l

ACADEMY OF SCIENCES OF THE UKRAINIAN SSR

DEPARTMENT

OF BIOCHEMISTRY. PHYSIOLOGY AND THEORETICAL MEDICINE

BIOPOMR,SA]\TD CELL aALL-UNIONSCIEN IFIC -THEORETICAL

JOURNALJULY-AI.'GUST

ESTABLISHED IN JA\UAR\' I9E5 APPLARS BIMONTHLY KIEV N,.\UKOVA DUA,IKA

voLUMET "4 1991

CONTENTS

Cell Biology

KIRI.HENK. I '

GIEPA 'u.

)-u. Individual. cu.lruring of preserectcd prantProtoplasts and Their il[; pioar.i. 'in"',r,ii.rrdropl.t. .

PASTERNAK T. p. Genetic Transforrnaiion of cerears: Advances and perspectivesKOSTENYUK I. A. LUBARETZ O. F., VORONIN.V. V., CLEBA yu. yu. CeliEngineering Dcveloped ti, A)ii, uriir*'pLii' BiotechnotogyKOLESNIK N. N.. KOMARNITSKY-I.-K., SHLUMII.KOY T-. N., SAMOYLOV A. S.Analvsis of Mitoiic Segregation

"i prr!*re.nrs ir wiiittiii iim-atic Hybridsobtained by Cloning ,i ii*.r,jilii.;.''F;r:5; products :

. .

KOVLER L. A., SHLUITUKOV L. \., CLEBA yu. yu. Thylakoid polypeptides Ana-lysis of Intertribal Cytoplismic '

Hyb-.id"r" '.CHEREP N. N., KOMARNITSKy I. K. Miiochonclrial Genome of Genus NicotianaGEVORKIAN R. L. SPANGENBERG H, CLEBA Iu, Ir. Reconstituied NicotiancForms obtained by curturi"g

"lFi"'i"piist"ic"vtoptast Fusion productsKUCHUK N. V.. SHAKqO\igrir A. M., VORONTN V. V, GLEBA yu. yu rsola-tion of Asymmerric Somuiic HyLr;1. ii'uli;is" Species ' :--.-...." .

lJr",:T,\ff-JvIELNICHUK G. c., FELALTEV A. S. /n Vitro rnduced Morphoge.

6

20

39

45

'"lY?i S,"fail't:,o":i1-,1, ?; J,.,ff?y.'I? ' u, M^KHoR;N^ O. K. Th,r

5I

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ZUBKO M. K. ZLIBKO E -]., XAPRANOV ph. V. Inductiorr ofcient Tobacco Mutants ,s mrriui, 'f"i"d"if 'Enginee.ing72

.., . .".".r i-i:rva..fq:y.:S,{i4*F**#";'t: 'I

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ll. Vasil I. K., Vasil V., Redaag F. Plant regeneration from embryogenic protoplasts of !Triticum aesttuum L. (wheat) ll7-lh lnt. congr. on plant tislue and'cell culture: ll

Abstrs.-Amsterdam, 1990.-P. 3. l:

12. Tilton V. $.., Russell S. H. Microinjection of plant cells. II. Cell isolation, prepara-tion and initial culture l/ Plant Physibl.- 1983.- 12, N l.- p. 9.

13. Ilacrepaarc T. II., Me.ttonweoe Z. B. lejnerntrecKar rpaHcQopuauun BHcrusx pacrennric noMoulblo Ml{I(poHH6eK(sfi AHK //Euotorun KynbrxBnpyeMbrx }c{eroK n 6uorexHo-Jrorlrn : Marepra.lu Mex(AyHap. xoHQ.- Honocll6npcr, 1988.- C. 187-188.

14. TransSenic rapsed plants obiained by the microinjection of DNA into nricrospore-de-rived embryoids / G. Neuhaus, G. Spadenberg, O. Mittelsiein-Scheid, H.-G. Schweiger i/Theor. and Appl. Genet.-1987.-75, N l.-P.30--36.

15. Vlei M, Kgo M., Harada H. Callus formation and plant regeneration through directculture of isoiated pollen grains of H. r:ulga7e,' gs. Sabarlis l Ibid.- 1986.- 72,N 2.- P. 252-255.

16. Transient expression of chloramphenicolacetyltransferase (CAT) gene in barley cellcultures and immature embryos through microprojective bombardment / K. K. Kartha,R. N. Chibbar. F. Georges el al. llPlant Cell. Rep.- 1989.-8, N 3.-P. 4n432.

i7. Plant cell transformation using high velocity microparticles/ R. H Vallejos, M. Lo-pez, L. Orsaria et al./17-th Int. cong. on plant tissue and ccll culture: Abstrs.-Amsterdam, 1990.- P. 80.

18. Transf ormation of cereal cell cultures by microprojective bombaidment/Chen D.,Batty N., Evans J., P. Dale lllbid.- P. 50.

