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Understand the structuring of wheat-legume cakes topromote product innovation and to design new

formulation tools for the industryAnne-Flore Monnet, Marie-Helene Jeuffroy, Camille Michon

To cite this version:Anne-Flore Monnet, Marie-Helene Jeuffroy, Camille Michon. Understand the structuring of wheat-legume cakes to promote product innovation and to design new formulation tools for the industry.Second International Legume Society Conference, Oct 2016, Tróia, Portugal. �hal-01637755�

SecondInternationalLegumeSocietyConference

Legumesforasustainableworld

BookofAbstracts

11th–14thOctober,

2016

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Contents PagesWelcomeAddresses

FromthePresidentofILS 3FromtheLocalConveeners 4

Organizers 5Conveners 6ScientificCommittee 7LocalOrganizerCommittee 8Sponsors 9Program 11KeyLecturers 27SessionAbstracts 49

Session1 50Session2 56Session3 72Session4 87Session5 116Session6 121Session7 126Session8 155Session9 178Session10 185Session11 192Session12 202Session13 219Session14 247Session15 263Session16 270Session17 275Session18 282

ListofParticipants 335

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WelcomeAddressesFromthePresidentofILS(2011-2016) The LegumeSocietywasfoundedin2011afterclosureofformerEuropean Association for Grain Legume Research (AEP). Ourmissionwas becoming the society of reference for ALL legumesWORLWIDE,thisis,extendingtherangeofcropscoveredandtheareaofreference.Afterfivedifficultyearswithabsolutelylackofsponsorsandanykind of financial support, we can proudly realize that LegumeSocietyhasconsolidatedwithgreatsuccessandhasapromisingfuture.Thiswaspossibleonly thanks to thevoluntaryworkofanumberofcommittedpeople.We have been able to establish a solid and reliable series oftriennial legume-devotedconferencesaswellasadisseminationmagazine (Legume Perspectives), with a real niche serving as adisseminationtoolforthelegumecommunity.First Legume Society Conference was successfully organized atNovi Sad, Serbia in 2013. Second is taking place now (2016) atTroia,Portugal,togetherwithanumberofsatelliteevents.Thirdis already planned at Poznan, Poland in 2019.We are sure thatthisreliableseriesofmultidisciplinaryconferenceswillbeofgreatinterest to legume experts, extending it to stake holders. Weshould acknowledge the great efforts made by the localorganizersoftheseevents.At the General Assembly a new Scientific Committee will beelected thatwill for surebring the renewedenergiesneeded tocontinue with Legume Society endeavor. We all shall offer oursupportandgratitudeandwishthemsuccessforfuture.DiegoRubiales

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FromtheLocalConveenersWe cordially welcome you to the Second International LegumeSocietyConferenceintheInternationalYearofPulses.Inaworldurgentlyrequiringamoresustainableagriculture,foodsecurity andhealthierdiets, thedemand for legumecrops is ontherise.Thisgrowthisfosteredbythe increasingneedforplantprotein and for more sustainable and environmentally friendlysoundagriculturalpractices.Food, feed, fiber andeven fuel areall products that come fromlegumes–plantsthatgrowwithlownitrogeninputsandinharshenvironmentalconditions.The second ILS Conference is welcoming more than 370attendees from 53 countries, showing the internationalrecognition of the work developed by ILS and the globalimportanceofresearchinlegumes.The general aimof the conference is to update and discuss theknowledge developed in legume research, in its various facets,from their genomes sequencing, their genetic resources, theirenvironmental response and adaptation, to their use inagriculturalsystems.WewishyouafruitfulandrewardingconferenceandapleasantstayinwonderfulpeninsulaofTróia.PedroFevereiro,CarlotaVazPattoandSusanaAraújo

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OrganizersInternationalLegumeSocietyThe International Legume Society wasfounded in 2011with two primarymissions.Oneofthemwastotreasuretherichlegumeresearch tradition of the former EuropeanAssociationforGrainLegumeResearch(AEP),withemphasisoncarryingoutitsthetrienniallegume-devotedconferences.Anotheroneistofulfillalong-termstrategyoflinkingtogethertheresearchonalllegumesworldwide, from grain and forage legumes pharmaceuticaland ornamental ones and from the Old World to theAmericas.

ITQBNOVAThe Instituto de Tecnologia Química eBiológica António Xavier (ITQB NOVA)is a research and advanced traininginstitute of Universidade Nova deLisboa. Themissionof ITQBNOVA is to carry out scientificresearch and postgraduate teaching in life sciences,chemistry and associated technologies, for the benefit ofhuman health and the environment. ITQB’s highlymultidisciplinary nature makes it a leading centre foradvancedtrainingofresearchersinPortugal.

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Conveners

PedroFevereiro–ITQBNOVA

CarlotaVazPatto–ITQBNOVA

SusanaAraújo–ITQBNOVA

DiegoRubiales-ScientificCoordinator–IAS-CSIC

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ScientificCommittee(inalphabeticorder)

MichaelAbberton-IITA,NigeriaShivKumarAgrawal-ICARDA,SyriaPaoloAnnicchiarico-CREA-FLC,ItalyStephenE.Beebe-CIAT,ColombiaCharlesBrummer–Univ.California,USAAdrianCharlton-FERA,UKGerardDuc-INRA,FranceNoelEllis–NewZealandPedroFevereiro–ITQBNOVA,PortugalJudithLichtenzveig-CurtinUniv.,AustraliaKevinMcPhee-NorthDakotaStateUniv.,USAAleksandarMikić-IFVC,SerbiaEduardoRosa-UTAD,PortugalDiegoRubiales-CSIC,SpainFredStoddard–Univ.Helsinki,FinlandRichardThompson-INRA,FranceTomWarkentin–Univ.Saskatchewan,Canada

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LocalOrganizerCommittee(inalphabeticorder)NunoAlmeida-ITQBNOVAAnaBarradas–FertipradoRitaCaré–CiBManuelaCosta–Univ.MinhoIsabelDuarte–INIAVSofiaDuque–ITQBNOVAPedroFevereiro–ITQBNOVASusanaLeitão-ITQBNOVAJoséRicardoParreira-ITQB-NOVAEduardoRosa–UTADMartaVasconcellos–ESB-UCPCarlotaVazPatto-ITQBNOVAManuelaVeloso–INIAV

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SponsorsTheorganizersacknowledgethesupportofthefollowingSponsors:

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Program

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October 10th-11th 2016: Satellite events (registrationneeded)10th-11th October Ascochyta 2016 Workshop - RoomArrábidaIV(programinhttp://www.ascochyta2016.aweb.net.au/)11th October 09:00-18:00h LEGATO project meeting(partnersonly)-RoomsArrábidaIII&Douro11th October 09:00-18:00h EUROLEGUME project meeting(partnersonly)-RoomGuadiana11th October 09:00-18:00h ABSTRESS project meeting(partnersonly)-RoomAtlântico.October 11th 2016: 18:00-20:00 ILS2 ConferenceRegistrationOctober12th201608:00-12:00Registration(cont.)09:00-09:15Welcomeaddress-RoomArrábidaI&II09:15-10:30 Session 1, plenary: Legumes value chain:market requirements and economic impact - RoomArrábidaI&IIChaired by Eduardo Rosa (UTAD, Portugal) and AdrianCharlton(FERA,UK)09:15-09:45 Key lecture - Hakan Bahceci: Forging a NewFuture for Pulses: Addressing research challenges with themomentumoftheUNInternationalYearofPulses09:45-10:00 P. Iannetta: Main-streaming pulses: exploring localsolutionstosupplychainlimitations10:00-10:15F.Muel:Wouldtheproteinfractionbethefutureofoilandgrainlegumecropsby2030?

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10:15-10:30 G. Dubois: Legume future from European Unionperspective:Horizon2020,EIP-AGRIandCAP10:30-11:00Coffeebreak11:00-12:00Session2,plenary:Legumesandenvironment-RoomArrábidaI&IIChaired by Richard Thompson (INRA, France) and DiegoRubiales(CSIC,Spain)11:00-11:30Keylecture-Marie-HélèneJeuffroy:Validatingthe environmental benefits of legumes requires a territorialapproach11:30-11:45 B. Cupina: Environmental impact of introducing legumesintocroppingsystem11:45-12:00C.Watson:Sustainablemanagementof grass-white cloverleysinley-arablefarmingsystems

12:00-13:00Posterviewing13:00-14:30Lunch14:30-16:00Parallelsessions-RoomArrábidaIIISession3,parallel:Beneficial legume-microbeinteractionsAChaired by Carmen Bianco (Univ. Bari, Italy) and PedroFevereiro(ITQBNOVA,Portugal)14:30-15:00Keylecture-JensStougaard:Receptormediatedsignalinginlegumesymbiosis15:00-15:10 J. Keller: Symbiotic genes expression in a context ofnitrogen-fixingsymbioticspecificityinLupinus15:10-15:20M.Vosatka:Beneficialmicrobesassociatedwithlegumes15:20-15:30 V. Bourion: Partner choice in a core collection of peainoculatedbyamixoffiveRhizobiumstrains

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15:30-15:40 M. Lepetit: Improving adaptation of legume-rhizobiumsymbiosistothesoilenvironment

15:40-16:00 General discussion on beneficial legume-microbeinteractionsSession4,parallel:Geneticresources-RoomArrábidaIVChaired by Hari Upadhyaya (ICRISAT) and Rodomiro Ortiz(SLU,Sweden)14:30-15:00Keylecture-NoelEllis:Whereareweafter150yearsoflegumegenetics?15:00-15:10 E. vonWettberg: Using collections of wild relatives ofchickpeatounderstanddomestication15:10-15:20M.Carvalho:Characterizingthegeneticdiversityofcowpeaaccessionsusingahigh-densitySNP15:20-15:30 K. Fischer: LupiBreed - Valorisation of novel geneticvariabilityinnarrow-leafedlupin15:30-15:40M.Nelson:Domesticbliss?Causesandconsequencesofamoderneradomesticationevent15:40-16:00Generaldiscussionongeneticresources16:00-16:30CoffeebreakandPosterviewing16:30-17:30ParallelsessionsSession 5, parallel: Legumes value chain: marketrequirements and economic impact (cont.) - RoomArrábidaIIIChaired by FrédéricMuel (Terres Inovia, France) and PeteIannetta(JHI,UL)16:30-16:40L.Bedoussac:Evaluatingcereal-legumeintercropstowardssolecrops16:40-16:50 M. Magrini: Escaping from grain-legume socio-technicalsystemlock-in16:50-17:00A.Bentaibi:Analysisofsocialandorganizationalaspectsoffoodlegumeschain

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17:00-17:10 D. Lemken: The re-innovation of Mixed Cropping - whocares?-Trialwillingness17:10-17:30GeneraldiscussiononlegumesvaluechainSession6,parallel:Rootdiseases-RoomArrábidaIVChaired by Julie Pasche (North Dakota St. Univ., USA) andNicolasRispail(CSIC,Spain)16:30-16:40L.Gentzbittel:Quantitative response ofM. truncatula toverticilliumwilt.16:40-16:50 M.L. Pilet-Nayel: Genetics of pea resistance toAphanomyceseuteichesinthegenomicsEra16:50-17:00C.Coyne:Progress on understanding genetic resistance toFusariumrootrotinpea17:00-17:10 S. Chatterton: Molecular quantification of pathogenicFusariumspp.insoiltopredictpearootrotrisk17:10-17:30Generaldiscussiononrootdiseases17:30-18:30Postersession1:Slotsofflashpresentations(3min+2minquestions)fromselectedposters(topicsoftheday)-RoomArrábidaI&IIChairedbyAlessioCimmino(Univ.Naples, Italy)andGeorgCarlsson(SLU,Sweden)A.SeabraPinto:Doconsumers'valuethenewuseoflegumes?

C. Ghoulam: Intercropping legume-cereals is a system to value legume-rhizobiasymbiosisJ. Fustec: Synergy between crop diversity and earthworm communityimprovecropyieldsC.Lotti:AnovelsourceofgeneticdiversityincultivatedchickpeaasrevealedbyGBSandgenotypingM.G. Tobar-Pinon: Genetic diversity of the Gautemalan climbing beancollectionM. Ruland: Temporal and regional development of lentil populations bynaturalselectionon-farmA. Moussart: Effect of pea sowing date on aphanomyces root rotdevelopmentandyieldlosses

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A.Lesné:ConstructionandevaluationofNear-IsogenicLinesforresistancetoAphanomyceseuteichesinpeaR.Tollenaere:NestedAssociationMappingforresistancetoAphanomycesinM.truncatulaC.Bianco:Theauxinindole-3-aceticacid(IAA)ismorethanaplanthormone20:45ThirdInternationalLegumeFootballCupOctober13th20168:30-10:00Session7,plenary: Legumes in foodand feedandotheralternativeuses-RoomArrábidaI&IIChairedbyMaria CarlotaVaz Patto (ITQBNOVA, Portugal)andAmbujB.Jha(Univ.Saskatchewan,Canada)08:30-09:00 Key lecture - Frédéric Marsolais: Using beanswithnovelproteincompositionsfornutritionalimprovement09:00-09:15M.Bronze:Thehiddenphenoliccontentoffababeans09:15-09:30 J.C. Jimenez-Lopez: Use of narrow-leafed lupin b-conglutinproteinsinhumanfoodtotacklediabetes09:30-09:45 C. Domoney: Genetic diversity in pea and its impact onstrategiesforseedqualityimprovement09:45-10:00 A.F. Monnet: Understanding the structuring of wheat-legumecakestopromoteproductinnovation10:00-10:30CoffeebreakandPosterviewing10:30-12:00 Session 8, plenary: Frontiers in legumegeneticsandgenomics-RoomArrábidaI&IIChairedbyRobertoPapa(Univ.LeMarche,Italy)andMartaSantalla(CSIC,Spain)10:30-11:00 Key lecture - Judith Burstin: Towards thegenomesequenceofpea:atributetoMendel11:00-11:15 P.Wan: Genome sequencing of Vigna angularis providesinsightintohighstarchandlowfat

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11:15-11:30 P. Annicchiarico: Genotyping-by-sequencing and itsexploitationinforageandgrainlegumebreeding11:30-11:45S.Kaur:Applicationof historical data fromAustralian lentilbreedingprogramingenomicselection11:45-12:00G.Boutet:WGGBS in pea without reference genome anddataassembly12:00-13:00 Poster session 2: Slots of 3 min flashpresentations (+ 2 min questions) from selected posters(topicsoftheday)--RoomArrábidaI&IIChaired byNunoAlmeida (ITQBNOVA, Portugal) and SaraFondevilla(CSIC,Spain)E.Collado:Peastraw:anadvantageousco-productindairygoatdietsC. Arribas-Martinez: Nutritional and nutraceutical characterization ofextrudedgluten-freesnacksM.Książkiewicz:Genesinvolvedinfloweringtimeregulationinwhitelupin

R.V. Penmetsa:Mendel's enduring legacy: orthologs of two of his sevenfactorsinmultiplecurrentdaycroplegumesR.Papa:BeanAdapt:ThegenomicsofadaptationduringcropexpansionincommonbeanA.Sarkar:TheLathyrussativusgenomeprojectH. Bobille: Effect of soil water deficit on amino acid exudation in PisumsativumrootsC. Le May: Plant disease complex: antagonism and synergism betweenpathogensA. Cimmino: Necrotrophic effectors produced by fungal pathogens oflegumecropsA.Quillévéré-Hamard:Genetic andphenotypicdiversityof pea isolatesofAphanomyceseuteichesinFranceL. Aguirrezabal: Modelling the effect of assimilates availability on seedweightandcompositioninsoybean13:00-14:30Lunch

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14:30-16:00ParallelsessionsSession 9, parallel: Legumes in food and feed and otheralternativeuses(cont.)-RoomArrábidaIIIChaired by Ruta Galoburda (Latvia Univ. of Agriculture,Latvia)andTomWarkentin(Univ.Saskatchewan,Canada)14:30-14:40 E. Tormo: A meta-analysis to assess the effect of finegrinding,dehullingandpelleting14:40-14:50E.Mecha:ProteinqualityofdifferentPortuguese varietiesofcommonbean14:50-15:00 L. Proskina: Economic factors of using the legumes inbroilerchickensfeeding15:00-15:10M.C.Serrano: Tocopherols and carotenoids diversity in achickpeagermplasm15:10-15:20 A. Clemente: Bowman-Birk inhibitors from legumes andmammalianguthealth15:20-15:30A.B.Jha:EvaluationofapeagenomewideassociationstudypanelforfolateprofilesbyUPLC-MS/MS15:30-16:00GeneraldiscussiononlegumesinfoodandfeedandotherusesSession 10, parallel: Frontiers in legume genetics andgenomics(cont.)-RoomArrábidaIVChaired by Bernadette Julier (INRA, France) and KevinMcPhee(NorthDakotaStateUniv.USA)14:30-14:40 C. Le Signor: A protein quantity loci approach combinedwithagenome-wideassociationstudy14:40-14:50 M.C. Vaz Patto: Using genomics to decipher the grainlegumesqualityriddle

14:50-15:00A.M.Torres:Strategiesandadvancestoidentifycandidategenesforlowvicine-convicineinfababean15:00-15:10M.Santalla:HomologuesofArabidopsisgenes involved inphotoperiodresponseincommonbean15:10-15:20P.Smykal:Wild peaP. fulvum andP. elatius chromosomesegmentsubstitutionlinesincultivatedpea

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15:20-15:30A.Campa:Delimitingthephysicalpositionsofanthracnoseresistanceclusters15:30-16:00Generaldiscussiononfrontiersingeneticsandgenomics16:00-16:30CoffeebreakandPosterviewing16:30-17:45ParallelsessionsSession11,parallel:Frontiersinplantandcropphysiology-RoomArrábidaIIIChaired by Christophe Salon (INRA, France) and LuisAguirrezabal(CONICET,Argentina)16:30-17:00Keylecture-PhilMullineaux:The identificationofnovelgenescontrollingplant-environmentinteractions17:00-17:10 J. Vorster: Drought-induced transcriptome changes insoybeancrownnodules

17:10-17:20 R. Metzner: In vivo monitoring of the development oflegumeroots,nodulesandpods17:20-17:30G.Louarn:Acommonshootdevelopmental frameworkforperenniallegumespecies

17:30-17:45 General discussion on frontiers in plant andcropphysiologySession 12, parallel: Integrated pest and diseasemanagement-RoomArrábidaIVChaired by Jenny Davidson (PIRSA-SARDI, Australia) andChristopheLeMay(INRA,France)16:30-17:00Keylecture-SeidKemal:Integrateddiseaseandinsectpestmanagementpestandincool-seasonfoodlegumes17:00-17:10 A. Baranger: PISOM: Ideotypes, Systems, Surveys of peaandfababeanMaindiseases17:10-17:20Y.Mehmood: The AustralianAscochyta rabiei populationstructure

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17:20-17:30 W. Chen: Chickpea damping-off caused by metalaxylresistantPythiumintheUSPacificNorthwest17:30-17:45 General discussion on integrated pest anddiseasemanagement17:55-19:00ILSGeneralAssembly-RoomArrábidaI&II20:45ThirdInternationalLegumeFootballCupOctober14th20168:30-10:00 Session 13, plenary: Frontiers in legumebreeding-RoomArrábidaI&IIChaired by Wolfgang Link (Georg-August-University,Germany)andGerardDuc(INRA,France)08:30-09:00 Key lecture - Scott Jackson: Contribution ofepigeneticvariationtoimprovement09:00-09:15 B. Julier: QTL detection for forage biomass of alfalfa inmixturewithaforagegrass09:15-09:30A.Charlton: Improving the resistance of legume crops tocombinedabioticandbioticstress09:30-09:45 M. Pazos: Integrated platform for rapid genetic gain intemperategrainlegumesandwildCicerspecies09:45-10:00A.Sarker:Broadeningthegeneticbaseoflentil10:00-10:30CoffeebreakandPosterviewing10:30-12:00 Session 14, plenary: Frontiers in legumeagronomy-RoomArrábidaI&IIChairedbyErikS.Jensen(SLU,Sweden)andSusanaAraújo(ITQBNOVA,Portugal)

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10:30-11:00Keylecture-EricJustes:Synthesisontheeffectsof grain legume insertion and cereal-grain legume intercrops inlowinputcroppingsystemsinSouthernFrance11:00-11:15E.Pelzer:Designandassessmentoflegume-basedcroppingsystemswithstakeholdersinEurope11:15-11:30 C. Porqueddu: Performance of legume-based annualforagecropsinthreeMediterraneanregions11:30-11:45 A. Lingner: Legume-based mixed cropping systems mayhavehigherwateruseefficiency11:45-12:00 G. Carlsson: Participatory development of grain legume-cerealintercropsforenhancedproductivity12:00-13:00 Poster session 3: Slots of 3 min flashpresentations (+ 2 min questions) from selected posters(topicsoftheday)--RoomArrábidaI&IIChairedbySofiaDuque(ITQBNOVA,Portugal)andAngelM.Villegas-Fernández(CSIC,Spain)L.Wiesel:Starterfertilizers:Dotheyinfluencerhizobialpopulationsinviningpeafields?R.Bowness:Evaluationof agronomicpracticesonproductionof ClearfieldredlentilinAlberta,CanadaR.Seljåsen:Nitrogenavailability frompeasand fababeansaspre-crops tobroccolifollowedbylettuceJ. Rebola Lichtenberg: Biomass production in mixed short-rotationwoodycroppingofPopulushybridsandRobiniaM. Ćeran: Discovering genetic signatures of selection in the elite soybeangermplasmJ.Aper:Flowerabscissionratesofearly-maturingsoybeanvarietiesC.Holdt:GeneticstudiesofwinterhardinessinpeaA.Scegura:Marker-assistedbackcrossselectionofvirusresistanceinpeaJ.J.Ferreira:GeneticresistancetopowderymildewincommonbeanM. Pérez-de-la-Vega: RNA-seq analysis of gene expression in lentils inresponsetoAscochytalentisinfectionV. Vernoud: A transcriptomic approach identifies candidate genes fordroughttoleranceinpea

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13:00-14:30Lunch14:30-16:00ParallelsessionsSession15,parallel:Frontiers in legumebreeding(cont.)-RoomArrábidaIIIChairedbyPaoloAnnicchiarico(CREA,Italy)14:30-14:40B.Taran:Genomicpredictionforseedsizeinchickpea

14:40-14:50 L. Brünjes: Faba bean lines differ in their contribution aspollendonortocross-fertilizedseed14:50-15:00 L. Pecetti: Assessing and overcoming genetic trade-offs inbreedinggrazing-tolerantalfalfa15:00-15:10T.Warkentin:Enhancingthenutritionalqualityoffieldpea15:10-15:20 B. Rewald: Machine-learning approaches for root traitdeterminationanddifferentiationofcultivars15:20-15:30R.Madhavan-Nair:AnInternationalnetworkto improvemungbeanbreedingandproduction15:30-16:00 General discussion on frontiers in legumebreedingSession16,parallel:Advancesinlegumeagronomy(cont.)-RoomArrábidaIVChaired by Fred Stoddard (Univ. Helsinki, Finland) andClaudioPorqueddu(CNR,Italy)14:30-14:40G.Corre-Hellou:Ecosystemservicesprovidedbylegumesandexploitedbystakeholders14:40-14:50 M. Guinet: Quantification of nitrogen fluxes andexplanatoryplanttraits14:50-15:00 N. Carton: Cereals as companion crops in cereal-grainlegumeintercrops:caseoflupin15:00-15:10E.Journet:Intercroppinglentilwithspringwheatinorganicfarming

15:10-15:20S.Guy:DiversificationofUSAdrylandcroppingsystemsusingautumn-sownwinterpea15:20-15:30 J. Streit: Quantitative analysis of the root distribution in afababean-wheatintercroppingsystem

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15:30-16:00 General discussion on advances in legumeagronomy16:00-16:30CoffeebreakandPosterviewingSession 17, parallel: Legumes and environment (cont.) -RoomArrábidaIIIChairedbyChristineWatson(SRUC,UK)16:30-16:40E.S.Jensen:Soilnitrogenfertilityandnitrogenacquisitioninfababean

16:40-16:50D.Savvas:Impactoforganicpracticesongrowth,yieldandgreenhousegasemissions16:50-17:00K.McPhee:Effectofsimulatedhailtreatmentonyieldlossinchickpea

17:00-17:10V.Verret:Meta-analysisoftheeffectsoflegumecompanionplants17:10-17:20S.Médiène:Atoolintegratingknowledgetoselectlegumespeciesforoilseedrapeintercropping17:20-17:30V. Sánchez-Navarro:Nitrous oxide andmethane fluxesfromacowpea-broccolicroprotation17:30-18:00 General discussion on Legumes andenvironmentSession 18, parallel: Resistance to biotic and abioticstresses-RoomArrábidaIVChaired by Weidong Chen (USDA-ARS, USA) and LaurentGentzbittel(CNRS,France)16:30-16:40 M. Dickinson: Exploring metabolic changes in legumesexposedtocombinedbioticandabioticstress16:40-16:50 K. Toyoda: The role of plant cell wall in resistance andsusceptibilitytopathogenicpathogen16:50-17:00 J.M. Osorno: Detecting tolerant germplasm and QTLSassociatedwithfloodingstressindrybean17:00-17:10S.Beji:Genome-WideassociationmappingoffrosttoleranceinPisumsativum

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17:10-17:20B.Ruge-Wehling:Marker-assistedbreedingstrategiesforanthracnoseresistanceinlupin17:20-17:30 D. Rubiales: Use of wild relatives in pea breeding fordiseaseresistance17:30-18:00General discussion on resistance to biotic andabioticstresses18:00-19:00Concludingsession-RoomArrábidaI&IIPosterandoralpresentationawardsILSHonorarymember’sawards20:00FarewellDinnerOctober15th2016(satelliteevents)08:00-12:00 REFORMA project meeting (partners only) -RoomDouro08:00-12:00 IYP_Research Strategy write shop (byinvitation)-RoomAtlântico

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KeyLecturers

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KeyLecture-Session112/10-09:15-09:45

HakanBahceci

HakanBahceciistheChairman&GroupCEOofHakanAgroDMCC,amultinationalagri-softcommoditiessupplycompanybasedinDubai,UAEwithprocessingfacilitiesandoffices in26countries.Hakanhasover 25 years’ of experience in global agri-soft commodities supplychain management. He currently serves as a Board Member ofGAFTA(GrainAndFeedTradeAssociation)andtheChairmanoftheTurkishBusinessCouncilinDubaiandChairofGPC'sIYPCommittee.Hakan notably served as President of Global Pulse Confederation(CICILS IPTIC)between2011-2015,asPresidentof InternationalAgriFoodNetworkandasChairpersonof thePrivateSectorMechanismat the UN Committee on World Food Security. Hakan holds aBachelor’sdegreeinEnglishLiteratureandanMBAfromWollongongUniversity,Australia.

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Forging a New Future for Pulses: Addressing researchchallenges with the momentum of the UN InternationalYearofPulsesBahceci,H

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KeyLectureSession212/10-11:00-11:30

Marie-HélèneJeuffroy

Marie-Hélène Jeuffroy is a senior scientist inAgronomy. Shedefendedher PhD in 1991 in ecophysiology, analysing and modeling yieldformationinapeacrop.Then,sheworkedonnitrogenmanagementinthe cropping systems, with the aim of designing new practices orideotypes taking into account various agronomic and environmentaltargets, and their use within the cropping systems. Then, she studiedparticipatorymethods for the design of decision-support systems and,more largely, technical innovations. A large part of her researchconcerned grain legumes, and their interactions with the croppingsystem.

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Valuing the environmental benefts of legumes requires aterritorialapproachJeuffroyM-H1,HellouG2,MagriniM-B3,PelzerE11 - UMR Agronomie INRA, AgroParisTech, Université Paris Saclay, 78850 Thiverval-Grignon,France2 - LEVA, Univ. Bretagne Loire, Ecole Supérieure d’Agricultures (ESA), 49007 Angers,France3-AGIR,UniversitédeToulouse,INRA,INPT,INP-EIPURPAN,Castanet-Tolosan,FranceDespite theenvironmentalbenefitsof legume-based cropping systemslargely described by scientists, and the great diversity of availablespecies and crop practices showing possible adaptation to localenvironment, legume areas have strongly decreased since the 90s inEurope. However, their use as feed or food is highly recognized asbeneficial for human health, nutritional qualities, and improvedEuropeanautonomyforprotein.Basedonsurveysaroundvariousstakeholdersatbothnationalandlocallevels, including farmers, the reasons for that discrepancy have beenidentifiedinFrance.Onemainreasonisthattheenvironmentalbenefitsare highly variable, according to the local situation, and farmers lackinformationand tools to take intoaccount this variability in their cropmanagement. Moreover, the benefits gained from legumes are mostoften measurable on the following crops, whereas the agriculturalsociotechnical system enhances annual assessment of practices.Another reason is that some environmental benefits address globalissues, which are not directly connected to the local stakeholdersconcerns.Theseobservationscallfortheneedofasharedvisionofthebenefitsgained from legumecropsamong thestakeholdersconcernedby theirpossibledevelopmentanduse.Wethusconclude thatvaluingthe environmental benefits of legume crops requires a territorialapproach.Basedonacombinedapproachmixingcasestudies in three territoriesandtheoreticalaspects,weshowthatgainingfromtheseenvironmentalbenefits requires combine technical, but also organisational andeconomicalinnovations,withintheterritory,combiningvariouslevelsoftheagrifoodsystem.Whenco-designedbytheconcernedactorswithina territory, these coupled innovations appear adapted to the localconditionsandstakeholders’activitiesandstrategies,and thusallowtoenhancethedevelopmentoflegumecrops,takingbenefitfromasharedvisionofthelegume-basedservices.

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KeyLectureSession312/1014:30-15:00

JensStougaard

JensStougaardisProfessorofMolecularBiologyandGeneticsatAarhusUniversityandDirectoroftheCentreforCarbohydrateRecognitionandSignalling (CARB). Jens Stougaard leads a group studying genesregulatingdevelopmentofnitrogenfixingrootnodulesandmycorrhizaformation in legumes. Currently the mechanisms of Nod-factorperception, the function of receptors involved and the downstreamsignaltransductioncascadesareinfocus.TheplantmodelsystemusedforthisresearchisLotusjaponicusthatisalsousedforinvestigatingthelong range signalling integrating root nodule development into thegeneral developmental program of the plant. Genetics, genomics andbiochemicalmethodsareusedtoidentifyandcharacterisecomponentsof regulatory circuits. In order to improve the genetic analysis and toestablish a system for reverse genetics, a large-scale insertionpopulation based on the germ-line specific activity of the LORE1retroelementisbeingestablishedandmadeavailabletothecommunity.

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ReceptormediatedsignalinginlegumesymbiosisStougaard,JCentreforCarbohydrateRecognitionandSignalling,DepartmentofMolecularBiologyandGenetics,AarhusUniversity,Denmark

In the model legume Lotus japonicus a large family of LysM receptorkinases are predicted to mediate plant-microbe interactions. Thefunction of some of these receptors in perception of microbial signalmolecules including lipochito-oligosaccharides, exopolysaccharides andchitinwillbepresented.Biochemicalapproachesforcharacterizationofligand – receptor recognition in relation to host specificity in legume-rhizobiainteractionswillbediscussed.

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Keylecture-Session412/10-14:30-15:00

NoelEllis

Thomas Henry Noel graduated with a B.Sc First class Honours inBiological Sciences (1976) and a Ph.D. (1979) with his thesis“ExaminationofthevariationofthestructureofsatelliteDNAs”.Since 2000 is was Head of the Applied Genetics Department (2000 –2001), Associate Head of the Crop Genetics Department, John InnesCentre (2001 – 2011), Professor of Crop Genetics, IBERS, at theUniversity of Aberystwyth (2011 – 2013) and Director, of the CGIARResearchProgramonGrainLegumes(2013–2015).Noel'sresearchinterestshavefocusedonthegeneticbasisoftheinter-and intra-specific evolution and diversification of legumes at bothgenomicandphenotypiclevels,developingapproachesfortheisolationofgenesidentifiedbytheirphenotype.Noel has promoted research networks through the EU FP6 integratedproject ‘Grain Legumes’ (2004-2008), theCGIAR’s activities in legumes(2013-2015)andhisactivitiesinAEPandILS.

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Whereareweafter150yearsoflegumegenetics?Ellis,N

Geneticresourcescomeinavarietyofforms,butcanbeclassedbroadlyinto designed or natural populations. Designed populations includesegregating individuals or lineages derived from controlled crosses,following Mendel's lead. Systematic mutant populations are anotherpowerful analytical tool and can provide useful allelic variation. Thesecan be interrogated either from the point of view of phenotypicvariation to determine the corresponding genotype, or using allelic(sequence)variation inorder todetermine theconsequentphenotypicvariation.Naturalpopulations,ontheotherhand,areoftenrepresentedby selections from wild material. There will be some structure in thewildmaterial, reflecting itspopulationhistory,and somestructurewillderive from the sampling strategy. For crop genetic resources wildmaterial isaugmentedbydiverselandracesandthecombinedmaterialisoftenheld inagermplasmcollection.Muchefforthasbeendevotedto understandpopulation structure in natural populations in thehopethat this can enable genetic analysis; essentially this is a surrogate fordesigningpopulationstructure.Thesegermplasmcollectionsarewidelyviewed as useful sources of allelic diversity, because the alleles theycontainhavesurvivedselection.This presentation will discuss the interrelationship between differentpopulation types for the characterizationof the genetic determinationoftraitsandemphasisethecontinuingroleofgeneticanalysisintheeraofextensivegenomesequenceinformation.

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KeyLecture-Session713/10-8:30-10:00

FrédéricMarsolais

FrédéricMarsolais is a Research Scientist at the London Research andDevelopment Centre of Agriculture and Agri-Food Canada (AAFC), andan Adjunct Research Professor at the Department of Biology of theUniversityofWesternOntarioinLondon,Ontario,Canada.Dr.Marsolaisgraduatedwith aB. Sc. inMicrobiology and anM. Sc. in Biology fromLavalUniversity,andaPh.D.inBiologyfromConcordiaUniversity.PriortojoiningAAFC,hewasanNSERCVisitingFellowatDukeUniversity,andtheUniversityofNorthCarolina atChapelHill.Dr.Marsolais’ researchfocuses on protein biochemistry in common bean, including proteinquality and seed protein composition. His laboratory also investigatesthemetabolismofsulphurandamideaminoacidsinrelationwithseedproteinaccumulation.

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Usingbeanswithnovelproteincompositionsfornutritionalimprovement.Marsolais,FAgriculture andAgri-FoodCanada. LondonResearch andDevelopmentCentre.

Aseriesofgeneticallyrelatedlinesofcommonbean(Phaseolusvulgaris)integrate a progressive deficiency in major storage proteins, the 7Sglobulinphaseolinandlectins.SARC1integratesalectin,arcelin-1,fromawildaccession.SMARC1-PN1 isdeficient inphaseolin.SMARC1N-PN1combinesadeficiencyinmajorlectins.Sanilacistherecurrentparentalbackground. The changes in protein composition lead to an increasedconcentrationofessentialsulfuraminoacids,methionineandcysteine,at the expense of the abundant non-protein amino acid, S-methylcysteine. Genomic research was performed to understand thebasis for variations in protein profiles. Reads obtained from nextgeneration sequencing technologies were aligned to referencesequences, and subjected to de novo assembly. The results identifiedpolymorphismsresponsibleforthelackofspecificstorageproteins,andthoseassociatedwithlargedifferencesinstorageproteinaccumulation,including compensatory changes. We also performed research toidentify genetic markers of phaseolin deficiency, map interspecificintrogressions fromP.coccineusanddeterminewhether the increasedconcentration of sulfur amino acids may be combined with goodagronomiccharacteristics.Variation inpectinacetylesteraseexpressionin the seed coat,whichmay affect theproperties of dietary fiber, hasalsobeencharacterized.

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KeyLecture-Session813/10-10:30-11:00

JudithBurstin

Judith BURSTIN is Director of research at INRAUMR1347AgroecologyDijon-France. The major goals of her program are to gain a betterunderstanding of the effects of pea genes that are relevant toagriculture and to develop the tools required for more efficient peaimprovement.Herresearchfocusesondecipheringthecontrolofseedyield and quality in the context of climate change. Her programintegrates a broad range of research projects that include wholegenome studies, mapping, positional cloning, and marker-assistedselection. Dr. Burstin has published >45 peer reviewed papers and 7book chapters. Recent accomplishments of Dr. Burstin’s research arethe development of genomic resources for pea, such as the peaGeneAtlasandahighdensityandresolution15kconsensusgeneticmap.These tools will serve for cloning the genes that control severalimportant traits in pea and for the establishment of the pea genomesequence. Dr. Burstin has led large consortiums of public-privatecollaborativeprogramsforthelast15years.

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TowardsthegenomesequenceofpeaMadouiM.-A.1,LabadieK.1,KreplakJ.2,AubertG.2,d'AgataL.1,CapalP.3,FournierC.2,KougbeadjoA.2,VranaJ.3,GaliK.K.4,TaranB.4,BelserC.1,LePaslierM-C.5,McGeeR.6,EdwardsD.7,BatleyJ.7,BendahmaneA.8,BergèsH.9,BarbeV.1,TayehN.2,KleinA.2,LichtenzveigJ.10,AuryJ-M.1,CoyneC.J.11,WarkentinT.4,DolezelJ.3,WinckerP.1,BurstinJ.21-CEA-Genoscope,Evry,France2-INRA,UMR1347Agroécologie,Dijon,France.3-InstituteofExperimentalBotany,Olomouc,CzechRepublic4-UniversityofSaskatchewan,Saskatoon,SK,Canada5-INRA,US1279EPGV,CEA-IG/CNG,EVRY,France6-USDA-ARS,Pullman,WA7-UniversityofWesternAustralia,Perth,Australia8-INRA,Evry,France9-INRA-CNRGV,CastanetTolosan,France10-CurtinUniversity,WA,Australia11-USDA-ARSWesternRegionalPlantIntroductionStation,Pullman,WA

Pea (Pisum sativum L.) was the originalmodel organism forMendel´sdiscoveryofthelawsofinheritanceandkeptthismodelstatusuntiltheadventofmolecularbiologyattheendofthe20thcentury.Pea isalsoone of theworld’s oldest domesticated crops. It is currently the thirdmostwidelygrownpulsecrop,as itsseedsserveasaprotein-richfoodforhumansandlivestockalike.While several legume species genome's draft sequences have beenproduced, progress in pea genomics has lagged behind largely as aconsequenceof itscomplexandlargegenomesize.Thepeagenomeislarge (ca 4.45 Gb), probably resulting from recent expansion ofretrotransposonsfollowedbysequencediversification.The Pea Genome International Project has undertaken severalcomplementary strategies in order to produce a high-quality draftsequenceofthespecies.Wewillpresenthowthisdraftsequenceopensthewaytorenewstrategiesinpeabreeding.

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KeyLecture-Session1113/10-16:30-17:00

PhilMullineaux

PhilMullineauxobtainedhisPhDfromtheUniversityofWales in1981andwasapostdoctoral fellow, firstat theUniversityofEdinburghandthe John Innes Institute (now the John Innes Centre) until 1986. AfterthishewasappointedasaGroupLeaderandfromthistimeembarkedon studying the response of plants to environmental stress and theirassociated subcellular and systemic signalling especially the role ofreactive oxygen species and antioxidants. Philmoved as Professor ofPlantMolecularBiologytotheUniversityofEssex in2004,primarilytoexpandhis experience of plant physiology. Thework has continued atEssex and expanded to trying to understand the basis of the balancebetween growth and stress defence that every plantmust achieve. Inthe context of this Congress, Phil has been and is a contractor inEuropean Union FPV –FPVII projects (AIR1-CT92-0205 [ESTIM], FOOD-CT-2004-506233 and FP7-KBBE-2011-289562 [ABSTRESS] respectively).Therefore he has considerable experience of projects with bothacademicand industrialpartners.HiscurrentEUproject (ABSTRESS)ofwhich he is a partner aims to understand the interaction betweendroughtstressandFusariuminfectioninlegumesandothercropspeciesandhasstrongcropgenomicsandBayesianmodellingcomponents.Hismany publications can be found on his Google Scholar page(https://scholar.google.co.uk/citations?hl=en&pli=1&user=_yM2sFkAAAAJ)

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Theidentificationofnovelgenescontrollingplant-environmentinteractionsMullineaux,PSchoolofBiologicalSciences,UniversityofEssex,UK I will report an approach we have been using in the FP VII projectABSTRESS inwhichwehaveused time series transcriptomicsdataanddynamicmodellingmethods to identify candidate genes in themodellegumeMedicagotruncatula(Mt)thatuniquelyrespondtoacombineddrought stressandFusariumoxysporum infectionandmove rapidly topea.ThisisbuiltonamethoddevelopedandpublishedforArabidopsis.Thedynamicmodelling,inanunbiasedway,allowsustoidentifygeneswith high numbers of connections to other genes in inferredtranscriptional networks. Such genes, therefore, may be of moresignificant interest than genes that are only differentially expressed inresponsetothecombinedstresses.Thesearetermedhubgenesandweidentified 36 such candidates inMt. The orthologs for 22 of theseMtgeneswareidentifiedinpeaandweconfirmedfor11ofthemthatthepeagenesrespondedmorestronglytothecombinedstressthaneithersinglestress.Mutantsinthese11peahubgeneorthologshavebeen,orarebeing identifiedby TILLING,with the aimofdetermining if alteredphenotypesofthepeamutantstoFusariumand/ordroughtinfectionismanifestedunderfieldconditions.Therefore,ABSTRESShasbeentakinganovelapproachtoidentifyinggenesinmodelspeciesanddeterminingiftheircounterparts incropspeciescanbefast-trackedthoughtofieldtesting and eventual incorporation into breeding programmes. Thiscommunicationwillreportourprogresstodate.

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KeyLecture-Session1213/10-16:30-17:00

SeidAhmedKemal

Seid A. Kemal is a Legume Pathologist in the Biodiversity andIntegrated Gene Management Program at ICARDA Morocco,ResearchPlatformworkingwithteamsoffoodlegumebreeders,IPMexpertsandAgronomists.HereceivedhisBScdegree(inAgriculturemajoringPlantsciences)fromAddisAbabaUniversityandMSc(CropProtection)degreefromAlemayaUniversityofAgriculture,Ethiopia.He earned his PhD degree from the Department of Biology,University of Saskatchewan, Canada. He has served as Lecturer inCropProtectioninAlemayauniversityofAgriculture.Hewasleadingthe National Pulse Research Team in the Ethiopian institute ofAgricultureResearchandcontributedinthereleasesofhighyielding,disease resistant kabuli chickpea and lentil in Ethiopia. His majorareas of interest are developing resistant sources to foliar and soilborne diseases of food legumes; disease epidemiology andintegrated pest management. He authored and co-authoredscientific articles on diseases of cool season food legumes andsupervisedandsupervisedover20graduatestudents.

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Integrateddiseaseandinsectpestmanagementpestandincool-seasonfoodlegumesAhmed, S Biodiversity and Integrated gene Management, International Center for Agricultural Research in the Dry Areas, Rabat, Morocco

Cool-seasonfoodlegumes(fababean,chickpea,fieldpeaandlentil)arekey commodities in cereal-legume mixed cropping system in NorthAfrica,WestandSouthAsiaandEastAfrica.Thecropsarekeysourcesofprotein; incomes,animal feedand improvesoil fertility tosmallholderfarmers.Themajorbioticfactorsaffectingcropyieldarefungaldiseases,virus, parasiticweeds and insect pests. New diseases and insect pestsare emerging due to climate variability and farming system changes.Major investment are being made in developing resistant cultivars inmany countries.Otherpestmanagementoptionare culturalpractices,bio- fumigation, application of pesticide and bio-pesticides andintegrationoftwoormoreoptionstomanagepeststoreduceyieldandquality gaps in small holder farmers. The development of resistantcultivarsisnotpossibleformostpathogensandinsectpestsduetowidehost.Integratedpestmanagement(IPM)ismainlyappliedtopestswithwide host ranges and cultivars with partial resistance. IPM has manybenefitsfarmersbyincreasingyield,reducingcostsofproduction,delaydevelopment of more aggressive pathogen populations and reduceenvironmental and health hazards of pesticide uses. IPM options aredemonstrated through different platforms (Farmer Field Schools,Innovation platforms, etc) where farmers and other stakeholders areinvolvedtoimprovetheirknowledgeandskills.Inthispresentation,thestatusofIPMresearchandadoptioninmanagingtheimpactofpestsoffoodlegumecropsofsmallholderfarmersarereviewed.

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KeyLecture-Session1314/10-08:30-09:00

ScottA.Jackson

ScottA. Jackson is theGeorgiaResearchAllianceEminentScholarandProfessorofPlantFunctionalGenomicsattheUniversityofGeorgia.HeistheDirectoroftheCenterforAppliedGeneticTechnologiesandhasappointmentsintheInstituteforBioinformatics,PlantBiologyandCropandSoilSciences.Hisresearchfocusesontheapplicationofgenomicsfor crop improvement and the understanding of basic biologicalprocesses,suchaschromosomeandgenomeevolution,domesticationandpolyploidy.His labworks primarilywith rice and several legumesand is international in scope with work in Asia, Africa and SouthAmerica. His lab has generated more than 135 publicationsencompassingbasictoappliedaspectsofplantandcropgenetics.

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GeneticandEpigeneticVariationinLegumesandtheirRoleinImprovementJacksonS.GeorgiaResearchAllianceandUniversityofGeorgia

Epigenetic variation, non-sequence-based variation, has been of greatinterestinplantandanimalresearch.Usingsoybeanandcommonbean,weareexploringthelevelofepigeneticvariationinbreedinggermplasmas well as association panels. Using sodium bisulfite DNA sequencing,Methyl C sequencing, we have sequenced more approximately 200soybean and common bean accessions including landmark cultivarsfrom thepast 80 yearsof soybean improvement in theUS, parentsofthe public soybean NAM (nested association mapping) population,landraces,acommonbeanGWASpanelandundomesticated relatives.Using these data, including RNA-seq and small RNAs, we determineddifferentially methylated regions (DMR) for all three methylationcontexts(CG,CHGandCHH).TheseDMRswerethenanalyzedfortheirgenomic context: exonic,UTRs,upstream,downstream,and intergenicor repeat (e.g. transposable elements). I will discuss these data incontextofDMRvariation (inheritedanddenovo)within andbetweenspecies,varieties, landracesandwildsoybeanaswellastheirpotentialcontribution to breeding/selection over the past 80 years and theircontributiontovariation.

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KeyLecture-Session1414/10-10:30-11:00

EricJustes

EricJustes(Male,bornin1965,SeniorresearcheratINRA,France),isanagronomistandmodellerspecialisedinsoilscience,ecophysiologyandagroecology with experience in legume insertion in arable croppingsystems as sole or intercrops, i.e. species mixtures of cereal-grainlegumes and catch/cover crops. His expertise covers water andnitrogen cycling, management in arable systems and design ofinnovativelowinputs,organicanddiversifiedcroppingsystems.Hehaspublished over 60 articles in ISI journals(https://www.researchgate.net/profile/Eric_Justes). He is currentlyheadof theVASCOresearch team(VArietiesandCroppingSystemforanagrOecologicalproductionatINRA,AGIRlab,Toulouse),coordinatorof French and European projects and is involved in disseminationactivitiestoagriculturaladvisors.

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Synthesisontheeffectsofgrainlegumeinsertionandcereal-grainlegumeintercropsinlowinputcroppingsystemsinSouthernFranceJustesE.,Plaza-BonillaD.,GavalandA.,LéonardJ.,MaryB.,NolotJM.,PerrinP.,PeyrardC.,RaffaillacD.Six cropping systems (CS) of three 3-year rotations based on durumwheat and sunflower inserting 0, 1 (pea or fababean) and 2 grainlegumes (GL) (peaandsoybean),andwithorwithoutcovercrops (CC)werecomparedatINRAToulousefrom2004.Thisexperimentisstillongoingforatwelfthyear.Wedemonstratedthat6keypoints.1)Peaasapreceding crop increased durum wheat grain production by 8%comparedtosunflowerasaprecedingcropwithameanreductionofNfertilizationof45kgNha-1.2)InsertingGLintherotationssignificantlyaffectedtheamountofCandNinputstothesoilthatwerelowerthanwithcerealsandconsequently ledtoadecrease insoilorganic-C(SOC)and –N contents. 3) N leaching simulated using the STICS model washigherwhenincreasingthenumberofGL(from22to52kgNha-1aftertworotationcyclesof6years, for0 to2GL respectively).4)However,CCinsertioni)reducedNleaching(from15to18kgNha-1),ii)mitigatedSOCloss,andiii)didnotaffectdurumwheatgrainproteinconcentrationor yield. 5) Daily measured N2O emissions over the whole 3-yearrotation were low but significantly higher under the CS includingfababeanthanforthecereal-basedCS(1.12vs.0.78kgN2O-Nha

-1year-1) despite a lower N fertilization. Then, in such conventionally-tilledsystems,properlydesignedcroppingsystemsthatsimultaneouslyinsertgrain legumesandcovercropsreduceNrequirementsofthefollowingdurumwheat,stabilizeSOCcontentbutdonotdecreaseN2Oemissionsattherotationlevel.

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SessionAbstracts

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Session 1, plenary: Legumes value chain: marketrequirementsandeconomicimpact-RoomArrábidaI&II

Chaired by Eduardo Rosa (UTAD, Portugal) and AdrianCharlton(FERA,UK)

09:15-09:45 Key lecture - Hakan Bahceci: Forging a NewFuture for Pulses: Addressing research challengeswith themomentumoftheUNInternationalYearofPulses

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OralCommunications

09:45-10:00 Oral–S1

Main-streamingpulses:exploringlocalsolutionstosupplychainlimitationsBlackK.1,2,WalkerG.2,WhiteP.1,KarleyA.1,RamsayL.2,RamsayG.3,JamesE.1,SquireG.1,IannettaP.1.1 - Ecological Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA,ScotlandUKpete.iannetta@hutton.ac.uk2-YeastResearchGroup,AbertayUniversity,DundeeDD11HG,Scotland3-TayBeesandHoneyLtd.-www.taybees.co.uk

IntheUK,peasandfababeansarethemainpulsesproducedandtheircommercial success needs improved if they are to compete moreeffectively in themarketplace.Onesolution is tousepulsesasa rawmaterial for higher value products. For example, UK farmed salmonproduction is 160,000 T/ywith a farm gate value of £600m and feeduse is set at 200kT/y. Production is set to increase dramatically andpulse protein sources are in demand. However, feed manufacturesutilisetheproteincomponentandotherkeyaspectsoftheUKssupplychainarelimitinguptakebyaquaculture.Beandehullersandmillersarenotco-localisedandcentralisingprocessingwouldimprovecommercialefficiency; processing facilities to fractionate locally grownpulses intotheircomponentstarchandproteinportionsremaintobeestablishedin the UK, and; the value achieved for the starch byproductmust bemaximisedasitaccountsofthebulk(ca.60%),ofthepulsebyweight.However,wereportonnovelprocessingsteamsforwhole fababeansin brewing and distilling, and the development of byproducts foranimal- and aquaculture-feeds. The UKs brewing and distillingindustriesareveryimportanteconomicallyat£10billionannuallyintaxrevenues;avaluesecondedonlybyfarmedsalmon.CanourapproachhelpalignmajorUKsupplychainsandtheperceptionofpulse-useasaculturalnorm?Acknowledgements-TheScottishGovernment,EU-FP7LegumeFutures,InnovateUKseewww(dot)beans4feeds(dot)net.

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10:00-10:15 Oral–S1113Wouldtheproteinfractionbethefutureofoilandgrainlegumecropsby2030?MuelF.,PilorgéE.Terres Inovia, Institut Techniquedesoléagineux, desprotéagineuxetdu chanvre.Av L.Brétignières,F-78850Thiverval,Grignon,France,f.muel@terresinovia.fr

TosupportthereflectionsoftheFrenchprofessionalsofvegetableoilsandproteins,aprospectivestudywascarriedout to2030horizon (15years), to shed light on the opportunities that will draw the oil andgrain legume crops, and the areas of growth for the French andEuropeanvegetableoilandproteinsector.Thethinkingwasorganisedin the form of four different scenarios for 2030, which illustratedifferentlogicalevolutionsofthecontextandrelatedkeyissues,underthepressureofdemographic,economicandsocio-politicalconstraints.Most of these scenarios seem favorable to the development of grainlegumes in Europe, and it is clear that the economic value of theprotein fraction is a key aspect of the future of grain legumes andoilseeds.Moreover, theEUmightbeable tobecome self-sufficient inproteinconsumptionforbothfeedandfood.

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10:15–10:30Oral-S1LegumefuturefromEuropeanUnionperspective:Horizon2020,EIP-AGRIandCAPDuboisG.EuropeanCommission-DGAGRI

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PostersP1–S1Doconsumers'valuethenewuseoflegumes?Anexperimentalauctionwithlegumefortifiedmaizebread.SeabraPintoA.1,BritesC.1,VazPattoC.2,CunhaL.31–InstitutoNacionaldeInvestigaçãoAgráriaeVeterinária,INIAV,Oiras,Portugal2 – Instituto de TecnologiaQuímica e Biológica, ITQB,UniversidadeNova de Lisboa,Oeiras,Portugal3-SensetestLegumes have a significant potential for a more sustainable andhealthyfood.Pulses,whenblendedwithcerealproteins,mayofferapromising alternative source for nutritional and functional proteins.Inthiscontext,manufacturersperceivedthatconsumersaremakingmoreconsciousandhealthfulfoodchoicesandtheyaremovingawayfrom artificial ingredients and towards introducing products high indesirable attributes like fiber and protein. Environmental and socioeconomic concerns also influence purchase decisions. Indeed,consumers believe that health and environmental attributes areimportant, but are they willing to pay more for the benefits theyprovide? Under the research work in experimental economicsconsidered in the European Project LEGATO an experimentalprocedure was used to elicit consumers’ willingness to pay withregard to legume fortified maize bread “Broa”. This product isspecific Portuguese maize bread, very well-known and eatenseparately during the meals. A sample of Portuguese consumersparticipated in an experimental auction and they evaluated fivedifferent breads in three different treatments. The incentivecompatible elicitation mechanism used was the original SurplusComparisonMechanism(SCM)thattakes intoaccounttheinfluenceofthediversityofproductsactuallyavailableonthemarketandtheircomparison. This study contributes to a better understanding ofconsumers’perceptionsandtheirbehaviours regardingnewusesoflegumes.

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P2–S1Establishment of a knowledge transfer network forcultivationandutilisationof fieldpeas and fieldbeans inGermany–DemoNetErBoJacob I.1, Vogt-KauteW.1, Stevens K.2, Zerhusen-Blecher P.2, QuendtU.31-NaturlandFachberatung,Eichethof1,85411Hohenkammer,Germany2-UniversityofAppliedSciencesSüdwestfalen,LübeckerRing2,59494Soest,Germany3-HesseDepartmentofAgricultureAffairs(LLH),KölnischeStraße48,34117Kassel,GermanyStarting inMarch2016,aknowledge transfernetworkconsistingof75conventionalaswellasorganicfarmsgrowingfieldpeas(Pisumsativum)or field beans (Vicia faba) is currently established in Germany.Cultivationofpeasandbeanshasbeenonarather lowandstagnatinglevelinGermanyinthepastyears.Thisisduetosomechallengesduringcultivation, but also to low economic incentive for the farmers. Thisnetwork (DemoNetErBo) shall expand and enhance the cultivation aswellastheusageoffieldpeasandfieldbeanssustainablyandimprovethevaluegainedbythefarmerswhengrowingpeasandbeans.Specialfocusisthereforeonthedevelopmentandpresentationoflegumevaluechains for feed and food production spanning all levels frombreedinguntil the usage by the consumer.Hence, the farms are demonstratingdiversebestpracticeexamplesforcultivation,processing,andutilisationofthosetwograinlegumes.Theoverallaimofthisprojectistomeetthegrowingneedforregionallyproducednon-GMOproteincrops.Theproject is supportedby fundsof theFederalMinistryof FoodandAgriculture(BMEL)basedonadecisionoftheParliamentoftheFederalRepublic of Germany via the Federal Office for Agriculture and Food(BLE)undertheFederalProteinCropStrategy.

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Session2,plenary:Legumesandenvironment-RoomArrábidaI&IIChaired by Richard Thompson (INRA, France) and DiegoRubiales(CSIC,Spain)11:00-11:30Keylecture-Marie-HélèneJeuffroy:Validatingtheenvironmentalbenefitsoflegumesrequiresaterritorialapproach

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OralCommunications11:30-11:45 ORAL–S2EnvironmentalimpactofintroducinglegumesintocroppingsystemintemperateregionsCupinaB.1,KrstcDj.1,AntanasovicS1.,MikicA.2,EricP.11-UniversityofNoviSad,FacultyofAgriculture2-InstituteofFieldandVegetableCropsTheuseof legumes in cropping system isonemeasure thathasbeentaken in agricultural production to increase environmental protectionand toencourage sustainableuseofnatural resources. Legumesoffermanybenefitstosustainableagricultureandtheintegrationoflegumesinto cropping systems brings costs and benefits, both internal andexternal to the farm. For ecological reasons, legumes are gainingincreasingimportance.Legumecanbeeasilyincludedinacroprotationand,incontrasttograssesandcrucifers,contributeadditionalNtothenutrientcyclebysymbiosis.SomeofthisNcanbeusedlaterasanimalfeed in the form of protein in herbage while the rest of theaccumulatedNcanbe takenupby subsequent crops.On thebasisofexperiments conducted in Serbia it can be concluded that themanagement decision concerning the use of legumes as cover cropsshould be based on the balance between farm profitability andenvironmental sustainability.Theeffectof legumesoncashcropyieldandquality ismainlypositive.Nmineralizationshouldbe regulated inaccordance with the N demands of the subsequent crop. In animalproductionareas,legumescanbeanimportantsourceofqualityforageorcanbeusedformulching.InsuchcasesandinrotationwithcornorSudangrass,legumesshouldfulfilthefollowingrequirements:low-costproduction, yield and quality, N uptake during periods critical forleachingandnonegativeeffectsonsubsequentcrops.

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11:45-12:00 Oral–S2Sustainablemanagementofgrass-whitecloverleysinley-arablefarmingsystemsWatsonC.A.1,2,WalkerR.L.,ReesR.M.,ToppCFE21-SLU,Sweeden2-SRUC,SweedenIn ley-arable systems, grass-clover leys provide symbiotically fixednitrogenwhichisreleasedthroughmineralizationtosupplyNforothercropsintherotation.ThemanagementoftheleyiscriticalintermsofbothNfixedandherbageyieldandquality.HereweaddresstheimpactofageofleyonNfixationandclovercontentandtheimpactofpHonclover content. We report measurements in two long-term croprotation experiments in the north-east of Scotland. An organicallymanaged crop rotation experiment established in 1991 and aconventionallymanaged rotationexperimentestablished in1961withpH treatments ranging from 4.5 to 7.5. The differences in nitrogenfixation resulting from the ageof the ley (age 1, 2, and3) havebeenassessed over four years. The total above ground N fixed was in theregionof72–156kgN/yr.TheresultsindicatethattherateofNfixationis affected by the interaction between the age of the sward and thesample time, and the year and the sample time. The age 3 swardstendedtohaveahigherrateof fixationat thestartof theseasonbutwhichdeclinedearlier intheseasonthaninswardsage1and2years;however, it isnotclearfromthesedata if totalNfixation increasesordecreaseswiththeageofley.

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PostersP3–S2BiologicalNitrogenFixation:AdoptionratesoflegumetechnologiesamongsmallholderfarmersinNorthernGhanaandenablingfactorsthatinfluencefarmers'decisiontoadoptKwatengG.,SvenssonS.Dept.ofBusinessandEconomics,SwedishUniversityofAgricultureSciences,UllsVäg28,P.O.BOX7012,75007Several studies have been conducted to understand the adoptionpotentialoflegumetechnologyandfactorsthataffectadoption(SheltonandFranzel,2005;MwangiandKariuki,2015).ThestudybyMwangiandKariuki (2015) reveals that institutional factors,humanspecific factors,technological factors and economic factors have great potential toimproveadoptionamongsmallholderfarmers.AstudybyMafongoyaetal. (2006) suggest that, improving agriculture yields is building andmaintaining soil fertility in spite of low income and labor constraintfaced by smallholder farmers. Similarly, a review by Adjei-Nsiah et al.(2007)showthat legumesareableto improvesoil fertilityduetotheirabilitytofixN2andproduceN-richresiduesthatmayberichtothesoil.The effective ways in restoring such depleted soil is by improvinginorganicfertilizersandmanuretoovercomenutrientdepletion(Snappetal.,2010).However, theseproductsarenotreadilyavailable forusebysmallholderfarmers.Atthesametime,Sanchez(2002)estimatesthehigh cost of inorganic fertilizers in Africa to be two to six timescompared to the cost in Europe,NorthAmerica, orAsia. This is partlytrue in the caseofGhanawhere subsidies havebeen removedby theGovernmentofGhana(Adjei-Nsiahetal.2008)

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P4–S2 ThemultifunctionalroleoflegumesinvineyardsandorchardsVymyslickyT.1,PavlousekP.21-AgriculturalResearch,Ltd.,CzechRepublic.2-MendelUniversityinBrno,CzechRepublic.Legumeshavemanypositiveecologicalrules.Invineyardsandorchardsthey eliminate soil and wind erosion and serve as feed source forpollinators, insectandanimalspecies.Maybethemost importantroleistheirabilitytofixatmosphericnitrogenandsupplyotherplantswithnitrogen.Purelegumestandisabletofixupto200kgofNperhectare.Within a research project “Different ways of vineyard greening andmanagement and their influence on reduction of soil erosion andquality of production” the role of legumes have been studied in sixselectedvineyardsinthesouth-easternpartoftheCzechRepublic.Themainaimoftheprojectisdevelopmentandtestingofnewcovercropslegume mixtures intended for use in vineyards both in organic andintegratedproduction.Species-richmixturesbothforinter-rowsandforspace under grape plants serve to promote biodiversity in vineyards.Cover cropsmixtures have a positive effect on physical and chemicalsoilproperties,growthanddevelopmentofthegrapeplants,yieldandquality of grapes and wines. At the end of the project the finalcompositionoftestedmixtureswillbespecifiedconsideringspecificsoilandclimaticconditions.Suitabletechnologiesofplantingandasystemof stand treatments including the abovementioned positive effect oflegumes, as atmospheric nitrogen fixators, on the consumption ofnitrogenous fertilizers and thus effective management of mineralnutritionwillbeemphasized.

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P5–S2EnvironmentalimpactofintroducinglegumesintocroppingsystemintemperateregionsCupinaB.,VujicS.,KrsticD.,MikicA.,EricP.UniversityofNoviSad,FacultyofAgriculture,NoviSad;InstituteofFieldandVegetableCrops,NoviSadThe use of legumes in cropping system is onemeasure that has beentaken in agricultural production to increase environmental protectionand to encourage sustainable use of natural resources. Legumes offermanybenefitstosustainableagricultureandtheintegrationoflegumesinto cropping systems brings costs and benefits, both internal andexternal to the farm. For ecological reasons, legumes are gainingincreasingimportance.Legumecanbeeasilyincludedinacroprotationand, incontrasttograssesandcrucifers,contributeadditionalNtothenutrientcyclebysymbiosis.SomeofthisNcanbeusedlaterasanimalfeedintheformofproteininherbagewhiletherestoftheaccumulatedN can be taken up by subsequent crops. On the basis of experimentsconductedinSerbiaitcanbeconcludedthatthemanagementdecisionconcerning theuseof legumesas cover crops shouldbebasedon thebalance between farm profitability and environmental sustainability.Theeffectoflegumesoncashcropyieldandqualityismainlypositive.Nmineralization shouldbe regulated in accordancewith theNdemandsofthesubsequentcrop.Inanimalproductionareas,legumescanbeanimportantsourceofqualityforageorcanbeusedformulching.InsuchcasesandinrotationwithcornorSudangrass,legumesshouldfulfilthefollowingrequirements:low-costproduction,yieldandquality,Nuptakeduring periods critical for leaching and no negative effects onsubsequentcrops

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P6–S2 GeneticStudiesofWinterHardinessinPea(PisumsativumL.)HoldtC.,McPheeK.NorthDakotaStateUniversity,USAProductionofdrypeahasincreasedinNorthDakotaandotherstatesinthe Great Plains due to their high protein content and ability tosymbiotically fixatmosphericnitrogen.Fall sowingallows thepeacropto avoid high summer temperatures during the bloom period andincrease seed production. Fall-sownpea cropswould benefit northernstates,however;extremecoldwinter temperatures limit their success.This research aims to aid the development of winter hardy peas bydeveloping improvedscreeningmethods to identify increased levelsofwinter hardiness. An RCBD with six and four replicates was used toevaluate62germplasm lines and160RILs, respectively. Twoweekoldseedlingswere acclimated for fourweeks at 4°C and subjected to thefreezing cycle. Temperatures rampeddown from3°C to -8°C at 1°C/hrand increased at the same rate after a one hour freeze at -8°C.Individualplantsweregivenaninjuryscoreeverythreedaysduringthe21 day recovery period and Area Under the Injury Curve values werecalculated. Phenotypic data for freezing response will be used toconductQTLanalysis using a SNPbasedmapof the Shawnee/Melrosepopulation. Statistically significant differences were detected (P<0.01)among the 62 germplasm lines. Results of the QTL analysis will bepresented.Developmentof improvedprotocolsandmolecular tools todetect winter hardiness in controlled environment will increase theproductivityofbreedingprogramsfocusedonwinterpeas.

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P7–S2 Soilcompactionevaluationinno-tillageplotsunderwheat-chickpearotationMasmoudiY.A.InstitutNationaldelaRechercheAgronomique(INRA),126,RueMoulayIsmailDerbOmar,Settat,MorrocoThis work consists on evaluating compaction of soil under no-tillagesystemandwheat-chickpea rotation system. Thepenetrationpressureanddepthpressureweretakenusingportablepenetrometer(30°cone).Measurementsweredoneinthemiddleandextremitiesofthetrial.Asreference, compaction was done in adjacent field practiced usingconventional tillage. On field measurements (in the number of three)weremadebetweenJanuaryandmarch2016accordingsoilstate,withdifferentsoilwatercontent(dry,medium,wet).Theresultsshowedanincreasing of penetration forces according to the maximum depthsreached from the three measurements (the middle, extremities andreference)arerespectivelylocatedbetween[13-38cm],[21-52cm]and[18-50 cm]. However, the depths recorded in the extremities of thesameplotwere respectively small [3-19cm], [4-24cm]and [7-25cm].Penetration depths recorded in the conventional plots are locatedrespectively between [20-74 cm], [16-89 cm] and [20-75 cm]. Theseresultsshowedthatthereisasignificantcompactionintheextremitiesof no-till plots, regardless of the state soil moisture also there is theimportantnumberofpassagesmadeby theagricultural tractorswhentheyturnontheplots.

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P8–S2Fumonisinsassociatedwithcowpea(Vignaunguiculata):OccurrenceandphytotoxicityKritzingerQ.1,AvelingT.A.S.1,2,KotzeR.G.1,CramptonB.G.1,21 - Department of Plant and Soil Sciences, University of Pretoria, Pretoria 0002, SouthAfrica.2-ForestryandAgriculturalBiotechnologyInstitute,UniversityofPretoria,Pretoria0002,SouthAfrica.quenton.kritzinger@up.ac.zaResearch regarding mycotoxins and cowpea is a relatively unexploredfield, especially in South Africa. In 2003, the natural incidence offumonisins in cowpea seed was reported for the first time with totalfumonisin levels ranging from 0.12-0.61 µg/g. Additional samplesshowed levels of 7.85–56.50 µg/g. This paper reports on our researchactivities regarding the occurrence of fumonisins on cowpea, withemphasisonthephytotoxicnatureoffumonisinB1(FB1).Wefoundthatgermination and emergence of seeds imbibed in various FB1concentrationsweresignificantlydecreased.Seedlingmasswasreducedby all FB1 concentrations. Chlorophyll contentwas higher in leaves ofseedlings raised from seeds imbibed in FB1 when compared to thecontrols. Transmission electronmicroscopy of FB1 treated seed tissuerevealed ultrastructural deteriorations including compaction of thecytoplasmandseparationoftheplasmalemmafromthecellwall.FB1isknowntoinhibittheformationofsphingolipids,andwethereforetestedwhetheritwouldhaveaneffectonexpressionoftheceramidesynthasegene, the product of which plays a role in sphingolipid biosynthesis.However, semi-quantitative reverse transcriptase PCR suggested thatthere were no significant differences in ceramide synthase geneexpression between control and FB1 treated cowpea root and shootsamples. Contaminated cowpea seed could affect food security, thusappropriatepreventionandcontrolstrategiesshouldbedeveloped.

