Using of MacMaster technique for the identification of

helminth fauna of carnivores of the Białowieża Primeval Forest

Yakovlev, Y.Kołodziej-Sobocińska, M.

I. I. Schmalhausen Institute of Zoology NAS of UkraineDepartment of Parasitology

Mammal Research Institute PAS

XV International Conference of the Ukrainian Scientific Society of Parasitologists15-18 October 2013 Chernivtsi, Ukraine

Sampling

13

22

12

5 5 5

1

16

7

3

1

CL VV MP MV MM MeMe

Feb.'12 Oct.'12 Feb.'13

Dynamics of fecal sampling

High conservation

level zone

“BNP” wolf pack

Recreation zone

“Leśna 1” wolf pack

Białowieża National Park, Białowieża, Poland

Mammal Research InstitutePAS

Methods

Making of suspension with the supersaturated sodium chloride

solution

Removing of thehard pieces

Filling of the MacMaster camera,

microscopy and identification of eggs

4 grams of feces

Quantifying

Mathematics methods

Counting of [eggs, larvae, cysts]/gram of feces

Counting of Prevalence

Counting of Abundance

Hosts species

Quantity of samples

Parasite species P, %

Canislupus

51 Alaria alata (ALA), Ancylostoma caninum (ANC), Coccidiasina (COC), Echinococcus sp. (ECH), Isosporasp. (ISO), Spirocerca lupi (SPL),Taenia pisiformis, Taenia sp. (TAE), Toxocara canis (TOC), Toxascaris leonina (TOL), T. mystax (TOM), Trichuris vulpis (TVU), Uncinaria stenocephala (UNS), unidentified filaroid (1 case, 22 l/g) and rhabditid (1 case, 81 l/g) larvae

80

Vulpesvulpes

19 A. alata, Coccidiasina, Echinococcus sp. , Isospora sp., Mesocestoides lineatus, S. lupi, T. canis, T. mystax, T. leonina, T. vulpis, U. stenocephala

84

Mustela putorius

8 Coccidiasina, T. vulpis, unidentified rhabditid larvae (1 case, 1176 l/g)

63

Mustela vison

6 A. alata, Euparyphium meles (EUM), S. lupi, T. canis, T. vulpis

83

Martesmartes

5 Isospora sp., Molineus patens (MOP), T. vulpis, U. stenocephala

100

Meles meles

1 T. vulpis (80 e/g) -

Canis lupus L., 1758Leśna 1, n=9

Prevalence

56

11

0

0

0

11

0

11

0

11

56

22

100

020406080100

43

10

5

2

7

0

10

14

10

14

55

17

76

0 20 40 60 80 100

Leśna 1, n=9 BNP, n=42

BNP, n=42296

8

0

0

0

8

0

147

0

9

428

21

0100200300400500

176

13

2

1

20

0

121

14

330

154

475

18

0 100 200 300 400 500

Abundance

Proglottids of Taenia pisiformis (1 case)

Ph

oto

by

M. S

hk

vy

rya

0

10

20

30

40

50

60

70

80

90

100

ALA ANC COC ECG ISO SPL TAE TOC TOL TOM TVU UNS Total

Feb' 12 Oct' 12 Feb' 13

Dynamics of Prevalence (Canis lupus)BNP wolf pack (n=42)

Vulpes vulpes L., 1758

42

5

5

26

16

5

5

5

42

42

020406080100

601

12

5

652

22

186

3

20

213

64

0 100 200 300 400 500 600 700

n=19

Proglottids of Mesocestoides lineatus (1 case)

Martes martes L., 1758

n=5

60

20

80

20

050100

505

3

45

24

0 200 400

Mustela putorius L., 1758

n=8

13

50

050100

841

100

0 500

50

17

17

17

17

17

050100

266

145

29

28

12

0 100 200 300

Mustela vison Schreber, 1777n=6

Ph

oto

by

M. S

hk

vy

rya

Conclusion1. According to the helminthovoscopy, helminth

fauna of wild carnivores of Białowieża NationalPark presented by four species of cestodes, ninespecies of nematodes and two species oftrematodes.

2. Identified that all studied species of hosts infectedby T. vulpis, and five from seven species of hostsare infected by U. stenocephala.

3. Was found two cases of infection byEchinococcus sp. – in wolf (24 e/g) and in fox(97 e/g).

Thank You for attention!

The research was supported by the European Commission's project No. PIRSES-GA-2009-247652 (BIOGEAST)