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NUCLEIC ACIDELECTROPHORESIS
Horizontal Electrophoresis Selection Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
HE33 Mini Horizontal Agarose Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . . . 34
HE33 Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
HE99X Max Horizontal Agarose Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . . 36
HE99X Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
SUB6 Mini Submarine Gel Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
SUB6 Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
SUB10 Mini-Plus Submarine Gel Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . 40
SUB10 Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
SUB13 Midi Submarine Gel Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
SUB13 Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
SUB15 Standard Submarine Gel Electrophoresis Unit . . . . . . . . . . . . . . . . . . . . . . . . 44
SUB15 Accessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
SUB20 Maxi-Standard and SUB20C Maxi-CooledSubmarine Gel Electrophoresis Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
SUB20 Maxi-Standard and SUB20C Maxi-CooledAccessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
SUB25 Maxi-Plus Standard and SUB25C Maxi-Plus CooledSubmarine Gel Electrophoresis Units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
SUB25 and SUB25C Maxi-Plus Standard and CooledAccessories and Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
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Horizontal ElectrophoresisSelection Guide
Product Page # Tray Size Maximum CoolingName (w x l cm) Sample Capacity OptionHE33 34 7 x 10 32 YesHE99X 36 15 x 10 30 –
15 x 15 6015 x 20 60
SUB6 38 6 x 7.5 32 –SUB10 40 10 x 11.5 80 –SUB13 42 12.8 x 15 112 –SUB15 44 15 x 15 120 –SUB20 46 20 x 20 160 –SUB20C 46 20 x 20 160 YesSUB25 48 25 x 30 624 –SUB25C 48 25 x 30 624 Yes
The recommended power supply for the above horizontal units is the Hoefer PS300B(see page 64).
Hoefer, the premier manufacturer of electrophoresis equipment, has expandedits family of horizontal electrophoresis units providing a solution for almostevery nucleic acid application.
Hoefer’s HE33 and HE99X classic horizontals have been used in electrophoresislaboratories around the world for more than 10 years.
Hoefer is pleased to introduce a new line of submarine units which offers eightmodels from mini units to large cooled units in a new contemporary design.
Classic
Con
tempo
rary
Toll Free: (800) 227-4750 • Tel: +1 (508) 893-8999E-mail: sales@hoeferinc.com •Web: www.hoeferinc.com
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HE33 Mini HorizontalAgarose Electrophoresis UnitPerform rapid separation of DNA fragments
The HE33 Mini Horizontal Agarose Unit is designed for very fastseparations of DNA restriction fragments in agarose gels. To achievepassive cooling, you simply fill the clear molded base with 50%ethylene glycol coolant solution, seal it, and place it in a freezer or
ice bath until ready to use.
Technical SpecificationsGel Dimensions (w x l) . . . . . . . 7 x 10 cmMaximum Buffer Volume . . . . . . 250 mlMaximum Power Settings . . . . . . 500 V for (5 min or less), 500 mA, 15 WMaximum Sample Capacity . . . . 32Maximum Temperature . . . . . . . 50°CIndoor Use . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (l x h x w) . . . . 24 x 7 x 13 cmSafety Certifications . . . . . . . . . . EN61010-1, UL61010A-1, CSA C22.2 1010.1, CE
Ordering InformationCat. # DescriptionHE33-8-1.5 HE33 Mini Horizontal Unit, Complete
Cat. # DescriptionHE33B HE33 Mini Horizontal Unit, Basic
Complete Unit Includes:• Buffer Chamber Assembly • Foam Gaskets–4 pcs• Safety Lid w/High Voltage Leads • Bubble Level• Running Tray • 1.5 mm thick, 8 well comb• Casting Tray • Comb Back
Order HE31A combs and HE31-BK comb backs separately, see page 35.