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P9–S2 Analysisofwildpea(Pisumsativumsubsp.elatius/humile)seeddormancylevelsHýblM.1,KopeckýP.1,PechanecV.2,BrusJ.2,TokerC.3,SmýkalP.41 - CropResearch Institute, TheCentreof theRegionHaná forBiotechnological andAgriculturalResearch,Olomouc,CzechRepublic.2 - Department of Geoinformatics, Palack_ University in Olomouc, Olomouc, CzechRepublic.3-DepartmentofFieldCrops,AkdenizUniversity,Antalya,Turkey.4-DepartmentofBotany,Palack_UniversityinOlomouc,Olomouc,CzechRepublic.Seed dormancy and germination characteristics may vary withinspecies in response to several environmental factors. In the wild,manyseedswillonlygerminateaftercertainconditionshavepassed.The objective of the study is to understand the genetic basis ofadaptation to environmental conditions influencing dormancyreleaseandthetimingofseedgermination,asacriticalmomentinaplant’s life, with fundamental consequences on fitness, using wildpea (Pisum sp.) model. Fifty geographically different wild pea lineswerescoredatfluctuatingtemperaturesof40/15°C,30/10°C,20/5°Cregimes and variation in dormancy was recorded. In addition, 20selected genotypes were tested for germination in semi-naturalconditions of eastern Turkey. Similarities as well as differences tolaboratory results were found, possibly related to soil temperatureoscillations and rainfall pattern. These lines were also analysedanatomically forseedcoatstructureusingSEM, lightmicroscopyoncryosections and seed surface imaging. Simultaneously selectedbioclimatic, topographic and humidity conditions will be extractedbased on geographical coordinates from available layers. For eachattributewillbegenerateddescriptivestatisticsandcalculatedbasicregression relationship. Results be subjected to model testingrelationshipwithdormancylevels.Acknowledgement: funding originates from Grant Agency of CzechRepublicprojectNr.16-21053S.

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P11–S2 AgronomicperformanceofwhiteclovergenotypesinsoleandmixcroppingwithryegrassandchicoryHeshmatiS.,IsselsteinJ.Georg-August-UniversitätGöttingenWhiteclover (Trifoliumrepens L.) is recognizedas themost importantforage legumeinthetemperatezonesoftheworld.Thecultivationofclover inmixturewithryegrass(Loliumperenne) isacommonpracticebecause there are a number of potential advantages of mixtures ascompared to monocultures. The inclusion of a forb as a thirdcomponentmayprovidefurtherproductionadvantages,inthepresentinvestigation the hypothesis was tested that the performance ofmixtures is dependent on growth characteristics of white clover thatvaryamongdifferent clover genotypes. Eightnovel genotypesofwhitclover were grown on two sites either as monoculture or as binarymixtureswithryegrassandorchicoryorasmultispeciesmixtureswithryegrassandchicory.Herbagewasharvested two times in thesowingyear and four times in the first main production year. The mixturesproduced high-accumulated total dry matter yields than the clovermonocultures. The multi species mixture was not better performingthan the binary mixtures. For the accumulated yield no significanteffectofthefactorclovergenotypeandtheinteractionclovergenotypex cultivation was found. However, if single harvest were considered,clover genotype showed significant effects. A detailed analysis of thegrowthcharacteristicsofthewhiteclovergenotypesisonthewayandisintendedtoenabletheidentificationofclovertraitsthatarerelevantforthemixtureperformance.

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P12–S2 Effectofenvironmentontotalphenolandtannincontentandtheirrelationshipwithotherseedcomponentsinsoybean[Glycinemax(L.)Merrill]genotypesJhajjK.K.1,GillB.S.1,SharmaS.21-DepartmentofPlantBreedingandGenetics,PunjabAgriculturalUniversity,Ludhiana-141004,India2-DepartmentofBasicSciencesandHumanities,PunjabAgriculturalUniversity,Ludhiana-141004,IndiaSoybean [Glycine max (L.) Merrill] is an important agricultural anddietarycommodityworldwide.Themostimportantorganiccomponentsofsoybeanseedareproteins(about40%)andoil(about20%).Withtheexception of isoflavonoids, few studies have been carried out on theotherphenolicclassespresentinsoybean.Thepresentinvestigationwascarried out to study the effect of environmental conditions (differentphotoperiod and temperature regimes) on the presence of phenoliccompounds insoybeangenotypes.Seventeensoybeangenotypesweregrownunderthreedifferentplantingdatesi.e.March1(D1),June5(D2)andAugust7(D3)toexposeseeddevelopmentstagestothreedifferentenvironmental conditions. Pooled analysis of variance over the threeenvironments (sowingdates) showed thatgenotypes, sowingdateandgenotype x sowing date interactions were significant (P < 0.01) forphenolandtannincontent.Bothtotalphenolandtannincontentwerefound tobe lowest in caseofMarch sowing (D2)withaverageof27.1mg/g and 12.4 mg/g, respectively which implied that when seeddeveloped under high temperature and photoperiod, the phenolcontent was low as compared when seed filling takes place at lowtemperature and photoperiod. Correlation coefficients of bothcomponents with some other seed quality characters were alsoestimated. Mild to strong significant associations were observed forsomeofthenutritionalandantinutritionalseedcharacters.

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P13–S2 MineralizationkineticsandgreenhousegasemissionsfromlegumecropresiduesaddedtosoilOliveiraM.1,RebacD.1,CoutinhoJ.2,TrindadeH.11-CentrefortheResearchandTechnologyofAgro-EnvironmentalandBiologicalSciences,UniversityofTrás-os-MontesandAltoDouro,(CITAB-UTAD);2-CentrodeQuímicadeVilaRealAfterharvestinglegumecropsforgrain,theremainingbiomassishighinNandCandcanincreasesoilfertilityforthefollowingcropwhenleftonthefield.However,thisprocessstimulatessoilmicrobialactivity,whichmayresultinthelossofCandNbynitrateleachingandbytheemissionof ammonia (NH3) and greenhouse gases (GHG) carbon dioxide,methaneandnitrousoxide(N2O).Thebio-chemicalcompositionoftheresiduesand themethodofapplication to thesoil can influence theNmineralizationrateandGHGemissions.An8-month laboratory soil incubation experiment is beingperformed.Treatments consist of three legume straws (cowpeas, fava beans andpeas)addedtosoilatafixedconcentrationof7,5t/habytwodifferentmethods: incorporation and surfacedeposition. Two temperatures (10and 20ºC) are being tested. Soil samples are collected periodically formineralNanalysis.Gaseousemissionsfromkilnerjarsweremeasuredinthefirst21dayswithanINNOVAphotoacousticfieldgasmonitor.Results for the first 21 days show significantly higher cumulative NH3emissions forpea and favabean residues left on surface than cowpearesidue, either incorporated or left on surface. Ammonia emissions at10ºCweresignificantlyhigherthanat20ºC.Incorporation of cowpea residue produced significantly highercumulativeN2Oemissionsinthefirst21daysthanallothertreatments.Further results from the on-going experiment are needed beforedrawingconclusions.

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P14–S2 FunctionaltraitsdiversityandfitnessevaluationinresponsetosaltstressinsomeMedicagosppwildpopulations.AmokraneM.S.Y.1,2,4,KadriA.1,2,3,LaouarM.1,BenC.4,JulierB.5,GentzbittelL.4,AbdelguerfiA.21-ENSA,Laboratoired’AméliorationIntegrativedesProductionsVégétales,Alger,Algérie2-ENSA,LaboratoiredesRessourcesGénétiquesetBiotechnologies,Alger,Algérie3-UniversitédeM’sila,Départementd’Agronomie,FacultédesSciences,BP166Ichebillia,M’sila,Algeria4-EcoLab,UniversitédeToulouse,CNRS,INPT,UPS,Toulouse,France5-URP3F,INRA,86600Lusignan,FranceLandsalinityisoneofthemajorabioticconstraintsthataffectnegativelycrop production worldwide. Medicago genus, mostly composed byannual species, is of a real interest in improving soil fertility andmaintainingpastures for livestock.Weassessedadaptive traits for salttolerancein40contrastedpopulationsbelongingto6Medicagospecies.In a common site, trial was performed in saline and non-saline soilswhere functional and fitness traits were recorded. Only M. ciliarispopulationssurvivedinthesalinesoil,highlightingthenegativeeffectofeven low salt levels at plant lift. ANOVA and PCA revealed that liftingrate, length branch at flowering time and total pod numbermight beinvolved insalt tolerance.TheresponsetosaltstressofMedicagowasfurther studied by analyzing germination kinetics of 37 populationsunder five ranges ofNaCl concentrations from0 to 200mM,during 7days. Kaplan-Meier survivor curves and Cox PHmodels indicated thattimeto50%germination,germinabilityandgerminationsynchronyareaffected by the increase of salts. The risk of non-germinated seedsdepends on species, populations and NaCl concentrations. Altogether,theseresultsshowthatbothsaltlevelandexpositiondurationaffectthephenotypicaltraits.Localadaptationmaypartlyexplainthepatternsofsalt tolerance.M. ciliaris is themost tolerant species,with a relativelyhighlevelofintraspecificvariabilityformoststudiedtraits.

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P15–S2 UMTALTER’N:Knowingalternativenitrogensources(legumesandorganicwasteproducts)inordertomanagecroppingsystemswithlownitrogenlossesandlessdependentonsyntheticfertilizersSchneiderA.1,ColnenneC.2,CadouxS.1,CellierP.3,DrouetJ.-L.3,HouotS.3,JeuffroyM.-H.2,LeGallC.1,ReauR.21-TerresInovia,DEO2-INRAAgronomie3-INRAEcosysLabelled by theMinistry of agriculture as an UMT (technologicalmixedunit) in2015,ALTER’Nisathematicpartnershipframeworkbetween Terres Inovia and INRA (Agronomie and Ecosys units inGrignon).ALTER’Naimstoenhancethecapacityforstrategicadvicefor inserting legumes (which fix atmospheric nitrogen) and forrecyclingresidualorganicfertilisingmattersintocropsystemsthatshould be both competitive and with low nitrogen losses. Theobjective is to produce references and to deliver knowledge andoperational tools. It should contributes that the actors ofagricultural and environmental world are able to make thediagnostic of possible nitrogen losses of their crops systems andthe conception and management of more N-efficients systems.Priority is given to knowledge synthesis by strengthening jointanalysis, so as to deliver validated items for systems, which usethese nitrogen sources as complements of the application ofindustrialmineralnitrogen.

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P16–S2 Howheatstressaffectsthephysiologyandreproductivebiologyofsummerandwinter-seasonfoodlegumecrops?NayyarH.1,GaurP.2,KumarS.3,SinghS.4,BindumadhavaH.5,NairR.M.6,PrasadP.V.V.7,SiddiqueK.H.M.81-DepartmentofBotany,PanjabUniversity,Chandigarh,160014India2-ICRISAT,Patancheru502324,GreaterHyderabad,Telangana,INDIA3-ICARDA,Rabat10112,Morocco 4-P.A.U.,Ludhiana141004,Punjab,India5-PlantPhysiology,WorldVegetableCenter,SouthAsiaHyderabad,India.6-WorldVegetableCenter,SouthAsia, ICRISATcampus,Patancheru502324GreaterHyderabad,Telangana,INDIA7-KansasStateUniversity,KansasManhattan,USA8 - TheUWA institute of Agriculture, TheUniversity ofWestern Australia, PerthWA6001,AustraliaHightemperature(heatstress)isamajorconcernfortheproductivityof food legumes, grown in winter as well as summer-season. Ourstudies have indicated a marked damage to reproductive stageresulting in reduction in pod set and seed yield of chickpea, lentil(cool-season legumes) andmungbean (warm-season legume) underhigh temperatures. Studies done in controlled and outdoorenvironments(latesowing)revealedthattemperatures>35/20°C(asdayandnight)werehighlydetrimental.Thedegreeofdamagevariesdepending upon the duration and severity of stress. Among thereproductivecomponents,pollengrainsweremoresensitive,becamedeformed and showed reduction in pollen viability, reducedgermination and pollen tube growth. Stigma receptivity and ovuleviabilitywerealsoinhibited,whichaffectedthepollengerminationonstigma surface and restricted tube growth through style, impairedfertilizationtocauseflowerabortion.Assessmentofthephysiologyofleavesandanthers indicateddecrease insucroseproduction inboththeorgansdueto inhibitionofenzymes,whichpossiblyaffectedthestructuralandfunctionalaspectsofthepollengrains.Oxidativestressincreased markedly in anthers due to heat stress, which possiblyaffected the pollen development and its function. Genetic variationfor stress tolerance exists in our target legume crops, which needsfurther probing and use of heat tolerant germplasm in breedingprograms.

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Session 3, parallel: Beneficial legume-microbeinteractions-RoomArrábidaIIIChaired by Carmen Bianco (Univ. Bari, Italy) and PedroFevereiro(ITQBNOVA,Portugal)14:30-15:00 Key lecture - Jens Stougaard: Receptormediatedsignalinginlegumesymbiosis

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OralCommunications15:00-15:10 Oral–S3Symbioticgenesexpressioninacontextofnitrogen-fixingsymbioticspecificityintheLupinusgenusKellerJ.1,Cabello-HurtadoF.1,SalmonA.1,ImperialJ.2,AïnoucheA.11 -UMRCNRS 6553 Ecobio,OSUR (Observatoire des Sciences de l'Univers de Rennes),UniversityofRennes1,263av.duGénéralLeclerc,35042Rennes,France2 -DepartamentodeBiotecnología (ETSIA)andCentrodeBiotecnologíayGenómicadePlantas(CBGP),UniversidadPolitécnicadeMadrid,28040Madrid,SpainAbilityoflegumestofixatmosphericnitrogenthankstotheirsymbiosiswithbacteriahasamajorrolefornaturalandagriculturalecosystems.Genesinvolvedinthedifferentstepsofsymbiosisformationhavebeenextensively studied in economically important or model species. Onestriking aspect of this legume-rhizobial mutualistic association is thesymbiotic specificity. In order to explore the complexity of thesymbioticspecificity,atranscriptomicstudyisconductedonagroupofnon-model species from genus Lupinus (Genistoids) usually formingnitrogen-fixing symbiosis with Bradyrhizobia. In addition, specificfeatures of symbiosis have been observed in this genus, such as: theabsence of infection threads; a uniquemode of bacteria penetration;and the occurrence of several cases of symbiotic specificity.Transcriptomes from root/nodules of three lupine species cultivatedwith twodifferent strainsof bacteriawere generated, assembledandannotated. Using alternative approaches to estimate the expressionlevel of either individual or clusters of transcripts, numerousdifferentiallyexpressed(DE)geneshavebeendetected.Carefulanalysisof these DE transcripts and clusters revealed the presence of manygenesinvolvedatdifferentstepsofthesymbiosisgeneticnetwork,suchasgenesinvolvedintheperceptionofsymbiontsignalsandinteractionwith NOD factor receptors. This provides new insights on geneexpressioninacontextofsymbioticspecificity.

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15:10-15:20 Oral–S3BeneficialmicrobesassociatedwithlegumesVosatkaM.1,RozmosM.1,LatrA.1,MaY.21-SymbiomLtd2-UniversityofCoimbra,PortugalSymbiotic rhizobia, plant growth promoting rhizobacteria (PGPR) andarbuscularmycorrhizalfungi(AMF)arecommonassociatesofmajorityof legumes. The trials undertaken within Eurolegume project bySYMBIOM Ltd. (producer of AMF inocula) confirmed the mutualcompatibility between the microbial inoculants (rhizobia, PGPR andAMF)inpeaandfababeanplantsintermsoftheirestablishmentintherootsaswellastheimpactofdroughtstressonplantdevelopmentandyieldparameters.Interestingly,therewasnodifferencefoundbetweenthe irrigation regimes in terms of dry biomass of faba bean. Positiveeffectswereobservedregardingseedweightperplantoffababean.Inaddition, the leaf analysis showed an increase of the content ofphosphorus in allmicrobial treatments for pea under 100% irrigationregime. Suitable combination of rhizobia and AMF for these legumespluschickpeaandcowpeahavebeentestedandtheireffectsongrowthand stress resistance evaluated. Feasible application modes aredeveloped for microbe delivery to cultivation. Potential of largescaleapplicationofinoculainlegumeproductionisconsidered.Project has received funding from the European Union´s SeventhFramework Programme for research, technological development anddemonstrationundergrantagreementno613781.Providingoflegumeseeds and rhizobia by Dr. Rui Oliveira from University of Coimbra isacknowledgedaswellascollaborationwithotherEurolegumepartners.

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15:20-15:30 Oral–S3PartnerchoiceinacorecollectionofpeainoculatedbyamixoffiveRhizobiumleguminosarumsbv.viciaestrainsBourionV.,deLarambergueH.,AubertV.,Chabert-MartinelloM.,SiolM.,DucG.,BurstinJ.INRA,21000Dijon,FancePea is the third most important grain legume worldwide. The cropnatural ability to use, asmain nitrogen resource, the atmosphericN2symbioticallyassimilatedbyRhizobiumleguminosarumsbv.viciae(Rlv)in the plant nodules is a major component of its attractiveness.However, the symbiosis may not be optimal because natural Rlvpopulations are quantitatively and qualitatively heterogeneous, withstrains varying in competitiveness and efficiency of nitrogen fixation.The variability of pea-Rlv partner choice was investigated within acollectionof104peaaccessionsco-inoculatedwithamixoffivediverseRlv strains. Analyses of the genetic structure of the pea collection -genotyped using the GenoPea 13K SNP Array - uncovered differentgeneticgroupsrepresentativeofthepeageographicoriginorhistoryofselection. Proportions of nodules formed with each strain wereestimated in each pea accession. Differences in the Rlv choice wereobservedbetweenthedifferentpeageneticgroups,revealingchangesin partner choice during domestication and breeding selection.Additional experiments performed on a subset of pea accessionsshowed that in most cases competiveness for nodulation of a givenpea-Rlv symbiotic association could not be related to its nitrogenfixationefficiency.FurtherstudiesinvolvinglargerpeapanelandmixofRlvstrainstogetherwithahigherdensitySNPgenotypingwillbecarriedouttoidentifyspecificlociunderlyingthepartnerchoicetrait.

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15:30-15:40 Oral–S3Improvingadaptationoflegume-rhizobiumsymbiosistothesoilenvironmentLepetitM.,BrunelB.,HeulinK.,LahmidiN.A.,LeQuéréA.,PerventM.,TauzinM.LaboratoiredesSymbiosesTropicalesetMéditerranéennes,UMR1342INRA-IRD-CIRAD-UM-Montpellier SupAgro, Campus International de Baillarguet TA-A82/J, 34398MontpellierAmajorhallmarkoflegumecropsisadaptationtomineralNlimitationbydevelopingrootsymbioticnoduleswithN2-fixingrhizobia.Theirusemay reduce nitrogen fertilizer inputs. However nodules are highlysensitive to soil constraints. Soils do not necessarily shelter symbioticpartners foroptimalN2 fixation.Localwaterdeficitandotherstressessuppress rapidly the activity of nodules directly exposed to them insoils. Consequently yields of symbiotic legumes are frequently highlyfluctuating.Thereisaneedforbreedingstrategies.A first strategy is to select rhizobium/legumeassociations thatdisplaythe most efficient N2 fixation and allow success for rhizobiuminoculation at sowing. Bacteria forming nodules are not necessarilyefficient and may display different levels of competitiveness fornodulationwhentheyareinmixture,asitisgenerallytheruleinsoils.The second strategy is to identify genetic determinants that allowplantstocompensateapartialsuppressionofitssymbioticactivitydueto a localized soil constraint (notably drought). Several systemicmechanisms adjusting the symbiotic capacity to the plant N demandare involved. Compensatory responses may vary according to thesymbioticpartner.Recentprogress toward these twoobjectivesusingMedicago truncatula/Sinorhizobium as model system andpea/Rhizobiumleguminosarumasagronomicaltargetwillbepresented.Thiswork has been supportedby Peamust (ANR) and Legato (EU FP7KBBE)grants.

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PostersP17–S3

Seedcoatingofgrainlegumeswithinoculaofarbuscularmycorrhizalfungiandplantgrowth-promotingbacteria.PintoL.M.1,CambraR.1,RochaI.2,MaY.2,MarquesG.3,LátrA.4,VosátkaM.5,FreitasH.2,OliveiraR.S.1,21-DepartmentofEnvironmentalHealth,ResearchCentreonHealthandEnvironment,SchoolofAlliedHealthSciences,PolytechnicInstituteofPorto,VilaNovadeGaia,Portugal.2-CentreforFunctionalEcology,DepartmentofLifeSciences,UniversityofCoimbra,Coimbra,Portugal.3-CITAB-UniversityofTrás-os-MontesandAltoDouro(UTAD),DepartmentofAgronomy,VilaReal,Portugal.4-SymbiomLtd.,Lanskroun,CzechRepublic.5InstituteofBotany,AcademyofSciencesoftheCzechRepublic,Pr_honice,CzechRepublic.Beneficialsoilmicroorganismssuchasplantgrowth-promotingbacteria(PGPB) and arbuscular mycorrhizal fungi (AMF) can improve plantgrowth,nutritionandyield.Seedcoatingtechniqueshavethepotentialto allow the use of minor amounts of inoculum, resulting in costreductionandefficiency increase.Theaimof thisstudywas toassesswhether seed coating can be used as a feasible microbial inoculadelivery system for grain legumes. Seedsof chickpea (Cicer arietinumL.) and cowpea [Vigna unguiculata (L.) Walp.] were coated withinoculum of PGPB, AMF or amixture of both. Seed germination rateandthenumberofviablePGPBandthenumberofpropagulesofAMFper seed of chickpea and cowpea were determined. Results showedthat both microbial inoculants were viable after being coated ontoseed of chickpea and cowpea. Seed coating may represent asustainable approach for application of beneficial microorganisms intheproductionofgrainlegumes.Acknowledgements:R.S.Oliveira,Y.MaandI.Rochaacknowledgethesupport of Fundaçãopara a Ciência e a Tecnologia (FCT) through theresearch grants SFRH/BPD/85008/2012, SFRH/BPD/ 76028/2011 andSFRH/BD/100484/2014andFundoSocialEuropeu(FSE).ThisworkwasfinancedbyPortuguesenational fundsthroughProgramaOperacionalCompetitividade e Internacionalização (POCI) and Fundo Europeu deDesenvolvimentoRegional(FEDER)underProjectPOCI-01-0145-FEDER-016801andbyFCTunderProjectPTDC/AGR-TEC/1140/2014.

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P182–S3 FindingofsuperiorRhizobiumstrainsforfieldpeaandfababeaninEstoniansoilsToomM.1,NaritsL.1,SooväliP.1,PõllumaaL.1,MäeA.11-EstonianCropResearchInstituteTheincreasedglobalNfixationfromlegumesrequirestheselectionanddevelopmentofeffectiveinoculantstrainsofroot-nodulebacteriathatsuitwithlegumehostandthetargetedaphicenvironment.Within the framework of the project EUROLEGUME (Enchancing oflegumes growing in Europe through sustainable cropping for proteinsupply for food and feed) the trials with selecting appropriateRhizobiumstrainswerecarriedout.TherootnodulesofFababean(Viciafaba)‘Jõgeva’andfieldpea(Pisumsativum)‘Mehis’werecollectedfrompotgrownplantscontainingsoilsfromdifferentEstonianregions.StrainsofRhizobiumwere isolatedbystandard methods. The composition of bacterial community weredeterminedby16SrRNAsequencing.The effect of Rhizobial strains onnodulation and growthof bean andpea was examined in test tubes with seedling agar. Also the potexperiment with inoculated and uninoculated soil was carried out toevaluate the nodulation efficiency, plant growth, seed yield, nitrogenand protein content. Further, the acetylene reduction assay will beappliedtoestimatebiologicalN2fixationactivity.Acknowledgements:TheprojectEUROLEGUME,Grantno613781

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P183–S3 Phenotypicandmolecularidentificationofrhizobianodulatingfababean(ViciafabaL.)andcowpea(Vignaunguiculata(L.)Walp)plantsPereiraS.1,MuchaA.1,SharmaL:1,PereiraG.2,DuarteI.2,RosaE.1,MarquesG.11 - CITAB- University of Trás-os-Montes and Alto Douro (UTAD), Department ofAgronomy,VilaReal,Portugal.2-InstitutoNacionaldeInvestigaçãoAgráriaeVeterinária(INIAV),Elvas,Portugal.Within the diversity of leguminous plants, cowpea and faba bean arevery important in Europe, and in Portugal. In this work, 37 rhizobiaisolatesofcowpeaand27rhizobiaisolatesoffababeanwerecollectedin different geographic regions and edaphoclimatic conditions. Thephenotypiccharacterizationof the isolateswasperformedaccordinglyto their colony characteristics, Molecular identification of theseisolates,aftercheckingtheirabilitytocolonizeotherplantsinvitro,wasperformed by 16S rDNA sequences. Other methods such as genomicfingerprinting using the repetitive sequence-based polymerase chainreaction (repPCR)method, with REP, ERIC and BOX primers and nifHandnodCsequencesfromselectedisolateswereusedwhennecessaryto increase the discriminating power. Although the genusBradyrhizobium ismorecommonincowpea,inthisworkwehavealsoisolated strains of Rhizobium that do not cluster with the existingspecies that have been identified. In the bacteria collected from fababeannodules,someisolatesbelongingtothegenusBurkholderiawereidentified.Acknowledgement: Authors gratefully acknowledge the funding from7th Research Framework Programme of the European Union forfinanciallysupportingthisresearchthroughtheproject“EUROLEGUME:Enhancingof legumesgrowinginEuropethroughsustainablecroppingforproteinsupplyforfoodandfeed”.

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P184–S3 YieldformationofFababeans(ViciafabaL.)dependingondoubleinoculationwithrhizobiaandmycorrhizafungiSenbergaA.,DubovaL.,AlsinaI.LatviaUniversityofAgricultureLegumesareabletofixthenecessaryamountofatmosphericnitrogenbecause of symbiotic relationship with soil bacteria rhizobia. Whilerhizobiaensurelegumeswithnitrogen,theuptakeofmineralelements(particularly phosphorus) can be enhanced by mycorrhiza fungi.Legume inoculation with mycorrhiza increases protein accumulationandalsotheactivityofnodules.Aimof the studywas toexamine theeffectivenessof triple symbiosisand to test if double inoculationof fababeanswith rhizobia togetherwithmycorrhizacanenhancethequantityandqualityofthecrop.Fourdifferent faba bean cultivars were used (Bartek, Karmazyn, Fuego,Lielplatone). Rhizobia strains (RP023, RV407) were provided by theInstitute of Soil and Plant Sciences (Latvia). Commercial mycorrhizapreparationwasobtainedfromSymbiomLtd.(CzechRepublic).Freshweight,heightandthelevelofbeanroot infectionwithrhizobiaand mycorrhiza were detected during the flowering phase. Afterharvesting,thecropyieldandproteincontentofbeansweremeasured.Obtained data shows that crop yield and protein content depend onbeancultivar,microorganisminoculantandontheweatherconditionsduring the vegetation period. Bean inoculation with rhizobium strainRV407,whichisoriginallyisolatedfrombeans,wasthemosteffective.Treatment with mycorrhiza showed a positive impact on yieldformation.Thehighestproteincontentwasdetected inthefababeancultivarLielplatone.

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P185–S3 Intercroppinglegume-cerealsisasystemtovaluelegume-rhizobiasymbiosistowardagriculturesustainabilityMouradiM.1,MakoudiB.1,KabbadjA.1,FarissiM.2,BouizgarenA.3andGhoulamC.11 - Unit of Plant biotechnology and Symbiosis Agrophysiology, Faculty of Sciences andTechniques,PObox549,Gueliz,Marrakech,Morocco;2 - Université SultanMoulay Slimane, Faculté Polydsciplinaire, B. P. 592, Mghila, BeniMellal,Morocco;3 - Institut National de la Recherche Agronomique (INRA), Unité d’AméliorationGénétiquedesPlantes,B.P.533,Guéliz,Marrakech,MarrocoIntercropping legume-cereal is one of themain farming systems thatoptimize use of common limiting resources.Our study aims to assessthebenefitof fababean-rhizobia symbiosisonbarleyplantgrowthasintercropped species particularly under soil nitrogen (N) andphosphorus(P)deficiency.Thestudywascarriedout infarmers‘fieldsusing two Vicia faba varieties and one barley variety grown asintercropped species in N and P deficient soils in the Haouz area ofMorocco.Weconsideredsolecropoffababean;solecropofbarleyandintercroppedfababean-barleyasalternaterowswithplantdensitiesof30 and 50 plants per m2 respectively for faba bean and barley. Thetrialswereconductedwithoutchemicalfertilization.Atfababeanplantfloweringstage,thetrialswereassessed.Theresultsshowedthatfababean plant growth and nodulation were positively correlated tophosphorus accumulation in different plant parts, whereas thephosphatases activities in nodules and roots were higher inintercropped faba beans than sole crop. For barley, plant biomasssignificantly increasedwhengrownasmixedculturewithfababeanincomparisontothecorrespondingsolecropcontrols.Indeed,thisstudyproved that intercropping system stimulates the activity ofphosphatases and increases P availability in the rhizosphere of bothspecies.Thereby,inintercroppingsystem,fababean-rhizobiasymbiosisimprovedbarleyplantgrowththroughincreaseofnutrientsavailability.

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P186–S3 Chickpeaandlentilco-inoculationwithselectedplantgrowthpromotingrhizobacteriaandrhizobia:impactonnodulationandgrowthBenjellounI.1,2,Thami-AlamiI.2,ElKhadirM.2,UdupaS.M.3,AuragJ.4,BerrahoE.4,DouiraA.11-LaboratoiredeBotanique,BiotechnologieetdeProtectiondesPlantes,Départementde Biologie, Faculté des Sciences de Kenitra, Université Ibn Tofayl, BP 242, Kénitra,Morocco.i.benjelloun@hotmail.fr2-InstitutNationaledelaRechercheAgronomique(INRA),B.P.415,Rabat,Morocco3 - ICARDA-INRA Cooperative Research Project, International Center for AgriculturalResearchintheDryAreas(ICARDA),B.P.6299,Rabat,Morocco4 - Laboratoire de microbiologie et de biologie moléculaire (LMBM) UniversitéMohammedVFacultéDesSciences,RabatAgdalFood legumes play a substantial socio-economic role and occupy animportantplaceinthedietofMoroccanpopulation.However,theyieldremainslow.Chickpea(Cicerarietenum)andlentil(Lensculinaris)areinthe second rank inMorocco, after faba beanwith a strong local andinternational demand. Culture and productivity of these two crops inMoroccodependslargelyonthechemicalfertilization.Toenhancetheproductivityand limit theuseof chemical fertilizers, it isnecessary tosupport theuseofbiofertilizersbasedonnitrogen fixingbacteria andphosphate solubilization Rhizobacteria for an economic andecologically-sustainableagriculture.A field experiment co-inoculation test of lentil and chickpea withrhizobia(L3andL43forthelensandPc72andPc100forchickpea)andplant growth promoting bacteria (M131 and P1S6)was carried out intwositesinMorocco,Merchouch(clay-silty)andAinSbit(clay-sandy)inaRandomizedCompleteBlockDesign (RCBD)with four replicationsofeach treatments. 16 treatments were applied in each site to eachculture: twoRhizobia strainsS1andS2, twoPGPRstrains (M131andP1S6), twoN levels (N0andN120)andtwophosphate levels (P0andP80).Results showed that inoculation with rhizobia P72 and P100 forchickpea and L3 and L43 for lentil improved significantly grain andstrawyieldofbothculturesinbothsites.

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P187–S3SynergybetweencropdiversityandearthwormcommunityimprovecropyieldsDrutB.,CassagneN.,CannavacciuoloM,FustecJ.URLEVA,EcoleSupérieured’Agricultures,Angers,FranceBiodiversity may influence productivity of the plant cover throughabove- and belowground interactions. Our study aimed to analyzesimultaneous effects of plant cover diversity and of earthworms onwheatperformances.Mesocosmsfilledwithsoilweresownwitheither6wheat plants of the same cultivar, or 6 plants of 3 differentwheatcultivars, or 3 wheats of 3 different cultivars with 3 clover plants (T.hybridum). They were inoculated or not with earthworms (n=5): i) 5endogeic earthworms, ii) 5 endogeic and2 anecic earthworms, iii) noearthwoms. Above-and belowground plant biomass and Nconcentrations were measured. The Relative Interaction Index wascalculatedtohighlightthestrengthofcompetitionbetweenplants.Thecultivarmixturehadnoinfluenceonthewheatperformance,butwithclover, competitiondecreased to thebenefitofwheatbiomassandNaccumulation. Earthworms also decreased competitive strengthbetween wheats in mixed cultivars as well as in intercrop. In thepresence of clover, wheat biomass and N were increased thanks tonichecomplementarity.Endogeicearthwormschangedthe interactionbetween plants and highly reduced belowground competition,suggesting that plant cover diversity and earthworms worksynergistically to improve wheat yields. The addition of earthwormfunctional diversity had no effect onwheat intercroppedwith clover,whichsuggestthatthebenefitfrominteractionsreachedathreshold.

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P188–S3 Theuseofnitrogenfixingbacteriaas“eliteinoculants”inbiodiverselegumepasturesSoaresR.1,FareleiraP.1,PereiraP.1,SilvaA.R.2,BarradasA.2,CrespoD.2,VideiraeCastroI.11-INIAV,I.P.,Oeiras,Portugal2-Fertiprado,Vaiamonte,PortugalThe improvementofpastures in themontado (corkoak)ecosystem isbased on the establishment of biodiverse permanent pastures rich inlegumes, by sowing a diversity of selected and improved species, inwhich inoculated legumes with rhizobia are preponderant. This isconsideredasapowerfulmanagement tool,being legumes importantcomponents of the strategy for increasing productivity andsustainability, using symbiotic nitrogen fixation as amajor process ofproviding nitrogen to the soils. Nodulation and N2 fixation in thesesymbioses need that host andmicroorganisms arewellmatched. Theselectionfortheoptimalcombinationofrhizobiaandthehost legumeusuallyresultsinmoreeffectivesymbioses.Despitethelargenumbersof rhizobial strains that nodulate host legumes, only some strains aresymbioticallyeffectiveontheirhosts.Theaimofthisworkwastoselectautochthonous rhizobia strains isolated fromseveralTrifolium sp. andMedicagosp.grownintheSouthofPortugal,towardstheirfutureuseas elite inoculants. Experiments in controlled environment conditionswere performed to evaluate the symbiotic effectiveness of differentspecies of Trifolium and Medicago inoculated with several rhizobiastrains.Molecular identificationwasperformedusing thesequenceof16S rRNA and recA genes. The performance and competitiveness ofselectedstrainswereevaluatedinnaturalfieldconditions.ThisworkwassupportedbytheprojectPRODER54971.

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P189–S3 Theauxinindole-3-aceticacid(IAA)ismorethanaplanthormoneBiancoC.1,AndreozziA.1,RubinoS.1,EspositoR.2,AngeliniC.3,DefezR.11 - Institute of Biosciences and BioResources, CNR, via Pietro Castellino 111, 80131Napoli,Italy2-InstituteforAppliedMathematicsMauroPiconeIAC,CNR,viaP.Castellino111,80131Napoli,Italy3-InstituteofGeneticsandBiophysics“A.B.T.”,CNR,viaP.Castellino111,80131Napoli,ItalyRNA-seqanalysiswasappliedtocompare,underfree-livingconditions,thetranscriptomeoftheIAAoverproducingS.melilotiRD64strainwiththewild typeS.meliloti1021one.Quantitative real-timePCRanalysiswas used to validate RNA-seq experiments and to evaluate, undermicroaerobic andnitrogen-limited conditions, theexpression levels ofgenesdirectlyinvolvedinnitrogenfixationandmetabolism.OurresultsshowedthatIAAoverproductionbyrhizobiadirectlyorindirectlyleadstotheactivationofkeygenesofnitrogenfixationprocessincludingthemain nitrogen fixation regulator fixJ, the two intermediate regulatorsfixKandnifA,andseveralothergenes,knowntobeFixJtargets,whoseexpression normally occur in nitrogen fixing root nodule. The genecoding for the sigma factorRpoH1andother genes involved in stressresponse, regulated in RpoH1-dependent manner in S. meliloti, werealso induced inRD64cells.Undermicroaerobiccondition,quantitativereal-timePCRanalysis revealedthat thegenes fixJLandnifAwereup-regulated inRD64 cells as compared to1021 cells.We speculate thatIAA could be a plant signal used to induce the expression of rhizobianitrogen fixation genes inside root nodules.We also hypothesize thatthebacterialIAA-overexpressionmightimprovethenitrogenfixationindifferent plant-bacteria associations that could poorly establish amicroaerobicenvironment.

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P190–S3 Symbioticgenesexpressioninacontextofnitrogen-fixingsymbioticspecificityintheLupinusgenusKellerJ.1,Cabello-Hurtado1F.,SalmonA.1,ImperialJ.2,AïnoucheA.11 -UMRCNRS 6553 Ecobio,OSUR (Observatoire des Sciences de l'Univers de Rennes),UniversityofRennes1,263av.duGénéralLeclerc,35042Rennes,France2 -DepartamentodeBiotecnología (ETSIA)andCentrodeBiotecnologíayGenómicadePlantas(CBGP),UniversidadPolitécnicadeMadrid,28040Madrid,SpainAbilityoflegumestofixatmosphericnitrogenthankstotheirsymbiosiswithbacteriahasamajorrolefornaturalandagriculturalecosystems.Genesinvolvedinthedifferentstepsofsymbiosisformationhavebeenextensively studied in economically important or model species. Onestriking aspect of this legume-rhizobial mutualistic association is thesymbiotic specificity. In order to explore the complexity of thesymbioticspecificity,atranscriptomicstudyisconductedonagroupofnon-model species from genus Lupinus (Genistoids) usually formingnitrogen-fixing symbiosis with Bradyrhizobia. In addition, specificfeatures of symbiosis have been observed in this genus, such as: theabsence of infection threads; a uniquemode of bacteria penetration;and the occurrence of several cases of symbiotic specificity.Transcriptomes from root/nodules of three lupine species cultivatedwith twodifferent strainsof bacteriawere generated, assembledandannotated. Using alternative approaches to estimate the expressionlevel of either individual or clusters of transcripts, numerousdifferentiallyexpressed(DE)geneshavebeendetected.Carefulanalysisof these DE transcripts and clusters revealed the presence of manygenesinvolvedatdifferentstepsofthesymbiosisgeneticnetwork,suchasgenesinvolvedintheperceptionofsymbiontsignalsandinteractionwith NOD factor receptors. This provides new insights on geneexpressioninacontextofsymbioticspecificity.

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Session 4, parallel: Genetic resources - RoomArrábidaIVChaired by Hari Upadhyaya (ICRISAT) and Rodomiro Ortiz(SLU,Sweden)14:30-15:00Keylecture-NoelEllis:Whereareweafter150yearsoflegumegenetics?

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OralCommunications15:00-15:10Oral–S4Usingexpandedcollectionsofwildrelativesofchickpeatounderstanddomesticationandimproveclimateresilience.vonWettbergE.1,ChangP.2,SinghV.2,CordeiroM.2,GreenspanA.3,AlfordB.4,CarrasquillaN.3,Dacosta-CalheirosE.1,WarschefskyE.1,RoufMirR.5,BukunB.6,KahramanA.7,AydoğanA.8,BergerJ.D.9,NuzhdinS.V.2,RVarmaPenmetsaR.V.10,CookD.R.101-FloridaInternationalUniversity,Miami,FL,2-UniversityofSouthernCalifornia,LosAngeles,CA,3-UniversityofCalifornia,Davis,CA,4-UniversityofCaliforniaDavis,Davis,CA,5-SKUASTJAMMU,JAMMU,India,6-DicleUniversity,Diyarbakir,Turkey,7-HarranUniversity,Urfa,AZ,Turkey,8-TurkishMinistryofAgriculture,Ankara,Turkey,9-PlantIndustry,CSIRO,Floreat,WA,Australia,10-UniversityofCaliforniaatDavis,Davis,CALikemanyother annual crops, chickpea (Cicer arietinum) underwent asubstantial population bottleneck when domesticated from its wildprogenitor (Cicer reticulatum) approximately 10 thousand years ago.During this bottleneck several key domestication traits such asindehiscencewere positively selected by early farmers. However, thesmall effective population sizes of early agriculture and the ecologicaltransition to the protected habitat of a cultivated field likely relaxedselection on much of the cultivated chickpea genome. We documentbothpositiveandrelaxedselectiononthecultivatedchickpeagenome,utilizingarecentlyassembledexpandedcollectionofthewildprogenitorof chickpeaaswell as its sister taxaCicer echinospermum.A thoroughsurveyof the sourcepopulations for thewild relatives aswell as theirhabitatsallows inference into the levelsof standingvariationavailabletoearlyfarmers,andenvironmentalfactorsthatshiftedwiththeadventofagriculture in the fertilecrescent. Ourworkshows theneed for in-depth collections of crop wild relatives and landraces shielded frommodernbreedingtomakeinformedinferencesaboutdomestication.

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15:10-15:20 Oral–S4Characterizingthegeneticdiversityofcowpeaaccessionsusingahigh-densitySNParrayCarvalhoM.1,CastroI.1,2,MatosM.2,3,Lino-NetoT.4,CloseT.5,Munoz-AmatriainM.5andCarnideV.1,21 - Centre for Research and TechnologyofAgro-Environment andBiological Sciences(CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real,Portugal.2-DepartmentofGeneticsandBiotechnology,UniversityofTrás-os-MontesandAltoDouro(UTAD),5000-801VilaReal,Portugal.3 -Biosystems& IntegrativeSciences Institute (BioISI), SciencesFaculty,UniversityofLisbon,CampoGrande,1749-016Lisbon,Portugal.4-Biosystems&IntegrativeSciencesInstitute(BioISI),PlantFunctionalBiologyCenter(CBFP),UniversityofMinho,CampusdeGualtar,4710-057Braga,Portugal.5 - Department of Botany and Plant Sciences, University of California Riverside,Riverside,California92521-0124USA.Cowpea, native toAfrica, is a legume cropwell adapted to hot anddrought-prone environments, and a primary source of protein formillionsofpeopleinthedevelopingworld.Characterizationofgeneticdiversitywithincowpeagermplasmis importantforthepreservationofgeneticresourcesandtheiruseincropimprovement.WeusedtheIlluminaCowpeaiSelectConsortiumArraycontaining51,128SNPstogenotype 96 cowpea accessions including: 33 local landraces andcultivarsfromPortugal,and63landracescollectedworldwide(atotalof 24 countries). A total of 44,056 high-quality SNPs werepolymorphic among the samples and used for genetic diversityanalyses. Both STRUCTURE and principal component analysisidentified four subpopulations, mainly differentiated by accessionsgeographical origin. Most Portuguese accessions were clusteredtogether with those from other southern European and northernAfricancountries,indicatinggeneticsimilarityamongthem.However,two Portuguese cowpeas did not belong to this subpopulation andinsteadwere categorized as ‘admixed’ (1 accession) or belonged toanothersubpopulation(1accession).Theseaccessionscouldbeusedassourcesofdiversityinplantbreedingprograms.Acknowledgments: This study was supported by EUROLEGUMEproject.ThisprojecthasreceivedfundingfromtheEuropeanUnion’sSeventh Framework Programme for research, technologicaldevelopmentanddemonstrationundergrantagreementno613781.

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15:20-15:30 Oral–S4LupiBreed-Valorisationofnovelgeneticvariabilityinnarrow-leafedlupinFischerK.1,RouxS.1,JansenG.2,UlrichH-J.2,DieterichR.3,WehlingP.1,Ruge-WehlingB.11 - Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute forBreedingResearchonAgriculturalCrops,GroßLüsewitz,Germany,2 - Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute forResistanceResearchandStressTolerance,GroßLüsewitz,Germany,3-SaatzuchtSteinachGmbH&Co.KG,Bocksee,GermanyNarrow-leafed lupin provides a source of protein and fibre withexceptionalfunctionalproperties,whichmaybeusedforavarietyofpurposesincludingtheproductionofhigh-qualityvegetablefoods.Tokeep lupingrowingeconomicallyattractivetothefarmer, furtherimprovement of grain and protein yield is mandatory. This may beaccomplished by plant breeding. However, genetic variability ofadvanced breeding materials of narrow-leafed lupin is limited. Togenerate novel genetic variants we started an EMS-basedmutagenesis program using current varieties. Novel growth typeswith potential for higher yield were selected and propagated tophenotypically stable lines. Performance tests over three years andthree replications were started in 2015 to record agronomicalrelevant traits suchas grain yield, protein yield, pod shattering, andanthracnose resistance. Statistical analysis of field data (2015)revealed M lines with higher yield and higher protein contentscomparedtotheinitial‘wildtype’.Amongthese,someMlinesthosealso expressed reduced pod shattering and/or reduced anthracnoseinfestation.TheselectedMlinesarecurrentlycrossedwithgenotypescarryingdefinedanthracnoseresistancegenes.Thisbreedingprogramisassistedbymolecularmarkersandexpectedtoresultinnovel,high-performingcultivars.

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15:30-15:40 Oral–S4Domesticbliss?Causesandconsequencesofamoderneradomesticationeventinnarrow-leafedlupinNelsonM.1,2,MousaviM.2,TaylorC.2,BayerP.2,KamphuisL.3,BergerJ.3,ClementsJ.4,HaneJ.5,EdwardsD.2,ErskineW.2,CowlingW.21-RoyalBotanicGardensKew,2-UWA(UniversityofWesternAustralia),3-CSIRO(CommonwealthScientificandIndustrialResearchOrganisation),4 - DAFWA (Department of Agriculture and Food of Wetern Australia), 5 CurtinUniversity,AustraliaThe tamingofwildplants tobecomeproductive agricultural cropswas a gradual process taking hundreds or thousands of years formost of our major crop species. It involved progressivelyaccumulating domestication traits (such as removal of seeddormancy and reducing seed dehiscence) that made the plantsincreasinglymore useful and productive to people. Narrow-leafedlupin (Lupinus angustifolius L.) is exceptional in that itsdomestication was both recent and rapid, starting humbly as agreen manure and fodder crop in the Baltic States in the 19thCenturyandthenrapidlybeingtransformedintoahigh-qualitygraincrop in the 20th Century. Thiswas achieved through fixation of ahandful of domestication alleles: reduced seed dehiscence (lentusand tardus), removal of seed dormancy (mollis), altered time offlowering and maturity (Ku), and reduced seed alkaloid content(iucundus). Both ancient and modern domesticates share this iscommon: seriously depleted genetic diversity due populationbottlenecks.Narrow-leafedlupin,asa20thCenturydomesticate,isunique among legume grain crops in that these bottleneck eventsarealldocumentedinthescientificliterature.Herewewilloutlinethevalueoflupinasamodelforunderstandingplant domestication, what is known about the molecularmechanismsofdomesticationtraits,andthe impactdomesticationhad on genome-wide diversity in this modern era legume cropspecies.

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PostersP18–S4 GeneticresourcesoffoodlegumecropsinAlgeriaGaadD.NationalHighSchoolofAgriculture,AlgeriaFoodlegumegeneticresourcesplayaveryimportantanddiverseroleinfood security, farming systems and poverty alleviation, particularly indeveloping countries. They are ideal crops for achieving food andnutritional security forpoorconsumers. Food legumesalso serveasafeed crop in many farming systems. In Algeria, Lentil (Lens culinaris),Chickpea(Cicerarietinum),Pea(Pisumsativum),Fababean(Viciafaba),andcommonbean(Phaseolusvulgaris)arethemainfoodlegumecropsgrown under rainfed condition. Thiers area is estimated at 10000 HA.The paper discusses the characteristics of the main food legumecultivatedinAlgeria,themajorissuesrelatedtotheirgeneticresourcesmaintenance,useandimportanceoftheirbiodiversity.

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P19–S4 EvaluationoffababeansgeneticresourcesforfoodandfeedLepseL.,KronbergaA.,OlleM.,KokareA.,KonošonokaII.,PereiraM.G.,BebeliP.PūreHorticulturalResearchCentre,LatviaFaba beans (Vicia faba L.) become more and more popular due tonutritional value as protein source for food and feed and due to itsagricultural service crop properties – biological nitrogen fixation, soilbiologicalactivityincreasingandbreakcropinmonoculturesystems.During the first two years of trials (2014 and 2015) genotypes andlandracesofEuropeanoriginoffababeanwhereinvestigatedintermsof their genetic diversity and productivity parameters. Samples oflocally grown commercial varieties and old cultivarswhere comparedwith genetic resources accessions from gene banks and collected incollectionmissions.Theyieldparametersrevealedthatthenumberofpodspernodevariedbetween0.9and2.6inaverage.Thehighestnumberofpodspernodewere found in the cultivars `Favel`, `Solberga`, `Gerd`, Fb-2939, and`Gubbestad`forbroadbeansandin`Bauska`,`Lövånger`,`Lielplatones`,`Valmiera`,VF_013and`Gloria`forfieldbeans.Thegenotypeswiththehighest seed weight per plant were `VF_001`, `VF_003`, `VF_004`,`VF_005`,`VF_009`and`Gerd`inbroadbeansandfor`Prieku_u-32`and`Lielplatones` in field beans. Overall, it was found that the mostpromisingbroadbeancultivaraccessionsare`VF_001``VF_54`andfieldbeans`Prieku_u-32``Gubbestad`,`Lielplatones`.The research was funded by the European Union’s 7th FrameworkProgramme for research, technological development anddemonstrationundergrantagreementNo613781,EUROLEGUME.

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P20–S4 Morphologicalandphysiologicalcharacterizationof15accessionsofcowpeaFernándezJ.A.,Martos-FuentesM.,WeissJ.,Egea-GilabertC.,Egea-CortinesM.UniversidadPolitécnicadeCartagena.InstitutodeBiotecnologíaVegetalCowpea (Vigna unguiculata) is a South African crop, used both forhuman and animal consumption. The aim of this work was themorphological and physiological characterization of 15 accessions ofcowpeaforfurtherselectioninabreedingprogram.Weanalysed6localaccessionsofcowpeafromSpain,6fromPortugal,2fromGreeceandareferencebreedinglinefromUSA(IT97K-499-35).Descriptorswereusedfor the characterization according to the International Board for PlantGenetic Resources. From all descriptors analysed, themost importantwas seed protein content,which ranged between 17% and 26%. Seedweightperplantrangedbetween5gand40g,andthecultivarwiththelargestpodreached65cmmorethantheshortest.Therefore,significantdifferencesbetweencultivarswerefoundforseedweightperplant,podlengthandseedproteincontent,indicatingthatthegeneticvariabilityofcultivarsplaysanimportantroleinplantperformance.

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P21–S4 EvaluationofpeageneticresourcesforfoodandfeedKronbergaA.,OlleM.,PereiraM.G.,KokareA.,KonošonokaI.,LepseL.InstituteofAgriculturalResourcesendEconomics,LatviaAs cultivation history of pea is very long, a broad gene pool isdeveloped during centuries. However, a tremendous amount ofgeneticresourcesisnotevaluateduntilnowforitspotentialbenefitsinregardtobiochemicalandmorphologicalfeatures.During the first two years of trials (2014 and 2015) genotypes andlandraces of European origin of peawhere investigated in terms oftheir phenotypic diversity. Samples of locally grown commercialvarieties and old cultivars where compared with genetic resourcesaccessionsfromgenebanksandcollectedincollectionmissions.Field trials were established in Latvia, Estonia and Portugal. Broadvariation was stated between locations and traits for genotypesincluded in evaluation. Soplant length variedbetween16.8 cmand186cm.Thenumberofdaystoreachthefullripeningrangedfrom89and110days.100seedsweightshowed largevariancerangingfrom7.9gto38.1g.Averageseedweightperplantvariedbetween0.5gplant-1 and 15.5 g plant-1. Protein content in dry pea grains variedbetween 18.8% and 33.5%. In accessions grown in ECRI proteincontentinseedsvariedbetween22.1%and33.5%.The research was funded by the European Union’s 7th FrameworkProgramme for research, technological development anddemonstrationundergrantagreementNo613781,EUROLEGUME

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P22–S4 GeneticDiversityofCroatianCommonBeanLandracesCarović-StankoK.1,2,LiberZ.2,3,VidakM.1,BarešićA.1,GrdišaM.1,2,RadosavljevićI.2,3,ŠatovićZ.1,21 - University of Zagreb, Faculty of Agriculture, Department of Seed Science andTechnology,Svetošimunskacesta25,HR-10000Zagreb,Croatia2-CentreofExcellenceforBiodiversityandMolecularPlantBreeding,Svetošimunska25,HR-10000 Zagreb, Croatia 3 - University of Zagreb, Faculty of Science, Department ofBotany,MarulićevTrg20/II,HR-10000Zagreb,Croatia*kcarovic@agr.hrInCroatiacommonbean isa traditionalgrain legume,grownbysmallfarmers in low input agriculture systems. The majority of theproduction is still based on local landraces adapted to the specificgrowing conditions and agro-environments and showing a greatmorphological diversity. Local landraces are in danger of geneticerosion caused by complex socio-economic changes in ruralcommunities. The low profitability of farms and their small size, theadvancedageof farmersandthereplacementof traditional landraceswithmodern bean cultivars and/or othermore profitable crops havebeenidentifiedasthemajorfactorsaffectinggeneticerosion.Three hundred accessions belonging to seven most widely usedmorphotypes (landraces)weregenotypedbymicrosatellitemarkers inorder to assess the genetic diversity and population structure ofCroatiancommonbeangermplasm.PCR-basedphaseolintypeanalysiswas used to trace the origin of accessions to Mesoamerican andAndean domestication centres. One-third of accessions showedphaseolin type I ("S"), predominant in germplasm of Mesoamericanoriginwhile the restof accessionsbelonged toAndeandomesticationcentre characterized by the phaseolin type II ("H" or "C"; 22%) or III("T"; 47%). A model-based structure analysis based on microsatellitemarkers revealed thepresenceof threeclusters, for themostpart, incongruencewiththeresultsofphaseolintypeanalysis.

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P23–S4 IdentificationofgenesinvolvedwithresistancetoFusariumoxysporuminchickpea(Cicerarietinum)ecotypesfromCentralItalyFierroM.1,DeCurtisF.1,RubioJ.2,MillánT.3,GilJ.3,LimaG.11-DipartimentoAgricoltura,AmbienteeAlimenti,UniversitàdegliStudidelMolise,ViaF.DeSanctis,86100Campobasso,Italy.2 - IFAPA Centro ""Alameda del Obispo"" Area de Mejora y Biotecnología, Avda.MenendezPidalApdo.3092,14080Córdoba,Spain3-DepartamentodeGenética,UniversidaddeCórdoba,CampusRabanalesEdificioC52aPlanta,14071Córdoba,Spain.Chickpea (Cicer arietinum L.) is a self-pollinating diploid cultivatedspecies genetically distinguished in two subpopulations: Desi andKabuli.Itisthethirdmostimportantgrainlegumeintheworldcovering11.5millionhaofcultivatedsurface. InItaly,theannualmeanyieldofchickpea is around 1.9t/ha-1 and the cultivated surface is actuallyincreasing.Thecrop ismainlycarriedoutbysmall farmerssothattheuseoflocalecotypesiswidespread.Duetodifferentselectivepressuresome of these ecotypes are often affected by serious pathogens asFusariumoxysporum f. sp. ciceris (Foc) thatoftenproduces significantreduction of yield. In this regard, the aim of this work was tocharacterize by pathogenicity tests in growth chamber and geneticmolecularanalysis theresistance toFocof18chickpeaecotypes fromcentralItaly.The pathogenicity tests evidenced that an ecotype (Longano) displaysfullresistancetoFoc,another(S.EliaaPianisi)showspartialresistanceandother16ecotypeswereallsusceptible.Geneticanalysisevidencedthe presence of a 6 ecotypes cluster, including the ecotype Longanoand distinguished fromothers ecotypes.Moreover, in some ecotypesnewallelesinthemicrosatellitesTA27andTA59wereidentified.Thesemicrosatellites are known to be associatedwith a gene involvedwithFocresistanceandlocatedonLG2ofchickpeageneticmap.

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P24–S4 Variabilityoffatcontentandfattyacidsprofilesinseedsofwhitelupin(LupinusalbusL.)collectionRybiński,W.1,Święcicki,W.1,Bocianowski,J.2,Barzyk,P.3,Börner,A.4,Starzycka,E.5,Starzycki,M.51-InstituteofPlantGenetics,PolishAcademyofSciences,Pozna_,Poland.2-UniversityofLifeSciences,Pozna_,Poland3-PlantBreeders/Wiatrowo,Poland.4-LeibnizInstituteofPlantGeneticsandCropPlantProduction,Gatersleben,Germany.5-InstituteofPlantBreedingandAcclimatization,DepartmentofOilPlants,Pozna,Poland.Thepaperpresentsanattempttoassessthevariabilityoffatcontentandfatty acids (FA) composition in seeds of white lupin (Lupinus albus L.)domesticcollection.The initialmaterialcomprisedaccessionsoriginatedfrom 30 countries. According to data given by accession donors thematerialwasdivided into fourgroupsoforigin:cultivars (CV), landraces(LR), wild relatives (CO) and cross derivates plus mutants (XD-M). Theaveragefatcontentforanalyzedaccessionsis9.81%.Fatcontentrangedfrom 6.9 % to 14.1%. In respect to unsaturated fatty acid (UFA),monounsaturatedoleicacidineachofestimatedgroupofaccessionswaspredominant andmost abundant (55.7%) in broad range of minimum-maximum values from 41.2% to 66.2%. The second examinedmonounsaturatedfattyacidwaserucicacid(1.74%)foundinseedsofallstudiedaccessions.ExceptionswerefourgenotypesinCOgroupdefinedsimilarly to rapeseeds as “zero erucic” forms. Among polyunsaturatedfattyacids(PUFA)dominatedlinolenicFA(ω–6)followedbylinolenicFA(ω–3).BothFAwereintherange13.7-33.2%and5.6-12.8%withmeanvaluesonthelevel19.6%and10.1%respectively.Asaconsequence,theexamined seeds showed a very favourable ω–3/ω–6 FA ratio (0.51),rangingfrom0.21to0.87,muchhigherthanthatofmostvegetableoils.Fat content was positively correlated with stearic and oleic fatty acidsandnegativelywithpalmitic,linolic,linolenicanderucicacid.

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P25–S4 Grainlegumeparticipatoryresearch:theAlvaiázeregrasspeaagronomictrialsexampleGonçalvesL.1,AlmeidaN.F.1,LourençoM.2,JuliãoN.3,VazPattoM.C.11 - InstitutodeTecnologiaQuímicaeBiológicaAntónioXavier,UniversidadeNovadeLisboa,Av.daRepública,2780-157Oeiras,Portugal.2-CâmaraMunicipaldeAlvaiázere,PraçadoMunicípio,3250-100Alvaiázere,Portugal.3-Simões&RamosLda,EstradaNacional110,3250-404RegodaMurta,Portugal.ThereisagreatpotentialtoreduceEuropeanexternaldependencyonplant proteins by the re-introduction of grass pea (Lathyrus sativus)into drought-prone areas. In Portugal, grass pea is part of thetraditional heritage of dryland communities and is helping torevitalizedryagriculturalregionssuchasAlvaiázere.Howeverduetoitsunderuse,hassufferedstronggeneticerosionandthereis lackofproductive,locallyadaptedvarieties.Under the scope of QUALATY project and in order to identifyinterestingsourcesof importantagronomic traits for this region,wegatheracollectionof150differentgrasspeaaccessionsfromalloverthe world (with 12 representatives of the national grass peatraditionalvarieties).Thiscollectionwastestedunderfieldconditionsusing a participatory α-latice design experiment involving farmers,researchersandthelocalgovernment.Accessionsarebeingevaluatedfor morphological and agronomic characters (passport data, plantdescriptors,agronomiccharacters,performanceandyield)andotherend-usersimportanttraits.Data will be submitted to a multivariate analysis, performing acomparativeassessmentoftheaccessionsbehaviorinthisregionandallow discriminate outstanding pre-breeding materials to beincorporated infuturegrasspeabreedingprograms.Theestablishedparticipatory research net will contribute to faster and wideradoptionoftheprojectresearchfindingsandelevatelocalknowledgetothestatusofscience.

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P27–S4 ProductionandphysicalcharacterizationofsixteencowpeaaccessionsFernandesH.A.1,AbadeF.2,CastroC.3,CarnideV.1,4,RibeiroC.J.31 - Centre for the Research and Technology of Agro-Environmental and BiologicalSciences(CITAB),UniversityofTrás-os-MontesandAltoDouro(UTAD),QuintadePrados,5001-801VilaReal,Portugal.hortenseaf@hotmail.com2-UniversityofTrás-os-MontesandAltoDouro(UTAD),QuintadePrados,5001-801VilaReal,Portugal.3 - University of Trás-os-Montes and Alto Douro (UTAD), Department of Agronomy,QuintadePrados,5001-801VilaReal,Portugal.cribeiro@utad.pt4 - University of Trás-os-Montes and Alto Douro (UTAD), Department of Genetics andBiotechnology,QuintadePrados,5001-801VilaReal,Portugal.Cowpea(VignaunguiculataL.)isanannualcropofwarmseasonmainlyproduced tropical and subtropical regions. However it is produced inMediterranean region, also. There are a lot of germplasm accessionsthatmustbeevaluatedbytheirproductivityandadaptationtospecificconditions. So, sixteen cowpea accessionswere sowed at Vila Real at2014seasontoquantifyproductionandphysicalattributeslikeweight,colorparametersandforcetocutthepodandtheseed.Thisworkwasperformedtoobtainpreliminaryresultsforpreparingthenextstepsforfurther selection and freezing objectives aiming the improvement oftheintakeofproteinfromvegetalorigininhumandiet.Themain results showed some differences among accessions in totalproduction and texture properties related to the cutting force,reinforcing the idea of different adaptation to the local conditions.Theseresultsrevealthepotentialuseofcowpeaasanalternativecropto other species and the need to study its behavior under freezingconditions.It was clear that some accessions presented highest amount ofproduction at the beginning of the harvest season while otherspresented a regular production along the season. There was enoughevidenceofalargeperiodoffloweringandaquitelongharvesttimeinthe accessions evaluated which is not favorable to a mechanicalharvest.

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P28–S4 Geneticdiversityofroot-nodulatingbacteriaisolatedfromGreekcowpeavarietiesFotiadisC.,NtatsiG.,SavvasD.,TampakakiA.DepartmentofCropSciences,AgriculturalUniversityofAthens,Athens,Greece.Cowpea (Vignaunguiculata) formsnitrogen-fixing rootnoduleswithslow-growing rhizobial species also named “cowpea miscellany”which generally belong to the Bradyrhizobium genus. Despite thatmuch research has been done on cowpea-nodulating bacteria invarious countries around the world, very limited information isavailableoncowpearhizobiainEuropeansoils.Giventheimportanceof cowpea in sustainable agriculture and the lack of data on theirrhizobia in Europe, we performed for first time an analysis on thephylogeny and genetic diversity of indigenous cowpea-nodulatingrhizobia in Greece. The diversity and phylogeny of isolated strainswereinvestigatedbyERIC-PCRfingerprintingandmultilocussequenceanalyses (MLSA) of eight housekeeping genes. Phylogeny ofnodulation (nodC)andnitrogen-fixation (nifH)genesof rhizobiawasalsostudied.Thesensitivityofthestrainstodifferentantibiotics,theirtolerance to different levels of salinity, pH, temperature and theirability to nodulate soybean and common bean plants were alsoassessed. Based on ERIC-PCR fingerprinting andMLSA analyses, theisolated strains were grouped into four well-supported clustersbelonging to the branch of the generaBradyrhizobium, Ensifer, andRhizobium. Finally, our results indicated that some of the strainsstudied might represent novel species, indicating that an as yetunknowndiversityofrhizobiamayexistinGreeksoils.

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P29–S4 MolecularcharacterizationoftheU.S.PhaseolusacutifoliusA.GraycollectionusingAmplifiedFragmentLengthPolymorphism(AFLP)andTargetedRegionAmplificationPolymorphism(TRAP)markersKishaT.1,BhardwajH.L.21-USDA-ARS2-VirginiaStateUniversityTepary bean (Phaseolus acutifolius A. Gray) is a short life-cycle annualdesert legume indigenous to northwestern Mexico and southwesternUSAandisconsidereddroughtandheattolerant.TheWesternRegionalPlant Introduction Station currentlymaintains 211 accessions of teparybean. Molecular genetic relationships among 195 accessions of thePhaseolus acutifolius A. Gray collection were assessed using TargetedRegionAmplifiedPolymorphic(TRAP)markersdesignedfromsequencesof genes associated with heat and drought tolerance and randomAmplifiedFragmentLengthPolymorphic (AFLP)markers.AlthoughTRAPmarkerstargetspecificgenes,theyhavebeenshowntoproducerandomgeneticdifferencesamongaccessions.Indeed,thecorrelationamongthematrices produced by eachmarkerwas R = 0.81. Genetic relationshipswere compared to drought stress using measurements of variablefluorescence to maximum fluorescence (Fv/Fm). Cluster analysis usingNTSys-pc and STRUCTURE of the combined marker set found 3 majorgroups and 7 sub-groups, but there was no association of droughttolerance with any group. Although the tepary bean has been used ininterspecific crosseswith commonbean for heat tolerance and diseaseresistance, accessions have also been identified with prolific seedproduction,whichcouldalsoincreaseyieldincommonbean.

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P30–S4 StructureofgeneticdiversityinaworldcollectionofwhitelupinlandracesNazzicariN.,AnnicchiaricoP.,FerrariB.Council forAgricultural Research andEconomics (CREA), ResearchCentre for FodderCropsandDairyProductions,29vialePiacenza,26900Lodi,Italy.White lupinwasdomesticated in theAegean islands, fromwhere itspread across southern Europe, North and West Africa, and NearEast. Itsgeneticresourcesare limitedtotheprimarygenepool.Therescue of white lupin as a major grain legume, justified by itsoutstanding protein content, is hindered by limited breeding workthat suffers, inter alia, from lack of information on the structure ofcrop genetic diversity. The aim of this study was investigating thestructureoflandracegeneticdiversityacrossdiversitylayersrelativeto variation among: (i) seven major cropping regions (Madeira-Canaries,Portugal,Spain,Maghreb,Egypt,EastAfrica,NearEast),(ii)landraces within cropping region (8-10 sampled landraces perregion), and (iii) genotypeswithin landrace (3-4 sampled genotypesper landrace). Overall, 243 genotypes were characterized by agenotyping-by-sequencingapproachthatprovided2178polymorphicSNP markers per genotype (for a 10% genotype missing datathreshold).Ananalysisofmolecularvarianceindicatedthefollowingrankingofvariancecomponents:genotypevariationwithin landrace> landracevariationwithin region>variationbetween regions. Thisresult, not expected for this predominantly self-pollinated crop,highlightstheimportanceofexploitingalsowithin-landracediversity.Landrace multi-dimensional scaling ordination displayed mainly alatitudinal gradient, along with outstanding variation within NearEast.

