Life science

813 l U.S. 800-323-4340 toll-free, 847-549-7600 (outside the U.S.), www.coleparmer.com Canada 800-363-5900 l India 91-22-6716-2222 l UK 0500-345-300

813

Table-of-ContentsWebRegistration

LI

Table of ContentsMolecular BiologyChilling heating blocks .................839DNA centrifugal concentrator system ...........................................817DNA and RNA decontaminants ............................838HybridizationChemiluminescent kit .........824–825Crosslinkers .................................842Incubators ............................840–842

MicroArray slides and plates .......833PCRCloning disc .................................838Enzyme removal kit .....................828PCR cleanup kit............................828Reagents ......................................837Seal films for PCR .......................838Thermal cyclers ...................834–835Tube and plates ...........................836

Protein ChemistryCentrifugal protein concentrators ...............................830Centrifugal devices .....................829Dialysis products .................369–373Protein assay kits ................825–826Protein purification .....................827

Scintillation and luminescence counters .................................843–844

ImmunologyAntibodies ..... www.coleparmer.comAssay plates ..........................818–821Colorimeter ...................................817ELISA substrates ...........................823Microplate washer and readers ...................................814–816Seal films for microplates ....822–823

Tissue and Cell CultureAseptic sampling ..................865–866Bio-bags, BioProcess containers ..............................862–864Bioprocess tanks ...........................862Bioreactors ....................................861Chamber slide systems ................851Culture plates and flasks ......................................845–850Culture tubes .................................853Culture vessels and system ...................................857–859Culture waste disposal system .....856Hemacytometers ...........................854Media bottle ..................................855Roller culture apparatus .......860–861Tissue grinder ...............................854Tubing for bioprocessing systems ..........................................863

Life Science Related SectionsAnimal Physiology ....................49–53Aseptic sampling ..................865–866Bath and Circulators .............104–133Centrifuges ............................204–225Chromatography ...................227–248Cryogenics .............................334–339Dialysis ..................................369–373Electrophoresis .....................401–413Electroporation .....................414–416Fermentation .........................437–444Filtration, Laboratory ............445–470Freeze Dryers ........................646–648Histology ...............................696–700Homogenizers .......................701–710Hoods .....................................711–731Incubators ..............................770–781Microbiology .........................902–922Microfluidics .............. 923–934Microscopes ..........................935–962Pipettes and Pipettors ......1134–1163Shakers ..............................1539–1554Spectroscopy ....................1582–1593Sterilizers ...........................1602–1608Stirrers ...............................1609–1628Syringe Pumps ..................1383–1387UV Equipment ...................1902–1913Water Purification .............1989–2001

Look for these new, innovative products!

Multiwash III plate washers

(page 814)

Immobilized protein A, G, A/G, and L (page 827)

Disposable hemacytometers (page 854)

Gradient thermal cyclers

(page 835)

Nano syringe pump (page 1384)

Imaging systems

(page 411)

Micro-flow piston pumps

(page 926)

Digital shaking incubators (page 775)

HotStart® PCR

reaction tubes

(page 837)

Chilling/ heating/mixing

blocks (page 839)

ProCulture®

spinner flasks (page 859)

BioLite™ MultiSpectral source

(page 1907)

ColeParmer.com/LifeSciences to view our latest products.

Aseptic sampler system

(page 865)

Life ScienceTable of Contents

l U.S. 800-323-4340 toll-free, 847-549-7600 (outside the U.S.), www.coleparmer.com Canada 800-363-5900 l India 91-22-6716-2222 l UK 0500-345-300

814


ImmunologyLI Life Science

Easy to use—connect to a vacuum source and washing solution. Place cannulae in wells, then press knob to dispense wash solution. Release, and vacuum aspirates liquid. End tabs prevent cannula from touching well surface and interfering with assay. Autoclavable and acid resistant.

What‘s included: storage rack and a spare parts kit.

Immuno™ WashersWash 96- and 384-well microplates and stripsl Specially designed coaxial cannulae eliminates overfilling

and cross-contamination

Specifications & Ordering Information

Catalog number Number of channels For plate size PriceKH-01929-25 8 96-well KH-01929-26 12 96-well KH-01929-27 16 384-well 01929-26

Multiwash III Plate Washersl Quick-connect fittings allow unit to be fully operational in 15 minutesl Rinse-on-demand feature for complete system cleaning at the touch of a buttonl Can handle full range of assay types from the most gentle wash to the most

aggressive ELISAThese plate washers feature easy-to-program wash routines, bottom washing, sweeping crosswise aspiration, dispense only and aspirate only functions, and overflow washing for continual dispensing while aspirating off the top for a thorough wash. Use the plate washer to perform cell washing; agglutination tests; cytotoxicity tests; and ELISA, EIA, FIA, and LIA tests.

Minimum residual volume is ≤1 µL per well. Washer has seven programmable wash operations. Independent adjustments of horizontal and vertical needle positions allow any shape well to be accommodated; extra aspiration adjustment for both horizontal and vertical needle positions can be stored in individual programs. Wetted materials include glass, nylon, PTFE, stainless steel, polypropylene, Tygon®, Delrin®, polyethylene, and Santoprene®.

The washer performs a 300-µL three-cycle wash on a full 96-well plate in less than two minutes. You control the dispense pressure. By programming and storing routines, you can switch between seven different modes at the touch of a button. The washer also features a floating manifold that adjusts to match each plate and allows the washer to perform special test procedures. All units are CE-approved, ETL-listed, and certified with CAN/CSA Std C22.2, No. 1010.1.

What's included: manifold, slip connectors, reservoir kit, two power cords (U.S. standard and European), fuse, rinse strips, dust cover, and, quick-start guide. Order additional manifolds separately below.

Full plate processing time 8-port manifold: 180 seconds 12-port manifold: 120 seconds 16-port manifold: 90 seconds

Accuracy: ±3% at 300 µLPrecision: ≤3% CV at 300 µLMinimum residual volume: ≤1% per wellDispense volume per well

8-port manifold: 50 µL to 1.99 mL 12-port manifold: 50 µL to 1.65 mL 16-port manifold: 50 µL to 1.25 mL

Programs: 50 preprogrammedMaximum numbers of cycles: tenWash and rinse reservoir volume: two liters eachWell shape: independent horizontal and vertical

adjustments allow any shape well to be accommodated

Soak time: 0 to 999 secondsDimensions: 11" W x 81⁄2" H x 141⁄2"DPower: 90 to 126 VAC/210 to 258 VAC, 50/60 Hz

Catalog number

Manifold included Type Manifold

material Price

KH-13054-34 No — — KH-13054-02 Yes 8-port Standard KH-13054-10 Yes 12-port Standard KH-13054-12 Yes 16-port Standard KH-13054-14 Yes 8-port Coaxial KH-13054-16 Yes 8-port Coaxial KH-13054-18 Yes 16-port Coaxial


Catalog number Type Manifold

material Price

KH-13054-20 8-port Standard KH-13054-22 12-port Standard KH-13054-24 16-port Standard KH-13054-26 8-port Coaxial KH-13054-28 12-port Coaxial KH-13054-30 16-port Coaxial

Interchangeable manifolds

AccessoriesKH-13054-54 Aerosol shield

13054-34

ColeParmer.com/ImmunologySearch online for all our new items— product lines are continually updated.


815


ImmunologyLILife Science

Wash method includes shaking, soaking and bottom washing options—up to 10 cycles l Washes single 1" x 8" or 1" x 12" strips or full 96-well platesl Self-contained and programmablel For EIA, fluorescence, chemiluminescence, RIA, DNA probe,

and cellular assaysThese washers feature a micro-controlled, syringe-drive, positive- displacement pump for fluid delivery and variable control of aspiration rates. Intuitive, menu-driven software lets you easily automate washing protocols, even those specialized for stringent research applications.

Programmable aspiration rates reduce carryover and ensure low background absorbance and small residual volumes. Preprogrammed maintenance protocols are available at the touch of a button, and automatic buffer switching is optional on all models. Safety features include aerosol cover, built-in decontamination routine, and isolation of electronics from fluids. For in vitro diagnostic use only.

What’s included: one set of 8- or 12-channel manifold; supply tubing set; wash, rinse, and waste 2 L bottles; aerosol cover; vacuum filter; and maintenance kit. Order additional manifold separately.

Automatic Strip Washers

AccessoriesKH-13055-10 Eight-channel manifoldKH-13055-12 Twelve-channel manifold

Optional 72/96 Terasaki and 384-well capacity, UV 340 nm capabilityl Stores up to 75 custom assay definitions and eight test resultsl Extensive on-board data analysis and optional software offer

more flexible data manipulationThese microplate readers feature on-board data reduction and reporting. They read 6-, 12-, 24-, 48-, and 96-well plate microplates. Model 13055-52 can read 384-well and 60-, 72-, and 96-well Terasaki plates. Wavelength ranges from 400 to 750 nm. Model 13055-54 optics extend wavelength range down to 340 nm.

Microplate readers feature a 2 x 24-character LCD and a convenient soft keypad. Extensive curve fitting options include linear, cubic, quadratic, 4-p, log-logit, cubicspline, and point-to-point. Memory stores 25 standard curves and up to eight microplate test results. Readers feature auto or manual plate layout for blanks, controls, standards, and samples. They also offer control and assay validation, transformation and formula applications, and cutoff and call criteria.

On-board software is easy to program to run sophisticated assays and analyze data without a computer. Additional software 13055-56 offers easy user interface with flexible data manipulation and curve fitting for basic endpoint, kinetic, multi-wavelength and spectral scan requirements. Software package includes software on CD, and three user licenses.

What’s included: 340 (13055-54 only), 405, 450, 490, 630 nm filters, dust cover, parallel printer cable (25-pin male and 36-pin male), and serial cable (null modem, 9-pin female to 25-pin female). Additional filters are available with ranges from 304 to 750 nm; call our Application Specialists or visit our Web site for more information.

Universal Microplate Readers


Catalognumber

Wavelength range (nm)

Microplatecapability Power Price

KH-13055-50 400 to 750 6-, 12-, 24-, 48-, 96-well100/240 VAC,

50/60Hz

KH-13055-52 400 to 750 6-, 12-, 24-, 48-, 72/96 Terasaki, 96- and 384-well

KH-13055-54 340 to 750 6-, 12-, 24-, 48-, 96-well

13055-50

Absorbance measurement range: 0 to 3.0 OD

Accuracy: <1% at 2.0 ODLinearity: 1% from 0 to 2.0 ODLight source: tungsten halogen bulb

Minimum read speed: 96-wells in 30 seconds

Dimension: 15"W x 7"W x 161⁄2"DFilter arrangement: five-cavity wheel

13055-00

Specifications & Ordering InformationManifold type: 8- or 12-channel Residual volume: <4 mL/wellMinimum wash speed: <130 seconds (3 cycles, 300 mL/well, 96 wells)Dispense volume: 25 to 3000 mL/wellWash cycles: 1 to 10 Buffer selection: valve module option—up to three buffersSoak time: 1 to 600 secondsFunctions: wash, prime, dispense, aspirate, rinse, maintenance routinesDimensions: 16"L x 14"W x 61⁄2"H

Catalog number Description Power PriceKH-13055-00 8-well strip washer 100/250 VAC, 50/60 Hz KH-13055-02 12-well strip washer

Call our Application Specialists for more information on respirators.

847-549-7600

AccessoriesKH-13055-56 Plate reader software


816



SpecificationsDetectors: photon counting photomultiplier and photo diodeOptical type: direct illumination (fiberless optics)Light source: xenon flash lamp-closed loopFluorescence sensitivity

Fluorescence intensity (FI): <2 fmol Fluorescence polarization (FP): 5 mp Time-resolved fluorometry (TRF): <100 amol Eu Excitation: 230 to 620 nm Emission: 360 to 630 nm (700 nm optional) Dynamic range: >5 decades

Luminescence sensitivity: 1 amol ATP/well, >6 decades dynamic rangeLiquid scintillation

Counting: 30% for 3H in a 96-well plate Efficiency: 50% for 3H, 50% for 125I and 80% for 14C in a 96-well plate

Absorbance Linearity: 2% Dynamic range: 3.0 OD Wavelength range: 230 to 900 nm

Output: RS-232 Power: 110 to 240 VAC, 50/60 HzDimensions: 151⁄2"L x 141⁄2"W x 113⁄4"H

Up to five technologies in one unit!l Read absorbance, luminescence, liquid scintillation,

fluorescence intensity (FI), and time resolved fluorescence (TRF) l Designed to read 24-, 96- and 384-well platesThese microplate readers are compact in size and save valuable bench space. An internal microprocessor with easy-to-use software PC including data export to Excel™. All models can have additional functions added at a later time.

Fluorescence Reader is ideal for both short life-time fluorophores and long life-time fluorescence using Fluorescence Intensity (FI), and Time-Resolved Fluorescence (TRF) modes. Applications include DNA hybridization studies, binding studies, enzyme assays, ligand receptor binding assays, cytotoxicity and immunoassays.

Absorbance Reader has a wavelength range from 230 to 900 nm. Typical applications include DNA quantification, colorimetric assays and enzyme assays.

Luminometer measures both “glow” and “flash” type reagents. Use for ATP detection, reporter gene assays, luminescence immunoassays, and toxicology. Flash luminescence function requires an injector (sold separately below).

Liquid Scintillation Counter has preset modes for the most commonly used radioisotopes (3H, 14C, 35S, 33P and 125I). Use for scintillation proximity assays (SPA), flash plate assays, cell proliferation studies, radioimmunoassays (RIA) and immunoradiometric assays (IRMA).

For further data analysis, order optional advanced microplate analysis software which includes curve fitting programs, statistical analysis of sample result calculations, detailed kinetic analysis, report generation templates, and Excel™-compatible export files.

13094-00

Chameleon™ Multi-Label Microplate Readers

Fluorescent Reference StandardsThese inorganic fluorescent reference standards are offered in a convenient 96-well microtiter plate format. This allows calibration of assay instrumentation such as fluorescent plate readers and DNA sequence detectors, providing a durable fluorescent standard.

