Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and...

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Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and UV-B using Proteomic and Biochemical Approaches * Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras Hindu University, Varanasi-221005, INDIA *Corresponding author: [email protected] Abstract Anabaena Introduction Anabaena Synechocystis Anabaena

Transcript of Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and...

Page 1: Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and UV-B using Proteomic and Biochemical Approaches

Understanding Interactive Protein Network in the

Cyanobacterium Anabaena PCC 7120 under Salt and UV-B

using Proteomic and Biochemical Approaches

*

Molecular Biology Section, Laboratory of Algal Biology, Center of Advanced Study in Botany, Banaras

Hindu University, Varanasi-221005, INDIA

*Corresponding author: [email protected]

Abstract

Anabaena

Introduction

Anabaena

Synechocystis

Anabaena

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Anabaena Anabaena

Anabaena

Materials and methods

Organism and growth conditions

Anabaena

Experimental setup and treatment

Anabaena

Growth measurement

Pigment estimation

Anabaena

Measurement of ATP and NADPH/NADH content

Measurement of lipid peroxidation (TBARS content), total peroxide, proline, thiol and ammonium contents

Enzymatic assays

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Total protein extraction and 2-DE separation

Anabaena

Image analysis

Results

Growth measurement and pigment estimation

Anabaena

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Total peroxide, thiobarbituric acid, proline and thiol content

Anabaena

NADPH and ATP content

Anabaena

Glycolate oxidase assay and ammonium content

Anabaena

Enzymatic assays (SOD, CAT, APX, GR)

Protein expression profile in response to salt and UV-B stresses

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Anabaena .

Spot

No.

Protein name

(showing homology with)

Mr (kDa)/

pI Theor.

Mr (kDa)/

pI Exper.

Accession

no.

No. of

peptides

matched

Mowse

score

Energy metabolism (photosynthesis, photorespiration and carbon fixation)

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Oxidative stress defence proteins

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Protein synthesis and folding

Nostoc

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Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nitrogen metabolism

Nostoc

Nostoc

Nostoc

Nostoc

Cell signalling

Nostoc

Fatty acid biosynthesis

Nostoc

Hypothetical proteins

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Nostoc

Spot

no.

Gene name BlastP result %

homol

ogy

Page 9: Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and UV-B using Proteomic and Biochemical Approaches
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Discussion

Anabaena

Anabaena

Anabaena

Anabaena

Spirulina

Anabaena doliolum

Anabaena

Anabaena doliolum

Anabaena variabilis

Anabaena variabilis

Anabaena

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Anabaena

Campylobactor jejuni

Anabaena doliolum

Synechocystis

Conclusion

Acknowledgements

Page 12: Understanding Interactive Protein Network in the Cyanobacterium Anabaena PCC 7120 under Salt and UV-B using Proteomic and Biochemical Approaches

References

Anabaena

Anabaena doliolum

Anabaena doliolum

Glycine max

Trichodesmium

Microcoleus vaginatus

in

vitro

Nostoc commune

Synechocystis

Synechosystis

.

Synechocystis

Synechocystis

Spirulina platensis

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Spirulina

platensis

Campylobacter jejuni

Campylobacter jejuni

Synechocystis

Anabaena doliolum

Scenedesmus bijugatus

Anabaena all3940

E. coli

Anabaena

Anabaena

Nostoc muscorum

Populas gebrica 57

Glycine max

Anabaena doliolum

Spirulina platensis

Scytonema javanicum

Brucella melitensis

Anabaena variabilis


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