Data Pengamatan Dan Pengolahan Data Kematian Mikroba Batch

7/25/2019 Data Pengamatan Dan Pengolahan Data Kematian Mikroba Batch

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DATA PENGAMATAN DAN PENGOLAHAN DATA

4.1 Data Pengamatan

Jumlah Sel Hidup ,Sel Mati dan Sel Total Pada Sterilisasi Batch

T1= 50oC

Waktu (menit)

Jumlah sel

Hidup Mati Total

I II I II I II

t0 0 82 90 28 0 110 120

t1 5 12 !9 2" 15 159 8#

t2 10 82 "! 5 50 11" 12!

t 15 102 !2 52 #9 15# 111

t# 20 10 90 11 1# 1#1 10#

T2= !0oC

Waktu (menit)

Jumlah sel

Hidup Mati Total

I II I II I II

t0 0 2" # 2 5 29 9

t1 5 202 21# # 2 20! 21!

t2 10 ! 10 10! 8# 112 9#

t 15 0 28 8# !! 8# 9#

t# 20 22 1 0 0 22 1

T= "0oC


2/14

Waktu (menit)

Jumlah sel

Hidup Mati Total

I II I II I II

t0 0 5! 5# 2 2 58 5!

t1 5 2 ! 0 0 2 !

t2 10 0 28 ! !2 9 90

t 15 5 1# 15! 19 159

t# 20 1!2 1# 51 #9 21 192

4.2 Pengolahan Data

Perhitungan lnNt

N ountuk variasi suhu ere!a

$ete%an&an ' t = %ata%ata hidup

o= %ata%ata hidup * %ata%ata mati

T1= 50oC

t" # " menit $$$ %LnNt

No# Ln

(82+90)/2(82+90)/2+(28+30) /2

# ln86

115#

$"&2'

t2 # 1&( menit$$$ %LnNt

No# Ln

(132+69)/2(132+69)/2+(27+15)/2

# ln100,5

121 ,5

# $"&1)'

t*# * menit $$$ %LnNt

No# Ln

(82+76)/2

(82+76)/2+(35+50)/2 # ln

79

121,5

# $"&4*

t4 # 4&( menit$$$ %LnNt

No# Ln

(102+62)/2(102+62) /2+(52+49)/2

# ln82

132,5#

$"&4+'


3/14

t4 # , menit $$$ %LnNt

No# Ln

(130+90)/2(130+90)/2+(11+14)/2

# ln110

122 ,5#

$"&1"+

T2= 60oC


No# Ln

(27+34 )/2(27+34) /2+(2+5)/2

# ln30 ,5

34#

$"&1")

t1 # ( menit $$$ %LnNt

No# Ln

(202+214 )/2(202+214)/2+(4+2)/2

# ln208

211#

$"&"14*

t2 # 1" menit $$$ %LnNt

N o# Ln

(6+10)/2(6+10)/2+(106+84)/2

# ln8

103 #

$2&((

t*# 1( menit $$$ %LnNt

No# Ln

(0+28)/2(0+28)/2+(84+66)/2

# ln14

89 #

$1&)4'

t4 # 2" menit $$$ %Ln

Nt

No # Ln

(22+31)/2

(22+31)/2+(0+0)/2 # ln

26 ,5

26 ,5 #

"

T*# +"o-


No# Ln

(56+54)/2(56+54)/2+(2+2)/2

# ln55

57#

$"&"*(+

t1 # ( menit $$$ %LnNt

No# Ln

(32+36)/2(32+36)/2+(0+0)/2

# ln34

34# "

t2 # 1" menit $$$ %LnNt

No# Ln

(30+28)/2(30+28)/2+(63+62)/2

# ln29

62 ,5

# $"&+,+


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t*# 1( menit $$$ %LnNt

No# Ln

(5+3)/2(5+3)/2+(134+156)/2

# ln4

149

# $*&,1+

t4 # 2" menit $$$ %Ln

Nt

No # Ln

(162+143)/2

(162+143)/2+(51+49)/2 # ln

152 ,5

202 ,5 # $"&2)*

+%a,ik -nNt

Noterha!a t /0aktu untuk variasi suhu ere!a

T1= 50oC

0 5 10 15 20 25

-0.6

-0.5

-0.4

-0.3

-0.2

-0.1

0

f(x) = 0x - 0.31

R = 0.01

Kinetika Kematian Mikroba 50o

Linear ()

t (menit)

ln (lt/lo)