Basic Unit Includes:• Buffer Chamber Assembly • Casting Tray• Safety Lid w/High Voltage Leads • Foam Gaskets–4 pcs• Running Tray • Bubble Level
Features and BenefitsFast separations–run a 7 x 10 cm gelin as little as 5 minutes
UV transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling
Variety of comb sizes–enablescustomization of samplethroughput to fit your laboratoriesrequirements
Adjustable comb height–completecontrol over well depth
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HE33 CombsCat. # # of Wells Thickness Well Width Sample Volume in a
(mm) (mm) 5 mm DeepWell (µl)HE31A-P-1.0 1/2* 1.0 44/6 235/35HE31A-P-1.5 1/2* 1.5 44/6 355/50HE31A-8-1.0 8 1.0 6.5 32.5HE31A-8-1.5 8 1.5 6.5 48.5HE31A-12-1.0 12 1.0 3.9 19.5HE31A-12-1.5 12 1.5 3.9 30.5HE31A-16-1.0 16 1.0 2.6 13HE31A-16-1.5 16 1.5 2.6 20.5
HE33 Mini HorizontalAccessories and Replacement Parts
Accessories and Replacement PartsCat. # DescriptionHE47-10 Gel Casting Kit:
7 x 10 cm Running Tray7 x 10 cm Casting TrayFoam Gaskets–4 pcs
HE42-10 7 x 10 cm Running TrayHE45-10 7 x 10 cm Casting TraySER11 Bubble LevelHE48 Foam Gaskets–4 pcsHE38TP Top Fill Plug Kit–4 pcsHE31-BK Comb Back + 2 screwsHE30 Buffer Chamber AssemblyHE36 Lid w/High Voltage LeadsSE6056-HV High Voltage Leads–2 pcsHE39 Electrode Replacement Kit
HE47-10
HE42-10
HE45-10
HE38TP
SER11
*1 preparatory and 2 reference wellsAll combs require HE31-BK comb back ordered separately
HE36HE30 HE39
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HE99X Max HorizontalAgarose Electrophoresis Unit
Offers a variety of gel lengths and comb sizesto enable the design of the gel required foroptimum resolution of DNA fragments
The HE99X Max agarose electrophoresis unit facilitates highresolution separation of nucleic acid fragments 100 bp to 20 kbp.Three different length trays are available for use with this unit.
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . . . . . 15 x 20 cm, 15 x 15 cm, or 15 x 10 cmMaximum Buffer Volume . . . . . . . . . . . . . . 1.2 LMaximum Power Settings . . . . . . . . . . . . . . 200 V, 100 mA, 20 WMaximum Sample Capacity . . . . . . . . . . . . 60Maximum Temperature . . . . . . . . . . . . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (l x h x w) . . . . . . . . . . . . 36 x 14 x 18.2 cmSafety Certifications . . . . . . . . . . . . . . . . . . EN61010-1, UL61010A-1,
CSA C22.2 1010.1, CE
Ordering InformationCat. # DescriptionHE99X-15-1.5 HE99X Max Horizontal Unit, Complete
Complete Unit Includes:• Buffer Chamber Assembly • Foam Gaskets–4 pcs• Safety Lid w/High Voltage Leads • Bubble Level• Running Tray, 15 x 20 cm • 1.5 mm thick, 15 well comb• Casting Tray, 15 x 20 cm • Comb Back
Cat. # DescriptionHE99X HE99X Max Horizontal Unit, Basic
Basic Unit Includes:• Buffer Chamber Assembly• Safety Lid w/High Voltage Leads• Bubble LevelOrder a casting kit, HE91A combs and HE91-BK comb backs separately, see page 37.