Catalog number Color PriceKH-13094-60 Green KH-13094-62 Red

AccessoriesKH-13094-50 Injector for luminescence function. Up to two can be added KH-13094-52 Advanced microplate analysis software with interface

Catalog number Functions PriceTwo-function microplate reader

KH-13094-08 Liquid scintillation and luminescence Three-function microplate readers

KH-13094-12 Absorbance, luminescence, and liquid scintillation

KH-13094-18 Time resolved fluorescence, fluorescenceintensity, and liquid scintillation

Four-function microplate reader

KH-13094-20 Time resolved fluorescence, fluorescence intensity, absorbance, and luminescence

Five-function microplate reader

KH-13094-22 Time resolved fluorescence, fluorescence intensity,absorbance, luminescence, and liquid scintillation

Ordering Information

ColeParmer.com/LifeScienceEven MORE online! Look for new items, stock status, technical info, etc.


817



Full-Range ColorimeterFlexible tool for wide variety of analysesl Excellent for education and basic colorimetry applicationsl Easy output of reaction kinetics This versatile colorimeter is designed for easy wavelength adjustments to span common visible spectrum applications. Eight-filter wheel turns to exhibit desired color-coded wavelength—440, 470, 490, 520, 550, 580, 590, or 680 nm. Results can be displayed as absorbance units or % transmission. Use the kinetics mode to study rates of reaction and enzyme kinetics—outputs one reading per second.

Measure samples in either standard 10-mm pathlength cuvettes (regular or semi-micro) or in 16-mm diameter test tubes. Large digital display and simple push-button controls. Designed to withstand rugged use and clean up easily. Individual filters are replaceable. Analog and RS-232 outputs enable data transmission to PC, printer, or recorder.

Both models accommodate power sources of 110 to 250 VAC. Model 39459-62 also includes a built-in NiMH rechargeable battery, enabling on-the-spot analysis in any location.

Catalog number KH-39459-60 KH-39459-62Operational modes Absorbance, transmission, kineticsPhotometric range Absorbance: –0.3 to 1.99A; % transmission: 0 to 199%TWavelength range Standard gelatin filters: 440, 470, 490, 520, 550, 580, 590, 680 nmBandwidth 40 nmWavelength accuracy ±2.5 nmRepeatability ±0.02 at 1A using cuvettesOutputs RS-232, 0 to 2 VDimensions 6"W x 21⁄2"H x 7"DPower 110 to 250 VAC, 50/60 Hz 110 to 250 VAC, 50/60 Hz or rechargable batteryPrice


39459-60

Specifications & Ordering InformationTemperature range: ambient to 100°C in 1°C incrementsVacuum pump capacity: 34 L/min at 29.6"HgSpeed: 1725 rpmDimensions: 147⁄8"W x 115⁄8"H x 261⁄8"D

CentriVap® DNA Centrifugal Concentrator SystemDesigned to process DNA samples and small solvent samples— includes everything you needl Rapid evaporation—built-in 300 W heater warms samples during

concentrationl Separate timers control heat and run timesl Store multiple programs to retrieve later—view on digital display The centrifugal concentrator uses centrifugal force and vacuum to quickly evaporate solvents from multiple samples. The included rotor holds 60 x 0.5-mL or 72 x 1.5-mL microcentrifuge tubes. Automatic vacuum bleed valve releases vacuum when rotor stops to prevent loss of sample.

The acrylic lid is removable for decontamination; rubber seal ensures vacuum. The integral vacuum pump can be removed to use with other equipment.

What’s included: concentrator with integral vacuum pump, DNA rotor, inlet glass trap, and exhaust glass trap.

Catalog number Description Power (VAC,Hz) PriceKH-13040-10 Centrifugal concentrator system 115, 50/60 KH-13040-12 220, 50/60

AccessoriesKH-13040-50 Chemical trap, polypropylene. Provides additional protection to pump. Requires 13040-52 moisture insert or 13040-54 solvent insert KH-13040-52 Moisture insert for 13040-50 chemical trap KH-13040-54 Solvent insert for 13040-50 chemical trap

13040-10


818




96-Well PlatesPolystyrene plates meet the SBS standard to ensure compatibility with microplate and automation equipmentl Choose from three well shapes: flat wells maximize light transmission; round wells

optimize coating and washing; V-bottom wells let you completely retrieve well contentsl Clear-bottom plates have a unique thin polystyrene bottom—permit direct microscopic

viewing and CCD camera-based detections

Solid plates

Clear-bottom plates

Acetate Sealing TapeKH-01730-00 Acetate sealing tape for 96- and 384-well plates. Nonsterile. Case of 100

96-Well Microplates, ImmunologyFour surfaces with different degree of hydrophilicity, ideal for solid phase immunoassays (ELISA)l Engineered for easy/reproducible passive absorptionl Certified homogeneity of binding

The PolySorp surface is hydrophobic for binding biomolecules that have hydrophobic properties. MediSorp is slightly hydrophilic and binds proteins with a mixed hydrophilic/hydrophobic composition. This special surface provides an ideal balance of high protein absorption for coating yet low assay nonspecific binding. MaxiSorp is still more hydrophilic. It is optimized for binding immunoglobulin but also binds a wide range of other proteins. MultiSorp is the most hydrophilic surface offered. It binds biomolecules with a hydrophilic composition such a glycoproteins and glycans. Standard 96-well format (86 x 128 mm). Constructed of polystyrene.

Catalognumber

Well shape

Well vol (µL) Surface Surface area

(cm2/well) Application Qty/cs

Price/cs

Standard platesKH-01928-32 Flat 400 PolySorp 2.7 Hydrophobic 60 KH-01928-14 C-bottom 350 2.5 60 KH-01928-22 Flat 400 MediSorp 2.7 Hydrophobic/hydrophilic 60 KH-01928-10‡ Flat 400

MaxiSorp

2.7

More hydrophilic

60 KH-01928-11 Flat 400 2.7 60 KH-01928-13‡ C-bottom 350 2.5 60 KH-01928-30 C-bottom 350 2.5 60 KH-01928-23 Flat 400 MultiSorp 2.7 Most hydrophilic 60 LidsKH-01929-10 Nonsterile, with condensation rings, clear PS

Sterile, with condensation rings, clear PS100

KH-01929-11 50

01928-30

01928-32

01929-10


Catalognumber

Platecolor

Surfacetreatment

Wellshape

Wellvolume Sterile Lid Qty/

sleeveQty/cs

Price/cs

Solid platesKH-01728-07

Clear

Nontreated Round 360 µL Yes Yes 20 100 KH-01728-01 Nontreated Flat 360 µL Yes Yes 20 100 KH-01728-05 V-bottom 320 µL No No 25 100 KH-01728-22 High binding Round 360 µL No No 25 100 KH-01728-28* Flat 100 KH-01959-26†

Cell culture Flat 360 µL Yes Yes 5 100 KH-01959-24† 10 50 KH-01728-60

WhiteNontreated Round 360 µL No No 25 100

KH-01728-64 Flat 100 KH-01728-65 High binding Flat 360 µL No No 25 100 KH-01728-63 Cell culture Flat 360 µL Yes Yes 20 100 KH-01728-61

BlackNontreated Round 360 µL No No 25 100

KH-01728-66 Flat 100 KH-01728-67 High binding Flat 360 µL No No 25 100 KH-01728-62 Cell culture Flat 360 µL Yes Yes 20 100

Clear-bottom platesKH-01728-46 White Nontreated — — No No 25 — KH-01728-43 Cell culture — — Yes Yes 20 — KH-01728-44 Black Nontreated — — No No 25 — KH-01728-41 Cell culture — — Yes Yes 20 —

LidsKH-01730-01 Corner-notch, with condensation rings, clear PS, sterile 1 100 KH-01730-02 25 50

01728-01

01728-66

01728-64

01728-46 01728-44

*Plates have Certified Surface Chemistry. † Lids feature individual condensation rings that reduce well-to-well contamination.

‡Supplied with test certification


819



96-Well Non-Binding Surface Microplates Modified polymer surface (PS) creates a non-ionic, hydrophilic, polyethylene oxide-like surface that minimizes bio-interactionsl Significantly reduces protein and nucleic acid binding to polymers (<2 ng/cm2)l Maintains enzyme activity and inhibits adhesion of a number of cell linesl Enhances signal-to-noise ratio—ideal for homogeneous assays such as Scintillation

Proximity Assays (SPA)Half-area microplates are now available. They offer same superior performance at reduced volumes.

13021-48

01728-66

Catalognumber Application Plate color Number

of wellsQty/pk

Qty/cs

Price/cs

PlatesKH-13021-42 SPA White 96 25 100 KH-13021-44 White/clear bottom KH-13021-46

FluorescenceClear

96 25 100

KH-13021-48 Black KH-13021-50 Black/clear bottom

Half-area microplatesKH-13021-52 Fluorescence Black/clear bottom 96 25 100 KH-13021-54 Black KH-13021-56 SPA White/clear bottom 96 25 100 KH-13021-58 White

LidKH-01730-02 Corner-notch, clear PS, sterile 25 50


13021-50

13021-44

Catalognumber

Platecolor

Surfacetreatment

Wellshape

Wellvolume

Qty/pk

Qty/cs

Price/cs

PlatesKH-01728-66 Black Nontreated Flat 360 µL 25 100 KH-01728-67 High binding KH-01728-64 White Nontreated Flat 360 µL 25 100 KH-01728-65 High binding

LidsKH-01730-01 Corner-notch, with condensation rings, clear PS, sterile 1 100 KH-01730-02 25 50


13021-40

UV Plate Low background absorbance readingsl Unique plastic material is molded onto plate bottom, making it transparent to UV lightl UV transparent bottom helps determine nucleic acid and protein concentrations in

a 96-well formatl Compatible with Bio-Tek ELx Powerwave™ 200, Bio-Tek µQuant, Bio-Rad UltraMark™,

and Molecular Devices SPECTRAmax® 190 and 250 plate readers

Catalognumber

Numberof wells

Wellshape

Wellvolume

Qty/pk

Qty/cs

Price/cs

KH-13021-40 96 Flat 370 µL 25 50


96-Well Solid PlatesSelect high-binding surface for immmunoassay work; nontreated surface for solution-based assaysl Polystyrene plates are tested on a lot-to-lot basis for opacity and low background

fluorescence/luminescence—ensures minimal well-to-well crosstalkPolystyrene plates are available in two colors: white for optimizing imuinescence signal reflection, and black for minimizing background fluorescence and backlight scatter.

01728-64


820



96-Well Microplates, Immunology

Catalognumber

Wellshape

Wellvolume

Bindingproperty Lid Qty/

sleeveQty/cs

Price/cs

PlatesKH-01728-20 Round 0.33 mL Medium No 25 100 KH-01728-22 High KH-01728-26 Flat 0.36 mL Medium No 25 100 KH-01728-28* High LidsKH-01730-01 Corner-notch, with condensation rings,

clear PS, sterile1 100

KH-01730-02 25 50

Specifications & Ordering InformationClear, medical-grade polystyrene for research and in vitro diagnostic assaysl Formulated for uniform

binding, high optical clarity, and low background absorption

l Medium-binding plates offer a binding capacity of 250 ng of mouse IgG/cm2

l High-binding plates offer a binding capacity of 500 ng of mouse IgG/cm2

Stripwell™ PlatesPlates consist of twelve 1" x 8" strips and an “egg-crate”-style strip holderl Strips have one-way fit

into strip holder— prevents misorientation

l Flat-bottom strips break apart and can be reassembled into strip holder

l Manufactured from medical- grade polystyrene (PS)— ideal for uniform binding, high optical clarity, and low background absorption

High and Medium Binding Stripwell™ CertificationCertification standards High binding Low binding

Well-to-well Coefficient of Variation (CV) ≤3% ≤5%Average high and low wells from the mean ±8% ±15%

Background absorbance units from the mean ±0.005 ±0.005

13020-00

Clear PS Lids have a corner notch, with condensation rings.

AccessoriesStrip Holder is designed for use with any Costar® flat-bottom strips. Strip Ejector facilitates removal of strips from the holder.

Catalog number Description Sterile Qty/cs Price/csKH-01730-01 Individually packed Yes 100 KH-01730-02 Bulk packed Yes 50

Catalog number Description Sterile Qty/cs Price/csKH-13020-50 Strip holder No 20 KH-13020-52 Strip ejector No 5


Catalognumber Color Binding

propertyWell

volumeQty/cs

Price/cs

Stripwell platesKH-13020-00†

Clear High 360 µl 100 KH-13020-02† Medium

1 x 8 stripsKH-13020-12 Clear High 360 µl 800

Specifications & Ordering InformationCatalog number Well shape Well volume Sterile Lid sleeve Qty/cs Qty/cs Price/cs

Standard microplatesKH-01728-80 V-bottom 0.32 mL No No 25 100 KH-01728-81 Flat 0.36 mL No No 25 100 KH-01728-82 Round

Deep-well assay blocksKH-01729-02 Round 1.0 mL Yes No 5 25 KH-01729-04 No 100 KH-01729-03 V-bottom 2.0 mL Yes No 5 25 KH-01729-05 No 100

96-Well Polypropylene Storage System 96-well microplates and assay blocks, nontreatedl Constructed of clarified polypropylene for optimal clarity and solvent resistance—

ideal for compound library and combinatorial chemistry applicationsStandard microplates are built to industry standard footprint; compatible with automated systems. Deep-well assay blocks are ideal for higher-volume storage and assay applications. Use microplates and 1.0-mL assay blocks with the storage mat and applicator listed below. Plates and mats are certified RNase and DNase free.

01729-04

01728-80

KH-01729-22 Storage mat for 96-well polypropylene plates and 1.0-mL assay blocks. Pack of 25; case of 100KH-01729-24 Storage mat applicator

Storage mat applicator 01729-24

shown with storage mats

01729-22

Plate 01728-20 with lid 01730-01*These plates have Certified Surface Chemistry which guarantees protein binding performance.

†Plates have Certified Surface Chemistry


821



96-Well Microplates, NontreatedUsed in pharmaceutical, diagnostic, clinical, and research applications for high throughput screening, drug discovery, and combinatorial chemistryl Nontreated polypropylene plates are autoclavable, solvent resistant,

and withstand temperatures from –13 to 250°F (–25 to 121°C)l Plates have 86 x 128 mm footprint (except 01929-30)—

meets Society for Biomolecular Screening (SBS) guidelinesl Caps create a tight seal, but are easy to peel off without aerosols—

reducing the risk of well-to-well contamination. Caps are pierceable by hypodermic needles without compromising seal.


