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The use of thy-1-YFP-H mice in analysing peripheral nerve regeneration
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The use of thy-1-YFP-H mice in analysing peripheral nerve regeneration Adam Harding
Transcript of The use of thy-1-YFP-H mice in analysing peripheral nerve regeneration
The use of thy-1-YFP-H mice in analysing peripheral nerve regeneration
Adam Harding
Nerve injuries in oral surgery
Common following lower 3rd molar removal and sagittal split osteotomy.
Assessing experimental repairs
Electrophysiology.Gait/walking analysis.Fibre counts.
thy-1-YFP-H mice
Genetically modified to express yellow fluorescent protein in a subset of nerve cells.
Experiment Setup
Common fibular nerve sectioned and repaired with graft or nerve guide.
Analysis Methods
Counting axons at points along repair.
Tracing paths taken by nerve fibres.
Mannose-6-phosphate study
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Nerve guide study 1
Nerve guide study 2
Issues
Labour intensive/subjective analysis.
Expensive mice – breeding colony required.
Fluorescence obstructed:
Benefits
Provided possible explanation for previous results.
Revealed information that would not have been possible using other methods.
Short time period between setup and results.
Acknowledgements
MRC.Renovo.Prof. Fiona Boissonade.
Prof. Alison Loescher.
Prof. John Haycock.
Prof. Neil Hopkinson.
Dr. Claire Christmas.
Dr. Fred ClaeyssensChristopher Pateman