Features and BenefitsSelf-locating/centering guides–supports consistent running trayplacement
Gel anchor feet–prevent the gelfrom floating or sliding duringthe run
Variety of comb sizes–enablescustomization of samplethroughput to fit your laboratoriesrequirements
Adjustable comb height–completecontrol over well depth
Three casting kits available–enabling users to run 15 x 20,15 x 15, or 15 x 10 cm gels in thesame unit
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HE99X CombsCat. # # of Wells Thickness Well Width Sample Volume in a
(mm) (mm) 5 mm DeepWell (µl)HE91A-P-1.5 1/2* 1.5 113/10 855/72.5HE91A-P-3.0 1/2* 3.0 113/10 1710/145.5HE91A-10-1.5 10 1.5 9.7 72.5HE91A-10-3.0 10 3.0 9.7 145.5HE91A-15-1.0 15 1.0 7.1 35.5HE91A-15-1.5 15 1.5 7.1 53.5HE91A-15-3.0 15 3.0 7.1 106.5HE91A-20-1.0 20 1.0 4.7 23.5HE91A-20-1.5 20 1.5 4.7 35.5HE91A-20-3.0 20 3.0 4.7 70.5HE91A-30-1.0 30 1.0 3.0 15
Accessories and Replacement PartsCat. # DescriptionGel Casting Kit (Running Tray, Casting Tray, Foam Gaskets–4 pcs)HE97X-20 15 x 20 cmHE97X-15 15 x 15 cmHE97X-10 15 x 10 cmRunning TrayHE92X-20 15 x 20 cmHE92X-15 15 x 15 cmHE92X-10 15 x 10 cmCasting Tray (with Foam Gaskets–4 pcs)HE95X-20 15 x 20 cmHE95X-15 15 x 15 cmHE95X-10 15 x 10 cmHE91-BK Comb Back + 2 screwsSER11 Bubble LevelHE98X Foam Gaskets–4 pcsHE90X Buffer Chamber AssemblyHE96X Lid w/High Voltage LeadsSE6056-HV High Voltage Leads–2 pcsSE99XRK-1 Electrode Replacement Kit
Running Tray
Casting Tray
Gel Casting Kit
HE90X
SER11
HE91-BK
HE96X
HE99X Max HorizontalAccessories and Replacement Parts
*1 preparatory and 2 reference wellsAll combs require HE91-BK comb back ordered separately
HE99XRK-1
HE98X
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Features and BenefitsColor-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth
Colored loading strips–for easy welldetection when loading
Compact tank–reduces the buffervolume required to cover the gel,providing greater control over thevoltage gradient and run-time
UV-transparent running tray–allows the user to image the gelwithout risk of damage dueto handling
Side handles–for safe and easytransportation around thelaboratory
SUB6 Mini Submarine GelElectrophoresis UnitIdeal for routine preparatory and analyticalelectrophoresis techniques
Ordering InformationCat. # DescriptionSUB6 Mini Submarine Gel Unit
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 6 x 7.5 cmMaximum Buffer Volume . . . . . . . . . . 325 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 32Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 13 x 24 x 6.5 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE
Includes:• Buffer Chamber Assembly • Running Tray w/Silicone Gasket• Safety Lid • 1 mm thick, 8 well combs–2 pcs• High Voltage Leads • Colored Loading Strips
The SUB6 Mini Horizontal Gel Unit has a maximum32-sample throughput capacity and features a
removable gel-casting tray with end gaskets that allowgels to be cast directly within the tank.
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SUB6 In-Tank CastingThe included running tray is lined withsilicone gaskets which form a leak-proof seal against the inner walls of therunning chamber when the running trayis turned 90° to the direction ofelectrophoresis.
SUB6 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB6-C1-8 8 1 4.5 20SUB6-C1.5-8 8 1.5 4.5 30SUB6-C2-8 8 2 4.5 40SUB6-C1-12MC 12* 1 2.5 11SUB6-C1.5-12MC 12* 1.5 2.5 17SUB6-C2-12MC 12* 2 2.5 22SUB6-C1-16 16 1 2.2 10SUB6-C1.5-16 16 1.5 2.2 15SUB6-C2-16 16 2 2.2 20
Accessories and Replacement PartsCat. # DescriptionSUB6-TANK Buffer Chamber AssemblySUB6-LID Lid for SUB6SUB6-UT 6 x 7.5 cm Running Tray w/Silicone GasketSUB6-CS Colored Loading Strips–6 pcsSUB6-GS Gel Scoop for SUB6SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-FC External Adjustable Casting Unit
SUB6 Mini SubmarineAccessories and Replacement Parts
*Microtiter spacing for use with a multichannel pipette
SUB6-LID
Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays, increasingthroughput and efficiency by enablingthe tank to run concurrently.
SUB6-UT
SUB6-TANK
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SUB10 Mini-Plus Submarine GelElectrophoresis UnitIdeal for routine preparatory and analyticalelectrophoresis techniques
Features and BenefitsColor-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth
Colored loading strips–for easy welldetection when loading
Compact tank–reduces the buffervolume required to cover the gel,providing greater control over thevoltage gradient and run-time
UV-transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling
Side handles–for safe and easytransportation around the laboratory
Ordering InformationCat. # DescriptionSUB10 Mini-Plus Submarine Gel Unit
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 10 x 11.5 cmMaximum Buffer Volume . . . . . . . . . 450 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 80Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . 16.5 x 23 x 6.5 cmSafety Certifications . . . . . . . . . . . . . EN61010-1, CE
Includes:• Buffer Chamber Assembly • Running Tray w/Silicone Gasket• Safety Lid • 1 mm thick, 16 well combs–2 pcs• High Voltage Leads • Colored Loading Strips
The SUB10 Mini-Plus Horizontal Gel Unit has a maximum80-sample throughput capacity and features a removable gel-
casting tray with end gaskets that allow gels to be cast directlywithin the tank.