Catalognumber

Wellshape

Wellvolume Sterile Lid Qty/

sleeveQty/cs

Price/cs

PlatesKH-01929-30 Conical 0.3 mL No No 5 60 KH-01929-32 Round 0.5 mL No No 10 120 KH-01929-33 Yes KH-01929-34 Conical 0.45 mL No No 20 120 KH-01929-38 Yes

Polystyrene lids for plates 01929-32, -33, -34, and -38KH-01929-10 Nonsterile, with condensation rings, clear PS 5 100 KH-01929-11 Sterile, with condensation rings, clear PS 1 50

96-well caps for plates 01929-32 and -33KH-01929-56 Nonsterile, 96-well cap, natural PP 5 50 KH-01929-54 Sterile, 96-well cap, natural PP 1

01929-32 microplate with 01929-56 cap 01929-32

Plate Sealing TapeKH-01929-12 Plate sealing tape, sterile polyester. Minimizes moisture loss from samples during storage. Tape measures 80 x 134 mm; case of 200

96-Well Deep Well PlatesExcellent for high throughput screening, cell and tissue culture, sample storage, and morelWithstand temperatures as low as –121°C—ideal for cold-room applications and cryo-storagelAlphanumeric pattern for simple sample identificationlNotched corners facilitate orientation These stackable deep well plates are available with 0.5 mL, 1.1 mL (1.0 mL capped), and 2.2 mL (2.0 mL capped) well volumes. Manufactured from high-quality virgin polypropylene, these plates resist a wide variety of chemicals, including phenols, chloroform, and DMSO. They are compatible with most robotic samplers and automated liquid handling systems. Polypropylene plates are autoclavable at 250°F (121°C) for 20 minutes.

Catalog number Material Well shape Well volume Sterile Qty/pk Price/pkKH-07904-02

Polypropylene Round 0.5 mL

No

48 KH-07904-04 1.1 mL 24 KH-07904-06 Square 2.2 mL 24 KH-07904-08 Polystyrene Round 1.1 mL 32

96-Well Microtitration PlatesIdeal for cell and tissue culture, sample storage, and immunological assaysBoth sterile and nonsterile plates are available with rounded, V-shaped, or flat well bottoms to meet the needs of most applications. Sterile, treated plates with hydrophilic surfaces are also available. Nonsterile polypropylene microtitration plates are autoclavable and resistant to a wide variety of chemicals including phenols, chloroform, and DMSO. Wells are labeled in a standard alphanumeric pattern to simplify sample identification and are stackable for easy storage.

Catalog number Material Surface

treatment Well shape Well volume Sterile Packaging Qty/pk Price/pk

KH-07903-80Polystyrene No

Round 330 µLNo 20 bags of

5 plates 100

KH-07903-82 V-shaped 330 µL KH-07903-84 Flat 400 µL KH-07903-94

Polystyrene NoRound 330 µL

Yes 20 bags of 5 plates 100

KH-07903-96 V-shaped 330 µL KH-07903-98 Flat 400 µL KH-07903-88

Polystyrene YesRound 330 µL

Yes Individually wrapped

100 KH-07903-90 V-shaped 330 µL 60 KH-07903-92 Flat 400 µL 60

KH-07904-00 Polypropylene No Round 330 µL No 10 bags of 10 plates 100



KH-07903-86 Plate lids, polystyrene. 20 bags of 5 plates, pack of 100

07904-02

07903-80


822



Prevents evaporation and protects sample during processing, storage, and shipmentl Superior quality film and uniform, consistently applied adhesivel Two removable end tabs for easy and accurate positioning on microplatel Functional temperature range from –40 to 120°CSealPlate 50-µm polyester films minimize evaporation, prevent spillage and contamination between wells, and provide a secure seal, not just a cover. Secure sealing of all wells eliminates edge effects in sensitive ELISA assays. Films can also be used with tissue culture plates for short-term storage, incubation, and containment of biohazards. Films are non-pierceable. Each film measures 79.4 x 146.0 mm.

MiniStrips™ films (13024-04 and -06) are identical to the microplate films, but are designed for sealing only one or two 8-well rows at a time on either strip-well plates or standard plates whenever rows must be selectively protected or accessed. Also use as a secondary seal to cover films that have been pierced. Each film measures 19.0 x 103.2 mm with the two removable positioning tabs; 19.0 x 82.6 mm without tabs.

Specifications & Ordering InformationCatalog number Description Sterile Qty/pk Price/pk

KH-13024-00 SealPlate films No 100 KH-13024-02 Yes KH-13024-04 MiniStrips films No 200 KH-13024-06 Yes

13024-00

13024-04

SealPlate® Films for ELISA, EIA, and Microplates

EZ-Pierce® Films for MicroplatesPrinted alphanumeric template version simplifies well locationl Functional temperature range -40 to 90°C l Inert, chemical resistantThese 70-µm thick polyethylene with acrylic adhesive films are easily pierced—allowing direct sample recovery with single or multichannel pipettors and robotic probes. Films are available with or without printed 96-well alphanumeric template for easy well location. Each film has white marking area on each end tab for plate identification. Measure 82.6 x 142.9 mm without end tabs.


Catalog number Printed alphanumeric template Sterile Qty/pk Price/pkKH-13024-08 No No 100 KH-13024-10 Yes

13024-08

13024-10

Syringe PumpsIdeal for low-flow applications where precision is neededl Obtain accurate, pulse-free flow from 1 nL/min to

363.7 µL/hr with the 74902-series l Models accept syringes from 0.5 µL to 200 mL l Preset rate and volume control l Remote injector and rugged mounting arm on the 74902-series l Microprocessor motor control and precision gearing provide reliable pulseless flow l Wide dynamic range (6000:1) provides excellent flexibilityl Program all commands via the front-panel keypad you can even change settings while

the pump is running 74900-00

74902-00

For a complete selection of our syringe pumps, see our “Metering Pumps” section.

1603–1607


823



36490-22


Catalog number Description Price

KH-36490-20SuperSignal pico

chemiluminescent substrate

KH-36490-22SuperSignal ELISA

femto chemiluminescent substrate

Chemiluminescent HRP Substrates for ELISAExcellent sensitivity for use in luminometersThe Chemiluminescent substrates are designed for enzyme detection and quantification for researchers looking for greater sensitivity in their ELISAs or any other solution-based assay. These ELISAs can take place in either a test tube or a microplate. They are quantified by measuring relative light units (RLU) in a luminometer. Model 36490-20 was developed for researchers who need high sensitivity at an economical price. Model 36490-22 has an improved enhancer system that meets the needs of high-throughput screening and diagnostic customers.

SuperSignal® ELISA Pico Chemiluminescent Substratel Determine protein concentrations in under five minutes This substrate is optimized for lumino meter-based assays to generate an intense light signal and can be read at 425 nm. An intense signal is produced immediately at room temperature or at 37ºC. High signal-to-noise ratio minimize the background. Signal can be read in black or white opaque plates. Kits can be store at room temperature.

SuperSignal® ELISA Femto Chemiluminescent Substratel The most sensitive substrate for plate-based applications This kit utilizes an enhancer formulation for superior protein detection in ELISA applications with improved low-end linearity. Intense signal is generated immediately at both room temperature and 37°C. Signal can be read at 425 nm.

Kit also features improved low-end linearity which ensures easy detection of low quantities of proteins with high signal-to-noise ratios and low-end linearity of dose response curves. Stores up to six month at room temperature or a minimum of 12 months at 4°C with six-hour working solution stability.

What’s included: 50 mL fluminol/enhancer and 50 mL of stable peroxide solution.

Which substrate is right for you?Substrate Detection Limits Kinetics Working solution stability

at room temperature

ELISA Pico Chemiluminescent substrate Quantitative to the picogram level light

Immediate light generation 8-hour working solution stability, only 10% loss of activity after 24 hours30-minute stable activity after 24 hours

ELISA Femto Maximum sensitivity substrate Quantitative to the femtogram level

Immediate light generation 6-hour working solution stability5- to 30-minute stability depending on HRP concentration

SealPlate® Films with ColorTab™ Color-Coded End TabsColor dots and white labeling area on end tabs for plate ID labelingl Second tab can be separated for lab notebook insertionl End tabs can be left on plate even if center of film is removedl Functional temperature range is –40 to 120°C These nonpiercable films are made of 50-µm thick clear polyester. Use to minimize evaporation, prevent spillage and contamination between wells during ELISA EIA, and similar assays. End tabs with backing removed adhere to the ends of the plate and are perforated so they can be left in place to maintain plate identity even if the center portion of the film is removed. Films feature two colored dots and two white end tables per sheet. Each film sheet measures 79.4 x 146.0 mm.

Catalog number Cot color Sterile Qty/pk Price/pkKH-13024-22 Green

No 100

KH-13024-24 Lavender KH-13024-26 Orange KH-13024-28 Red KH-13024-30 Blue KH-13024-32 Assorted* No 50 KH-13024-34 Yes

13024-22


*Assorted packs contain ten films of each of the five colors listed above.

ColeParmer.com/lifescienceFor new products, tech info, applications, and promotions.


824


LI Life Science

North2South® Direct Kit for Northerns and SouthernsA sensitive two-hour chemiluminescent blotting kit l Two-hour start-to-finish protocol, faster than biotin or DIGl As sensitive as radioactive methods and more sensitive than

other non-radioactive direct-label systems

This system is designed for the chemiluminescent detection of biotinylated probe on Northern and Southern blot or colony plaque lift applications. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After blocking the membrane, the biotin label is detected with streptavidin-HRP (horseradish peroxidase) and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray file (1 to 15 minutes) or CCD camera. The kit provides sensitivity equal to or greater than 32P and is amenable to many formats.

What’s included: sufficient detection reagents for ten 10 x 10 cm membranes (membranes not included).

02905-70


North2South® Chemiluminescent Hybridization and Detection Kit for Northerns and SouthernsNon-isotopic assay delivers sensitivity, consistency, and flexibilitylSensitivity equivalent to 32P lNon-radioactive alternativelExceptional speed—a 60-minute

post-hybridization time combined with immediate signal generation and shorter film exposures

02905-60

North2South Direct Kit vs. 32P Radiolabel

System Hybridization ExposureNorth2South direct kit 1 hour 1 minute

32P Radiolabel 24 hours 24 hour exposure with screens

32P RadiolabelNorth2South direct kit

Southern blot of yeast genomic DNA. Hind III digest (0.8 to 0.1 µg/lane) hybridized with 5 ng/mL HRP-labeled (PCR) probe vs.

a 3 ng/mL [32P]-dCTP labeled PCR probe.

The North2South direct HRP (horseradish peroxidase) kit is based on the patented chemiluminescent substrate technology that provides the ultimate in nonradioactive sensitivity. Features are enhanced with a one-step, 15-minute, direct-labeling procedure that is as simple as radioactive labeling. Labeled probes last for a year instead of weeks. The ready-to-use North2South Direct HRP Labeling and Detection Kit includes all required to label your probe with HRP, all necessary hybridization and wash buffers, and ultrasensitive (femtogram level detection) chemiluminescent substrate. All you do is provide the nucleic acid (DNA, RNA or oligonucleotide >50 bp) and some water.

What’s included: sufficient labeling reagents supplied for 10x10 ng labeling reactions and sufficient detection reagents supplied for 1,000 cm2 of membrane.


Catalog number Description PriceKH-02905-60 North2South direct labeling and detection kit

DNase-, RNase-, and Pyrogen-Free TipsClear tips are certified sterile, DNase-, RNase-, and pyrogen-free to prevent sample contamination. Tips fit a variety of pipettors to ensure laboratory standardization. Conveniently packed in sterile racks. For full specifications, go to pages 1134–1163.

Cat. no. Volume Pack description Price/pkEK-25713-40 0.1 to 10 µL Ten racks of 96 tipsEK-25713-42 0.5 to 10 µL Ten racks of 100 tipsEK-25713-44 1 to 200 µL Ten racks of 96 tipsEK-25713-46 1 to 200 µL, graduated Ten racks of 96 tipsEK-25713-48 101 to 1000 µL Ten racks of 100 tips

Catalog number Description Price

KH-02905-70 North2South chemiluminescenthybridization and detection kit

Molecular Biology


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LILife Science

Catalog number Description Amount sufficient for PriceKH-02905-80 LightShift™ Chemiluminescent EMSA/gel shift kit 100 binding reactions KH-02905-82 Biotin 3´ end DNA labeling kit 20 labeling reactions KH-02905-84 NE-PER nuclear and cytoplasmic protein extraction reagents 50 cell pellet extractions


LightShift® Chemiluminescent EMSA/Gel Shift Kit Fast and sensitive analysis of protein: DNA binding region and affinity l Biotin end-labeled DNA probes eliminate radioactive hazards and waste disposal l Sub-femtomole sensitivity and low background noise l One-day assay from labeling DNA to gel-shift data analysis

The LightShift EMSA/Gel Shift Kit This kit includes reagents for setting up and customizing protein:DNA binding reactions, a control DNA target and corresponding control protein extract, buffers, conjugate and luminol reagents for the detection phase of the assay. Custom target biotin labeled DNA can be prepared using the Biotin 3´ End DNA labeling kit (02905-82) or ordered directly from a custom oligo supplier.

What’s included: sufficient components for 100 binding reactions and detection. Reagents for 800 cm2 of membrane (membranes not included).

Biotin 3´ End DNA Labeling Kit A complete kit for labeling DNA on the 3´ end with biotin. This kit uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3´-OH end of DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3´ overhangs and blunt duplexes, albeit with a lower efficiency.

Boitin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.

NE-PER Nuclear and Cytoplasmic Extraction Reagents Facilitate the separation of nuclear extracts and cytoplasmic fractions from the same set of cells—or tissue in less than two hours. This kit obtains nuclear and cytoplasmic extracts from cells—even the same set of transfected cells—or tissue in less than two hours, saving time and money. It maintains nuclear and cytoplasmic cell compartments by lysing the cell membrane, recovering the nucleus intact, and then extracting nuclear proteins separately. Use nuclear extracts and cytoplasmic protein fractions directly in a variety of applications including reporter gene assays, enzyme activity assays, Western Blotting and gel shift assays.

02905-80

Bovine Serum Albumin StandardThe most reliable protein standard for total protein assayslSealed ampules ensure freshness and reliability l2 mg/mL concentration is calibrated to an NIST standard for consistency

What’s included: bovine albumin fractin V in 0.9% NaCl solution containing sodium azide.