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P31–S4ConservationandregenerationoftheannualpasturelegumesandLupinusspp.SpanishactivecollectionsMaya-BlancoV.,Gragera-FacundoJ.,Lozano-RuizM.J.,González-LópezF.CICYTEX,JuntadeExtremadura,SpainIn the germplasm bank of the Instituto de Investigaciones AgrariasFincaLaOrden-Valdesequera(CICYTEX)therearetwoofficialnationalactiveseedcollections: theannualpasture legumesand theLupinusspp.collections,including6290and1950accessions,respectively.Basic issues of this germplasmbank are preservation,multiplicationanddocumentationoftheiraccessions.Aboutpreservation, theconditionof thestoredsampleseedsof theaccessions are being revised every year changing hermetic seals orregeneratingsilicagelifnecessary.Furthermore, the multiplication of the accessions that are lowgermination capacity or their stored samples have less than 4000seeds (2000 for Lupinus spp.) or are not duplicated in the basecollection (CRF) or are new accessions are going on. Along thesemultiplications, the new accessions are characterized and basicallyevaluated.For documentation, the germplasmdatabases are beingmaintainedandupdated.

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P32–S4 AssessmentofgeneticdiversityofsoybeanaccessionsusingSSRmarkersPericV.1,NikolicA.1,ZoricM.2,MikicA.2,DrinicS.M.11-MaizeResearchInstitute“ZemunPolje”,Belgrade,Serbia2-InstituteofFieldandVegetableCrops,NoviSad,SerbiaSoybean is a plant species characterized by an extremely narrowgeneticbase,asaconsequenceofself-pollination,alongprocessofdomestication and artificial selection. A key phase inmaintainingdiversity and successful utilization in breeding is the genetic andphenotypic characterization of accessions available in collections.Soybean collection in Maize Research Institute "Zemun Polje"maintainsmore than500accessions fromdifferent regionsof theworld.Theaimofthisstudywastoinvestigatethelevelofgeneticvariation in collection, through the sample of 90 soybeangenotypesoriginatedfrom15countries,classifiedin5geographicalgroups (DOM-Serbia, EUR-European, USA-North American, CAN-Canadian, EXO-China and Japan). Twenty SSR primer pairs wereselectedforthemolecularanalysis.Toassessthegeneticrelationsamongaccessions,clusteranalysisemployingUPGMAmethodwasperformed.Distributionofgeneticvariationbetweenandwithin5groupswascalculatedbyAMOVA(analysisofmolecularvariance).MolecularanalysisrevealedamoderatepolymorphismofSSRloci.Groupingpatternshowedahigh levelofagreementwithpedigreedata, and, to a certain extent, with geographical origin ofgenotypes. AMOVA for five geographical groups of genotypesshowed that low, but highly significant portion of variation hasbeen attributed to differences between groups. Canadiangenotypes were significantly differentiated from all observedgeographicalgroups.Nosignificantgeneticdifferentiationbetweendomestic and USA group of genotypes was observed, as well asbetweenDOMandEURgroupofgenotypes.Acknowledgement: The project TR-31068 of the Ministry ofEducation,ScienceandTechnologicalDevelopmentoftheRepublicofSerbia

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P33–S4 Temporalandregionaldevelopmentoflentilpopulationsbynaturalselectionon-farmRulandM.,BeckerH.C.,HorneburgB.Georg-August-UniversitätGöttingenOn-farm management of plant genetic resources allows forevolutionary adaptation to site-specific conditions by naturalselection. However, knowledge about the development of geneticdiversity by on-farm management at multiple sites is still scarce.Lentil isanautogamousspecieswithanoutcrossingrateof0tofivepercent.Inalong-termexperiment,threeoldlentilcultivarswereexposedtoten generations of natural selection at three farms under rainfedconditions.Twooftheselectionsiteswereonpoorormarginalsoils.Populationsingeneration0,5,and10weretestedinfieldplotsatallthreeselectionsites.Resultsindicatedi)highermeanyieldforthe10thgeneration,ii) no significant site-specific adaptive effect (“home fieldadvantage”)onyield,butiii) an overall higher yield for seed and straw of populationsdevelopedatthemoststressproneselectionsite;iv)significantsite-specificchangesinseedweightforbothlargerandsmallerseedswereobservedforonecultivar.Seedweight,leafsize,andfloweringtimeweretraitscontrastingthepopulations of different generations and provenances within thecultivars.GenotypingwasdonewithSNPmarkers(KASPar)forallpopulationsbyusingoneseedeachof100singleplantprogeniesperpopulation.Calculationsforgeneticdiversityparametersareinprogress.

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P34–S4 PerspectivesofpulsesgeneticresourcescollectioninPortugalBarataA.M.1,ReisA.1,GasparC.1,RochaF.1,LopesV.1,PereiraG.2,DuarteI.21-BancoPortuguêsdeGermoplasmaVegetal/INIAV,I.P.,QuintadeSãoJosé,S.PedrodeMerelim,4700-859Braga,Portugal2-InstitutoNacionaldeInvestigaçãoAgráriaeVeterinária,I.P.(INIAV).Apartado6,7350-951Elvas,PortugalPulsesaremultifunctionalcropswith large importance foragriculture,environment and culture in the Mediterranean countries. Today, inPortugaltheaverageconsumptionofpulses/percapitais4Kg.Pulses genetic resources collection in Portugal, contains 6,359accessions from nine species included in the Annex 1 of theInternational Treaty on Plant Genetic Resources for Food andAgriculture.Pulsesgeneticresourcescollection,comprisesgeneticmaterial,farmerknowledgeandheritageprofile.Thecollectionbegan4decadesago,asaresultof126collectingmissionsandassessmentoflocalpracticesanduses.Thepulsecollectionwasevaluated formorphological,molecularandbiochemicalmarkers.Breedingprogramshavebeen implementedand led to the registration of new varieties In the National VarietiesCatalogue.All the results are documented and the passport data is in the publicdomainthroughtheGRIN-GLOBALplatform.Thepulsesnationalcollectionhasavaluablerole increatingadiverseeconomicallyviableagro-foodvaluechain:Organicfarming;SustainableIntensiveagricultureprograms,helpingtoaddresschallengesposedbyclimatechanges;DevelopmentofnewproductsassociatedtonewusesintheMediterraneandiet.The genetic basis of pulses in our agricultural landscapes can beenlarged by promoting genetic materials more resilient andreintroducing traditional varieties in the value chain, making themcommerciallyviable.

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P35–S4GeneticdiversityoftheguatemalanclimbingbeancollectionTobar-PinonM.G.1,2,McCleanP.E.1,MoghaddamS.M.1,LeeR.1,OsornoJ.M.1,Villatoro-MeridaJ.C.21-DepartmentofPlantSciences,NorthDakotaStateUniversity,Fargo,USA2-InstitutodeCienciayTecnologíaAgropecuaria(ICTA),GuatemalaCommon bean (Phaseolus vulgaris L.) is the most important legumecropforhumanconsumptionintheworld,andistheprimarysourceofprotein in thedailydiet inCentralAmerica. InGuatemala, itoccupies184,000hectaresor17.8%ofavailableproductionacreagewhere8of10nativechildrensufferfromchronicmalnutrition,thehighestlevelinLatin America. For this reason, food security plays a key role in thedevelopment of a healthy country. At this point, bean breeders arechallenged to increase seed yield and maintain seed quality whilebreeding for resistance to several diseases whose incidence hasincreased due to climate change. Race Guatemala of common beanincludes climbing beans from the highlands of Guatemala wherepoverty is the highest, and it is a resource of new alleles for beanimprovement. The objectives of this research are to evaluate anddescribe the population structure, genetic diversity, and geneticdifferentiationofaGuatemalanclimbingbeancollectionof376inbredlines using SNPmarkers, and to perform a genome-wide associationstudytomapimportantagronomictraits.Thepopulationstructurewillalso be characterized by neighbor-joining and principal componentsanalyses, and differentiation will be estimated by FST, and expectedheterozygosity(He).

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P36–S4CharacterizationofgeneticdiversityofaEuropeancollectionofPea(PisumsativumL.)BarcelosC.,PereiraG.,MenesesM.,DuarteI.,InstitutoNacionaldeInvestigaçãoAgráriaeVeterinária,I.P.(INIAV)Apartado6,7350-951Elvas,PortugalIn order to integrate the germplasm collections in the plant breedingprograms, the characterization and evaluation of genetic diversity isessential. INIAV participates in the FP7 project EUROLEGUME, whereone of the main objectives is evaluate the performance of the localgenetic resources of pea accessions. Seventy-six pea accessionsprovidedby thepartners INIAV (Portugal),AREI (Latvia), ECRI (Estonia)and one reference variety from INRA-France were evaluated in theexperimentalfieldofINIAV(38°53’N,7°09’W).Thecharacterizationwasperformedusingthreequalitativetraits(plantpigmentation,flowerandseed colour) and six quantitative traits (days to flowering and tomaturity,durationof flowering,plantyield,100seedweightandplantheight). Datawere analysed by numerical taxonomy techniques, usingNTSYS-pc package, version 2.01.The results showed that there isconsiderablemorphologicalvariationamongthegenotypes.Concerningthequantitativetraits,themostvariablecharacterwas100seedweight.The number of days to start flowering is negatively correlated withdurationoffloweringperiodandplantyield.Theaccessionsthatneededmore days to start the flowering stage are from Latvia. In Portugalconditions, themostpromisingpea accessions that canbe included inbreedingprogrammeare thePortuguese varietyGrisel, the accessionsErme,Mehis,Seko,Tasuja,Herko,LoomingfromEstoniaandk-6972,k-4822,k-6973fromLatvia.

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P37–S4 MorphologicalcharacterizationofaEuropeancollectionoffababean(ViciafabaL.)PereiraG.,BarcelosC.,MenesesM.,DuarteI.InstitutoNacionaldeInvestigaçãoAgráriaeVeterinária,I.P.(INIAV)Apartado6,7350-951Elvas,PortugalFaba bean is an important legume cropwell adapted tomost climaticareas of Europe and widely used for feed and food. In Portugal, themajor objectives of faba bean breeding programme are to developvarieties with high yield potential, early maturity and tolerant to themajordiseases(BotrytisfabaeandUromycesfabae). INIAVparticipatesin the FP7project EUROLEGUME,whereoneof themain objectives isevaluate the performance of the local genetic resources of faba beanaccessions. 16 faba bean accessions provided by the partners INIAV(Portugal), AREI (Latvia), ECRI (Estonia) and PHRC (Latvia) wereevaluated in order to identify accessionswith particular behavior thatcould be exploited by plant breeders. The faba bean experiment wasconducted in the experimental field of INIAV-Portugal (38°53’N,7°09’W). Characterization was performed using six quantitave traits(daysto floweringandtomaturity,durationof flowering,plantheight,plantyield,100seedsweight)andthreequalitytraits(flowerandseedcolour, growth habitat). Data were analysed by numerical taxonomytechniques,usingNTSYS-pcpackage,version2.01.Inthiscollection,themajor accessions were more productive than minor accessions.Portuguese accessions originated shortest plants than the accessionsfrom Latvia and Estonia. Themost promising faba bean accessions forfurtherevaluationarePortugueseaccessionsFb3268,Fb395,Favel,Fb2939andtheaccessionsValmiera,ToleafromLatvia."

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P38–S4 Anovelsourceofgeneticdiversityincultivatedchickpeaasrevealedbygenome-widemarkerdiscoveryandgenotypingLottiC.1PavanS.2,MontemurroC.2,DeGiovanniC.2,RicciardiF.1,BardaroN.2,MazzeoR.2,SonnanteG.3,MarcotrigianoA.R.2,RicciardiL.21 -Dept.of theSciencesofAgriculture, FoodandEnvironmentUniversityof Foggia,ViaNapoli25,I-71100Foggia,Italy2–Dept.ofSoil,PantandFoodSciences,SectionofGeneticsandPlantBreeding.FacultyofAgriculture,UniversityofBari.ViaAmendola165/A,70126Bari,Italy;3-InstituteofBioscienceandBioresources-RNC,ViaCelsoUlpiani,70125-BariTheaccuratedescriptionofplantbiodiversityisofutmostimportancetoefficiently address efforts in conservation genetics and breeding. Herewe report the successful application of a genotyping-by-sequencing(GBS) approach in chickpea, resulting in the characterization of acultivatedgermplasmcollectionof82accessionswith3187high-qualitysingle nucleotide polymorphism (SNP) markers. A body of evidence,based on genetic structure, principal component, and hierarchicalclusteringanalyses,indicatetheidentificationofapreviouslyunnoticedgene pool, corresponding to black-seeded genotypes traditionallycultivatedintheSouthEastofItaly.Thispooladdsuptotheothertwocharacterized by previous literature and corresponding to thecommercial seed types desi and kabuli. Fixation index estimates forindividualpolymorphismshighlightlociandgenomicregionsthatmightbe of significance for the diversification of agronomic and commercialtraits. Overall, our findings provide information on the geneticrelationships in cultivated chickpea and identify germplasm of greatinterest for the scientific community and chickpea breeding, which islimitedtoagreatextentbynarrowgeneticdiversity.

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P39–S4 MedicagotruncatulaexhibitsanoveltypeofquantitativediseaseresistancetoVerticilliumwiltinvolvinganetworkofco-regulatedgenesrelatedtoPAMP-triggeredimmunityBenC.1,ToueniM.1,LeRuA.2,GentzbittelL.1andRickauerM.11-EcoLab,UniversitédeToulouse,CNRS-INP-UPS,Toulouse,France2 - Research federation « Agrobiosciences, Interactions et Biodiversité », 24 Chemin deBordeRouge,BP42617,31326Castanet-Tolosan,FranceMolecular and cellular responses to Verticillium alfalfae wereinvestigated inthemodel legumeMedicagotruncatula.Whereas initialrootcolonizationwassimilarinresistantandsusceptiblelines,resistantline A17 eliminated the fungus before 7 days post-inoculation, incontrast to susceptible F83005.5 that became heavily colonized. Atranscriptomic study of root response during early stages of theinteractionshowedthatlineA17hadhigherbasalexpressionofdefenserelatedgenes,andrespondedtoinoculationwithup-regulationofonlyasmall number of genes. The susceptible line in contrast exhibited adisorganized response involving a large number of up- and down-regulatedgenes fromdifferent functional classes.Hormonal control ofresistance, as hypothesized from gene expression patterns, wasexperimentallyassessed.Aco-expressionnetworkanalysishighlighted5modulesofco-expressedgenesintheresistantline,whereasnosuchstructuredgeneexpressionwas found in the susceptible line. One module was particularlyassociatedtotheinoculationresponseinA17,containingthemajorityofthe genes induced in A17 and notably genes associated to PAMP-triggeredimmunity.Eleventranscriptionfactorsfromthismoduleweredescribed to respond toother root pathogens inM. truncatula. Takentogether, the results suggest that resistance inM. truncatula line A17mightbedue toPAMP-triggereddefensemechanisms,whichadd to apre-existinghighbasaldefenselevel.

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P40–S4 AutoecologyoflegumespeciesfromfivegenerainAlgeriaandpossibilitiesofrecoverytoimprovethepastoralproductionAbdelguerfiA.1,LaouarM2.1 -E.N.S.ALaboratoired’Amélioration IntégrativedesProductionsVégétales,El-Harrach,Alger,Algérie2 - E.N.S.A LaboratoiredesRessourcesGénétiquesetBiotechnologies, El-Harrach,Alger,AlgérieAlgeriaischaracterizedbygreatdiversityofenvironments.Variationsintemperature, altitude, rainfall (amount and distribution), soil type(texture, salinity), fashioned plants with very specific adaptations.Knowledgeoftheseadaptationswouldbeamajorasset inthecontextof the valorization of these genetic resources. The autecology anddistributionofspeciesfromfivegenera,Medicago,Trifolium,Scorpiurus,Hedysarum and Onobrychis, were studied. Several bio-prospectionswereconductedinAlgeriaandon749sitesautecologywascarriedout.According to the genus, the main effect ecological factor of thepresence-absenceofspecieschange.Theecologicalneedsandlimitsofspecies could be applied for the development and/or improvement ofpasturesandfallow.Additionaltotheecologicalaspects,theadaptationandstabilityproductionofspeciesweretakesintoaccount.

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P41–S4 PhenotypicVariabilityforAgromorphologicalTraitsinaLentilRecombinantInbredLinePopulationTokluF.,ÖzkanH.,KaraköyT.,HatipoğluR.CukurovaUniversityAgriculturalFaculty,FieldCropsDepartment,01330,Adana,Turkey.fapet@cu.edu.trLentilisoneoftheimportantmembersoffoodlegumes,hassignificantrole in human nutrition and animal feed in the world. Turkey is theprimary gene center of lentil, contrary to this there is very limitedresearch done for development of cultivar with high efficiency, highquality,andsuitabilityformechanizedharvesting.Due to above mentioned reasons, the objective of this work are todevelop linkage map saturated with genomic SSR markers and toidentify the QTLs for agromorphological and quality traits. In thismanuscrip, only agromorphological traits evaluated because of thegenomic studies are going on. One hundred fifty five recombinantinbred lines (RILs) at the F8 stage developed through single seeddescentmethod from cross between two Turkish red lentil landracesKaracadağ x Silvan were used as plant material. 155 recombinantinbred lines were grown at two locations (Adana/Kozan, Sivas)accordingtoAugmentedblockdesigninthefieldexperiment.In conclusion, considerable diversity was observed for the days toflowering,floweringduration,daystomaturity,plantheight,biologicalyield, number of the pods per plant, grain yield per plant, seeddiameterand1000grainweightatbothlocations.

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P42–S4 PhenotypicvariabilityforagromorphologicaltraitsinalentilrecombinantinbredlinepopulationÖzkanHÇukurovaUniversity,TurkeyLentilisoneoftheimportantmembersoffoodlegumes,hassignificantrole in human nutrition and animal feed in the world. Turkey is theprimary gene center of lentil, contrary to this there is very limitedresearch done for development of cultivar with high efficiency, highquality,andsuitabilityformechanizedharvesting.Due to above mentioned reasons, the objective of this work are todevelop linkage map saturated with genomic SSR markers and toidentify the QTLs for agromorphological and quality traits. In thismanuscrip, only agromorphological traits evaluated because of thegenomic studies are going on. One hundred fifty-five recombinantinbredlines(RILs)attheF8stagedevelopedthroughsingleseeddescentmethodfromcrossbetweentwoTurkishredlentillandracesKaracada_xSilvanwereusedasplantmaterial.155recombinantinbredlinesweregrown at two locations (Adana/Kozan, Sivas) according to Augmentedblockdesigninthefieldexperiment.In conclusion, considerable diversity was observed for the days toflowering, floweringduration,days tomaturity,plantheight,biologicalyield,numberofthepodsperplant,grainyieldperplant,seeddiameterand1000grainweightatbothlocations.

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Session 5, parallel: Legumes value chain: marketrequirements and economic impact (cont.) - RoomArrábidaIIIChaired by FrédéricMuel (Terres Inovia, France) and PeteIannetta(JHI,UL)

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OralCommunications16:30-16:40 Oral–S5Evaluatingcereal-legumeintercropstowardssolecropsbycombiningargumentationandsimulationThomopoulosR.1,BedoussacL.2,MoulinB.31-INRA,IATEJointResearchUnit/INRIAGraphIK,Montpellier,France2-AGIR,UniversitédeToulouse,INPT,INP-PURPAN,INRA,ENFA,31320,Auzeville,France3-LavalUniversity,Quebec,CanadaIntercropping(IC)cerealandlegumeisapracticeparticularlysuited inlownitrogen input systemswhere itoptimizes theuseofN resourcesleading to improvedandstabilizedyieldsand increasedcerealproteincontent[1].Nevertheless,ICisonlyslightlyadoptedbyfarmers.Indeed,their potential economic advantage remains questionable because itdepends onmany factors (crop prices, cost to separate the grains orinputpricesandsubsidies).In the context of decision support [2], our work aims at proposing asystematic approach to assess various options available to farmerscombining: i) a qualitative model based on arguments expressed byactorsandii)aquantitativesimulationtechniquebasedonsystemstocomparedifferentscenarios.Simulationswereperformed toassessandcompare thedirectmarginexpectedusingactualobservations and then3 independent scenariosunder the following “what if” hypotheses: i) same subsidies, ii)increasedcostofinputsandiii)decreasedcostofsorting.Thisstudyillustratestheinterestoftheproposedapproachandopensnew perspectives, such as including information about product price,fertilizerpriceandLifeCycleAssessmentdata inorder tohelp for thesupport of these alternatives in a context of price volatility,environmentalconcernsandclimatechange.[1] Bedoussac et al., 2015. Agronomy for Sustainable Development35(3):911-935[2] Thomopoulos R., Croitoru M., Tamani N., 2015. EcologicalInformatics26(2):182

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16:40-16:50 Oral–S5Escapingfromgrain-legumesocio-technicalsystemlock-in:someevidencefromsocio-economicinnovationtrendsinFrance.MagriniM.INRA,24ChemindeBordeRouge-CS52627,CastanetTolosan,31326,FranceGrain-legumescanpotentiallydecreaseglobalwarming,astheydonotneednitrogenfertilizersthatareresponsibleforhalfofallagriculturalgreenhousegasemissions.Asdiversifyingcropsinthesimplifiedarablesystems in France, they also allow to decrease numerousenvironmental impacts of these systems. Moreover, grain-legumeshavemanyfunctionalandnutritionalpropertiesbothasfeedandfood.But despite those benefits, grain-legumes prouction continues to fall.Someauthorsexplain this situationasa technological lock-in thathasresulted from the co-evolution of cropping systems towardhomogenization and intensive use of agrochemical inputs, publicpolicies,andmarketdynamicsthatpromotedcerealsduringthehalfofthe 20th century. Mobilizing theories on transitions from economicsand strategicmanagement, the objective of this communication is toreview the current main drivers that could allow escaping from thislock-in, and to analyze several socio-technical niche innovations, bothat theupstreamandthedownstreamof thesupplychains, thatcouldfostergrain-legumesproduction.Inparticularattentionwillbegiventothewaythoseinnovationscouldcreatemorevalueforthesecropsandso, to develop higher incentives for farmers to cultivate them. Thoseinnovations case studies are focus on France but the insights of thisanalysiscouldbeconsideredmorelargelyattheEuropeanscale.

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16:50-17:00 Oral–S5AnalysisofsocialandorganizationalaspectsoffoodlegumeschainBentaibiA.InstitutNationaldelaRechercheAgronomique,MorrocoThespecificobjectivesofthisworkaresummarizedin:i)analysisoftheroles and tasks according to gender in the production system; ii)identification of forms of collective and individual organization tomanageproductionsystems;iii) identificationoftheOPAabletodrivetheactionsoftheinitiativeandcontributetotherehabilitationoffoodlegumes.Theapproachisbasedonamethodologybasedonthecombinationofan analysis of the existing documentation and participatory researchthrough workshops and discussions with farmers and agriculturalorganizations present in the study area represented by five sitesdrivers.Despite their importance, theauthoritieshavenotpaidmuch interestinthedevelopmentofthesecrops.Theywereconsideredsecond-ratecultures and their interest is limited to their role as preceding crop.While legumes are cash crops that generate substantial benefits toruralhouseholds.Throughtheirrehabilitationispossibletorehabilitateforms of social organization that can play a crucial role in theirdevelopment.Small producers account for a majority of farmers and contributestrategically to foodsecurity,but theirdevelopment is still limitedby:(i) lack of access to capital for investment, (ii) the uncertainty of themarkets in which they changing and (iii) the lack of institutions andorganizations,includingcollectiveactionsforreturnoninvestment.

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17:00-17:10 Oral–S5There-innovationofMixedCropping–whocares?–TrialWillingnessamongGermanfarmersLemkenD.UniversityGöttingenMixed cropping (MC), thegrowingof twoormore coexisting crops inone field, specifically the mix of cereal and grain legumes, cancontributetoamoresustainableagriculturallanduse.Despiteavarietyofecologicalbenefitsandpromisinggrainproductivity,applicationsarescarce among German farmers (ca. 0.007 % of land use). R&D andagriculture machineries evolve around monocultures. Manystakeholders believe substantial technical barriers to hinder theindustrializationofMC. In considerationofMC’spotentialweanalyzefarmer’strialwillingnesstoidentifyaprofileof“earlyadopters”withinthefarmingsector.Thesubsequenttelephoneinterviewswerequotedin respect to a representative geographical distribution of farmerswithin Germany. A proportional odds model regressed thehypothesized drivers upon the gradual change to carry outMC-trials.Preliminary results point to a significant role of land ownership andprior adoption of legumes. The latter implies a strong dependenceofMC’s implementation on legume adoption pattern. The perception oftechnicalbarriersandtheperceptionofMC’susefulnessarealsomajordrivers.Researchandvisionaryagribusinesseswillneedtoidentifytheimplementation costsof the technical barriers andquantifymonetaryadvantagesoftheMC-approachbeforeacommunicationalstrategyforMC can and should be developed, otherwise diffusion will remainmarginal.

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Session6,parallel:Rootdiseases-RoomArrábidaIVChaired by Julie Pasche (North Dakota St. Univ., USA) andNicolasRispail(CSIC,Spain)

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OralCommunications16:30-16:40 Oral–S6Insightsintotherelativecontributionofmicro-evolutionandphenotypicplasticityinthequantitativeresponseofthemodellegumeM.truncatulatoverticilliumwilt.Gentzbittel,L.1,BenC.,1SbeitiA.1,MazurierM.1,ToueniM.,NegahiA.,SarrafiA.,BenameurS.,TardinM.-C.2,GrasM.-C.2,RickauerM.11-EcoLab,UniversitédeToulouse,CNRS,INPT,UPS,Toulouse,France2-R2n–CentredeRechercheLeBourg,12510Druelle,FranceThemodel legumeMedicago truncatula was used as a host to studyresistancetoVerticillium,asoil-bornepathogenthatcausessubstantialyield losses in many crops including alfalfa (Medicago sativa).Verticillium wilt response in M. truncatula is a quantitative diseaseresistance, regulated by QTL differing across resistant accessions andvaryaccordingtothefungalstrains,asrevealedbyageneticanalysisinamulti-cross,multi-strain,multi-sitedesign.ThissuggeststhatdistinctgeneticmechanismscontrolVerticilliumwiltresistance.To deepen the analysis, we assessed the genetic variability ofsusceptibility to Verticillium within the M. truncatula species, in acollectionof 237 lines fromaround theMediterraneanBasin.Diseasefunctional parameters and plant colonization by the fungus allowedidentifying the genomic localization of the most frequent resistancealleleswithinthespecies.The capacity of pathogens to adapt to new environments is a well-knownthreattodurabilityofcropresistance.Usingacommon-gardenexperimentingreenhouse,weshowedthatmoderateincreases(+3°C)ordecreases(-5°C)oftemperatureduringinfectionsignificantlyimpactearlyresponse,butnotfinalfitness,suggestingplasticityinresponsetothepathogen.We also experimentally adapted a V. alfalfae strain to highertemperature and show that increase in aggressiveness is rapid andefficient, confirming that climate change may quickly affect plant-microbeinteractions.

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16:40-16:50 Oral–S6GeneticsofpearesistancetoAphanomyceseuteichesinthegenomicsEraPilet-NayelM-L.1,DesgrouxA.1,LavaudC.1,LesnéA.1,BoutetG.1,AubertG.2,McGeeR.J.3,CoyneC.J.4,BourionV.2,BurstinJ.2,BarangerA.11-INRA-UMR1349IGEPP,PISOM-UMTINRA/TerresInovia,France2-INRA,UMR1347Agroécologie,France3-USDA-ARS,GrainLegumeGeneticsPhysiology,USA4-USDA-ARS,PlantGermplasmIntroduction,USACommonrootrot,causedbytheoomyceteAphanomyceseuteiches, isa major soil borne disease of pea in many countries. The large peagenomic and plant resources recently developed have deepened theknowledgeofgeneticsofpartialresistancetoA.euteiches.Adedicatedpanel of Aphanomyces genetic resources genotyped with a 13K-SNParray(Tayehetal,2015)wasusedinagenome-wideassociationstudy(GWAS)ofpartialresistance.Resultsidentifiedatotalof52smallsizedLD-basedconfidenceintervalsassociatedwiththeresistance,including14 consistent QTL regions at which marker haplotypes were definedfrom the panel (Desgroux et al, 2016). This study validated six of theseven main resistance QTL previously identified, for which Near-Isogenic-Lines (NILs) were created by back-cross assisted selection(Lavaudetal,2015).ThefingerprintoftheNILsusingthe13K-SNParrayshowed that QTL introgressions could cover several smaller GWASintervals, suggesting the presence of several linked genes or a singleimpreciselylocatedlocusunderlyingaQTL.Thegenomiclocationofthemajor QTL Ae-Ps7.6, which effect was confirmed on slowing downsymptomappearanceandrootcolonizationbythepathogen(Lavaudetal,2016),wasrefinedbyGWASandfinemapping.Theunprecedentedgenomic resources recently developed in pea provide new markersclosely-linkedtoAphanomycesresistanceloci,foracceleratingprogressinbreedingfuturepearesistantvarieties.

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16:50-17:00Oral–S6Progressonunderstandinggenetic resistance toFusariumrootrotinpeaPorterL.1,BoutetG.2,Pilet-NayelM.-L.2,BarangerA.2,McGeeR.1,MaY.3,CoyneC.41USDAARSGrainLegumeGenetics,Pullman,WAUSA;2INRA;3WashingtonStateUniversity;4USDAARSWesternRegionalPlantIntroductionStation.SeveralspeciesofFusariumcauserootrotofpea,amajorconstraintforpea production. One species, Fusarium solani f.sp. pisi has been animportant pathogen in the U.S., Canada and New Zealand productionregions. Infested fields result in stunting, yellowing and necrosis ofsusceptibleplants.HighlevelsofusefulpartialresistanceisavailableinpeagermplasmforthisFusariumrootrot,butthemultigenicresistancepresents a challenge for population improvement in pea breedingprograms. Two recombinant inbred line populations, segregating forFusarium root rot resistance, were phenotyped. One was screenedunder field conditions and the other in controlled conditions. Usingcomposite interval mapping, six QTL were identified controlling asignificantproportionofthevarianceandtwoQTLwereidentifiedunderboth conditions. Use of the discovered co-dominant flankingmarkersforimprovingFusariumrootrotresistancewillbediscussed.

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17:00-17:10 Oral–S6MolecularquantificationofpathogenicFusariumspp.insoiltopredictpearootrotriskinthefieldChattertonS.1,HeynenM.1,SafarieskandariS.2,Zitnick-AndersonK.3,PascheJ.S.3.1 - Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre,Lethbridge,ABT1J4B1,Canada.2 - Department of Agricultural, Food and Nutritional Science, University of Alberta,Edmonton,ABT6G2P5,Canada.3 - Department of Plant Pathology, North Dakota State University, Fargo, ND 58108,U.S.A.Root rotof fieldpeacauses severeyield loss in theCanadianprairies,where1.5millionhectaresofpeasaregrownannually.Arecentsurveyrevealedthat40%of fieldswerepositiveforAphanomyceseuteiches,while80-90%werealsoinfestedwithFusariumspp.PathogenicitytestsindicatedthatwhileA.euteicheswasthemostdamaging,F.avenaceumandF.solaniwerealsohighlyvirulent.Toassessriskofrootrotpriortoplanting peas, the relationship of DNA quantity of Fusarium spp. toinoculum potential and disease severity was evaluated. Soil wasinoculatedwithincreasingconcentrationsofFusariuminoculum,andanaliquotwasremovedforDNAextractionspriortoplantingpeasforrootrot assessments. DNA quantity in soils wasmeasured using real-timePCR assay, and F. avenaceum and F. solani DNA concentration wascorrelated to disease severity. This assay was then used to quantifyDNA in soil and stubble collected from commercial field samples andrelated to root rot severity recorded during the growing season. F.avenaceumwas detected in soil at concentrations that donot inducedisease. However, concentrations in stubble from previously growncropswereatdisease-causinglevels.SimilaranalysisisunderwayforF.solani.Resultsarebeingvalidatedinpeafieldsduringthe2016growingseason, and will be used to develop a soil test to predict risk ofdeveloping Fusarium root rot, in conjunction with A. euteichesquantification.

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Session 7, plenary: Legumes in food and feed andotheralternativeuses-RoomArrábidaI&IIChairedbyMaria CarlotaVaz Patto (ITQBNOVA, Portugal)andAmbujB.Jha(Univ.Saskatchewan,Canada)08:30-09:00Key lecture: -FrédéricMarsolais:Usingbeanswith novel protein compositions for nutritionalimprovement

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OralCommunications09:00-09:15 Oral–S7ThehiddenphenoliccontentoffababeansBentodaSilvaA.1,CardosoC.2,MechaE1,VazPattoM.C.1,BronzeM.R.1,2,31-InstitutodeTecnologiaQuímicaeBiológica(ITQB),Oeiras,Portugal.2 - Research Institute for Medicines and Pharmaceutical Sciences (iMED), PharmacyFaculty,UniversityofLisbon,Lisbon,Portugal3-InstitutodeBiologiaExperimentaleTecnológica(iBET),Oeiras,PortugalFababean(ViciaFaba,L.) isoneofthemostancientcropsextensivelycultivated nowadays. These grain legumes are rich in carbohydrates,proteins, fat andhave significant amountsof phytochemicals, such as(poly)phenols known for their beneficial health benefits, namely incardiovascular diseases, type II diabetes and cancer. Faba beans areparticularly rich in flavonoids, as (+)-catechin, (-)-catechin and (-)-epigallocatechin, and hydroxycinnamic acids, such as ferulic and p-coumaricacids.Phenoliccontentevaluationoffababeans isusuallybasedonthefreephenolic fraction. However, in legumes there are some phenolics notdirectlyextractable,astheyarepartofcellwalls,consideredasdietaryfibres, due to the rich content in polysaccharides. As conjugated andboundphenoliccompoundsmaybebioavailableafteringestionoffababeans in humans, in order to study the soluble-conjugated and thebound phenolic compounds fractions, a 50% ethanolic extract waspreparedfromafababeangenotype.Theextractandtheresidueweresubmitted to acidic and alkaline hydrolyses and the total phenoliccontentofthe(1)soluble-free,(2)soluble-conjugatedand(3)insolublefractionsweredeterminedbyFolin-CiocalteaumethodCharacterizationof the main phenolic compounds was performed by LC-MS. Theantioxidant activity of these fractions was also determined by ORACmethod. Results are discussed in terms of bioaccessibility and healthbenefitsoffababeanconsumption.

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09:15-09:30 Oral–S7Useofnarrow-leafedlupinb-conglutinproteinsinhumanfoodtotacklediabetesthroughmodulationoftheinsulinpathwayLima-CabelloE.1,AlcheV.2,Robles-BolivarP.1,AlcheJ.D.1,Jimenez-LopezJ.C.1,31 - National Council for Scientific Research (CSIC); 2Estacion Experimental del Zaidin;DepartmentofBiochemistry,CellandMolecularBiologyofPlantsLupinisaworldwideimportantPULSEwithawiderangeofagriculturaland health benefits (nutraceutical properties), i.e. prevention ofcardiovascular diseases, obesity, dyslipidemia. Sweet lupines seem tobe particularly promising as innovative food ingredients and as animportantsourceofproteinsforhumans.Theaimofthecurrentstudywastoevaluatethepotential interactionbetween human insulin and b-conglutin proteins from narrow-leafedlupin(NLL),andtoassessthemodulatoryresponseofgenesinvolvedintheinsulinpathway,andtheinflammatoryresponseintype2diabetes(T2D)patientstosuchconglutinproteins.Ourresultsindicatethatparticularvariantsofb-conglutinsup-regulateby ~2.5-fold the levels of mRNAs from insulin-activation meditatedkinase genes and increase protein synthesis of several gene products(IRS-1/P38/AKT/GLUT-4) in the insulinpathway,possibly through theirinteraction with insulin. Interestingly, various b-conglutins promotedown-regulatory effect in pro-inflammatory genes (IL-1b, iNOS),suggesting the potential therapeutic use of NLL proteins from the b-conglutin family in the prevention and treatment of T2D and otherinsulin-resistantconditions,andthepotential roleof theseproteins intheregulationofinflammatoryprocesses.Acknowledgements:EUMarieCurie grant ref. PIOF-GA-2011-301550; the SpanishMINECOgrantref.RYC-2014-16536;and“JuntadeAndalucía”fortheERDFgrantref.P2011-CVI-7487.

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09:30-09:45 Oral–S7GeneticdiversityinpeaanditsimpactonstrategiesforseedqualityimprovementDomoneyC.1,RaynerT1,MoreauC.1,AmbroseM1,ClementeA.1,IsaacP.G.31-JohnInnesCentre,NorwichResearchPark,UK2-EstaciónExperimentaldelZaidín,Granada,Spain3-IDnaGeneticsLtd,NorwichResearchPark,NorwichNR47UH,UKConsiderable genetic variation exists inPisum sativum L. (pea),whichhas potential for trait improvement but remains under-utilised inbreeding programmes. Our fundamental research on seed quality inpea identifiesallelicvariation ingenesthatcontrolsomeeconomicallyimportant traits, and is providing tools and resources, whichmay beexploitedultimatelywithinbreedingprogrammestoimproveenduses.Naturalgermplasmandinducedmutantpopulationsofpeaareequallyvaluable resources for isolating variants of genes involved indeterminingseedquality.High-throughput screening methods have been deployed to identifyinduced mutations and natural variation in genes that control seedcompositional and visual traits. Although reduced seed trypsin-chymotrypsininhibitormutantswererecoveredamonginducedmutantpopulations, a high-throughput germplasm screen identified anextremely rare nullmutant, aPisum elatius accession,where both ofthecloselylinkedgenesthatencodethemajorseedinhibitorsshoweddeletion of coding sequence. Combining this variant with null seedlectin and pea albumin 2 mutants provides opportunities forconsiderablegaininnutritionalqualityinpeaseeds.Visualtraitscaninfluenceeconomicvalueforfoodcrops.Lossofcolorfrom seeds may be reduced by disrupting the regulation of thechlorophyll degradation pathway, while avoiding perturbations inchlorophyllturnover,whichcandiminishyield.

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09:45-10:00 Oral–S7Understandthestructuringofwheat-legumecakestopromoteproductinnovationandtodesignnewformulationtoolsfortheindustryMonnetA.F.1,2,Jeuffroy,M.-H.2,Michon,C.11 - UMR Ingénierie Procédés Aliments, AgroParisTech, INRA, Université Paris-Saclay,91300Massy,France.2 - UMR Agronomie, INRA, AgroParisTech, Université Paris-Saclay, 78850 Thiverval-Grignon,France.Theagronomicalandnutritionalbenefitsofcereal-legumeassociationsare promising to improve the sustainability of wheat and legumessupplychains.Innovatebygrowingandprocessingbothgrainstogetherwould enhance commercial outlets and thus legume production.Pastries and cakes are mass-market products (0.7% of total foodproductionatEuropeanscale)inwhichlegumes-wheatmixflourcouldbeused.Ouraimwastodevelopareverseengineeringapproachbasedon the understanding of cake structure formation at different lengthscales.Suchapproachwouldallowthedesignofnewproductsandtheproposalofpracticalformulationtoolsfortheprocessingindustry.Theinfluenceofthepartialreplacementofwheatflourbypeaflouronthestructurepropertiesofsoftcakesmadeunderindustrial-likeconditionswas investigated. The batters containing pea-wheat mix flours (P-W)were less dense then control ones (W). However, the air gainedwaslost during baking and the P-W cakes had higher densities than Wcakes.However, thehoneycombstructureofP-Wcakeswasfinerandmore homogeneous thanW cakes.Moreover, the P-W cakes aeratedcakecrumbwassofterthanWcakeonethatisaqualitycriteria.Theseresults are discussed versus pea and wheat flours characteristics(damagedstarch,proteincontentandquality)andversustheevolutionofthedoughduringthedifferentprocesssteps.Thefirstparametersoftheformulationtoolareproposed.

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PostersP43–S7 SustainablesanitationmethodsforkeepingoverallqualityofminimallyprocessedfavaseedsColladoE.1,Artés-HernándezF.1,2,ArtésF.1,2,AguayoE.1,2,FernándezJ.A.1,3,GómezP.A.11-InstituteofPlantBiotechnology.UniversidadPolitécnicadeCartagena.CampusMuralladelMar.30202,Cartagena(Murcia),Spain;2 - Postharvest and Refrigeration Group. Department of Food Engineering, UniversidadPolitécnicadeCartagena.PaseoAlfonsoXIII,48,30203,Cartagena(Murcia),Spain.3-DepartmentofHorticulture.UniversidadPolitécnicadeCartagena,PaseoAlfonsoXIII,48,30203,Cartagena(Murcia),SpainFava beans (Vicia faba) are high in proteins and rich in energy withimportanthealthpromotingcompounds.Duetotheirthickpeel,wholeimmaturepodscannotbeeaten.Freshseedsarehighlyperishablebeingproper sanitation and modified atmosphere packaging (MAP) veryimportant.Inthisstudy,freshseedsfromtenderpods(cv.Palenca)wereimmersedinchlorine (4ºC,150ppm,pH6.5)or,alternatively, inanascorbicacid(4ºC,0.05molL-1pH2.75)orinalemonjuice(4ºC,30ml100mL-1,pH2.5)solution.Seeds(125g)werepackagedin20x15cmOPP35µm-thickbags.Then,bagswerestoredat1ºand4ºC.After10days,atmosphereswithinpackageswere5kPaCO2/16kPaO2and6kPaCO2/15kPaO2at1and4°C, respectively forchlorineand4kPaCO2/17kPaO2forascorbicacidandlemonjuice,independentlyonthe temperature.Microbial growth was higher for seeds treated withascorbicacid(2.3and1.1logCFUg-1usingchlorine,2.4and1.5logCFUg-1forthoseseedsimmersedinlemonjuice,and3.0and3.1logCFUg-1with ascorbic acid, for mesophilics and enterobacteria at 1ºC,respectively).Moreover, growth was about 1 log CFU g-1 lower at 1ºthan at 4ºC. Sensory quality and colour were acceptable until day 7.Then, browning was the main defect, without differences betweentreatments. Data presented here indicate that fava seed can beMAPstoredattemperaturesbetween1ºto4ºCforoneweek.Moreresearchisneededforavoidingbrowningandextendingshelflife.

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P44–S7 ComprehensiveevaluationoffieldpealineswithintheframeworkoftheFP7projectLEGATONaritsL.EstonianCropResearchInstituteA wide-range testing of new breeding materials is an essential toolsource for the knowledge of breeders. The overall aim of the project""LEGumesfortheAgricultureofTOmorrow""(LEGATO)istocontributeto the increased sustainable reintroduction of grain legumes inEuropean cropping systems. The project focuses on identification andtestingofnovellegumebreedinglinesofmajorgrainlegumescultivatedin Europe possessing valuable characters such as disease and pestresistanceandqualityforhumanconsumption.Twelve field pea varieties and seven breeding lines from six Europeancountries were tested in field trials in Estonia. Evaluation ofmorphological and economical characters, resistance for biotic andabiotic stresses and quality was carried out. According to the results,two breeding lines are comperable to better varieties holding thepotentialforthefuture.Workwithlinesiscontinuing.Acknowledgements:TheprojectLEGATO,Grantno613551.

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P45–S7 AnnualvetchesandgrasspeaassuitablecropsfordrylandsofIranAlizadehK.1,KumarS.21-DrylandAgriculturalResearchInstitute(DARI),Maragheh,Iran2-InternationalCenterforAgriculturalResearchintheDryAreas(ICARDA),Rabat,MoroccoStudies on some Vicia species and Lathyrus in different agro-climaticzones of the country were conducted in the Dryland AgriculturalResearch Institute (DARI). Selection of suitable vetch species and theirvarietiesforhayproductionindifferentagro-ecologicalzonesalongwithsoil conservation practices was a critical decision in the forage cropsestablishments in the rainfed conditions. It is intended to increase theproductivityandstabilityofrainfedforagecropstoensurefoodsecurityand improverural livelihoods indrylandareas.This report recommendstake advantage of annual vetch as well as grasspea crops in wheatrotation to overcome different forms of land degradation in drylandareas. This can contribute to improve the capability base of naturalresources,andenhancetheproductivityandsustainabilityoftheexistingagriculturalsystems.Considerablegeneticvariationexistsforforageandgrain yields in the germplasm of vetches (Vicia spp.) and grass pea(Lathyrus spp.) under different environments. In general, grass pea issuitable fordrierenvironmentswhereasvetches suchasViciapanonicaand Vicia villosa are more suitable for cold highlands because of theirabilitytotoleratecoldstress.

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P46–S7 ChoosingtherighttimetoharvestwholecropfababeanKuoppalaK.1,ManniK.2,KänkänenH.1,RinneM.11-NaturalResourcesInstituteFinland(Luke),FI-31600Jokioinen,Finland.2-HämeUniversityofAppliedSciences,FI-31310Mustiala,Finland.ProducingwholecropsilagefromgrainlegumesprovidesanopportunitytoutilizethemmoreefficientlyforruminantsunderNorthernEuropeanconditions where the short length of the growing season limits theripeningoftheseeds.Threecultivarsoffababean(Viciafaba),Fanfare,Honey and Pyramid, were grown in Tammela, Finland (N 60°) as arandomized plot experiment with four replicates. They were sown atJune2,2015.Nofertilizationorplantprotectionwasused.Theherbagesamples were taken biweekly from July 28 to September 22. Thechemical composition and the concentration of digestible organicmatter (DOM) of the samples were analyzed by NIRS. Morphologicalanalysiswasmadebymanually separating the leaves, stemsandpodsaftersampling.Averagedrymatter(DM)yieldincreasedfrom3150to12800kgDM/ha(mean 147 kg DM/d) during the sampling period. The proportion ofleavesandstemsdecreasedandthatofpodsincreasedwithprogressinggrowth. Largest changes occurred during the fastest growth betweentwofirstsamplings,whenaverageDOMdecreasedfrom715to598g/kgDM (8.9 g/day). After that DOM remained relatively stable under 600g/kg DM. Postponing harvest increased the DM yield until the lateautumnwithlittleeffectonDOMbutharvestingconditionswerebetterearlier.Thisworkwasdoneintheproject“ProteinKnowledgeCentreprovidessolutions for protein self-sufficiency in Häme Region” also known asProteinForum.

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P47–S7 Investigationsoncrudeproteinandaminoacidsinorganicallyproducedfieldpea(PisumsativumL.)varietiesAulrichK.,BöhmH.,WittenS.Thünen InstituteofOrganicFarming,FederalResearch Institute forRuralAreas,ForestryandFisheriesThe content of essential amino acids in grain legumes is of specialinterest formonogastric animal feeding in organic farming. Therefore,weinvestigatedtheinfluenceofvarietyonthecontentsofcrudeprotein(CP)andaminoacids(AA)aswellastherelationbetweenCPandAAinfieldpeas.Samplesof73peasof5varieties(Alvesta,Auckland,KWSLaManscha,Navarro, Salamanca) were collected from field trials of organicallymanagedexperimentalsitesinGermanyin2011-2013.ThecontentsofCP andAAwere analyzedwithNIRS. Pearson correlation analyses andmixedprocedureswere conducted in SAS9.4.Variety affectedCP andAAinpeas.AlvestahadthelowestcontentofCP(21%)andalmostallAAperkgdrymatter (p<0.001).KWSLaManschahadthehighestcontentofCP (23%)andallAAper kgdrymatter (p<0.001).However, KWS LaManscha had the lowest amount of total AA and each AA exceptarginineper 100gCP. Therewas a highnegative relationship betweenCP and most of the AA in the CP of field peas (r < -0.63***). Onlyarginine was positively correlated with CP (r=0.61***), while histidineandleucinewerenotrelatedwithCPcontent.Tryptophanandthenon-essentialAAglutamicacidwerelessstronglynegativelycorrelatedwithCP(r=-0.49***).TheresultsindicatethathigherCPcontentscouldleadtomorenon-protein-nitrogenandanalteredrelationbetweenAA.Thus,attention should be paid to amino acids rather than on CP in peavarietiesusedasfeedstuffs.

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P48–S7 ForagequalityandyieldsofsomeperenniallegumesKnotovaD.1,PelikanJ.1,SkladankaJ.21-AgriculturalResearch,Ltd.,CzechRepublic.2-MendelUniversityinBrno,CzechRepublicIn two harvest years two Czech varieties of alfalfa (Holyna andTereza), two varieties of red clover (Spurt and Amos), Hungarianclover (variety Panon) and goat’s rue, Lithuanian variety Gale weretested.Theexperimentwasestablishedbythemethodofrandomizedblocks in four replications. The yields of both green and drymatterwere acquired in all evaluated species. Some quality indicators (%content of ash, crude protein, fibre, ADF, NDF, fat, sugars andnitrogen)weredeterminedfromthebiomassobtainedinthefirstcut.Mycotoxincontentintheharvestedbiomasswasalsodetermined.

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P49–S7 Theincidenceofyeastsandfungiinthelegumes’feedingstuffsfordairycows.KonosonokaI.H.,ProskinaL.,OsmaneB.,CerinaS.LatviaUniversityofAgriculture,LatviaIn Latvia grown peas and beans meal, conventional feedstuffs andimported protein rich feed included in dairy cows’ feeding trialcontrolgrouprationweremicologicallyevaluated.Intotal42samplesof different dairy cows’ feeding stuffs were analysed. All thesesamplesweretestedfortotalcountofyeastsandfungi,presenceandcountofAspergillus spp. andFusarium spp. The assessmentof peameal,beanmeal,soybeanmeal,rapecake,mixtureofpeaandbeanmeal,fodder,andhayontheyeastloadshowedthelowestcountsinpea(2.81log10cfug-1)andbean(3.22log10cfug-1)mealsamples,whilstthehighestcorrespondedtohay(6.99 log10cfug-1)samples.The results obtained on the evaluation of the fungi load of feedingstuffs samples showed that the lowest fungi load corresponded torape cake samples. Bean meal, pea meal, and mixture of pea andbeanmeal samples were contaminated with microscopic fungi in asimilar low degree, 3.28, 3.53 and 3.74, accordingly. The highestcountoffungiwasobservedinhay(5.99log10cfug-1)samples.Fungifrom thegenera Fusariumwerenot found in the feedstuff samples.Aspergillus flavus andAspergillus fumigatus were isolated from thepeasandbeansmealsamplesinrelativelylownumbers.

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P50–S7 ThephysicochemicalpropertiesofextrudedricebasedsnacksfortifiedwithbeanandcarobbeanPedrosaM.M.1,ArribasC.1,BurbanoC.1,MuzquizM.1,CuadradoC.1,CabellosB.1,GuillamónE.2.1 - Food TechnologyDepartment, SGIT-INIA, Ctra de La Coruña, Km 7.5. 28040Madrid,Spain.mmartin@inia.es2-CentrefortheFoodQuality,INA,CampusUniversitarioDuquesdeSoria,42004Soria,SpainConsumers are increasinglyworriedabout thequalityof the food thatingestand lookforamajordiversitythatcan improvetheirhealthandreduce the risk of suffering certain diseases. So, the development offunctionalfoodisanareaofgreatinterestandrepresentsapermanentchallengeforthefoodindustry.FAOandFDAconsiderbreakfast’scerealorsnacksaneffectivewayforthe incorporationofnutrients.Ricehasbeenused inawidevarietyofextrudedproductsduetotheirstarchcontentsince it is responsibleofthehighexpansion rates and themechanical propertiesof extrudates.The inclusion of legumes in their formulation increase nutrient anddietary fibre content, but produces important changes in thephysicochemical properties of the extrudates. This change must beevaluated in order to optimize production parameters and to obtainproductswithoptimumphysicalandsensorycharacteristics.In this study, extrudates with different proportions of rice, bean andcarobbeanweredeveloped.The incorporationof legumesproducedasignificant increase in the density and a reduction of the expansionindex.Thehighestmodificationscorrespondedtotheinclusionofcarobbean flours. Thepastingproperties, textureand colour valuesarealsosignificantlyaffected,sinceareductioninthestarchcontenttakesplacein the flour mixtures, together an increase in the protein and fibrecontent.ThisstudywassupportedbytheProjectRTA2012-00042-C02.

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P51–S7 Nutritionalandnutraceuticalcharacterizationofextrudedgluten-freesnacksmadefromrice,beanandcarobbeanflourmixtures.ArribasC.1,GuillamónE.2,BurbanoC.1,MuzquizM.1,CuadradoC.1.CabellosB.1,PedrosaM.M.11 - Food TechnologyDepartment, SGIT-INIA, Ctra de La Coruña, Km 7.5. 28040Madrid,Spain,arribas.claudia@inia.es2-CentrefortheFoodQuality,INA,CampusUniversitarioDuquesdeSoria,42004Soria,Spain.Consumersarebecomingawareoftheimportanceofdietinrelationtohealth problems, but a large proportion of the population lacks anadequate intakeof fruits and vegetables. Legumes, such as beans andcarobbean,arecurrentlyconsideredgluten-freefunctional foodssincethey are a rich source of nutritional and bioactive compounds withhealth-promotingactivity.Breakfastcerealsandsnacksarethemajorconveniencefoodsproducedbyextrusionandtheyareanintegralpartofthedailyfoodintakeofthemajority of the world’s population. One of the strategies to reduceenergy density of foods is to increase their content of dietary fiber.Takingintoaccountthenutrientprofileoflegumesandmainly,thehighfibre content, they seem to be ideal for inclusion into new foodformulations.Inthisstudy,ithasbeendevelopedandanalysedtheextrudedproductcontaining different proportions of rice (50-80%), bean (20-40%), andcarobbean(5-10%). The incorporationofpulsesproducedan increaseintheprotein,fat,carbohydratesanddietaryfibrecontentsintheflourmixtures.Afterextrusionasignificantreductionwasobservedinthefatand dietary fibre content. The protein content only showed slightvariations,whereasinvitroproteindigestibilitywasimproved.Proteaseinhibitors were abolished after extrusion and inositol phosphatescontentshowedreductionshigherthana15%.ThisstudywassupportedbytheProjectRTA2012-00042-C02.

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P52–S7 Phenoliccontentandantioxidantactivityingluten-freeextrudedcompositefloursofriceanddifferentlegumesGuillamónE.1,ArribasC.2,BurbanoC.2,MuzquizM.2,CuadradoC.2,CabellosB.2andPedrosaM.M.2.1-Centre for theFoodQuality, INA,CampusUniversitarioDuquesdeSoria,42004Soria,Spain,guillamon.eva@inia.es.2 - Food TechnologyDepartment, SGIT-INIA, Ctra de La Coruña, Km 7.5. 28040Madrid,SpainTheincorporationofplantfoodstothehabitualdiet isthelatesttrendinahealth strategy topreventand treatdiseases.Cereals,particularlyrice, and legumes are the most consumed plant food in the world.Fortifiedcerealflourwithlegumesprovidestheopportunitytodevelopnew foods for increasing their nutritional value and incorporatingbioactive compounds. Among the physiological active substancespresent in legumes, phenolic compounds have attracted muchattention. Their mechanisms of action are commonly related to theirexcellent antioxidant properties. The extrusion/cooking is an efficientprocessingtechniquetoobtainnewfood,withaclear improvementoftheirtextureandflavor.Theaimofthisstudywastoevaluatetheeffectofextrusionofflourscontainingdifferentportionofrice(50-80%),beanorpea(20-40%),andcarobbean(5-10%)ontotalphenols,anthocyanin,flavonol and tartaric ester contents and their antioxidant activity(ORAC).Theincorporationoflegumes,particularlypeaandcarobbean,producedanincreaseintotalphenolsandsomeofphenoliccompounds,as well as in antioxidant activity. Extrusion affected to antioxidantactivityandcontentofsomephenoliccompounds.Asignificantpositivecorrelation between the antioxidant activity and the total phenols or,someofthephenoliccompoundswasobserved.ThisstudywassupportedbytheProjectRTA2012-00042-C02.

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P53–S7 Studyofimbibitionpropertiesandcompositionoflegumesfloursinrelationtoitstechnologicalcompetence.Case:foodgluten-freebakedLopera-CardonaS.,AlvarezR.C.,GallardoC.,SalamancaR.,YanezS.,delaHozS.Group of the Stability of Medicines, Cosmetics and Food, Fac. of PharmaceuticalChemistry,UniversidaddeAntioquia,Calle67Nº53-108,Bloque2,Medellín,Colombia.seneida.lopera@udea.edu.coLegumes are characterized by their endosymbiosis with nitrogen fixingorganisms,becominga richsourceofprotein.Theaimwas tostudypulsesflours of Pisum sativum, Cicer arietinum and Lens culinaris, and Waterabsorptioncapacityandminimumgelationconcentration,usingmethodsofthe AOAC The protein profile by Osborne and Bradford methods. Glutenwere determined according CODEX STAN 118. Product prototypes weredeveloped,withflouroflentil,evaluatedthesvolumeaccordingtotheAACCmethod. The results showed that the protein content was higher than26.6±2%,beingandthelipidcontentwasthelowerinallflours,3.1±0.4%.Ingerminatedflourlentil,theMGCdecreasesandWACincreases,inrelationtoflourungerminatedthatcouldberelatedtoefficiency ingel formationandstructure.Thealbuminproteinfraction,containedinallfloursonaverageof19.25±4µg/µL,byenzymesandproteinsofhigherdigestibilityaccordingtostudiesreviewed.Chickpeaflourshowedthehighestvalueof44.39±2µg/µLglobulins,asstorageproteins.Allfloursshowedprolaminasandglutenlowerthan12.63ppm,inthisorder;lentilgerminated>chickpea>pea>Lentil,lessthan20ppm, suitable tobe labeledasgluten-free.Productprototypesareperceivedwithasofttouch,anapparentdarkercolorandspecificvolumeof2.21cm3/g, similar to the control of wheat. As a general conclusion, thefindings present an opportunity for legumes in diversification ofconsumption.

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P54–S7 IntercroppingsainfoinwithannualcropsforforageproductionandweedcontrolMiloševićB.,KaragićD.,MihailovićV.,KatanskiS.,MilićD.,VasiljevićS.,ŽivanovD.InstituteofFieldandVegetableCrops,NoviSad,SerbiaSainfoin in combinationwith foragepea, fababeanoroat,providesasuperb feed fordomesticanimalssuchascows, lactatingewes,goats,etc. As well, one of the benefits of this kind of variety mixture isreducingtheweedsinforage.The aim of this study was to examine the potential of intercroppingsainfoinwith annual crops (foragepea, fababeanandoat) for forageproduction. The trial with three replications was carried out atExperimentalFieldofInstituteofFieldandVegetableCropsNoviSadatRimskiAnevi.Sainfoinwastheundersowncrop,andforagepea, fababean and oat were the companion crops. Control variant was purestandofsainfoin.Attheharvestablestageofsainfoin,forageyield(tha-1)andweedproportion(%),weremonitored.Inallreplications,sainfoinmixturewithforagepea,fababeanandoatproducedthehighestgreen forageyieldperhectare incomparison tocontrol.Theaveragegreenforageyieldinmixturewithoat(16.9tha-1)wassignificantlyhigherthaninforagepea(10.2tha-1)andfababean(11.4 t ha-1) mixture. The significantly lowest weed proportion hasbeen achieved in sowing with oat (1.5 %), while pure crop had thehighestweedpercentage(6.4%,respectively).Acknowledgement:ThisresearchwassupportedbyTheLEGATOProject"LEGumesfortheAgricultureofTOmorrow",projectnumber613551,isfundedbytheEuropeanUnionundertheFP7Programme.

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P55–S7 IntercroppinglegumeswithlegumesanditseffectsonyieldcomponentsŽivanovD.,KaragićĐ.,MiloševićB.,VasiljevićS.,ĐorđevićV,SavićA.,MikićA.InstituteofFieldandVegetableCrops,NoviSad,SerbiaTheexperimental trialwasestablished in theexperimental fieldof theIFVCNS, using completely randomized block design with threereplications.Theplotsizewas5m2andtheseedingratioswas50:50%.Pea +faba bean, pea + fenugreek and grass pea + white lupin weremixed together, andmechanically sown in rows at the same depth. Atotaloftenplantsrandomlychosenperplot inthreereplicationswereestimated for the yield components (the number of pods, number ofseedsandseedweightperpod.Theaveragenumberofpods(7.6/8.2),numberofseeds(26.9/30.1)andseed weight (6g/7.2g) per pea plant in a mixture with faba bean andfenugreekwassignificantlyhigherthaninsolecrop,wheretheaveragenumberofpodswas5.2,numberofseeds19.2,andseedweight4.7g.However, inthesamemixturetheaveragenumberofpods(12.3/13.8)and seeds (29.7/57.7) per faba bean and fenugreek plants weresignificantlyhigherthan in intercrop.Moreover, thefenugreekhadthesignificantly higher seedweight (0.9g) inmixtures than in a sole crop.Thesolecropsofgrasspeaandwhitelupinhadthesignificantlyhighernumberofpods(18.5/9.2),seeds(44.1/29)andseedweight(7.25/13.2)theninmixture.Acknowledgement:ThisresearchwassupportedbyTheLEGATOProject"LEGumesfortheAgricultureofTOmorrow",projectnumber613551,isfundedbytheEuropeanUnionundertheFP7Programme

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P56–S7 CharacterizationofsaponinproductionindevelopingseedsofViciafabaandPisumsativumMaraisJ.1,VernoudV.1,LeSignorC.1,Lacaille-DuboisM-A.2,ThompsonR.11-INRA,UMR1347Agroécologie,INRACentredeDijon,RueSully,F21000DIJON,France2 - Université de Bourgogne-Franche Comte, EA 4267, FDE/UFC, Laboratoire dePharmacognosie,FacultédePharmacie,F21079DIJON,FranceLegume seeds are increasingly in demand as an inexpensive source ofprotein for incorporation in foodstuffs. However, protein fractionsprepared from pea (Pisum sativum) and faba bean (Vicia faba) seedscontain significant quantities of saponins, which may confer anundesired bitter flavor on the final food product. Saponins have alsobeen implicated in abiotic andbiotic stress responses,hencea furtherinterest in studying their accumulation and genetic control in thesespecies. We have identified pea genes putatively involved in saponinbiosynthesis in developing pea seeds, and optimized an extractionmethod for use in following saponin accumulation and distribution inseedsofpeaandfababean.Acknowledgement.Thisproject is supportedby theFUI (FondsUniqueInterministériel)AAPno.18LEG’UP.

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P57–S7 IntercroppingfieldpeawithperenniallegumesforforageproductionKatanskiS.,MilićD.,KaragićD.,MiloševićB.,VasiljevićS.,MikićA.,MihailovićV.InstituteofFieldandVegetableCrops,NoviSad,SerbiaOneofthemost importantproblems inestablishingperennial legumesare weeds in the stages of the early growth and development of aperenniallegume.Fieldpeaisashortterm,annualcropandcanreduceweedinvasion,andalsoitcanprovideincreasedforageyieldinthefirstcutinthefirstyear.Thegoalofthisstudywastoexaminethepotentialofintercroppingfieldpeawithperenniallegumesforforageproduction.ThetrialwiththreereplicationswascarriedoutatExperimentalFieldofInstituteofFieldandVegetableCropsNoviSadatRimskian_eviin2015.Alfalfaandredcloverweretheundersowncrops,andfieldpeawasthecompanion crop. Control variants were pure stand of alfalfa and redclover and their mixture with oat. At the harvestable stage of pea,forage yield (t ha-1) and weed proportion (%) were monitored. Onaverage,intercroppingwithoatproducedthehighestgreenforageyield(22.6tha-1alfalfaand18.9tha-1redclover).Forageyieldwashigherwhen perennial legumeswere sownwith pea than grown as the purecrops (10.0 tha-1vs.6.4 tha-1,10.8 tha-1vs.8.7 tha-1).The lowestweedproportionhasbeenachievedinsowingwithoat(0.4%alfalfaand0.3%redclover),whilepurecropshadthehighestweedpercentage(13%and46%,respectively).AcknowledgementThisresearchwassupportedbyTheLEGATOProject"LEGumesfortheAgricultureofTOmorrow",projectnumber613551,isfundedbytheEuropeanUnionundertheFP7Programme.

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P58–S7 Applicationoflegumesindevelopmentofcrunchyprotein-richsnackbars.GaloburdaR.,PuiskinaE.,Muizniece-BrasavaS.LatviaUniversityofAgricultureEnergy, cereal or chocolate bars available in themarket typically havehighcarbohydrate(54–70g,onaverage65gper100gofproduct)andfatcontent(7–23gper100gofproduct).Theaimofthecurrentstudywastodevelopcrunchylegumebarswithincreasedproteinandreducedcarbohydrate and fat content. Extruded snacks produced from Latviangreypeas (Pisumsativum L.)of thevariety ‘Bruno’,broadbeans (Viciafaba L.)of thevariety ‘Barteks,’andcowpeas (Vignaunguiculata L.)ofthevariety‘Fradel’fromPortugalwereusedasabasisfornewproductdevelopment. After sensory evaluation, the highest consumeracceptanceamongdevelopedproductsreceivedsnackbarsmadefromgrey peas, which were selected for further study. Suitability of thefollowing packaging materials was evaluated – polypropylene (PP),metalizedpolypropylene(metPP),andpolylactide(PLA).Studyrevealeda small change in colour of the product during 90 days of storage atroom temperature; the product became darker. The least changes inproduct color were observed in metPP packaging. Moisture contentdecreased, but product hardness increased, in a close correlation(r=0.98). The pH during storage did not change significantly. Wateractivity (aw) in the new crunchy protein-rich snack barswas less than0.49.Microbialsafetyofproductwasprovedbymicrobiologicalanalysesperformedduringitsstorage.Nutritionalandenergyvalueofpeasnackbarwasexperimentallydetected.

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P59–S7Biomassproductioninanimprovedsustainablemixedshort-rotationwoodycroppingofPopulushybridsandRobiniapseudoacaciaRebola-LichtenbergJ.,AmmerC.UniversityofGöttingenTherisingdemandonbionenergyleadstoanecessityforoptimizationof biomass production. Hardwood short-rotation coppice crops areusedasasourceforcarbon-neutralenergy.Mixedcropping isseenasan improvementtowardshigherecologicalcomplexity. Intercropping -the cultivationof different cropson the same landat the same time-may lower the dependence on additional input by recovering theinternal regulation of a natural ecosystem and enhancing the cropsystemsstability.Due to their rapid high woody biomass production the fast-growingPopulus hybrids are particularly used as elements for bioenergyproduction in monocropping systems. Robinia pseudoacacia L. is alesser known species for energy plantations, but has the promisingabilitytofixnitrogen.This study aims to throw light on a potential facilitation andcomplementarity between the N-demanding species poplar (Populusspp.) and the N-fixing legume species black locust (Robiniapseudoacacia) onbiomass increment.Alongwith treemeasurements,the crown structureand leaf-area-indexwill beanalyzedas indicationfortreeinteraction.Thepoplarhybridsthatbenefitmostfromthistypeofcropmixturewillbeidentified.

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P60–S7 RelationshipbetweenphysicochemicalcomponentsKarayelR.1,BozogluH.21-BlackseaAgriculturalResearchInstitute,Samsun2-OndokuzMayısUniversity,FacultyofAgriculture,AgronomyDepartment,SamsunThisstudywasconductedtodeterminerelationships inbetweensomephysicochemicalpropertiesof4controlvarietiesand44 localpea lineswhich has been collected from Blacksea Regions and obtained fromGene Bank of Eagean Agriculture Research Institute. Relationshipbetweenpropertieswasdeterminedbycorrelationandpathanalysis.Itwas determined that cooking time was very significantly and positivecorrelatedwithwaterabsorptioncapacity,swellingcapacity,drymatterrate passed to cooking water, seed length, protein rate in seed,tryptophan quantity in seed, ash rate in seed and P quantity in seed;significantlyandpositivecorrelatedwith100seedweight,amyloseratein seed and Ca quantity in seed; very significantly and negativecorrelated with fragmentation ratio of seed after cooking and starchrate inseed. Itwasdeterminedthatdrymatterratepassedtocookingwater(0.5637)andwaterabsorptioncapacity(0.5459)havepositiveandhighest direct effects; starch rate in seed (-0.3548) and K quantity inseed(-0.2900)havenegativeandhighestdirecteffectsoncookingtimeintheandofpathanalyses.Starchrate inseedhaveeffectednegativeto cooking time (0.641**) in other words the increaser starch rate inseed, the shorter cooking time. According to the path analysis, directeffects of starch rate in seed to cooking time is% 28.4736 and itwasseeing the property doing highest indirect effect is water absorptioncapacity(%16.9914).