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Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays, increasingthroughput and efficiency by enablingthe tank to run concurrently.
SUB10 In-Tank CastingThe included running tray is lined withsilicone gaskets which form a leak-proof seal against the inner walls of therunning chamber when the running trayis turned 90° to the direction ofelectrophoresis.
Accessories and Replacement PartsCat. # DescriptionSUB10-TANK Buffer Chamber AssemblySUB10-LID Lid for SUB10SUB10-UT 10 x 11.5 cm Running Tray w/Silicone GasketSUB10-CS Colored Loading Strips–6 pcsSUB10-GS Gel Scoop for SUB10SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-FC External Adjustable Casting Unit
SUB10 Mini-Plus SubmarineAccessories and Replacement Parts
SUB10 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB10-C1-8 8 1 9 40SUB10-C1.5-8 8 1.5 9 60SUB10-C2-8 8 2 9 80SUB10-C1-10MC 10* 1 7 30SUB10-C1.5-10MC 10* 1.5 7 45SUB10-C2-10MC 10* 2 7 60SUB10-C1-12 12 1 5.5 25SUB10-C1.5-12 12 1.5 5.5 35SUB10-C2-12 12 2 5.5 50SUB10-C1-16 16 1 3.6 15SUB10-C1.5-16 16 1.5 3.6 25SUB10-C2-16 16 2 3.6 30SUB10-C1-20MC 20* 1 3 12SUB10-C1.5-20MC 20* 1.5 3 20SUB10-C2-20MC 20* 2 3 25
*Microtiter spacing for use with a multichannel pipette
SUB10-LID
SUB10-TANK
SUB10-UT
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SUB13 Midi Submarine GelElectrophoresis Unit
Ideal for analytical and preparative studiesof nucleic acids
Features and BenefitsColor-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth
Buffer recirculation ports–may beconnected to a peristaltic pump(see page 141) for buffer recircu-lation during electrophoresis tomaintain buffer pH and preventionic gradient formation
Colored loading strips–for easy welldetection when loading
Compact tank–reduces the buffervolume required to cover the gel,providing greater control over thevoltage gradient and run-time
UV-transparent running tray– allowsthe user to image the gel without riskof damage due to handling
Side handles–for safe and easy trans-portation around the laboratory
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 12.8 x 15 cmMaximum Buffer Volume . . . . . . . . . 900 mlMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 112Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 20 x 32 x 7 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE
Ordering InformationCat. # DescriptionSUB13 Midi Submarine Gel Unit
Includes:• Buffer Chamber Assembly • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Silicone Gasket
The SUB13 Midi Submarine Gel Unit has a maximum112-sample throughput capacity and features a removable
gel-casting tray with end gaskets that allow gels to be castdirectly within the tank.
Toll Free: (800) 227-4750 • Tel: +1 (508) 893-8999E-mail: sales@hoeferinc.com •Web: www.hoeferinc.com
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SUB13 In-Tank CastingThe included running tray is lined withsilicone gaskets which form a leak-proof seal against the inner walls of therunning chamber when the running trayis turned 90° to the direction ofelectrophoresis.
Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays, increasingthroughput and efficiency by enablingthe tank to run concurrently.