.e%hitun&an $d'

/ = a *

= 030015 031#2

4e%dasa%kan pe%samaan LnNt

No# $ ! . t 3 maka'


5/14

! # $/$"&""1( # "&""1(

T2= 60oC

0 5 10 15 20 25

-3

-2.5

-2

-1.5

-1

-0.5

0

f(x) = - 0.03x - 0.58

R = 0.04

Kinetika Kematian Mikroba pada 60o

Linear ()

Axis Title

Axis Title

.e%hitun&an $d'

= 0302# 035805


No# $ ! . t3 maka'

! # $/$"&"*24#"&"*24

T*# +"o-


6/14

0 5 10 15 20 25

-4

-3.5

-3

-2.5

-2

-1.5

-1

-0.5

0

f(x) = - 0.08x - 0.12

R = 0.18

Kinematika Kematian Mikroba 70o

Linear ()

Axis Title

Axis Title

.e%hitun&an $d'

= 030822 031182


No# $ ! . t 3 maka'

! # $/$"&")22 #"&")22

Tael !& ln !& !an 13T untuk terilisasi 5at6h

G

ra7ik ln ! terha!a 13T untuk terilisasi 5at6h

T /temerature 13T ! Ln !

T1 = 50 oC 0302 030015 !3502

T2 = !0 oC 0301!" 0302# 3#29

T = "0 oC 0301#2 030822 23#98


7/14

0.010.010.020.020.020.020.020.020.02

-7

-6

-5

-4

-3

-2

-1

0

f(x) = - 702.87x + 7.78

R = 0.95

Chart Title

Linear ()

Axis Title

Axis Title

.e%hitun&an d'

= "0238" * "3"82

4e%dasa%kan pe%samaan '

ln kd=ln kd0Ed

RT

1

T

Maka3Ed

R= "3"82

d = "3"82 8,314 kJ / k!" #E! # $ ,4&+"2

467te%ilisasi Continue

.enentuan olume oil

:iamete% oil' 03 m

.an;an& oil' 535 m

alirasi La8u alir

o < 7kala pompa oulme (m-) Waktu (s)

1 !0 21 #5

2 "0 21 !

80 21


8/14

# 90 21 28

terilisasi -ontinuos

Tempe%atu%e penan&as (T1)' 50 C

o6 < 7kala .ompa Waktu (s)Jumlah sel

Hidup Mati Total

I II I II I II

16 !0 #8 15 20 25 2! #0 #!

26 "0 " 1# 1 8 11 22 2#

6 80 2 ## 28 2 2 #! 0

#6 90 2" 11" 1" 11 " 128 1##

Tempe%atu%e penan&as (T1)' !0 C


Hidup Mati Total

I II I II I II

16 !0 5 1"" 1#9 2! 52 20 201

26 "0 29 92 " ! " 98 ##

6 80 28 51 #0 ! ! 5" #!

#6 90 2! 120 98 2# # 1## 12

Tempe%atu%e penan&as (T1)' "0 C


Hidup Mati Total

I II I II I II

16 !0 #0 1 !8 "2 "1 "

26 "0 # 2 1 9 10" 95 108

6 80 28 2 0 21 2#" 215 2#"

#6 90 2" # 2 8" "5 91 ""