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P61–S7 Micropropagationofforagelegumeelitegenotypes:solutionsandprospectsDuqueA.S.1,CostaO.C.1,2,MedalhasM.2,SilvaA.R.2,AraújoS.S.1,BarradasA.2,CrespoJ.P.2,FevereiroP.1,31 -PlantCellBiotechnologyLaboratory,Green-itUnit, InstitutodeTecnologiaQuímicaeBiológicaAntónioXavier(ITQB),Apt.127,2781-901Oeiras,Portugal;2-Fertiprado-SementeseNutrientesLda.,7450-250Vaiamonte,Portugal;3 -DepartamentodeBiologiaVegetal,FaculdadedeCiênciasdaUniversidadedeLisboa,CampoGrande,1749-016Lisboa,PortugalThere is an ever-increasing demand for high quality forage legumes,essentialtofeedlivestock.Micropropagationmaybeusefultopreserveeliteallelic compositions inallogamousandauto-incompatible species.Forclonalmultiplicationofelitegenotypesmicropropagationprocessesfor Trifolium incarnatum, T. resupinatum, T. michelianum, T.isthmocarpum, T. alexandrinum and Hedysarum coronarium weredeveloped. Axillary shoot development was induced by cultivatingdisinfected nodal segments on agar solidified MS basal medium,supplemented with sucrose and 0.0-1.8µM benzyladenine.Micropropagated shoots were rooted onMSmediumwithout growthregulatorsand80to100%weresuccessfullyacclimated.Averyproneto etiolate T. resupinatum genotype stood out by presenting anextremely etiolated-like phenotype with long, weak stems and sparseleaves,togetherwithhighpercentagesof invitroflowering.Toreversethe etiolation like effect and flowering induced by in vitro culture,zeatin,benzyladenineandgibberellicacidsupplementationwastested.Supplementation with 9.12µM zeatin reversed the etiolation-likephenotype,inhibitedinvitrofloweringandimprovedacclimationinthisT. resupinatum genotype. To investigate the photosyntheticperformanceofinvitropropagatedT.resupinatumelitegenotypes,gas-exchange measurement coupled to pigment quantifications are alsobeingconductedatFertipradotrialfieldsandgreenhouse.

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P62–S7IntercroppingofPeaandCerealsinOrganicFarmingHunadyI.,HochmanM.AgritecPlantResearchLtd.Thelegume-cerealintercropping(LCI)maybeadvantageoustoincreaseself-sufficiency with animal fodder in organic farming and reduce thepossible dependency on imported soybeans and extracted soy meal.Finally, the reintroduction of LCI growing asmajor crop appears to bevery promising way to stabilize yields, decrease weed and pestspressure.Fieldexperimentswithplot trialswith intercropped leafyandafilapeavarieties and spring cereals (wheat, barley) were carried out in theCzech Republic over four consecutive cropping seasons (2008–2011).Different varieties and pea-cereal combinations were tested to findusefulvarietiesforintercroppingandoptimalpeatocerealratio(peatocereal ratios 80:20, 60:40, 40:60, 20:80). The results demonstrate thatpea-cereal intercrops compared to sole crops produce high yields ofgrainsespeciallywhen intercroppedat thepeatocereal ratioof60:40and 40:60. To determine land use efficiency, the land equivalent ratio(LER) was evaluated. It emerged that LER values must be interpretedcarefullyandshouldnotbeusedwhenyieldlevelsofintercroppedpea-cerealcomponentsareextraordinarilyloworhigh.

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P63–S7 Wholechickpeaflourandcrudemucilageadditionaffectkefirqualityandantioxidantcapacityduringstorage.OuldsaadiL.1,MahtoutR.1,BoudjouS.1,ZaidiF.1,HassisseneN.1,OomahB.D.2,HosseinianF.31-DepartementdesSciencesAlimentaires,FacultédesSciencesdelaNatureetdelaVie,UniversitédeBejaia,06000Bejaia,Algérie2 - FormerlywithNationalBioproducts andBioprocessesProgram,AgricultureandAgri-FoodCanada,PacificAgri-FoodResearchCentre,Summerland,BC,Canada,V0H1Z03 - Food Science Department of Chemistry Carleton University and Nutrition Canada.nadhassi1@gmail.comThis study investigated the capability and efficiency of the wholechickpeaandhiscrudmucilagetoenhancebacterialsurvivalandgrowthinkefiraswellastheantioxidantactivityofsamples.The results showed that kefir samples supplemented with flour andmucilage enhanced significantly (P < 0.05) higher viability of bacteria(8.36logcfu/mL)andacidity(0.82%TTA)thanthecontrol.AnoverallpHdecline of 0.2was observed during cold storage period. Themucilagefromcrudechickpeaexhibitedstrongantioxidantactivity;ORACvalueof267.45±3.3_molTroloxequivalent/g,inagreementwithhighTPC.Thisstudydemonstratedthatchickpeaflourandhismucilageactsasagoodsourceofprebiotic,enhancinglacticacidbacteriagrowthinkefirmodelinadditionoftheirhighantioxidantbeneficiary.

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P64–S7 Usesoffababeansandchickpeasinfood(pasta,breadandkefir)OuazibM.1,TazrartK.1,MoussouN.1,BoudjouS.1,OuldsaadiL.1,MahtoutR.1,HosseinianF.2,LamacchiaC.3,HarosM.4;GarzonR.5;MRosellC.M.5,HassissenN.1,ZaidiF.11-DépartementdesSciencesAlimentaires,FacultédesSciencesdelaNatureetdelaVie,UniversitédeBejaia,06000Bejaia,Algerie2-FoodScienceDepartmentofChemistryCarletonUniversityandNutritionCanada3-DipartimentodiScienzedegliAlimenti,UniversitàdegliStudidiFoggia,ViaNapoli,25,71100Foggia,Italy4 - Institute of Agrochemistry and Food Technology (IATA-CSIC), Av. AgustínEscardino7ParqueCientífico,46980Paterna-Valencia,Spain5-FoodScienceDepartment,InstituteofAgrochemistryandFoodTechnology(IATA-CSIC),C/AgustinEscardino,7,46980,Paterna,Valencia,Spain.idiazdiraf@yahoo.frPasta, bread and kefir are very popular food in several countriesaround the world, easy to prepare and well accepted by all agegroups.The incorporation of broad-bean flour in maccheronccini pastaresulted in a significant increase in protein levels, fiber, resistantstarch, ash and minerals. In vitro protein digestibility increasedproportionally with the broad-bean substitution level. Lowerglycemicindex(GI)forenrichedpastacomparedtotraditionalpastaandwhitebreadisnoted.Bread made with raw chickpea flour had the highest proximatecomponent content but the lowest in vitro protein digestibility.Finallyaboutsensoryproperties,nosignificant(p<0.05)differenceswere observed in texture, after-taste, aroma and overallacceptability among the different breads. Concerning appearance,breads made with raw and germinated chickpea were moreappreciated,however,breadmadewithcookedchickpeaflour,wastastier than other breads. Processing of chickpea beans could beused for obtaining gluten free breads with good nutritionalcharacteristics.Whole ground faba bean was investigated for its capability andefficiency to enhance bacterial survival and growth during kefirstorage.BacterialcountwasstronglyandpositivelycorrelatedwithlacticacidcontentandinverselyassociatedwithpH.

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P65–S7 Theinfluencelegumesincombinedorganicfertilizersonspringcerealsproductivityandquality.KadžiulienėŽ.,ArlauskienėA.andŠarūnaitėL.LithuanianResearchCentreforAgricultureandForestryLegumes and organic fertilisers are tools for supporting of nutrientcyclinginlow-inputsystems.Yieldbenefitstosubsequentcropsarethemajorcomponentofpre-cropvalueof legumes,howevermanagementof legume is complicatedanduse in crop rotations is insufficient.Alsousing only the nitrogen fixed by legumes, it is not easy to meet thenitrogenneedsofothercropsattherighttime.Itisthereforeimportantto find out measures that may contribute to grain yield and qualityimprovement. The experiments were carried out on Endocalcari-EphypogleyicCambisol inDotnuva(55˚24'N,23˚50'E),andonaheavyloamCambisol at the JoniškėlisExperimental Station (56º21'N,24º10'E). The study aims to combine legumes and newer forms of organicfertiliserascowmanurepelletsandfindouttheeffectonspringcerealsproductivity and quality. Grain yield of spring wheat was affected byusing manure pellets and biomass of incorporated red clover in bothlocations.Cattlemanurepelletsused for cultivationofoatsand springwheathadapositiveimpactonbarleyyield.

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P66–S7 EvaluationofedamameinLatviaZeipinaS.,LepseL.PūreHorticulturalResearcheCentre.LatviaThedifferencebetweensoybeans(Glycinemax)andedamameisinthestage of maturity when the beans are harvested. Vegetable soybeanalso called edamame is an important vegetable. Beans are harvestedbefore full maturity when bean pods are green and just startedbecomes yellow. Edamame is a valuable food for vegetarians andvegansduetoitshighnutritionalvalue.Edamamehas376calories,17grams total fat, 28 grams total carbohydrates per cup. Preliminaryinvestigationswiththeaimtoclarifyedamamegrowingpossibilities inLatvia were carried out in Pūre Horticultural Research Centre Ltd. in2015. Five Japanese cultivars were tested: ‘Sappon Miclin’, ‘SoyaComachi’, Kaohsiung No 9’, ‘Chiba Green’ and ‘Midori Giant’.Meteorological conditions in vegetation period of 2015were suitablefor soya growing. Fresh pod yield ranged between 1.3 – 5.2 t ha-1,higheryieldwasobtainedfor‘MidoriGiant’.Inorganolepticassessmentvariety‘ChibaGreen’showedslightlybetterresultsthanothervarieties.

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Session 8, plenary: Frontiers in legume genetics andgenomics-RoomArrábidaI&IIChairedbyRobertoPapa(Univ.LeMarche,Italy)andMartaSantalla(CSIC,Spain)10:30-11:00 Key lecture - Judith Burstin: Towards thegenomesequenceofpea:atributetoMendel

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OralCommunications11:00-11:15 Oral–S8Genomesequencingofadzukibean(Vignaangularis)providesinsightintohighstarchandlowfataccumulationanddomesticationWanP.BeijingUniversityofAgricultureAdzuki bean (Vigna angularis) is an important legume crop, and isgrown inmore than thirty countries of theworld. The seedof adzukibean, as an important source of starch, digestible protein, mineralelementsandvitamins,iswidelyusedinavarietyoffoodsforatleastabillion people. Here, we generated a high quality draft genomesequenceofadzukibeanbywholegenomesequencing.Theassembledcontig sequences reached to 450 Mb (accounting for 83% of thegenome)withanN50of38kb,andthetotalscaffoldsequenceswere466.7 Mb with an N50 of 1.29 Mb. Of them, 372.9 Mb of scaffoldsequenceswereassignedtothe11chromosomesofadzukibeanusinga single nucleotide polymorphism genetic map. A total of 34,183protein-codinggeneswerepredicted.Functionalanalysisrevealedthatsignificant differences in starch and fat content between adzuki beanand soybean were likely due to transcriptional changes, rather thancopynumbervariations,ofthegenesrelatedtostarchandoilsynthesis.We detected strong selection signals in domestication by thepopulationanalysisof50wildandcultivatedadzukibeans.Thegenomesequence of adzuki bean will facilitate the identification ofagronomically important genes, and accelerate the improvement ofadzukibean.Insummary,ourresultsprovideinsightintoevolutionandmetabolicdifferencesoflegumes.

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11:15-11:30 Oral–S8Genotyping-by-sequencinganditsexploitationinforageandgrainlegumebreeding.AnnicchiaricoP.1,NazzicariN.1,WeiY.2,PecettiL.1,BrummerE.C.21-CREA,Italy2-Univ.ofCalifornia,DavisGenotyping-by-sequencing(GBS),comparedwithSNParrayplatforms,may drastically reduce genotyping costs, and is applicable also torelativelysmallexperiments.However,itmayrequireoptimizationforspecific crops, tomaximize the number of usablemarkers. ExploitingGBS-generated markers may require optimization too (e.g. to copewith missing data). This study aimed (i) to compare the value ofdifferent GBS protocols for three legume crops (alfalfa, pea, whitelupin)thatcontrastforgenomesize,ploidyandbreedingsystem;(ii)toshow various successful applications of GBS data on legume speciesand someof their challenges. Preliminarywork on alfalfa highlightedthe greater interest of ApeKI over PstI:MspI DNA digestion. Wecomparedall different combinationsofKAPAorNEBTaqpolymerasewith primer extensions that are progressively more selective onrestrictionsites, identifyingGBSprotocolsofgeneralvalueacross thethree legume species. We assessed genomic selection accuracy forpredictingforageyield-basedalfalfabreedingvalueandpeagrainyieldindifferentgrowingenvironments,consideringdifferentmissingvalueimputation strategies and statistical models (Ridge regression BLUP,SVR, Bayesian methods). We tested the usefulness of GBS data toassess alfalfa cultivar distinctness, which offers advantages overphenotypic trait-based distinctness. GBS proved useful also forexploringthegeneticstructureofwhitelupinlandracegermplasm.

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11:30-11:45 Oral–S8ApplicationofhistoricaldatafromAustralianlentilbreedingprogramtoenablerapidimplementationofgenomicselectionPembletonL.1,SudheeshS.1,BraichS.1,RoddaM.2,SlaterT.1,ForsterJ.W.1,3,CoganN.O.I.1,3,SpangenbergG.1,3,KaurS.1,*1) AgriBio, Centre for AgriBioscience, Agriculture Victoria, DEDJTR, 5 Ring Road, LaTrobe University, Bundoora, Victoria 3083, Australia.sukhjiwan.kaur@ecodev.vic.gov.au2)GrainsInnovationPark,DEDJTR,PMB260,Horsham,Victoria3401,Australia3)LaTrobeUniversity,Bundoora,Victoria3086,Australia.Genomic selection (GS) has recently emerged as an evolutionaryapproach in cropbreeding that canaccelerategenetic improvementthroughtheabilitytopredictthephenotypicperformanceearlyinthebreedingcycle,reducingthegenerationinterval.Hereweevaluateforthefirst time itsefficacyforbreeding lines fromtheAustralian lentilbreeding program. A total of 864 advanced breeding lines wereevaluatedinfieldannuallyinarangeofenvironmentsfrom2010-2014foreconomicallyimportanttraitssuchasgrainyieldandgrainweight.Genotyping was performed using a whole genome genotyping-by-sequencing transcript approach. Over 200,000 SNPs were initiallyidentified from sequencing key ancestor lines of the PBA breedingprogram,andwereusedforSNPvariantcallinginthebreedinglines.The ability to genomically predict the observed phenotypicperformance in each progressive year, and within-year clusteredenvironmentswasexploredbyusinglinesfrompreviousyears,asthereferencepopulationandapplyinga rangeofGSmodels i.e.GBLUP,BayesA and BayesB. Accuracies achieved for grain yield and weightwere moderate to high (c. 0.35-0.70), reflecting the relativeheritabilitiesofthesetwoeconomicallyimportanttraits.Thegenomicestimated breeding values and prediction equations developed arenowbeingimplementedwithinthelentilbreedingprogramtodesignoptimal crossing schemes to increase genetic gain and reduce theoverallgenerationinterval.

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11:45-12:00 ORAL–S8Legume-basedmixedcroppingsystemsmayhavehigherwateruseefficiencythanmonocropsystemsWGGBSinpeawithoutreferencegenomeanddataassembly:asuccessfulstrategyatlowsequencingcoverageforSNPdiscovery,genotypingandgeneticmapping.BoutetG.1,9,AlvesCarvalhoS.1,PeterlongoP.2,FalqueM.3,LhuillierE.4,6,BouchezO.4,7,LavaudC.1,9,UricaruR.1,8,LesneA.1,9,Pilet-NayelM-L.1,9,RivièreN.5,BarangerA.1,9 1-INRA,UMR1349IGEPP,BP35327,LeRheuCedex35653,France2-INRIARennes-BretagneAtlantique/IRISA,EPIGenScale,Rennes,35042France3-INRA,UMR320GQE–LeMoulon,INRA-UnivParis-Sud-CNRS-AgroParisTech,FermeduMoulon,91190Gif-sur-Yvette,France4-GeT-PlaGe,Genotoul,INRAAuzevilleF31326,Castanet-tolosan,France5-LimagrainEurope,routed’Ennezat,CS90126,Chappes63720,France6-INRA,UAR1209,Castanet-Tolosan,France7-GenPhySE,UniversitédeToulouse,INRA,INPT,INP-ENVT,CastanetTolosan,France8-UniversityofBordeaux,CNRS/LaBRI,F-33405Talence,France9-PISOM,UMTINRA/TerresInovia,BP35327,LeRheuCedex35653,FranceOurobjectivewastodevelopacomprehensiveSNPresourceinaspecieslackingasequencedreferencegenome(Pisumsativum),bydevelopingawhole genome genomic DNA genotyping by sequencing (WGGBS)strategy for high-throughput SNP discovery, genotyping and geneticmapping.Over four hundred thousand SNP markers were identified fromsequencing 4 pea lines (7x coverage), among which 63,353 weresuccessfully genotyped andmapped through genome sequencing of 48RILs (3.5x coverage), without prior genome reduction or assembly to areferencegenome(Boutetetal.,2016).AsubsequentKASP™assayon1438pealines,withasubsetof1000SNPschosen for theirmapping positions within QTL confidence intervals forbiotic stress resistance, showed that almost all generated SNPs werehighly designable and that most (95 %) delivered highly qualitativegenotyping results. It allowed refining map genomic regions and QTLsassociatedwithtraitsofinterestidentifiedfromRILpopulations.Using rather low sequencing coverages and the development andoptimization of appropriate tools such as discoSnp in SNP discovery orcustom R scripts using CARHTA GENE for genetic mapping, we madeavailable a large set of polymorphicmarkers internal to and borderingQTLconfidence intervals.Thisprovidesanunprecedentedresourcethatcouldsignificantlyspeedupgeneticstudiesandmarkerassistedbreedingfornon-modelspecies.Boutetetal.(2016)BMCGenomics17:121

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PostersP67–S8 Validationofquantitativetraitlociforheight-relatedtraitsinfababean.AtienzaS.G.1,Ruiz-RodriguezM.D.2,Cruz-IzquierdoS.2,TorresA.M.2,CuberoJ.I.3,AvilaC.M.2.1-Inst.Agric.Sostenible(CSIC)2-IFAPA-CentroAlamedadelObispo(Córdoba)3-UniversityofCórdobaMost faba bean genotypes show indeterminate growth habit. Thesegenotypesmay become very tallwhenwater is available. Indeed fababeancropsmaygrowtoaheightof2matmaturityalthoughfababeancropsarenotusuallythattall.Excessiveplantheight(PH)mayresultinplant lodging. Besides, the crop may result in poor pod set due topredominantvegetativegrowth,unevenmaturity.Accordingly shorter genotypes are important in faba bean breeding.Otherheight-related traitsarealso important. Theheightof the lowerflower (HLF) and the height of the lower pod (HLP) are importantagronomic traits. Pods located near ground may remain unharvestedandtheymaybemoreaffectedbydiseaseincidence.Previous studies in our group identified preliminary quantitative traitloci(QTL)forthesetraitsinthepopulationderivedfromthecrossVF6xVf27. In thisworkwehave validatedQTL for PH,HLP andHLF at fieldconditions.Besides,theroleofcandidategenesforheight-relatedtraitsinmodelspecieswasinvestigatedinrelationwiththeseQTL.Acknowledgments: This work was financed by the Ministerio deInnovación y Ciencia (MICINN) grant AGL2008-02305 and FEDER.MDRwasrecipientofapre-doctoralFPIfellowshipassociatedtothisresearchgrant.

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P68–S8GenotypingofPisumsativumL.andViciafabaL.bySNPanalysisusingnextgenerationsequencing,Martos-Fuentes,M.;Appeltans,J;Maes,I;Mezaka,I.;Egea-Gilabert,C.;Fernández,J.A.;Egea-Cortines,M.andWeiss,J.ETSIAUniversidadPolitécnicadeCartagenaSNPs (single nucleotide polymorphism) are an important source ofpolymorphisms throughout the genomeand therefore a valuable typeofmarker to study genetic distances.Wehave chosen 45markers forPisumsativumand60forViciafaba,identifiedinQTLmappingprojects.We used Next Generations Sequencing with the IonTorrentSemiconductor Sequencer. We developed a barcoding system thatallowedusefficientamplificationsandsequencingallSNPsfor12plantsfrombothspeciesonasingleIonTorrentchip.ThemethodisbasedontwoconsecutiveconventionalPCRs.Inthefirstone,SNPspecificprimerswere used to amplify the specific region, adding a bead specificsequence (P1) to one end and a common sequence “B” to the otherend.ThesecondPCRwasperformedusingP1asforwardprimerandanadaptor“A”+“B”specificsequenceasreverseprimer.Theadaptor“A”containsthebarcodeof4nucleotides,differentiatingfor12plants.Firstroundsofsequencingshowedthat40ofthe60SNPsinV.fabaand37ofthe 45 SNPs in P. sativum gave reliable sequence results. Some DNAextractions failed to give sequencing reactions. The percentage ofpolymorphic SNPS was 70% both for P. sativum and V. faba and thepercentageofheterozygositywas54%inP.sativumand67%inV.faba.SNPsequence informationofa totalof72varietieswillbe finallyusedfor phylogenetic reconstruction in order to obtain information aboutsimilaritiesatthemolecularlevel.

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P69–S8 UsingGBS-GWAStoIdentifyLociControllingForageYieldandNutritiveValueinaDiploidAlfalfaMappingPanelSakirogluM.1,BrummerE.C.21-DepartmentofBioengineering,KafkasUniversity,Kars,Turkey.2-DepartmentofPlantSciences,TheUniversityofCalifornia-Davis,USA.Alfalfaisonethemostwidelycultivatedforagelegumesworldwideandimproving alfalfa forage yield and nutritive value are major globalbreedinggoals.Genotyping-by-sequencing(GBS)providescosteffectivegenotypingthatenablehighresolutiongenome-wideassociationstudies(GWAS).Wepreviously used a panel of 365diploid individuals and 89SSR markers to map 23 forage yield and nutritive value-related traitsover two years. The limited marker number, unsurprisingly did notidentify any associations. Using GBS, we were able to conduct a trueGWAS. More than 15,000 genome-wide SNPs passed the qualitycriterionafterfilteringandwereusedforsubsequentanalyses.Wehavedetected a number of associations for all the traits evaluated and thetop associationswere located on theMedicago truncatula genome. ASNP in a coding region of a cell wall biogenesis gene associated withseveral cellwall related traits andwe suggest that itmay be the truecausative polymorphism. Two other SNPs residing in meristematicdevelopmentandearlygrowthgeneswerefoundtoassociatewiththetotal biomass yield. None of the SNPs associated with regrowth afterharvestorwithspringregrowthweremappedtoM.truncatulagenome.M. truncatula is an annual species related to alfalfa that typically haslimitedability toregrow.Thealleleswe identifywiththemajor impactonforageyieldandnutritivevaluecanberapidlyincorporatedintoourbreedingprogram.

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P70–S8 Unravellingseeddevelopmentinthecommonbean(Phaseolusvulgaris)integratingtranscriptomicsandproteomicsdataParreiraJ.R.1,BouraadaJ.2,Fitzpatrick,M.A.2,SilvestreS.3,4,BernardesdaSilvaA.B.3,4,MarquesdaSilvaJ.3,4,AlmeidaA.M.1,5,AltelaarA.F.M.2,Fevereiro,P.1,4,Araújo,S.S.1,61-PlantCellBiotechnologyLaboratory,ITQB-NOVA,2 -BiomolecularMassSpectrometryandProteomics,BijvoetCentre forBiomolecularResearch, Utrecht Institute for Pharmaceutical Sciences, University of Utrecht,NetherlandsProteomicsCentre,Padualaan8,3584CHUtrecht,TheNetherlands.3 - Plant Functional Genomics Group, Biosystems and Integrative Sciences Institute(BioISI),FaculdadedeCiências,UniversidadedeLisboa4 - Departamento de Biologia Vegetal, Faculdade de Ciências da Universidade deLisboa,CampoGrande,1749-016,Lisboa,Portugal.5-RossUniversitySchoolofVeterinaryMedicine,P.O.Box334,Basseterre,SaintKittsandNevis.6 - Plant Biotechnology Laboratory, Department of Biology and Biotechnology, ‘L.Spallanzani’,UniversityofPavia,viaFerrata1,27100Pavia,Italy.Common bean (Phaseolus vulgaris) is an important staple foodworldwide.Weconductedaproteomicandtranscriptomicanalysistounravel themolecularmechanisms ofP. vulgaris seed development(SD).Agel-freeproteomicassay(LC-MS/MS)wasdoneonseedsat10,20,30and40daysafteranthesis,spanningfromlateembryogenesisuntildesiccation.AttheearlySDstageanaccumulationofproteinsrelatedwith protein metabolism, glycolysis, TCA, stress and nucleic acidmetabolismwasobserved,reflectinganextensivemetabolicactivity.Atmid-SDtheaccumulationofstorage,signaling,starchsynthesisandcellwall-relatedproteinsstoodout.Inthelaterstages,anincreaseinproteins related to redox, and to a lesser extent proteindegradation/modification/folding and nucleic acid metabolismsreflect that seed desiccation-resistancemechanismswere activated.Cluesonmaintenanceofgenome integrityduringSDwereunveiled,suchasproteinsinvolvedinchromatinremodeling(histoneH2A)andDNArepair(proliferatingcellnuclearantigen).In parallel,we also investigated changes in gene expression profilesduring SD. For this purpose, we performed a Massive Analysis ofcDNAEnds(MACE)atthesametimepoints.The integration of information from both analyses enhances ourknowledgeontheSDmolecularmechanisms.Thisknowledgemaybeusedinthedesignandselectionofcommonbeanseedswithdesiredqualitytraits.

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P71–S8 Ahigh-densitygeneticlinkagemapandQTLanalysisofascochytablightresistanceinchickpeaDeokarA.A.,SagiM.,Tar’anB.UniversityofSaskatchewan,Saskatoon,Canada,amit.deokar@usask.caAscochyta blight causes significant yield loss in chickpea (Cicerarietinum L.) crop worldwide. To determine the genetic basis ofresistance to ascochyta blight in chickpea, we constructed a high-density genetic linkage map using genotyping-by-sequencing (GBS)and Illumina GoldenGate SNP genotyping approach on a chickpearecombinant inbred line population (CPR02) derived from a crossbetweentheAscochytablightpartialresistantchickpeacultivar‘Amit’andahighlysusceptiblecultivar‘ICCV96029’.Genotypingdataof664segregating SNPs were generated using 1,536 GoldenGate assays.Single enzyme (ApekI) based GBS and paired-end sequencinggenerated over 224.4 million reads with an average of 1.7 millionreads per line, which facilitated identification of 2,828 high qualitysegregating SNPs. Combining SNP genotyping data from bothplatforms, CPR02 linkage map consists of 3,460 SNP markers,distributed on eight linkage groups, covering a genetic distance of867.9 cM. The CPR02 population was evaluated in greenhouse andopenfieldconditionsandinvestigatedthequantitativetraitloci(QTL)associatedwithascochytablightdiseaseresistance.AtotalofsixQTLsforresistancetoascochytablight,locatedonchromosome2,3,4and5were identified. The identifiedQTLs explained 26.0% (greenhousestudy),39.9%(fieldstudy2014)and55.8%(fieldstudy2015)ofthetotal estimated phenotypic variation. Candidate genes physicallylocated in the genomic region associated with QTL were identifiedand genetically mapped to validate their colocalization with theidentified QTLs. This study provides useful genetic and genomicresources for marker-assisted breeding and better understandinggeneticsofascochytablightresistanceinchickpea.

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P72–S8 Genesinvolvedinfloweringtimeregulationinwhitelupin(LupinusalbusL.):transcriptomeprofiling,linkagemappingandphylogeneticinferenceKsiążkiewiczM.1,RychelS.1,TomaszewskaM.1,Nelson,M.2,NaganowskaB.1,WolkoB.11-InstituteofPlantGeneticsofthePolishAcademyofSciences,Poznan,Poland.2-RoyalBotanicGardens,Kew,MillenniumSeedBank,WakehurstPlace,Ardingly,UnitedKingdomWhitelupinsarevaluablegraincropswithhighproteinandoilcontent,lowalkaloidandlowfibre.Nevertheless,theircultivationinvolvesalongvegetativeperiod,whichmaybereducedinearlyfloweringaccessions.However, this trait appears to be under polygenic, recessive control,difficulttomanageinplantbreeding.Insight into European Lupin Collection led to identification ofvernalization independent,early floweringbreeding lines,mutantsandcultivars. Late lines,which flowered about 5-6weeks later,were fromwild populations. Mining of the Lupinus albus transcriptome forflowering regulation genes tagged 34 of 53 Arabidopsis thalianahomologs. Protein-based hiddenMarkovmodel annotation resulted inpredictionoffull-lengthproteinsfor27genes.Thenumberofpredictedcopies varied from 1 (AGAMOUS-LIKE 19, EARLY FLOWERING 1 and 3,FRIGIDA,FLOWERINGLOCUSY,VERNALIZATIONINDEPENDENCE3)to8-10 (EARLY FLOWERING 6, EARLY IN SHORT DAYS, VERNALIZATION 1).MassiveanalysisofcDNAendsof20linesdifferinginfloweringtimewasperformedtosurveyfloweringregulationpathwayandtoupgradewhitelupin gene index. Representative homologs were localized in thereference linkage map. Phylogenetic inference encompassing legumefamily revealed complex pattern of independent, lineage-specificduplications.Financial support:Ministry of Agriculture and Rural Development Task39/2016.Seeds:WesternAustralianAgricultureAuthority,PoznanPlantBreedersLtd."

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P73–S8 Physicochemicalandcookingpropertiesofdrybean(PhaseolusvulgarisL.)andsoybean(GlycinemaxL.Merr.)seedsBorosL.,WawerA.PlantBreeding&AcclimatizationInstitute-NRI,05-870Radzików,Poland.Physicochemical and cooking properties were determined in 23 drybean local populations together with 2 cultivars from the PolishGenebank and in 9 soybean genotypes adapted to northern latitude,which included seven cultivars and two breeding lines.Wide variationamong local populations of dry bean were observed in seed weight,hydrationcapacity,swellingcapacityandelectricconductivitywithCV%values 30, 32, 33 and 35 respectively, suggesting that there areconsiderable levels of genetic diversity. Substantial variations werefoundinseedcoatcontent(CV%14)andcookingtime(CV%17)amongdry bean populations. Tested soybean genotypes differed significantlyfor all evaluated traits, however with much lower variation amonggenotypes,exceptforcookingtime,ascomparedtodrybean,indicatingnarrowgeneticdiversity.Few significant correlation coefficientswere common for both speciese.g. seed coat content was negatively correlated with hydration andswelling capacities and positively with conductivity of soaking water.Cooking time was negatively correlated with conductivity of soakingwater and with hydration index. The results showed substantialvariability in dry bean and soybean in cooking time. From breedingstandpoint,highvariability suggest that it shouldbepossible toobtainappreciable responses in selection for this trait and develop cultivarswithdecreaseincookingtime.

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P74–S8 TheLathyrussativusgenomeproject.SarkarA.,EdwardsA.,EmmrichP.M.F.,MartinC.,WangT.L.TheJohnInnesCentre,Norwich,NR47UH,UnitedKingdom.Grasspea(Lathyrussativus)isahardy,droughttolerantlegumegrownforanimal feed, foodand fodderonpoorsoilswithminimal inputs. Itserves as an insurance crop for marginal farmers in the Indiansubcontinent and Africa. It is a diploid (2n=14) with an estimatedgenomesizeof6.9Gbp(BennettandLeitch,2005.AnnalsofBotany95:45-90).Itsmaindrawbackisthepresenceofaneurotoxin(beta-ODAP),which can cause neurolathyrism in people subsisting on apredominantlygrasspeadietforanextendedlengthoftime.WearecurrentlysequencingthegrasspeagenomeofaEuropeanline(LS007). A TILLINGpopulation is also being created for this line. A denovo shotgun sequencing strategy has been adopted based on theconstruction of a PCR-free library (using the Discovar protocol) andmate-pair libraries for Illumina sequencing. The draft genomewill beannotated using ours and others’ transcriptome data and data fromcompletedgenomesequencesofrelatedlegumes.The genome sequence will be valuable for the identification of thegenes in the beta-ODAPbiosynthesis pathway, aswell as for traits ofagronomic importance. The data will also provide insights intocomparativegenomicsacrosslegumes,helpinthedevelopmentofhighquality genetic and physical maps for marker-assisted and genomicselection strategies and enable a genome-editing platform for grasspea.

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P75–S8 Beanadapt:thegenomicsofadaptationduringcropExpansioninbeanPapaR.1,JacksonS.A.2,GeptsP.3,GranerA.4,FernieA.R.51-MarchePolytechnicUniversity,Ancona,Italy2-UniversityofGeorgia,Athens,GA3-UniversityofCalifornia,Davis,CA4 - Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben,Germany5-MaxPlanckInstituteofMolecularPlantPhysiology,Potsdam-Golm,GermanyBEAN_ADAPT is a three-year project funded through the 2nd ERA-CAPS call, ERA-NET for Coordinating Action in Plant Sciences. Themain aim of this project is to dissect out the genetic basis andphenotypicconsequencesoftheadaptationtonewenvironmentsofthe common bean (Phaseolus vulgaris L.) and its sister species, therunner bean (Phaseolus coccineus L.), through the study of theirintroduction, from their respective centers of domestication in theAmericas,andexpansionthroughEurope,asarecentandhistoricallywell-defined event of rapid adaptation. A large collection (11,500accessions of both species) from three major genebanks, will becharacterised by genotyping-by-sequencing (GBS), to define thepopulation structure and to obtain subsets of genotypes forphenotyping (fieldandgrowthchamber)and foradeepergenomic–transcriptomic–metabolomic characterisation. We will use amultidisciplinary approach: genomics (WGS and RNAseq),population/quantitative genetics, biochemistry, plant physiology onthesubsetofsamples.Differentialexpressionanalysis,analysisoftheco-expressionpatterns,andGWASwillbeusedtoidentifygenesandmetabolites putatively associated with adaptation, while genotypicinformationobtainedfromRNA-seqdatawillbeused,withGBSandWGS data, to test for signatures of selection. Among the mainoutcomes of the project are the development in P. vulgaris ofhaplotypesofall10,000accessions(HapBean).

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P76–S8 Mendel'senduringlegacy:orthologsoftwoofhissevenfactorsinmultiplecurrentdaycroplegumesPenmetsaR.V.1,Carrasquilla-GarciaN.1,StonehouseR.2,MirR.R.3,SaniG.1,RezaeiM.2,ChangP.1,CordeiroM.4,Ta’ranB.2,vonWettbergE.5,CookD.1,BettK.21-UniversityofCalifornia,Davis,USA2-UniversityofSaskatchewan,Saskatoon,SK,CANADA3-Sher-e-KashmirUniversityofAgriculturalSciences&Technology,Jammu,INDIA4-UniversityofSouthernCalifornia,LosAngeles,USA;5FloridaInternationalUniversity,Miami,USAWecharacterizedmorphologicalvariantsforseedcolorinchickpeaandlentil.Analysisofsequencevariationincandidategenesfrombiologicalpathways suggested by the morphological phenotypes, cross-legumesynteny,mining of draft whole genome sequence data, and sequencecharacterization of germplasm suggest thatmorphological variation inchickpeaandlentilarelikelyfromorthologsofMendel'sAandIlociforpurple versus white flower color and yellow versus green cotyledons.The identification of the molecular basis for the chickpea and lentilmorphological variation has practical implications for cropimprovement. The growing evidence for conserved function acrosslegumes suggests a facile route to the identification of the molecularbasisofmorphologicalvariationinotherlegumeswithlimitedgenomicsandsequencingresources.

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P77–S8 5-azacytidineandhydroxyureaaffectgerminationandseedlingdevelopmentPaivaJ.A.1,PaganoA.2,GualtieriC.1,2,GomesC.1,FevereiroP.3,AraújoS.S.2,3,BalestrazziA.21 - Department of Integrative Plant Biology, INSTYTUT GENETYKI ROŚLIN POLSKIEJAKADEMIINAUK,ul.Strzeszyńska34,60-479Poznan,Poland2 -Department of Biology andBiotechnology 'L. Spallanzani', via Ferrata 1, 27100-PaviaItaly3-PlantCellBiotechnologyLab,ITQBNOVAAv.daRepública,2780-157OeirasPortugalEpigeneticmarks,metastable changes ingeneexpressionand functionthatdonotresultfromchangesinDNAsequence,aremediatedbyDNAmethylation, histone modifications, and small interference RNAs.Cytosin-5 methylation (Cy5Met) is a major and dynamic epigeneticmodification, which is established and maintained by multiple,interactingcellularmachineries.Cy5Metofpromoterregionsandgenebodiesisveryimportantingeneexpressionregulation(bycisandtransacting).WeareinterestedintoinvestigatehowthemodificationofDNAmethylation patterns impact on plant development, namely on seeddevelopmentandgerminationandonsecondarycellwallsmodification,in the legume model plant Medicago truncatula Gaert. In thispreliminary essay we test the effect of different concentrations of 5-azacytidine (100,50,25,0uM;demethylationagent)andhydroxyurea(50, 10, 1 and 1mM; hyper-methylation agent) on germination ofM.truncatula.Weobservedasignificantreductionofthegrowthbetweenthehigherconcentrationandwaterforbothtreatment,andfor50mMhydroxyureagerminationoccurbutnofurtherdevelopmentandgrowthof the seedlings were observed. These phenotypic results will bepresentedanddiscussed together theglobal levelsofmethylationandgeneexpressionofsomegenesrelatedwithchromatinremodeling.

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P78–S8 GeneticvariabilitystudyandkaspmarkermappinganalysisrevealedanewhaplotypedeterminingpalehilumHcinViciafabaKhamassiK.1,JeddiF.B.2,O’SullivanD.M.31-UniversityofCarthage,FieldCropLaboratory,NationalAgriculturalResearchInstituteofTunisia,RueHédiKarray2049Ariana,Tunisia2-UniversityofCarthage,NationalInstituteofAgronomyofTunisia.43av.CharlesNicole,1082CitéMahrajène–Tunis3-SchoolofAgriculture,PolicyandDevelopment,UniversityofReading,Whiteknights,UKPhenotyping and genotyping of the Tunisian faba bean populations vstwo local commercial varieties (Badï and Bachaar) andworld diversitycollection,revealedsignificantdiversity.Consequently,threeindividualscarrying Pale Hilum (PH) were identified within two Tunisianpopulations.SeveralstudiesmentionedthatPHtraitwasinlinkagewithlow vicine-convicine (VC) gene (VC-) that reduces 20 fold these twoantinutritionalfactors.Thus,VCwerequantifiedinthethreeTunisianPHindividuals as putative candidate for VC-gene, Badï and Bachaar andother PH-varieties from different origins and compared to the checkvarieties Disco andMelodie carrying PH and VC-. Results showed thatthe PH trait in the Tunisian individuals is not associatedwith zeroVC.However,someofthemweredistinguishedbytheirsignificantlowerVClevelscomparedtotheTunisianandinternationalcommercialcultivars.A separate genetic mapping study using a segregating biparentalpopulation developed previously at NIAB (UK) was carried out. Thisanalysis allowed the identification of new kasp marker linked to palehilum traits segregating in the population. Genotyping of the F2generation and plant and seed phenotyping over F2:3 and F3:4generations were conducted. The Mendelian factors Hc, controllingHilumcolour,werelocatedinlinkagegroups2.ThemappedmarkerforHc allowed the identification of a new haplotype within the TunisianindividualsthatdifferfromtheEuropeanPHcultivars.

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P79–S8 Identificationofthetranslocationbreakpointbetweenchromosome4and8inthegenomesofMedicago

truncatulaA17andA20SzabóZ.1,BaloghM.1,MiróK1,DebelleF.3,CookD.R.4,EllisT.H.N.,KissG.B.1,2,KalóP.1 1-NARIC,AgriculturalBiotechnologyInstitute,Gödöll_,Hungary 2-AMBISBiotechnologyandResearchLtd,Szeged,Hungary 3-INRA,Toulouse,France 4-UniversityofCaliforniaDavis,Davis,California,USAExtensive genomic resources have been developed for Medicagotruncatula in the last two decades that made this species one of themodel legumes. A previous genetic analysis of the F2 mappingpopulationderivedfromthecrossofMedicagotruncatulaA17andA20detectedanunusual linkagephenomenonbetweenmarkers located inthelowerarmsofchromosome4and8(Choietal.2004).Itwasfoundthat some markers displaying high degree of genetic linkage showedcruciform-like linkage and no linear geneticmap could be constructedbased on the detected recombination events between thesemarkers.Themappositionofthesemarkerswereresolvedbypositioningthemtoeitherlinkagegroup4or8usingthemappingpopulationofM.sativa.AsubsequentstudyindicatedthataccessionA17ofM.truncatulacarriesatranslocation between chromosome 4 and 8 that resulted in reducedviabilityofF1pollens(Kamphuisetal.,2007)whencrossingwithotherM.truncatulaaccessions.In order to pinpoint the breakpoint of the translocated chromosomalsegmentsinA17,astudywasinitiatedusingfurthergeneticmappingofanother segregating population of M. truncatula combined with thesystematic sequencing of BAC clones using recently developed geneticmarkersaroundthebreakpointofthechromosomalrearrangement.Weare going to present how the identification of the translocationbreakpointhasbeenprogressed.

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P80–S8LiftingthecurseofthegrasspeaEmmrichP.M.F.,SarkarA.,EdwardsA.,MartinC.,WangT.L.TheJohnInnesCentre,Norwich,NR47UH,UnitedKingdom.Grass pea (Lathyrus sativus) is a legume crop with exceptional yieldstability in the faceof drought, flooding andpoor soils. This hardinessgivesthecropgreatpotentialforfoodsecurity,especiallyinthefaceofclimate change. However, this ancient food and feed crop has notreceived sufficient attention frombreeders due to its associationwiththeneurological disease Lathyrism,which is causedby the toxin beta-ODAP,whichisproducedbytheplant.We are working to remove this crucial limitation on the utilisation ofgrasspeabydevelopinglow/zero-toxingenotypes.Tothisend,wehavescreened natural germplasm and an EMS-mutagenized population,identifyingnewgenotypeswithlowertoxinlevelsthanexistingvarieties.In addition, we have sequenced transcriptomes derived from seventissues of grass pea. By interrogating this dataset, we have identifiedcandidate genes for some of the metabolic enzymes involved in thebeta-ODAPsynthesispathway,whichwearenowtestingbiochemically.Thismay allow us to elucidate the genetic basis of the production ofbeta-ODAP and pave the way for the first ever toxin-free grass peavarieties.

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P81–S8 MicroRNAexpressionduringinductionofM.truncatulasomaticembryogenesisCostaS.F.1,CostaO.C.1,FevereiroP.1,2,DuqueA.S.11 - Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova deLisboa,Av.daRepública,2780-157Oeiras,Portugal2 - Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa,CampoGrande,1749-016Lisboa,PortugalPlant somatic embryogenesis (SE) is a highly complex developmentprocess essential for genetic engineering, plant improvement andclonalpropagation.Ahighlyembryogenic lineofM.truncatula cv Jemalong (M9-10a)wasderived from a non-embryogenic line (M9), as a result of somaclonalvariation.Thesetwolinesareidealtoolsforstudyingembryogenesisasthey share an extremely similar genetic background, differingessentiallyatthelevelofSEability.MiRNAs play crucial roles in plant SE since they modulate geneexpression to enable pattern formation during plant embryogenesis.QuantitativePCRisastandardmethodologyforquantifyingmiRNAduetoitsspecificityandreproducibility.Howeveritsaccuracyreliesontheuseofonproperdatanormalization.Herewe compare the expressionprofiles of five siRNAs (Mt si4, si41,si65, si106 and si190) with the commonly used U6 snRNA during SEinduction process in M9/M9-10a lines to find candidate referencesRNAs, using NormFinder and geNorm algorithms.We identified si65andsi106asthebestnormalizers.Selected si65/si106 were subsequently used for data normalizationwith Pfaffl analysis of five conserved miRNAs (mir159a, mir166a,mir166g, mir167a and mir172a) during 21 days of SE induction. Ourresults show significantly differences in miRNAs expression profilesbetweenM9/M9-10alinesovertime.Moreover,selectedsiRNAscouldbe considered more appropriate than the U6 snRNA for qPCR datanormalization

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P82–S8 NovelinvivoprotocolforevaluationofconstitutiveandseedspecificpromoteractivityinimmaturelegumeseedsŠvábováL.1,vanHaesendonckG.2,NavrátilO.2,PlchováH.2,JindřichováB.2,PloužkováT.2,3,ČeřovskáN.2,GrigaM.1,MoravecT.21-PlantBiotechnologyDepartment,AgritecPlantResearchLtd.,Šumperk,CzechRepublic2-LaboratoryofVirology,InstituteofExperimentalBotanyCAS,Prague,CzechRepublic3-DepartmentofBiochemistry,CharlesUniversity,Prague,CzechRepublicLeguminous seeds are attractive expression systems for production ofrecombinant proteins such as edible vaccines or antibodies.Nevertheless,efficientand stablegenetic transformationof legumes isstillnotaroutineandthewholeprocesstoobtainT0transformantsmaytake 9 to 12 months. In addition the reasonable production ofrecombinant proteins of interest must be directed to the seeds, byorgan-specific promoters. Novel in vivo protocol was developed toassess various promoter activity of different constructs containing gusreportergene incombinationwithpromoters35S,nosP,gly,βconandole for soybean and pea. In the preliminary series of experiments westudiedtheeffectofco-cultivationtreatments(sonication)andchemicaladditives (acetosyringone, dithiothreitol, L-cysteine). The protocol wascarriedoutinnon-sterileconditionsonplantsgrownintheglasshouse.Agrobacterium suspension was applied to scored seeds in pods andwrappedwithparafilm for6days.Quantitativegusassaywasdoneonharvestedseedstoevaluatetransientgeneactivityofconstructs.Usingnovel invivoprotocolwewereable toachievesufficientexpressionofrecombinantDNAin immatureseedsandtoperformtheirquantitativecomparison. The protocol could be improved using changes inAgrobacterium strain, sonication time/intensity, presence/absence ofchemicaladditives.Acknowledgement:ThisresearchwasfinanciallysupportedbyCSF,grantNo.15-10768S.

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P83–S8 FinemappingofAscochytablightresistanceinchickpeaVermaP.NationalInstituteofPlantGenomeResearch,NewDelhi,IndiaAscochyta blight (AB) is a devastating disease that affects chickpea(Cicer arietinum), a major legume crop. Conferring resistance inchickpea to AB is a prominent aim of chickpea breeders. Studiesconducted in thepast using classicalmarkers havepointedout thatresistance to AB is partial or incomplete with its inheritance beingquantitative.WeadoptedNGSresequencing-basedmultipleQTL-seqapproach to identify QTLs for AB resistance in chickpea usingpopulationbulksderivedfromtwocrosses,oneinter-specificandtheotherintra-specific.WefocusedontwomajorQTLsandproceededtoconfirm the NGS data with classical QTL analysis using thirteenpolymorphicmarkers.We could bring down the area of one of themajorQTLstoanarrowregionof28Kb,whichharbouredfourgenes.Expression analysis of these four genes under AB stress showedinduced expression of two genes in the resistant variety, bothbelonging to the same family and they interact physically in-planta.Interestingly,thisgenefamilywasalsoidentifiedinotherminorQTLsresponsibleforAB.Thesegeneswerefurtheremployedforfunctionalcharacterization.TheareaunderthisQTLwassequencedindifferentaccessionsof chickpea that are either resistant or susceptible toABfor association of the trait to the gene(s).Markers developed by uscanbeusedinbreedingprogrammesandotherpopulationstudies.

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P84–S8MutationsintheMFNgenesleadtoanincreasedproductionofpodsandseedsinpeaMartínez-FernándezI.1,FourquinC.1,BerbelA.1,DalmaisM.2,SmykalP.3,AnnichiaricoP.4,BendahmaneA.2,MadueñoF.1,FerrandizC.11-InstitutodeBiologíaMolecularyCelulardePlantas,CSIC-UPV,Valencia,Spain2-UnitédeRechercheenGénomiqueVégétale,UMRINRA-CNRS,EvryCedex,France3 -Department of Botany, Faculty of Sciences, Palack_University inOlomoucOlomouc,CzechRepublic.4-ResearchCentreforFodderCropsandDairyProductions,ARC,Lodi,ItalyAnnual legume plants, such as pea, go through a vegetative phase inwhich it the shoot apex produces nodes with lateral vegetativebranches. After floral transition the shoot apex becomes a primaryinflorescence that, while it is active, produces flowering nodes, withlateral secondary inflorescences with flowers. The production of podsand seeds depends on the number of flowering nodes formed by theplant.We have identified two genes, which we named MORE FLOWERINGNODES (MFN), encoding homologous transcription factors that controltheperiodoftimeinwhichtheprimaryinflorescenceapexisactiveand,therefore,thenumberoffloweringnodes.Homozygousplantswithnullmutations in MFNa or MFNb, grown under optimal greenhouseconditions, produce up to twice the number of pods and seeds thantheircorrespondingwild-typeparental line,withoutaffectingfloweringtime.To understand whether the MFN genes can be used to increase theproductionofseedsinpea,weareanalysingindetailthephenotypeofthe mfn mutants. In addition, to analyse their performance incommercial varieties, we are currently introgressing the mfn mutantalleles into several pea elite cultivars. Finally, we are also looking fornewmfn alleles, potentiallymore advantageous, in collections ofwildpeaaccessionsandcultivarstoestablishtheirpossiblecorrelationwiththenumberoffloweringnodes.WeexpectthatMFNgenescanbeusedasausefultooltoincreaseyieldingrainlegumes.

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Session 9, parallel: Legumes in food and feed andotheralternativeuses(cont.)-RoomArrábidaIIIChaired by Ruta Galoburda (Latvia Univ. of Agriculture,Latvia)andTomWarkentin(Univ.Saskatchewan,Canada)

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OralCommunications14:30-14:40 Oral–S9Ameta-analysistoassesstheeffectoffinegrinding,dehullingandpelletingonthenutritivevalueofpeasandfababeansforpoultryTormoE.1,HeuzéV.2,PeyronnetC.1,TranG.2,ChapoutotP.2,31-TerresUnivia,11ruedeMonceau,CS60003,75378ParisCedex08,France2-AFZ,16rueClaudeBernard,75231ParisCedex05,France3-UMRMoSAR,INRA,AgroParisTech,UniversitéParis-Saclay75005ParisFranceThenutritivevalueofpea (P)and fababean (FB) canbe improvedbymechanical and heat treatments, such as grinding, dehulling andpelleting. Datawere collected from18 publications and processed bymeta-analysis to assess these effects and obtain within-sample orwithin-trial prediction models. Fine grinding improved starchdigestibility(STd)andmetabolisableenergycontent(AMEn)ofPandFBin non-pelleted diets, with a greater effect for broilers than forroosters: a reduction of the median diameter of 100mm lead to anincreaseof 4.2vs 1.4points%of STd andof 169vs 39kcal/kgDMofAMEn for broilers vs roosters. Dehulling improved AMEn for coloredandwhite-floweredFB,butonly for tannin-richP.Proteindigestibility(CPd) was improved for tannin-rich varieties of both grains. Crudeproteincontent(CP,%DM)isthebestcriteriatopredictAMEnandCPd:AMEn=106.4CP-484 (FBdata,N=20;Nsample=10;R²=0.97;RSD=82)for FB; CPd=3.06CP-19.2 (N=16; Nsample=8; R²=0.95; RSD=2.0) forboth tannin-rich grains. Pelleting improved feed AMEn and Std,howeverthegainobtainedbypelleting(DAMEn,DSTd,%initialvalues)was negatively correlated with initial grain values (AMEn0, Std0):DAMEn=117-0.037AMEn0(N=30;Ntrial=13;R²=0.95;RSD=2.1) forPandDAMEn=50-0.015AMEn0(N=12;Ntrial=2;R²=0.69;RSD=3.1) forFB.Dueto lackofdataonSTd,onlyonemodelwasobtainedforbothgrains:DStd=83.6-0.894Std0(N=23;Ntrial=10;R²=0.97;RSD=1.5).

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14:40-14:50 ORAL–S9ProteinqualityofdifferentPortuguesevarietiesofcommonbean(PhaseolusvulgarisL.):themissingdataforbreedingMechaE.1,FigueiraM.E.2,VazPattoM.C.1,BronzeM.R.1,2,31 - Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. DaRepública,EAN,2780-157Oeiras,Portugal.2 - Faculdade de Farmácia, Universidade de Lisboa, Av. das Forças Armadas, 1649-019Lisboa,Portugal.3 - Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras,Portugal.emecha@itqb.un.ptCommonbean(PhaseolusvulgarisL.) isconsumedworldwideasarichsource of inexpensive vegetable protein, particularly relevant forpreventing protein deficiency status in developing countries (whereanimalproteinisnotalwaysavailable).Characterized by their high content in aminoacids such as lysine andlow content in sulphur containing aminoacids, common beanscomplementedbycerealsprovidealltheessentialaminoacidsandcanbeusedasanalternativetoanimalproteininhumandiet.Geneticsandenvironmenthavebeenpointedasdeterminantfactorsinprotein quality of common beans, affecting aminoacid composition.Despite the interest of breeders in improving protein quality ofcommon beans, the diverse Portuguese traditional varieties remainunderstudied. Characterizationof such varietieswill contribute to thedevelopment of interesting germplasm and tools for future breedingapproaches. In order to analyse protein quality of Portuguesegermplasm, 108 different varieties were multiplied in a randomizedcontrolled field trial andaminoacids’ contentdetermined in triplicate,after chemical hydrolysis, using High Performance LiquidChromatography (HPLC) coupled to Fluorescence (FL) detection. Toestimate protein digestibility, a fast screening method based onenzymatichydrolysisandpHvariationwasapplied.Finaldata, treatedbymultivariateanalysis,allowedsystematizationofdataandbridgethegapofknowledgeinproteinqualityofcommonbeans.

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14:50-15:00 Oral–S9EconomicfactorsofusingthelegumesinbroilerchickensfeedingProskinaL.,SallijaC.,KonosonokaH.ILatviaUniversityofAgricultureTheproductivity,meatqualityandproductioncostofbroilerchickensaredirectlyassociatedwithcrudeprotein inadietfedtothebroilerchickens as well as its content, biological value and price. Soybeanprotein,whichisarelativelyexpensiveimportedfeedstuff,makesup,onaverage,25%ofanypoultrydiet. In theEUMemberStates, feedprotein imports comprise 70% of the total feed imports, whichcontributes to production cost increases. An analysis of domesticprotein-rich feedstuffs shows that faba beans and peas contain,respectively,200-230gkg-1and260-380gkg-1crudeprotein,whichallows replacing imported protein rich feed with domesticallyproduced protein-rich feedstuffs, thereby reducing feed productioncost and the proportion of imported feed in livestock diets. Theresearchaimisto identifyeconomicgainsfromfeedingdomesticallyproducedfababeansandpeastobroilerchickens.Theresearchfoundthatreplacingimportedsoybeanswithfababeansandpeasinbroilerchickendietsreducedfeedcostsby0.06-0.16EURkg-1,increasedtheproduction efficiency factor (PEF) by 9.85-54.94 units as well asdecreasedthefeedconversionratioortheamountoffeedconsumedper kg live weight gain by 0.61-6.06% for the experimental groups,comparedwiththecontrolgroup.

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15:00-15:10 Oral-S9TocopherolsandcarotenoidsdiversityinachickpeagermplasmcollectionexploitedinchickpeabreedinginEuropeSerranoC.1,CarbasB.1,VazPattoM.C.2,BritesC.11 - INIAV, Instituto Nacional de Investigação Agrária e Veterinária, 2784-505 Oeiras,Portugal2-ITQBNOVA,InstitutodeTecnologiaQuímicaeBiológica,2780-157Oeiras,PortugalChickpea (CicerarietinumL.) is considered the5thvaluable legume interms of worldwide economic importance and a cheap source ofprotein,which can be largely explored by the processing industry forenhancingfoodnutritionalvalue.Thisgrain legume isalsoagoodsourceof importantvitamins suchasthevitaminAprecursor,β-carotene,andVitaminEliketocopherols(α-T,β-T,γ-T,δ-T)andtheirtocotrienolcounterparts(α-T3,β-T3,γ-T3,δ-T3).Theobjectiveofthisresearchwastoexaminethevariationofbioactivecompoundsliketocopherolsandcarotenoidsin86chickpeaaccessionsrepresentingthechickpeagermplasmdiversity inusebytheEuropeanbreeders. These analyses were performed under the FP7-EU LEGATOproject.Thetocopherolsandcarotenoidsinchickpeaflourswerequantifiedbynormal phase high performance liquid chromatography. Thetocopherolswereexpressedinthefatfractionandmeanconcentrationforγ-tocopherolwas626.59μgg-1,followedbyα-tocopherol139.11μgg-1 and δ-tocopherol 31.96 μg g-1. The highest concentration of thedifferent tocopherols determined in the fat fraction was found inLEGCA717 for γ-tocopherol (1078.76 μg g-1) followed by LEGCA602(216.75 μg g-1) for α-tocopherol, while the highest contend of δ-tocopherolwasfoundinLEGCA674fatfraction(57.35μgg-1).The carotenoids were expressed in whole flours and the mean forluteinwas5.43μgg-1folowedbyzeaxantin4.38μgg-1.

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15:10-15:20 Oral–S9Bowman-BirkinhibitorsfromlegumesandmammalianguthealthClementeA.1,Marín-ManzanoM.C.1,ArquesM.C.1,OlíasR.1,RubioL.A.1,DomoneyC.21-EstaciónExperimentaldelZaidín(CSIC),Granada,Spain.alfonso.clemente@eez.csic.es2-JohnInnesCentre,Norwich,UnitedKingdom.Bowman-Birkinhibitors(BBI)fromlegumes,suchassoybean,pea,lentilandchickpeaareresistanttomammaliangutproteolysis;theirintrinsicability to inhibit serine proteases is not significantly affected by themetabolic/enzymatic activity of gut microbiota. In vitro and in vivostudies have proven that soybean BBI and homologous proteins canexertaprotectiveand/or suppressiveeffectagainst inflammationandcancerdevelopmentwithinthegastrointestinaltract.Wehave investigatedtheeffectsofapeaalbuminextractenriched inBBI (PPIC) in a mouse colitis model. Macroscopic and histologicalstudies, together with analysis of colonic expression of pro-inflammatory markers, showed that a severe inflammatory conditionwasamelioratedinextentandseveritybyPPIC.We have described a significant concentration- and time-dependentdecreaseintheproliferationofhumancolorectaladenocarcinomacells,following treatmentwithBBI variants frompea, lentil and soybean.Amajor pea protease inhibitor, TI1, expressed in Pichia pastoris, andrelatedengineeredmutantshavingmodifiedinhibitoryactivitysuggestthatBBIproteinsaretakenupbycoloncancercellsandexerttheiranti-proliferative properties via protease inhibition. Serine proteases thatbecome active in early stage of colorectal carcinogenesis are likely torepresentaprimarytargetofBBI.

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15:20-15:30 Oral–S9EvaluationofapeagenomewideassociationstudypanelforfolateprofilesbyUPLC-MS/MSJhaA.B.,PurvesR.W.,GaliK.,Tar’an,B.,VandenbergA.,WarkentinT.D.CropDevelopmentCentre,UniversityofSaskatchewan,51CampusDrive,Saskatoon,SKS7N5A8,Canada.ambuj.jha@usask.caFolatesareimportantcofactorsinseveralmetabolicfunctionsincludingthebiosynthesisofnucleicacids.Pulsecropseedsarerichinfolates.Inour previous studies, six different folates were quantified by ultra-performance liquid chromatography coupled with mass spectrometry(UPLC-MS/MS) in seedsof fourcultivarseachofcommonbean, lentil,chickpea and pea developed at the Crop Development Centre (CDC),University of Saskatchewan. Compared to the other crops, pea had arelatively low concentration of total folates. A wider survey of peaaccessions may reveal useful variation for improvement of folateconcentrationorprofile.Withthisobjective,research is inprogresstoevaluateapeagenomewideassociationstudypanelof177accessionsdevelopedattheCDCconsistingofcultivarsandlandracesfromNorthAmerica, western and eastern Europe, and Australia for folates usingUPLC-MS/MS. These accessions were genotyped by genotyping bysequencingandanIlluminaGoldenGatearray,andhavebeengrownatSaskatoon,NorthDakota,WashingtonState,CzechRepublicandSerbia.Usinggenome-wideassociationandcandidategeneapproaches,folateprofileswill be associatedwith genotyping data to identify significantSNPsformarker-assistedselection.

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Session10,parallel: Frontiers in legumegeneticsandgenomics(cont.)-RoomArrábidaIVChaired by Bernadette Julier (INRA, France) and KevinMcPhee(NorthDakotaStateUniv.USA)

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OralCommunications14:30-14:40 Oral–S10Aproteinquantitylociapproachcombinedwithagenome-wideassociationstudyrevealedregulatorsofproteinaccumulationinlegumeseedsLeSignorC.1,AimeD.1,SanchezM.1,YoungN.D.2,ProsperiJ-M.3,ThompsonR.D.1,BuitinkJ.4,BurstinJ.1,GallardoK.11-Agroécologie,AgroSupDijon,INRA,Univ.BourgogneFranche-Comté,F-21000Dijon,France2-DepartmentofPlantBiology,UniversityofMinnesota,St.Paul,Minnesota,55108,USA3 - UMR AGAP, Equipe Génomique évolutive et gestion des populations, InstitutnationaldeRechercheAgronomique(INRA),Montpellier,34060,France4-IRHS,INRA,AGROCAMPUS-Ouest,Universitéd’Angers,SFR4207QUASAV,749071Beaucouzécedex,FranceTheseedsofgrainlegumesprovideproteins(20-40%proteincontentdepending on species) for human nutrition and animal feed. Thenutritional and technological quality of legume seeds is mainlydeterminedbythecompositionandfunctionalpropertiesoftheseedprotein fractions. By accounting for up to 70% of total proteins inmature seeds, theglobulinsaremajordeterminantsof seedquality.Inpea,which isoneofthegrain legumesmostcultivated inEurope,thevariability intheabundanceofglobulinpolypeptidesacross linesissignificant,whichmightenableawiderangeoffoodapplication.Toprovideknowledgeabout themoleculardeterminantsunderlyingdifferences in seed protein composition, we have used the modellegume species M. truncatula for which extensive genetics andgenomics resources were available (Young and Bharti, 2012), alongwith comprehensive transcriptomics data related to seeddevelopment (Benedito et al., 2008; Thompson et al., 2009). A PQLapproachidentifiedthegenomicregionscontrollingvariationsinseedprotein composition, and a translational approach exploiting theresources developed forM. truncatula and pea provided a set ofcandidategenesunderlyingPQLsconservedbetweenthetwospecies.The candidate gene selection was then refined by genome-wideassociation studies (GWAS), which enabled the identification ofnucleotide variations associatedwith variations in globulin synthesisand/ormaturation.

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14:40-14:50Oral–S10UsinggenomicstodecipherthegrainlegumesqualityriddleVazPattoM.C.1,AlvesM.L.1,MechaL.1,BentodaSilvaA.1,LeitãoS.T.1

,AlmeidaN.1,GonçalvesL.1,DiegoRubiales2,BronzeMR1,3,41 - Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras,Portugal.2-InstituteforSustainableAgriculture,CSIC,Cordoba,Spain.3-iBET,InstitutodeBiologiaExperimentaleTecnológica,Portugal,4iMED,FaculdadedeFarmácia,UniversidadedeLisboa,Portugal.Today's consumers are increasingly concerned with food quality andsustainability.Grainlegumescouldplayanimportantroleinansweringthese concerns. Grass pea (Lathyrus sativus) is a promising source ofcalories for drought-prone areas while common bean (Phaseolusvulgaris) is the most important edible legume worldwide. Yet thenonalignment of breeding objectives and end-users preferences ishamperingtheiruseinEurope.Breedingforimprovedend-user´squalityisacomplextaskduetotraitinteraction.Particularmetabolitesactasantinutrientsaswellashealth-promotingagents,influencingbothtasteandconsumers’acceptability.Under the scope of LEGATO, BEGEQA and QUALATY projects, westudied the genetic basis of several of these dual action bioactivecompounds(asphenoliccompounds,phyticacidor-ODAP)asaprimarystep for devising innovative quality breeding approaches for commonbeanandgrasspea.To reach thesegoals,100grasspeaand150commonbeanaccessionswererepeatedlyfieldtrialed.Seedmetabolitescontentswereanalyzedusing different analytical approaches and DNA screened using NGS-based SNP genotyping. A genome-wide association study, joiningbioactivecompoundscontentphenotypeswithgenomicinformation,isongoingusingmixedlinearmodelstoaccountforpopulationstructureandfamiliarrelatedness.Functional markers will be identified conferring consumer-drivenqualitytraits,toredirectbreedingtowardsmoreattractivevarieties.

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14:50-15:00 Oral–S10Strategiesandadvancestoidentifycandidategenescontrollinglowvicine-convicineinfababean(ViciafabaL.);GutiérrezN.1,2,BakroF.2,AtienzaS.3,AvilaC.M.2,TorresA.M.21-ZAYINTEC,edificioPITA-UniversidaddeAlmería,04120,Almería,Spain;2-IFAPA,CentroAlamedadelObispo,ÁreaMejorayBiotecnología,Apdo.3092,14080,Córdoba,Spain3-InstitutodeAgriculturaSostenible-CSIC,AlamedadelObispos/n,14004,Córdoba,SpainVicineandconvicine(v-c),limittheuseoffababeanasfoodandfeed.Asingle gene vc-, responsible for a 10-20 fold reduction in v-c, 10 cMapartfromthewhitehilumhasbeendescribed(Ducetal.1989;2004).Khazaeietal.(2015),reportedaQTLforv-ccontentonchromosomeI(ChrI)andalthoughseveralclosermarkerswereidentified(Gutierrezetal.2006)nocandidategenesareso faravailable.The identificationofsuitable candidate genes is limited for the lack of knowledge of thepathwayforthev-cbiosynthesisandthe largefababeangenomesize(~13 Gbp). In an attempt to determine which enzymes ortranscriptional regulators could be encoded by the vc-gene, we areapplying a combination of genetic linkage and comparative genomicapproaches. To facilitate high-throughput genome profiling DarTSeq(Kilianetal.2012)hasbeenappliedinaRILpopulationfromthecrossVf6(highv-c)xvc-(lowv-c)generatingmorethan10.000markers.Forthe assignmentof the linkagegroup to specific chromosomes, 58ESTanchormarkerfromthereferenceconsensusmap(Satovicetal.2013)wereassayedand14ofthemcouldbemapped.Ontheotherhand,37SNPs,fromtheKASParassayplatform(Semagnetal.2014)belongingtoChr I, were genotyped and 9 of them, resulted polymorphic. ThemoderateconservationofthefababeanChr1withtheMedicagoChr2confined the target region between Medtr2g005900 andMedtr2g026550.Tofinemappingthev-cposition,primersfor61newcandidatesweredesignedusingboth theMedicago (29) and the fababean (32) transcriptome sequences (Ocaña et al. 2015) and 12 genescould be mapped. The forthcoming linkage analysis may providepotentialcandidategenesforthetargettrait.