19. Gene transfer to barley/R. R. Mendel, E. Claus, J. Schulze et al. lllbid.-P. 44.20. Kaprem H. A. 3QQexru sK3oreHuofi ,D.HK y Bbrcrxrlx pacreunii.-MxHcx:Hayxa N

rexHnxa, 1981.- 143 c.21. Transfer of bacterial and human genes to germinating Arabtdopsis thalianalL. Le-

doux, L. Diels, R. Huart et al.//Arabidopsis inform. service.- 1985.-22, P. l-12.22. Uptake and transient expression of chimeiic genes in seed-derived embryosi R. Top-

fer, B. Gronenborn, J. Schell, H. Steinbiss llPlant Cell.- 1989.- I, N l.- P. 133-I 39.

23. De la Pena A., Lorz H., Schell l. Transgenic rye plants obiained b,v injection of DNAinto young tillers // Nature.- 1987.-325, N 6071.- P. 274-276.

24. Hess'D. Pollen-based techniques in genetic manipulation ll lnl. Rev. Cytology.-1987.- 107.- P. 367-395.

25. De Vet I. M., Bergqust R., Harlan ./. Exogenous gene transfer in maize using DNA-treated pollen / Exp. manipulation of ovule tissues / Ed. P. Charman.- New York,1985.- P. 197-209.

%. Ohta l| High efficiency genetic transiormation of maize by a mixture of pollen andexosenous DNA//Proc. Nat. Acad. Sci. USA.- 1986.-83, N 3.-P. 715-719.

27. Vechorcoe IO. 8., Burconcrcan H. A., Kopo'I; A. E. leNeruqecxag rpaucQopr,,rauut K-v-

Kvpy3br ll 7-Fr Bcecoto3. cuM[o3. <Morery;rrpubte MexaHH3MH reHerHqecKnx npouec-c6ii : Teg. AoK.'I.- M., 19€0.- C. 183.

28. Boog G., Krens F., Huizing H. Attempted pollen mediaied transformation of maize llJ. Piant Phvsiol.- 1989.- t35, N 3.- P. 319-324.

N. Luo 2., lvi R. A simple method for the transformation of rice tia'the pollen tubepathvray llPlant Mol. Biol. Rep.- 1988.-6, N 3.- P. 165-174.

30-'Pacreniti tqMenq c BBeaeHHbIM reHo\I KaHallHl{}lHycrofiquBocr}i I H. A. Kapreas'K. H. 3a6enrxoea, T. B. ManeruuHa, C. E. A6,roe ll &oxtl AH bCCP-- 1990'-34'N9 3.- C. 261-263.

31. Flaoell R., Mathias fi. Prospects for1984.-307, N 5947.-P. 108-109.

transforming monocot crop plants // Nature.-

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CIIHCOK,NI.{TEPATYPbI

I Yamada l'.' Hasltimolo.J' Possibilities for itttproving. yicltJs of secorrdarl rrrctabolircsin plant ccll culturcs ll.Progr..in plant ccll arirl mol]biol.: proc -i,il Int. consr. olantrissue rrirt teil cult. (Amsierdam, 24-29 Jurrc__l-990) / Eds ri. .r j'iljli;;";i"ri:Dordrecht : Kluwer Acad. publ., 1990._ p. b47_Sb6.

2. Eunice A., Foaler ff.,Biologi^cally active_plant-secondary metabolites-perspectivesf or the f uture // Chem. Ind -1985.- lZ .- p'. 40g-410.

3- Fontanel A., Tabata M. Produciion of secondary metabolites by plant tissue and cellcultures. Present aspects and prospects // Nestle Res. News id6d6i.--ir...;), ,'irl-.rt.;Ltd., 1987.- p. 93-1034- ){uano pacrerrufi. I-lserxonHe pacreHlfl.- M.: flpocneuterrue, l98l.- T. b, q. 2._512 c.5. cnpaaouxux no .nexap.crneurlulu pacreHnnv-/ h. M.' 3aiopoxur,.i, Al r.- Kor"ux,

C. fl. Coxoaos H Ap.- M. : .IIecH. npor,,l-crt, lggg.- 415 c.6. Pawelha K-H., Stochigl /. Major'indole alkaloids in cell suspension cultures ol Rha-zga stricta Decaisne llZ. Nalurforsch.- 1986.- 4lC._ p. 3g5_390-7. Balseuich.I. I{onoterpene indole alkaloids lrom Apocgnirio, oli"t lhen Catharanthus

raseus llcell culture and somatic_cell_ genetics of 'pla-nts/Eds

F. constabel, I. K. rra.sil.- San Diego : Acad. press, 198g.- Vol. 5.- p. g7l_3g5.8- DeLuca V., Kurz V(. G. V. Monoierpene indole alkaloids (Catharanllrzs alkaloids) //Ibid.- P. 385--401.9. Cudopct6 B. ,4. EnorexHoJ'lorufl pacrcuuti. K;reroqHaq ce,reKrlHrr.- Klres : Havx. AyMKa,

I 990.- 280 c.10. Bapat V., A-, Rao P. 5., IIeble If. R. Lnmobilization of cells and orotoolasts oi

catharanthus roseus llProc. Indian Acad. Sci.- 1986.-96, N 5.- p. drs-iie. -- -'

ll. Taheuchi Y., Komani.ne l. Glucans in the cell rvalls regenerated fronr l/itca roseap_rotoplasts llPlanl Cell Physiol.- 1981.- 22, N lt.- p. 1585_1591.