36490-10


Catalog number Description Size PriceKH-36490-10 Albumin standard ampules 10 x 1 mL

Comparison of the LightShift® EMSA Kit to a digoxigenin-based kit and a radioactive method

ColeParmer.com/lifescienceFor new products, application notes and promotions

Molecular Biology


826


LI Life Science



Catalog number Power PriceEK-83057-30 115 VAC, 60 Hz EK-83057-35 230 VAC, 50 Hz

This room temperature stable version of the BCA protein assay is the best method for measuring low concentrations of protein. The kit provides consistent, reliable results with less protein-to- protein variation than dye binding methods. Use the assay to measure protein concentrations as low as 0.5 µg/mL in either a microplate or a cuvette.

What’s included: reagents and BSA standard, sufficient to perform up to 480 standard or 3200 microplate assays.


Catalog number Description PriceKH-36490-00 BCA protein assay kit

36490-00

Ordering Information36490-04

BCA Protein Assay KitTotal protein determination in the presence of detergentsl Four times faster and easier

than the Lowry method l Colorimetric method; read at 562 nm l Linear working range for BSA

from 20 to 2000 µg/mLThe room temperature stable BCA protein assay is the most popular method for determining total protein concentration. The kit provides consistent, reliable results with less protein-to-protein variation than dye binding methods. Use the assay to measure protein concentrations from 5 µg/mL up to 2000 µg/mL in either a microplate or a cuvette.

What’s included: reagents and BSA standard, sufficient to perform up to 500 standard or 5000 microplate assays.

Coomassie Plus™ Protein Assay KitDetermine protein concentrations in less than 5 minutes lColorimetric method; read at 595 nmlLinear working range for BSA from 1 to 1500 µg/mLlCompatible with reducing agentsThe room temperature stable Coomassie Plus® protein assay is the easiest method for determining total protein concentration. The kit provides considerable improvement to the traditional Bradford method and can be used in either a microplate or a cuvette.

What’s included: reagent and BSA standard, sufficient to perform up to 630 standard or 3800 microplate assays.

Which protein assay is right for you?Protein assay Working range Accuracy Assay time Compatibility

BCA Kit 20 to 2000 µg/mL High 30 minutes Brij-35, CHAPS, deoxycholate, NP-40, SDS, Micro BCA Kit 0.5 to 20 µg/mL High 60 minutes Triton x-100, Tween-20, and other detergents

Coomassie Plus Kit 1 to 1500 µg/mL Medium 10 minutes Ascorbic acid, cysteine, DTT, glucose, 2-mercaptoethanol, potassium thiocyanate, and other reducing agents

36490-02

Scanning UV/Visible Spectrophotometerl Advanced spectral

analysis—DNA, protein, kinetics, and more!

l Sophisticated methods with graphic display

l Programmed GLP verification protocols

Specify method parametric setup using simple on-board keypad controls or from computer. Sample compartment holds up to 8 cuvettes with pathlengths up to 100 mm.

Store up to 30 user methods in nonvolatile memory. Programmed methods include DNA/protein ratios (260/280 nm or 260/230 nm). Specify up to 10 wavelengths for multiple sample analysis or use the GLP protocol to verify wavelength and transmittance. Scan speed is adjustable from 75 to 1000 nm/min. Transfer data directly to printer using parallel port or interface with computer using RS-232 port. See page 1586 for complete information and specifications on this meter.

What’s included: 4-cell manual 10 mm cell changer, four glass and two silica 10 mm cuvettes, power cord, and dust cover.

83057-30

Bandwidth: 4 nmRange: 190 to 1000 nmAccuracy: ±1 nmResolution: 0.1 nm

Repeatability: ±0.05 nmDrift: <0.002 A/hr after warmupStray light: <0.15% T at 220 and 340 nmSource lamp: Tungsten halogen/deuterium

Catalog number Description PriceKH-36490-02 Micro BCA protein assay kit

Catalog number Description PriceKH-36490-04 Coomassie plus protein assay kit

Micro BCA™ Protein Assay KitMost sensitive BCA formulation for measuring dilute proteinlColorimetric method;

read at 562 nmlLinear working range for

BSA from 0.5 to 20 µg/mLlCompatible with

most detergents

Molecular Biology


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LILife Science

Immobilized Streptavidin, NeutrAvidin™, and Monomeric Avidin Gelsl Use for simple isolation of biotin-labeled proteins, nucleic acids, and other biomoleculesThese immobilized proteins are covalently attached to agarose supports via reductive amination—resulting in a stable, leach-resistant linkage that is uncharged and does not impart any ion-exchange properties to the support. Streptavidin has a high affinity for biotin, is carbohydrate-free, and has relatively little nonspecific binding. NeutrAvidin has an equally high affinity for biotin and it displays even greater specificity for biotin, yielding the lowest nonspecific binding among avidin proteins. Monomeric avidin (supplied with a disposable column trial kit) is also specific for biotin but binds with a much lower affinity, allowing release of biotin-labeled molecules from the support under gentle conditions.

Properties of biotin-binding proteinsStreptavidin NeutrAvidin Monomeric

avidinMolecular weight 53,000 60,000 67,000Number of biotin binding sites 4 4 1Isoelectric point (pI) 6.8 to 7.5 6.3 10Specificity High Highest MediumAffinity for biotin (Kd) 10–15 M 10–15 M 10–8 M

Specifications & Ordering InfoSupport media: cross-linked 6% beaded agarose

Cat. no. Description Capacity Qty/pk Price/pkKH-36490-30 Streptavidin gel 1 to 3 mg biotinylated BSA/mL of gel

5 mL

KH-36490-32 NeutrAvidin gel ≥20 µg biotin/mL of gel KH-36490-34 Monomeric avidin gel ≥1.2 µg biotinylated BSA/mL of gel

36490-3436490-30 36490-32

Properties of antibody-binding proteins

ImmunPure® Immobilized Protein A, G, A/G, and LIdeal for antibody research and small-scale production using affinity purification methods l Provide the widest range of purification optionsHighly purified forms of proteins are covalently attached to agarose supports via reductive amination; resulting in a stable, leach-resistant linkage that is uncharged and does not impart any ion-exchange properties to the support. Proteins A and G are bacterial cell wall components that bind primarily to the Fc region of antibodies and are by far the most popular choice for affinity purification of IgG. Protein A/G is an engineered protein that combines the IgG binding domains of both Proteins A and G. Protein L is another bacterial protein used for antibody purification; it binds to several classes of immunoglobulin (e.g. IgG, IgM, IgA) if they have particular kappa light chains.

36490-40 36490-50

Native Protein A Recombinant Protein G

Recombinant Protein A/G

Recombinant Protein L

Source Staphylococcus aureus Streptococci Bacillus Peptostreptococci

Molecular weight 42,000 22,000 50,449 35,800Number of binding sites for IgG 4 2 4 4

Albumin- binding site No No No No

Optimal binding pH 8.2 5 5 to 8.2 7.5

Binding location on IgG Fc Fc Fc VLK

Catalognumber Description Capacity

(mg human IgG/mL of gel)Qty/pk

Price/pk

KH-36490-40 Protein A 12 to 19 5 mL KH-36490-42 Protein A Plus ≥35 KH-36490-44 Protein G 11 to 15 2 mL KH-36490-46 Protein G Plus ≥20 KH-36490-48 Protein A/G ≥7 3 mL KH-36490-50 Protein L 4 to 5 2 mL KH-36490-52 Protein L Plus 8 to 10

Specifications & Ordering InfoSupport media: cross-linked 6% beaded agarose

36490-44

ColeParmer.com/MolecularBiologyFor the latest products on the web.

Reach™ Extended-Length Pipette TipsThe longer tip reaches to the bottom of sample tubes without letting the pipettor barrel touch the sides of the tube. A patented self-sealing barrier prevents cross-contamination from liquids and aerosols. Go to pages 1134–1163 for more information or call our Application Specialists.

Molecular Biology


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LI Life Science

Catalog number Description Qty/pk PriceKH-29951-20 Montage single-sample PCR cleanup kit 50/pk

29951-20



Catalog number Description Qty/pk PriceKH-29951-10 Micropure-EZ enzyme remover with vial 24

KH-29951-12Micropure-EZ enzyme remover

without vial, for use with Microcon filter units

24

Single-Sample PCR Cleanup Kit Quick protocol—one 15-minute spin followed by sample reconstitution

Micropure-EZ Enzyme RemoverOne-step, 60-second DNA purificationl Simple protocol, minimal

hands-on time l High DNA recovery (>85%) l Avoids use of phenolThis product is designed for removal of restriction and other enzymes from double-stranded DNA prior to electrophoresis. The devices’ membrane has a high affinity for protein but not for dsDNA. In a single 60-second spin, enzymes from the reaction mix are selectively adsorbed and DNA is recovered in a vial—undiluted and enzyme-free. For concentrating and desalting the sample at the same time, add a Microcon filter unit (order separately below).

GenSpin™ Kits for Genomic and Plant DNA PurificationExtract high-quality, PCR-ready DNA in less than 25 minutes!l FTA technology lyses cells and protects the DNA from

degradation—enables long-term storage at room temperaturel Single tube design ensures safe transport of samplesGenSpin Genomic DNA Kit purifies genomic DNA from whole blood or cultured cells. Kit uses a single microcentrifuge tube and small sample volume (5 to 50 µL) to produce high-quality DNA for PCR amplification.

GenSpin Plant DNA Kit eliminates problems associated with polysaccharides, secondary metabolites, and liquid nitrogen extraction. Use as a tool for field collection, genotyping of cultivars, and quick ID of genetically modified plants.

What’s included: 50 DNA isolation filter baskets in 2 mL microcentrifuge tubes, 50 x 2 mL microcentrifuge tubes, 110 mL GenSpin buffer, and 25 mL nuclease free water.

36204-00

Ordering InformationCatalog number Power Price

KH-36204-00 GenSpin genomic DNA purification kit KH-36204-02 GenSpin plant DNA purification kit


Gel Extraction Kit Fully functional DNA extracted from agarose gels in just a 10-minute spinRecover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, and drives the agarose through a small orifice in the gel nebulizer. The resultant gel slurry is sprayed into the sample filter cup.

Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

29951-30

Catalog number NMWL* Color Qty/pk Price/pkKH-29950-50 10,000 Green

24

KH-29950-52 30,000 Clear KH-29950-54 100,000 Blue

Microcon Filter Units

l Recommended for PCR products >300 bp

l No solvents or salts required

Use this kit to remove primers and unincorporated dNTPs prior to sequencing or cloning. Filter unit offers excellent capacity and high purity. Use final purified products for sequencing and cloning applications. Maximum initial sample volume is 500 µL (0.5 mL).

29951-10

Catalog number Description Qty/pk PriceKH-29951-30 Gel extraction kit 50/pk

For a wide selection of pipettes and tips, see our “Pipettes and Pipettors” section on pages 1134–1163.

1134–1163

*Nominal molecular weight limit

Molecular Biology


829


LILife Science

SpinColumns™ Centrifuge FiltersUse for easy, small sample separation with a standard centrifugel Single-use, disposable chromatographic materials in centrifuge tube formatsl Rapid preparation time and high sample recovery l Quickly recover samples based on the physical properties of the sample

A-B Ultra-Micro and Micro SpinColumns FiltersSelect Ultra-micro Spincolumns for 0- to 25-µL sample volumes; select micro SpinColumns for 25- to 75-µL sample volumes. Each column includes two 2.0-mL centrifuge tubes and top cap.

C Macro SpinColumns FiltersUse macro SpinColumns for 75- to 150-µL sample sizes. Each column includes two 2.0-mL centrifuge tubes and top cap and bottom cap.

D 96-Well SpinColumns FiltersThese SpinColumns are ideal for high-throughput sample preparations and more time-efficient sample recovery. Use 96-Well SpinColumns for 25- to 100-µL sample sizes. Includes two 96-well collection plates (1.5 mL per well).

96-well SpinColumn 02897-80

SpinColumns centrifuge filters offer a wide selection of prepackaged chromatographic materials in disposable centrifuge tube formats. Applications include sample concentration; protein, peptide, or DNA purification; buffer exchange; and salt and radiolabel removal.

Each column contains one of a wide range of column materials held in place by a frit. Simply place the SpinColumn filter in a centrifuge tube and centrifuge briefly to separate your sample. The column material binds and purifies the sample according to size and shape, chemical composition, charge, or other physicochemical properties. Results obtained are highly reproducible, and there is minimal sample loss.

Immobilized Metal Affinity Chromatography (IMAC) packing material is used for purification of histidine-tagged proteins. Other types of column materials are available—call our Application Specialists for more information.

Specifications & Ordering InformationSample volume

Ultra-Micro SpinColumn: 10 to 25 µL Micro SpinColumn: 25 to 75 µL Macro and 96-well SpinColumn: 75 to 150 µL

Tip volume Macro SpinColumn: 1000 µL All other columns: 300 µL

Body material: polypropylene

Frit material: polyethylene

02897-20 02897-24

Macro SpinColumn

02897-40

02897-0402897-00

BA

C

D

Type Tip packingmaterial

A Ultra-Micro SpinColumn B Micro SpinColumn C Macro SpinColumn D 96-well SpinColumnCat. no. Price/pk of 100 Cat. no. Price/pk of 100 Cat. no. Price/pk of 100 Cat. no. Price/ea

Gel filtration G25 = 5KD KH-02897-00 KH-02897-20 KH-02897-40 KH-02897-80 G50 = 30KD KH-02897-02 KH-02897-22 KH-02897-42 KH-02897-82

Hydrophobic (non polar) C18 (Vydac) KH-02897-04 KH-02897-24 KH-02897-44 KH-02897-84 Hydrophilic (polar) NH2 KH-02897-06 KH-02897-26 KH-02897-46 KH-02897-86

Ion exchange

Strong anion(quaternary amine) KH-02897-08 KH-02897-28 KH-02897-48 KH-02897-88

Strong cation(sulphopropyl) KH-02897-10 KH-02897-30 KH-02897-50 KH-02897-90

Miscellaneous IMAC KH-02897-12 KH-02897-32 KH-02897-52 KH-02897-92

Molecular Biology


830


LI Life Science

B Vivaspin 6 for 2 to 6 mL Samples

Vivaspin 6 features twin vertical membranes for unparalleled filtration speeds and 100x plus concentrations. Remaining volume is easy to read from the printed scale on the side of the concentrator and the modified dead stop pocket further simplifies direct pipette recovery of the final concentrate.