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15:00-15:10 Oral–S10ThehomologuesofArabidopsisFLOWERINGLOCUSTandGIGANTEAgenesareinvolvedinthecontrolofphotoperiodresponseoffloweringincommonbeanGonzálezA.M.1,Yuste-LisbonaF.J.2,Fernández-LozanoA.2,BretonesS.2,SussmilchF.C.3,WellerJ.3,LozanoR.2,SantallaM.11-GrupodeBiologíadeAgrosistemas (www.bas-group.es).MisiónBiológicadeGalicia-CSIC.P.O.Box28.36080Pontevedra,Spain2 - Departamento de Biología y Geología (Genética), Centro de Investigación enBiotecnologíaAgroalimentaria(BITAL).UniversidaddeAlmeria.04120Almeria,Spain3-SchoolofPlantScience,UniversityofTasmania,Hobart,Tasmania7001,Australia.In the common bean short-day plant, seasonal flowering is a crucialaspectofmaximizingreproductivefitness.Expansiontohigherlatitudesor changes in day length has been accompanied by earlier floweringunder long-day and a reduction in photoperiod responsiveness thatenables plants to anticipate approaching seasonal variation in thesurrounding environment. In the model species Arabidopsis thaliana,floweringtimeisdeterminedbyday-length–dependentinductionoftheFLOWERING LOCUS T (FT) gene, which encodes a floral-inductivemobile signal, and GIGANTEA (GI) gene acting upstream of FT. Thecharacterization of common bean homologues of Arabidopsisphotoperiodicfloweringpathwaygenesisreportedwiththeendgoalofacceleratingcommonbeanbreedingbyunderstandingthegeneticbasisofadaptation.TheexpressionofcommonbeanGIandFThomologuesunder short-and long-dayconditionswasexaminedusingqRT–PCR incommonbean lines,whichexhibitdifferentday lengthresponses.ThePvFTa3 and PvFTb1 homologues showed similar expression patternsindependently of the analyzed genotype, whereas significantdifferenceswerefoundintheGIhomologuePvGI1gene.Itisproposedthat these differences could contribute to the different day lengthresponses. Together, our results indicate that key genes controllingphotoperiodicfloweringinArabidopsisareconservedincommonbean,andaroleforthesegenesinthephotoperiodiccontrolofbulbinitiationispredicted.Thisworkwas financially supported by theMinisterio de Economía yCompetitividad (AGL2014-51809-R), Investigación y TecnologíaAgrariayAlimentaria(RF2012-C00026-C02-01andRF2012-C00026-C02-02)andUE-FEDERProgram.

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15:10-15:20 Oral–S10WildpeaPisumfulvumandPisumelatiuschromosomesegmentsubstitutionlinesincultivatedP.sativumgeneticbackgroundLenkaS.1,GregoireA.2,SmykalP.11 – Dep.of Botany, Palack University in Olomouc, lechtitel 27, 783 71 Olomouc, CzechRepublic.2-INRA,Dijon,FrancePlant evolution under domestication has altered numerous traits,introducing domestication bottleneck resulting in high degree ofrelatedness,leadingtonarrowergeneticbaseofcultivatedgermplasm,pronetopestsanddiseases.Thestudyofgeneticdiversityshowedthatalthoughwidediversityiscapturedamongcultivatedpea,wildmaterialprovides yet broader diversity (Smýkal et al. 2013, 2015). Thechromosome segment substitution lines (CSSL) containing genomicsegmentsofwildpea(PisumfulvumWL2140orPisumelatiusL100) inthecultivatedpea(P.sativumsubsp.sativumcv.Ternoorcv.Cameor)geneticbackgroundweredeveloped.Theselineshavebeenmolecularlyanalyzedusingmicrosatelliteandgene-specificmarkersat2 to82cMspacingatearlygenerations(BC2-3F2-4).Therewere5to14segmentsper line, withmean of 9.6. Higher density genotyping of 50 selectedBC3F6P.fulvum/TernoCSSLlinesusingpea13.2kPeaSNP(Tayehetal.2015) and further 100 lines using DARTseq approach is in progress.Establishment of such permanent introgression library will allowphenotypiccharacterizationofunlimitednumberoftargettraits,which,coupled togetherwith higher densitymarkers,will providemeans forQTL and gene identification and subsequent incorporation in desiredgenotypes. Field testing of agronomical performance of 50 lines ofP.fulvum/TernoCSSLlinesisunderway.This work received funding from the European Community's SeventhFrameworkProgramme(FP).

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15:20-15:30 Oral–S10DelimitingthephysicalpositionsofanthracnoseresistanceclustersCo-2andCo-3usingGbSinasetofcommonbeannear-isogeniclinesCampaA.,MurubeE.andFerreiraJ.J.PlantGeneticGroup,SERIDA,Asturias,SpainGenotyping by sequencing (GbS) analysiswas used in a set of beannear-isogenic lines (NILs)and thecorrespondingparents, inorder todelimit the introgressed genomic regions and approach to genesconferringanthracnoseresistance.GbSanalysisprovided50504SNPswithknownphysicalposition,distributedalongthe11commonbeanchromosomes. Introgressed regions tagging SNPs were detected atthe beginning of chromosome 4 in six NILs derived from resistancesources A493 and A321, both carrying an anthracnose resistancelocus at the Co-3 cluster. Among the six NILs there is a commonintrogressed region of 1.32Mb between physical positions 0.4-1.30Mb. In silico exploration into this region in the reference genomeshowed31genesassociatedtodiseaseresponses (Rgenes) thatarecandidate genes to be controlling the resistance. Two neighboringregions including 2 or 4 blocks were delimited in chromosome 11when common regions were investigated in ten NILs derived fromresistancesourcesA252andSanilacBc6Are,bothwithananthracnoseresistance loci at the Co-2 cluster. In this case, the regionsintrogressed from both sources are quite different, showing only acommonregionof0.09Mb,between46.9-47.1Mb,thatincluded16annotatedR genes. Large scale genotyping suppliedbyGbS analysishas enabled to discardmore than 99.8% of the genes annotated inthe bean reference genome, showing that can be rapid way toapproachtogenescontrollingimportanttraitsusingNILs.

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Session 11, parallel: Frontiers in plant and cropphysiology-RoomArrábidaIIIChaired by Christophe Salon (INRA, France) and LuisAguirrezabal(CONICET,Argentina)16:30-17:00 Key lecture - Phil Mullineaux: Theidentificationofnovelgenescontrollingplant-environmentinteractions

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OralCommunications17:00-17:10 Oral–S11Drought-inducedtranscriptomechangesinsoybean(GlycineMax)crownnodulesVorsterJ.,CilliersM.,vanWykS.,vanHeerdenR.,KunertK.University of Pretoria, Department of Plant and Soil Sciences, Forestry andAgriculturalBiotechnologyInstituteBiological nitrogen fixation is an important attribute of legumes.However, root nodule formation and their life span are negativelyaffectedbydroughtstress.Thereisanincreasingneedforselectionofimproved drought tolerant soybean cultivars to address future foodsecurity. In this study we investigated the response of soybean rootnodules exposed to various levels of water deficit conditions on aphysiologicalandtranscriptomicbasis.Wefoundjustover100genesinthe nodule transcriptome that were up regulated over all droughttreatments.Themosthighlyup-regulatedgenesasaresultofdroughtstress were a D11-LEA group 2 protein (Glyma.05G112000) and aninositol 3-alpha-galatosyltransferanse (Glyma.19G227800). We areparticularlyinterestedintheroleofcysteineproteasesdroughtinducedsenescence.We identified eight C1 and three C13 cysteine proteasesinduced by drought stress.One protease (Glyma.05G055700), a beta-leguamin C13 cysteine protease was highly expressed under droughtstressconditionscomparedtonaturalsenescenceandcouldprovideapossible marker for drought induced nodule senescence. Overall theresults identified genes that are responsible for causing prematuresenescenceofsoybeanrootnodulesasaconsequenceofdrought.

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17:10-17:20Oral–S11Invivomonitoringofthedevelopmentoflegumerootsystem,noduleandpoddevelopmentMetznerR.,ChlubekA.,DautzenbergM.,BreuerE.,WindtC.,PflugfelderD.,JahnkeS.Institute for Bio- and Geosciences (IBG) 2: Plant Sciences, Forschungszentrum JülichGmbH,52425Jülich,Germany.Quantitative non-invasive measurement of structural and functionaldevelopmentofplantorgansallowsfordeepphenotypinganddynamicinvestigationofplantperformanceunderstress.Whilethiscanbedonestraightforward for leaves or stems other plant parts, such as seedsenclosedinpodsorrootsandnoduleshiddeninsoil,aremoredifficultto investigate. Their development, however, is critical for yield andperformanceunder stress anddirectobservations in conjunctionwithgenetic and metabolomics approaches may therefore hint on theunderlying mechanisms. Here, we apply a set of three non-invasivetechniques for studying such developmental processes. 1) Low-fieldnuclearmagneticresonancerelaxometrywithportabledevices(pNMR)isusedtomonitordrymatterandwatercontent inpodsoverperiodsof several weeks. 2) Magnetic Resonance Imaging (MRI) is taken tostudythestructuraldevelopmentofrootsandnodulesinsoilfilledpotsover several weeks. 3) Positron Emission Tomography (PET) with theshort-lived radiotracer 11C is used to analyze the partitioning ofphotoassimilates and its dynamics among roots and nodules. Theapplicationofallthreetechniquestopeaandbeanplantsgrowninsoilwill be presented.We also discuss their potential to provide a directview on the effects of genotype or rhizobial strain on plantperformanceunderstressandonbiologicalnitrogenfixation.

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17:20-17:30Oral–S11AcommonshootdevelopmentalframeworkforperenniallegumespecieswithcontrastingmorphogenesisFaverjonL.,LouarnG.INRA,URP3F,Lusignan,FranceAwiderangeoflegumesspeciesisusedforforageproductioninsowngrasslands. Yet, despite their close phylogenetic relationship, theydisplay a wide range of growth habit, morphologies and competitiveabilities. Little is known about the elementary traits, which maketemperateforagelegumesdiffersosubstantiallyintheirgrowthhabit.Inthepresentstudy,wecomparedthepatternsofshootorganogenesisandof organ growthof six contrasting forage species (namely alfalfa,birdsfoot trefoil, sainfoin, Kura clover, red clover and white clover)duringtheirvegetativephase.Anexperimentwascarriedoutovertwoyears in a greenhouse under non-limiting water and soil nutrients.Phytomer initiation and shoot branching appeared driven bytemperatureandhighlydeterministic inall thespecies in theabsenceof competition for light. The temporal sequence of organ growthdiffered between species. However, organ growth was highlycoordinatedwithinaphytomerinallthespecies,andwasindependentofthepositionandaxisorderwhenexpressedinphyllochronictime.Bycontrast, organdimensions atmaturityweredependentonphytomerposition,andfollowedaregularfunctionoftherankforalltheorgans.Overall, a very similar developmental patternwas followed by all thespecies,buttheydifferedgreatly intheabsolutevaluestakenbytheirdevelopmentaltraits.

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PostersP85–S11 Conglutin proteins as biomarkers for the study of thebiochemical and physiological changes occurring duringlupinseedgerminationandseedlinggrowthRobles-BolivarP.1,AlcheJ.D.1,Jimenez-LopezJ.C.1,21-NationalCouncilforScientificResearch(CSIC)2 -EstacionExperimentaldelZaidin;DepartmentofBiochemistry,CellandMolecularBiologyofPlantsjosecarlos.jimenez@eez.csic.esNarrow-leafed lupin, NLL (Lupinus angustifolius L.) is mainlycharacterizedbyitsadaptationtodroughtstress,andtheabsenceofthe vernalization required for early flowering and maturity. Thislegumecrop ishighlybeneficial for agriculture i) actingas adiseasebreak in cropping rotations, and as control of grassweeds; ii) fixingnitrogen,andenrichingthesoilforsubsequentcrops.Despite the abundant knowledge about its agricultural traits, scarceinformationisknownabouttheNLLseedgerminationprocess.Inthepresentwork,wehavestudiedthedifferentstepsofNLLseedgerminationbymeansofmicrocopyandbiochemicalmethodologicalapproaches. A large amount of storage proteins is accumulated inprotein bodies (PBs), and mobilized during germination. We haveanalyzedtherolesofparticularconglutinsfamiliesofproteins(bandg) in the endosperm and cotyledon tissues, in order to betterunderstand the key molecular and physiological processes in NLLgermination,witha focusontheregulatorysignallingandmetabolicpathways after seed imbibition and until early stages of seedlinggrowth.The knowledge generated in this study provides evidences for thestructure-functional changes, and physiological tightly regulatedeventsoccurringduringgerminationintheNLLseedtissues.Acknowledgements:EUMarieCuriegrantref.PIOF-GA-2011-301550;the Spanish MINECO grant ref. RYC-2014-16536; and “Junta deAndalucia”fortheERDFgrantref.P2011-CVI-7487.

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P86–S11 Effectofmethanolonsomemorpho-physiologicaltraitsandyieldofchickpea(LensculinarisL.)PaknejadF.Amir moshiri Department of Agronomty, Karaj Branch, Islamic Azad University, Karaj,IranLentil, having a high protein (22 to 23 percent) in its seeds isconsidered as important source of vegetable protein. Methanol is asubstance that increases the CO2 fixation in plants tricarbonic.Methanol is relatively smaller comparedwith the CO2molecule thateasily by plants, are absorbed and used. In order to investigate theeffects ofmethanol on qualitative and quantitative traits of lentils indry conditions was conducted experiment in Khodabande 94-1393.Thisexperimentwasconductedinarandomizedcompleteblockdesignwith3replications.Thetreatmentsincludedcontrol,5,10,15,20and25percentbyvolumeofmethanol.Theresultsshowedthatmethanolsignificanteffectonthe lengthofbranches, leafdryweightperplant,numberofleaves,numberofpodsperplant,numberofseedsperpod,hundred seed weight, biological yield, grain yield, relative watercontentandcarotenoidcontentatalevelonepercentonplantheight,Numberofbranches,harvest index,chlorophyllcontentand leafareaindex at the level of five percent. The highest hundred-grain weightwasrelatedtotreatment,25percentbyvolumewith6.33gramsandthelowest5percentbyvolumetotreatmentwith3.96gr.25percentbyvolumemethanoltreatment.

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P87–S11 TheregulationofcarbonassimilationandnitrogenfixationduringnoduledevelopmentKhahluT.,VorsterJ.,TaylorN.University of Pretoria, Department of Plant and Soil Sciences, Forestry andAgriculturalBiotechnologyInstituteSoybeannodulesplayapivotalroleinfixingatmosphericnitrogenandusing that for their growth and development. Nodules have a shortlifespan, which limits the ability to fix nitrogen and affects overallgrowth.Thereisaneedtostudynoduledevelopmentandsenescenceto improvesoybeanplantphysiology.Of interest to this study, ishowcarbon assimilation and nitrogen fixation are affected during nodulesenescence.Thisisimportanttounderstandbecausetheknowledgeofhowthesetwoprocessesaffecteachotherisstilllimited.Twosoybeancultivars, Williams 82 and the commercial cultivar PHB 94Y80R(Pioneer) were used for the trial conducted at the Forestry andAgriculturalBiotechnologyInstitute(FABI).Plantsweresampledattimepoints: four, six, eight and ten weeks. Photosynthesis measurementsincludingA/Ciandlightresponsecurvesweretakentoquantifycarbonassimilation. The ureide assay was used to determine nitrogen fixingability of thenodules at said timepoints. To identify geneexpressionprofiles for the sucrose synthaseenzyme,RNAseqdata fromvanWyk(2015)wasused.Ninegeneswereidentifiedandeachhadatleasttwogene copies. The overall photosynthesis decreased from four to tenweeks, thesametrendwasmirroredbytheureideassayresults.Fourgenes namely:Glyma13g17421,Glyma17g05067,Glyma09g08550 andGlyma19g40041with the highest transcript numberswere chosen forfurtherexpressionstudieswithstudieswithRT-qPCR.

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P88–S11 EffectofsoilwaterdeficitonaminoacidexudationinPisumsativumrootsBobilleH.,CukierC.,RobinsR.J.,LimamiA.M.,FustecJ.URLEVA-ESA,UMRSESAN-IRHS,Univ.Angers,FranceAs part of rhizodeposition, root exudation may aid plants cope withabiotic stress by helping establish positive plant-soil interactions. But,relationships between plant physiology and exudated compounds arepoorlydocumented.Wehypothesizethataminoacid(AA)exudationbyrootsmay be quantitatively and qualitatively altered by water deficit(WD), as a physiological response of plants to stress. Pisum sativumplants grown inunsterilized soilwere stem-labeled (cotton-wick)with15N-urea for 72 h, then subjected or not to water deficit (WD). Theassimilation of 15N into AAs and their fingerprint were quantified byGC-MSinbothrhizosphereandroots.TherewasnosignificanteffectofWDoneitherdrymatterorNcontent intheplants.But,after24hofWDtheconcentrationofallAAsincreasedintheroots,accompaniedbya dramatic rise in isotopic enrichment. Furthermore, the release ofproline, alanine, glutamate and valine and the translocation of 15Nproline,15N-serine,15N-threonine,15N-glutamateand15N-asparagineinto the rhizospherewere increased significantly inWD.These resultssupport the idea that, under WD, recently assimilated N is rapidlytranslocated to the roots, and part of this is exudated. Serine andprolinearewellknowntobe involved in theplant response tostress,thelatterasanosmoprotectantofmacromolecules.Itcanbesuggestedthat the early exudation of prolinewould increase thewater holdingcapacitytofacilitatewateruptake.

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P90–S11Modellingtheeffectofassimilateavailabilityonseedweightandcompositioninoilseeds:thecaseofsoybeanBianculli,M.L.,Echarte,M.M.,Aguirrezábal,L.A.N.Laboratorio de Fisiología Vegetal, Unidad Integrada Balcarce (INTA-FCA, UNMdP),Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).laguirre@mdp.edu.arPods and seeds of species like soybean are green during theirdevelopment so that local effects of solar radiation on grainweightandcompositioncouldbeexpected.Onthecontrary,sunflowerseedsare non-green and the effects of solar radiation on weight andcomposition were exclusively mediated by assimilates produced byleaves.Theaimsofthisworkweretobuildageneralmodeltoexplaintheeffectsofsolarradiationonweightandcompositionofgrainsofoilseeds which differ in the photosynthetic capacity of theirreproductivestructures (e.g. soybeanandrapevs. sunflower)and intheircanopyarchitecture(e.g.soybeanvs.rapeandsunflower)andtoapplyittoaccountforexperimentaldata.Themodelwasbuiltbasedon results of field experiments where the amount of assimilatesupplied by vegetative structures and/or the amount of solarradiation reaching the podsweremodified.Weight, oil content andfatty acid composition of soybean seeds depend on assimilateavailabilitytotheirfilling.Localeffectsofsolarradiationreachingthepods also depend on the amount of assimilates coming fromvegetative organs. On the other hand, only the incident solarradiation on pods accounts for variation in seed weight andcompositioninrapewhiletheroleofleavescouldbedisregarded.Themodel was appropriated to describe soybean response to solarradiationandit ispotentiallyusefultoexplainthisresponseinotheroilseeds.

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P91–S11TheexpressionprofileofGAsignalingrepressor(LlDELLA)ingenerativeorgansofLupinusluteusMarciniakK.1,2,KućkoA.1,WilmowiczE.1,2,TretynA.1,2,KopcewiczJ.11-ChairofPlantPhysiologyandBiotechnology,2-CentreforModernInterdisciplinaryTechnologies,NicolausCopernicusUniversityinTorun,PolandGibberellins (GAs) control the proper development of generativeorgans in many plant species, including Lupinus luteus, the speciescultivated in Poland, Australia and many Mediterranean countries.Thekeyandnegative regulatorsofGA signalingareDELLAproteins.ComplexofGAwithreceptor(GID1)bindstotheDELLAsandleadstotheirdegradationviatheUPS.In this paper, the transcriptional activity of LlDELLA at differentdevelopmentstagesofinflorescence,flowersandpodsinL.luteusv.Taper was determined. During the development of the wholeinflorescenceaslightdecreaseofmRNAlevelwasobserved. Inturn,there were no significant differences in the content of the LlDELLAtranscripts in the six individual flower whorls of fully matureinflorescence.Followingsenescenceandwiltingofflowers(withsmallinitially formed pods) and pods development, rapid decrease in thetranscriptional activity of investigated gene was recorded. In plantswithfullymaturepods ineachwhorl,weobservedapproximately3-fold lower level of gene expression compared to plants with fullydevelopedflowers.The profile of LlDELLA gene expression may be correlated with GAinvolvement in appropriate generative organs development. In thenear futureour resultswilldetermine theprecisemechanismof theflowerandpoddevelopmentofL.luteus.ThisresearchwasfundedbytheprogramsupportedbyResolutionoftheCouncilofMinistersofPoland(RM-111-222-15)"

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Session 12, parallel: Integrated pest and diseasemanagement-RoomArrábidaIVChaired by Jenny Davidson (PIRSA-SARDI, Australia) andChristopheLeMay(INRA,France)16:30-17:00 Key lecture: Seid Kemal: Integrated diseaseand insectpestmanagementpest and in cool-season foodlegumes

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OralCommunications17:00-17:10 Oral–S12PISOM(Ideotypes,Systems,SurveysofPeaandfababeanMaindiseases)BarangerA.1,3,BoutetG.1,3,BrierC.2,3,LeMayC.1,3,Pilet-NayelM-L.1,3,MoussartA.2,31-UMRIGEPP,INRA,AgrocampusOuest,UniversitéRennes1,35650LeRheu,France2-TerresInovia,AvenueLucienBretignières,78850ThivervalGrignon,France3-UMTPISOM,TerresInovia-INRA,35650LeRheu,FranceThe French Technological Mixed Unit PISOM is a partnership toolbetween INRA, the National Institute for Agronomic Research andTerres Inovia, the technical institute for grain legumes. It aims atdevelopingknowledgeandtoolstodescribe,predictandmanagemainpeaandfababeandiseases.Amongconsideredissuesistheimpactofclimateandsystemschangeson diseases incidence and severity at the regional or national scales.Surveysemphasizetheeffectsofenvironmentalconditionsandculturalpracticesontheemergenceandseverityofdiseases.Anotherpurposeis thedesignof ideotypes tocontroldiseasesusing individualplantorcanopy traits, such as partial resistance, tolerance, architecture andphenologycharacters.Elucidationofthegeneticcontrolofthesetraits,and identification of linked molecular markers to trace them inbreeding, open the way to more efficient and durable epidemicscontrol. A third objective is to anticipate new cultural systems forsustainable diseasemanagement. This includes new culturalmethodsandrotations,andcombiningpartiallyefficientmethods.PISOM invests in increasing the speed and quality of the researchresults transfer tostakeholdersbysupplyingbreeding lines,molecularmarkers, methodologies and cultural recommendations. Prospectsinclude a better vision of emerging diseases and diseases riskassessmentwitharangeofstrategiesbasedonspeciesandplanttraits,andtheirdeploymentinagriculturalsystems.

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17:10-17:20Oral–S12TheAustralianAscochytarabieipopulationstructureandevidenceofahighlyadaptedandevolvinghaplotypeMehmoodY.1,SambasivamP.2,FordR.1,21-SchoolofNaturalSciences,GriffithUniversity,Queensland,Australia4111.2-FacultyofVeterinaryandAgriculturalSciences,TheUniversityofMelbourne,Victoria,Australia3010TheAustralianAscochyta rabiei populationhaspreviouslybeen foundto be genetically narrow with only one mating type gene detected,potentiallyprecludingevolutionthroughrecombination.Inanefforttobetterunderstandtherisktocurrentlyusedresistancesourcesthroughselectiveadaptation,thepopulationwasexaminedingreaterdetail.Forthis,atotalof416isolateswerehierarchicallycollectedfromwithinthemajorAustralianchickpeagrowingregionsandfromcommonlygrowncultivarsin2013and2014.GenotypingviaSSRlociindicatedanoveralllowdiversity(0.074),aspreviouslyobserved.Althoughalargenumberof haplotypes were detected (78), more than 64% of the populationbelonged to a single dominant haplotype (ARH01). Pathogenicscreeningonadifferentialhostset,ICC3996(R),cv.Genesis090(R),cv.PBAHatTrick(MR)andcv.Kyabra(S),revealeddistinctisolatepathotypegroups,with17%ofallisolatesassessedidentifiedashighlyaggressive.Of these, over 63.33% belonged to the ARH01 haplotype. A similarpattern was observed at the host level, with 46% of all isolatescollectedfromGenesis090(R) identifiedashighlyaggressive,ofwhich50%belonged to theARH01haplotype.This is veryhighcompared tothepercentageofaggressive isolatesdetectedwithinotherhaplotypegroups i.e. ARH125 (6% aggressive isolates) and ARH144 (3.33%aggressive isolates).This indicates the fitnessofARH01tosurviveandreplicateonourbestresistancesources.

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17:20-17:30Oral–S12Chickpeadamping-offcausedbymetalaxylresistantPythiumintheUSPacificNorthwestChenW.1,McGeeR.1,PaulitzT.1,PorterL.1,VandemarkG.1,GuyS.2,SchroederK.31-USDA-ARS,PullmanandProsser,WA;2-WashingtonStateUniversity,Pullman,WA;3-UniversityofIdaho,Moscow,ID,USAChickpea seed rot and damping-off is an important diseaseworldwide.Metalaxyl isanactiveingredientinfungicidesthathavebeen used for decades to control plant diseases caused byOomycetes,suchasPythiumandPhytophthoradiseases,andusedinseed treatments to protect seeds from Pythium damping off. In2014andagainin2015and2016,wehaveobservedthatmetalaxyl-treatedchickpeaseedsfailedtogerminateinadozenorsofieldsintheUSPacificNorthwest, requiring reseeding in several fields. Theun-germinated seeds become soft, slimy, and coated by soilparticles, and are often difficult to find because they just look likethe soil. Pythium isolates obtained from rotten seeds are highlyresistant to metalaxyl. Additionally, soils sampled from the areaswhere there was poor germination consistently showed Pythiumpopulations with high levels of resistance to metalaxyl. Undercontrolled conditions, metalaxyl treatments failed to protectchickpea seeds from damping-off inoculated with metalaxyl-resistant Pythium isolates. All evidence shows that the poorgermination of Kabuli chickpea is due to the Pythium populationsresistanttometalaxyl.Weareworkingtodeterminefieldprevalenceof metalaxyl-resistance in chickpea production areas, identifyalternative fungicides that can control or manage metalaxyl-resistant Pythium, and investigate mechanisms of metalaxylresistanceinPythium.

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PostersP92–S12 TheincidenceofdiseasesandpestsoffababeanondifferentvarietiesaccordingtodifferentlocationsOlleM.EstonianCropResearchInstitute,EstoniaThe incidence and diversity of diseases and pests are changing indecades.Climatechangeresultsareconsequentadverseeffects, likeincreasedfrequenciesofabioticstresses(heat,drought)andincreaseofbioticstresses(pestsanddiseases).Thereforetheaimofthestudywas to evaluate the diseases and pests incidence on different fababean varieties in three different locations: Pūre (Pūre HorticulturalResearchCentre; Latvia), Priekuļi (InstituteofAgricultural ResourcesandEconomics;Latvia)andJogeva(EstonianCropResearchInstitute,Estonia).TheincidenceofAphids(Aphisspp.),Seedweevils(Bruchusspp.), Chocolate spot (Botrytis fabae), Ascochyta blight (Ascochytafabae), Rust (Uromyces fabae), Stem rot (Sclerotinia spp.) and FabaBeanYellowMosaicViruswerescored.Varietiesoffababeanusedinthe trial: Gloria, Julia, Jogeva, Lielplatones and Bauska. The resultsshowedthatoverall fromvarieties themostsusceptible fordiseasesin different locations was Jogeva, followed by Gloria. The lesssusceptible varieties were Lielplatones and Bauska. However thedistributionof diseases andpests differedbetween locations and indifferent years. In 2014 the disease damages were increasedcomparedto2015,probablybecauseofdifferentweatherconditions.In Priekuļi onlyAscochytablightwaspresent in notablepercentage,while in Pūre and Jogeva mostly chocolate spot and seed weevildamagedplants.

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P93–S12 TherelationshipbetweensowingdateofViciafaba(fieldbeans)andthelevelofdamagecausedbyBruchusrufimanus(beanseedbeetle)intheUKWardR.L.1andBrownP.21-ProcessorsandGrowersResearchOrganisation,TheGreatNorthRoad,Thornhaugh,Peterborough,PE86HJ.2-FrontierAgricultureLtd.,SandyLane,Diss,Norfolk,IP224HY.The bean seed beetle, Bruchus rufimanus (Boheman), is aneconomically important pest of Vicia faba bean crops throughoutEurope and causes severe quality losses due to seed and producedamage. The beetle has one generation per year and insects emergefrom over-wintering in the UK in April andMay to feed in floweringbean crops. The females lay eggs on the outside of developing pods,predominantlyatthebaseoftheplantwherepod-setstarts.Hatchinglarvaeborethroughthepodwallsanddevelopwithintheseed.Whenfully grown, larvaepupate and young adult beetles emerge, leaving aroundholeinthebean.FollowingevaluationofdatacollectedfromUKfield bean crop harvest samples between 2006 and 2014, and cropdevelopmentevaluationsatanumberoffieldsites,apatternemergedthatindicatedarelationshipbetweenthedateoffirstpodset,andtheamountofdamagecausedbythepest.Aseriesoftrialssownatthreedifferent timings was established in 2015 to further investigate thisrelationship. The results showed that damage reduction to harvestedproducewasupto50%whendrillingwasdelayed.Thishasprovidedavaluable addition toUK recommendations for control of thepest andmay allow growers to reduce insecticide applications in field beansduringthecriticalfloweringandpodsetperiod.

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P94–S12 EmploymentofvarietalmixturestoreducetheincidenceofdiseasesongrainlegumesVillegas-FernándezÁ.M.,MoralJ.,RubialesDInstituteforSustainableAgriculture,CSIC,Apdo.4084,14080,Córdoba,SpainThe employment of resistant varieties is one of the best options tocontroldiseases in legumes,both inecological andeconomical terms.Nevertheless, one of the problems associated to this practice is theappearance of pathogen strains that are able to overcome the plantresistance. This calls for an efficient and wise management ofresistancegenesinwhichdiversificationisalwayskey.Wehavefocusedontheuseofvarietalmixturestoreducetheimpactof diseases in two important legume crops: pea and faba bean. Twofieldexperimentswerecarriedoutforeachcrop,theassesseddiseasesbeing powderymildew in pea and rust in faba bean. In both cases, aresistant monogenic variety was mixed with a susceptible one. Fivetreatmentswere tested, corresponding to growing proportions of theresistant variety: 0%,25%,50%,75%and100% (rowsof the resistantvariety were alternated with varying numbers of rows of thesusceptibleonedependingonthedesiredproportion).Diseaseseveritywasrecordedonthesusceptiblevarietyineachtrial.Results in both experiments show a significant negative correlationbetweendiseaseseverityonthesusceptiblevarietyandtheproportionoftheresistantone.Severityreductionisimportantwhentheresistantvariety is grown every other row (50% proportion), reaching highestlevelswith75%proportion.Thedilutioneffectof inoculumduetothedistancebetweensusceptibleplantsisprobablybehindtheseresults.

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P95–S12 DiversityofascochytablightdiseasecomplexoffieldpeaandsoybeaninserbiaPetrovićK.1,RiccioniL.2,ValenteM.T.2,ĐorđevićV.1,ŽivanovD.1,VidićM.1,MiladinovićJ.11-InstituteofFieldandVegetableCrops,21000NoviSad,Serbia.2-Consiglioperlaricercainagricolturael’analisidell’economicaagraria-Centrodiricercaperlapatologiavegetale,I-00156Rome,ItalySoybean and field pea are the most important legumes worldwide.However, diseases are a strong limitation to obtaining stable yields,amongwhich Ascochyta blight complex is one of themost significantandwidespread pathogens. Three causal agents of pea diseases havebeen described: Peyronellaea pinodes, the most common and mostdamagingcausalagentofblight;P.pinodella, thecausalagentof footrot; andAscochyta pisi, causal agent of blight and pod spot. OnlyA.sojaecola is reported as causal agent of leaf spot on soybean.Taxonomy of Peyronellaea and Ascochyta species was the subject ofmany studies for a long time. Initial identification was based onmorphological characteristics,which isdifficultbecausemorphologicalfeaturesoftenvaryand"overlap",makingitimpossibletoestablishtheappropriate taxonomic rank. Taxonomy of these species is currentlyredefinedmostlybyusingDNAsequences.Basedonthemorphologicalcharacteristics, onlyA. pisi on pea andA. sojaecola on soybeanweredetermined in Serbia, but the identificationwasnot confirmedat themolecular level. The objectives of this study were to identifyPeyronellaea and Ascochyta species that are found on field pea andsoybean in Serbia, and provide a clearmorphological profile for eachspeciesfollowedbymolecularidentificationandpathogenicitytest.Thefirst results indicated the presence of several new species associatedwithAscochytablightcomplexonpeaandsoybeaninSerbia.

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P96–S12 Plantdiseasecomplex:didantagonismandsynergismbetweenpathogensbetheresultofdifferentiatedlifetraits?LeMayC.Agrocampus,INRA,DomainedelaMotteBP35327,LeRheu,35653,FranceInadiseasecomplex,differentpathogenspeciescommonlydevelopsimultaneously in a field and can then infect the same hostindividuals.Co-occurringpathogensmayaffecteachother,throughantagonism and/or synergism. An important question is theecological and pathological consequences of co-occurrence ofpathogenic species within a disease complex. By using twopathogens (Didymella pinodes (Dp) and Phoma medicaginis var.pinodella(Pm))oftheAscochytablightcomplex,thisstudyaimedatdescribing how co-occurrence affects the development ofpathogens and disease severity. One of the main principles ofecology is competitive exclusion, the hypothesis given for explainthis simultaneous presence is the niche differentiation byseparation in time. Inorder toprovethishypothesis, thepresenceofa trade-offbetweentheparasitic fitnessandsaprophytic fitnesswasalsoevaluated.Resultsindicatedalowsaprophyticfitnessthatwould be offset by a higher parasitic fitness (aggressiveness,reproduction) for Dp while Pm seemed to invest more in thesaprophytic fitness (survival, chlamydospores). Moreover, thepresence of the two pathogens on the same host plant organlimited thediseasedevelopment and their reproduction.Damagescausedbythetwopathogens,however,increasedwhenplantsthathad been previously inoculated were inoculated with the otherspecies.Thedevelopmentofonepathogenspeciescanbeaffectedbyasubsequentpathogeninoculation.

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P97–S12 EffectofpeasowingdateonaphanomycesrootrotdevelopmentandyieldlossesMoussartA.1,2,3,EvenM.N.1,2,3andBarangerA.2,31-TerresInovia,AvenueLucienBretignières,78850ThivervalGrignon,France2-UMRIGEPP,INRA,AgrocampusOuest,UniversitéRennes1,35650LeRheu,France3-UMTPISOM,TerresInovia-INRA,35650LeRheu,FranceSuccessive sowings of a susceptible pea cultivar were realized fromOctober to April, in a highly infested field and were subjected toaphanomyces root rot severity assessments until maturity. Firstsymptoms on roots appeared at later plant stages in autumn-sownthaninspring-sownplantsduetomoreconduciveclimaticconditionstothediseasefromearlyspring.The effect of inoculation of a susceptible pea cultivar at differentplant stagesonsubsequentplantgrowthandyieldcomponentswasthenassessedinthegreenhouse.Theyoungertheplantswereatthetime of infection, the more damaging the disease was on yieldcomponents.Contaminationafterfloweringhadnoimpactongrowthandyieldcomponents.The effect of sowing dates (autumn-sown vs spring-sown) on yieldlosseswasconfirmedinalargefieldnetwork(eachsitecomprisingavery infested and a healthy neighbouring fields). Autumn-sownshowed no aerial symptoms and no or below 15% yield losses,whereas spring-sown always showed typical aerial symptoms(yellowingandstunting)andhigheryieldlosses(from10to68%).Theloweraphanomycesrootrotimpactinautumn-sownpea,duetothemoreadvancedgrowthstageatthetimeofcontamination,makeswinterpeaanalternativetospringpeainmoderatelyinfestedfields.To secure yield and avoid pathogen multiplication, it is howeveradvised not to cultivatewinter pea in heavily (inoculum potential >2.5)infestedfields.

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P98–S12 NecrotrophiceffectorsproducedbyfungalpathogensoflegumecropsCimminoA.1,*FondevillaS.2,MasiM.1,Villegas-FernándezA.M.2,RubialesD.2,EvidenteA.31 - Dipartimento di Scienze Chimiche, Università di Napoli Federico II, ComplessoUniversitarioMonteSant’Angelo,ViaCintia4,80126,Napoli,Italy.2-InstituteforSustainableAgriculture,CSIC,14004Córdoba,Spain.3 - Dipartimento di Scienze Chimiche, Università di Napoli Federico II, ComplessoUniversitarioMonteSant’Angelo,ViaCintia4,80126,Napoli,Italy.Despitetheir importancefortheagricultureandtheenvironment,theproductionoffoodlegumesisdecreasinginmostoftheMediterraneanfarmingsystems.Amajorcauseforthisisthelowandirregularyieldasa consequence of biotic and abiotic stresses. Necrotrophic fungi,including essentially Ascochyta and Botrytis species, are among themainbioticconstraints.Bothfungalgenusarewellknownphytotoxinsproducers. These toxins, belonging to different classes of naturalcompounds, are frequently involved in the development of diseasesymptoms.In this communication the chemical and biological characterization ofthenecrotrophiceffectorsproducedbyDidymellapinodesandBotrytisfabae pathogens of pea and faba bean respectively,will be reported.Furthermore, thestudiesaimedto identifynovelresistancesources intherespectivehostsinsensitivityassayswillbediscussed,too.

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P99–S12 BiocontrolofemerginginsectpestsoflegumecropsEvidenteA.1,Aznar-FernándezT.2,MasiM.1,RubialesD.2,CimminoA.11 - Dipartimento di Scienze Chimiche, Università di Napoli Federico II, ComplessoUniversitarioMonteSant’Angelo,ViaCintia4,80126,Napoli,Italy.2-InstituteforSustainableAgriculture,CSIC,14004Córdoba,Spain.Pea weevils (Bruchus pisorum) and pea aphids (Acyrthosiphon pisum)are insect pests of great economic importance for agriculture andrepresent one major cause of damage and loss in legume crops.Bruchuspisorumisspecialist,feedinganddevelopingalmostexclusivelyon pea. Acyrthosiphon pisum feeds on several species of legumesworldwideandranksamongtheaphidspeciesinagriculture.Recently,germplasmcollectionsofpeahavebeenscreenedunderfieldconditions to identify sources of resistance to weevils and aphids inpea.The identification of compounds with phagodeterrent activity, whichare able to interfere with aphid host plant selection and hostacceptance, is currently becoming of great interest for the design ofinnovativebiotechnicalstrategiesincontrolofphytophagousinsects.In this communication results obtained on the identification ofmetabolites responsible for antixenosis and/or antibiosis in pearesistantaccessions toweevilsandaphidswillbereported.Theeffectofnaturalmetabolitesagainstthesepestswillbediscussed,too.

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P100–S12 TowardtheunderstandingofhostdifferentiationofOrobanchecrenatapopulationsonlegumesMentagR.INRA-CentreRégionaldeRabat,Rabat,Morocco.Orobanche crenata representsamajorbiotic constraint toproductionoffababean(ViciafabaL.)andlentil(LensculinarisMedik.).Whilethisparasiticplantspeciesattacksbothofthesecrops,theextenttowhichO.crenatabiotypesspecializeinparasitizingspecificcropsisunknown.Toaddressthisquestion,westudiednaturalpopulationsofO.crenataseedproducedondifferenthostsandquantifiedtheirhostspecificitytofababeanand lentil. ThevirulenceofO. crenata populationsoneachhostwas investigated through field trials,potandPetridishesassays.Geneticdiversityoftheparasitepopulationswasalsoassessedthroughmolecular analyses. Evaluation under controlled conditions showed agreater affinity between host species and their associatedO. crenatapopulations.Thetwo legumehostspeciesshoweddistinctpatternsofinfestation. Faba bean was more sensitive to both O. crenatapopulations, while the specificity for lentil by lentil-grownO. crenatawas evident by the final stage of the parasite life cycle as shown byCorrespondence Factorial Analyses. Considerable internal variation(81%)withinO. crenata populations growing on both legume specieswasalsoobserved.Theseresults indicatethatO.crenatacanadapttospecific host species,which is important knowledgewhen developingintegratedpestmanagementpracticesforparasiticweed.

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P101–S12 GeneticandphenotypicdiversityofpeaisolatesofAphanomyceseuteichesinFranceQuillévéré-HamardA.1,2,LeRoyG.1,2,MoussartA.2,3,Pilet-NayelM.-L.1,2,LeMayC.1,21-INRA-AgrocampusOuest-UniversitéRennes1,UMR1349IGEPP,LeRheuF-35653,France2 - UMT PISOM INRA/Terres Inovia, Le Rheu F-35653, France 3 Terres Inovia,ThivervalGrignonF-78850,FranceAphanomyces euteiches is an oomycete pathogen that causesdevastatingrootrotinmanypea-growingcountries.Croprotationswith resistant or non-host legumes and genetic resistance are themain ways to manage the disease, but require more knowledgeabout structuration of the pathogen population. The aim of thisstudy was to evaluate the genetic and phenotypic diversity of A.euteiches isolates in France. A collection of 207 isolates wasestablished from pea-infested fields located in eight Frenchdepartments. The isolateswere genotyped using 20 SSRsmarkers(LeMayetal,2016)anda subsetof34 isolateswasevaluated foraggressivenessona setof legumes (pea, fababean, vetch, alfalfa)(Moussartetal.,2008).ResultsshowedlowgeneticandphenotypicdiversitybetweenmostofA.euteichesisolatesandnogeographicalstructuration. However, some isolates with specific molecularpatternsweredetectedinonelocationwithdifferentcrophistoriesin legumes. Most of the isolates were aggressive on all the fourlegumes tested but some of them showed low aggressivenessdepending on the host. These results suggest low genetic flowsbetweenA.euteichespopulations,inaccordancewiththebiologyofthepathogen.TheyopennewprospectsforinvestigatingtheroleofhostdiversityonstructurationofA.euteichespopulations.

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P102–S12Effectofintercroppingfieldpeawithspringcerealsontemporalchangesinpeaaphid(AcyrthosiphonpisumHarris,1776)abundancesanddistributionincropsSeidenglanzM.1,HuňadyI.2,ŠafářJ.2,Villegas-FernándezA.M.31 - AGRITEC Research, Breeding and Services, Ltd., Department of Plant Protection,Zemědělská2520/16,Šumperk,78701;CzechRepublic2 - Agritec Plant Research Ltd., Department of Grain Legumes and Technical Crops,Zemědělská2520/16,Šumperk,78701;CzechRepublic3-CSIC/IASInstitutodeAgriculturaSostenible,AvenidaMenéndezPidals/n,CampusAlamedadelObispo,Córdoba,14004;SpainInthecourseof2013-2015abundancesofpeaaphid(A.pisum)andtheir natural enemies found on field pea plants, grown either as amonoculture or intercropped with spring cereals and some otherlegumes,were compared. Two types of trialswere used: small plottrials and large plot trials. Pea aphid colonies in field peasintercropped with cereals developed differently than in peamonocrops.However,thesmallplottrialsshoweddifferentresultstothelargeplottrials.Inthesmallplottrialsonintercroppedplotspeaaphidnumberswerehigherduringthefirstpartofcropcolonisation,but the decline in aphid populations also happened earlier. In thelargeplottrialspeaaphidabundancesweresignificantlylowerinfieldpea – barley intercrops compared to monocrops during the wholedurationofcropinfestation.Peaaphidcoloniesencounteredsyrphidlarvaeattacksmorefrequentlyinintercropsthaninmonocrops.

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P103–S12 DeterminationoffungicideefficacyonrustoffieldpeaMarkellS.1,HalvorsonJ.1,BerghuisB.1,HumannR.1,MeyerS.1,SchuhC.1,HaugenS.1,JordahlJ.1,FittererS.2,CarruthD.2.1-DepartmentofPlantPathologyNorthDakotaStateUniversityFargo,ND58102U.S.A2-BASFNorthDakotaResearchFarmDavenport,ND58021U.S.A.Dryediblepea(Pisumsativum)acreageinthetwoUnitedStatesnorthcentral statesofMontanaandNorthDakotahas recently approached0.5 M Ha. Reports of rust, caused by Uromyces viciae-fabae (Pers.)Schroet., have recently increased in the region and are cause forconcern. The objective of this study was to evaluate the efficacy ofnumerous fungicides for the management of rust on field pea.FungicidetrialswereconductedinLeonardandFargo,NDin2015and2016.All trialswere arranged in a randomized completeblockdesignwith four replications, and included a varying number of treatmentswithdifferentmodesofaction,including;QoI(FRAC11),DMI(FRAC3)and SDHI (FRAC 7). Trials were artificially inoculated with fresh U.viciae-fabae urediniospores and fungicides were applied after theoccurrence of disease. Disease severity was determined visually byevaluatingthepercentofthecanopycoveredwithpustulesineachplotand/or the percent leaf area coveredwith pustules on ten arbitrarilyselected plants within each plot. In 2015, disease severity in plotstreated with Azoxystrobin, Pyraclostrobin, Prothioconazole, andFluxapyroxad + Pyraclostrobin was statistically lower than the non-treated control plot in at least one location. In 2016, the number oftreatmentsevaluatedforefficacyonrusthasbeenexpandedandyielddatawillbetaken,ifpossible.

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Session 13, plenary: Frontiers in legume breeding -RoomArrábidaI&IIChaired by Wolfgang Link (Georg-August-University,Germany)andGerardDuc(INRA,France)08:30-09:00 Key lecture - Scott Jackson: Contribution ofepigeneticvariationtoimprovement

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OralCommunications09:00-09:15Oral–S13QTLdetectionforforagebiomassofalfalfainmixturewithaforagegrassJulierB.INRA,LeChêne-RD150CS80006,Lusignan,FranceThe agronomic and environmental value of forage grass - legumemixtureshasbeenprovedbutlegumebreedingisstillmostlyconductedin monoculture. A progeny of 200 F1 individuals obtained from twoalfalfa parents (H1 and G4) contrasting by aerial morphology wasevaluatedforbiomassandplantheightundertwocroppingconditionsin micro-sward (mixture with tall fescue and monoculture) for 8consecutive cuts. Phenotypic data showed a large variation amongindividuals for all traits in all cuts. The correlation between traitsrecorded in mixture and in monoculture was positive but the largevariation around the correlation indicated that some genotypeswererelatively more performant either in mixture or in monoculture. AgeneticmapofeachparentwasobtainedfromSSRandDArTmarkers,byusingtheTetraploidMapsoftware.QTLwerefoundoneachparentandeach linkagegroup (LG).MostQTLwere common tomixtureandmonoculture (LG 1 of H1; LG 1, 2 and 4 of G4) but some QTL werespecifictothemixturecondition(LG3and7ofH1,LG6ofG4).Theseresults indicate a partial common genetic control for biomass inmixture andmonoculture but also highlight a specific genetic controlfor performance in mixture. To create alfalfa varieties adapted tomonocultureandmixedcroppingconditions,bothcommonandspecificQTLforbiomasscouldbeused.

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09:15-09:30 Oral–S13Improvingtheresistanceoflegumecropstocombinedabioticandbioticstress(ABSTRESS)CharltonA.J.FeraScienceLtd,NationalAgrifoodInnovationCampus,SandHutton,YorkYO411LZ,UKCropvarietiesthatarebetterabletotoleratetheeffectsofclimatechange are key to a sustainable future for European legumefarming. Modern phenotyping tools and more diverse germplasmarerequiredtoensureefficientcropbreeding.Combined integrated systems biology and comparative genomicsapproaches were developed to conduct a study of the genenetworks and metabolic pathways implicated in the interactionbetween drought stress and Fusarium oxysporum infection inMedicago truncatula. Plants subjected to combined stress wereproducedusingahigh throughputphenotypingplatformequippedwithmolecularimagingtechnologies.Bayesianmodelswereappliedto metabolomics and transcriptomics data derived from M.truncatula to identify “hub” genes and key control points inmetabolic pathways implicated in combined drought and diseaseresponse.Thirty-six hub gene candidates were identified in M. truncatula.Genetic mapping identified 28 hub gene orthologs in pea. Thesehave a similar gene expression profile inM. truncatula and peawhen exposed to combined stresses. Using gene silencingtechnologies, these hub genes have also been demonstrated tocorrelate with a phenotypic response. Tilling and eco-tilling formutants of the 28 pea genes is producing new germplasm fortestinginfieldtrialsandultimatelybreedingresistancetocombinedstress. Introgression and multiplication of the mutant seeds iscurrentlybeingcompletedwithfieldperformancetrialsplannedfor2017.

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09:30-09:45 Oral–S13IntegratedplatformforrapidgeneticgainintemperategrainlegumesandwildCicerspeciesPazos-NavarroM.,RibaltaF.,TschirrenS.,MundayC.,EdwardsK.,BennettR.,WellsS.,ErskineW.,CroserJ.CentreforPlantGeneticsandBreeding,TheUniversityofWesternAustralia,35StirlingHwy,Crawley,WA6009,AustraliaPulse genetic improvement has historically been constrained by thelong lifecycle of the plant resulting in slow genetic gain. Inconventional breeding systems, one to three generations can beenobtained inasingleyear,withsix requiredfor fixationof favourablegenes. Our research has resulted in breakthrough technology toaccelerate the traditional Single Seed Descent (SSD) system in fivemajorgrainlegumes(chickpea,lentil,lupin,peaandfababean)andinwild Cicer species as C. echinospermum and C. reticulatum.Accelerated SSD (aSSD) enables a turnover of 6-8 generations peryear, more than halving the current fastest route (SSD) tohomozygosityinthesespecies.Keytothissystemis:(1)Plantgrowthunder tightly-controlled environmental and physical conditions torapidly initiateflowering;and(2)Aworld-firstsystemforprecociousgermination without in vitro intervention. With the final goal ofdeveloping an integrated breeding platform, we have designedhydroponic selection screens (HSS) for key abiotic constraints,including salinity, aluminium and boron toxicity that can be fullyintegrated through the aSSD system. This breeding platformrepresentsastep-changeinthepotentialefficiencyofplantbreedingprograms. The proposed technology will benefit complex geneticstudies via the rapid development of recombinant inbred lines (RIL)and multi-parental advanced generation intercrosses (MAGIC)populations."

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09:45-10:00 Oral–S13BroadeningthegeneticbaseoflentilSarkerA.1,NathR.2,AliO.31-ICARDA,India2-BCKV,India3-BARI,BangladeshLentil (Lens culinarisMedikus subsp. culinaris) is an important pulsecrop grown and consumed in South Asia since time immemorial. Itsseed is nutritious human food, plant residues are valued animal feedand its cultivationprovides sustainable cereal-basedcropping systemsin the region. Lentils in South Asia have narrow genetic base withrespect tomorphological, phenological and stress tolerant traits. TheInternationalCenterforAgriculturalResearchintheDryAreas(ICARDA)and its partners in South Asia are engaged in broadening the geneticbaseoflentilwiththeaimtodevelophighyieldingvarietiessuitableforcultivation under various crop production systems. ICARDA holds thelargest collection of > 11,000 diverse lentil germplasm from 72countries, which is the building block of an international breedingprogram. Targeted utilization of these materials in geneticenhancement research has resulted into construction of newgenotypes, and development of improved cultivars in Bangladesh,India,NepalandPakistan.Someof thesevarietieshaveearly seedlingvigor, early maturity, robust root systems, high biomass, and haveability to adapt under mixed cropping, inter-cropping and no-tillproduction systems. Additionally, high iron and zinc content varietieshavebeendeveloped,super-earlylentilgenotypeshavebeenidentifiedtofitintorice-lentil-ricecroppingsystem,andforsuccessfulcultivationinrice-fallowsasasecondcrop.

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PostersP105–S13 ChallengesandprogressinimprovinggeneticresistancetomycosphaerellablightoffieldpeabyconventionalbreedingBingD.AgricultureandAgri-FoodCanada,CanadaMycosphaerella blight, caused by Mycosphaerella pinodes (Berk. &Blox.) Vestergr., is the most prevalent disease of field pea (PisumsativumL.)worldwide.Todateextensiveresearchhasnotbeenabletoidentifyanystronggeneticresistancetothedisease.Variousbreedingtechniques applied to improving the resistance, progress andchallenges over 10 years in the field pea breeding program atAgricultureandAgri-FoodCanadaarereviewed.

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P106–S13 HayandseedyieldperformancesofViciasativasubsp.sativaXViciasativasubsp.macrocarpahybridsinF4generationAcikgozE.UludagUniversity,FacultyofAgriculture,Bursa,Turkey.TheViciasativaaggregate isacomplexofsixcloselyrelatedtaxawhichcombinescultivated,weedyandwildforms.Commonvetch(Vicia sativa subsp. sativa L.) is widely grown for hay and seedproductionintheMediterraneanbasin.Bigleafvetch(Viciasativasubsp.macrocarpa(Moris)Arcang.)isusuallyfoundinthewetterareasof theMediterraneanregion. It is latematuringsubspecieswith very large seeds and leaflets. Twowhite flowered commonvetch accessions (W-1 and Soner)were crossedwith two purpleflowered big leaf vetch accessions (Ericek and ICARDA – 5283)underthegreenhouseconditionsin2012.Hybrids between the subsp. sativa x subsp. macrocarpa werehighlysterile.F1andF2generationsshowedextremlylowfertilityinall combinations.But restorationof fertilityoccurred in theF3generation and several lines were developed. The lines wereevaluated under dryland conditions in a randomized completeblock design with 3 replications. Large morphological variationswereobservedamongtheF4lines.Somehybridsweresuperiorinmostyieldcomponents,biomassandseedyieldthanbothparentsandthecheckcultivars.

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P107–S13 Generationoflinkedmarkersforlowvicine-convicine(lvc)andzerotannin(zt-1)traitsandtheiruseinMarkerAssistedSelection(MAS)KissG.B.1,WinklerJ.2,AdamE.2,FerrariB.3andPaoloAnnicchiarico31-AMBISLtd,Szeged,Hungary.2-SaatzuchtGleisdorfGmbH,Austria.3-CRA-FLC,Lodi,Italy.

PolymorphicPCRbasedgeneticmarkerstightlylinkedtolowvicineconvicinecontent (lcv)andzerotannin (zt-1)traitsweredevelopedforseveralfababean(Viciafaba)parents. These markers (VfVc12, VfVc13, VfZt12) wereused to genotype individuals in several breedingpopulations to help marker assited selection for thesetraits. Results of the genotiping and evaluation of theresultswillbepresented.

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P108–S13 Modelswithtwopredictorvariablesaccuratelypredictsseedyieldindiploidandtetraploidredclover(Trifolium

pratense)VleugelsT.1,CeuppensB.1,3,SmaggheG.3,Roldán-RuizI.2,CnopsG.21 - Plant SciencesUnit –Genetics andBreeding, Institute for Agricultural and FisheriesResearch(ILVO),Caritasstraat39,9090Melle,Belgium.2-PlantSciencesUnit–GrowthandDevelopment,InstituteforAgriculturalandFisheriesResearch(ILVO),Caritasstraat39,9090Melle,Belgium.3-GhentUniversity,FacultyofBioscienceEngineering,Dept.ofCropProtection,CoupureLinks653,9000Ghent,Belgium.Redclover(TrifoliumpratenseL.)isavaluableprotein-richforagecrop.Tetraploid red clover cultivars produce more dry matter weight andhaveincreaseddiseaseresistancecomparedtodiploidcultivarsandaretherefore favoured by farmers. However, the disadvantage oftetraploidcultivars is theirunsatisfactoryseedyield,whatmakesseedproductionoftetraploidseconomicallyuninteresting.We investigated the effects of 10 traits on seed yield in a set of 600individual genotypes, derived from 15 diploid and 15 tetraploidcultivars.Ourdataandmodelsshowtheimportanceofonlytwotraitsforthedeterminationofthevariationinseednumber.Thenumberofripeflowerheadsperplantandtheseednumberperripeflowerheadexplained respectively 94.8% and 53.2% of the variation in diploidplants,and88.1%and64.9% in tetraploidplants.The latter twotraitscanbe included in thebreedingprogramto increaseseedyield in redclover.

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P109–S13 DiversewinterfababeansinmixedcropstandwithwheatSiebrechtD.,MartschR.,LinkW.Georg-August-Universität Göttingen, Department of Crop Sciences, Division of PlantBreedingMixedcropstandscanshowhigheryieldswhencomparedwithpurestands. Such yield increments are assumed to result fromcomplementarityeffectsbetweencrops.Inparticular,itisarguedthatmixedcropstandsoflegumesandnon-legumeshavethepotentialtothe promote sustainability and resilience of our food and feedproduction.One such example is themixed croppingofwinter fababean (Vicia faba L.) and winter wheat (Triticum aestivum L.). Adetailed description and thorough understanding of mixing effectswould allow plant production systems to better exploit thisphenomenon,forinstanceviabreeding.Willsuchyieldincrementsbefoundinthesemixedstands?Iftheyare,howcanthisinformationbeused toguideplantbreedingefficiently?Toaddress suchquestions,mixed stands ofN=8winter fababean lines a ndN=3winterwheatcultivarswerecomparedwiththeircorrespondingpurestandswithinthe framework of IMPAC³ (a German research project based at theUniversityofGoettingen).In2015,fieldexperimentsattwolocationsnearGoettingenwere implemented.Mixturesandpurestandswereconducted in a row intercropping design over 360 plots (10.5 m²each). Yield parameterswere observed in detail. Preliminary resultsshowsignificantyieldsurplusofmixedcropstandsoverpurestands.Moreover,thereisasignificantvariationofthismixingeffectcausedbythedifferentfababeanlines.

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P110–S13 AdvancementofwinterhardypeasandlentilsbreedinginHungary–Benefitsandpossibilities.Mendler-DrienyovszkiN.,DobránszkiJ.ResearchInstituteofNyíregyháza,UniversityofDebrecen,Hungary.Winter legumesareof greatecological andeconomical importance inthesustainableagriculture.Theirsowingoccursinautumnsotheplantscan be strengthened already in the same year before the winterthereforetheircultivationhaslessriskandresultinginhigheryieldthanthe spring planted legumes. Our breeding aim includes creating new,cold tolerant varieties that are suitable both for human consumption(winter hardy lentil) and animal feeding or even other special use(winter hardy pea). Beside cold tolerance the most important jointbreedingaimsincludedearlymaturation,plantheight,highandstabileyieldwithgoodquality;inthecaseofwinterhardylentilalsotheseedcolour. Two lines of generation F5 originating from new crossingsbelong in the ‘super-early’ maturity group. The winter hardy peabreeding lines have high plant height so the best practice may begrowing them in combination with cereals. One of the results of thewinterhardy lentilbreeding isavariety-candidatecalled ‘Pinklevi’andthree additional breeding lines with different seed colours. The‘Pinklevi’ is a cold tolerant variety-candidate belongs to the earlymaturitygroup.Themaincolourofthetestaofthedryseedispinkandochre. The size of the dry seed is medium, their weight is low.According to our previous studies and experiences these species aresuccessfullygrownalsoonacidicsandysoils.

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P111–S13 RoleofL-histidineinprotein-oilrelationshipinsoybeanseedDjordjevicV.,CeranM.,MiladinovicJ.,Balesevic-TubicS.,PetrovicK.,MiladinovZ.Instituteoffieldandvegetablecrops,NoviSad,SerbiaIn soybean, negative correlation between protein and oil is welldocumentedbutitispoorlyexplained.ThreeF2andF2:3populationswere developed, crossing between one high protein line and threehigh yielding genotypes. Based on protein content, from each F2population (approximately 150 lines per population) were selectedtenhighprotein (HP) and ten lowprotein (LP) lines and amino acidanalysisperformedonF2:3lines.Twoofthreepopulationshavehighnegative correlation between protein and oil content (-0.81 and -0.82),while in onepopulation this negative correlationwasweak (-0.42). Among all analyzed amino acids, only content of L- histidinefollow similar pattern. In populations with high negative protein-oilcorrelation, L- histidine was significantly lower in HP lines. On theotherhand,inpopulationwithweaknegativeprotein-oilcorrelation,content of L- histidine increased in HP lines. These findings canexplainweek negative correlation. Biosynthesis of L- histidine sharebiochemical reactions with oxidative phase of pentose phosphatepathway,whichproducesreducingequivalents(NADPH)necessaryforbiosynthesis of other compounds. Genotypes in this population canovercome this negative correlation by increased biosynthesis ofreducing equivalents, necessary for protein and oil synthesis. Thisfinding can be useful in further research, for breeding of betterqualitysoybeanandL-histidinecanbeusefulbiomarker.

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P112–S13 DiscoveringgeneticsignaturesofselectionintheelitesoybeangermplasmĆeranM.,ĐorđevićV.,MiladinovićJ.,Balešević-TubićS.,MikićA.,MikićS.,TrkuljaD.InstituteofFieldandVegetableCrops,MaksimaGorkog30,NoviSad,SerbiaIdentification of genomic regions affecting soybean improvementhaspracticalimportanceforfuturebreeding,indicatingpositionsofagronomically important genes or genes underlying adaptation.Genetic consequences of breeding in the environment of Central-East Europe were detected by analysis of ancestral and elitesoybeanvarieties,usinggenetichitchhikingmapping.Asaresultoflong-termselection,significantreductioningeneticdiversityoftheelite population comparing to ancestral was observed. Populationstructure of analyzed varieties has been largely influenced by thepedigree,causingalowlevelofgeneticdifferentiationbetweenthepopulations.Ninemicrosatellitemarkerswereconsideredasstrongpositive selection candidates, indicating regions involved in theimprovement and adaptation to target environment. In silicoanalysis detected 264 previously mapped quantitative trait loci(QTLs) located in the identified selectively important regions. Thelargest number had an influence on the reproductive period,followedbyseedcharacteristics,whole-plantperformanceandyieldQTLs.Beingthestrongestcandidatesforselection,markersSatt557and Satt357, located on Chromosome 6, revealed that E1 gene,which controls flowering time and time to maturity in soybean,seems to be amajor contributor for adaptation to environmentalconditionsofCentral-EastEurope.

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P113–S13 Constructionandevaluationofnear-IsogeniclinesforresistancetoAphanomyceseuteichesinpeaLavaudC.1*,LesnéA.1*,RiviereJ-P.1,ManginP.2,RoulletG.3,HerbommezJ.F.4,DeclerckP.5,FuretG.6,BoutetG.1,BarangerA.1,Pilet-NayelM-L.1*Contributedequallytothework1 - INRA-UMR1349 IGEPP, PISOM-UMT INRA/Terres InoviaBP35327, Le Rheu F-35653,France2-INRA,DomaineExpérimentald’Epoisses,BretenièresF-21110,France3-Unisigma,GIERechercheetSélection,FroissyF-60480,France4-KWS-Momont,MonsenPéveleF-59246,France5-RAGT2n,LouvillelaChenardF-28150,France6-LimagrainEurope,ChartainvilliersF-28130,FranceCommonrootrotofpea,duetoAphanomyceseuteiches,isoneofthemost damaging diseases in many countries. The development ofresistant varieties is a major objective to manage the disease.Consistent quantitative trait loci (QTL) controlling partial resistancewere discovered from four pea RIL populations (Hamon et al, 2013).This study aimed to validate the resistance QTL effects in differentgenetic backgrounds, by creating and evaluating Near-Isogenic Lines(NILs) carrying resistance alleles at individual or combined consistentQTL. Five then two parallel marker-assisted back-cross selectionschemes were developed, each consisting in transferring resistancealleles at one to three of the main QTL into three susceptible peacultivars.Atotalof157then36BC5orBC6NILs,carryingzerotothreeresistanceintrogressionswereobtained.TheNILswerephenotypedforresistancetoA.euteichesincontrolledconditionsatseedlingstage.Thefirst set of NILs was also phenotyped for resistance in multiple fieldenvironments. NILs carrying the major-effect QTL, individually or incombination with minor-effects QTL, had increased levels of partialresistance in both conditions. Several NILs carrying multiple minor-effectsQTLalsoshowedreducedlevelsofdiseaseseverityinthefield.ThisstudygivestoolsandinformationforthechoiceofresistanceQTLtouseinbreedingtoincreasepartialresistancetoA.euteichesinfuturevarieties.

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P114–S13 ComparisonofvernalizationresponseinwildCicerspeciesandcultivatedchickpeaKushwahA.,SinghS.DepartmentofPlantBreedingandGenetics,PunjabAgriculturalUniversity, Ludhiana,Punjab-141004,IndiaWildCicerspeciescancontributetoenhancethelevelsofresistancestomany stresses besides broadening the genetic base of cultivatedchickpea (Cicer arietinum L.). Vernalization insensitivity is a keyfeature of domesticated chickpea, and its genetic basis is not wellunderstood.Inthisstudy,theresponsetovernalizationwasstudiedincultivatedandwildCicerspeciesbelongingtoprimary,secondary,andtertiarygenepools.A totalof46germplasmaccessions including37wild and 9 cultivated germplasms were used in this study. All thegermplasmaccessionsweretestedwith(30daysat4°C)andwithoutvernalization (control). The difference in mean days to flowerbetweencontrolandvernalizationtreatmentswasusedtoassesstheflowering vernalization response. The difference in mean days togermination and mean days to first podding between control andvernalization treatmentswas also recorded to know the effect coldtreatment on days to germination and first podding. The wildgermplasm accessions are vernalization-sensitive, late floweringgenotypes while domesticated germplasm accessions arevernalizationinsensitive.Stronggenotypebyenvironmentinteractioneffect on days to flower was observed for all the germplasmaccessions.Chickpeabreedersinterestedinusingthewildprogenitoras a donor of exotic traits should be aware of the possibility ofintroducing vernalization response alleles that may alter thephenologyoftheirbreedingmaterials.

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P115–S13 ScreeningofdiverselentilgenotypesfortotalphenoliccontentGuptaS.,DikshitH.K.,PrakashP.,SinghA.,AskiM.ICAR-IndianAgriculturalResearchInstitute,NewDelhi,India.Phenolic compounds possess antioxidant activity and delay theoxidation of lipids, proteins, and DNA by inhibiting oxidizing chainreactions.Naturalphenolic antioxidants can scavenge reactiveoxygenandnitrogenspecies(RONS)therebypreventingtheonsetofoxidativediseases in the body. Eighty-five lentil genotypes comprising Indianreleased varieties/ advanced breeding lines and germplasm lines,Mediterranean landraces and ICARDA germplasm were evaluated fortotalphenolic content (TPC).Spectrophotometric technique,basedonFolin-Ciocalteau reagent was employed and calculated as Gallic AcidEquivalents per gram of extract. The total phenolic content (TPC) oflentilgenotypesvariedfrom0.756(L-11-231)to3.043(IC262839,L-11-295) mg gallic acid equivalents (GAE)/g sample. This study will helpbreeders not only to develop lentil lines with better phyto-nutrientprofile but can also be promising in phytomedicinal andpharmacologicalformulations.

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P116–S13 Breedingforintercropping:joinappliedgeneticsandagronomyforimprovedannuallegumeproductionMikićA.1,DucG.2,BedoussacL.3,ĆeranM.1,Corre-HellouG.4,DeRonA.M.5,ĐorđevićV.1,FloriotM.6,JeuffroyM.-H.7,JustesE.3,LecomteC.2,MihailovićV.1,MikićS.1,ŠarūnaitėL.8,VasiljevićS.11-InstituteofFieldandVegetableCrops(IFVCNS),NoviSad,Serbia2 - InstitutNationalde laRechercheAgronomique(INRA),UMR1347Agroécologie,Dijon,France3-InstitutNationaldelaRechercheAgronomique(INRA),UMR1248AGIR,Castanet-Tolosan,France4-GroupeESA,Angers,France5-ConsejoSuperiordeInvestigacionesCientíficas(CSIC),MisiónBiológicadeGalicia(MBG),Pontevedra,Spain6-Agri-Obtentions,Paris,France7 - Institut National de la Recherche Agronomique (INRA), UMR 0211 Agronomie,Thiverval-Grignon,France8-LithuanianResearchCentreforAgricultureandForestry, InstituteofAgriculture,Akademija,LithuaniaArchaeology offers evidence that growing plants together, withannuallegumesasusuallyinevitablecomponent,couldbethemostancientcroppingsysteminallprimevalagriculturalcentres.Withatenmillennia long tradition, intercropping annual legumes, usuallywithcerealsand fordiverseuses,has remained importantallovertheworlduntiltoday.There isaphenomenonthatbringstogetherlegume breeders and agronomists: both are aware that there aredifferencesintheagronomicperformanceofthemixturesofannuallegumesandotherfieldcropsifdiverseannuallegumecultivarsareused.Wishingtounderstandthisphenomenonproperlyanddefineits economic significance, we are establishing a firm interactionbetweenbreedersandagronomists, inordertodesignsuchannuallegumeideotypesthatwouldhavethebestagronomicperformancewhenintercroppedwithdiverseplantsforeitherforageorgrainorbiomass or any other use. Our major hypotheses are that theideotypes for intercropping are the genotypes being the mostcompetitiveinthesameenvironmentcompartmentsortakingprofitof the complementary compartments.Howeverdemanding, this isfeasible: thepreliminary schemes for forageandgrainproduction,developedmostlyat IFVCNS,producedencouraging results in fieldconditions.Jointeffortsofannuallegumebreedersandagronomistsshould be beneficial for both scientific community and result inenhancingthelegumecultivationandproductioningeneral.