12. co*t_aruqecxan rn6pugvtar{}rr nacneHoBux / B. A. cr,r,opon, H. M. llueeHr, }o. lo. f,re-6a, K. M. CurHnx.- Kuen: Hayx. .rIyMKa, 1985.- 130 c.

13. Medgge.sg P., Me-nczel L., Maliga P. The use oi cytoplasmic strcptoml,cin resistance:clrloroplast iransfer |rom Nicotiana tabacum into Nicotiana sgla'eslrii, and isolationof their somatic hybrid//Mol. and Gen. Genet.- 1980.- lZ9,-N 3.-p. 693--698

14. Euoxunu,aecxui auaaut B KreroqHoft llnxeHeprlH pacreuufi.-Krren, 1988.- 4g c.-(llpenprHr/AH yCCP. Hu-r 6oraHuxu; N! 88.i).

15- Shure M., lYlessler 5., Fedoroff ly'. Molecular indentification and isolation of theWaxy locus in maize llCell.- 1963.-35, N 3.- P.225-233.

16. Ko/,owa B. O., Qodop H. ll. C.rpyxrypHar rereporer{Hocrr pAHK Citrus linonllMo-nexynrp. 6nonorus.- 1986.- 20, Ne 3.- C. 656-659.

17. Caboche M. Nutritional requirements of protoplast-derived haploid tobacco cell grownat low densities in liquid medium llPlanta.- 1980.- I49, N- l.- P. 7-18.

18. KarilHuH cb. JI., Capnaqran B. B_., Ilottur4grc B. E. Meroau Kynbr,vpbr rxaHeri n Quano-J'rorurr H 6rroxsvan pacreHuii.- Kaen: Hayx. ,u,yMKa, 1980.- 487 c. i

19. Menczel 1., .(iss Z. R., Maliga P. Streptomycin resistant and sensitive sonratic fir'b-rids of lrticotiana labacumlNicotiana hnightiana:Correlation of resist.ance to A'. /a-bacum plastids // Theor. and Appl. Genet.- 1981.- 59, N 2.- P. l9l-t95.

20. Borisguh \'. 1/., Momot l/. P., Gleba Y. Novcl class oi rD\.\ repcat units in sorrratichybrids bctu een Nicottana and Atropa ll lbid.- 1988.- 76, N l- P. 108-l 12.

21. Larkin P. t., Scoucrolt lY. R. Somaclonal variation. A novel source of variabilityfrom cell cultures ll lbid.- l98l - 60, N 2.- P. 197-214..

22. Valler G. R., Noaacki E. K. Genetic control of alkaloid production // Alkaloid Biol.and Metabol. in Plants/Eds G. R. Waller, E. K. Novacki-Nerv York; London:Ple-num press, 1978- P. 49-84.

Hrr-rKnes

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vaK 575 1

H. I{. Itoaccrrrri, lI. K. Konrapunqnuir, JI. P. IllayuyxoB, A. C. Cauoii.rroa

AHAJIIIS MI4TOTI4T{ECLOfi CE|PE|AIIITI4 IIJTA3MATEHOBB COMATUT{ECIIIIX TLIEPI,IAAX NICOTI ANA,IIOIYqEHIIbIX ITJIOHIIPOBAIII{EI\{IBTEPOIIJIASMATI4TIECKTIX IPOAyKTOB CJIr{flHr{f

Hsgwao nacttedoeanue wecru Kodupgeilbrx nna3nazevailu npuarratoa g eu1puduotx QopinNicotiana, peeeHepupolatrflbrx. u3 eu6pu0nwx KreroK neeaeucunoeo npoucxotcdenux.

-

B xa4ecr(te podurenocrux Qop.r,t ucnoto3ola.tu Ka,/tlrgcHbae nporonJtacTbt n.nacTottlo-29 ynopo$uta)eQercrtoeo ilgraHTa N. tabacum u neaoQinaue nporonnacru odvoeo u3

O H. H. Koaecsnx, H. K. Koxapnxqxxfi,.II. P- Illayuyxoa, A. C. CaMo[roB,1991.

34 ISSN 0233-7657- EHONOJIHMEPbI H KJIETKA. I99I. T. 7. }& +