Accessories and Replacement PartsCat. # DescriptionSUB13-TANK Buffer Chamber AssemblySUB13-LID Lid for SUB13SUB13-UT 12.8 x 15 cm Running Tray w/Silicone GasketSUB13-CS Colored Loading Strips–6 pcsSUB13-GS Gel Scoop for SUB13SUB-LEAD High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcsSUB-FC External Adjustable Casting Unit
SUB13 Midi SubmarineAccessories and Replacement PartsSUB13 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB13-C1-10 10 1 9.5 40SUB13-C1.5-10 10 1.5 9.5 60SUB13-C2-10 10 2 9.5 85SUB13-C1-12MC 12* 1 8 35SUB13-C1.5-12MC 12* 1.5 8 50SUB13-C2-12MC 12* 2 8 70SUB13-C1-16 16 1 5.5 25SUB13-C1.5-16 16 1.5 5.5 35SUB13-C2-16 16 2 5.5 50SUB13-C1-20 20 1 4 17SUB13-C1.5-20 20 1.5 4 25SUB13-C2-20 20 2 4 35SUB13-C1-24 24 1 3 13SUB13-C1.5-24 24 1.5 3 20SUB13-C2-24 24 2 3 25SUB13-C1-28MC 28* 1 3 13SUB13-C1.5-28MC 28* 1.5 3 20SUB13-C2-28MC 28* 2 3 25
*Microtiter spacing for use with a multichannel pipette
SUB13-UT
SUB13-LID
SUB13-TANK
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SUB15 Standard Submarine GelElectrophoresis Unit
Provides the most commonly used tray sizeand comb options
Features and BenefitsThe market standard–a 15 x 15 cm geltray, with comb options ranging from1 to 30 samples, is currently the mostpopular format in today’s market
Multichannel pipette compatiblecombs–with a maximum 30 samplethroughput reduce gel loading time,while preparatory combs enablenucleic acids to be scaled-up forcloning
Color-coded and height adjustablecombs–easily identify comb thicknessat a glance and control well depth
Buffer recirculation ports–may beconnected to a peristaltic pump(see page 141) for buffer recirculationduring electrophoresis to maintainbuffer pH and prevent ionicgradient formation
Colored loading strips–for easy welldetection when loading
UV-transparent running tray–allowsthe user to image the gel without riskof damage due to handling
Side handles–for safe and easy trans-portation around the laboratory
Ordering InformationCat. # DescriptionSUB15 Standard Submarine Gel Unit
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 15 x 15 cmMaximum Buffer Volume . . . . . . . . . 1.2 LMaximum Power Settings . . . . . . . . . 300 V, 200 mA, 60 WMaximum Sample Capacity . . . . . . . 120Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . 21.5 x 33.5 x 7cmSafety Certifications . . . . . . . . . . . . . EN61010-1, CE
Includes:• Buffer Chamber Assembly • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates
A maximum 120-sample throughput makes the SUB15Standard Gel Unit a highly versatile option for screening
samples ranging from DNA mini-preps to PCR products.
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*Microtiter spacing for use with a multichannel pipette
Accessories and Replacement PartsCat. # DescriptionSUB15-TANK Buffer Chamber AssemblySUB15-LID Lid for SUB15SUB15-UT 15 x 15 cm Running Tray w/Casting GatesSUB15-CG Casting Gates w/Silicone Gasket–2 pcsSUB15-CS Colored Loading Strips–6 pcsSUB15-GS Gel Scoop for SUB15SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcsSUB-FC External Adjustable Casting Unit
SUB15 Standard SubmarineAccessories and Replacement Parts
SUB15 Casting GatesThe included casting gates conven-iently and efficiently seal the runningtray without the hassles of using tape.
SUB15 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB15-C1-10 10 1 12.8 64SUB15-C1.5-10 10 1.5 12.8 96SUB15-C2-10 10 2 12.8 128SUB15-C1-16MC 16* 1 6.4 32SUB15-C1.5-16MC 16* 1.5 6.4 48SUB15-C2-16MC 16* 2 6.4 64SUB15-C1-20 20 1 5.9 29SUB15-C1.5-20 20 1.5 5.9 43SUB15-C2-20 20 2 5.9 58SUB15-C1-25 25 1 3.5 17SUB15-C1.5-25 25 1.5 3.5 25SUB15-C2-25 25 2 3.5 34SUB15-C1-30MC 30* 1 2.5 12SUB15-C1.5-30MC 30* 1.5 2.5 18SUB15-C2-30MC 30* 2 2.5 24
SUB15-LID
Optional External,Adjustable Casting UnitThe flexibility of the SUB-FC offers aconvenient external casting systemcapable of accommodating thecomplete range of Hoefer 6-15 cmwide running trays.