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P117–S13

Theproteinquantityandqualityinbreedingofsoybean,chickpeaandbeantocreatecultivarsAbugaliyevaA.I.,DidorencoS.V.LLP"KazakhResearchInstituteofAgricultureandPlantGrowing",KazakhstanThemainlegumeseedsbiochemicalcomponentisaprotein.Objective:Toevaluatethepotentialofsoybean,chickpeaandbeancultivarsforproteincontentandstabilityofitsformation.InKazakhstan registry thereare30 soybeanvarieties, including10fromKazakhstanbreeding,representedwith5maturitygroupsfromtheearly"00"tolate-maturing"III".Ontheproteincontentstability,Misula-leadingvarietyintermsofstate variety test 40,1 ± 0,7% and the Radost 1-39,8 ± 0,5%. Theshare of soybean genotypes with a protein content above 40% ismaximummarked for varieties Perizat, Paradis, Khorol andGallek,also for Atlanta, Renta, Ruzhica, Riza, Selecta 302, Cheremosh,Selecta201,Iskra.Soybean cultivars yield in a demonstration (domestic and foreignbreeding)nurseryvariesdependingonthegenotype,growingyearandmaturinggroupfrom1.0t/hato6.2t/ha.Theproteincontentin soybean seeds as awhole ranged from 32.2% (LADA) to 46.9%(Horol).Maximum"proteincontent"observedfor00and0maturinggroup.Germplasm soybean set genotypes (Kazakhstan, Russia, Serbia,Ukraine, Moldova and the United States) are characterized byprotein from21.0 to 50.0% (Smena cvs andKaryagin Pamyat cvs).Chickpeas breeding nurseries on the protein content varied from26,6%to31,8%,beanfrom21,5%to26,9%.A number of samples selected on the high Fe content: (Vavilovcollection, KRIAPG, and foreign)methionine, cysteine content andbalance11S/7Sglobuline.

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P118–S13 NewportuguesechickpeavarietiesDuarteI.,PereiraG.,BarcelosC.,MenesesM.INIAV,Elvas,Apartado6,7350-951Elvas.PortugalAn increase of grain legume production is essential formeeting foodproteinneedinEurope.PortugalislocatedintheWestpartofIberianPeninsula(latitude37º-42º N and longitude 9º - 6º W); this southerly latitude gives it aMediterraneantypeofclimate,characterizedbyhotanddrysummersandcoolandwetwinterssohassuitableconditionstoproducepulsescrops,suchaschickpea(CicerarietinumL.).Chickpea breeding is being conducted at INIAV-Elvas, Portugal since1986. Five varieties have been released: 3 kabuli type (‘Elvar’,‘Eldorado’and‘Elixir’)and2desitype(‘Elmo’and‘Elite’). ‘Elvar’ isthemostcultivatedvarietyinPortugalandsouthFrance.Selection criteria include autumn/winter growth adaptation, hightolerance toAscochyta rabiei andFusariumoxysporum,adaptation toabioticstresses(hightemperaturesandwaterlogging)andseedsize.From 100 advanced lines, included on adaptation trials in the last 4years,wereselected3genotypes:1desiand2kabuli.Those genotypes show good adaptation to different environmentalconditionswithhighyieldandtolerancetodiseases.These3advancedlineswillberegisteredonthePortugueseCatalogueofVarieties.

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P119–S13 ApplicationofNIRStechnologytogeneticallyanalysevicineandconvicinecontentinfababean(ViciafabaL.)Puspitasari,W.,LinkW.DepartmentofCropSciences,Georg-August-UniversityofGoettingenFababean,beingagrainlegume,providesseedproteinandstarchforhuman and animal nutrition. However, faba bean seeds containantinutritivecompoundssuchasvicineandconvicinethatnegativelyaffectdigestibility.Moreover, vicine and convicine are the causativeagents of favism (haemolysis) in such humans who are geneticallydeficient inglucose-6-phosphate-dehydrogenase.Thepurposeofthecurrentexperiments is todevelopavalidnear infraredspectroscopy(NIR) calibration for the quantitatively varying vicine and convicinecontent of such faba beans which do not have the low-vicine gene“vc—“; and to study their genetic variation of these compounds.Spectra and HPLC-results of milled seed of 147 winter faba beaninbredlinesand75furtherfababeanlineswereusedtodevelopandvalidate the calibration. The currently available NIRS calibrationequationisabletoreasonablypredictvicineandconvicinecontentinfaba bean with a standard error of calibration SEC = 0.07 andcoefficientofdeterminationRSQ=0.91.Analysesareongoing,yetwealreadybynowseealargequantitative-geneticvariationofvicineandconvicinecontent.

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P120–S13 MethodsforthedevelopmentnewpealinesDostalovaR.,HunadyI.,LudvikovaM.,OndrackovaE.AgritecPlantResearchLtd.,Sumperk,CzechRepublicTheaimofthisworkwastoincreasegeneticdiversityoffieldpea.Forthecreationofnew linesofpeaswereuseduniquegenetic resourceswithaccumulatedresistancegenesfromthegenebankofAGRITEC.69newpeaaccessionswereobtainedfromgenebankVIRStPetersburg.Accessions were evaluated under field conditions, morphologicallydescribedusingDescriptor listofgenumPisumL.andtheincidenceofdiseasesandpestinfestationswasevaluated.Declared sources of resistance were tested in inoculation tests.Molecular analyses were performed to speed the breeding process.eIF4E (iso) corresponding loci SBM-1 (LG VI) and SBM-2 (LG II),mediating recessive resistance toseveralviralpathogensof thegenuspotyvirus(includingPSbMV)wereidentified.AllelesofeIF4Egenewereidentified in selected genotypes of pea, involving all PSbMV donorsused in practice. Based on sequence analysis PCRmolecularmarkers,allowingthe100%reliable identificationofboththehomozygousandheterozygousplantsweredesigned,testedandselected.GeneticdiversityofpeaaccessionswasdeterminedusingRBIPmarkers.Molecular detection of resistance to powdery mildew, Fusarium wiltand PEMV were carried out. Molecular detection of trypsin inhibitoractivity was performed and results were compared with chemicalanalysis.Suitablematerialswithhighlevelsofbiologicalpropertieswereusedinthehybridizationprocess,obtainedlinesareusingforbreeding.

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P121–S13 QTLdetectionofpod-relatedtraitsinPea(Pisum

sativumL.).GuindonM.F.,MartinE.A.,CraveroV.P.,CointryE.L.IICAR-CONICET(InstitutodeInvestigacionesenCienciasAgrariasdeRosario).Pea is a self-pollinated, diploid (2n=14), annual crop producedworldwide forhumanconsumptionandanimal feed.Peabreedinghas been conducted with the aim of developing high yieldingcultivars. QTL detection will provide additional tools to identifydesirableallelesearlyinthebreedingprocess,reducingthetimeofcultivarrelease.ThepurposeofthisworkwastodetectandvalidateQTLsrelatedtonumber(NP),length(LP)anddiameter(DP)ofpodsin pea. A total of 50 SRAPmarker combinations and 10 SSRwereevaluated in 112 F2 individuals derived from a cross betweenDDR14 and Explorer commercial lines. The phenotypic traits weremeasured in the F2 population in 2013 and in their F3 families in2014and2015.ThenormalityofeachtraitwasevaluatedusingtheShapiro-Wilk´stest.Themendeliansegregationofeachpolymorphicmolecularmarker was verifiedwith a χ2-test. Associations amongmendelianmarkersandtraitsweredetectedbyANOVAsinglepointanalysis.ConsistencyoftheassociationsfoundinF2individualsandF3 families were evaluated to validate the QTLs. All themorphological traits presented normal distributions and 285molecular markers showed mendelian segregation. The analysisallowedthevalidationof11QTLs:3markersassociatedtoNP(5to10%ofphenotypicvariationexplained),4markers related toLP (3to 17%) and 6 markers related to pod diameter (4 to 11%). Thiswork provides interesting information for use in pea breedingprograms.

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P122–S13 GeneticassessmentofmutantpopulationsofLentilandChickpeaLaouarM.1,TabtiD.1,TiliouineW2.,AbdelguerfiA.21 - E.N.S.A Laboratoire d’Amélioration Intégrative des Productions Végétales, El-Harrach,Alger,Algérie2 - E.N.S.A Laboratoire des Ressources Génétiques et Biotechnologies, El-Harrach,Alger,Algérie3 - Division of Biotechnology and plant breeding, National Institute of AgriculturalResearchofAlgeria(I.N.R.A.A.),Baraki,Algiers,16000,Algeria.tiliwahiba@yahoo.frLentil (Lens culinaris Medik.) and Chickpea (Cicer arietinum L.) areimportant foods legumes crops with high nutritional value andecological importance.Algeria isthethird(chickpea)andfifth(lentil)largest importer in theworld.Theproductionsarevery lowbecausespecially the lack of adapted and productive varieties. Radio-mutagenesis is one of ways to create new variability for selectionaccordingtoenvironmentalconditions.Manyusefulgeneticchangeswereinducedbyamutagenictreatment.Gammarayswerethemostusedmutagen to change gene(s) in lentil and chickpea due to theireasy application. Mutation was induced using gamma rays in twovarieties Idlib-3 (lentil) and Ghab 4 (chickpea) by 100 and 258 Gycorresponding to the lethal doses (DL50) respectively. For eachspecies, from10000 seedsM1wegeneratedabout140 familiesM1andabout4000genotypeswerecharacterizedinthefield.Yield,yieldcomponent and harbor variability, heritability, and genetic advancewereassessedonputativemutant. The rangeof variability formaintraits was high. High correlation showed between number of totalpods and seed yield. About 3% of families’ mutants (M2) showedsuperiority than the control for days to flowering, days tomaturityand other traits. High heritability associated with high geneticadvancewasobservedfromseedyieldandhundredseedweight;thisindicates theexpectedeffectivenessofselection for the traitsunderconsideration.

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P123-S13 AdaptationofPeatocontrastedFrenchregions:simulationofpeavarietieswiththeAZODYN-PeacropBénézitM1,LarmureA.2,Munier-JolainN.2,JeuffroyM.-H.11 - UMR Agronomie INRA, AgroParisTech, Université Paris Saclay, 78850 Thiverval-Grignon,France2-UMRagroécologie,DijonPea(PisumsativumL.)isaparticularlysensitivecropregardingabioticstress throughout its cycle (Schneider and Huyghe 2015). Climatechange results in the increase of the unpredictability of both thefrequencyandtheintensityofthesestresses(Stockeretal.2013).Toavoid abiotic stress, breeders have been developing, for the lastfifteen years, winter pea varieties sown during autumn, moreresistant to frost with an earlier flowering date than spring pea, aswellasvarietiescalled“Hr”,photoperiod-sensitive,thatcanbesownevenearlierthanregularwintertypes.However,despitetheprogressof winter type breeding, winter pea surfaces seldom reachedmorethan25000hasince2000.Ouraimwastostudytheperformancesofthe different types of pea in contrasted French regions, for variousclimates, in order to adapt the choice of the pea type to thefrequency and intensity of abiotic stress. For this purpose, the cropmodel Azodyn-Pea (Jeuffroy et al. 2012) was adapted to simulatevarioustypesofpea.ThemodelwasthenusedfortheevaluationofthethreetypesoverthepasttenyearsindifferentFrenchregionsinorder to identify which type is most suited to each environment(climate, region). Here we show the potential of winter pea forvarious French climates. Azodyn-Pea will also allow, in a secondphase, theexplorationofnovelcombinationsofplant traits inorderto design ideotypes and contribute to the development of newvarietieswithineachtype.

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P124–S13Cost/BenefitAnalysisofMarkerAssistedSelectioninaFieldPeaBreedingProgram.RosewarneG.M.,SlaterT.,PandeyB.,KaurS.BiosciencesResearch,AgricultureVictoria,Australia.Many molecular markers are currently available in field peas andthesearebeingappliedintheAustralianfieldpea-breedingprogram.As the breeding program operates on a fixed budget, theimplementation of markers requires both genetic and economicanalyses todevelop costeffectivemodels that give thebest geneticoutcomes. This paper compares costs of using phenotypic selectionforarangeoftraitsandcomparesthosecostsandtheoreticalgeneticoutcomesagainstdeployingmolecularmarkerstopyramidthesamesetoftraits.Efficient, controlled environment assays have been developed toscreen for tolerance to salinity and boron, and for resistance todownymildew (2pathovars),Pseudomonas syringae pvpisi (race3)andP.syringaepvsyringae.Thesetraitsaresimplyinheritedwithoneto two loci conferring each of the desired phenotypes. Costs for allassays(controlledenvironmentandmolecular)havebeencalculatedbasedonconsumablesandstaff time.Phenotypic selection involvedapplying different assays to large segregating populations inconsecutivegenerations,(F2-F5)withallphenotypesconfirmedonF5families.Marker assisted selection applied allmarkers to enrich forappropriatealleles in theF2generation,andconfirmedhomozygousstatus intheF5generation.Theapplicationofmarkerswas1/6thofthe cost of phenotypic selection and also gave better geneticoutcomes with regard to the frequency of germplasm thatrecombinedalltraits.

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P125–S13 GenotypexenvironnementinteractioninwinterpeaBiarnèsV.,PontetC.TerresInovia,Grignon,FranceAnanalysisofgenotypexenvironnementinteractionhasbeenmadeonthe winter pea trials network of registered varieties, coordinated byTerres Inovia in 2015. 10 winter pea varieties, recently registrered,weresownin25sitesindifferentfrenchareas.Themainresultsare:- Genotype x environnement interaction is about 4,3 % of the totalvariationobserved foryields. It ishigher thanvarietyeffectbutmuchsmallerthantheenvironmenteffect,whichexplainsmorethan80%ofthetotalvariation.-Groupsofvarietieswithsimilarbehaviourandgroupsofenvironemntswithsimilarranksofvarietieshavebeenfound.- Variables calculated from development stages and climate allow toexplaingenotypexenvironementinteraction.

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P126–S13 ShortdurationpigeonpeahybridstobridgedecadesoldyieldHinganeA.J.1,RathoreA.1,KumarC.V.S.1,ChandU.1,SinghS.J.21 - International Crops Research Institute for Semi-Arid Tropics, Patancheru, India-5023242 - Sri Karan Narendra Agriculture University (SKNAU), Rajasthan AgricultureResearchInstitute(RARI),Durgapura,Jaipur,302018,IndiaLowyieldinpigeonpeahasalwaysbeenamatterofconcernforallthe stakeholders over more than last five decades. But,development of CMS based hybrid technology in pigeonpea, hasproved the potential to bridge this yield gap. Several mediumdurationhybridshavebeenreleased instatesof India,yielding25-40%higher yield than local varieties, but these hybrids are regionspecificduetoitsphoto–thermosensitivenature.Hence,needwasfelt toexploreotherpossibilities to increasepigeonpeaproductionandproductivity.Recentlydevelopedphoto-thermoincentiveshortduration varieties have helped to identify new production nicheslike wheat and rice cropping system in non-traditional states ofIndia.Butoveryears,productivityof theseshortdurationvarietieshas also become stagnant. Considering this, a hybrid-breedingprogramwas initiatedat ICRISATtodevelop locationspecific shortduration hybrids. Nine short duration experimental hybrids alongwith two checks were evaluated for its yield and adaptability atICRISAT,PatancheruandRARI,DurgapuraRajasthanforthreeyears(2013-15) using GGE biplot analysis. Among the six environmentsevaluated,Patancheru(Pat-15)wasidentifiedassuitablelocationindiscriminating short duration pigeonpea hybrids and can beconsidered as ideal testing location. ICPH 2364 followed by ICPH3310wereearliesttomaturewhileICPH2429andICPH2433werefoundtobehighyieldersandstableacrossboththelocations.

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Session 14, plenary: Frontiers in legume agronomy -RoomArrábidaI&IIChairedbyErikS.Jensen(SLU,Sweden)andSusanaAraújo(ITQBNOVA,Portugal)10:30-11:00 Key lecture - Eric Justes: Synthesis on theeffects of grain legume insertion and cereal-grain legumeintercropsinlowinputcroppingsystemsinSouthernFrance

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OralCommunications11:00-11:15Oral–S14Designandassessmentoflegume-basedcroppingsystemswithstakeholdersinEuropePelzerE.1,BourletC.1,CarlssonG.2,Lopez-BellidoR.J.3,JeuffroyM.-H.11 - UMR Agronomie INRA, AgroParisTech, Université Paris Saclay, 78850 Thiverval-Grignon,France2-SLU,DepartmentofBiosystemsandTechnology,POBox103,SE-23053ALNARP3- University of Cordoba, Edificio C4 "CelestinoMutis", Ctra.Madrid-Cadiz km 396,14071Cordoba,SpainLegume-based cropping systems have numerous environmentalbenefits, but stakeholders are often not aware of all of them.Moreover, there is a large range of available species that can begrown with various practices in the fields (sole or cover crop,intercrop,etc.).Our aimwas thus to design legume-based locally adapted croppingsystems, in threeEuropean regions,valuing thediversityof legumesspeciesandpractices.Afterdescribing themost frequentcropping systems ineach region,local improvement targets were defined. Then, during a designworkshop gathering scientists and a few advisors and farmers,knowledge on legume crops were shared, and then innovativecroppingsystemsweredesigned.With the aim of assessing them with the multi-criteria tool ®Masc(toolaccountingforuser’spreferencesonsustainability),wesurveyedstakeholders in each country (29) to gather their preferences onranking of performance criteria (weight sets) and to collect theiropinion on the feasibility of designed cropping systems. We thensynthesizedtheseweightsetsinto4MASCtreesandeachinnovativecroppingsystemwasassessedaccordingtothosetrees.In the end, for each region, we identified legume-based croppingsystems,whichwereassessedsustainablebythe4stakeholdertreesand said feasible. The whole study also allowed identifying lack ofknowledgeon legumecropsandtheireffectsonthe followingcropsthatshouldbecompletedforpromotinglegumesinEurope.

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11:15-11:30 Oral–S14Performanceoflegume-basedannualforagecropsinthreeMediterraneanregionsPorquedduC.1,MelisR.A.M.1,Thami-AlamiI.2,AbbasK.3,PecettiL.4,AnnicchiaricoP.41 - Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo (CNR-ISPAAM),Sassari,Italy2-INRA-CentreRégionaldeRabat,Rabat,Morocco3-INRAA-Agro-systemEastDivision,Sétif,Algeria4 -CentrodiRicercaper leProduzioniForaggereeLattiero-Casearie (CREA-FLC),Lodi,ItalyLegume-based forage crops may be pivotal for improving thesustainabilityofMediterraneancrop-livestocksystems.Theobjectiveof this study was identifying key species and optimal cropcomposition across three drought-proneMediterranean regions, onthebasisofcropperformanceandfarmers’acceptability.Cultivarsofthree legume species (field pea, semi-dwarf and tall types; Narbonvetch;commonvetch)andtwocereals (oat; triticale)weregrown inSassari(Italy),Sétif(Algeria)andMarchouch(Morocco)aspurestandsand legume-cereal binary and four-component mixtures, assessingcropdry-matteryield(DMY),legumeproportionandweeddryweightovertwocroppingyearsinfour-replicationexperiments.Largefarmergroups assigned acceptability scores (1=poor, 5=excellent) beforeharvesting.Onaverage,binaryandcomplexmixturesshowedhigherDMY than pure stands. Both pea types in pure stand or inmixturewithoat,commonvetchinmixturewithoatandthecomplexmixtureofvetchandcerealspecies,tendedtocombinehighforageyield,highfarmeracceptabilityscore(aroundorover4),andhighweedscontrol.Pea-based binary mixtures had higher DMY than vetch-based ones(6.0 vs 5.2 t ha-1). Themean legume proportion of binarymixtureswas53%forpea,45%forcommonvetch,and31%forNarbonvetch.Ourresultspointtotheparticularinterestofpeacrops(inpurestandor in mixture) as an alternative to vetches in drought-proneMediterraneanenvironment.

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11:30-11:45 ORAL–S14Legume-basedmixedcroppingsystemsmayhavehigherwateruseefficiencythanmonocropsystemsLingnerA.1,2,DittertK.1,SenbayramM.31-InstituteofAppliedPlantNutrition,UniversityofGöttingen,Germany,2-CentreofBiodiversityandsustainableLandUse,UniversityofGöttingen,Germany,3-InstituteforPlantNutritionandSoilScience,HarranUniversityofSanliurfa,TurkeyImproving sustainability and productivity of agricultural systems is amajor concern facing global change. The increasing probability ofseasonaldroughtsand freshwaterscarcityemphasizes the importanceofcroptraitssuchaswateruseefficiency(WUE).Inthiscontext,multi-speciescropstandswithlegumesarelessdependentonexternalinputsandmayhavehigherresourceuseefficiency.Our study aimed to evaluate twodifferent agro-ecosystems: croplandand grassland, cultivated as mono or mixed cropping with legumes(faba bean and white clover). Canopy WUE in each treatment wasdetermined via transparent chambers connected to a gas exchangesystem (GFS-3000, Heinz Waltz GmbH, Germany). Additionally, aQuadrocopter (Raptor, EagleLive Systems GmbH, Germany) equippedwithaspectralcamera(ADCMicro,TetracamInc.,California)wasusedtomeasurenormalizeddifferencevegetationindices(NDVI).The gas exchange data clearly showed that in cropland both net-photosynthesisandWUEwerehighest inmixedcropping(fababean+wheat) in summer 2015,while during a temporal drought period theevapotranspiration was lowest. In pure white clover stands, net-photosynthesiswasconsiderablyhigherthanbothinryegrassandtheirmixed stands.NDVI valueswere almost similarwhen comparing purelegumestandsandtheirmixedcropping,whichwassignificantlyhigherthan pure wheat or grassland stands. Both methods suggested thatmixedcroppingsystemsimprovedproductivityandWUE.

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11:45-12:00 Oral–S14Participatorydevelopmentofgrainlegume-cerealintercropsforenhancedproductivityandreducedweedabundanceinorganiccropproduction.CarlssonG.1,ModigP.2,HunterE.3,JensenE.S.1.1-SwedishUniversityofAgriculturalSciences(SLU),DepartmentofBiosystemsandTechnology,Alnarp,Sweden.2-SwedishRuralEconomyandAgriculturalSocietyinSkåne,Kristianstad,Sweden.3 - SLU, Department of Work Science, Economy and Environmental Psychology,Alnarp,Sweden.Grain legumes such as beans, lentils, lupins and peas are highlyappreciated for their symbiotic nitrogen fixation and for providinghigh-qualityfoodandfeed,butperceptionsofloworvariableyieldsmay limit farmers’ interest to grow grain legumes. In addition,despitedocumentedbenefitsofcropdiversificationintermsofyieldstability and resource use efficiency, European agriculture is stillmainly based on sole crops. We present results from an ongoingprojecttogetherwithorganicfarmersinsouthernSweden,designedto evaluate the empirical generalizability of production benefits inintercropping systems.Thework startedbyagreeingonobjectivesconcerning evenness in crop maturity, low weed abundance andhigh crop quality, that need to be fulfilled in order for theparticipatingfarmerstogrowmoreintercrops.Grainlegume-cerealcombinations that appear promising according to farmers’experience and scientific results were then identified, and theirperformance tested in on-farm trials and research-station fieldexperiments. The results obtained so far show clear reductions inweed abundance with increasing proportion of the cerealcomponentinlentil-oatandlupin-wheatintercrops,andhighertotalyields in intercrops than insolecrops.Theresultsandexperiencesfromtheprojectwillbediscussed in thecontextofpromoting theimplementationofintercroppingandenhancingthesustainabilityofSwedishorganicgrainlegumeproduction.

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PostersP127–S14 Theeffectsoflegumecrops(peaandfababean)onyieldandqualityparametersoffollowingcabbagecropsunderorganicproductionconditionsBalliuA.1,LikoJ.2,RadaZ.1,SallakuG.11-AgriculturalUniversityofTirana,Albania2-TechnologyTransferCenterofLushnja,AlbaniaFortydayoldbroccoliandcauliflowerseedlingsweretransplantedinthree equal field blocks previously cultivated with respectively;wheat, field pea and faba bean. The common conventionalproduction technology, including the use of chemical fertilizers wasapplied inwheat, but no fertilizers at allwereused in fababeanorfield pea blocks. Field transplanting of broccoli and cauliflowerseedlings was conducted according to a three replicationsrandomizedblockdesignatanequalplantingdensity(0.6mx0.3m)and common organic production practiceswere applied throughoutplant’ growing cycle till the end of November 2015. A significantlyhigher abovegroundbiomasswas recorded in the cauliflower cropsfollowed faba bean, versus both field pea and wheat, but nodifferencewasfoundregardingbroccoli.Aswell,significantlyhigherNtotalcontentandN-NO3contentwasfoundintheleavesofbroccoliand cauliflower crops followed faba bean compared with therespective crops followed either field pea or wheat. A significantlyhigher percentage of curd formation, as also the highest curd yieldeither in broccoli or cauliflower were harvested from the cropsfollowed faba bean and then field pea. While a significantly higheraverage curd weight was found in cauliflower crops following fababean, but not in broccoli, yet no differences were found regardingtotalN,PandK content inbroccolior cauliflower’s curddue toprecropplant.

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P128–S14 Suboptimalenvironmenttemperaturesaffectgrowthandthemorphologyofrootsysteminpea(Pisum

sativumL)plantsSallakuG.,BalliuA.AgriculturalUniversityofTirana,AlbaniaSeeds of three different pea genotype were sown in styrofoamtransplant trays filled with vermiculite and latter placed in twodifferent growth chambers, respectively at air temperaturesof 10°Cand15°C. Inbothchamberstherelativehumiditywasmaintainedat90%,PPFD180µmolm2s-1andthephotoperiod12h.AtDAS7,9and12, ten plant of each treatment were selected randomly, dissectedand separated into roots and shoots. The root systemwas scannedandanalyzedandsubsequently,allplantorgansweredried(65°C,48h) and weighted separately. The environment temperature hasaffected thedrymatter accumulationofnewlyemergedpeaplants.Low temperature has significantly reduced the overall plant drymatter, but it has favored a higher proportion of root dry matterversus the totalplantmatter.However,depend lessofenvironmenttemperatures, root to whole plant ratio was gradually decreasedalongside plant growth. The morphology of root system was alsohighlyinfluencedbytheenvironmenttemperature.Undersuboptimalenvironmental temperatures, the root system of pea plants is lessbranched,i.e.,thenumberoftipsandforkswassignificantlyreduced.Total root length, root projected area, root surface area and rootvolume were as well significantly smaller, while the average rootdiameter was strongly increased. Significant differences regardingroot morphology parameters did also found among differentgenotypes.

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P129–S14 FoliarapplicationoffreeaminoacidsimprovedalfalfaperformanceunderrainfedconditionsPooryousefM.1andAlizadehK.21-DepartmentofAgronomy,Mahabadbranch,IslamicAzadUniversity,Mahabad,Iran2-DrylandAgriculturalResearchInstitute(DARI),Maragheh,IranThisworkinvestigatedtheefficiencyoffreeaminoacidcompoundstoimprove production of alfalfa (Medicago sativa L.) under rain-fedconditionsduring2011-2012.Experimentwasconductedascompleterandomized blocks design with three replications at three alreadyestablished alfalfa cv. Garayonja fields. There was two differentsprayingtimesincludingonesprayatearly4-6leavesstageandthreetimes spraying startedat 4-6 leaves stagewith twoweeks intervals.Theexperimentincluded12treatmentsresultedfromfactorialofsixspraytreatmentsandtwosprayingtimes.Spraytreatments includedAminol-forte, Kadostim, Fosnutren and humiforte at 1 L/ha, alongwith water spray and no spray as control. Results indicated thatincreasing the spraying times of plantswith amino acid compoundssignificantly (P<0.01) increasedplantheight,drybiomassweightandcrude protein content compared to control. Different amino acidcompoundsshoweddifferenteffectsregardingthestudiedtraits.Thehighest biomass achieved in using Kadestim and application ofAminol-forteh resulted to the highest crude protein content. It wasconcluded that foliar spray of free amino acids could enhance thequalityandquantityofalfalfaintherain-fedconditions.

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P130–S14 EvaluationofdifferentorganicallycultivatedgrainlegumesinNorthernGermanyBöhmH.,AulrichK.Thünen-InstituteofOrganicFarmingField trials at the Thünen-Institute of Organic Farming in NorthernGermanywere conducted in the years 2011 to 2015 in a randomizedblockdesignwithfourreplicates.DifferentgrainlegumessuchasViciafaba (field bean, cv Divine),Pisum sativum (pea, cv Respect), Lupinusangustifolius (blue lupin, cv Boruta) and L. albus (white lupin, cvFeodora) were compared to two varieties of Vicia sativa (commonvetch,cvInaandSlovena)aspurestandandintercroppedwithoat(cvGalaxy)andthreedifferentseedratios(75%:25%,50%:50%and25%vetch:75%oatoftherespectiveseedrateinpurestand).Intheyears2014 and 2015 peas, field beans and blue lupins were cultivatedintercroppedwith100%oftheseedrateofgrain legumesand25%oftheseedrateofoat.White lupin showed thehighest yield, crudeprotein (CP)-content andCP-yield, but the cultivation is not recommendable due to the highsusceptibility toanthracnose.Yieldof fieldbean is lowercomparedtowhite lupin but higher compared to the pea and blue lupin. Thecultivation of vetch-oat intercrop is necessary due to the low lodgingresistance. The seed ratio of 50% vetch and 50% oat had a higherlodging resistance and a better weed suppression compared to theintercropped vetchwith 75% vetch in the seed rate. Yield of Slovenawas higher compared to Ina. CP content of vetch (33.4%) wascomparabletothatofbluelupin(33.6%)andhigherthaninfieldbean(29.0%).PeashadthelowestlevelofCP(19.6%).

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P131–S14 Effectofsulfurfertilizationandvarietyonyieldandcontentsofcrudeproteinandaminoacidsoforganicallyproducedbluelupins(LupinusangustifoliusL.)WittenS.,AulrichK.,BöhmH.Thünen InstituteofOrganic Farming - FederalResearch Institute forRuralAreas,ForestryandFisheriesGrain legumes, like blue lupins, are important protein feedstuffsespecially inorganic farming.Their contentofcrudeprotein (CP)and essential amino acids (EAA) is of great interest. The sulfur-containing amino acids (SAA)methionine and cysteine are oftenlimiting in common diets primarily for poultry. Therefore, weinvestigated, whether sulfur fertilization of blue lupins couldenhancetheircontentsofSAAandifithasothereffectsregardingcropyield,CPandfurtherEAA.Twobranched(Boregine,Probor)andtwodeterminated (Boruta,Sonet) blue lupin varieties were cultivated at an experimentalstation inNorthernGermany in the years2012, 2013, and2015.ThevarietieswereeithergrownwithorwithoutSfertilization(40kgSha-1;MgSO4).SampleswereanalyzedforCPandaminoacids(AA)withNIRS.StatisticalanalysesweredonewithSAS9.4 (procglm).The yield and the contents of CP and AA differed depending onvariety in theobservedyears.Boregineshowedthehighestyieldand Probor the highest CP content. Boruta had the lowest yieldand CP content. Sulfur fertilization was able to increase theamount of SAA in some varieties but tended to decrease lysineandthreoninecontentwithoutaffectingcropyieldorCPcontent.This might be due to an altered storage protein ratio. Hence,sulfur fertilization could be beneficial by increasing SAA in bluelupin varieties. Further investigations on the effect of sulfurfertilizationforotherlegumesarerequested.

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P132–S14 Beans:fromseedtoseedSousaM.1,FernandesH.A.2,RibeiroC.J31 - Frescura Sublime Unipessoal Lda., Rua Cruz do Romeu, 510. Ronfe, 4805-406Guimarães.sousamaf@gmail.com2 - Centre for the Research and Technology of Agro-Environmental and BiologicalSciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Quinta dePrados,5001-801VilaReal,Portugal.hortenseaf@hotmail.com3 - University of Trás-os-Montes and Alto Douro (UTAD), Department of Agronomy,QuintadePrados,5001-801VilaReal,Portugal.cribeiro@utad.ptLeguminousspeciesarepartofthePortuguesegastronomictraditionand Mediterranean diet, but in recent decades their consumptionsufferedasharpdecline.So,itisveryimportanttheimprovementoftheir consumption by using different stages of plant development,basedonabestknowledgeoftheculturalpracticesanditsrelationtothequalityofpodsandseeds.The use of ancient cultivars and good agriculture practices areimportantwaystopreservethegeneticresourcesandmakinguseofspecies and cultivars already used and adapted to our agriculturalconditionsandusedbyourancestors.Bythisway, itwillbepossibleto identify thebeststagesofpodandseedmaturity forharvestandestablish the time elapsed between sowing and the desiredmaturationstage.The offer of new products with high degree of quality requires themonitoringofallculturalpracticesandtherecordingofallphasesofproduction. The main objective of this work is to present thesequence of cultural practices between sowing and harvest atdifferentdevelopmentstagesofpodsandseedsfromcowpea(Vignaunguiculata) andbean (Phaseolus vulgaris). Also, itwill be recordedthevegetativeandthereproductivephaseofeachspecies,presentingtheculturalcyclefortheseplantsproducedattheNorthofPortugal.The main use of the production is a freezing process of immaturefruitsandseedsinsteadofthecommonuseasdriedseed.

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P133–S14 EfficacyofdifferentherbicidetreatmentsinatriallocatedinMontijo(Extremadura,Spain)Gragera-FacundoJ.,Lozano-RuizM.J.,Maya-BlancoV.,González-LópezF.CICYTEX,JuntadeExtremadura,SpainThe effect of four herbicide treatments has been tested in sixdifferent legume species in a trial located in a commercial farmlocated atMontijo. The applied treatments were: 1 (pendimetaline33%,3,5L/ha+linuron45%1L/ha,preemergence),2(pendimetaline33%,3,5L/ha+linuron45%1L/ha+imazamox4%3L/ha+aclonifen60%2,5L/ha,preemergence),3(imazamox1,67%pluspendimetaline25%3L/ha+clomazone36%0,25L/ha+aclonifen60%2,5L/ha)and4 (fluazifop-butil 12,5% 1,5 L/ha, posteemergence). Legume specieswere white lupin, chickpea, pea, field bean, vetch andVicia ervilia.Herbiciede treatments 2 and 3 control very well broad leaf weeds,without detectable effects over grass weeds. In lupin and chickpeathehighestgrainyieldwas recordedunder the treatment4.For therest of legumes, the highest grain yields were recorded under thetreatment 2, that was the second highest grain yield for lupin andchickpea. Phytotoxicity sympthoms were detected when thetreatments2and3wereapplied.

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P135–S14 Nitrogenavailabilityfrompeasandfababeansaspre-cropstobroccolifollowedbylettuce,inNorwegianfieldtrialsSeljåsenR.1,TorpT.2andVågenI.M.11 - Norwegian Institute of Bioeconomy Research, Division of food production andsociety,Norway2)-DepartmentofHorticulture,Reddalsveien215,4886Grimstad,Norway.Meth:Organicfieldtrial, ‘spilt-splitplotdesign’with4blocks.Wholeplots (spring 2014) had legumes (pea or faba beans), and sub plots(autumn 2014) had 4 autumn soil treatments with combinations oflegume residue incorporation and cover crop. The sub-sub plots(spring2015)werewithandwithoutadditionalmanurefertilization.Res:Therootbiomassofbothlegumeprecopshadequalnitrogen(N)concentration,buttotalrootbiomasswastwiceashighforfavabeansas for peas (5.08 vs. 2.41 kgm-2). Fava bean pre cropwith biomassincorporationwithoutcovercropgavethehighestbroccoliyield(4.10t ha-1) comparedwith pea pre cropwith biomass incorporation andnocovercrop(2.44tha-1).Alsothelastcropintherotation,lettuce,had94%higheryieldafter favabeans (6.6 tha-1) compared topeas(3.4tha-1).RyeascovercropefficientlyassimilatedandconservedNduringwinter, shown by a 4 to 5-fold reduction in soil NO3-N, andnearly 2-fold reduction in soil N-min levels, compared to open soil.Additionalmanureapplicationaffectedcropyield,with3-and2-foldincreaseinbroccoliandlettuce,respectively.Conc:Favabeansasprecropresultedinhigheryieldsofbroccoliandlettucethefollowingseasons,comparedtopeas.Thiswasexplainedbytwiceasmuchrootbiomassforthatcrop

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P136–S14 EvaluationofagronomicpracticesonproductionofClearfieldredlentilinAlberta,CanadaBownessR.2,DubitzT.2,OlsonM.A.1,HoyC.1,HenriquezB.1,BandaraM.5,KrugerA.5,PaulyD.3,MiddletonA.3,PfiffnerP.3,GillK.4,PettyjohnJ.P.41-AlbertaAgricultureandForestry,Edmonton,Alberta,Canada2-AlbertaAgricultureandForestry,Lacombe,Alberta,Canada3-AlbertaAgricultureandForestry,Lethbridge,Alberta,Canada4-SmokeyAppliedResearchandDemonstrationAssociation,Falher,Alberta,Canada5-AlbertaAgricultureandForestryFoodandBio-IndustrialCropsBranch,Alberta,CanadaRed lentil (Lens culinaris L.) is an important nutritional globalcommodity. It is the largest acreagepulse crop grownon theprairiesandCanadaistheleadingexporter.Aslentilfixesnitrogenfromtheair,it is a valuable crop for rotation. Field trials were conducted acrossAlbertafrom2012-2015toidentifythebestmanagementpracticesforClearfield red lentil. Treatments included a range of nitrogen rateapplications combined with the presence or absence of rhizobialbacteria inoculation, five different seeding densities and the use ofseveral imidazolinone herbicide formulations. Plotswere assessed foragronomic and phenological traits. Results were variable acrosslocationsandyears,howevertheresultsindicatedthatredlentilgrowswell in Alberta. Nitrogen application improved plant growth at lowrates,butnegativelyaffectednodulationandseedyieldatlevelsabove30kgNha-1.Theoptimumseedingdensityrangedbetween120to160plants m-2 but the yield response above 60 plants m-2 was notsignificant.Ingeneralallherbicideseffectivelycontrolledtheweedsbutsome formulations caused a decrease in nodulation and seed yield.Consideringthesuccessoftheredlentilstudies,Clearfieldredlentilisagreat rotational option forAlberta producerswhenproper agronomicpracticesarefollowed.

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P137–S14EffectofforagelegumesmanagementmethodsonvariationdynamicsofmineralnitrogenintheLithuania’sCambisolArlauskienėA.Joniškėlis Experimental Station of the Lithuanian Research Centre for AgricultureandForestry,LithuaniaThe study was aimed to determine of perennial forage legumes(TrifoliumpratenseL.,MedicagosativaL.)andtheirmixtureswithfestulolium (x Festuliolium), used as mulch, and their effect onmineral N variation in the clay loam Cambisol. The experimentswere conducted in 2007-2012 in the crop sequence: perennialgrasses–winterwheat–wintertriticale.Managementmethodsoftheabovegroundmassofperennial grasseswere: removed fromthe field (cut twice);mixedmanagement (first cutwas removedfrom the field, second and third cuts –mulched);mulching (cutfour timesandmulched). ThemineralN content variation in thesoil depended on the incorporated mass of perennial grasses(mixed management), its C:N and lignin:N ratios (mixedmanagement and mulching) and meteorological conditions (allmethods). The compatibility of N release from incorporatedorganic matter and cereal N demand was established not everyyear.Infavourableyears,foragelegumesandtheirmixtureswithfestulolium significantly increased mineral N in the soil for twoyears in spring. The effect ofmulchwasmore prominent in thesecondyearofcerealgrowing.Rainysummerandautumn ledtoincreasedmineralNcontentafter incorporationof legumes totalmulch. During the non-vegetation period of plants, mineral Ncontentinthesoilcanbereducedbyapplyingmixedmanagementmethodorbyusingmixturesofforagelegumesandfestuloliumasmulch.

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P139–S14 SeedyieldstabilityassessmentandQTLmappingforyield-relatedtraitsinnarrow-leafedlupin(LupinusangustifoliusL.)GórynowiczB.,KrocM.,ŚwięcickiW.InstituteofPlantGenetics,PolishAcademyofSciences,Poznan,PolandLupins are characterized by high potential of seed yield production,especially with reference to protein content. Unfortunately yieldstabilityoflupinsisvariableinfluencingtheirgrowingarea.Seedyieldand its componentsarequantitatively inheritedbutalsoaffectedbyboth agricultural andweather factors. The aim of this studywas toassessselectednarrow-leafed lupincultivarsgathered in thecurrentPolish National List of Varieties, regarding their yield stability.Moreover regions of Lupinus angustifolius genome governing theexpressionofseedyieldcomponentswereidentified.In the assessment of seed yield size and quality 10 traditional andunbranched cultivars of narrow-leafed lupin were included. Fieldexperimentswereconductedin2011-2014inlocationswithdifferentsoilandweatherconditions.Populationof89RILsinF8wasassessedregarding four seed yield components (number of pods per plant,numberofseedsperplant,yieldand1000-seedweight)during4-yearfieldtrial(2012-2015)toidentifyQTLs.Asaresultof thisstudywe identifiedthemoststable lupincultivarsregarding yield, which are Boruta and Graf. Less stable were Kalif,Kadryl and Regent,while asmost unstablewe found Zeus, Neptun,Sonet, Bojar and Dalbor cultivars. Moreover QTLs consistent acrossyearsresponsibleforseedyieldanditscomponentswereidentified.

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Session 15, parallel: Frontiers in legume breeding(cont.)-RoomArrábidaIIIChairedbyPaoloAnnicchiarico(CREA,Italy)

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OralCommunications14:30-14:40Oral–S15GenomicbasedplatformforchickpeabreedingprogramattheUniversityofSaskatchewanDeokarA.,Tar'anB.CropDevelopmentCentre/DepartmentofPlantSciences,UniversityofSaskatchewan.51CampusDr.,Saskatoon,SK,Canada,S7N5A8bunyamin.taran@usask.caImportant breeding objectives of chickpea consist of traits withdifferent levels of genetic complexity. For simple traits such asherbicideresistance,growthhabitandearlyflowering,MAShasbeeneffective and allows selection for desirable breeding lines at earlygeneration, thus, only selected lines continue for agronomicevaluationat latergenerations. TheuseofMAS for improvingmorecomplex traits in chickpeas, however, had very limited success.Genomicselection(GS)hasbecomeanimportantmethodofapplyingmolecular markers for selection of complex traits in plants. WeappliedGS in chickpeaandevaluated theeffectofmarker selectiononpredictionaccuracy.Apanelof281germplasmandelitelineswasevaluatedatmultilocationsin2014andgenotypedbyGBSgeneratingatotalof4,524SNPs(MAF>0.05).Genomewideassociationanalyseswere done to examine the genetic architecture of the traits beingtested. Best linearunbiasedestimates(rrBLUP)werecalculatedandinputintothegenomicpredictionmodelfordaystoflowering,daystomaturity, resistance to ascochyta blight, seed weight, seed ironconcentration and grain yield. The effect of training population sizeonpredictionaccuracywasevaluatedusingavalidationsetcomprisedof randomly selected lines and training sets of 10-90% totalpopulation. The validation set was created by randomly samplingbreeding lines without replacement. The correlation accuracy withvarying training population sizes ranged from 0.20 for seed ironconcentration to 0.80 for 1000 seed weight. For seed weight, thepredictionaccuracyincreasedfrom70%to80%whenthesizeofthetrainingpopulationincreasedfrom28to84lines;furtherincreaseofthetrainingpopulationsizedidnotsignificantlyincreasetheaccuracy.

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14:40-14:50 Oral–S15Fababeanlinesdifferintheircontributionaspollendonortocross-fertilizedseedBrünjesL.,LinkW.Georg-August-Universität,DepartmentofCropSciences,PlantBreeding,Von-Siebold-Str.8,37075Göttingen,GermanyFaba bean (Vicia faba L.) is still a marginal crop in Germany due toinsufficient yield level and yield stability. In this study, we aim atincreasing both traits by increasing shares of heterosis in syntheticvarieties. Inpartiallyallogamouscropssuchasfababean,theshareofheterosis in a synthetic can be increased by higher degrees of cross-fertilization.Thisdegree isacentralparameter inyieldpredictionandgenerallydefinedastheratiobetweencrossedseedandallseed.Whenusingthedegreeofcross-fertilizationtopredictinbreedingandshareofheterosis, genotype-specific degrees of cross-fertilization areconsidered while assuming that all genotypes contribute the samepollendosetothecross-fertilizedseeds.However,weexpectfababeangenotypestodifferinpaternalmatingsuccessassumingthatgenotypeshavedifferentsuccessratesaspollendonors.Toquantifythevariationof both, the degree of cross-fertilization and of the paternal matingsuccess,wehaveassessedtheseparametersinapolycrossofeightfababean lines, each grownwith 64 individuals. Ten seeds per plant havebeenanalyzedusingeightSNPmarkers inorder to identify the fathergenotype of each seed. Based on these results, the degree of cross-fertilizationvariedbetween28and63%, thepaternalmatingsuccessbetween 8 and 19 %. We will include these differences in yieldpredictionsofsynthetics, tobettermanageandexploitheteroticyieldincreaseinfababean.

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14:50-15:00 Oral–S15Assessingandovercominggenetictrade-offsinbreedinggrazing-tolerantalfalfaPecettiL.,AnnicchiaricoP.Council for Agricultural Research and Economics – Research Centre for FodderCropsandDairyProductions(CREA-FLC),Lodi,ItalyGrazing-tolerant alfalfa is an important asset for sustainableintensification of Mediterranean-climate crop-livestock systems,butselection ischallengedbymarkedcold-seasondormancyandprostrate habit of tolerant material. This study assessed indifferent genetic backgrounds the size of genetic variation andgenetically based trade-offs for various key traits and theirimplicationsforselection.Some432clonedF1progeniesissuedbyfourcrossesbetweenerect/littledormantandprostrate/dormantgenotypes were evaluated for dry-matter (DM) yield and finalpersistence under continuous, intense sheep grazing for threeyears. Both DM yield and persistence had negative geneticcorrelationwitherectplanthabit(rgrange:–0.31to–0.87),whilepersistencewas inversely related also to cold-season growth (rgrange:–0.33to–0.73).CorrelationsofthesetraitswithearlyDMyieldundermowing,plantdiameterand leafletareawereerraticor nil. DM yield under grazing and persistence showed largegenetic variation (CVg range: 33.3 to 57.8%), and about 50%greaterwithin-thanbetween-crossvariance.Morphophysiologicaltraitshadlowergeneticvariationandevengreaterrelativesizeofwithin-cross variance. Selecting grazing-tolerant cultivars canexploit largegeneticvariationbutrequiresextensivewithin-crossgenotypeevaluation toproducematerialwithmodest dormancyand relatively erect habit. Marker-assisted selection needs beexploredtofacilitatethistask.

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15:00-15:10 ORAL–S15EnhancingthenutritionalqualityoffieldpeaWarkentinT.D.1,ArganosaG.A.1,BangarP.1,LiuX.1,ShunmugamA.2,DelgerjavT.3,DiapariM.4,RehmanA.5,JhaA.B.1,AshokkumarK.6,PurvesR.W.1,ThackerP.7,Tar’anB.1BettK.E.1,andGlahnR.P.81-CropDevelopmentCentre,UniversityofSaskatchewan,Saskatoon,SK,Canada2-AgricultureandAgri-FoodCanada,Saskatoon,SK,Canada3-BayerCropScience,Inc,Saskatoon,SK,Canada4-AgricultureandAgri-FoodCanada,London,ON,Canada5-Cargill,Inc.,Aberdeen,SK,Canada6-DepartmentofPlantBiotechnology,TamilNaduAgriculturalUniversity,Coimbatore,TN,India.7-DepartmentofAnimalandPoultryScience,UniversityofSaskatchewan,Saskatoon,SK,Canada8-USDA-ARS,CornellUniversity,Ithaca,NY,USAField pea seeds, like those of other pulse crops, are rich in protein,slowly digestible carbohydrates, and fiber. To further enhance theirnutritionalvalue,researchisinprogresstoincreasetheconcentrationandbioavailabilityofkeymicronutrients.A100gdryweight servingoffieldpeasuppliedasubstantialportionoftherecommendeddailyallowanceofFe,Zn,andSeforadults.Associationanalysis identifiednine single nucleotide polymorphisms (SNPs) associated with Feconcentration, and 2 SNPs associate with Zn concentration in pea.Field pea lines were identified with a 60% reduction in phytate-phosphorus concentration in seeds controlled by a single recessivegene mapped on pea chromosome 3. Iron bioavailability, assessedusingahumancellcultureassay,ofprogenyarisingfromcrosseswiththelowphytatelinesdisplayedupto3-foldgreaterironbioavailabilitythan thatof theirprogenitor. In somecases, ironconcentrationandlutein concentration were positively correlated with ironbioavailability. Total tract apparent availability of phosphorus andbone strengthwere higher for broiler chicks fed a low phytate peadietthanforbirdsfedanormalpeadiet

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15:10-15:20 ORAL–S15MachinelearningapproachesallowfordeterminationofimportantroottraitsandenhancedifferentiationoflegumescultivarsZhaoJ.1,GaonkarB.2,BodnerG.3,RewaldB.11 - Department of Forest and Soil Sciences, University of Natural Resources and LifeSciences,Vienna(BOKU),Peter-Jordan-Straße82,1190Vienna,Austria.2-CenterforBiomedical imagecomputingandAnalysis,DepartmentofBioengineering,UniversityofPennsylvania,3600Marketstreet,Philadelphia,PA19104,USA.3-DivisionofAgronomy,DepartmentofCropSciences,UniversityofNaturalResourcesand Life Sciences, Vienna (BOKU), Konrad Lorenz-Straße 24, 3430 Tulln an der Donau,Austria.Plantphenotypingintendsmeasuringcomplextraitsrelatedtogrowth,yield, and adaptation to stress at different macroscopically scales ofplant organization. Especially the modification of root systemarchitecture (RSA) could contribute to improvements of desirableagronomic traits;RSAwasdescribedkey toasecondgreenrevolutionimproving resource use efficiency of crops. Machine learningapproaches are promising statistical tools for variable selection andgroup classification—reducing the workload for humans, but alsoensuring objectivity and consistency in analyses. However, newapproaches overcoming the difficulties of applying machine learningwith unbiased root traits importance measure on small size samplesand powerful classification methods are needed to improve theanalysesofmanuallyandimageanalysis-derivedphenologicaldatasetsandidentifycandidatecultivarsforwideragriculturaluseand/orfuturebreeding efforts. Thus,we developed algorithms identifying themostdiscriminative root traits of three legume specieswith random forestmodels and differentiated cultivars and species with support vectormachineanalysisbasedonroottraits.Interestingly,discriminativeroottraitswereuniqueineachspecies.Themachinelearningapproachcouldaccuratelyidentifycultivarswithrootsystemarchitecturessuchasdeeporshallowrootingwhichcanbeusedfor future breeding. Funded by FP7 grant agreement no 613781(EUROLEGUME).

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15:20-15:30 Oral–S15AninternationalnetworktoimprovemungbeanbreedingandproductionMadhavan-NairR.1,SchafleitnerR.2,DouglasC.3,AlamMd.M.4,GargA.P.5,ShweT.6,HuttnerE.71-WorldVegetableCenter,SouthAsia.2-WorldVegetableCenter,HQ.3-DepartmentofAgricultureandFisheries(DAF).4-BangladeshAgriculturalResearchInstitute(BARI).5-IndianInstituteofPulseResearch(IIPR).6-MyanmarDepartmentofAgricultureResearch(DAR).7-AustralianCentreforInternationalAgriculturalResearch(ACIAR).Mungbean[Vignaradiata(L.)R.Wilczekvar.radiata]isanimportantfood legume insouth,eastandsoutheastAsia,where90%ofglobalproduction currently occurs. More than 6 million hectares arecultivated, producing up to 3 million metric tons of grain that isconsumeddirectlyasdhal,beansproutsornoodles.TheWorld Vegetable Center holds the world’s largest collection ofVignaspecies,with11,591accessions.RecentlytheCenterdevelopedamungbeancorecollectioncomprising1,481accessionsandamini-core comprising 296 accessions. The core collection was developedbased on phenotypic characterization, while the mini-core wasdeveloped by molecular characterization using 20 single sequencerepeat (SSR) markers. Intra-specific and inter-specific hybridizationmethods are being utilised to tackle major problems in mungbeansuch as mungbean yellow mosaic disease, Cercospora leaf spot,powderymildew, and bruchids. Also variation for nutritional qualityaswellassproutingabilityinmungbeanarebeingexplored.TheInternationalMungbeanImprovementNetworkanditsoutcomeswillhelpunlockthepotentialofmungbeantoimproveproductivityoftropicalagricultureinAustraliaandAsia.TheprojectisaninitiativeofACIAR,DAF,andtheWorldVegetableCenter,andwillinitiallypartnerwith Bangladesh, India andMyanmar. The objectives of the projectare to develop coordinated pre-breeding capability; conduct anextensive phenotypic characterisation of the mini core collection;screenforresistancetokeybioticandabioticstresses;genotypethemini-coreathighdensityand identifymarker traitsassociationsandsupporttheseactivitieswithanintegratedplantbreedinginformationsystem.

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Session 16, parallel: Advances in legume agronomy(cont.)-RoomArrábidaIVChaired by Fred Stoddard (Univ. Helsinki, Finland) andClaudioPorqueddu(CNR,Italy)

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Oralcommunications14:30-14:40 Oral–S16EcosystemservicesprovidedbylegumesandexploitedbystakeholdersinthreefrenchterritoriesCorre-HellouG.1,MaulineM.1,NaudinC.1,PoretJ.1,SorinS.2,PelzerE.3,MédièneS.3,JeuffroyM.-H.3,BazotM.3,JournetE.-P.4-5,LeclouxE.5,JustesE.5,LarribeauA.6,GarnaudD.71 - LEVA, Univ. Bretagne Loire, Ecole Supérieure d’Agricultures (ESA), 49007Angers,France2-TerrenaInnovation,44150Ancenis,France3 - UMR Agronomie INRA, AgroParisTech, Université Paris Saclay, 78850Thiverval-Grignon,France4-LIPM,UniversitédeToulouse,INRA,CNRS,Castanet-Tolosan,France5 - AGIR, Université de Toulouse, INRA, INPT, INP- EI PURPAN, Castanet-Tolosan,France6) - Qualisol, 82102 Castelsarrasin, France 7 S.C.A de déshydratation de laHauteSeine,21450BaigneuxlesJuifsLegumes offer a wide array of services to be valued inproduction systemsand supply chains: productionof protein-richseedswithinterestingnutritionalpropertiesforbothfoodand feed; beneficial preceding crop effects through therestitution of biologically fixed N and the time/spacediversification of cropping systems. However the highvariabilityofperformanceshindersanticipatingandexploitingsuchservices throughadaptedcropping systemmanagement.Today, stakeholders are lacking local references for diverselegume species and insertion modes. Hence approaches toinsert more legumes in territories need to be assisted byidentifying / characterising 1) the contrasted actorsexpectations, 2) services obtained by farmers in real farmcontext,3) themainvariability factorsand levers tooptimallyexploitservices,andbysharingresultswithactors.In3frenchterritories(PaysdelaLoire-PDL,Burgundy-B&Midi-Pyrénées-MP)observatorieshavebeen setup inpartnershipwith farmcooperatives.Severalspeciesandinsertionmodesselectedbylocalactorsarebeingstudied:spring/winterlupinassolecropor intercroppedwithtriticale (PDL);alfalfaandspringpea(B),soybean, and lentil as sole crop or intercropped with wheat(MP).A2-year followupof the legumeand thenext crophasbeenperformed in2015-16and is repeatedonotherplots in

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2016-17.Performancesvariabilityhasbeenanalysedinrelationwith(a)bioticlimitingfactorsandfarmerspractices.14:40-14:50 Oral–S16Quantificationofnitrogenfluxesandexplanatoryplanttraitsduringatwo-yearlegume-cerealrotationGuinetM.,VoisinA-S.,NicolardotB.UMR Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, F-21000Dijon,FranceIn the context of agroecological transition, the reintroduction oflegume crops should play a key role in cropping systemsustainability by allowing a reduction of nitrogen (N) inputs. Butfew references are available concerning the agronomical andecological services provided by a wide range of legume crops,particularlywithincropssuccessionscale.Thus,themainobjectiveof our study is to quantify the N fluxes during and after thelegume crops taking into account 10 legume crops (peas, lupin,faba bean, soybean...). Our experiment consists in i) quantifyingsymbioticNfixationdependingontheamountofsoilinorganicN,themineralisationofNpresentinlegumecropresiduesaftersoilincorporation and N losses outside of the soil-plant system(leaching,emissionofnitrousoxide),ii)identifyingplantbiologicaltraits associated to N fluxes. Thus, different N fluxes werequantified during a two-year field experiment, i.e. the first year(2014) legumecropswere implantedand followedbywheat thesecondyear (14-15)after incorportationof legumeresidues.Thisexperiment will be repeated in 2016-2017.The first resultsconcerning legumepre-crop effectswill be presentedduring thecongress, considering the effect of preceding legume crops onwheat seed yield and N content. This effect will be related tolegumeaerialandbelowgroundresiduesthatwerecharacterizedbytheirbiomass,C/Ncontentandpotentialmineralization.

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14:50-15:00 Oral–S16Cerealsascompanioncropstoimproveweedcontrolandoverallproductivityincereal-grainlegumeintercrops:caseoflupinCartonN.,PivaG.,NaudinC.,Corre-HellouG.URLEVA-ESA,55RueRabelais,49007Angers,FranceLow and unstable yields due to abiotic and biotic factors (notablyweeds)aremajorobstaclestoabroaderadoptionofgrain legumes inEuropean agricultural systems. There is a need to design alternative-cropping practices for grain legumes in order to secure theirproductivityandimproveweedcontrolwhilereducingherbicideuse.Intercropping, the growing of two or more species together on thesame area of land at the same timemay be away to decrease yieldvariability and to increase competitive ability againstweeds. A cerealcropcanbeseenasacompanioncropprovidingservicestothelegumeincereal-legumeintercrops.We illustrate the benefits of intercropping legumes with a non-fixingcroptocontrolweedsandincreaseoverallproductivityascomparedtograin legume sole crops. Themechanisms explaining the benefits arepresented,witha focuson thedynamicsofnitrogenand light sharingbetween legume crop, non-legume crop and weeds. We alsodemonstratetheimpactofplanttraitsoninteractionsusingcontrastedcombinationsofspeciesandcultivars.Thisworkshowstherelevanceofusingtraitcomplementaritieswhendesigningintercrops.Theapproachis illustrated with winter white lupin (Lupinus albus L.), which is wellknown for its high yield variability and low competitive ability againstweeds.Lupin-cerealintercropsarecomparedwithlupinsolecropsbothincontrolledconditionsandfieldexperimentsinWesternFrance.

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15:00-15:10 Oral–S16IntercroppinglentilwithspringwheattoimproveproductivityandincomeinorganicfarmingViguierL.1,2,BedoussacL.2,3,JournetE.P.2,JustesE.21-CooperativeQualisol,F-82100Castelsarrasin,France2-INRA,UMR1248AGIR,F-31320Castanet-Tolosan,France3-ENFA,F-31320Castanet-Tolosan,France4-CNRS,UMR2594LIPM,F-31320Castanet-Tolosan,FranceThe food legume lentil is attracting growing interest among organicfarmers. However, its productivity is low and very variable notablydue to its lodging sensitivity. We analysed the functioning andperformances of lentil-spring wheat intercrops (IC) for yieldimprovement/stabilization. An organic field experiment wasconductedat INRA-Toulouse,with4 lentilcvs.and2ofspringwheatgrownassolecrops(SC)andICat4seedingratios(67/33%,100/17%,100/33% and 100/50% for lentil and wheat resp., compared to SCdensity). Total IC grain yieldwas 1.24 t/ha andwashigher than themeanyieldof therespectiveSCs,higherthanthatof lentilSCs (0.87t/ha)andsimilartothatofspringwheatSCs(1.29t/ha).Thisconfirmsthe interest of IC in organic farming to improve yield due to thespecies complementarity for use of available resources. Lentilproduction was often lower in IC than in SC as a result of wheatcompetition.Hencetofavouryieldoflentil,themostprofitablecrop(3-4 times higher price),wheat densitymust remain low. Evenwithwheat density as low as 17% lentil lodgingwas reducedwhichmayincrease theamountof lentilharvested in ICvs. inSC.Thus lentil ICcouldbeanefficientsystemtoincreaseorganicfarmersnetincomeincomparison to classical lentil SC. "Genotype by density by species"interactionswereobservedacrossICcombinations,callingforfurtherstudyofthedifferentcultivarsresponsesinordertodesignoptimizedICsystems.

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15:10-15:20 Oral–S16Diversificationandintensificationofusadrylandcroppingsystemsusingautumn-sownwinterpeaGuyS.O.1,McGeeR.J.2,LauverM.A.11 - Department of Crop and Soil Sciences,Washington State University, Pullman,WA,USA.sguy@wsu.edu2-GrainLegumeGeneticsandPhysiologyResearchUnit,USDA-ARS,Pullman,WA,USAIntheUSAstatesWashington,Oregon,Idaho,andMontana,5.3millioncroppedhectaresaveragelessthan450mmannualprecipitation.Therearefewalternativecropstowinterwheatinlowrainfall,butthereisastrong interest in economically viable rotation crops where fallow ispracticed. Autumn-sown peas (ASP) can now be marketed as a foodquality allowingpotentialASPproduction in low rainfallwinterwheatareas. Spring-sown peas are lower yielding than ASP by 30-50% andmature later. Early maturity is important when crops mature underterminal drought and heat stress. Emergence can be a problem forwinterwheatseededdeeptoreachmoisture.ASPconsistentlyemergesfrom15cmdepthsormoreandforcesthroughmostcrusting.ImprovedwinterhardinessinASPcanrivalwinterwheat.CurrentASPproductioninthedryareasincludescovercropandfeeduses,andcontractsareinhighdemand.FoodqualityASPvarietieswillsoonbereleasedbyUSDA-ARS and private breeders that can be sold to open markets andhectarageshouldnotbelimitedbycontracts.ASPconductsbiologicalNfixation, is a low energy and greenhouse gas emissions crop, willinterruptweed,diseaseandinsectcycles,hasalowwateruse,andcanflourish under current cropmanagement conditions. Spring sownpearotatedwithwheatinhigherrainfallareasrevolutionizedthatcroppingsystem. Similarly, ASP is poised to diversify and intensify cropproductionespeciallyinthelowerrainfallregion.

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15:20-15:30 Oral–S16Quantitativeanalysisoftherootdistributioninafababean-wheatintercroppingsystembyFouriertransforminfrared(FTIR)spectroscopyStreitJ.,MeinenC.,RauberR.Georg-August-UniversityGoettingen|Department forCropSciences|DevisionofAgronomyIntercropsoftendemonstratehigheryieldscomparedtotheirsolecrops due to complementary resource use e.g. in light ornutrients. Another reason for this effect could be a differingrooting system of both intercropping partners and therefore ashift in rooting patterns resulting in root mass overyielding. Tostudy these species-specific rooting patterns, roots have to beidentifiedtospecieslevel.Inthisstudy,Fouriertransforminfrared(FTIR) attenuated total reflection (ATR) spectroscopy wassuccessfullyused todiscriminate specie specific rootproportionsinafababean-wheatintercroppingsystem.Eightwinterfababeanlines (Vicia faba L.) and one winter wheat cultivar (Triticumaestivum L.) grown in ina field trialofpure standsandmixtureswereinvestigatedinregardtotheirdifferencesinrootdistributiondownto60cmsoildepth(4repl.).FTIRspectroscopywasusedtoanalyze absorption spectra of dried and ground roots. Roots ofpurestandswereused toprepareartificial samplesof fababeanandwheat rootmixtures to calibrate and validate a FTIRmodelwhich predicted the species proportion in root mixtures. Rootabsorption spectra showed species specific peak distributions.Fababeanandwheatrootspectrawereclearlyseparatedbytheclusteranalysis.Preliminaryresults indicatearootpartitioningofboth species and a tendency of higher root biomass inmixturesthaninsolestands(overyielding).

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Session17,parallel:Legumesandenvironment(cont.)-RoomArrábidaIIIChairedbyChristineWatson(SRUC,UK)

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OralCommunications16:30-16:40 ORAL–S17Soilnitrogenfertilityandnitrogenacquisitioninfababean–long-termsystemeffectsCarlssonG.,ErnforsM.,MårtenssonL.M.,JensenE.S.SwedishUniversityofAgriculturalSciences,Alnarp,Sweden.Nitrogen(N)fertilizationoflegumesreducessymbioticN2fixation,butknowledge on the long-term effects of N fertilization in non-legumerotationalcropsonthesymbioticperformanceofgrainlegumesgrownwithoutfertilizerNislimited.Inthisstudy,fababean(ViciafabaL.)wasgrown in a six-year conventional crop rotation with cereals, oilseedrapeandsugarbeet,attheSITESLönnstorpfieldexperimentalstationin southern Sweden. The experiment started in 1994 and includedthreelevelsofNfertilizationtotheothercropsthanfababean:0(N0),85 (N2) and 160 (N4) kg N/ha. Fababean were sampled in 2011 and2012, after three full crop rotations, for determination of grain andstraw yield, N accumulation from soil and symbiotically fixedN usingthe natural 15N abundancemethod. The average fababean grain andstrawyieldsacrossNlevelsandyearswere4.6and2.9metrictonsperha,respectively,andtherewasnosignificanteffectofNfertilizerlevelto other crops on fababean N concentrations. The total fababeanaccumulationofsoilNincreasedwithapproximately40kgNperhaintheN4systemcomparedtotheN0system,whilethepercentNderivedfromN2fixationwasreducedfrom67(N0)to54(N4)%.TheresultsarediscussedinthecontextsofNuseefficiencyatthecroppingsystemandglobalNcyclelevels.

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16:40-16:50 Oral–S17Impactoforganicpracticesongrowth,yield,greenhousegasemissionsandbiologicalnitrogenfixationbythreelocalpeaandfababeanlandracesSavvasD.1*,PappaV.A.1,YfantopoulosD.1,KarkanisA.2,TravlosI.1,BebeliP.J.1,NtatsiG.1,BilalisD.11-DepartmentofCropScience,AgriculturalUniversityofAthens,Athens,Greece.2 - Department of Agriculture Crop Production and Rural Environment, University ofThessaly,Greece.Legumes constitute an essential component of rotations in organicsystems due to their ability to provide plant available N2 toagriculturalecosystems.However,thereisageneralneedtoincreasegrain legume protein production in Europe so as to meet theincreasing demand while reducing resource utilization, therebycontributing to mitigation of global climate change. A field-basedexperimentwithpeaandfababeanwascarriedoutinafieldcertifiedfor organic agriculture from 11/2014 to 11/2015 within theframeworkofEUROLEGUME.Theexperimentwas laidout inasplit-plotdesignwith2maintreatments(conventionalandorganicfarmingsystem) and 4 sub-plots permain plot corresponding to 4 differentpea and faba bean cultivars (peas: Onwards Amorgos, Andros andSchinousa; fababeans:Aguadulce,Andros, Lefkada,Mani). Standardinorganic fertilizerandsheepmanurewereused intheconventionaland the organically treated plots, respectively. The aim of theexperimentwas to test the performance of each cultivar in organicfarmingcropsascomparedtoconventionalcropping,intermsofGHGemissions and biologicalN2-fixation efficiency. The results indicatedsignificantdifferencesincumulativeN2OfluxesbetweenpeacultivarswithSchinousaproducingthehighestN2Oamountsand‘Andros’thelowest. Also, Lefkada showed similar cumulative fluxes in bothsystems.Furthermore,SchinousaexhibitedthelowestBNFefficiencyandLefkadathehighestinbothsystems.

