Ananlisis Fragmen DNA

Post on 03-Dec-2015

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description

Analisis fragmen DNA

Transcript of Ananlisis Fragmen DNA

Diketahui:

Non-Template Strain :

5’ CAAAACGGTTGACAACATGAAGTAAACACGGTATAATGTAGGAATTAAGGAGGTATATGATAACTAATTTTGACCACCA

TTGCTATGCCTGGATGATACTAGGGCAATCAGTCCGCTATCCATAGCCCCAAAGTTAAAAA 3’

Berdasarkan Non-Template Strain, diperoleh bagian promotor (consensus sequence):

Up-element N -35 region Spacer -10 region Spacer RNA start

5’ CAAAACG G TTGACA ACATGAAGTAAACACGG TATAAT GTAGGA ATT AAGGAGGTATATGATA ACTAAT TTT GAC CAC CAT

TGC TAT GCC TGG ATG ATA CTA GGG CAA TCA GTC CGC TAT CCA TAG CCCCAAAGTTAAAAA 3’

Diperoleh mRNA (hasil transkripsi):

5’ AUU AAGGAGG UAU AUG AUA ACU AAU UUU GAC CAC CAU UGC UAU GCC UGG AUG AUA CUA GGG CAA UCA GUC CGC

UAU CCA UAG CCCCAAAGUUAAAAA 3’

Shine-

Dalgarno

Sequence Start

Kodon

Stop

Kodon

Nama : Dea Santika Rahayu

NIM :1200387

Kelas : B

Hasil Translasi:

5’ AUU AAGGAGG UAU AUG AUA ACU AAU UUU GAC CAC CAU UGC UAU GCC UGG AUG AUA CUA GGG CAA UCA GUC CGC Met- ileu- thr- asn- phe- asp- his- his- cys- tyr- ala- trp- met- ileu- leu- gly- gin- ser- val- arg-

UAU CCA UAG CCCCAAAGUUAAAAA 3’ tyr- pro-

Shine-

Dalgarno

Sequence Start

Kodon

Stop

Kodon