SUB15-UT
SUB15-TANK
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SUB20 Maxi-Standard andSUB20C Maxi-Cooled Submarine
Gel Electrophoresis UnitsIdeal for high resolution analytical andpreparative studies of nucleic acids
Features and BenefitsLarge format–20 x 20 cm gel tray isideal for high resolution techniques
Multichannel pipette compatiblecombs–with a maximum 40 samplethroughput–reduce gel loading time
Color-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth
Buffer recirculation ports–may beconnected to a peristaltic pump(see page 141) for buffer recircu-lation during electrophoresis tomaintain buffer pH and preventionic gradient formation
Colored loading strips–for easy welldetection when loading
UV-transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling
Side handles–for safe and easy trans-portation around the laboratory
Ordering InformationCat. # DescriptionSUB20 Maxi-Standard Submarine Gel Unit
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . 20 x 20 cmMaximum Buffer Volume . . . . . . . . . . 2.2 LMaximum Power Settings . . . . . . . . . 1000 V, 500 mA, 500 WMaximum Sample Capacity . . . . . . . 160Maximum Running Temperature . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . 27 x 47.5 x 8 cmSafety Certifications . . . . . . . . . . . . . . EN61010-1, CE
Includes:• Buffer Chamber Assembly • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates
The SUB20 Maxi-Standard Submarine Electrophoresis Unitprovides a 20 x 20 cm gel-running tray with four comb
positions which allows the user to perform high-resolution analyticresearch for a maximum of 168 samples. The SUB20C Maxi-CooledSubmarine Electrophoresis Unit has the same efficient design as the
SUB20 with the addition of a cooling base that can beconnected to a recirculation bath allowing high voltageelectrophoretic separations without overheating.
Cat. # DescriptionSUB20C Maxi-Standard Submarine Gel Unit w/Cooling Base
Includes:• Buffer Chamber Assembly w/Cooling Ports • 1 mm thick, 16 well combs–2 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates
SUB20
SUB20C
Toll Free: (800) 227-4750 • Tel: +1 (508) 893-8999E-mail: sales@hoeferinc.com •Web: www.hoeferinc.com
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Additional Features andBenefits of the SUB20CCooled base–covering the entire20 x 20 cm gel tray–allowsseparations to be performed fasterand at higher voltage, without lossof resolution
Cooling ports–connect the cooledbase to an optional chiller forenhanced cooling (see page 139).
Accessories and Replacement PartsCat. # DescriptionSUB20-TANK Buffer Chamber AssemblySUB20C-TANK Buffer Chamber Assembly w/Cooling PortsSUB20-LID Lid for SUB20 and SUB20CSUB20-UT 20 x 20 cm Running Tray w/Casting GatesSUB20-CG Casting Gates w/Silicone Gasket–2 pcsSUB20-CS Colored Loading Strips–6 pcsSUB20-GS Gel Scoop for SUB20SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcs
SUB20 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB20-C1-16 16 1 8.5 35SUB20-C1.5-16 16 1.5 8.5 55SUB20-C2-16 16 2 8.5 75SUB20-C1-20MC 20* 1 7 30SUB20-C1.5-20MC 20* 1.5 7 45SUB20-C2-20MC 20* 2 7 60SUB20-C1-28 28 1 5 20SUB20-C1.5-28 28 1.5 5 30SUB20-C2-28 28 2 5 40SUB20-C1-40MC 40* 1 3 13SUB20-C1.5-40MC 40* 1.5 3 19SUB20-C2-40MC 40* 2 3 25
*Microtiter spacing for use with a multichannel pipette
SUB20 Casting GatesThe included casting gates convenientlyand efficiently seal the running traywithout the hassles of using tape.