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16:50-17:00 Oral–S17EffectofsimulatedhailtreatmentonyieldlossinchickpeaMcPheeK.NorthDakotaStateUniversity,USAHail can have devastating effects on crops and dramatically reduceproductivity and quality. This study evaluated the amount of damageandyieldlossfromsimulatedhail.Thisresearchwillaidcropadjusterstoquantifyhaildamageinchickpeafieldsatcertaingrowthstages.CDCFrontier was grown at the Carrington Research Extension Center inNorthDakotain2015.Fivetreatmentlevels,untreated,25,50,75,and100%,wereappliedatvegetative, first flower,and latebloomgrowthstages. Treatment levels were established based on canopy heightbefore treatment to remove 25% of canopy height for the 25% leveland 40% of canopy for the 50, 75 and 100% treatments. Individual30x30 cm samples of plant material were collected after initialtreatment to determine biomass reduction and assess damage onindividual plants. Post-harvest data, seed yield, one thousand seedweight,andseedsizedistribution,werecollectedtocorrelatewiththelevelofdamage.Averageseedyieldswere2482,1719,and1344kg/hafor treatments at the vegetative, first flower, and late bloom stages,respectively.Theaverageseedyieldforthefiveinjurylevels,untreated,25, 50, 75, and100%,were2482, 2102, 1649, 1536, and1476 kg/ha,respectively. Hail events later in the season with increased level ofdamagereducesseedyieldandcorrelationanalysesshowedthatplantinjury data will be useful in establishing procedures to aid insuranceadjustment.

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17:00-17:10 Oral–S17Meta-analysisoftheeffectsoflegumecompanionplantsonweedcontrolandyieldofthecashcrop.VerretV.Unitémixtederecherched'Agronomie-Inra-AgroParisTech-Versailles-GrignonCompanionplant(CP) intercropping involvesgrowingacashcropwithanotherplantthat isnotharvested,thatdeliversservicestothe crop and the environment. CP can compete with weeds forlightandnutrients,butmayalsocompetewiththecrop.Here,wereviewed 476 observations from 34 studies of intercrops of anannualcashcropandalegumeCP.WeexploredwhetherlegumeCP intercroppingcancontrolweedswhilemaintainingcropyield.Yield and weed biomass ratios were analyzed as responsevariablesinlinearmixedeffectmodelswiththetypeofcashcrop(straw cereals, maize or other crops) and the methods used toestablish the CP (living mulch, synchronized sowing or relayintercropping)asexplanatoryvariables.52% and 34% of the intercrops resulted in lower weed biomassand higher yield (win-win) compared to non-weeded (NW) orweeded(W)controltreatments(CT)respectively.13%and27%ofthecaseshadahigherweedbiomassassociatedwithaloweryield(lose-lose)comparedtoNWCTandWCT,respectively.Inaverage,companionplantshadnosignificanteffectoncashcropyield,butsignificantlydecreasedweedbiomassby56%relatively toNWCTand42%relativetoWCT.Inparticular,intercroppedmaizeyielded37%morethanNWCTthankstogoodweedsuppression.Theuseof legume CP generally enhance weed control without reducingcrop yield, but the conditions giving rise to win-win situationsshould be explored further to encourage the spread of thistechnique.

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17:10-17:20Oral–S17AtoolintegratingandsharingknowledgetoselectlegumespeciesforoilseedrapeintercroppingMédièneS.,VerretV.,FelixJ.,Valantin-MorisonM.UMR211Agronomie,INRA-AgroParisTech,78850Thiverval-GrignonLegumes companion plants can contribute to design cropping systemslessreliantonsynthetic inputs,astheycandeliverecosystemservices:e.g.nitrogensupply,weedregulation.Researchershavetoidentifyandbring new knowledge to farmers to help them designing innovativesystems based on intercrops. Our work focuses on oilseed rapeintercroppedwithfrost-sensitivecompanionplants.Wepresent here the steps to elaborate a tool integrating and sharingknowledge to select candidate species for oilseed rape intercropping,accordingtotheirabilitytodeliverregulationandprovisionservices:•censusofexistingtoolsandpotentialusers’needs•identificationofplanttraits(bothlegumeandnon-legumeplants)andagro-pedo-climatic characteristics that enable the realization ofprocesses leading to services delivery (by literature review andparticipatory workshops between extension advisors, technicalinstitutesandresearchers)•building a multi-attribute aggregation tree (DEXI software) toaggregate plant traits and agro/pedo/climatic characteristics into aratingofservicesdelivery•compiling20traits inadatabase, inadditionto13agro-pedo-climaticelementsaskedtotheuser,usedasinputvariablesintheDEXItree.Asoutput,thetoolclassifiescompanionplantspeciesaccordingtotheirpotential to deliver ecosystem services and explains relationshipsbetween plant traits, services delivery and interactions with theenvironmentinapedagogicalpurpose

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17:20-17:30Oral–S17Nitrousoxideandmethanefluxesfromacowpea-broccolicroprotationunderconventionalandorganicmanagementpracticesSánchez-NavarroV.,ZornozaR.,FazA.,andFernándezJ.A.UniversidadPolitécnicadeCartagena,ETSIA,Cartagena,SpainThisstudywascarriedout inacowpea-broccolicroprotationwiththeaim of evaluating the effects of applying conventional and organicfertilizers on greenhouse gas emissions (N2O and CH4) and theirrelationshipwith soil properties. Two cultivars of cowpea (Black-eyedpea and Grey-eyed pea) were grown within a randomized completeblock study with 4 replications in plots of 10 m2 that rotated withbroccoli. Gas samples were taken at different times (0, 30 and 60minutes) once a week using the static gas chamber technique. Soilsamples (0-30 cm)were taken from each plot at the end of the cropcycletomeasuresoilorganiccarbon(C),totalnitrogen(TN),NO3-andthe enzyme activities. The results obtained from the cumulative N2Ofluxes did not show a clear pattern of N2O emission as a function ofmanagement practice or cultivar.However, the cumulative CH4 fluxesforbothcowpeacultivarswerehigherunderconventionalmanagementthanunderorganic,withanaverageincreaseof2758mgm-2 inBlack-eyed pea and of 1612 mg m-2 in Grey-eyed pea. Broccoli crop inrotation with cowpea showed the same pattern with regard to CH4

emissions, with an increase of 529 mg m-2 in broccoli rotated withBlack-eyedpeaandof1293mgm-2inrotatedwithGrey-eyedpea.Weobserved significant negative correlations between CH4 and TN, β-glucosidase and β-glucosaminidase. Organicmanagement contributedtoreducingCH4fluxes,andpromotedanincreaseinsoilTNcontentandβ-glucosidase.

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Session 18, parallel: Resistance to biotic and abioticstresses-RoomArrábidaIVChaired by Weidong Chen (USDA-ARS, USA) and LaurentGentzbittel(CNRS,France)

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OralCommunications16:30-16:40 Oral–S18ExploringmetabolicchangesinlegumesexposedtocombinedbioticandabioticstressDickinsonM.1,RusilowiczM.2,WilsonJ.2,CharltonA.11-FeraScienceLtd,NationalAgrifoodInnovationCampus,SandHutton,YorkYO411LZ,UK2-YorkCentreforComplexSystemsAnalysis,UniversityofYork,YorkYO105GE,UKTheyieldoflegumecropsisseverelyunderminedbybothdroughtandfungalinfection.AcurrentEUFP7-project(ABSTRESS)isusingstate-of-the-artplantbreedingtoolsalongsidethestudyofgeneandmetaboliteexpressiondatainlegumespeciestostudytheirresistancemechanismsto combined stresses.Metabolomicsdata canbeusedasmarkers forthe expression of crucial hub genes associatedwith stress resistance,significantlyreducingthetimetakentodevelopnewcropvarietiesabletowithstandthechallengesassociatedwithclimatechange.This presentation will discuss metabolomics data obtained from themodellegumeMedicagotruncatulaexposedtocombineddroughtandFusarium infection over 12 days. Metabolite profiles were obtainedfromboth leaves and roots for every day over the 12 days andwereacquired using liquid chromatography-high resolution massspectrometry (LC-HRMS), with further metaboliteannotation/identification by LC-HRMS/MSn. Biochemical changesobserved in lipid, flavanoidandalkaloidmetabolism inplantsexposedtobothstresseswillbediscussed.To support findings, a second metabolomics experiment wasundertakeninpeausinganidenticalsetupasthemodel.Thedatafromthis confirmatory studywill also be discussed, highlighting changes inmetabolites and subsequent pathways common to both sets of data,with particular focus on legumes exposed to combined stresses overtime.

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16:40-16:50 Oral–S18TheroleofplantcellwallinresistanceandsusceptibilitytopathogenicpathogenToyodaK.1,MikiM.1,YamasakiS.1,YaoS.1,MatsuiH.1,NoutoshiY.1,YamamotoM.1,IchinoseY.1,ShiraishiT.1,21-OkayamaUniversity,Japan2-ResearchInstituteofBiologicalSciences,Okayama,JapanSuccessful colonization by an appropriate pathogen can bethereforeachievedbythesuppressionorcircumventionofPAMP-triggered immunity through the secretion of pathogen-derivedeffectors. In Mycosphaerella pinodes, which causes leaf spots(blights)ofpea,twostructurallyrelatedglycopeptidesuppressorsnamed supprescins A and B are secreted in the pycnosporegermination fluid. Pure suppressors potently inhibit the ecto-ATPase (apyrase; EC3.6.1.15) of host cell wall, temporarilyreducing the ability of the host cells to defend themself. Thecatalytic activity in extracts from the cell walls of pea wasenhanced in vitro, with the consequent increase of ROSgeneration by extracellular peroxidase(s), when exposed to thefungalelicitor.Interestingly,theBlue-NativePAGEanalysisforcellwall proteins from pea epicotyls demonstrated that cell wall-associated, ecto- ATPase(s) formed a large protein complex(es)ranging from 450 to 900 kDa, one of components was thehydrogen peroxide-producing copper amine oxidase (CuAO). TheCuAO activity was coordinately regulated with ATP-hydrolyzingactivity invitro,byanelicitorandasuppressor fromM.pinodes.Moreover, in vitro treatment of cell wall proteins with thesuppressor caused an appearance of the apyrasemonomer. It isthus likely that M. pinodes targets the host ecto-ATPase-containing protein complex(es) to attenuate cell wall-based,extracellulardefense(s).

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16:50-17:00Oral–S18DetectingtolerantgermplasmandQTLsassociatedwithfloodingstressindrybean(PhaseolusvulgarisL.)SoltaniA.1,MoghaddamS.M.1,WalterK.1,Restrepo-MontoyaD.1,SchroderS.1,MamidiS.2,LeeR.1,McCleanP.1,OsornoJ.M.11-NorthDakotaStateUniversity,DepartmentofPlantSciences,Fargo,ND,USA2-HudsonAlphaInstitute,Huntsville,AL,USAFlooding is one of the most devastating abiotic stress, endangeringfood security. This stress can drastically modify several physiologicalpathways of plants, which will eventually affect seed yield. Eventhough dry bean is highly susceptible to flooding, there are a feweffortsaddressing this issue.To identify thepotentialmechanismsbywhich dry bean tolerate the flooding stress, we evaluated ~500genotypes under greenhouse conditions at two growth stages:germination and seedling. Seven traits were evaluated and analyzedandusedforaGenome-wideAssociationstudy(GWAS)wasperformedusing ~150K SNPs. In general, results suggest that middle-Americangene pool is using different physiological mechanisms than Andeangene pool to deal with waterlogging stress. Within each pool,significant differenceswere also detected amongmarket classes. Forinstance, chlorophyll content was well preserved in pinto and greatnorthernmarketclasses(16%reduction).Howeverblackandsmallredmarket classes weremore tolerant to flooding in germination stage.The resultsofGWAS indicate that tolerant towaterloggingstress isapolygenetic trait controlled bymore than 30 loci. Among them, twoQTL on Pv08/1.6 and Pv02/41.1, which control root weight andgerminationrateunderfloodingconditionswerealsoreportedtoplaysimilar role in soybean. Identifying tolerant genotypesalongwith theassociatedQTLwillsetthestageforimprovingwaterloggingtoleranceindrybean.

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17:00-17:10Oral–S18Genome-WideassociationmappingoffrosttoleranceinPisumsativumBejiS.1,FontaineV.2,DevauxR.3,NegroS.5,6,BahrmanN.2,AubertG.4,DelbreilB.1,Lejeune-HénautI.21-USCICV-AFP,UniversitéLille1,59655,Villeneuved'Ascq,France2-USCICV-AFP,INRA,80203,Péronne,France3-UEGCIE,INRA,80203,Péronne,France4-UMRAgroécologie,INRA,21065,Dijon,France5-UMRGQE,INRA,91190,GifsurYvette,France6-UMRMIA-Paris,AgroParisTech,75005,Paris,FranceGenomeWideAssociationMappingwas performed in pea. Accessionsfrom the pea reference collectionwhere phenotyped for frost in fieldandcontrolledconditions,andgenotypedusingInfinium®BeadChip15KSNPs (Tayeh et al., 2015). After applying filters of quality control, weobtained 363 accessions and 10739 loci for GWA study. AssociationanalyseswereconductedwithFaST-LMMsoftwareusingamixedmodelthatincludedarelatednesskinshipmatrix(K)andapopulationstructurematrix (Q) to control for false positives. The K matrix was generatedusingtwoapproaches.Inthefirstone,thekinshipmatrixwasestimatedwithall the10739markers. In thesecondapproach,weestimatedthekinship,called“K-chr”,withallthemarkersotherthanthoselocatedonthesamechromosomeasthemarkerbeingtested(Rincentetal.,2014).Simulations revealed thatRincent’s approachwasmorepowerful thanthemixedmodeltakingintoaccountageneralkinship(estimatedforallchromosomes). The GWA study identified 8 loci distributed overdifferentchromosomescomprising61SNPssignificantlyassociatedwithfrosttolerance.Resultsconfirmed3QTLsthatwerepreviouslymappedusing bi-parental populations and identified 3 novel tolerance loci.Several potential-candidate genes were found corresponding to theseSNPs. Additionally, the analyses allowed to identify haplotypes withincreasedfrosttoleranceandaccessionswithfavourableallelesforthistrait.

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17:10-17:20 Oral–S18Marker-assistedbreedingstrategiesforanthracnoseresistanceinlupinRuge-WehlingB.1,FischerK.1,DieterichR.2,RotterB.3,WinterP.3,WehlingP.11 - Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute forBreedingResearchonAgriculturalCrops,GroßLüsewitz,Germany.2-SaatzuchtSteinachGmbH&Co.KG,Bocksee,Germany.3-GenXProGmbH,Frankfurt/Main,Germany.Anthracnose, causedby the fungusColletotrichum lupini, representsthemost importantdiseasein lupincultivationworldwide.Toobtainhigh and stable yields it is necessary to plant resistant cultivars astherearenopossibilitiestoovercomethefungusviapesticides.Setsof plant genetic resources of sweet narrow-leafed and yellow lupinwere assessed in their susceptibility to anthracnose. In both lupinspecies breeding lines were identified which displayed a qualitativeresistance,eachgovernedbyadominantgeneasrevealedbygeneticanalyses of segregating F2 families and their F3 progenies. Multi-annual field-tests with the novel resistant resources of blue andyellow lupins confirmed its high and stable level of anthracnoseresistance. The underlying genes were designated LanrBo (L.angustifolius) and Llur (L. luteus). Different resourceswere used fordevelopingmolecularmarkers for the resistance, namely, (I) anchormarkersalreadymappedinL.angustifolius,(II)sequenceinformationdrawn from model genomes and (III) sequence information fromdifferentiallyexpressedcDNAderivedfromRNA-Seq.Linkageanalysisallowed us to locate LanrBo on linkage group NLL-11 of Lupinusangustifolius.Twoflankingmarkersresultedinamarkerbracketwithajointrecombinationoflessthan0.2%.Thetwomarkersarealreadyintegrated in breeding programs. Highly polymorphic SNPs areavailableforLlurandarecurrentlyusedformapping.

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17:20-17:30ORAL–S18UseofwildrelativesinpeabreedingfordiseaseresistanceRubialesD.1,BarilliE.1,RispailN.1,CastillejoM.A.1,Aznar-FernándezT.1,FondevillaS.11 - InstitutodeAgriculturaSostenible,CSIC,Avda.MenendezPidal s/n14004,Cordoba,SpainPea(Pisumsativumssp.sativum)isacoolseasonlegumecropproducedworldwide, mainly in temperate regions whose seeds can be used tofeed livestock or for human food. Pea yield can be constrained by anumber of pests and diseases to which there is insufficient geneticresistance available. Wild relatives, including related subspecies of P.sativum,oreventhemostdistantP.fulvumoffersareservoirofsourcesofresistancethatcanbeexploitedinpeabreeding.At CSIC at Córdoba we have screened large germplasm collections ofPisum lookingforresistancetoascochytablight,powderymildew,rust,broomrape, fusarium wilt, aphid and weevil. This resulted in theidentificationofvaluablesourcesof resistancethatwere introduced inour breeding program. As an example, a new gene (Er3) for powderymildew resistance was identified in P. fulvum and introduced in pea,with a resistant cultivar (‘Eritreo’) already protected in Europeancatalogue.Similarly,thefirsttwobroomraperesistantcultivarsarenowprotected,andanumberofbreedinglinesarebeingdeveloped.Parallelto thisappliedapproach, someof the identified resistanceshavebeencharacterizedatthehistochemist,geneticandproteomiclevel.

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PostersP140–S18 Er3gene,conferringresistancetoErysiphepisi,islocatedinpeaLGIV.CobosM.J.1,RubialesD.1,FondevillaS.11 - Institute for Sustainable Agriculture-CSIC, Avda. Menendez Pidal s/n, 14004,Cordoba,SpainThreegenesforresistancetoErysiphepisi,nameder1,er2andEr3havebeendescribedinpeasofar.er1geneislocatedinpealinkagegroupVI,whileer2genehasbeenmappedinLGIII.SCARandRAPDmarkers tightly linked to Er3 gene have been identified, but thepositionofthesemarkers inthepeageneticmapwasunknown.Inthis study the pea cv.Messire and a near isogenic line ofMessirecontaining Er3 gene (cv. Eritreo) were surveyed with SSRs withknown position in the pea map. Two SSRs previously mapped inLGIV were polymorphic between cv. Messire and cv. Eritreo andbetween two contrasting bulks homozygous forEr3 or er3 alleles.Further linkage analysis confirmed that these SSRswere linked toEr3 and to theRAPDand SCARmarkers previously reported to belinked to this gene. Therefore, this study demonstrates that Er3geneislocatedinpeaLGIV.

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P141–S18 DevelopingDroughtandHeatStressTolerantChickpea(CicerarietinumL.)GenotypesYücelD.,AngınN.,MartD.,TürkeriM.,ÇatalkayaV.,YücelC.EasternMediterraneanResearchInstitute,Adana,Turkey.Chickpea(CicerarietinumL.)withhighproteincontentisavitalfood,especially in under-developed and developing countries for thepeoplewhodonotconsumeenoughmeatdue to low-income level.The objective of the proposed study to evaluate growing, yield andyield components of chickpea genotypes under Mediterraneancondition so determine tolerance of chickpea genotypes againstdrought and heat stress. For this purpose, a total of 34 chickpeagenotypes were used as material. The experiment were conductedaccordingtofactorialrandomizedcompleteblockdesignwith3repsat theEasternMediterraneanResearch Institute,Adana,TURKEY for2014-15 growing season under three different growing conditions(Wintersowing,irrigated-latesowingandnon-irrigated-latesowing).According to resultsof thisexperiment, vegetativeperiod, floweringtime, poding time, maturity time, plant height, height of first pod,seedyieldand100seedweightwererangedbetween68.33to78.77days, 94.22 to 85.00 days, 94.11 to 106.44 days, 198.56 to 214.44days, 37.18 to 64.89 cm, 18.33 to 34.83 cm, 417.1 to 1746.4 kg/haand 14.02 to 45.02 g, respectively. Among the chickpea genotypes,Aksu,Arda,Çakır, F409 (X05TH21-16189), FLIP03-108were leastaffectedbydroughtandheatstress.Therefore,thesegenotypescanbeusedassourcesofdroughtandheattoleranceinfurtherbreedingprogrammeforevolving thedroughtandheat tolerantgenotypes inchickpea.

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P142–S18 Starterfertilisers:Dotheyinfluencerhizobialpopulationsinviningpeafields?WieselL.1,MaguireK.2,WardR.L.1,FrancisS.31 - Processors and Growers Research Organisation, Great North Road, Thornhaugh,Peterborough,PE86HJ,UK2-FormerlyatPGRO,nowatBayerCropScienceLimited,230CambridgeSciencePark,Cambridge,CB40WB,UK3-FenPeasLtd,TheOldFarmHouse,OldLeake,Boston,PE229HR,UKPea yields have reached a plateau inmany areas over recent yearsand one option to increase yields is the application of starterfertilisers.Starterfertiliserscontainphosphorus,whichpromotesrootgrowth and is essential for root nodulation to occur. Some starterfertilisers,however,containnitrogenwhichispotentiallydamagingtorhizobial populations thereby not only reducing nitrogen fixation inthepea cropbut also leaving reduced levelsof soil nitrogen for thesubsequent crop. The impact of starter fertilisers with and withoutnitrogen on vining pea yields and rhizobial populations in soils hasbeeninvestigatedintwosubsequentyears.Threefieldtrialsinearly,midand late-drilledcropswereestablishedeachyear.Yields tendedto increase in mid and late-drilled crops and preliminary resultssuggest that the application of starter fertilisers did not affectrhizobial populations in soils. Application of starter fertilisers is notverycostlytogrowersandanypeayield increaseofgreaterthan73kg/hawill result inaneconomicbenefit forpeagrowers. If itcanbeshown that starter fertilisers do not negatively impact rhizobialpopulationstheuseofstarterfertilisersholdsgreatpotentialfortheviningpeaindustry.

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P144–S18 RNA-seqanalysisofgeneexpressioninwildandcultivatedlentilsinresponsetoAscochytalentisinfectionGarcíaP.,SáenzdeMieraL.E.,VencesF.J.,VaqueroF.,GonzálezA.I.,PolancoC.,PérezdelaVega,M.ÁreadeGenética,Fac.Biología,UniversidaddeLeón,24071León,SpainLentilascochytablightiscausedbythefungalpathogenA.lentisandisan important disease inmost lentil producing countries. Ascochyta isfavoured by cool andwet conditions and can reduce crop yields, butalso affects seed quality and hence theirmarket value. Resistance toascochytablighthavebeen identifiedboth incultivatedandwildLensspecies.Weused the susceptible L. c. culinaris cultivar ‘Alpo’ and theresistant wild L. odemensis ILWL235 to investigate the differences ingeneexpressionafterinfection.Total RNA was isolated from the aerial parts of infected and non-infected 15-day-old plantlets that were collected 24 hours after thetreatment.RNA-seqwasperformedusingthreebiologicalreplicatespereach species and treatment on an Illumina HiSeq 2500. This yieldedover 435million 100nucleotide paired-end reads,with an averageof18 million read-pairs per replicate. Alpo sequences were aligned tonon-annotated lentil genome v0.8 using STAR tool and theirtranscriptome was assembled with TRINITY. Read counts for eachreplicate were then assessed for differential expression using the RpackageDESeqwithRSEMmappingtool.We found that880genes increased theirexpressionafter infection inboth specieswhile 23 decreased it. In the resistant L. odemensis theincrease in 256 genes and the decrease in 207 geneswere detected,while 1810 and 1053 genes showed, respectively, expression changesonlyinthesusceptiblecultivar.

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P145–S18 CharacterizationofMildewresistancelocus(Mlo)genesinwildspeciesoflentilCanoJ.,Fernández-TúñezC.,SáenzdeMieraL.E.,PérezdelaVegaM.,PolancoC.ÁreadeGenética,Fac.Biología,UniversidaddeLeón,24071León,SpainPowdery mildew is a widespread disease that can cause significantharvest losses in cropplants in temperate climates. Loss-of-functionmutantallelesofspecificMLOgenesconferbroad-spectrumpowderymildew resistance in monocots (barley) and dicots (Arabidopsisthaliana, tomato, and pea). SNARE genes are required for mlo-specifiedresistance.MLOgenesarepresentassmallfamiliesinplantsand we previously identified 14 MLO gene family members in thelentilgenome(Lensculinarisculinariscv.CDC-Redberry)bysequencesimilarity-based homolog searches using the available sequences inpeaandsoybean.The phylogenetic analysis revealed that three genes (LcMLO_01,LcMLO_02, LcMLO_10) were homologous of MLO genes associatedwith powdery mildew resistance in other species. A set of primerswas designed in order to amplify the complete LcMLO_01 genesequencefrominteresting lentilaccessionsandwildrelativespecies.HerewepresentSNAREgenecandidates in lentilandthesequencesof LcMLO_01 in L. c. orientalis, L. odemensis, L. tomentosus, L.nigricans, and L. lamotei. Most of the nucleotide differences weredetectedinthe14intronicregionsbutaminoacidchangeswerealsoidentified. The deduced protein sequences showed the presence ofcharacteristic and conserved transmembrane motives and aconserved calmodulin-binding domain. A phylogenetic tree wasconstructed using the corresponding nucleotide sequences of thisgeneincludingcultivatedlentillines.

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P146–S18 Peastraw:anadvantageousco-productindairygoatdietsColladoE.1,FernándezJ.A.2,VivancosN.3,MoyaF.4,ArmeroE.5.1-InstitutodeBiotecnologíaVegetal2-DepartamentodeProducciónVegetal3-DepartamentodeCienciayTecnologíaAgraria4-KapraSociedadCooperativa,5DepartamentodeCienciayTecnologíaAgrariaLegume production offers us different co-products to use in animalnutrition.Thenutritivevalueofthelegumestrawishigherthancerealstraw with a slightly higher percentage of crude protein, higherdigestibility,andlowerpercentageofneutraldetergentfibre.Theaimofthisworkwastoreplacecerealstrawbypeastrawandtoanalyseitseffectonmilkyieldandmilkqualityindairygoat.Theexperimentwas carriedout during July of 2015 in a commercial farm inMurcia(Spain).200goatswerefedwithcerealstraw(cerealstrawgrouporCSG) and in thediet of 200 goats cereal strawwas replacedbypeastraw (pea straw group or PSG). Milk yield and its quality wereassessedwithoverallinformationfromeachgroupalongthetrialandwith individual data nine days after the diet was modified. Feedintakewasmeasured through overall information from each group.Considering overall measurements, PSG had a slightly higher milkyield, with a higher percentage of fat, and higher food intake thanCSG. However when the results were analysed with individual datathere was a trend to produce more milk in PSG, but it was notstatistically significant, and no differences were observed in milkquality.Therefore,theuseofpeastrawindietsfordairygoatscouldenhance farm sustainability without affecting milk production, butpossiblemodificationsinmilkquality,suchasfattyacidoraminoacidcompositions,shouldbeassessedinfutureresearches.

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P147–S18 Genome-wideassociationstudyofcommonbeandiseaseresistanceinPortuguesegermplasm.LeitãoS.T.1,AlvesM.L.1,RubialesD.2,VazPattoM.C.11 - InstitutodeTecnologiaQuímicaeBiológica,UniversidadeNovade Lisboa,Oeiras,Portugal2-InstituteforSustainableAgriculture,CSIC,Cordoba,Spain.Commonbean (Phaseolus vulgaris L.) is theworld’smost importantgrain legume for human consumption. Portugal holds a verypromising common bean germplasm,which results frommore thanfivecenturiesofnaturaladaptationandfarmer’smassselectioninthecountry, not yet fully explored in breeding. Fusariumwilt (Fusariumoxysporum f. sp. phaseoli) is among the diseases that cause majoryieldlossesinthiscrop.Inordertoidentifynewsourcesofresistanceand to unravel the associated genetic control we characterized theresistancetofusariumwiltofacollectionof150Portuguesecommonbean accessions. Evaluation took place under growth chamberconditions, using a replicated complete randomized block design.Infection responses revealed great variability among the accessionswith the identification of interesting sources of resistance. Thiscollectionwasadditionally screenedwith~12000molecularmarkers(SSRandSNPsfromIlluminaBeadChipandDArTseqarrays)uniformlydistributed throughout the genome. A genome-wide associationstudy, joining the disease resistance levels with the genomicinformation, is currently ongoing, using a mixed linear modelapproach accounting for population structure and familialrelatedness. The putative statistically significant associations foundwillcontributetoamoreeffectiveselectionofcommonbeangeneticresourcesinfuturediseaseresistancebreedingprograms.

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P148–S18 Nitrogenandphosphorususeefficiencyintherecombinantafilalinesofamappingpopulationoffieldpea(PisumsativumL.)GawłowskaM.1,GórnyA.1,RatajczakD.1,NiewiadomskaA.2,ŚwięcickiW.1,BeczekK.1,KnopkiewiczM.31-InstituteofPlantGeneticsPolishAcademyofSciences,Poznań,Poland,2-UniversityofLifeSciences,Poznań,Poland,3-AdamMickiewiczUniversityinPoznańIn European countries productivity of field pea is considerablyaffected by instabilities in water and nutrient accessibility. In theseconditions, yield potential depends on plant physiological capacitiesto an efficient utilization of environmental resources (water,nutrients).In fieldexperiments, varianceandco-variancebetweencomponentsofnitrogenandphosphorusefficiencywasevaluatedamong linesofthe Canadian mapping population [Carneval_MP1401] during thewholegrowthseasonundervariednitrogennutrition.The pea yield and nitrogen utiliztion efficiency (NERgen) werepositively correlated in optimal and stress localizations (Wiatrowo,optimal conditions r=0.95, stress conditions: Wiatrowo r=0.50,Przeb_dowo r=0.65). The seed yield and phosphorus utilizationefficiency (PERgen) were positively correlated only in optimalcondition(Wiatrowo,r=0.58).Soiltreatmenteffects(E)weresignificantforallcharacters,althoughgenotype-treatment (G-E) interactions were significant only fornitrogen content in seeds (%Ngen). Noteworthy, relationshipsbetweenGYandnitrogenefficiencywerestrongerinnitrogen-limitedconditions(optimalconditionsr=0.31,stressconditions:Wi,r=0.50,P,r=0.65).The results indicate that the importance of components ofphysiological efficiency for pea yielding increases in the sub-optimalconditions. Broad variability of all physiological traits gives a highprobabilityofQTLmappingwithsuccess.

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P149–S18 UnderstandingthemolecularmechanismsunderlyingthedifferentialresponsetowaterdeficitofMedicago

truncatulaexpressingAtTPS1geneBrancoD.1,Almeida,A.M.2,AlcântaraA.3,4,SilvaJ.M.3,4,SilvaA.B.3,4,CoelhoA.5,FevereiroP.1,4,AraújoS.1,61-PlantCellBiotechnologyLaboratory,ITQB-NOVA.saraujo@itqb.unl.pt2 -RossUniversity, SchoolofVeterinaryMedicine,P.O.Box334,Basseterre,SaintKittsandNevis3 - Biosystems and Integrative Sciences Institute (BioISI), Faculdade de Ciências,UniversidadedeLisboa,CampoGrande,1749-016Lisbon,Portugal.4 - Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa,CampoGrande,1749-016Lisbon,Portugal.5 - Proteomics ofNon-ModelOrganisms Laboratory, Instituto de TecnologiaQuímica eBiológica António Xavier (ITQB), Universidade Nova de Lisboa (UNL), 2780-157 Oeiras,Portugal.6 - Plant Biotechnology Laboratory, Department of Biology and Biotechnology 'L.Spallanzani',UniversitàdegliStudidiPavia,viaFerrata1,27100Pavia,Italy.Themanipulationof trehalosemetabolism isapromisingapproach toimprove abiotic stress tolerance in plants. We have shown thatMedicago truncatula plants engineered with trehalose-6-phosphatesynthase1(AtTPS1)fromArabidopsishaveimprovedresponsetowaterdeficit(WD).This work aims to unveil the molecular mechanisms underlying thedifferential response of AtTPS1 expressing plants. The transcriptomeand proteome changeswere studied in roots and leaves ofwild type(M9-10a) and transgenic (TPS+) plants grown under well watered(WW),WDandWDrecovery(WDR)conditions.Differentially expressed transcripts encoding for transcription factors(TFs) were extracted from Illumina-based MACE data. Hierarchicalclustering evidenced TFs with contrasting expression profile betweenlinesthatmaylikelybeinvolvedintheimprovedWDresponseofTPS+plants.Differentially accumulated proteins (DAPs) were identified from 2D-DIGE coupled to MALDI TOF/TOF analysis. The number of DAPsbelonging to each MapMan functional category depends on the line/organ studied. Hierarchical clustering revealed thatM910a and TPS+have different protein accumulation profiles upon WD and WDR inresponsetoappliedtreatments.Theintegrationofphysiological,proteomicandtranscriptomicdatawillenhance our knowledge on metabolic pathways activated with themanipulationof trehalosemetabolism.Thisknowledgecanbeused inthedesignandselectionoflegumeswithimprovedWDresponse.

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P150–S18 GeneticandgenomicmappingofgenesconferringresistancetofouranthracnoseracesincommonbeangenotypeBAT93MurubeE.,CampaA.,Ferreira,J.J.Plantgeneticgroup,SERIDA,Asturias,SpainAnthracnose, caused by the fungus Colletotrichum lindemuthianum(Sacc.&Magnus)Lamb.-Scrib.isoneofthemostdestructivediseasesofcommonbean(PhaseolusvulgarisL.).Resistancetoanthracnoseincommonbeangenerally followsaqualitativeandrace-specificmodeof inheritance. In this work, using forward genetic analysis, weinvestigate the physical location in the bean genome of genesconferringresistancetoanthracnoseraces6,38,39and357inaRILpopulation (140 lines) derived from the cross Xana × BAT93(Susceptible x Resistant). Resistance to four races fitted to thatexpected for one resistance gene. Co-segregation in pairs wasobserved between response to races 6 – 357 and 38 – 39. Onerecombinant line resistant for races 38 and 39 and susceptible forraces 6 and 357 was observed suggesting that two closely linkedgenesareinvolvedintheresistance.Adensegeneticlinkagemapwasdeveloped in thisRIL population throughGenotypingby Sequencing(GbS). A total of 7156 SNPswere obtained although 1566were notconsidered due to its distorted segregation. Resistance genes weremapped on linkage group Pv04 between markers SNP04_031(physical position 1.61 Mb) and SSR4_1.743.4 (1.74 Mb). In silicoexploration in the bean reference genome revealed 8 genes thatcodifyproteinswithLRRdomains,normally involved intheresponsetopathogensthusbeingcandidatestobecontrollingthisresistance.

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P151–S18 Geneticresistancetopowderymildewincommonbean:recentadvances.FerreiraJ.J.,MurubeE.,CampaA.PlantGeneticGroup,SERIDA,Asturias,SpainPowdery mildew (PM) is a serious disease in many legume speciesincluding common bean (Phaseolus vulgaris L.). In this work, thespeciescausingPMinthebeanlocalcropswasidentifiedasErysiphepolygonithroughmolecularanalysisoftheinternaltranscribedspacerregion.Undercontrolledconditionsinseedlingtests,16of288testedmaterials showed a complete resistance without visible symptoms.Inheritance of resistance was studied in the Xana/Cornell RILpopulation, and in five F2:3 segregating populations involving theresistance sources G122, Porrillo Sintético, BGE003161, Belneb andG19833. Results revealed a qualitative mode of inheritance for PMresistancebeingidentifiedtwoindependentresistancegeneslocatedonlinkagegroups(LG)Pv04andPv11.Thesegenesshowadominantepistatic relationship, the gene conferring total resistance responsemaskingtheeffectofthegeneconferringpartialresistance.ThegenegoverningtotalresistancewasmappedonLGPv04incultivarsPorrilloSintetico, BGE003161, and Belneb, while in Cornell 49242 wasmapped on LG Pv11. The gene conferring partial resistance wasmappedonLGsPv11andPv04incultivarsX2776andCornell49242,respectively. Interestingly, both resistance genes were mapped inregions with many genes codifying to typical R proteins. Thisinformation will be relevant for the implementation of breedingprograms focused on the development of cultivars carrying geneticresistancetoPM.

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P152–S18 ThepursuitforresistancesourcestobioticstressesinLathyrussativusAlmeidaN.F.1,GonçalvesL.1,LourençoM.2,JuliãoN.3,Aznar-FernándezT.4,RubialesD.4,VazPattoM.C.11 - InstitutodeTecnologiaQuímicaeBiológicaAntónioXavier,UniversidadeNovadeLisboa,Av.daRepública,2780-157Oeiras,Portugal.2-CâmaraMunicipaldeAlvaiázere,PraçadoMunicípio,3250-100Alvaiázere,Portugal.3Simões&RamosLda,EstradaNacional110,3250-404RegodaMurta,Portugal.4-InstituteforSustainableAgriculture,CSIC,Apdo.4084,E-14080Córdoba,Spain.Theneed formore sustainable andenvironmentally safe agriculturehasreinforcedinterestinlegumecultivationinEurope.Thesespecieshavetheuniquecapacityforsymbioticnitrogenfixation,highlightingtheir importance in both natural and agricultural ecosystems. Inaddition, genotypes that are naturally resistant to pathogens areusefulforreducingpesticideapplication.Grasspea(Lathyrussativus)isafeed,foodandfoddercrop,withgreatadaptabilitytoinauspiciousenvironments, high protein content and resistance to seriousdiseases. It is one of the most promising sources of calories andproteinforthedrought-proneandmarginalareasofAsiaandAfrica,being an alternative to marginal soils and for cropping systemsdiversificationinEurope.As part of the European LEGATO and the Portuguese QUALATYprojects,wefocusonthesearchforresistancesourcestoweevilsandfungaldiseasesonacollectionofhundredgrasspeagenotypesfromdiverse origins, including Europe, Africa and Asia. Along with thephenotypic observations (resistance level) on field and controlledconditions, we used DArT-seq and E-SSRs to access the geneticdiversity within and among grass pea accessions. This will allowidentifyinganyrelationshipbetweengeneticdiversityandresistancelevel. The acquired data will reveal valuable diverse sources ofresistance for the development of modern grass pea cultivars,improvingtheattractivenessofthisunderusedbutpromisingcrop.

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P153–S18 VariabilityofN2fixationincowpeaunderphosphorusdeficiencyisrelatedtophosphorususeefficiency.LazaliM.1,3,BrahimiS.1,OunaneS.M.2,DrevonJ.J.31 - Département d’Agronomie, Faculté des sciences de la nature et de la vie et dessciencesdelaterre.UniversitéDjilaliBounaamaKhemisMiliana.RouteThnietElHad,Soufay44225KhemisMiliana,Algérie.2 -Département de ProductionsVégétales. EcoleNationale SupérieureAgronomique(ENSA).AvenueHassanBadi16200Alger,Algérie.3 - Institut National de la Recherche Agronomique, UMR Ecologie Fonctionnelle &Biogéochimie des Sols et Agroécosystèmes, INRA-IRD-CIRAD-SupAgro. Place PierreViala,34060Montpellier,France.Low availability of phosphorus (P) is a major constraint to legumeproduction,andeffortsarebeingmadetoidentifylegumegenotypeswith tolerance to low P and greater P use efficiency (PUE). Cowpea(Vigna unguiculata L.) genotypes vary in their adaptation to low-Psoils. In order to investigate to what extent this variation may berelated to PUE for symbiotic nitrogen fixation (SNF), five genotypes,Adrar, El Kala, Djenet, Tizi Ouzou and Bejaia, were grown in semi-hydroponic culture with sufficient versus deficient P supply. At theflowering stage, the biomass of plants and nodules and their Pcontents was determined after measuring the quantity of N2 fixed.TheresultsshowedthatnoduleandshootbiomasswerelesswhenPwas deficient. The genotypes that presented the maximum growthduring the experiment presented a high efficiency in use of therhizobialsymbiosiscalculatedastheslopeofplantbiomassregressionasafunctionofnodulation.UnderP-deficiency,efficiencyinuseofPfor SNFwas significantly increased in the genotypes El Kala, DjenetandAdrarandaccompaniedwithanincreaseoftheefficiencyinuseof the rhizobial symbiosis. Hence the large differences show thattraits for more P uptake-efficient plants exist in the tested cowpeagenotypes. This opens the possibility to breed for more P uptake-efficientvarietiesasawaytobringmoresparinglysolublesoilPintocyclingincropproductionandobtaincapitalisationofsoilPreserves.

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P154–S18 SoilmicrobialcommunitydiversityincreasesresilienceofpeaplantafterdroughtSorinC.,MaronP.A.,GirodetS.,NowakV.,DucG.,PrudentM.Institut Nacional de la recherche agronomique, INRA, UMR1347 Agroécologie, F-21065Dijoncedex,FranceWe hypothesized that the stability (resistance and resilience) of peaproduction under drought may be enhanced with increasing thediversityofsoilmicrobialcommunities.Anexperimentwasconductedunder greenhouse conditions where pea plants were grown duringthreemonthsunderthreelevelsofsoilmicrobialcommunitydiversity,obtainedbyadilutionapproach. Forhalfof them,awater stresswasappliedduringtwoweeksatvegetativestagefollowedbyarewateringuntil the end of the growth cycle, while the other plants were wellwatered throughout the experiment. We measured traits reflectingplant development and growth, plant resource acquisition andallocationamongcompartmentsandseedproduction(i)afterdroughttoevaluatetheresistanceoftheplant,and(ii)atphysiologicalmaturitytoevaluatetheresilienceoftheplant.Ourresultsshowthattheplantbiomass depletion due to drought did not vary with the level ofmicrobial diversity. Adaptation of root system and plant transpirationwasdependentonthe levelofmicrobialdiversity:under lowdiversitylevel, root traits and stomatal conductance were less affected bydrought than under high diversity level. After rewatering, plants ininteractionwithahigherdiversitylevelhadahigherbiomass,indicatinga better resilience. Seedproductionwas also enhancedby thehighermicrobialdiversitylevel.

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P155–S18 Marker-assistedBackcrossSelectionofVirusResistanceinPea(PisumsativumL)SceguraA.,McPheeK.NorthDakotaStateUniversity,USAVirusesareadestructiveplantpathogenresulting insignificantyieldlossandreducedgrainquality.Peaseed-bornemosaicvirus(PSbMV)is an economically important viral disease in pea (Pisum sativum L.)and has recently been detected in the Northern Great Plains withsignificantimpactontheindustry. PSbMVisaphid-transmittedfromplant to plant and can be seed-borne. It causes malformed leaves,discoloredorsplitseedandreducedsizeandnumberofseed.Markerassisted backcross breeding will be used to transfer the singlerecessive resistance allele for PSbMV (sbm-1) located on LG VI intolocally adapted breeding lines. Parental breeding lines werehybridizedwithcv.Lifterinthefallof2015todevelopF1progeny.F1plantswereselfedorbackcrossedtotheadaptedbreedinglineastherecurrentparentinthegreenhouseinthespringof2016.F2andBC1seedwillbeplantedinthefieldandindividualplantspossessingsbm-1 identified using the 4Egenomic primers previously developed.Individualplantscontainingsbm-1willbeallowedtoself-pollinatetoproduceBC1F2 seedorwill bebackcrossed to the recurrentparent.F5, BC1F4 and BC2F3 populations will be evaluated for diseaseresistance against the PSbMV strain present in the Northern GreatPlainsandforagronomicadaptationincludingyieldperformance.

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P156–S18 Atranscriptomicapproachidentifiescandidategenesfordroughttoleranceduringthereproductivephaseinpea.VernoudV.1,RossinN.1,PrudentM.1,LeSignorC.1,Sanchez,M.1,PateyronS.2,BalzerguesS.2,AubertG.1,BurstinJ.1,GallardoK.1,ThompsonR.1.1-Agroécologie,AgroSupDijon,CNRS,INRA,Univ.BourgogneFranche-Comté,F-21000Dijon,France.2-POPSTranscriptomicPlatform,IPS2,CNRS,INRA,Univ12ParisSud,UnivEvry,UnivParis-Diderot,SorbonneParis-Cite,UnivParis-Saclay,91405Orsay,FranceDrought is amajor environmental factor limiting the productivity ofcropplants. Inpeadrought stressoccurringduring the reproductivephase cangreatly affect seed yield andquality.We investigated theresponseofpeaplants(var.Caméor)subjectedtowaterstressduringtheseedfillingperiod,aphasewhenmassiveremobilizationfromthevegetativeorgansoccurstosustainseedhigh-nitrogendemand.Peaplantsweresubjectedtodroughtstressatthebeginningoftheseed-filling period of the first two nodes for 8 days. Total and one-seedbiomassdecreasedby35%and20%respectivelybythislimitedwaterstress. Nitrogen allocation to the different plant compartmentswasalso affected,with an increasedN allocation to the leaves from thevegetativenodesandadecreasetotherootandseedcompartments.Transcriptomicchanges inwater-stressed leaves fromthevegetativenodeswereanalysedbyhybridizationofa40kpeamicro-array:178geneswereat leasttwofoldup-regulated inwater-stressedsamplescompared to well-watered samples, whereas 55 genes were down-regulated.Among themost strongly up-regulated geneswere thoseencoding a glutamine amidotransferase, a sucrose transporter fromthe SWEET family, a phosphoenolpyruvate carboxykinase and acarotenoid cleavage dioxygenase involved in strigolactonebiosynthesis.Acknowledgement: this project was supported by the BurgundyRegion(AGRALE6,FABERprogram)andtheFP7LEGATOproject.

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P157–S18 Theuseofmicrosatellitesmarkersandbioinformaticstoolstolocatethegenomicregionrelatedtorustresistanceinchickpea(CicerarietinumL.).CaballoC.1,FloresA.2,GilJ.2,MadridE.3,RubioJ.1andMillanT.21-ÁreadeMejorayBiotecnología.IFAPA.Apdo3092.14080Córdoba,Spain.2 - Dept. of Genetics, University of Córdoba, Campus Rabanales Ed. C-5, 14071Córdoba,Spain.3 - Max Planck Institute for Plant Breeding Research Department of PlantDevelopmentalBiology.Carl-von-LinneWeg10,D-50829Cologne,GermanyChickpea rust is caused by Uromyces ciceris-arietini. In previousstudiesagenethatcontrolsresistancetochickpearustwasidentifiedin the linkage group 7 (LG7) of the chickpea geneticmap. Howeverthephysicalpositionofthisgeneisstillunknown.Inordertocomparethe genetic andphysicalmaps a groupofmicrosatellites distributedacross the chromosome 7 were selected following according tochickpea database (cicarmisatdb.icrisat.org). A recombinant inbredline(RIL)populationderivedfromaninterspecificcrossbetweenCicerarietinum (ILC72) x Cicer reticulatum (Cr5-10), susceptible andresistanttorust,respectively,wereusedforlinkageanalysis.SixoutoftwentymicrosatelliteswerepositionedinLG7.Theseresultsallowus tonarrow thearea related to the resistance.Nevertheless,additional markers should be included in this region in order todevelopfinemappinginthisarea.These findings could be the starting point for a Marker-AssistedSelection (MAS) programme for rust resistance in chickpea andidentifycandidategene.Acknowledgement:thisworkhasbeensupportedbytheINIAprojectcontractRTA2013-00025,co-financedbytheEuropeanUnionthroughthe ERDF 2014-2020 “Programa Operativo de CrecimientoInteligente”

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P158–S18 OverexpressionofaphytocystatinleadstodelayedsenescenceandenhanceddroughttoleranceinsoybeanMakgopaM.E.1,3,QuainM.D.1,2,Marquez-GarciaB.1,4,KunertK.J.1,3,FoyerC.H.11-CentreforPlantSciences,FacultyofBiology,UniversityofLeeds,Leeds,LS29JT,UK.2-CSIR,CropsResearchInstitute,Kumasi,Ghana.3-DepartmentofPlantSciences,UniversityofPretoria,Pretoria0002,SouthAfrica.4-VIBDepartmentofPlantSystemsBiology,GhentUniversity,9052Gent,Belgium.Cysteine proteinases and their naturally occurring inhibitors,cystatins, have many functions that remain poorly characterised,particularlyincropplants.Senescenceischaracterisedbyincreasesinproteolytic enzymes involved in various processes in the plant’sresponses to stress. Transgenic soybean plants over-expressing acysteine protease inhibitor (OC-I) were successfully generated andcharacterized. Plants of transgenic lines had differential transgeneexpression. Transgenic lines had lower protease activity determinedby an in-gel assay using SDS-PAGE. Transgenic lines retained morechlorophyll than controls at later stages of development. Underdrought stress, transgenic lines performed better CO2 assimilation(photosynthesis) and instantaneous water-use efficiencies (IWUE)thanwild-typeplants. Results obtained in this studyhave shownanimportant role of cysteine proteases in plant growth anddevelopment and plant stress. Futureworkwill focus on identifyingthese OC-I sensitive proteases and investigating their individualfunctioninplantgrowthanddevelopmentandstress.

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P159–S18 Interactionsbetweensoilwatercontent,stomatalconductanceandabscisicacidcontentoffieldpeacultivarssubmittedtodroughtstressPetrovićG.1,StikićR.2,ŽivanovićT.2,Vucelić-RadovićB.2,Šurlan-MomirovićG.2,ĐorđevićV.1,PetrovićI.21-InstituteofFieldandVegetableCrops,NoviSad,Serbia.2-FacultyofAgriculture,UniversityofBelgrade,Belgrade,Serbia.Drought is one of the main abiotic stresses limiting agriculturalproductionofmany importantcrops.Plantsrespondtodroughtstressby closing their stomata and reducing transpiration water loss. Thedrought stress experimentwas conducted on seven selected cultivarsoffieldpeaincontrolconditions(phytotronchamber).Theplantswereirrigatedattheoptimalsoilwatercontentuntilthethirdpairofleavesbeganemergence,afterwhichsoildroughtwasinducedbyterminationof irrigation up to 18% (moderate drought), and 9% substrate watercontent (severedrought).Controlplantswere irrigatedat theoptimallevel of soil water regime (36% substrate water content). Ameasurement of stomatal conductance and ABA concentration wasperformed.Thestomatalreactionsdifferedbetweencultivars,butonlyundermoderatedrought conditions,while all cultivars reactedby thefasterstomatalclosureunderseveredrought.ThecorrelationbetweenABA in leaves and changes in stomatal conductance revealed thatstomatal conductance in cultivar Dukat was most affected by theaccumulation of ABA in leaves (10 x increase) and that this reactioncould be explained by "chemical signals" of drought. Contrary to theprevious,the loweststomatalsensitivityto increasedconcentrationofABA(2.5xincrease)incultivarJavorindicatesthe"hydraulicsignals"ofdrought.

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P160–S18 UseofMACEtechnologytoidentifypositionalandexpressionalcandidategenesforresistancetoDidymella

pinodesinpeaWinterP.1,RubialesD.2,FondevillaS.21 - GenXPro GmbH, Altenhöferallee 3, 60438, Frankfurt am Main, Germany.pwinter@genxpro.de2-InstituteforSustainableAgriculture-CSIC,Avd.MenendezPidals/n,14004,Cordoba,Spain.MACE(MassiveAnalysisofcDNAEnds)allowsultra-deeptranscriptionprofiling without PCR introduced bias by combining second-generationsequencingandtheTrueQuanttechnology.InMACEeachcDNAmolecule is represented by one sequence (the tag) of 94 bp,originatingfromaregionaround100–500bpfromthe3’(poly-A)endof the transcript. High-throughput sequencing of tags providesnumerical gene expression values and allows the identification ofSNPs and INDELS from the 3’ends of the genes. In the frame of“LEGATO” project, we are using MACE to identify positional andexpressional candidate genes for resistance toDidymella pinodes inpea.MACEhasbeenusedtoperformadetailedtranscriptomicstudyto identify genes differentially expressed in a resistant reaction(accession P665) compared to a susceptible one (cv. Messire). Theresultingsequenceswillbeusedto identifySNPsbetweenP665andMessire for thesedifferentially expressed genes. These SNPswill befurther genotyped in the RIL population P665 x Messire in a highthroughputway by sequencingMACE libraries from 94 RIL families.Thiswillallowthemappingofthesedifferentiallyexpressedgenesinthe P665 xMessire geneticmap and the study of their possible co-localization with the QTLs associated with resistance to D. pinodesthathavebeenalreadyidentifiedinthispopulation.ThisresearchisfundedbytheEuropeanCommunity(FP7/2007-2013)grantagreementFP7-613551,LEGATO.

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P161–S18 AnupdateonhostplantresistanceandracescenarioofFusariumoxysporumf.sp.ciceris,Fusariumwiltpathogenofchickpea(CicerarietinumL.)inIndiaSharmaM.ICRISAT,IndiaTheseverity&yieldlossesofFusariumwilt(F.oxysporumf.sp.ciceris(Foc)differfromplacetoplaceowingtoexistenceofG×Einteractions& physiological races. Attempts have been made to identify stablesources of resistance through multi-location testing & to assesscurrent race scenarioof Foc. Chickpeawilt nursery, consistingof 27highlyresistantlinestowilt,wastestedthroughmulti-location/multi-yearevaluationathot spot locations.ANOVA indicatedvariability inwilt incidence due to genotypes, environments, & G×E. GGE biplotidentified stable genotypes/discriminating environments for FW.Completeresistancetogenotypesacrossthelocationswasnotfound;4 lines (ICCV05527, ICCV05528, ICCV96818, ICC11322) with stableresistance were found. Foc isolates (110) collected from diversegeographicallocationsinIndiawerecharacterizedusingstandardhostdifferentials. DArT markers were developed & their utility ingenotypingof110isolatesweredemonstrated.DArTarraysrevealedtotal 1,813 polymorphic markers. Cluster analysis/ PCA/populationstructureindicatedthatisolatesofFocwerepartiallyclassifiedbasedon geographical location. DArT markers were able to group theisolates in consistent with phenotypic grouping. Race-specificunique/rare alleles were detected. Study generated significantinformation&canbeusedfurtherfordevelopment&deploymentofregion-specificresistantcultivarsofchickpea.

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P162–S18 MorphologicalandmolecularcharacterizationsofAscochytablightcausedbyAscochytarabieiinCiceranatolicumSariD.1,BergerJ.2,KahramanA.3,AydoganA.4,CanC.5,BukunB.6,PenmetsaR.V.7,vonWettbergE.J.8,TokerC.91 - Akdeniz University, Faculty of Agriculture, Department of Field Crops, Antalya,Turkeyduygusari@akdeniz.edu.tr2-CSIRO,PlantInd.Wembley,Australia3 - Harran University, Faculty of Agriculture, Department of Field Crops, Sanliurfa,Turkey4-FieldCropsCentralResearchInstitute,Ankara,Turkey5-GaziantepUniversity,FacultyofArtandScience,DepartmentofBiology,Gaziantep,Turkey6-DicleUniversity,FacultyofAgriculture,DepartmentofPlantProtection,Diyarbakir,Turkey7-UniversityofCalifornia,DepartmentofPlantPathology,Davis,USA8-FloridaInternationalUniversity,DepartmentofBiologicalSciencesandInternationalCenterforTropicalBotany,Miami,Florida,USA9-University,FacultyofAgriculture,DepartmentofFieldCrops,AntalyaChickpea(CicerarietinumL.),theonlycultivatedspeciesinthegenusCicer L., is individually themost important pulse crop of theworld.Cultivated chickpea is susceptible to Ascochyta blight caused byAscochyta rabiei (Pass.) Lab. This is one of themajor biotic stressescausing yield losses of up to 100% and having a global distribution.Ascochyta blight affects negatively all aerial parts of bothdomesticatedchickpeaanditswildrelatives.CharacteristicAscochytablightlesionswereobservedonCiceranatolicumALEF.,whichisoneof the perennial wild relatives of chickpea, growing in the SouthEastern Anatolia Region, Turkey. Plants showed leaves, petioles,stems, and pods with brown lesions. Symptoms on leaves, stems,podsandseedswereevaluatedmorphologicallytoidentifytheblightdisease.Infectedsampleswerecollectedandtakentothelaboratoryforthemolecularcharacterization.Firstly,sampleswereincubatedtopotato dextrose agar (PDA) plates for understanding themorphological characteristics typicalofA. rabiei.DNAwasextractedfrom small pieces of fungus isolates using CTAB method. For thefunguscharacterization,internaltranscribedspacer(ITS)regions(ITS-1, 5.8S rDNA subunit, ITS-2) were amplified. The ITS sequence had≥99% nucleotide identity with the corresponding sequence inGeneBank for A. rabiei. The sequence was deposited in GenBank(AccessionNo.KU879335).Asa result, it is theeffectivemethod foridentificationofthefungus.

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P163–S18 Anintersubspecificgeneticmapinlentil:QTLidentificationforAscochytaresistance,andseedtraits.NeyraC.D.,Rey-BañosR.,MilianiA.S.,García-López,M.,SáenzdeMieraL.E.,PérezdelaVega,M.,GarcíaP.Dept.Genetics,UniversityofLeon,SpainAn intersubspecificgeneticmaphasbeendevelopedanalyzing94F7recombinant inbred lines obtained from a cross between Lensculinarissubsp.culinariscv.LupaandL.c.subsp.orientalis(BG16880)usingthesingleseeddescentmethod.Atotalof417geneticmarkersof several types, based in gene sequences, retrotransposons,transposons and anonymnous sequences, showed the expectedmendeliansegregation(1:1)andwereusedinthemapconstruction.The genetic map showed seven large linkage groups that seem tocorrespond with the seven chromosomes of lentil than and beenidentifieddue to theprevious studiesonsyntenybetweenLensandMedicago. Besides the 7 main linkage groups, several small onesappear, mainly including anonymous and/or retrotransposon basedmarkers.The geneticmap has been used to identifyQTL for several traits ofeconomical importancein lentilsuchasresistancetoAscochyta,andseedsizeandweight.AsmallnumberofQTLweredetectedforeachtrait (3 for Ascochyta resistance, 2 for seed weight and 3 for seedsize).

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P164–S18 EvaluationofyieldandmorphologicalcharactersofregisteredchickpeavarietiesindifferentecologicalconditionsofTurkeyMartD.EasternMediterraneanResearchInstitute,Adana,TurkeyRegistered chickpea varieties were tested in two different ecologicalconditions to evaluate their yield and adaptation parameters. Theexperiments were conducted in Cukurova-Adana located in theMediterraneanregionforwinterplantingandinErzurumlocatedintheEasternAnatoliaregionforsummerplanting,during2014-2016.Atotalof 34 registered chickpea varieties and17 genotypeswere evaluated.The highest and the lowest grain yield valueswere 17.1- 508.5 kg/daand 7.2 -441.8 kg/da for Experiment I and Experiment II in theCukurova-Adana location, respectively. Similarly the highest and thelowest grain yield values were 157.1- 131.5 kg/da and 129.6-136.8kg/da for Experiment I and for Experiment II respectively, in theErzurumlocation.ThisstudywassupportedbyTheScientificandTechnologicalResearchCouncilofTurkey(TUBITAK)withprojectnumber113O070.

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P165–S18 CytologicalandmolecularresponsesofPsAPY1-silencedpeatothehost-adaptedfungalpathogenMikiM.1,YamasakiS.1,YaoS.1,MatsuiH.1,NoutoshiY.1,YamamotoM.1,IchinoseY.1,ShiraishiT.1,2,ToyodaK.11-OkayamaUniversity,Japan2-ResearchInstituteofBiologicalSciences,Okayama,JapanOur research focusingon theplant cellwallwheremostpathogenicfungifirstmakecontactwithhostcellshavediscoveredtheecto-typeATP-hydrolyzing enzyme (apyrase; EC3.6.1.15) as a key player ofextracellular defense. In response to elicitor from Mycosphaerelapinodes, thecatalyticactivity inextracts fromthecellwallsofpea isenhancedinvitro,withtheconsequentincreaseofO2-generationbyperoxidase(s).Bycontrast,thesupprescinsAandB,virulencefactorssecreted by the same fungus, inhibit the apyrase as well as theperoxidase-catalyzedO2-generationinahost-specificmanner.Theseresults indicate that plant cell wall is capable of recognizing andresponding to incoming elicitormolecules and that the ecto-ATPasespatially regulates the apoplastic oxidative burst. Here we silencedPsAPY1geneencodingstheecto-ATPaseinpea,usinganApplelatentspherical virus-based virus-induced gene silencing. The PsAPY1-silenced peas (_PsAPY1) exhibited enhanced disease susceptibilityphenotype against infection by pycnospores ofM. pinodes. Indeed,the peroxidase-catalyzed O2- generation as well as expression ofPR10-mRNAwasimpairedwhenPsAPY1-silencedpeawasexposedtochitosanelicitor.Onthebasisoftheseresults,itislikelythatPsAPY1spatially regulates the apoplastic oxidative burst as well as thedownstream signaling that leads to expression of defense-relatedgenesinpea.

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P166–S18 ExtracellularapyrasePsAPY1inpearequiredforresistancetofungalandbacterialpathogensYamasakiS.1,MikiM.1,YaoS.1,MatsuiH.1,NoutoshiY.1,YamamotoM.1,IchinoseY.1,ShiraishiT.1,2,ToyodaK.11-OkayamaUniversity,Japan2-ResearchInstituteofBiologicalSciences,Okayama,JapanThe suppressors from Mycosphaerella pinodes inhibit the ecto-ATPase activity of host cell wall aswell as the peroxidase-catalyzedO2- generation, temporarily reducing the ability of the host cells todefend itself.Herewe silencedPsAPY1gene that encodes theecto-ATPase in pea, using an Apple latent spherical virus-based genesilencing. The PsAPY1-silenced peas (DPsAPY1) exhibited enhanceddiseasesusceptibilityevenagainst infectionbyanon-adaptedfungalpathogen, Colletotrichum higginsianum. Notably, on the surface ofPsAPY1-silenced pea, a hyphal tip-based entry (HTE), a recentlydiscovered way to enter the plant cells without appressorialformation,significantlyincreased,eventuallycausingnecroticspots.Asimilar result was also observed with Pseudomonas syringae pv.glycinea (non-pathogenic to pea), that the bacteria were able topropagate in the apoplatic space of PsAPY1-silenced pea. In ourseparate study we showed reduced responsibility of DPsAPY1 tochitosanand lipopolysaccharides (LPS),which is derived from fungalcell wall and glycolipid components of the outer membrane of theGram-negative bacteria, respectively. Indeed, the chitosan-inducedO2-generationaswellasexpressionofPR10-mRNAwas impaired inPsAPY1-silenced pea. It is thus likely that PsAPY1 spatially regulatesthe apoplastic oxidative burst as well as the downstream signalingleadingtoexpressionofdefense-relatedgenesinpea.

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P167–S18 FusariumredolensanewcausalagentoffusariumyellowingonchickpeainTunisiaBouhadidaM.*1,JendoubiW.*1,2,GargouriS.3,BejiM.1,4,KharratM.1,ChenW.5*Authorscontributeequally1-FieldCropsLaboratory,UniversityofCarthage,INRAT,RueHediKarray,2080Ariana,Tunisia.mariembouhadida@gmail.com2-NationalAgronomicInstituteofTunisia,43AvCharlesNicolle,1082Tunis,Tunisia3 -CropProtectionLaboratory,UniversityofCarthage, INRAT,RueHediKarray,2080Ariana,Tunisia4 - University of Tunis ElManar, Faculty of Sciences of Tunis, Campus University, ElManar,Tunis,Tunisia5-USDA-ARS,WashingtonStateUniversity,Pullman,WA99164,USAOne of the most important biotic stresses contributing to theinstability inchickpeayieldsisFusariumwilt,causedbythesoil-bornfungusFusariumoxysporumf.sp.ciceris(Foc).Thisdiseasehasbeenreported in most chickpea growing areas in Tunisia and over theworld causing significant annual yield losses under favorableconditions (SharmaandMuehlbauer2007). Thereareeight racesofthepathogen.Adiseasesurveywasconductedinordertodeterminethe races present and their distribution in Tunisia. Chickpea plantsshowing typical wilt symptoms in Tunisia were collected fromdifferent chickpea growing areas in 2013. Sequencing of thetranslationelongation factor 1α (TEF-1α) geneandPCRprotocols ofthe collected Fusarium isolates allowed quick and precisedifferentiation of F. oxysporum, F. redolens and F. oxysporum f. sp.ciceris. The association of F. redolenswithwilting-like symptoms inchickpea inTunisia isnoticed for the first time.Pathogenicityassaysusing3chickpeagenotypes(ILC482,JG62andcv.Beja1)andisolatesfromdifferentgeographicorigins indicated thatF. redolens ishighlyvirulentonchickpea.Fusariumredolens inducesadiseasesyndromesimilar to that caused by the yellowing pathotype of Foc. Studiesfocusing on identification of sources of resistance and genes/QTLsinvolved in the resistance to this newpathogenon chickpeawill beundertaken.

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P168–S18 Effectsofsaltstressongrowth,nodulationandnitrogenfixationofFababean(ViciafabaL.minor)cultivatedinAlgeriaChaker-HaddadjA.1,NabiF.2,LazaliM.2,SadjiH.1,OunaneS.M.21-LaboratoiredeBiologieetdePhysiologiedesOrganismes,EquipedeBiologieduSol,FacultédesSciencesBiologiques,UniversitédesSciencesetde laTechnologieHouariBoumediène,BP32,ElAlia,BabEzzouar,16111Alger,Algérie2-DépartementdePhytotechnie,EcoleNationaleSciencesAgronomiques,El-Harrach16200Alger,AlgérieThe two major environmental factors that currently reduce plantproductivityaredroughtandsalinity.Salinityinsoilorwaterisoneofthe major stresses especially in arid and semi-arid regions, and itcould severely limit crop production. In Algeria, more than 20% ofirrigatedlandsareaffectedbysalinity.Theaimofthisstudywastoevaluatetheeffectsof50and100mMNaCl concentrations on growth, nodulation, nitrogen fixation andaccumulation of proline of four faba bean (Vicia faba L. minor)cultivars growing symbiotically with Rhizobium leguminosarum bvviciae. The response to salt stressdependedon thecultivarand thelevelsalt.Thus,100mMNaClcausedagreatreduction indryweightof shoot and root. Number, dry weight of nodules per plant andnitrogenfixationwerereducedwithincreasingsalinityforallcultivars.However, proline content was increased in leaves of all cultivarstested. Sidi Aich and Espresso were less affected by salt than thesensitivecultivarsCastelandMaya.OurresultssuggestthattheNaCltolerance of the Sidi Aich and Espresso cultivars is supported bybetter growth, the less N2 fixation inhibition and a higher osmoticadjustment.

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P169–S18InteractiveexplorationoftheMedicagostressresponsethroughtime-seriesanalysisintheMetaboClustsoftwarepackageRusilowiczM.¹,²,DickinsonM.³,CharltonA.³,O’KeefeS.¹,²,WilsonJ.¹,⁴1-YorkCentreforComplexSystemsAnalysis,UniversityofYork2-DepartmentofComputerScience,UniversityofYork3-FeraScienceLtd.4-DepartmentsofMathematicsandChemistry,UniversityofYorkAsomics technologies suchasLC-MShave increased their throughputcapabilities, this has allowed the acquisitionof sample sizes sufficientfor meaningful time series analysis. Large scale plant breedingprograms such as ABSTRESS, an international project into biotic andabiotic plant stress, generate many mega-variate datasets. Whilst anumberofstatistical toolsandmethodologiesareavailable toanalysesuch data, it is not always apparent a priori which methods andparameterswillproducedesirableresults.Thisisespeciallytrueoflargescale,non-targetedstudies.HereweintroduceanovelsoftwaresolutioncalledMetaboClustforthepurposesofanalysingdatafromsuchstudies.WedemonstratethisbyanalysingtheeffectsofcombinedbioticandabioticstressinMedicagotruncatula,amodellegume.The non-targeted dataset comprises just under 2900 variables for 4experimental groups over 13 time-points, representing LC-MS spectraobtained from processed leaf samples for stressed and non-stressedplants over two weeks. The software assists the user in selectingappropriate parameters through visual and interactive feedback. Weuse this to select a suitable smoothing function from which we cancluster the time-profiles. The software allows these clusters to becompared with known metabolic pathways for Medicago, obtainedfromMedicCyc,with an aim to identify pathways potentially affectedbytheexperimentalconditions.Twosuchpathwaysarepresented.