D Vivaspin 20 for 5 to 20 mL Samples

Vivaspin 20 handles up to 20 mL in swing bucket centrifuges and 14 mL in fixed-angle rotors accepting 50 mL centrifuge tubes.

A Vivaspin 500 for 100 to 600 µL Samples

The 500 µL centrifugal filter units can be used in either swing out or fixed-angle rotors accepting 2.2 mL centrifuge tubes. The patented vertical membrane design and thin channel filtration chamber minimize membrane fouling and provide high speed concentrations, even with particle laden solutions.

*Choose a pore size at least 50% smaller than the size of the molecule to be retained.

KH-36224-90 Diafiltration cups for desalting. Pack of 12KH-36224-91 Pressure head KH-36224-92 Air pressure controller KH-36224-93 Charge valve for pressure head

C Vivaspin 15 R for 2 to 15 mL Samples

Vivaspin 15 R has a modified regenerated cellulose membrane specially designed for “sticky” samples. This membrane has ultimate recovery at low adsorption (95 to 98%) and very small hold up volume (<20 µL). Ideal for desalting and concentration of Ig fractions.

C36228-32

D 36224-80

Vivaspin™ Centrifugal Concentrators with PES MWCO MembranesBroad range of process alternatives for the filtration and concentration of biological samplesl Disposable ultrafiltration devices concentrate and purify biological samples to concentrations

over 100x in a single spin!l Concentrators feature twin vertical membranes—increased surface area provides faster filtration l Polyethersulfone membrane is excellent for most solutions when recovery is most important

A36228-00

B 36228-16

Catalog number MWCO Qty/pk Price/pkKH-36228-16 5,000 Dalton

25

KH-36228-18 10,000 Dalton KH-36228-20 30,000 Dalton KH-36228-22 50,000 Dalton KH-36228-24 100,000 Dalton

25

KH-36228-26 300,000 Dalton KH-36228-28 1,000,000 Dalton KH-36228-30 0.2 µm


25

KH-36228-34 10,000 Dalton KH-36228-36 30,000 Dalton

Catalog number MWCO* Qty/pk Price/pk Catalog number Qty/pk Price/pkKH-36224-70 5,000 Dalton

12 KH-36224-71

48

KH-36224-72 10,000 Dalton KH-36224-73 KH-36224-74 30,000 Dalton KH-36224-75 KH-36224-76 50,000 Dalton

12 KH-36224-77

48

KH-36224-78 100,000 Dalton KH-36224-79 KH-36224-80 0.2 µm KH-36224-81


25

KH-36228-02 10,000 Dalton KH-36228-04 30,000 Dalton KH-36228-06 50,000 Dalton KH-36228-08 100,000 Dalton

25

KH-36228-10 300,000 Dalton KH-36228-12 1,000,000 Dalton KH-36228-14 0.2 µm

Molecular Biology


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29950-50

Specifications & Ordering InformationMembrane materials: Ultracel-YM regenerated cellulose Membrane support base: acetalSample reservoir: polycarbonate Sample vial: polypropylene

Microcon® Centrifugal Filter UnitsHighest recovery of sample with solute recoveries typically > 95%Ideal for dilute (ng/mL to µg/mL range) protein solutions. Use in standard fixed-angle rotors that accept 1.5 mL centrifugal tubes. Applications include concentrating and purifying proteins, antibodies, and nucleic acids (alternative to EtOH precipitation); desalting and buffer exchange; and removal of primers, linkers, and unincorporated label. Low-binding ultracel-YM regenerated cellulose membranes are available with different molecular weight cut-offs—call our Application Specialist for more information.

Maximum initial sample volume is 500 µL with typical final concentrate volume of 5 to 15 µL. Filter units withstand up to 14,000 x g RCF; 500 x g RCF for 29950-54 on DNA/RNA applications.

*Nominal Molecular Weight Limit

Catalog number NMWL* Color Qty/pk Price/pkKH-29950-50 10,000 Green

24

KH-29950-52 30,000 Clear KH-29950-54 100,000 Blue

Nobuto Blood Filter StripsIdeal for blood collection applicationsCollect blood on the narrow end of the strip and place on the drying rack for easy and efficient sample handling. Each strip can hold approximately 0.1 mL of blood or 0.04 mL of serum. Strips are produced with high-purity cellulose paper with a fine, textile like structure—ideal for uniform sampling. Typical blood collection applications are: toxoplasmosis (HA), hanta virus, new castles disease (HI), canine distemper (VN), measles (Rubeola), Japanese encephalitis (HI, VN), mycoplasmosis (AG), and hog cholera (VN). Dried samples can be analyzed, stored, or mailed without refrigeration.

KH-06644-42 Filter strip drying rack

Catalog number Description Qty/pk Price/pkKH-06644-40 Nobuto blood filter strips 100


Nobuto blood filter strip 06644-40 with drying rack 06644-42

DNA Sponge™ Sampling SwabsSterile and individually wrappedSampling swabs are made of urethane foam with a polystyrene (PP) shaft, and are manufactured under the strictest quality controls in ISO 9002 registered facility. Sterile and for single use only. Ideal for wildlife forensic biology, DNA collection, Buccal cell collection, rapid diagnostic, paternity, and evidence collection. The urethane foam swab has been proven to be more effective than cotton swabs for sampling for DNA in blood.

Catalog number Description Package size PriceKH-14001-62 Regular foam tip on 6" PP shaft 100/bx KH-14001-60 1000/cs KH-14001-66 Round foam tip on 5" PP shaft 50/bx KH-14001-64 500/cs


14001-64

14001-60

ColeParmer.com/lifescienceFor new products, application notes and promotions

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Collect, isolate, archive, and transport nucleic acids— all at room temperature l Ideal for collecting and transporting samples

from remote locationslCollected sample stays stable at room

temperature for up to 14 years—freeing precious freezer space

lNo precipitation steps are needed; DNA and nucleic acid are ready for immediate downstream analysis

FTA® Cards come in four, two and one sample area size of 125 µL whole blood or 25 µL plant homogenate each. Classic cards are ideal for same specimen or multiple animal or plant samples on one card. Mini cards are ideal for protocols that require different locations for testing and archiving samples. Process each sample from classic and mini cards independently. Micro cards are designed when only one sample is needed.

CloneSaver™ Cards have a 96 well format designed for collecting, storing and purifying plasmid and BAC DNA from bacterial clones. The sample area accepts 96 x 5 µL of bacterial culture, resuspended colony or glycerol stock. Plasmid DNA on CloneSaver cards is stable at room temperature for at least three and half years.

Blood Stain Cards have four sample areas for 500 µL. Use to prepare blood samples for DNA analysis. Simply apply a blood, bone marrow or buffy coat sample to the card, DNA is captured and stabilized immediately, no toxic chemicals are required. DNA remains immobilized on the matrix and ready for PCR. No further purification is required, just punch a disk and wash with FTA purification reagent (36203-21). Cards are ideal for diagnostic and clinical applications, genetic identification, molecular screening studies, animal breeding studies and HLA typing.

FTA cards are available in a variety of sizes and formats

AccessoriesFTA Purification Reagent removes heme, PCR inhibitors, and other potential contaminants to ensure superior quality DNA for downstream analysis.

KH-36203-21 FTA purification reagent, 500 mL KH-36203-22 Sterile applicators, foam tipped. Pack of 50

Micro Punches with Cutting Mat are used for the precise sample disc removal from FTA cards. The 1.2-mm punches are recommended for use with whole blood and samples with high DNA content. The 2.0-mm punches are recommended for use with buccal cells, plasmids, and samples with lower DNA content.

KH-36203-23 Micro punch with cutting mat, 1.2 mm KH-36203-24 Micro punch with cutting mat, 2.0 mm

Disposable Multi-Barrier Pouches are used for transporting or storing FTA cards. They protect cards from environmental contamination. Tamper-evident seal maintains sample security for forensics samples. A resealable pouch is also available when multiple access to FTA cards is needed.

KH-36203-25 Disposable multi-barrier pouch, large. Use with FTA classic card 36203-00. Pack of 500KH-36203-26 Disposable multi-barrier pouch, small. Use with FTA mini and micro cards 36203-05 and -10. Pack of 500KH-36203-27 Resealable multi-barrier pouch, for CloneSaver card 36203-15. Pack of 50KH-36203-28 Desiccant packets with color indicator, 1 g. Use with all multi-barrier pouches. Pack of 1000

Catalognumber Card type Sample

volumeTotal sample

areaCards/

pkPrice/

pkKH-36203-00 FTA Classic 4 x 125 µL 500 µL 100 KH-36203-05 FTA Mini 2 x 125 µL 250 µL 100 KH-36203-10 FTA Micro 1 x 125 µL 125 µL 100 KH-36203-15 CloneSaver, 96 well 96 x 5 µL 480 µL 5 KH-36203-20 Blood Stain 4 x 125 µL 500 µL 100


Whatman® FTA® and CloneSaver™ Cards

CloneSaver™ Card 36203-15


Catalog number

MWCO(Dalton)

Volume(mL) Color Qty/pk Price/pk

EK-02905-01

3500

0.1 to 0.5

Pink

10 EK-02905-03 0.5 to 3 10 EK-02905-05 3 to 12 8 EK-02905-07 12 to 30 6 EK-02905-30

70000.1 to 0.5

Green10

EK-02905-32 0.5 to 3 10 EK-02905-34 3 to 12 8 EK-02905-40

10,000

0.1 to 0.5

Orange

10 EK-02905-42 0.5 to 3 10 EK-02905-44 3 to 12 8 EK-02905-46 12 to 30 6

Slide-A-Lyzer® Dialysis CassettesRequire just half the time of dialysis tubing for sample size from 100 µl to 30 mLl No more knots or clips that can lead to leaking sample lossl Color-coded translucent frame permits smooth withdrawal

of every drop of samplel High surface area/sample volume ratio will dialyze twice as

fast as dialysis via conventional tubing

Slide-A-Lyzer dialysis cassettes are ideal for low molecular weight contaminant removal, buffer exchange, desalting, and concentration. The cassette membrane is composed of low-binding regenerated cellulose and features a hermetically sealed sample chamber to maintain the highest possible sample retention. Sample introduction and removal are easily accomplished by penetrating the gasket with a hypodermic needle attached to a syringe. The gasket imparts resealability to the needle puncture, ensuring that no sample is lost from the cassette during dialysis.

02905-0102905-4402905-32

For a complete selection of dialysis products on pages 369–373.

369–373

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01840-00

01840-04 shown with 01840-14

01840-40 01840-46

Nunc offers a full range of microarray slides, plates, and accessories to provide researchers with consistent performance and results in microarray analysis. All items are made of high-quality polymer or glass and are available with a variety of different surface treatments that are optimized for nucleic acid, protein, cell and tissue applications.

Black Polymer Microarray SlidesThese polymer slides can be used with standard microarray printing equipment.

MaxiSorp™ Slides feature a charged surface optimized for proteins and is ideal where very low concentractions are to be quantified. They also feature low background fluorescence and improved signal-to-noise ratio versus glass slides. NucleoLink™ Slides feature a unique surface designed for nucleic acid array. Applications include immobilization of PCR products and cDNAs by baking; it is also optimal for SNP applications. Surface with immobilized nucleic acids can be stored for a prolonged period without affecting the subsequent hybridization.

Glass Microarray SlidesThese glass slides are available with surface coatings of aminopropylsilane, aldehyde, or epoxy. Epoxy-coated slide features an etched surface for consistent, uniform spot size. Low fluorescent background provides high signal-to-noise ratio. Slides are standard 25 x 75 mm size.

mSeries LifterSlip™ Glass SlidesThese rigid, 1-mm thick glass slides feature superior surface quality with consistent flatness, ensuring consistent fluid volumes and uniform dispersal of hybridization solutions. Volume capacities range from 20 to 55 µL. Applications include in situ hybridization and immunohistochemistry.

AccessoriesPolypropylene mBox 01840-14 is used for processing, transporting, and storing up to 20 slides. Box is sized so that it fits standard microwell carriers, allowing it to be centrifuged to remove excess liquid from the slides. Order the removable handle 01840-12 to easily immerse and retrieve slide box.

KH-01840-14 mBox with lid, polypropylene. Pack of 10KH-01840-12 Removable metal handle for mBox. Pack of 10

MicroArray Slides, Plates, and AccessoriesBarcoding available upon request for slide and data management

ArrayCote™ 16-Well Slides and 96-Well PlatesArrayCote products meet the growing demand for multi-welled arraying and can be scanned using traditional slide or plate instruments. Surface coatings are optimized for different applications; the type of coating is clearly labeled on each item to avoid mix-ups. Well dimensions are the same for the slides and plates, so scale up is easy.

The high-quality microarray slides feature a detachable, lidded polystyrene 16-well upper structure, glass slide base, and color-coded frosted writing area. Slides are standard 25 x 75 mm size and interface with existing readers. The 96-well plates have a lidded, black polystyrene upper structure and microarray-quality glass bottom.

Catalog number Description Method of attachment Applications Qty/pk Price/pk

KH-01840-00 MaxiSorp microarrayblack polymer slides

Nonspecific adsorptionto hydrophilic surface

Proteins, antibodies 25

KH-01840-02 Nucleolink microarrayblack polymer slides

Covalent attachment of nucleic acids, using carbodiimide (EDC) chemistry, baking for cDNAs and PCR products

Nucleic acids, cDNAs,

PCR products25

Catalog number

Surface coating

Etchedsurface Method attachment Applications Qty/

pkPrice/

pk

KH-01840-04 Aminopropylsilane No Nonspecific,electrostatic adsorption

cDNA, PCR products,proteins 20

KH-01840-10 Aldehyde No Covalent, amine-modified DNA

Nucleic acids, SNPs, proteins 20

KH-01840-06 Epoxy Yes Covalent, amine-modified DNA Nucleic acids, proteins 20

Catalognumber

Surface coating

Writing areacolor

Method ofattachment Applications Qty/

pkPrice/

pkArrayCote 16-well slides

KH-01840-40 Aminopropylsilane (APS) Blue Nonspecific,

electrostatic adsorptioncDNA, PCR products,

proteins 16

KH-01840-42 Aldehyde (ALD) White Covalent, amine-modified DNA


KH-01840-44 Epoxy (EPO) Green Covalent, amine-modified DNA

Nucleic acids, SNPs,proteins 16

ArrayCote 96-well plates

KH-01840-46 Aminopropylsilane (APS) — Nonspecific,

electrostatic adsorptioncDNA, PCR products,

proteins 20

KH-01840-48 Aldehyde (ALD) — Covalent, amine-modified DNA


KH-01840-50 Epoxy (EPO) — Covalent, amine-modified DNA


01840-20 01840-14 with 01840-12

Catalog number Volume (µL) Dimensions (mm) Qty/pk Price/pkKH-01840-20 20 22 x 25 x 1 KH-01840-22 29 22 x 40 x 1 150 KH-01840-28 30 25 x 40 x 1 KH-01840-24 36 22 x 50 x 1 KH-01840-30 45 22 x 60 x 1 150 KH-01840-26 55 25 x 60 x 1

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Thermo currently offers one of the most extensive ranges of authorized PCR machines. From the low through-put PCR Sprint to the computer-controlled MBS Satellite system, various range of products are designed to provide value for money features that meet the demands of modern researchers.