SUB20 Maxi-Standard andSUB20C Maxi-Cooled SubmarineAccessories and Replacement Parts
SUB20-UT
SUB20-LID
SUB20C-TANK
SUB20-TANK
Toll Free: (800) 227-4750 • Tel: +1 (508) 893-8999E-mail: sales@hoeferinc.com •Web: www.hoeferinc.com
NUCLEIC
ACID
ELECTROPHORESIS
48
SUB25 Maxi-Plus Standard andSUB25C Maxi-Plus Cooled Submarine
Gel Electrophoresis UnitsIdeal for high throughput screening ofnucleic acids, particularly PCRproducts and in genotyping studies
Features and BenefitsLarge format–25 x 30 cm gel tray isideal for high throughput techniques
Multichannel pipette compatiblecombs–with a maximum 52 samplethroughput–reduce gel loading time
Color-coded and height adjustablecombs–easily identify combthickness at a glance and controlwell depth
Buffer recirculation ports–may beconnected to a peristaltic pump(see page 141) for buffer recircu-lation during electrophoresis tomaintain buffer pH and preventionic gradient formation
Colored loading strips–for easy welldetection when loading
UV-transparent running tray–allowsthe user to image the gel withoutrisk of damage due to handling
Side handles–for safe and easy trans-portation around the laboratory
Ordering InformationCat. # DescriptionSUB25 Maxi-Plus Standard Submarine Gel Unit
Technical SpecificationsGel Dimensions (w x l) . . . . . . . . . . . . . . . 25 x 30 cmMaximum Buffer Volume . . . . . . . . . . . . . 3 LMaximum Power Settings . . . . . . . . . . . . . 1000 V, 500 mA, 500 WMaximum Sample Capacity . . . . . . . . . . . 624Maximum Running Temperature . . . . . . . . 45°CIndoor Use . . . . . . . . . . . . . . . . . . . . . . . . 4-40°CHumidity . . . . . . . . . . . . . . . . . . . . . . . . . Up to 80%Unit Dimensions (w x l x h) . . . . . . . . . . . 35 x 56 x 9 cmSafety Certifications . . . . . . . . . . . . . . . . . EN61010-1, CE
Includes:• Buffer Chamber Assembly • 1 mm thick, 26 well combs–6 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates
The SUB25 Maxi-Standard Submarine Electrophoresis Unit provides a25 x 30 cm gel-running tray with 12 comb positions accommodatinga maximum throughput of 624 samples. The SUB25C Maxi-CooledSubmarine Electrophoresis Unit has the same efficient design asthe SUB25 with the addition of a cooling base that can beconnected to a recirculation bath allowing high voltageelectrophoretic separations without overheating.
Cat. # DescriptionSUB25C Maxi-Plus Standard Submarine Gel Unit w/Cooling Base
Includes:• Buffer Chamber Assembly w/Cooling Ports • 1 mm thick, 26 well combs–6 pcs• Safety Lid • Colored Loading Strips• High Voltage Leads • Buffer Recirculation Ports–2 pcs• Running Tray w/Casting Gates
SUB25
SUB25C
Toll Free: (800) 227-4750 • Tel: +1 (508) 893-8999E-mail: sales@hoeferinc.com •Web: www.hoeferinc.com
NUCLEIC
ACID
ELECTROPHORESIS
49
Additional Features andBenefits of the SUB25CCooled base–covering the entire25 x 30 cm gel tray–allowsseparations to be performed fasterand at higher voltage, without lossof resolution
Cooling ports–connect the cooledbase to an optional chiller forenhanced cooling (see page 139).
Accessories and Replacement PartsCat. # DescriptionSUB25-TANK Buffer Chamber AssemblySUB25C-TANK Buffer Chamber Assembly w/Cooling PortsSUB25-LID Lid SUB25 and SUB25CSUB25-UT 25 x 30 cm Running Tray w/Casting GatesSUB25-CG Casting Gates w/Silicone Gasket–2 pcsSUB25-CS Colored Loading Strips–6 pcsSUB25-GS Gel Scoop for SUB25SUB-LEAD Replacement High Voltage LeadsSUB-SG Silicone Gasket, 1 meterSUB-BRP Buffer Recirculation Ports–2 pcs
SUB25 CombsCat. # # of Wells Thickness Well Width Volume in 5 mm
(mm) (mm) DeepWell (µl)SUB25-C1-26MC 26* 1 7 30SUB25-C1.5-26MC 26* 1.5 7 45SUB25-C2-26MC 26* 2 7 60SUB25-C1-52MC 52* 1 3 13SUB25-C1.5-52MC 52* 1.5 3 20SUB25-C2-52MC 52* 2 3 25
*Microtiter spacing for use with multichannel pipette
SUB25 Casting GatesThe included casting gates convenientlyand efficiently seal the running traywithout the hassles of using tape.
SUB25 Maxi-Plus Standardand SUB25C Maxi-Plus CooledSubmarine Accessories andReplacement Parts
SUB25-LID
SUB25-UT
SUB25-TANK
SUB25C-TANK