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P170–S18GeneticdiversityofViciafabaL.landracesinMorocco:areservoirfortheenhancementofthecompetitiveabilitytoweedstressGhaoutiL.1,AqtbouzN.1,BelqadiL.1,LinkW.21 - Département de Production, Protection et Biotechnologies Végétales, InstitutAgronomiqueetVétérinaireHassanII,Rabat,Morroco2-DepartmentofCropSciences,Georg-AugustUniversität,Göttingen,GermanyFababean,themajorlegumecropinMorocco,isanancientcropwitha large extent of genetic diversity. Over 90% of the farmers arecropping landraces using traditional farming practiceswith very lowinputs.Theyield lossattributedtoweeds infababeancanamount60-70%.Mechanicalandchemicalweeding isveryrarelyused.Thehigh levelof genetic diversityofMoroccan fababean landraces constitutes anoptionforfarmerstocopewithweedstress.No study exists so far on the competitiveness of the Moroccanlandraces to weeds. The objective of this study is (1) to assess thegenetic diversity of the Moroccan populations and (2) to evaluatetheircompetitiveabilitytoweeds.A set of 60 Moroccan local populations, and 2 checks (Aguadulce;Defez) which are commonly used cultivars, were (1) analyzedmolecularly through AFLP to assess their genetic diversity and (2)tested under field conditions in 4 environments to assess theircompetitiveabilitytowardweeds.Asplit-plotdesignwasusedwith2treatments(with/withoutweeds).Thewhitemustardwasusedasamodelweed.AFLP analysis revealed a large diversity within (82%) and between(18%)thelocalpopulations.The analysis of variance showed that the differences betweengenotypes and treatments as well as their interactions weresignificantformaturity,yieldandyieldcomponents.

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P171–S18 PotentialofarbuscularmycorrhizalfungiandnitrogenfixingbacteriaforimprovingtheproductionofgrainlegumesunderwaterstressCarvalhoP.1,RochaI.2,MaY.2,NunesM.1,MarquesG.3,LátrA.4,VosátkaM.5,FreitasH.2,OliveiraR.S.1,21-DepartmentofEnvironmentalHealth,ResearchCentreonHealthandEnvironment,School of Allied Health Sciences, Polytechnic Institute of Porto, Vila Nova de Gaia,Portugal.2-CentreforFunctionalEcology,DepartmentofLifeSciences,UniversityofCoimbra,Coimbra,Portugal.3 - CITAB - University of Trás-os-Montes and Alto Douro (UTAD), Department ofAgronomy,VilaReal,Portugal.4 - Symbiom Ltd., Lanskroun, Czech Republic. 5 Institute of Botany, Academy ofSciencesoftheCzechRepublic,Pr_honice,CzechRepublic.Grain legumes arewidely cropped plantswith significant economic,social and environmental importance. Arbuscular mycorrhizal fungi(AMF)andnitrogenfixingbacteria(NFB)can improveplantnutritionand increase drought tolerance. The study aimed at assessing theeffectofAMFandNFBinoculationinpromotingthegrowthandyieldof a grain legume as a sustainable agricultural practice. Cowpea[Vignaunguiculata(L.)Walp.]wasinoculatedwithAMFandNFBandsubjectedtothreelevelsofwaterstress:nostress,moderatestressorseverestress.Inoculatedplantshadimprovedgrainyieldunderwaterstress when compared with non-inoculated controls. Plant growthwasalsosignificantly increasedininoculatedplants. InoculationwithAMFandNFB can improve theproductivityof grain legumes. Thesemicrobial inoculants can contribute for sustainable production ofheathyfoodunderadverseenvironmentalconditions.Acknowledgements: R. S. Oliveira, Y.Ma and I. Rocha acknowledgethesupportofFundaçãoparaaCiênciaeaTecnologia(FCT)throughthe research grants SFRH/BPD/85008/2012, SFRH/BPD/ 76028/2011and SFRH/BD/100484/2014 and Fundo Social Europeu (FSE). Thisworkwas financed by Portuguese national funds through ProgramaOperacionalCompetitividadeeInternacionalização(POCI)andFundoEuropeu de Desenvolvimento Regional (FEDER) under Project POCI-01-0145-FEDER-016801 and by FCT under Project PTDC/AGR-TEC/1140/2014.

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P172–S18Effectofpeaseed-bornemosaicvirus(PSbMV)Pathotype4onfieldpeaPascheJ.NorthDakotaStateUniversity,USAPeaseed-bornemosaicvirus (PSbMV; familyPotyviridae) causes largeyield and seed quality losses in field pea (Pisum sativum). Theincidence and severity of damage caused by the virus varies acrosscultivars and is influenced by pathotype, among other things. Theobjective of this research was to evaluate infection frequency ofPSbMV pathotype 4 (P4) and the effect of the virus on seedtransmission, symptoms,weight, andnumber per pod in 20 cultivars.PSbMVisolateND14-1,99%identicalinnucleotidesequencetoP4,wasobtained from infected fieldpea seedgrown inNorthDakota,USA in2014.Theisolatewasmechanicallyinoculatedontofieldpeacultivars5weeks after planting (WAP) and infection frequency was evaluatedusingenzyme-linkedimmunosorbentassay(ELISA)at8and11WAP.Ofthe cultivars evaluated, two were resistant (presumably containingsbm1 allele), one was moderately susceptible (25% infectionfrequency), and all others were susceptible (50-80% infectionfrequency) to PSbMV P4. Yield reductions up to 52% observed inPSbMVinfectedplants15WAPweremainlyattributedtoreducedseedsize.Inmanycultivars,seedandpodnumberincreasedduetodelayedplant maturity. Daughter seeds from infected plants were sorted bysymptoms and tested for PSbMV 5 WAP. No difference in seedtransmission was observed between symptomatic and asymptomaticseed, confirming that seed symptoms do not predict virus presence.SeedtransmissionofPSbMVP4rangedfrom0-52%.

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P173–S18DevelopmentofafreezingtestincontrolledconditionsforPisumsativum.HascoëtE.1,DevauxC.2,BejiS.3,JaminonO.1,DufayetV.4,LecomteC.4,DelbreilB.3,Lejeune-HénautI.11-USCICV-AFP,INRA,80203,Péronne,France2-USCICV-AFP,TerresUnivia,80203,Péronne,France3-USCICV-AFP,UniversitéLille1,59655,Villeneuved'Ascq,France4-UMRAgroécologie,INRA,21065,Dijon,FranceFreezing isamajorenvironmental limitationtocropproductivity foranumberof species including legumes. In thecontextof global climatechange,wintercropswillexperimentmilderautumntemperaturesthatcouldbedetrimental totheachievementofcoldacclimation,which isthe ability for plants to increase their level of frost tolerance (FT) inresponse to low but non-freezing temperatures. For the pea crop, amodellingapproachhasshownthatclimatewarmingwill increasetheoccurrenceof freezingdamageevents,even if these latterwillbe lesssevere (Castel et al., 2014). Thus, breeding for frost tolerant winterpeasrequiresnotonlyto improvetheirFTthreshold,butalsotoraisetheircoldacclimation rate. Inorder toevaluate thegeneticvariabilityof both traits, we are adjusting a protocol in controlled conditions,which provides an indirect evaluation of FT by the measurement oftissues’electrolyteleakage(EL).Peastemsampleshavebeencollectedafter variable durations of cold acclimation at 4°C day/2°C night in aclimaticchamber.Theyhavebeenthenprogressivelycooledat2°Ch-1in a programmable temperature-test chamber to reach testtemperatures ranging from +4°C to -36°C. After 14 days of coldacclimation, EL evaluation enabled the same ranking of genotypesaccordingtotheirFTthresholdasobtainedintheChaux-des-Présfieldplatform. Improvements of the protocol are however still needed touseitasaroutinerankingtest.

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P174–S18NestedAssociationMappingforresistancetoAphanomycesrootrotofpeainMedicagotruncatulaTollenaereR.1,HajriA.1,BonhommeM.2,BoutetG.1,YoungN.3,BarangerA.1,JacquetC.2,Pilet-NayelM.-L.11-INRA,UMR1349IGEPP,PISOM,UMTINRA/TerresInovia,LeRheuF-35653,France2-UPS-CNRS,LRSV,F-31326Castanet-Tolosan,France3-UniversityofMinnesota,SaintPaul,MN-55108,USAPeaisanimportantfoodcropworldwide,providingmorethan17Mtinthe year 2013-2014 (FAOSTAT, 2016). It is the most widely grownlegume crop in the European Union. The high nutritional content ofpeas, inparticular, theirproteincontentmakesthemadesirable foodsourceforbothhumansandlivestock.AmajordiseaseofthislegumeisAphanomycesrootrotcausedbythesoilborne oomycete pathogen, Aphanomyces euteichesDrechs. Thereare few methods known to effectively control this disease, whichimpactsgreatlyonproductivity.Thesearchforresistancetraitsinpeaisimperativeforincreasingyield.Medicago truncatula is used to study the legume/Aphanomycespathosystemasitiseasilymanipulated,thereisahighqualitygenomeavailable and it is a natural host of Aphanomyces. We used nestedassociationmappinganalysisofconnectedRILpopulationsofMedicagotruncatula in order to investigate the diversity of loci and candidategenesunderlyingquantitative resistance toAphanomyces root rot,bycomparison with previous QTL or genome-wide association studies.Resultswill providenewdata toanalyse thegenomic conservationofresistance loci between M. truncatula and pea and to identifycandidate genes underlying resistance QTL in pea using translationalgenomics.

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P175–S18 Characterizationoflow-strigolactonegermplasminpea(PisumsativumL.)resistanttocrenatebroomrape(OrobanchecrenataForsk.)PavanS.1,SchiavulliA.2,MarcotrigianoA.R.1,BardaroN.1,BracutoV.1,RicciardiF.2,CharnikhovaT.3,LottiC.2,BouwmeesterH.3,RicciardiL.1;1-DepartmentofPlant,SoilandFoodScience,SectionofGeneticsandPlantBreeding,UniversityofBari,viaAmendola165/A,70126Bari,Italy2 -DepartmentofAgriculture,FoodandEnvironmentalScience,UniversityofFoggia,viaNapoli25,71100Foggia,Italy;3-LaboratoryofPlantPhysiology,WageningenUniversity,Droevendaalsesteeg1,6708PBWageningen,TheNetherlandsCrenate broomrape (Orobanche crenata Forsk.) is a devastatingparasitic weed threatening the cultivation of legumes around theMediterraneanandintheMiddleEast.Sofar,onlymoderatelevelsofresistancewere reported to occur in pea (Pisum sativum L.) naturalgermplasm, and most commercial cultivars are prone to severeinfestation. Here, we describe the selection of a pea line highlyresistant to O. crenata, following the screening of local geneticresources.Timeseriesobservationsshowthatdelayedemergenceofthe parasite is an important parameter associated with broomraperesistance. High performance liquid chromatography connected totandem mass spectrometry (LC-MS/MS) analysis and in vitrobroomrape germination bioassays suggest that the resistancemechanism might involve the reduced secretion of strigolactones,plant hormones exuded by roots and acting as signallingmoleculesforthegerminationofparasiticweeds.Twoyearsreplicatedtrials innon-infestedfieldsindicatethattheresistanceisdevoidofpleiotropiceffectsonyield,incontrasttopeaexperimentalmutantsimpairedinstrigolactone biosynthesis, and thus is suitable for use in breedingprograms.

325

P176-S18 Flowerabscissionratesofearly-maturingsoybeanvarietiesAperJ.1,HaezebrouckJ.2,BralL.2,ReheulD.2,BaertJ.11-PlantUnit,InstituteforAgriculturalandFisheriesResearch,Belgium,2-FacultyofBioscienceEngineering,GhentUniversity,BelgiumNorth western Europe could decrease its heavy dependency onimported protein crops by a local production of soybean.Unfortunately, the region’s short and cold growing season is aseriouslimitationforhighsoybeanyields.Whenthefloweringtimeof early-maturing soybean varieties would start earlier, thepotential yield could be improved, but then cold tolerance duringflowering becomes more important. Therefore, the flowerabscission rates during the flowering periodwere evaluated in anobservationtrialwith8MG000soybeanvarietiesin2014and2015atanearlyandlatesowingdateinMerelbeke(Belgium)andin2014at a normal sowing date in Elsenborn (Belgium). We determinedfloweringtimeandcountedthenumberofbuds,youngflowers,oldflowers and pods on a weekly basis on nodes 4 to 9 during thebeginningofflowering(R1)tillthebeginningseedstage(R5).Theseobservations allowed us to calculate the abscission rates of thereproductiveorgans.Whentheabscissionratesofoldflowerswereplottedovertime,therewasaclearresponsetothecoldpeaksthathappenedbetweentwomomentsofobservation.Aftercoldevents,morethan80%of theold flowerswere lost.Coldeventswerenotrestricted to the beginning of the flowering period and returnedfrequently. The results of this study indicate that breeders shouldfocus on cold tolerance during flowering to increase the potentialyieldlevelofsoybeaninnorthwesternEurope.

326

P177–S18 ExploitingpublicSNPmarkersformappingdiseaseresistanceQTLinFababean(Viciafaba)GostkiewiczK.,WebbA.,ThomasJ.,WoodT.NIAB,Dept.FieldCropsResearch,CambridgeUKFababean(Viciafaba)hassufferedfromlessinvestmentinselectiveimprovementcomparedtoothermajorarablecrops.Therehasbeenlimiteddevelopmentinmarker-assistedbreedingstrategiesandalackofbasicgenomic informationconfounds fundamental investigations.UsingapublicsetoffabaKASPSNPmarkers(Webbetal.,2015)andgermplasm developed through the DEFRA Pulse Crop GeneticImprovement Network (PCGIN) we have created genetic mappingpopulationsto identifymarkers linkedtoquantitativetrait loci (QTL)contributing to resistance against Peronospora viciae f sp fabae,AscochytafabaeandBotrytisfabae.Linkedgeneticmarkerscouldbeexploitedbyplantbreeders,totrackimportantregionsofthegenometoefficientlyintrogressresistanceintonew,improvedvarieties.F2:F3populations segregating respectively for downy mildew andAscochyta resistance have been genotyped using a selection of SNPmarkers. This information has been used to construct geneticmapson which QTL will be located; inoculated screens in controlledenvironments have been used to phenotype F3 faba seedlings inorder to identify resistance QTL and provide plant breeders withgeneticmarkerswithutilityforenhancingdiseaseresistance.

327

P178–S18 MicrobialassociationswithlegumeplanttriggersystemicresistanceagainstDidymellapinodesinfectionDesalegnG.BOKU,ViennaSpeciesofascochytablightsuchasDidymellapinodes isoneofthemost damaging aerial pathogen of field pea (Pisum sativum. L)(Fondevilla et al., 2011; Tivoli and Banniza, 2007). It sometimesresultsinthelossoftheentirecrop,especiallyinmonocultureandwhenever there is a favourable environmental condition for itsinfection and further development. In a pot experiment,with andwithout D. pinodes infection tests were conducted to study theeffectsofthreesymbionts(Rhizobium,arbuscularmycorrhizafungiand co-inoculation of the two) and non-symbiotic controltreatments on pea in a completely randomized design with fourreplicates.After infectionwith thispathogen, leafmetabolitesandproteome, disease severity and shoot biomass and green areaswere analysed.We found significantly highest biomass and greenareas in pea plants inoculated with Rhizobium. Furthermore, theoverallregulationofthecitricacidcycle,aminoacidandsecondarymetabolismincludingthepisatinpathwayweremostpronouncedinrhizobiaassociatedplantswhichhadalso the lowest infectionrateand the slowest disease progression. The co-inoculation increasedthe synthesis of stress related proteins, while mycorrhizaltreatmentswereinvolvedinmetalionhomeostasisanddampeningof reactive oxygen species. Overall, we conclude that microbialassociations, Rhizobium bacteria in particular, modify theinteractionbetweenhostplantandtheaerialpathogen.

328

P179–S18DevelopmentofdifferentialsetstoidentifyUromycesviciae-

fabaepathotypesinAustraliaIjazU.,AdhikariK.,BansalU.,BarianaH.,TrethowanR.PlantBreedingInstitute,TheUniversityofSydneyFaba bean rust (Uromyces viciae-fabae) is an important disease insub-tropicalregionofAustraliaandpathogenvariabilityofthisfungusis unknown. Pathotypes (races, strains) are the unique variants of apathogenthatdifferintheirabilitytoovercometheresistanceinoneor more hosts of the same species. The ability or inability of rustpathogentoinfectagroupofhostgenotypesallowsthepathotypetobe detected. These genotypes are known as differentials. Thedifferential developmentworkhasbeendesignedandperformedatCerealRustTestingLaboratory,PlantBreedingInstitute,Cobbitty.Thepure rust urediospores suspended in light mineral oil (Isopar) weremistedonthesetofresistantplantsusingultralowvolumeapplicator(Microfit,Micronsprayer,UK).Afterinoculation,plantswerekeptforincubation indark roomwith100% relativehumidity for24h.Afterincubation,plantsweremovedinmicroclimateroomat24±2oCandseedlingswererated(0-4)fordiseaseresistance.Sevenseedlingrustresistantgenesweredetected in cultivarDoza selections#12034&14916, collected accession Ac1655, Ac1227#14908, Ac1257#14904and some advanced lines IX114#15033 and 585c/1-11 in Australia.These seven genotypes showed significant distinctvirulence/avirulence pattern against the collected rust isolates fromthreemajorfababeangrowingstates(NSW,QldandSA)inAustralia.The observed vir/avr pattern can be used to identify the currentpathogenvariables.

329

P180–S18 Phenotypingforirondeficiencychlorosisinsoybeanplantsatmorphological,biochemicalandmultispectrallevelSantosC.S.1,AraújoD.1,2,CarvalhoS.M.P.1,2,3,HajirezaeiM.-R.4,GiehlR.4,vonWirénN.4,VasconcelosM.W.11-CentrodeBiotecnologiaeQuímicaFina–LaboratórioAssociado,EscolaSuperiordeBiotecnologia,UniversidadeCatólicaPortuguesa,Porto,Portugal.2 - GreenUP/CITAB-UP & DGAOT, Faculty of Sciences, University of Porto, CampusAgráriodeVairão,RuaPadreArmandoQuintas,7.4485-661Vairão,Portugal.3 - Department of Plant Sciences, Horticulture and Product Physiology Group,WageningenUniv.,Droevendaalsesteeg1,6708PBWageningen,TheNetherlands.4-MolecularPlantNutrition,DepartmentofPhysiology&CellBiology,Leibniz-InstituteofPlantGeneticsandCropPlantResearch,Gatersleben,Germany.Iron (Fe) is an essential mineral nutrient for plant growth. Albeitpresent in the soil in high concentrations, it is often deficient foruptake in calcareous conditions, because it is mostly present in anoxidizedstate.Likemostcrops,soybean(Glycinemax.L) ispronetodeveloping IronDeficiency Chlorosis (IDC), a condition that severelyaffectsplantgrowth.Phenotypingplantsatawhole-plant,organandcellular levels is essential to understand themechanismsunderlyingFe uptake, trafficking and homeostasis and this integrated view isessential in order to prevent IDC. We conducted a phenotypingexperiment,underthescopeofanSTSM,wherewegrewasubsetofsoybean lines under Fe limitation and Fe sufficiency, collected andconcentratedtheir rootexudatesandsubjectedthemtoanalysis fororganic acids and Femobilization capacity.Wewereable to classifythe lines in terms of their Fe efficiency and the main factorcontributingfortheirbehaviour.Regardingtheseresults,weselectedanFeefficient lineandconductedanotherexperimentwhereplantswere germinated and grown for seven days under hydroponicconditionswithFesupplyandthen, inatimecoursetrial,theyweretransferredforFedeficiencyandgrownfor2,4,6and8daysunderFe deficiency. Plant growth was 25, 31, 46 and 15 % lower in Fedeficient plants (fresh weight) and total chlorophylls were alsodecreased in more than double when compared to the controlconditions. Also, at each time point, multispectral measurementswereperformedusingaXpeCAMX01camera,thereflectanceofthemaintrifoliateleafwasanalysedinawavelengthrangeof450to950nm and the correlations of these measurements and SPAD values(whichcorrespondtothevisualsymptoms)wereestablished.

330

P181–S18 IdentificationofpeaaccessionswithcombinedresistancetoFusariumwiltanddroughtRispailN.1,BaniM.1,2,RubialesD.11-InstituteforSustainableAgriculture,CSIC,Avda.MenéndezPidals/n,14004Cordoba,Spain2-EcoleNationaleSupérieuredeBiotechnologie,villeuniversitaireAliMendjeli,25100Constantine,AlgériePea,Pisum sativum L. is one of themajor legume cropsworldwide.However, its yield remains relatively low mainly due to its highsusceptibility to stresses. Pea is particularly susceptible to droughtand to Fusarium wilt caused by the soilborne fungal pathogenFusarium oxysporum, whose damage is favored by by warmtemperatures and dryweather. Loses caused by this pathogens arethus expected to be more severe and widespread in future due toclimate changes that also predicted an increase of drought proneareas. The use of resistant cultivars is currently the most efficientcontrolapproach.However,theexistingresistancelevelintroducedinlegume cultivar is still insufficient to control the disease. It is thuscrucial to identify and exploit new sources of resistance to F.oxysporum and drought. In the frame of the EU project, ABSTRESS,we initially established an optimum inoculation method suitable tostudy legume response to fusarium wilt and its interaction withdrought stress using the model legume Medicago truncatula. Thismethodologywas thenapplied to screena largePisumcollection toidentify pea accessions resistant to F. oxysporum. Twenty peaaccessionswerefoundhighlyresistanttoF.oxysporum.Theresponseof these resistant sources to combined fusarium wilt and droughtstresses were then tested under controlled condition allowingidentification of several accessions with high level of resistance tofusarium wilt and tolerant to drought. These sources of resistancewouldbeveryvaluabletointrogressresistancetobothstressesinpeacultivar.

331

P192–S18 Efficiencyofdifferenttraitsforphenotypingsoybeandroughttoleranceinanautomatedplatformwhenestimatedunderdifferentbasis.PeironeL.,Irujo,G.P.,Aguirrezábal,L.A.N.LaboratoriodeFisiologíaVegetal,UnidadIntegradaBalcarce(INTA-FCA,UNMdP),Argentina,ConsejoNacionaldeInvestigacionesCientíficasyTécnicas(CONICET).laguirre@mdp.edu.arAn automated platform was used to assess the efficiency ofdifferent traits for phenotyping drought tolerance, taking intoaccountonlyonebasis,i.e.heritability.Theaimofthisworkwastocompare the efficiency of different traits for phenotyping whenestimated under different basis. Seven soybean genotypes werestudiedunderwellwateredandwaterdeficit treatment (WD).WDstartedat33daysafteremergence (DAE),and finishedat57DAE.34 traits were measured before and after the onset of the WDmeasured in theGlyph platform, a low-cost automatic automatedplatform developed for soybean, which allows the evaluation ofgenotypes under precisely controlled water deficit condition. Theefficiency of these traits for phenotyping were estimated for thebasistimefromemergencetophenotypingii)coefficientofgeneticdetermination (CGD), sample size, measurement time andmeasurement cost. Traits with a high efficiency on time fromemergence(Transpirationefficiencyestimatedat13,20,27,33and38DAE,LeafAreaRatioandShootDryWeightat38DAE)werealsoefficient when estimated on basis cost, measurement time, andsample size. They showed intermediate values of CGD. Theframework applied in thiswork for a joint analysis of efficiencyofseveral traits under different basis could be helpful for selectingthosemoreefficient forphenotypinggiven thebottleneckof eachspecific case (e.g. capacity of the phenotyping platform, availablesensors,budget).

332

P191-S18 DeterminationofpeasgenotypesforyieldandyieldComponentsinCukurovaregionTürkeriM.,MartD.,YücelD.,KaraköyT.EastMediterraneanAgriculturalResearchInstitute,Andana,TurkeyThis research was carried out to determine the seed yield and yieldcomponents of some pea genotypes under Çukurova ecologicalconditions during 2014-2015 at East Mediterranean AgriculturalResearch Institute Doğankent Location. Genotypes and cultivars fromICARDAandMenemenGeneBankwereusedastrialmaterial.Accordingto the results of the observation at genotypes, were examinate plantheight,floweringnumberofday,seedyield,100seedweight.Accordingtotheresultsoftheanalyses;thehighestand lowestseedyieldvalues376.8–91.7kg/da,floweringnumberofdayvalues101.0-72.0day,plantheight values151.6-48.3 cm, 100 seed weight 24.5-15.5 gr wereobtained from genotypes. These results provide an initial step towardthe identification of (Pisum sativum L.) that may be useful for thedevelopmentofbreedingPisumsativumL.

333

P193–S18 DroughtstressresponsesofPortuguesecowpeaaccessionsCarvalhoM.1,Lino-NetoT.2,MatosM.3,4,CastroI.1,3andCarnideV.1,31 - Centre for Research and TechnologyofAgro-Environment andBiological Sciences(CITAB), University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real,Portugal.2-Biosystems&IntegrativeSciencesInstitute(BioISI),PlantFunctionalBiologyCenter(CBFP),UniversityofMinho,CampusdeGualtar,4710-057Braga,Portugal.3-DepartmentofGeneticsandBiotechnology,UniversityofTrás-os-MontesandAltoDouro(UTAD),5000-801VilaReal,Portugal.4 -Biosystems& IntegrativeSciences Institute (BioISI), SciencesFaculty,UniversityofLisbon,CampoGrande,1749-016Lisbon,Portugal.Cowpea (Vigna unguiculata L.Walp.) is pointed as one of themosttolerantgrainlegumecropstodrought.Asaresult,thiscrophasbeenconsideredasan idealmodel tostudy themolecularmechanismsofdrought tolerance.Toevaluateanddetermine the tolerance leveloftwo Portuguese cowpea accessions (Fradel and Pinhel), severalbiochemical and production parameters were determined in plantssubmitted to three water treatments (25%, 50% and 75% of fieldcapacity, FC) during 15 days. As control, a drought-sensitive (CB46)genotypewasused.As expected, plants subjected to 25% of FC treatment alwayspresented higher stress levels. However, the Portuguese accessionsseem to present a reduced inducible drought response than CB46genotype, as evaluated by biochemical parameters. The highconstitutive levels of proline present in both Portuguese accessionsmayhaveresultedinreduceddroughtstresslevels,detectedbyH2O2quantification and lipid peroxidation, or by anthocyanin levels, ascompared to susceptible control. The number of cowpea pods andseedsweightindroughtconditionswasmuchmoreaffectedinFradelthan in CB46. In contrast, Pinhel was the genotype that presentedhigher tolerance to drought concerning the same productionparameters.Although theevaluationof biochemical parametershasrevealed a weak inducible drought response of both Portuguesecowpea genotypes, they both differed in production parameters inrelationtothesusceptiblecontrol.

334

335

ListofParticipants

Lastnam

eFirstN

ame

Affiliatio

nEm

ailA

ddress

Posternº

Abde

lgue

rfi

Aissa

ENSA

ALG

IERS

aabdelguerfi@

yahoo.fr

P40–S4

Abug

aliyeva

Aigul

LLP"KazakhResearchInstituteofAgricultureand

PlantGrowing"

kiz_abugalieva@mail.ru

P117–S13

Acikgo

zEsvet

UludagUniversity

esvet@

uludag.edu.tr

P106–S13

Adam

Eveline

SaatzuchtGleisdorfGmbH

eveline.adam@gmx.at

P107–S13

Aden

iyiM

iche

al

Adefisan

UniversityofIbadan,Nigeria

adefisanadeniyi@

hotm

ail.com

Adeo

sun

IsaiahAdemola

UniversityofIbadan,Nigeria

vickolat@

gmail.com

Aguirrezab

al

Luis

UniversidadNacionaldeM

ardelPlata-CONICET,

Argentina

luisaguirrezabal@

gmail.com

P90–S11

Ahmad

Salm

an

UniversityofSargodha,Sargodha

salm

anahmad@uos.edu.pk

Aino

uche

Abdelkader

UniversitédeRennes1,UMRCNRSEcobio

kader.ainouche@univ-rennes1.fr

P190–S3

Akinde

le

SamuelTope

Olarok&Co

olarokco@gmail.com

Akog

or

UkporFriday

ElanLeagueInternationalLim

ited

engrfrifun@gmail.com

AlM

asmou

di

Yassine

Inra

yassine.alm

asm

oudi@

gmail.com

P7–S2

Alalad

eClement

Oyostatem

inistryofAgriculture

oyoagric@

gmail.com

Alizad

eh

Khoshnood

DrylandAgriculturalResearchInstitute

khoshnod2000@yahoo.com

P45–S7

Almeida

Nuno

ITQBNOVA

nalm

eida@itqb.unl.pt

P152–S18

Alsina

Ina

LatviaUniversityofAgriculture

Ina.Alsina@llu.lv

Amok

rane

Yanis

ENSA

Alger/ECOLABCNRS/INPT/U

PS

yanisamokrane@yahoo.fr

P14–S2

Anay

Ayse

EastM

editerraneanAgriculturalResearchInstitute

ayseanay@

hotm

ail.com

Annicchiarico

Paolo

CREA-CentreforFodderCropsandDairy

Productions

paolo.annicchiarico@crea.gov.it

Oral–S8

Aper

Jonas

ILVO

jonas.aper@

ilvo.vlaanderen.be

P176–S18

Araú

jo

Susana

ITQBNOVA

saraujo@itqb.unl.pt

P149–S18

Arlauskien

ėAušra

JoniškėlisExperimentalStationoftheLithuanian

ResearchCentreforAgricultureandForestry

arlauskiene.ausra@gmail.com

P137–S14

Arrib

asM

artin

ez

Claudia

INIA

arribas.claudia@inia.es

P51–S7

Atienza

Sergio

IAS-CSIC

sgatienza@ias.csic.es

P67–S8

Aube

rt

Gregoire

INRA

gregoire.aubert@dijon.inra.fr

Aulrich

Karen

ThünenInstituteofOrganicFarm

ing,FederalRes.

InstituteforRuralA

reas,ForestryandFisheries

karen.aulrich@thuenen.de

P47–S7

Baba

tund

eMoruffAbiodun

FetchesInternational

fetchesinter@

gmail.com

Bahceci

Hakan

InternationalPulseTradeandIndustries

Confederation

tuba.durgun@hakanfoods.com

KeyLect-1

Balliu

Astrit

AgriculturalU

niversityofTirana

aballiu@ubt.edu.al

P127–S14

Barang

er

Alain

INRA

Alain.Baranger@

rennes.inra.fr

Oral–S12

Barata

AnaM

aria

BPGV/INIAV

ana.barata@iniav.pt

P34–S4

Barcelos

Carina

INIAV

carina.barcelos@

iniav.pt

P36–S4

Barrad

as

Ana

Fertiprado

abarradas@

fertiprado.com

Bedo

ussac

Laurent

ENFA

/INRA

laurent.bedoussac@

toulouse.inra.fr

Oral–S5

Beji

Sana

UniversityLille1

sana.beji@ed.univ-lille1.fr

Oral–S18

Ben

Cecile

ECOLABCNRS/INPT/U

PS

ben@ensat.fr

P39–S4

Bene

zit

Maud

INRA

Maud.Benezit@

grignon.inra.fr

P123–S13

Bentaibi

Abderrahim

InstitutNationaldelaRechercheAgronomique,

Morroco

abentaibi@

yahoo.fr

Oral–S5

Bian

co

Carm

en

IBBR-CNR

carm

en.bianco@ibbr.cnr.it

P189–S3

Biarne

sVeronique

TerresInovia

v.biarnes@

terresinovia.fr

P125–S13

Bing

Dengjin

AgricultureandAgri-FoodCanada

dengjin.bing@agr.gc.ca

P105–S13

Blake

Sara

SouthAustralianResearchandDevelopment

Institute(SA

RDI)

sara.blake@sa.gov.au

Bobille

Hélène

URLEVA-ESA

,UMRSESA

N-IRHS,Univ.Angers

h.bobille@groupe-esa.com

P88–S11

Böhm

Herw

art

Thünen-InstituteofOrganicFarm

ing

herw

art.boehm@thuenen.de

P130–S14

Boros

Lech

PlantBreeding&Acclim

atizationInstitute-NRI

l.boros@

ihar.edu.pl

P73–S8

Bouh

adida

Mariem

CropsLaboratory,UniversityofCarthage,INRAT,

Tunisia.

mariembouhadida@gmail.com

P167–S18

Bourion

Virginie

INRA

virginie.bourion@dijon.inra.fr

Oral–S3

Boutet

Gilles

INRA

gilles.boutet@

rennes.inra.fr

Oral–S8

Bowne

ss

Robyne

AlbertaAgricultureandForestry

robyne.bowness@gov.ab.ca

P136–S14

Bozoğlu

Hatice

OndokuzMayısUnıversity

Bron

ze

Maria

ITQB/IBET

mbronze@itqb.unl.pt

Oral-S7

Brün

jes

Lisa

UniversityGöttingen,Germ

any

Lbruenj@

uni-goettingen.de

Oral–S15

Burstin

Judith

INRA,France

judith.burstin@dijon.inra.fr

KeyLec-8

Buurman

Milika

LimagrainUKLtd

milika.buurm

an@lim

agrain.co.uk

Caba

lloLinares

Cristina

IFAPA

cristinacaballolinares@

gmail.com

P157–S18

Cabe

llo-Hurtado

Francisco

UniversityofRennes1

francisco.cabello@univ-rennes1.fr

Campa

Ana

SERIDA

acampa@serida.org

Oral–S10

Campo

sFelisbela

Syngenta|SeedCare

felisbela.campos@

syngenta.com

Can

Canan

University

can@gantep.edu.tr

Caré

Rita

CiB-CentrodeInform

açãodeBiotecnologia

gabcom@cibpt.org

Carlsson

Georg

SwedishUniversityofAgriculturalSciences

georg.carlsson@slu.se

Oral–S14

Carović-Stan

ko

Klaudija

UniversityofZagreb,FacultyofAgriculture,

DepartmentofSeedScienceandTechnology

kcarovic@

agr.hr

P22–S4

Carton

Nicolas

URLEVA-ESA

n.carton@groupe-esa.com

Oral–S16

Carvalho

Márcia

CITAB,UniversityofTrás-os-MontesandAltoDouro,

5000-801VilaReal,Portugal

marciac@

utad.pt

Oral–S4

Castro

Isaura

CITAB,UniversityofTrás-os-MontesandAltoDouro,

5000-801VilaReal,Portugal

icastro@utad.pt

P193–S18

Ćeran

Marina

InstituteofFieldandVegetableCrops

marina_tomicic@yahoo.com

P112–S13

Chaker-Had

dadj

Assia

UniversitédesSciencesetdelaTechnologieHouari

Boumedienne,Algérie

assiachaker@

yahoo.fr

P168–S18

Charlto

nAdrian

FeraScienceLim

ited

adrian.charlton@fera.co.uk

Oral–S13

Chatterton

Syama

AgricultureandAgri-foodCanada

syama.chatterton@agr.gc.ca

Oral–S6

Chen

Weidong

USD

AARS

weidong.chen@ars.usda.gov

Oral–S12

Cimmino

Alessio

UniversityofNaplesFedericoII(UNINA)

alessio.cim

mino@unina.it

P98–S12

Clem

ente

Alfonso

EstacionExperimentaldelZaidin(CSIC)

alfonso.clemente@eez.csic.es

Oral–S9

Cnop

sGerda

ILVO

gerda.cnops@

ilvo.vlaanderen.be

P108–S13

Collado

Elena

UniversidadPolitecnicaDeCartagena

elenacolladomarin@hotm

ail.com

P146–S18

Corre-He

llou

Guenaelle

ESA

g.hellou@groupe-esa.com

Oral–S16

Costa

Sara

ITQBNOVA

smcosta@itqb.unl.pt

P81–S8

Coyn

eClarice

USD

AARS

clarice.coyne@ars.usda.gov

Oral–S6

Cupina

Branko

UniversityofNoviSad,FacultyofAgriculture

cupinab@polj.uns.ac.rs

Oral–S2

Dadu

Rama

UniversityofMelbourne

rdadu@student.unim

elb.edu.au

Davidson

Jenny

SARDI

jenny.davidson@sa.gov.au

Deok

ar

Amit

UniversityofSaskatchewan,Canada

aadeokar@

gmail.com

P71–S8

Desalegn

Getinet

BOKU,Vienna

getinet.desalegn@boku.ac.at

P178–S18

Dickinson

Michael

FeraScienceLim

ited

michael.dickinson@fera.co.uk

Oral–S18

Djordjevic

Vuk

Instituteoffiledandvegetablecrops

vuk.djordjevic@

ifvcns.ns.ac.rs

P111–S13

Dobrán

szki

Judit

ResearchInstituteofNyíregyházaUniversityof

Debrecen

dobranszki@freemail.hu

Domon

ey

Claire

JohnInnesCentre

claire.domoney@

jic.ac.uk

Oral–S7

Dostálov

áRadmila

AGRITEC,Research,BreedingandServices,Ltd.

dostalova@agritec.cz

P120–S13

Duarte

Isabel

INIAV

isabel.duarte@iniav.pt

P118–S13

Dubo

is

Gaëtan

EuropeanCommission-DGAGRI

gaetan.dubois@ec.europa.eu

Oral–S1

Duc

Gérard

INRA

gerard.duc@

dijon.inra.fr

Duckworth

Randall

GlobalPulseConfederation(CICILS/IPTIC)

r.duckworth@cicilsiptic.org

Duqu

eAnaSofia

ITQBNOVA

sduque@itqb.unl.pt

P61–S7

Ellis

Noel

thnoelellis@

gmail.com

KeyLec-4

Emmric

hPeter

JohnInnesCentre

peter.emmrich@jic.ac.uk

P80–S8

Eviden

te

Antonio

UniversityofNaplesFedericoII(UNINA)

evidente@unina.it

P99–S12

Ezekwe

Innocent

UniversityofIbadan,Nigeria

vickolat@

gmail.com

Farkas

Rita

AMBISLtd.

farkas.ritadiana@gmail.com

Feinstein

Alina

NRGene

alina@nrgene.com

Fernan

des

Hortense

UTAD-DepartamentodeAgronomia

hortenseafernandes@

gmail.com

Fernán

dez

JuanA

UniversidadPolitécnicadeCartagena

juan.fernandez@

upct.es

P20–S4

Ferrán

diz

Cristina

IBMCP,CSIC-UPV,Valencia,Spain

cferrandiz@ibmcp.upv.es

P84–S8

Ferreira

Juan

SERIDA

jjferreira@serida.org

P151–S18

Fevereiro

Pedro

ITQBNova

psalema@itqb.unl.pt

Fierro

Marco

UniversitàdegliStudidelM

olise

marco.fierro@studenti.unim

ol.it

P23–S4

Fische

rKristin

JuliusKühn-Institut,InstituteforBreedingResearch

onAgriculturalCrops

kristin.fischer@

julius-kuehn.de

Oral–S4

Fond

evilla

Sara

InstituteforSustainableAgriculture-CSIC

sfondevilla@ias.csic.es

P140–S18

Fran

cis

Stephen

PGRO/FenPeas

Stephen@fenpeas.com

Frazoa

Sofia

SoulTrails

sofia.frazoa@gmail.com

Füchtbau

er

Winnie

SejetPlantBreeding

wsf@sejet.com

Fülöp

Katalin

Bonafarm

BólyZrt.

Jozsefne.Fulop@boly.bonafarm

.hu

Fustec

Joelle

EcoleSupérieured'Agriculture,URLEVA,Angers,

France

j.fustec@

groupe-esa.com

P187–S3

Gaad

Djouher

NationalH

ighSchoolofAgriculture

gaad_djouher@

yahoo.fr

P18–S4

Galob

urda

Ruta

LatviaUniversityofAgriculture

galoburda@yahoo.com

P58–S7

Gan

ci

Patrizia

EuropeanCommission

patrizia.ganci@ec.europa.eu

Garcia

Pedro

Dept.Genetics,UniversityofLeon

pgarg@unileon.es

P163–S18

Gaw

łowska

Magdalena

InstituteofPlantGenetics,PolishAademyofSciences

mgaw@igr.poznan.pl

P148–S18

Gen

tzbittel

Laurent

ECOLABCNRS/INPT/U

PS

gentz@ensat.fr

Oral–S6

Gha

outi

Lamiae

InstitutAgronomiqueetVétérinaireHassanII

lamiaeghaouti@hotm

ail.com

P170–S18

GhislainLudo

vic

Mbemba

CooperativeAgriculteurMaty

cooperativeagriculteurm

aty@gmail.com

Gho

ulam

Cherki

FacultyofsciencesandTechniques,CadiA

yyad

University

c.ghoulam@uca.m

a

P185–S3

God

inho

Bruno

Fertiprado

bgodinho@fertiprado.com

Gom

ezdiM

arco

Perla

UniversidadPolitecnicaDeCartagena

perla.gomez@

upct.es

P43–S7

Gon

çalves

Letice

ITQBNOVA

letice.let@

gmail.com

P25–S4

Gragera-Facun

do

Juan

CICYTEX-LaOrden,JuntadeExtremadura,Spain

juan.gragera@gobex.es

P133–S14

Guillamon

Eva

INIA

guillamon.eva@inia.es

P52–S7

Guind

on

María

IICAR-CONICET(InstitutodeInvestigacionesen

CienciasAgrariasdeRosario)

ferguindon@gmail.com

P121–S13

Guine

tMaé

AgroSupDijon

mae.guinet@

@dijon.inra.fr

Oral–S16

Gup

ta

Soma

IndianAgriculturalResearchInstitute,NewDelhi,

India

somugupta24@gmail.com

P115–S13

GutierrezLeiva

Natalia

ZAYINTEC-IFA

PA

natalia.gutierrez@

zayintec.com

Guy

Stephen

WashingtonStateUniversity

sguy@

wsu.edu

Oral–S16

Hamwieh

Aladdin

ICARDA

a.hamwieh@cgiar.org

Harzic

Nathalie

Jouffray-Drillaud

nharzic@jouffray-drillaud.fr

Hascoë

tEmilie

INRA

Emilie.Hascoet@

mons.inra.fr

P173–S18

Hassissene

Nadia

UniversityofBejaia,Algerie

nadhassi1@gmail.com

P63–S7

Heshmati

Sara

Georg-August-UniversitätGöttingen

sarah.heshmati@agr.uni-goettingen.deP11–

S2

Hing

ane

Anupama

InternationalCropsResearchInstituteforSemi-Arid

Tropics

h.anupama@cgiar.org

P126–S13

Holdt

Courtney

NorthDakotaStateUniversity

courtney.holdt@

ndsu.edu

P6–S2

Hu

Jinguo

USD

A/ARS

jinguo.hu@ars.usda.gov

Huna

dy

Igor

AGRITECPLANTRESEARCHLtd.

hunady@

agritec.cz

P62–S7

Hybl

Miroslav

CropResearchInstitute

hybl@

genobanka.cz

P9–S2

Iann

etta

Pietro

TheJamesHuttonInstitute

pete.iannetta@hutton.ac.uk

Oral–S1

Ijaz

Usm

an

TheUniversityofSydney,Australia.

usm

an.ijaz@

sydney.edu.au

P179–S18

Infantino

Alessandro

CREA-Centrodiricercaperlapatologiavegetale

alessandro.infantino@crea.gov.it

Isho

la

Alabi

Anim

alScienceDepartment,Universityof

Ibadan,Nigeria

vickolat@

gmail.com

Jackson

Scott

CenterforAppliedGeneticTechnologies,USA

/

UniversityofGeorgia

sjackson@uga.edu

KeyLec-13

Jallo

w

Assiatou

PHARMACUETICAL

spaceworld.technology@

yahoo.com

Jallo

w

Momodou

UniversityoftheGambia

technical.alasana.sos@

gmail.com

Jensen

ErikSteen

SwedishUniversityofAgriculturalSciences

erik.steen.jensen@slu.se

Oral–S17

Jeuffroy

Marie-Hélène

INRA,France

jeuffroy@

grignon.inra.fr

KeyLec-2

Jha

Ambuj

UniversityofSaskatchewan

ambuj.jha@usask.ca

Oral–S9

Jhajj

Kuldeepr

PunjabAgriculturalU

niversity,Ludhiana,Punjab

(India)

kuldeepjhajj89@gmail.com

P12–S2

Jimen

ez-Lop

ez

JoseC.

CSIC,EstacionExperimentaldelZaidin;Department

ofBiochemistry,CellandM

olecularBiologyofPlants

josecarlos.jimenez@

eez.csic.es

Oral–S7

Journe

tEtienne-Pascal

INRA-CNRS

etienne-pascal.journet@

toulouse.inra.frO

ral–S16

Julier

Bernadette

INRA

bernadette.julier@

lusignan.inra.fr

Oral–S13

Justes

Eric

INRA,France

Eric.Justes@

toulouse.inra.fr

KeyLec-14

Kadziuliene

Zydre

LithuanianResearchCentreforAgricultureand

Forestry

zkadziul@

lzi.lt

P65–S7

Kafada

rFeyza

UniversityofGaziantep

incik@

gantep.edu.tr

Kalo

Peter

NARICAbriculturalBiotechnologyInstitute

kalo@abc.hu

P79–S8

kamara

Sewa

rokuprfooddevelopmentproject

gibrillaff02@gmail.com

Karayel

Reyhan

BlackSeaAgricultureResearchInstitute

reyhank55@hotm

ail.com

P60–S7

Karki

Nar

ChitawanEcoAgroPvt.Ltd.

chitwan.ecoagro1@gmail.com

Katanski

Snežana

IFVCNS,InstituteofFieldandVegetableCrops,Novi

Sad

snezana.katanski@

nsseme.com

P57–S7

Kaur

Sukhjiwan

AgriBio,AgricultureVictoria,DEDJTR

sukhjiwan.kaur@

ecodev.vic.gov.au

Oral–S8

Keller

Jean

UniversityofRennes1

jean.keller@

univ-rennes1.fr

Oral–S3

Kemal

Seid

ICARDA

s.a.kemal@

cgiar.org

KeyLec-12

Khah

lu

Tshepang

UniversityofPretoria

tkhahlu@gmail.com

P87–S11

Kham

assi

Khalil

InstitutNationaldeRechercheAgronomique

khkobe@hotm

ail.com

P78–S8

Kimbe

rRohan

SARDI

rohan.kim

ber@

sa.gov.au

Kisha

Theodore

USD

A-ARS

theodore.kisha@ars.usda.gov

P29–S4

Kiss

Gyorgy

AMBISLtd.

kiss.gyorgybotond@gmail.com

P107–S13

Knotov

aDaniela

AgriculturalResearch,Ltd.

knotova@vupt.cz

P48–S7

Knud

sen

Jens

NordicSeed

jeno@nordicseed.com

Kono

sono

ka

Inara

LatviaUniversityofAgriculture

inara.konosonoka@gmail.com

P49–S7

Kritzinger

Quenton

UniversityofPretoria

quenton.kritzinger@

up.ac.za

P8–S2

Kroc

Magdalena

InstituteofPlantGenetics,PolishAcademyof

Sciences,Poznan,Poland

mkro@igr.poznan.pl

P139–S14

Kron

Roberto

AGRIFUTUR

RKM@Agrifutur.com

Kron

berga

Arta

InstituteofAgriculturalResourcesendEconomics

Arta.Kronberga@arei.lv

P21–S4

Książkiewicz

Michał

InstituteofPlantGeneticsofthePolishAcademyof

Sciences

mksi@

igr.poznan.pl

P72–S8

Kuop

pala

Kaisa

NaturalResourcesInstituteFinland(Luke)

kaisa.kuoppala@luke.fi

P46–S7

Kushwah

Ashutosh

PunjabAgriculturalU

niversity,Ludhiana,Punjab,

India

anshu.kushwah24@gmail.com

P114–S13

Kwaten

gGeorge

Swedishuniversityofagriculturalscience

jean_kw

atson@yahoo.co.uk

P3–S2

Laou

ar

Meriem

ENSA

ALG

IERS

laouar_m@yahoo.fr

P122–S13

Lazali

Mohamed

UniversityofKhemisM

iliana

m.lazali@

yahoo.fr

P153–S18

Lazarević

Boris

FacultyofAgriculture,UniversityofZagreb

blazarevic@

agr.hr

LeM

ay

Christophe

Agrocampus,INRA

lemay@

agrocampus-ouest.fr

P96–S12

LeSigno

rChristine

INRA

clesignor@

dijon.inra.fr

Oral-S10

Leitã

oSusana

ITQBNOVA

sleitao@itqb.unl.pt

P147–S18

Lejeun

e-He

naut

Isabelle

INRA

Isabelle.Lejeune@mons.inra.fr

Lemken

Dominic

UniversityGöttingen

dlemken@gwdg.de

Oral–S5

Lepe

tit

Marc

INRALSTM

lepetit@

supagro.inra.fr

Oral–S3

Lepse

Liga

PūreHorticulturalResearchCentre,Latvia

liga.lepse@puresdis.lv

P19–S4

Lesné

Angélique

INRA

angelique.lesne@rennes.inra.fr

P113–S13

Ling

ner

Annika

UniversityofGoettingen,Germ

any

alingne@agr.uni-goettingen.de

Oral–S14

Link

Wolfgang

Georg-August-UniversitätGöttingen

wlink@

gwdg.de

Lope

ra-Cardo

na

Seneida

UniversityofAntioquia

seneida.lopera@yahoo.com

P53–S7

Lotti

Concetta

UniversityofFoggia

concetta.lotti@

unifg.it

P38–S4

Loua

rn

Gaëtan

INRA

gaetan.louarn@lusignan.inra.fr

Oral–S11

Lund

Bente

NorddeutschePflanzenzuchtHans-GeorgLembkeKG

b.schaper@

npz.de

Mad

havan

Ramakrishnan

WorldVegetableCenter

ramakrishnan.nair@worldveg.org

Oral–S15

Mad

ueño

Francisco

IBMCP,CSIC-UPV,Valencia,Spain

madueno@ibmcp.upv.es

Magrin

iMarie

INRA

mbmagrini@

toulouse.inra.fr

Oral–S5

Makgo

pa

Eugene

UniversityofPretoria

eugene.m

akgopa@up.ac.za

P158–S18

Marais

Julie

INRA

julie.m

arais@dijon.inra.fr

P56–S7

Marciniak

Katarzyna

CentreforModernInterdisciplinaryTechnologies,

NicolausCopernicusUniversityinTorun,Poland

marciniak@

umk.pl

P91–S11

Mariam

Yusufu

UniversityofIbadan,Nigeria

adefisanadeniyi@

hotm

ail.com

Marie

SiwoFoko

SHUMASCAMEROON

gci_cameroon@yahoo.com

Markell

Samuel

DepartmentofPlantPathologyNorthDakotaState

UniversityFargo,ND58102U.S.A

samuel.markell@

ndsu.edu

P103–S12

Marqu

es

Guilherm

ina

UniversidadedeTrás-os-MonteseAltoDouro

gmarques@

utad.pt

P183–S3

Marshall

Athole

AberystwythUniversity

thm@aber.ac.uk

Marsolais

Frédéric

AgricultureandAgri-FoodCanada

Frederic.Marsolais@agr.gc.ca

KeyLec-7

Mart

Durdane

EastM

editerraneanAgriculturalResearchInstitute

durdanemart@yahoo.com

P164–S18

Martin

-Ped

rosa

Mercedes

INIA

mmartin@inia.es

P50–S7

MartosF

uentes

MarinaM

arta

UniversidadPolitécnicadeCartagena

marina.fuentes@

upct.es

P68–S8

Mascarenh

as

Lisette

SaskatchewanPulseCropDevelopmentBoard

lmascarenhas@

saskpulse.com

MavisNyo

hMowoh

SHUMASCAMEROON

sshumascameroon@yahoo.com

Maya-Blan

co

Valentin

CICYTEX-LaOrden,JuntadeExtremadura,Spain

valentin.m

aya@gobex.es

P31–S4

Mayerne

Nem

ere

Vera

Bonafarm

-BólyZrt.

Vera.M

ayerneNemere@Boly.bonafar

m.hu

McClean

Phillip

NorthDakotaStateUniversity

phil.mcclean@gmail.com

McPhe

eKevin

NorthDakotaStateUniversity

kevin.m

cphee@ndsu.edu

Oral–S17

Mecha

Elsa

ITQBNOVA

emecha@itqb.unl.pt

Oral–S9

Mustaph

aAdetayo

preservecollege

temtechnology@

gmail.com

Narits

Lea

EstonianCropResearchInstitute

lea.narits@etki.ee

P44–S7

Nayyar

Harsh

PanjabUniversity,Chandigarh,INDIA

nayarbot@

pu.ac.in

P16–S2

Nazzicari

nelson

CREA-CentreforFodderCropsandDairy

Productions

nelson.nazzicari@crea.gov.it

P30-S4

Nelson

Matthew

RoyalBotanicGardensKew

m.nelson@kew.org

Oral–S4

Ode

wun

mi

Adetola

KKSGLO

BALINVESTMENTNIGERIALIM

ITED

kksglobalinvestment@

yahoo.com

Oladipo

Solomon

solomonoladipoenterprise

oladipoakins@

yahoo.com

Oliveira

Miguel

UTADCentreforFunctionalEcology,Departmentof

LifeSciences,

miguelp.oliv@gmail.com

P13–S2

Oliveira

Rui

UniversityofCoim

bra,PolytechnicInstituteofPorto

rsoliveira@uc.pt

P171–S18

Olle

Margit

EstonianCropResearchInstitute

margit.olle@gmail.com

P92-S12

Oluwakayod

e

Arirejogun

UniversityofIbadan,Nigeria

adefisanadeniyi@

hotm

ail.com

Omon

dia

Emionwele

OmadConceptNigLim

ited

omadconcept@

yahoo.co.uk

Omri

Noura

INRATunisia

noura.m

ori@gmail.com

Ortiz

Rodomiro

SwedishUniversityofAgriculturalSciences

rodomiro.ortiz@slu.se

Osorno

Juan

NorthDakotaStateUniversity

juan.osorno@ndsu.edu

Oral–S18

Ouji

Ali

InstitutionofAgriculturalResearchandHigher

Education.NorthwestSemi-AridRegion,ElKef.

ali_ouji@yahoo.fr

Özkan

Hakan

ÇukurovaUniversity

hozkan@cu.edu.tr

P42–S4

Paiva

JorgeAlm

iro

InstituteofPlantGeneticsPolishAcademyof

Sciences

jpai@

igr.poznan.pl

P77–S8

Pakn

ejad

Farzad

KarajB

ranch,IslamicAzadUniversity,Karaj,Iran

farzadpaknejad@yahoo.com

P86–S11

Papa

Roberto

UniversitàPolitecnicadelleM

arche,Italy

r.papa@univpm.it

P75–S8

Parreira

José

ITQBNOVA

jsalvado@itqb.unl.pt

P70–S8

Pasche

Julie

NorthDakotaStateUniversity

julie.pasche@ndsu.edu

P172–S18

Pavan

Stefano

UniversityofBari

stefano.pavan@uniba.it

P175–S18

PazosN

avarro

Maria

UniversityofWesternAustralia

maria.pazosnavarro@uwa.edu.au

Oral–S13

Pecetti

Luciano

CREA-CenterforFodderCropsandDairy

Productions

luciano.pecetti@

crea.gov.it

Oral–S15

Pelzer

Elise

INRA

pelzer@

grignon.inra.fr

Oral–S14

Penm

etsa

R.Varm

a

UniversityofCaliforniaatDavis

rvpenmetsa@ucdavis.edu

P76–S8

Pereira

Graça

INIAV

graca.pereira@iniav.pt

P37–S4

Pérezd

elaVega

Marcelino

UniversidaddeLeón

m.perez.delavega@unileon.es

P144–S18

Peric

Vesna

MaizeResearchInstitute"ZemunPolje"

vesnaperic@

yahoo.com

P32–S4

Petrov

ić

Gordana

InstituteofFieldandVegetableCrops,NoviSad

gordana.petrovic@

nsseme.com

P159–S18

Petrov

ić

Kristina

InstituteofFieldandVegetableCrops

kristina.petrovic@

ifvcns.ns.ac.rs

P95-S12

Pilet-Nayel

Marie-Laure

INRA

marie-laure.pilet@

rennes.inra.fr

Oral–S6

Pilorgé

Etienne

TerresInovia

e.pilorge@terresinovia.fr

Polanco

Carlos

UniversidaddeLeón

carlos.polanco@unileon.es

P145–S18

Pooryo

usef

Mahmoud

Mahabadbranch,IslamicAzadUniversity

dr.pooryoosef@

gmail.com

P129–S14

Porque

ddu

Claudio

CNR-ISPAAM

c.porqueddu@cspm.ss.cnr.it

Oral–S14

Proskina

Liga

LatviaUniversityofAgriculture

liigaproskina@inbox.lv

Oral–S9

Prud

ent

Marion

INRA

Marion.Prudent@

dijon.inra.fr

P154–S18

Puspita

sari

Winda

Georg-August-UniversityofGoettingen

winda.puspita@gmail.com

P119–S13

Quillévéré-Ham

ard

Anne

INRA-IGEPP

anne.quillevere@rennes.inra.fr

P101–S12

Ravn

skov

Sabine

AarhusUniversity

Sabine.Ravnskov@

agro.au.dk

Rebo

laLichten

berg

Jessica

Georg-August-UniversityofGöttingen

jessica.rebola-lichtenberg@forst.uni-goettingen.de

P59–S7

Rewald

Boris

UniversityofNaturalResourcesandLifeSciences,Viennaboris.rewald@boku.ac.at

Oral–S15

Ribe

iro

Carlos

UniversidadedeTrásosMonteseAltoDouro

cribeiro@utad.pt

P27–S4

Rispail

Nicolas

InstitutodeAgriculturaSostenible-CSIC

nrispail@

ias.csic.es

P181–S18

Robles-Bolivar

Paula

CSIC,EstacionExperimentaldelZaidin;Department

ofBiochemistry,CellandM

olecularBiologyofPlants

paurrobo@gmail.com

P85–S11

Rocha

Inês

CentreforFunctionalEcology,DepartmentofLife

Sciences,UniversityofCoim

bra

ines.sousa.rocha@uc.pt

P17–S3

Rone

nGil

NRGene

gil@

nrgene.com

Rosa

Eduardo

UTAD

erosa@utad.pt

Rosewarne

Garry

AgricultureVictoria,Australia

garry.rosewarne@ecodev.vic.gov.au

P124–S13

Rubiales

Diego

CSIC,Cordoba,Spain

diego.rubiales@

ias.csic.es

Oral–S18

Rubio

Josefa

IFAPA

josefam.rubio@juntadeandalucia.es

Ruge-W

ehlin

gBrigitte

JuliusKuehn-Institut

brigitte.ruge-w

ehling@julius-kehn.de

Oral–S18

Ruland

Michael

Georg-August-UniversitätGöttingen

mruland@gwdg.de

P33–S4

Rusilowicz

Martin

FeraScienceLim

ited

mjr129@york.ac.uk

P169–S18

Rybiński

Wojciech

InstituteofPlantGeneticsPolishAcademyof

Sciences

wryb@igr.poznan.pl

P24–S4

Sakiroglu

Muhammet

KafkasUniversity

msakiroglu@kafkas.edu.tr

P69–S8

Salawu

Olakunle

Olarok&Co

olarokco@gmail.com

Sallaku

Glenda

AgriculturalU

niversityofTirana

gsallaku@ubt.edu.al

P128–S14

Salon

Christophe

Inra

christophe.salon@dijon.inra.fr

Sánche

zNavarro

Virginia

UniversidadPolitécnicadeCartagena

virginia.sanchez@

upct.es

Oral–S17

Santalla

Marta

CSIC

msantalla@mbg.csic.es

Oral–S10

Santos

Carla

CBQF-CentreforBiotecnologyandFineChemistryof

UniversidadeCatólicaPortuguesa

carla.sancho.santos@

gmail.com

P180–S18

Sari

Duygu

AkdenizUniversity

duygusari@akdeniz.edu.tr

P162–S18

Sarkar

Abhim

anyu

TheJohnInnesCentre,Norw

ich,U.K.

abhim

anyu.sarkar@

jic.ac.uk

P74–S8

Sarker

Ashutosh

ICARDA

a.sarker@

cgiar.org

Oral–S13

Sarpon

gBernard

RUGZILLTD

allenmorris0112@gmail.com

Savvas

Dim

itrios

AgriculturalU

niversityofAthens

dsavvas@

aua.gr

Oral–S17

Scegura

Amy

NorthDakotaStateUniversity

amy.scegura@ndsu.edu

P155–S18

Schn

eide

rAnne

TerresInovia

a.schneider@

terresinovia.com

P15–S2

Scrim

shaw

Jim

PGRO

jim@pgro.org

Seab

raPinto

Alexandra

INIAV

alexandra.pinto@iniav.pt

P1–S1

Seiden

glan

zMarek

AGRITECL.t.d.

seidenglanz@

agritec.cz

P102–S12

Seljåsen

Randi

NIBIONorw

egianInstituteofBioeconomyResearch

randi.seljasen@nibio.no

P135–S14

Senb

erga

Alise

LatviaUniversityofAgriculture

alisesenberga@gmail.com

P184–S3

Serran

oMariado

Carm

o

INIAV,I.P.

carm

o.serrano@iniav.pt

Oral–S9

Severin

sen

Annegrethe

PsykologPraksis

psykologen@mail.dk

Sharma

Mamta

ICRISAT

mamta.sharm

a@cgiar.org

P161–S18

Shtie

nberg

Dani

ARO,theVolcaniCenter

danish@volcani.agri.gov.il

Sieb

recht

Daniel

Georg-August-UniversitätGöttingen,Department

ofCropSiences,DivisionofPlantBreeding

daniel.siebrecht@

agr.uni-goettingen.de

P109–S13

Sivasank

ar

Sobhana

CGIARResearchProgramonDrylandCereals;CGIAR

ResearchProgramonGrainLegumes;ICRISAT

s.sivasankar@

cgiar.org

Sm

ykal

Petr

PalackyUniversityinOlomouc

petr.smykal@

upol.cz

Oral–S10

Sousa

Manuel

FrescuraSublim

eUnipessoalLda.

sousamaf@

gmail.com

P132–S14

Stod

dard

Fred

UniversityofHelsinki

frederick.stoddard@helsinki.fi

Stou

gaard

Jens

AarhusUniversity,Denmark

stougaard@mbg.au.dk

KeyLec–S3

Streit

Juliane

Georg-August-UniversityGoettingen|Department

forCropSciences|DevisionofAgronomy

juliane.streit@agr.uni-goettingen.de

Oral–S16

Sutton

Tim

SouthAustralianResearchandDevelopment

Institute

tim.sutton@sa.gov.au

Svab

ova

Lenka

AgritecPlantResearch,Ltd.

svabova@agritec.cz

P82–S8

Szab

óZoltan

NAIK

szabo.zoltan@abc.naik.hu

Tampa

kaki

Anastasia

AgriculturalU

niversityOfAthens

tampakaki@aua.gr

P28–S4

Taran

Bunyamin

UniversityofSaskatchewan

bunyamin.taran@usask.ca

Oral–S15

Tham

i-Alami

Imane

INRAM

orocco

thamialami_ma@yahoo.fr

P186–S3

Thom

pson

Richard

INRAUMRAgroecologie

thompson@dijon.inra.fr

Toba

rPinon

MariaGabriela

NorthDakotaStateUniversity

gabriela.tobarpinon@ndsu.edu

P35–S4

Toklu

Faruk

ÇukurovaUniversityY

fapet@

cu.edu.tr

P41–S4

Tollena

ere

Reece

INRA

reece.tollenaere@rennes.inra.fr

P174–S18

Toom

Merili

EstonianCropResearchInstitute

merili.toom@etki.ee

P182–S3

Torm

oElodie

TERRESUNIVIA

e.torm

o@terresunivia.fr

Oral–S9

Torres

AnaM

aria

IFAPA

anam.torres.romero@juntadeandalucia.es

Oral–S10

Toyo

da

Kazuhiro

OkayamaUniversity

pisatin@okayama-u.ac.jp

Oral–S18

Turkeri

Meltem

EastM

editerraneanAgriculturalResearchInstitute-

ADANA

irmakturk@hotm

ail.com

P191–S18

Upa

dhyaya

HariDeo

ICRISAT

h.upadhyaya@cgiar.org

Vågen

Ingunn

NIBIO-Norw

egianInstituteofBioeconomyResearch

ingunn.vaagen@nibio.no

VazP

atto

MariaCarlota

ITQBNOVA

cpatto@itqb.unl.pt

Oral–S10

Verm

aPraveen

NationalInstituteofPlantGenomeResearch

pkv@nipgr.ac.in

P83–S8

Vernou

dVanessa

INRA

vanessa.vernoud@dijon.inra.fr

P156–S18

Verret

Valentin

INRA

valentin.verret@

grignon.inra.fr

Oral–S17

Vickers

Roger

PGRO

Roger@

pgro.org

Vide

iraeCastro

Isabel

INIAV,I.P.

isabel.castro@iniav.pt

P188–S3

Villegas-Fernán

dez

Ángel

SpanishNationalResearchCouncil

amvf@cica.es

P94-S12

Vogt-Kau

te

Werner

NaturlandFachberatung

w.vogt-kaute@naturland-

beratung.de

P2–S1

Voisin

Anne-Sophie

INRADijon

anne-sophie.voisin@dijon.inra.fr

vonWettberg

Eric

FloridaInternationalU

niversity-MainCampus

eric.vonwettberg@fiu.edu

Oral–S4

Vorster

Juan

UniversityofPretoria

juan.vorster@

up.ac.za

Oral-S11

Vosatka

Miroslav

Symbiom

vosatka@ibot.cas.cz

Oral–S3

Vujic

Svetlana

UniversityofNoviSad,FacultyofAgriculture

antanasovic.svetlana@polj.uns.ac.rs

P5–S2

Vymyslicky

Tomas

AgriculturalResearch,Ltd.

vymyslicky@vupt.cz

P4–S2

Wan

Ping

BeijingUniversityofAgriculture

pingwan3@163.com

Oral–S8

Ward

Rebecca

ProcessorsandGrowersResearchorganisation

becky@

pgro.org

P93-S12

Warkentin

Tom

UniversityofSaskatchewan

tom.warkentin@usask.ca

Oral–S15

Watson

Christine

SLU/SRUC

christine.watson@slu.se

Oral–S2

Wiesel

Lea

ProcessorsandGrowersResearchOrganisation

lea@pgro.org

P142–S18

Winter

Peter

GenXProGmbH

pwinter@

genxpro.de

P160–S18

Witten

Stephanie

ThünenInstituteofOrganicFarm

ing-FederalRes.

InstituteforRuralA

reas,ForestryandFisheries

stephanie.witten@thuenen.de

P131–S14

Woo

dThomas

NIAB

tom.wood@niab.com

P177–S18

Yamasaki

Shiori

OkayamaUniversity

pdcx1ose@s.okayama-u.ac.jp

P166–S18

Yucel

Derya

AgriculturalM

inistry

deryayucel01@gmail.com

P141–S18

Zaidi

Farid

UniversityofBejaia,Algerie

idiazdiraf@

yahoo.fr

P64–S7

Zeipina

Solvita

PūreHortculturalResearchCentre

solvita.zeipina@gmail.com

P66–S7

Živano

vDalibor

IFVCNS/InstituteofFieldandVegetableCrops,Novi

Sad

dalibor.zivanov@

nsseme.com

P55-S7