A PCR Sprint Thermal Cyclerl Personal thermal cycler for users with

low throughput requirementslSmall footprint for maximum use of

available space l 0.2- or 0.5-mL block formats with 20 or

24 well capacity for flexible choice of sample format

The PCR Sprint thermal cycler is ideal for users who want to perform small amounts of PCRs on a regular basis. Fast and efficient, the PCR Sprint has two sample formats: the 0.2-mL block option has space for twenty-four tubes and the 0.5-mL block option has space for twenty tubes. The block can be interchanged if necessary by using the thumbscrews evident on the block surround.

This cycler employs robust Peltier technology with heating rates up to 3°C/sec with excellent uniformity and accuracy. Supplied with a heated lid as standard for oil free operation. User-friendly software can store up to 60 programs under user-defined names within six sub-directories.

B PxE Thermal Cyclerl96-well format for flexible sample

throughput lCompact design for economy of spaceThe PxE thermal cycler is a licensed 96-well PCR machine, developed to provide all fundamental features for economical thermal cycling. It has two sample formats: model 02662-10 has a 96-well fixed block that fits 0.2-mL tubes; model 02662-12 has a 96-well fixed block that fits 0.5-mL tubes. Both models feature a high-definition 1⁄4 VGA display. User-friendly screen provides detailed information about the system status in graphical or text format with a minimal amount of user intervention.

C Px2 Thermal CyclerlMultiple interchangeable block options

include gradient and in situ flat blocksThe Px2 thermal cycler is an advanced personal gradient option PCR machine. It is offered with a choice of six interchangeable blocks (order separately at right), including the latest gradient cycling format. Should your requirements change, you need only purchase additional blocks rather than a complete thermal cycler.

Order either 96 x 0.2-mL format (02662-22), 48 x 0.5-mL format (02662-24), or 384-well format (02662-26). The in situ flat block

DMultiBlock

system 02662-42

APCR Sprint

thermal cycler 02662-00

B PxE thermal

cycler 02662-10

Cat. no. For cycler model Block type Capacity PriceKH-02662-04 PCR Sprint Replacement block 24-well x 0.2 mL KH-02662-06 20-well x 0.5 mL KH-02662-22

Px2 Interchangeable block96-well x 0.2 mL

KH-02662-24 96-well x 0.5 mL KH-02662-26 384-well x 0.04 mL KH-02662-28 Px2 In situ flat block 4 microscope slides, 76 x 26 x 1 mm KH-02662-30 Px2 Gradient block 96-well x 0.2 mL KH-02662-32 96-well x 0.5 mL KH-02662-44 MBS 384-well satellite block 384-well x 0.04 mL KH-02662-46 MBS Gradient satellite block 96-well x 0.2 mL

Block temperature range: 4ºC to 99ºC Block heating rate: up to 3ºC/sec Block cooling rate: up to 2ºC/sec

Block uniformity: ±0.5ºC within 15 secHeated lid temperature range: 90ºC to 120ºCPower input: 110 VAC


Key Catalog number Description Capacity Dimensions

(W x H x D) Price

A KH-02662-00 PCR Sprint thermal cycler 24-well x 0.2 mL 7" x 9" x 7" KH-02662-02 20-well x 0.5 mL

B KH-02662-10 PxE thermal cycler 96-well x 0.2 mL 91⁄2" x 111⁄4" x 16" KH-02662-12 96-well x 0.5 mL

C KH-02662-20 Px2 thermal cycler — 91⁄2" x 11" x 151⁄2"

D KH-02662-40 MBS system 2 block thermal cycler w/PC 73⁄4" x 111⁄2" x 113⁄4" KH-02662-42 2 block thermal cycler w/laptop

format (02662-28) holds four microscope slides. The gradient block features outstanding well-to-well uniformity—ensuring reproducible results when conditions are transferred to standard protocols. Thermal gradient blocks are available in 0.2- and 0.5-mL formats.

D MultiBlock System (MBS)The MultiBlock system features a central computer with sophisticated software, which can be networked to up to 30 individual satellite blocks. Order MBS start-up kits with a choice of PC or laptop. Each kit includes any two MBS satellite block unit.

Satellite blocks are available in 95 x 0.2-mL gradient format (02662-46) and 384-well format (02662-44). Additional satellite blocks can be added, up to 30 units. The choice of the number and type of each block is completely flexible, giving you freedom to customize your PCR system.

The MBS controlling software (included in the system) has been specially designed for ease of use. Graphical options for editing and running protocols can be viewed. You can either control and program all blocks in unison or program each block independently. The graphical display provides clear second-by-second information about block temperature and experimental parameters.

PCR Thermal CyclersDesigned to offer performance, accuracy, and simplicity

CPx2 thermal

cycler 02662-20

Additional Blocks

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Endurance Thermal CyclersReliable, high-quality resultsl Excellent block temperature uniformity—

typically ±0.3°C at 50°Cl Modern, intuitive programming and ready-to-go templatesl Selectable-temperature heated lidAll models feature variable voltage selector, PC control and networking connection, and password protection against unwanted program modifications. Adjustable-heated lid allows you to define pressure, program temperature from 100 to 115°C to accommodate a variety of consumables.

A Personal CyclersThis low-cost model is available as a stand-alone unit or can be PC-controlled in 20 x 0.5-mL or 25 x 0.2-mL microtube interchangeable block formats.

B High Sample Throughput CyclersThis high-performance model features a user-friendly, fully interchangeable block system that allows quick block exchanges (60 x 0.5-mL, 96 x 0.2-mL or 96-well plates, 384-well or flat plate for in situ). The large 41⁄2" x 31⁄2", 4-line display with intuitive simple-to-use software has alphanumeric programming, password protection, and simple copy/edit functions.

C Gradient CyclersThis model features a wide linear gradient with the range of 30°C. Protocols can be optimized in a single experiment. The unique “real-time” graphical display with user-friendly touch screen shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient (20 to 70°C). Gradient calculator function displays the temperature of each column of tubes. Optional SmartMedia memory card 93943-42 provides an alternative to internal memory storage of your programs, offering security and flexibility. This model also features the same versatile interchangeable blocks as the high sample throughput cycler.


AccessoriesKH-93943-42 Memory card for gradient cyclers

Catalognumber Capacity Block uniformity

at 50°CDimensions(W x H x D)

Maximumhold time

Minimumhold time Price

Additional interchangeable blocksCatalog number Price

A Personal cyclersKH-93943-02 20 x 0.5 mL <±0.1°C 611⁄16" x 71⁄2" x 13" 18 hrs 1 sec KH-93943-04 KH-93943-00 25 x 0.2 mL KH-93943-06

B High sample throughput cyclersKH-93943-08 60 x 0.5 mL

<±0.3°C 85⁄8" x 103⁄16" x 161⁄2" 18 hrs 1 sec

KH-93943-18 KH-93943-10 96 x 0.2 mL or 96-well plate KH-93943-20 KH-93943-12 384-well microplate KH-93943-22 KH-93943-14 Flat plate for in situ KH-93943-24

C Gradient cyclersKH-93943-28 60 x 0.5 mL

<±0.3°C 85⁄8" x 103⁄16" x 161⁄2" 99 hrs 1 sec

KH-93943-36 KH-93943-26 96 x 0.2 mL or 96-well plate KH-93943-34 KH-93943-32 384-well microplate KH-93943-40 KH-93943-30 Flat plate for in situ (non-gradient) KH-93943-38

Temperature range: 4 to 99°CTemperature set point precision: 0.1°CGradient range: 20 to 70°CMaximum gradient: 30°C (16°C for 384-well block)Variable programmable ramp rate: 0.1°C/sec stepsSelectable heated lid temperature*: 100 to 115°CDisplay

Models 93943-02 through -14: 4-line alphanumeric Models 93943-28 through -30: 41⁄2" x 31⁄2" touch screen, real-time graph

Number of programs Models 93943-02 through -14: 80 Models 93943-28 through -30: 50, or 500 using optional memory card

Serial port Models 93943-02 through -14: RS-232 Models 93943-28 through -30: RS-232/485

Power: 115/230 VAC dual voltage, 50/60 Hz

*Lid only heats up if block is set above 35°C.

A93943-02

B93943-08

C93943-28

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Thin-wall plate 67103-65

Individual tube 67103-90

PCR Tubes and PlatesDesigned to amplify nucleic acids during the PCR processl Precision molded from 99.9% pure virgin polypropylene—

withstand temperatures of –20 to 122°C and are autoclavablel Ultra-thin walls accelerate heat transfer providing shorter

cycle timesl Full-length, even insertion cap ensures a tight fit, eliminating

sample evaporationl Certified RNase, DNase, and pyrogen safe to ensure

no degradation of the target or amplified productl Choose from individual and strip tubes, and 96- and

384-well plates

PCR Sealing FilmPCR sealing film is specifically designed for PCR applications and the sealing of microplates for transport. The clear, 2-mL pressure-resistant polypropylene film eliminates well-to-well contamination and is RNase and DNase safe.

KH-67103-55 PCR sealing film. Pack of 100 sheets

Catalognumber Description Max well

volumeQty/pk

Price/pk

Individual PCR tubesKH-67103-90 Thin-wall with dome caps 0.2 mL

1000

KH-67103-60 Thin-wall without caps 0.2 mL KH-67104-60 Thin-wall with flat caps 0.5 mL

Thin-wall PCR strip tubesKH-67103-70 Thin-wall 8-tube strips 0.2 mL 125 KH-67103-75 8-strip caps —

Thin-wall PCR platesKH-67103-65 96-well, unskirted 0.2 mL

50

KH-67103-67 96-well, half skirt 0.2 mL KH-67103-66 96-well, full skirt 0.2 mL KH-67103-95 384-well, unskirted 0.4 mL


8-strip caps 67103-75

Thin-wall 8-tube strip 67103-70

PurePak™ PCR tubes and PlatesMade of the highest grade of 100% virgin polypropylene for optimal clarityl Thin-walled tubes for optimum heat transfer, and fit your thermocyclerl Free of RNase, DNase, nucleic acids, ATP, and pyrogensThin-wall tubes and plates are precision-molded with premium, nonwettable polypropylene. All sterile tubes and plates are certified free of DNA contamination, RNase and DNase detection, pyrogen detection, Bioburden, PCR inhibition, ATP detection, and sterility verification. Stringent manufacturing and QA/QC testing procedures ensure you and your samples the most aseptic products on the market. Exclusive PurePak packaging keeps tubes contamination-free. Ten individually sealed, puncture-proof bags with a zip closure for easy resealing.

Key Catalog number Description Max well volume Qty/pk Qty/cs Price/csIndividual thin-wall PCR tubesA KH-25715-10 Thin-wall with flat caps 0.2 mL 100 1000 B KH-25715-12 Thin-wall capless 0.2 mL 100 1000 C KH-25715-14 Thin-wall with dome caps 0.2 mL 100 1000 D KH-25715-16 Thin-wall with flat caps 0.5 mL 100 1000 E KH-25715-18 Thin-wall with dome caps 0.5 mL 100 1000

PCR strip tubesF KH-25715-20 8-tube strip with caps 0.2 mL 12 120 G KH-25715-22 12-tube strip with caps 0.2 mL 8 80

PCR platesH KH-25715-28 24-well thin wall plate 0.2 mL 1 25 H KH-25715-30 32-well thin wall plate 0.2 mL 1 25 H KH-25715-32 48-well thin wall plate 0.2 mL 1 25 H KH-25715-34 96-well thin wall plate 0.2 mL 1 25 I KH-25715-24 96-well skirted plate* 0.2 mL 50 50 J KH-25715-26 96-well semi-skirted* 0.2 mL 50 50

Caps and sealing tapes

K

KH-25715-36 8-strip dome caps 0.2 mL 12 120 KH-25715-38 8-strip flat caps 0.2 mL 12 120 KH-25715-40 12-strip dome caps 0.2 mL 8 80 KH-25715-42 Adhesive plate sealing tape — 100 100


* Nonsterile

Individual thin-wall PCR tubes

PCR strip tubes

PCR plates and caps

F G

H I J

A B C D E

K

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HotStart® Storage and Reaction TubesOptimize the hot start reaction and eliminate the need for messy oil overlays l Perform HotStart PCR with speed and simplicityl Thin-wall tubes feature a pre-positioned wax bead inside each tubel Conveniently store pre-aliquoted master mixes for PCR and sequencingl Complete the RT-PCR process in one tube The HotStart tubes automate the HotStart PCR process by using a pre-positioned wax barrier to separate key reagents until the annealing temperature is reached, thereby decreasing the occurrence of misprimes, primer-dimers, and premature annealing.. Tubes can also be used for the storage of commonly used primer sets and PCR product for months for later use or analysis. Simply create a master mix, aliquot into the tubes, melt the wax bead, and freeze! This is a great way to save time and ensure test-to-test consistency when regularly running several of the same reaction types using identical primer sets.

HotStart’s unique design can also facilitate a one-tube RT-PCR procedure by using a thermostable reverse transcription enzyme. Simply add your reverse transcription reagents to the lower layer and the PCR reagents to the upper layer. The first denotation cycle of PCR will melt the wax, denature any side reagents from the RT reaction, and begin the amplification of the target cDNA.

What's included: each pack includes tubes or strip tubes for 96 reactions.

Catalog number Tube capacity Reaction volume Price/pkKH-25715-50 0.2-mL tubes 15 to 25 µL KH-25715-52 0.2-mL 8-tube strips* 15 to 25 µL KH-25715-54 0.2-mL tubes 25 to 50 µL KH-25715-56 0.2-mL 8-tube strips* 25 to 50 µL KH-25715-58 0.2-mL tubes 60 to 100 µL KH-25715-60 0.5-mL tubes 25 to 50 µL KH-25715-62 0.5-mL tubes 60 to 100 µL


*Strip dome caps included.

