" s!#%aa m!mi'ii atu r!t!#si %a#g *!r*!&a <#tu s!#%aa t!rt!#tu,atu r!t!#si sa#gat *!r1ariasi &a# *!rga#tu#g "a&a Titi &i&i s!#%aa S!#%aa %a#gm!#&i&i "a&a t!m"!ratur %a#g '!*i ti#ggi &ari"a&a t!m"!ratur o'om, aa#m!#ga*isa# am"ir s!'uru atu#%a u#tu *!ro#&!#sasi s!*agai caira# "a&a aa'o'om +!#ga# &!miia#, titi &i&i %a#g ti#ggi aa# m!mi'ii atu r!t!#si %a#g 'ama

K!'aruta# &a'am fas! cair S!#%aa %a#g '!*i mu&a 'arut &a'am fas! cair, aa#m!m"u#%ai atu '!*i si#gat u#tu &i*aa o'! gas "!m*aa K!'aruta# %a#g ti#ggi&a'am fas! cair *!rarti m!miii atu r!t!#si %a#g 'ama

T!m"!ratur o'om T!m"!ratur ti#ggi m!#%!*aa# "!rg!raa# mo'!u'-mo'!u'&a'am fas! gas6 *ai ar!#a mo'!u'-mo'!u' '!*i mu&a m!#gua", atau ar!#a !#!rgiatrasi %a#g ti#ggi caira# &a# o'! ar!#a itu ti&a 'ama t!rtam*ata# T!m"!ratur o'om%a#g ti#ggi m!m"!rsi#gat atu r!t!#si u#tu s!ga'a s!suatu#%a &i &a'am o'om

<#tu m!m*!ria# sam"!' &a# o'om, ti&a a&a *a#%a %a#g *isa &i!r$aa#m!#ggu#aa# titi &i&i s!#%aa atau !'aruta##%a &a'am fas! cair, t!ta"i a#&a &a"atm!m"u#%ai "!#gatur t!m"!ratur

S!mai# r!#&a t!m"!ratur o'om s!mai# *ai "!misaa# %a#g aa# a#&a&a"ata#, t!ta"i aa# m!maa# atu %a#g 'ama u#tu m!#&a"ata# s!#%aa ar!#ao#&!#sasi %a#g 'ama "a&a *agia# aa' o'om

+!#ga# ata 'ai#, m!#ggu#aa# t!m"!ratur ti#ggi, s!ga'a s!suatu#%a aa# m!'a'uio'om '!*i c!"at, t!ta"i "!misia##%a ura#g *ai 2ia s!ga'a s!suatu#%a m!'a'ui o'om&a'am atu %a#g sa#gat si#gat, ti&a aa# t!r&a"at $ara a#tara "u#ca-"u#ca &a'amromatogram

2aa*a##%a &imu'ai &!#ga# o'om &!#ga# suu %a#g r!#&a !mu&ia# "!r'aa#-'aa# s!cara t!ratur t!m"!ratur#%a &i#aia#Pa&a aa'#%a, s!#%aa %a#g m!#ga*isa# '!*i *a#%a atu#%a &a'am fas! gas aa#m!'a'ui o'om s!cara c!"at &a# &a"at &i&!t!si +!#ga# a&a#%a s!&iit "!rtam*aa#t!m"!ratur aa# m!m"!r$!'as "!r'!ata# s!#%aa P!#i#gata# t!m"!ratur masi &a"at

'!*i =m!'!ata#= mo'!u'-mo'!u' fas! &iam m!'a'ui o'om

). 

Penerapan kromatografi gas7  <#tu i&!#tifiasi s!#%aa

+!#ga# suatu o'om t!rt!#tu &a# &!#ga# s!mua faria*!'#%a s!"!rti t!m"!ratur &a# 'a$ua'ir, &i!#&a'ia# s!cara c!rmat, atu r!t!#si atau 1o'um! r!t!#si suatu .at t!r'arutm!ru"aa# suatu *!sara# &ari .at t!r'arut t!rs!*ut, s!"!rti a'#%a tit &i&i atau a'#%ai#&! *ias a&a'a *!sara# I#i m!#u#$ua# *aa sifat r!t!#si &a"at &igu#aa# u#tum!#g!taui suatu s!#%aa

5 

 A#a'isis ua#titatif +!#ga# GC t!rga#tu#g "a&a u*u#ga# a#tara $um'a suatu .at t!r'arut &a# uura# &ari

"ita !'usi %a#g &iasi'a# S!cara umum &!#ga# &!t!tor &if!r!#sia', uura# $um'a .att!r'arut %a#g "a'i#g *ai a&a'a 'uas &i*aa "ita !'usi 2um'a .at t!r'arut > fator a'i*rasi? 'uas &i*aa "ita !'usi

K!t!r*asa# GC a&a'a 1o'ati'itas sam"!' itu arus m!m"u#%ai t!a#a# ua" %a#gcuu" "a&a t!m"!ratur o'om t!rs!*ut, &a# i#i s!g!ra m!#gi'a#ga# *a#%a $!#is sam"!'Suatu "!ritu#ga# %a#g atua' ti&a mu#gi# &i'aua# t!ta"i arus &i"!riraa# *aas!itar 5:@ s!#%aa imia %a#g &i!taui ura#g cuu" 1o'ati'!*a#%aa# sam"!' orga#iti&a cuu" 1o'ati' u#tu m!mu#gi#a# "!#!ra"a# 'a#gsu#g &ari GC