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PENGOLAHAN DATA

Perhitungan 9 /saat kalirasi

!0 < = 21 m-t = #5 detik

> =V

t

=21

45= "&4,+ mL3!etik

"0 < = 21 m-t = ! detik

> =V

t

=21

36 = "&()* mL3!etik

80 < = 21 m-t = detik

> =V

t

=21

33= "&,*, mL3!etik

90 < = 21 m-t = 28 detik

> =V

t

=21

18= "&+( mL3!etik

Perhitungan volume ia 6oil

Tin&&i = 535 m

:iamete% = 03 m

Jumlah lilitan = 22

.an;an& lilitan = x tinggi x diameter

= 31# 535 22 = "939# m2

olume oil = luas alas pan;an& lilitan= (03152 31#) "939#

= 2!38# m

Perhitungan 0aktu tinggal

t #volume pipa

Q(lajualir )


10/14

t1=V

Q=

21

0,467= ##39! detik

t2=V

Q=

21

0,583= !302 detik

t=V

Q

=21

0,636

= 301 detik

t#=V

Q=

21

0,75= 28 detik

: Menghitung nilai kd

- Tempe%atu% penan&as (T) ' 50oC

Waktu tin&&al t o -nNt

No

##39! 1"35 # 03898!302 135 2 0352

301 ! 8 0305#

28 12" 1! 030!8

$et ' 0= ;umlah mik%oa hidup

t= ;umlah mik%oa total setelah pemanasan (pe%iode t )

- Tempe%atu% penan&as (T) ' !0o

C

Waktu tin&&al t o -nNt

No

##39! 1 1"2 0325"

!302 !#35 "1 0309!

301 #535 5135 0312

28 109 18 0325




11/14

- Tempe%atu% penan&as (T) ' "0oC

Waktu tin&&al t o -n NtNo

##39! 2 "2 358

!302 135 10135 #321#

301 1 20 53#8

28 8# 32



Gra7ik LnNt

Noterha!a /0aktu untuk T # ("o-

26 28 30 32 34 36 38 40 42 44 46

-1

-0.8

-0.6

-0.4

-0.2

0f(x) = - 0.05x + 1.51

R = 0.88

Grafk ln (t/o) thd t 50o

Linear ()

t (s)

ln (t/o)

Perhitungan !;

= 0305# * 13508


No# $ ! . t3 maka'

! # $/$"&"(*4 # "&"(*4


12/14

Gra7ik LnNt

Noterha!a /0aktu untuk T # ,"o-

26 28 30 32 34 36 38 40 42 44 46

-0.3

-0.25

-0.2

-0.15

-0.1

-0.05

0

f(x) = - 0x - 0.08

R = 0.06


Linear ()

Axis Title

Axis Title

Perhitungan !;

= 03002" 030812


No# $ ! . t3 maka'

! # $/$"&""2+ # "&""2+

Gra7ik Ln NtNo

terha!a /0aktu untuk T # +"o-

26 28 30 32 34 36 38 40 42 44 46

-6

-5

-4

-3

-2

-1

0

f(x) = 0.02x - 4.7

R = 0.01


Linear ()

Axis Title

Axis Title


13/14

Perhitungan !;

= 030158 #3"021


No# $ ! . t3 maka'

! # $/$"&"1() # "&"1()

Tael !& Ln !& !an 13T untuk terilisasi -ontinue

! Ln ! 13T

0305# 23929 0302

03002" 5391# 0301!"

030158 #31#" 0301#2

Gra7ik ln ! terha!a 13T untuk terilisasi -ontinuos

0.010.010.020.020.020.020.020.020.02

-7-6

-5

-4

-3

-2

-1

0

f(x) = 246.11x - 8.51

R = 0.23

Chart Title

Linear ()

Axis Title

Axis Title

.e%hitun&an d'

= 2#!311 83505!


14/14

4e%dasa%kan pe%samaan '

ln kd=ln kd0Ed

RT

1

T

Maka3Ed

R

= 83505!

d = 83505! 8,314 kJ / k!" #

E! # +"&+1( kalue /D

D #ln10

Kd

DT1#ln10

0,0534# 4*&1")

DT2#ln10

0,0027# )(2&('

DT* #ln10

0,0158# 14(&,',

D /Destru6tion >alue

D1 4*&1")

D2 )(2&('

D* 14(&,',

Data Pengamatan Dan Pengolahan Data Kematian Mikroba Batch

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