EasyStart™ PCR Mix-In-A-TubeOne-step PCR with precisely formulated reagent mixl Fast, easy setup for contamination-free PCRl Enhanced performance with any standard Taql Room temperature storage EasyStart PCR tubes and strips have pre-aliquoted standard elements of PCR: dH2O, dNTPs, 10x buffer (200 mM Tris-HCl/500 mM KCl, pH 8.4), and MgCl2. The reagents are pre-assembled in a thin-wall PCR tube and hermetically sealed under a layer of wax to protect from oxidation and separating reagents from reaction-specific ingredients until the ideal reaction temperature is reached. EasyStart can be stored at room temperature for extended periods of time. It works with numerous DNA polymerase including enzymes from Boehringer-Mannheim, Perkin-Elmer, Invitrogen, and Promega. EasyStart products features one-step protocol, just add your preferred Taq DNA polymerase, template DNA, and primers to begin the reaction. EasyStart tube has a precisely formulated lower-layer reagent mix to ensure batch consistency and reproducibility.

What's included: each pack includes tubes or strip tubes for 96 reactions.


*Strip dome caps included.

Catalog number Tube capacity Reaction volume Price/pkKH-25715-70 0.2-mL tube 20 µL KH-25715-72 0.2-mL 8-tube strips* 20 µL KH-25715-74 0.2-mL tube 50 µL KH-25715-76 0.2-mL tube 100 µL KH-25715-78 0.5-mL tube 50 µL KH-25715-80 0.5-mL tube 100 µL

25715-50

ColeParmer.com/LifeScienceEven MORE online! Look for new items, stock status, technical info, etc.

Molecular Biology


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ThermalSeal™ Films for Thermal CyclingEliminate the need for heat-sealing devices or matsl Designed for PCR, cold storage, and real-time PCR l Certified free of DNase, RNase, and nucleic acidsThese heat-resistant, adhesive sealing films prevent evaporation and protect samples during thermal cycling applications. The polypropylene films (13024-12 and -14) offer sufficient sealing area for all PCR plates. Nonpermeable aluminum foil films (13024-16 and -18) feature a strong medical-grade adhesive for less roll back when removing the paper backing; also conform well to the plate shape. These films are easily pierceable with single or multichannel pipettors and robotic probes. Transparent polyester film (13024-20) is an optically clear film with a strong ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications.

Catalognumber Application Material Temperature

range Thickness Dimensions(W x L) Sterile Qty/

pkPrice/

pkKH-13024-12 PCR Heat-resistant polypropylene –40 to 125°C 50 µm 79.4 x 135.1 mm No

100

KH-13024-14 Yes KH-13024-16 PCR, cold storage Nonpermeable aluminum foil –80 to 120°C 38 µm 82.6 x 142.9 mm No KH-13024-18 Yes KH-13024-20 Real-time PCR Transparent polyester –40 to 120°C 125 µm 79.4 x 142.9 mm No

13024-12

13024-16 13024-20

Cloning DiscsSave time and effort when isolating a large number of coloniesSterile paper discs transfer cells quickly and easily to a 24-well plate for growth. Discs eliminate leak problems associated with cloning cylinders. Short exposure times reduce the risk of contamination and drying out. DNase and RNase free. Radiation sterilized.

Catalog number Diameter Qty/cs Price/csKH-13039-02 1⁄8" (3.2 mm)

100

KH-13039-04 3⁄16" (4.8 mm) KH-13039-06 1⁄4" (6.4 mm)


13039-06

Cloning Cylinders Convenient cloning technique ensures a homogenous cell populationCloning cylinders improve the isolation of individual cell clones, ensuring a homogenous population. Cylinders are molded of polystyrene and are tapered so the bottom is wider, ensuring a good sealing surface. Sterile. Supplied in three sizes; includes 40 of each size or order each size separately.

Catalog number Sizes (ID x H) Qty Price

KH-13039-003⁄16" x 5⁄16" (4.7 mm x 8 mm)1⁄4" x 5⁄16" (6.4 mm x 8 mm)3⁄8" x 1⁄4" (9.5 mm x 6.2 mm)

120/cs

KH-13039-10 3⁄16" x 5⁄16" (4.7 mm x 8 mm) 50/bx KH-13039-12 1⁄4" x 5⁄16" (6.4 mm x 8 mm) 50/bx KH-13039-14 3⁄8" x 1⁄4" (9.5 mm x 6.2 mm) 50/bx


13039-00

RNase AWAY® and DNA AWAY® Surface DecontaminantsThe fast, effective way to remove RNase and DNA contaminationThese surface decontaminants provide a safe, fast, and proven alternative to remove nuclease and DNA contamination from benchtops, instruments, pipettors, glass, and plasticware. Conventional methods to remove RNase and DNA contamination use a Diethylpyro- carbonate (DEPC) treatment, which is suspected to be carcinogenic and must be handled with gloves and under approved fume hoods at all time.

RNase AWAY leaves your instruments and other surface areas residue-free. You can spray, wipe, or soak your labware with RNase AWAY and remain confident your work is free of nuclease and nucleic acid contamination. It comes in 250-mL bottle or convenient 475- mL spray bottle. DNA AWAY requires no prep time and is ready to use straight out of the 250-mL bottle.

Catalog number Description Volume PriceKH-25715-00 RNase AWAY 250-mL bottle KH-25715-02 475-mL spray bottle KH-25715-04 DNA AWAY 250-mL bottle


25715-04

25715-00

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44175-06

Aluminum blocks are available for different sample tubes

Benchtop Chilling/Heating/Mixing BlocksChill, freeze, heat, or mix biological samples at your work stationl Store five programs in memory for precisely timed and

repeatable routinesl Choose blocks for centrifuge tubes, assay plates, PCR tubes

and plates, and morel Two-block unit offers two different or same temperaturesFeatures include a two-line LCD that reads actual and set point temperatures, 30-day countdown timer with alarm and auto/off, built-in data logger, and RS-232 I/O port for bidirectional communication with an IBM®-compatible computer. Energy-efficient Peltier design chills or heats biological samples from –10 to 100°C with ±1°C digital control.A Standard Chilling/Heating Block is ideal for applications such as storing enzymes or DNA libraries, maintaining 14°C for ligation reactions, and incubating or deactivating enzyme reactions.B Orbital Mixing Chilling/Heating Block combines shaking, heating, and cooling all in one instrument with a built-in orbital mixer with speeds settable from 200 to 1000 rpm.C Programmable Orbital Mixing Chilling/Heating Block is fully programmable with built-in orbital mixer. This model can store five programs in memory of as many as 10 steps per program where each step can be a temperature, time, and mixing speed. Each program can be repeated from 1 to 99 times automatically.D Two-Position Chilling/Heating Block is non-programmable. It features two individually controlled blocks that can be set to the same temperature or two different temperatures.

What's included: chiller/heater module, power cord, and a 12 VDC power supply. Order cover and aluminum blocks separately.

KH-44175-40 Cover, plastic; for 96-well assay plates .......................... KH-44175-50 Cover, plastic; for aluminum blocks below ...................


Key Catalog number Description Power PriceA KH-44175-00 Standard 100 to 230 VAC B KH-44175-02 Orbital 100 to 230 VAC C KH-44175-04 Programmable 100 to 230 VAC D KH-44175-06 Two-position 100 VAC

Interchangeable Aluminum Blocks are ideal for chilling or heating your samples. Measure 43⁄8"L x 27⁄8"W.

Catalog number Type Capacity PriceKH-44175-15 Micro

centrifuge tubes

40 x 0.5 mL KH-44175-20 30 x 1.5 mL KH-44175-35 40 x 0.5 mL and 15 x 1.5 mL KH-44175-30 PCR plates/strips 96 x 0.2 mL KH-44175-36 Flat bottom 96/384-well assay plates KH-44175-37 Test tubes 12 x 15/16-mm ID KH-44175-38 Test tubes 20 x 12/13-mm ID

Thermomixer Versatile—combines shaking, heating, and cooling in one instrument

Mixing speed 0.5-mL thermoblock: 300 to 1500 rpm All other thermoblocks: 300 to 1400 rpm

Mixing stroke: 3 mmTemperature range: 13°C below ambient

to 99°C, in 1° incrementsTemperature accuracy

From 20 to 45°C: ±0.5°C Below ambient and above 45°C: ±2°C

Heating rate: 5°C/minuteCooling rate

Above ambient: 2 to 3°C/minute Below ambient: 0.5 to 1°C/minute

Timer range: 1 minute to 99 hours, 59 minutes or continuous

Dimensions: 83⁄4"W x 43⁄4"H x 97⁄8"D

Catalog number Power PriceKH-03145-00 115 VAC, 50/60 Hz KH-03145-05 220 VAC, 50/60 Hz


AccessoriesThermoblocks are interchangeable—choose from blocks that hold microcentrifuge tubes or a microplate. The thermomixer automatically recognizes the thermoblock and displays the corresponding parameters. Each block includes an autoclavable, freezer-safe work rack and 0°C cooling pack to keep samples at 0°C for up to three hours on your lab bench. Plate adapter 03145-58 allows microplate thermoblock 03145-56 to hold 96 x 0.2-mL PCR tubes. Order microcentrifuge tubes on page 218.

KH-03145-58 Plate adapter for PCR tubes

Catalog number Capacity PriceKH-03145-50 24 x 0.5-mL microcentrifuge tubes KH-03145-52 24 x 1.5-mL microcentrifuge tubes KH-03145-54 24 x 2.0-mL microcentrifuge tubes KH-03145-56 One microplate

l Store two programs in memory—ideal for common or successive program

l Four interchangeable blocks allow you to use a variety of tube sizes— even a microplate

l Use short mix function for quick vortex mixing

Reduce processing time and your workload by mixing and incubating samples simultaneously. Program intermittent mixing with resting times for enzymatic reactions or DNA hybridizations— no need to watch over the process. Wide temperature range allows use with a variety of applications, including low-temperature applications such as DNA ligations. View actual and set operating parameters on digital display, then document with RS-232 interface.

44175-00

Thermomixer 03145-00 with thermoblock 03145-52

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Rotisserie Hybridizing Apparatus Economical, compact, and convenient l Economically convert your

existing incubator into a rotisserie hybridizing incubator or use for room temperature hybridization

l Place your bottles in the angled holders for tri-directional rotation— ensures optimal distribution of hybridizing materials

What’s included: a power cord with a three-prong plug.

Temperature range: 4 to 65°CRoller speed: 8 rpm

Construction: aluminum, PP, Delrin®

Power wattage: 3 watts


Minidizer™ Hybridization IncubatorCompact and portable desktop unit with small laboratory footprintl Economical design for

all your hybridization applications

l Personal desktop unit for low-throughput use

Catalognumber Capacity Dimensions

(W x H x D)Power

(VAC, Hz) Price

Small rotisserie hybridizing apparatusKH-39452-10 Four 35- to 38-mm x 150 mm bottles,

four 50-mL conical tubes 101⁄2" x 7" x 5" 120, 60 KH-39452-15 230, 50 Large rotisserie hybridizing apparatusKH-39452-20 Four 35- to 38-mm x 300 mm bottles,

eight 50-mL conical tubes 181⁄2" x 7" x 5" 120, 60 KH-39452-25 230, 50

39452-10

AccessoriesKH-39453-20 Glass hybridization bottle, 35 x 150 mm KH-17400-24 Corning conical bottom plastic tube, 15 mL KH-17400-30 Corning conical bottom plastic tube, 50 mL

Specifications & Ordering InformationTemperature range: ambient to 78°C Heating element: 165 watts

Temperature display: LEDRotation speed: 12 rpm

Catalognumber Capacity Dimensions

(W x H x D)Power

(VAC, Hz) Price

KH-39453-10 Four 35 x 150 mm bottles, four 50-mL tubes, eight 15-mL tubes

9" x 13" x 8" 115, 60

KH-39453-12 230, 50

This Minidizer™ incubator is ideal for use in laboratories with low usage and space requirements. Consistent, repeatable results are achieved with microprocessor controls. The chamber is stainless steel with a 3⁄8" thick ß-blocking acrylic cover. The cover swings down for access to the carousel. Pop the carousel out of the chamber for easy insertion or removal of bottles. Hybridization bottles are made of thick walled borosilicate glass, which helps protect users from radiation and has excellent long term reliability.

What’s included: carousel. Order bottles separately below.

39453-10

HybriCycler Hybridization IncubatorsPrecise temperature control, ambient to 80°Cl Bottle carousel rotates at a consistent 11 rpm enabling reliable saturation of samplesl Microprocessor controlled operation for precise mixing capabilityl Small laboratory footprint saves valuable spaceThis hybridization incubator creates the ideal environment for nucleic acid hybridization. Unique circular design enables complete visibility of bottles within the beta-blocking acrylic chamber. Both models operate with microprocessor controls to monitor temperature and rotation speed of bottles for dynamic mixing capability—ideal for creating the optimal conditions needed for cDNA library screenings, primer synthesis, and all nucleic acid hybridization. The system is easy to operate with the touch-sensitive key pad. To accommodate larger bottles, simply remove the carousel and place the bottle on the roller.

What's included: bottle carousel and pull-out spill tray. Order bottles separately below.

Catalog number Capacity Dimensions

(W x H x D)Power

(VAC, Hz) Price

KH-39453-06 Four 35 x 300 mm bottles, eight 35 x 150 mm bottles, eight 50-mL tubes

101⁄2" x 16" x 20" 115, 60 KH-39453-08 101⁄2" x 16" x 20" 230, 50

Specifications & Ordering InformationTemperature range: ambient to 80°C Temperature display: LED Rotation speed: 12 rpm Accessories

KH-39461-52 Bottle; small, 35 x 150 mm KH-39461-54 Bottle; large, 35 x 300 mm

Use hybridizing apparatus with incubators that contain internal electric outlets.

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l Ideal for hybridization of transfer membranes used in Southern and Northern blotting techniques

l Each removable tray holds up to one 20 x 20 cm blot

l PID temperature controller allows rapid heating and temperature stability

Removable shelves accommodate different size containers and heat sealed plastic bags. Maximum shelf load is 2.2 lb (1 kg).