7/21/2019 Kromatografi Gas

http://slidepdf.com/reader/full/kromatografi-gas-56d9b2f3ca5aa 7/7

.  CA,A K,/A K,0MAT0G,A& GA7  M!#cuci $arum su#ti &!#ga# as!to# &!#ga# m!#gisi $arum su#ti m!#&!"a s!"!#u#%a

&a# as!to# 'im*a ! !rtas a#&u Cuci 5- a'i5  Tari *!*!ra"a sam"!' A#&a ! &a'am $arum su#ti A#&a mu#gi# "!r'u u#tu

m!#gi'a#ga# g!'!m*u#g u&ara &i &a'am ta*u#g su#ti o'! "'u#%!r *!rg!ra c!"at !atas &a# ! *aa s!m!#tara $arum &a'am sam"!' /iasa#%a 7-5 mL sam"!' &isu#tia#! &a'am GC /o'! sa$a m!mi'ii g!'!m*u#g u&ara !ci' &a'am $arum su#ti 3amu#, A#&ati&a i#gi# m!#%u#tia# s!*agia# *!sar u&ara atau "u#ca A#&a aa# t!r'a'u !ci' "a&ata*!' "!r!am

 

Pastia# ta*!' "!r!am &a# &iatur ! !c!"ata# grafi %a#g s!suai (Arro A) M!#gatur *as!'i#! m!#ggu#aa# #o' "a&a ta*!' "!r!am (Arro /) +!#ga# "!#a &i t!m"at,m!#%a'aa# *aga# (Arro +), "astia# "!#a ! *aa (%a#g m!#a#&ai !rtas) &a# !rtas*!rg!ra

8  M!#%u#tia# sam"!' A#&a *ai ! o'om A atau o'om / s!suai i#strusi P!ga#g ti#gat $arum su#ti &a# m!#&oro#g $arum s!"!#u#%a ! i#$!ctor S!t!'a A#&a ti&a &a"at 'agim!'iat $arum, &!#ga# c!"at m!#&oro#g "!#&oro#g &a# !mu&ia# tari $arum su#ti i#$!si!'uar &ari "!'a*ua#

I#$!si Catata# i#$!ctor sa#gat "a#as, $a&i *!rati-ati'a u#tu ti&a m!#%!#tu "!ra&is 2arum aa# m!'!ati s!"tum ar!t, s!i#gga A#&a aa# m!rasa *!*!ra"a"!r'aa#a# <#tu *!*!ra"a GC ita itu, o'om ti&a m!#%!'arasa# *!#ar &a'am i#$!ctor,s!i#gga $arum its *agia# &!"a# o'om 'ogam 2ia A#&a m!rasa *aa A#&a m!#&oro#gt!ra&a" 'ogam, m!#ari $arum !'uar &ari i#$!ctor &a# co*a 'agi, mu#gi# &i su&ut %a#gs!&iit *!r*!&a 2arum arus *!#ar-*!#ar m!#gi'a#g ! &a'am i#$!si u#tu i#$!si %a#gt!"at sam"!' ! o'om GCSu#tia# &!#ga# c!"at u#tu asi' t!r*ai 2a#ga# ragu u#tum!#%u#tia# $arum s!t!'a *!#ar &i"osisia# &i "!'a*ua# i#$!siL!"asa# $arum su#tis!g!ra s!t!'a i#$!si (P!'asa#aa# catata# C &a# + m!m*a#tu u#tu m!mastia# *aas!mua sam"!' m!masui GC o'om &i s!itar atu %a#g sama)

9  M!#a#&ai atu i#$!si A#&a "a&a ta*!' "!r!am I#i &a"at &i'aua# &!#ga#

m!#%!suaia# #o' t!"at s!t!'a sam"!' &isu#tia# Ha' i#i s!ri#g #%ama# *agi satu ora#gu#tu m!#%u#tia# sam"!' s!m!#tara "asa#ga# 'a*oratorium m!#a#&ai atu i#$!si &i*aga# "!r!am

B  /!rsia# $arum su#ti A#&a s!g!ra s!t!'a i#$!si 2arum su#ti s!ri#g t!rsum*at &!#ga#c!"at &a# arus &iga#ti $ia m!r!a ti&a &i*!rsia# s!t!'a s!tia" "!#ggu#aa#

  Catata# "!#gatura# "!r!am grafi A#&a s!'ama *!r$a'a# A#&a "!r'u m!#g!taui!c!"ata# grafi &a# "!#gatura# sa'a "!#u

D  Catata# "!#gatura# GC s!'ama A#&a *!r'ari S!*ua tom*o' &i *agia# t!#ga *aa GC&a"at &iu*a u#tu m!m*aca o'om (atau o1!#) suu, suu &!t!tor &a# suu i#$!tor "!'a*ua# &a'am E C 2!m*ata# saat i#i &itam"i'a# &a'am mA P!ratia# *aa a&a &uasa'a "a&a 'a%ar /!rati-ati u#tu m!m*aca sa'a %a#g t!"at

. 

AMPL 2A3G )APAT )&A3AL&& )3GA3 GCSam"!' %a#g &a"at &ia#a'isis &!#ga# GC &ia#tara#%a a&a'a 7

 

Pro&u Gas A'am5  K!mur#ia# P!'arut   Asam L!ma