What's included: three shelves, three removable trays, and 8-ft power cord with plug (three-prong plug on 115 VAC models; European plug on 230 VAC models).

Specifications & Ordering InformationCapacity: 0.8 cu ftTemperature range: 5° above ambient to 70°CDisplay: 3-digit LED, 3⁄8"HResolution: 0.1° CNumber of shelf positions: threeRoller speed: 8 to 25 strokes/minuteTray dimensions: 8"L x 81⁄2"W x 1⁄2"H Chamber dimensions: 111⁄2"L x 111⁄2"W x 101⁄2"HOverall dimensions: 141⁄2"L x 121⁄2"W x 16"H

Cat. no. Power PriceKH-39450-10 115 VAC, 50/60 Hz, 200 watts KH-39450-15 230 VAC, 50/60 Hz, 230 watts

Shaking Hybridizing IncubatorAdjustable stroke amplitude and speed—vary speeds from 8 to 25 strokes/min

39450-10

Accessories for 39457-00, -05, -10, and -15

KH-13247-16 Long-tube rotisserie, holds two 70 x 300-mm tubes KH-13247-18 Mounting bracket. Mount incubator under a cabinetKH-13247-19 Stacking plate. Stack up to two incubators KH-13247-15 Replacement rotisserie, holds four 35 x 300-mm tubes or eight 35 x 150-mm tubes KH-13247-17 Replacement tray, SS

Accessories for 39457-20, -25, -30, and -35

KH-13247-26 Long-tube rotisserie, holds four 70 x 300-mm tubes KH-13247-29 Stacking plate. Stack up to two incubatorsKH-13247-25 Replacement rotisserie, holds ten 35 x 300-mm tubes or twenty 35 x 150-mm tubes KH-13247-27 Replacement tray, SS

Rocker Platform Assembly rocks large sample containers or pre-heats bags of buffer. Gentle back-and-forth motion allows even heat distribution, keeping samples homogenized. Rocker speed is variable, <5 to 15 rpm (±1 rpm).KH-13247-28 Rocker platform assembly

Hybridization IncubatorsProgrammable temperature range from 7°C above ambient to 85°Cl Microprocessor-based PID controller guides you through

the programming process on digital displayl Forced-draft convection provides temperature uniformity l Eliminate the need for water baths and heat sealers—ideal for nucleic acid

and protein hybridizationThe variable-speed rotisserie accommodates standard 35-mm ID tubes, plus the incubators accepts optional rotisseries to hold 70 x 300-mm tubes. Touch pad simplifies temperature selection. Tube grips hold tubes securely and are kind to your hands. Incubators feature manual offset calibration. Use the optional stacking plate to stack two incubators, saving valuable space.

What’s included: rotisserie, drip pan, and cord with plug (see table for type of plug).

39457-00Temperature range: 7° above ambient to 85°CResolution: 0.1°CUniformity

Chamber: ±0.5 at 55°C Sample-to-sample: ±0.25°C

Rotisserie speed: variable from <5 to 15 rpm, ±1 rpm

Rise rate: ambient to 65°C in 20 minutes; two-minute recovery at 37°C after door is open for 30 seconds

Over-temperature cutoff: yesMaterials of construction: stainless steel interior

and drip tray, tempered low-beta-emission glass window, painted steel exterior

Cat. no. Tube size (ID x L) Qty/pk Price/pkKH-13247-75 35 x 75 mm 2 KH-13247-10 35 x 100 mm 2 KH-13247-45 35 x 150 mm 2 KH-13247-35 35 x 225 mm 2 KH-13247-30 35 x 300 mm 2 KH-13247-70 70 x 300 mm 1

Hybridization TubesBorosilicate glass hybridization tubes fit most rotisseries. The 35-mm ID tubes come with a polypropylene cap and silicone O-ring. The 70-mm ID tube comes with a lined cap.

KH-13247-50 Replacement O-ring, for 35-mm ID hybridization tubes

120 VAC modelsSpecifications & Ordering Information

Cat. no. Capacity Dimensions (W x H x D) Power Plug type PriceKH-39457-00

Eight 35 x 150-mm tubes,four 35 x 300-mm tubes

Chamber: 15" x 91⁄2" x 8"Exterior: 18" x 171⁄8" x 161⁄2"

Window: 14" x 9"

120 VAC, 60 Hz U.S. Std KH-39457-05 230 VAC, 50/60 Hz European KH-39457-10 100 VAC, 50/60 Hz Japanese KH-39457-15 230 VAC, 50/60 Hz British KH-39457-20

Twenty 35 x 150-mm tubes,ten 35 x 150-mm tubes

Chamber: 15" x 13" x 12"Exterior: 18" x 201⁄2" x 20"

Window: 14" x 12"

120 VAC, 60 Hz U.S. Std KH-39457-25 230 VAC, 50/60 Hz European KH-39457-30 100 VAC, 50/60 Hz Japanese KH-39457-35 230 VAC, 50/60 Hz British

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KH-39453-18 Oversize bottle carousel kit KH-39453-20 Glass hybridization bottle, 35 x150 mm KH-39453-22 Glass hybridization bottle, 25 x 300 mm KH-13247-70 Glass hybridization bottle, 70 x 300 mm

39453-14

Catalog number Capacity Dimensions (W x H x D) Power (VAC, Hz) Price

KH-39453-14 Twenty 35 x 150-mm bottles, ten 35 x 300-mm bottles, four 70 x 300-mm bottles

Exterior: 171⁄2" x 24" x 15"Hybridizer interior: 14" x 103⁄4" x 103⁄4" Crosslinker interior: 11" x 33⁄4" x 111⁄2"

115, 60

KH-39453-16 230, 50

Specifications & Ordering InformationTemperature range: –9.9 to 99.9°C

HybriLinker™ Hybridization IncubatorHybridizer and UV crosslinker in one self-contained space-saving unitl Designed for effective, efficient crosslinking and hybridization at one location with each

section able to operate at the same timel Precise temperature control, ambient to 99.9°C and variable speed control at 10 to 18 rpml Multiple bottle sizes fits up to ten bottles with three different-sized configurationsThis microprocessor-controlled hybridization oven monitors temperature and rotation speed of bottles for dynamic mixing capability. This is ideal for creating the optimum conditions needed for cDNA library screenings, primer synthesis and all nucleic acid hybridization. The UV crosslinker provides an extremely uniform shortwave radiation exposure chamber for bonding nucleic acid to medium, which is typically done prior to hybridization. UV output is measured by an internal sensor and controlled by an on-board microprocessor, enabling accurate, reliable and safe laboratory crosslinking. Preset or user-selected UV energy or UV time exposure are selected via touch pad.

What’s included: bottle carousel and one large 35 x 300-mm bottle.

UV CrosslinkersMicroprocessor-controlled UV sensor feedback system ensures maximum energy efficiencyl Available with interchangeable shortwave, midrange, and longwave tubesl Preset and manual controls for UV or time exposure settingsl Small footprint saves valuable benchtop spaceUV crosslinking procedure optimizes the use of the UV energy while ensuring that crosslinking is consistent. Internal UV sensor is calibrated with traceability to NIST (or NPL in UK and Europe) standards. Change the wavelength for different applications by changing the UV tube (order separately) and corresponding UV wavelength calibration sensor. Other features include preset or user-selected UV exposure and time, internal safety system with automatic shutoff, large LED that displays remaining time or energy, and a UV-blocking clear viewing window. The 115 VAC model is ETL/ETLc certified, and the 230 VAC model is CE certified.

Use shortwave (254 nm) for sterilization, uniform radiation for crosslinking DNA or RNA to membranes after Northern or Southern blots, dot blotting, and colony or plaque lifts. Other applications include nicking ethidium bromide stained DNA in agarose gels and gene mapping for creating cleavage-inhibiting thymine dimers. Use midrange (302 nm) for phototherapy, UV crosslinking and curing, and gradient sampling. Use longwave (365 nm) for UV curing, photochemistry, and immunology research.

What's included: 8-ft power cord with plug (three-prong plug on 115 VAC models; standard European plug on 230 VAC models).

Catalog number Wavelength (nm) Power (VAC, Hz) PriceReplacement tubes

Catalog number PriceKH-39462-00* 254 115, 60 KH-97607-50 KH-39462-05* 230, 50 KH-39462-10†

254 115, 60 KH-97607-50 KH-39462-12† 230, 50 KH-39462-18 302 115, 60 KH-97608-58 KH-39462-20 230, 50 KH-39462-14 365 115, 60 KH-97607-52 KH-39462-16 230, 50

Specifications & Ordering InformationDimensions

Chamber: 10"W x 5"H x 12"D Overall: 155⁄8"W x 83⁄4"H x 133⁄4"D

*With swing-out door †With pull-down drawer

39462-00

39462-10

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AccessoriesKH-13099-60 PC interface software with cableKH-13099-62 Advanced data analysis softwareKH-13099-64 Adapter for 20-mL LSC vial KH-13099-66 Adapter for 7-mL LSC minivials KH-13099-68 Adapter for microcentrifuge tubes KH-13099-70 Plastic scintillator for 32P counting KH-13099-72 Manual syringe lid for luminescenceKH-07938-15 Standard Microliter™ syringeKH-13099-56 Internal lead shieldKH-13099-50 Field case KH-13099-52 Field case with battery KH-13099-54 Power cord with automotive adapter for cigarette lighter KH-13099-58 Replacement power supply, 100 to 240 VAC, 50/60 Hz


Catalog number Cap material/liner Cap size Caps Qty/cs Price/csEK-08918-01 Urea/metal foil

22 On 500

EK-08918-02 Urea/Polyseal® cone EK-08918-03 Urea/polyethylene disc EK-08918-05 Urea/polyethylene disc 24 On 500 EK-08918-06 Polypropylene/metal foil 22

Liquid Scintillation Vials and Support

A 20-mL Glass Scintillation Vials are precision formed from chemical-resistant Wheaton “180” borosilicate glass. Vials are available in four cap/liner styles and are supplied with caps on. They measure 23⁄8"H x 11⁄8" OD (61 mm H x 28 mm OD) with cap.

B 20-mL Polyethylene Scintillation Vials are available in four cap/liner styles. Order with caps on, or bulk packed separately. Lightweight and durable polypropylene vials measure 23⁄8"H x 11⁄16" OD (61 mm H x 27 mm OD) with cap.

Catalog number Cap material/liner Cap size Caps Qty/pk Price/pkEK-08918-51 Urea/metal foil

22 On 500

EK-08918-52 Polyethylene/linerless EK-08918-54 Polypropylene/metal foil EK-08918-63 Urea/Polyseal cone

22 Bulk 1000

EK-08918-62 Polyethylene/linerless EK-08918-53 Polypropylene/metal foil

C 7-mL MiniVial® Scintillation Vials reduce costs and minimize waste by reducing the amount of solvent required. High-density polyethylene vials have a 7-mL capacity, but are more efficient with 5 mL of solution. Vials measure 21⁄16"H x 5⁄8" OD (53 mm H x 16 mm OD) with cap.

Catalog number Description Qty/pk Price/pk*KH-08980-00 MiniVials, 7 mL 1000

*Discounts: Save 10% on 2-9 pks, 15% on 10-39 pks, 20% on 40 pks or more.

†Discounts: Save 10% on 4-19, 15% on 20-79, and 20% on 80 supports or more.

Detector: photon counting photomultiplier tube

Energy range: 2 to 2000 keVDynamic range: 200 to

50,000,000 CPSCounting time: 0.1 second to

99,999 minutes

Counting efficiency: (gamma counter) 70% for 125I

Output: RS-232 Power: 110 to 240 VAC, 50/60 Hz

or 12 VDCDimensions: 13"L x 10"W x 71⁄2"H

Catalog number Description PriceKH-13099-00 Liquid scintillation and luminescence counter KH-13099-02 Gamma counter

KH-13099-04 Multilabel counter: liquid scintillation, luminescence counter, and gamma counter

13099-00

Triathler™ Single-Sample CountersLiquid scintillation/luminescence or Gamma counting at economical prices

Vial support 06729-00 shown with vials 08918-0508918-01 08918-63 08980-00

l Easy-to-use, single key operation— press a button and start counting

l Compact size and light weight make counters ideal for laboratory or field use

l Accept almost any type and size of vial or microcentrifuge tubeThese flexible systems adapt easily for different protocols. All counters have preprogrammed methods for most isotopes and luminescence. Features include built-in data reduction and calibration, printer output and RS-232 interface. Order optional software for advanced spectrum analysis, data export to spread sheet programs and full instrument control.

Liquid Scintillation (LSC) and Luminescence CounterSample counter has programmable channel selection for 3H, 14C, 35S, 32P, and other isotopes up to 2000 KeV and a preset mode for “glow” luminescence detection. Maximum count rate is 2,000,000 cpm for liquid scintillation and 3 x 107 for luminescence.

Use plastic scintillator 13099-70 for 32P counting; order separately below right. An optional lead shield is available for low background applications. Counter includes an adapter for 20-mL vials; order vials at right. Adapters for 7-mL vials and microcentrifuge tubes are also available; order at right. For luminescence counting, order the manual syringe lid and Microliter™ syringe below right.

Gamma CounterThis counter has preset modes for 57Co, 51Cr, 131I and 99mTC. Program gamma isotopes up to 1000 keV. Features built-in, 10-mm lead background shield and 32 mm x 32 mm NaI (TI) crystal detector. Accommodates tubes or vials up to 13 mm in diameter.

A B CD

Catalog number Description Price/pk†

KH-06729-00 Scintillation vial support

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Picofluor™ Handheld Dual Channel FluorometerAchieve the sensitivity, linearity, and reproducibility expected from full-sized instruments!l Compact size saves valuable bench spacel Two dedicated optical channels let you measure two

different fluorophores in the same assayl Store 1000 data points

Detector: photodiodeCalibration: single-point and blankPower: four AAA batteries (included)

Operating temperature: 5 to 40°CDimensions: 71⁄4"L x 31⁄2"W x 13⁄4"H

Accessories KH-13095-50 Cuvette; square, polystyrene, 10 x 10 mm. Pack of 100KH-13095-52 Cuvette; square, methacrylate, 10 x 10 mm. Pack of 100KH-13095-54 Minicell adapter kit, includes 500 cuvettes KH-13095-56 Cuvettes; minicell, borosilicate glass. Pack of 500KH-06755-00 Cuvette rack. twelve 10-mm pathlength square cuvettes. Measures 45⁄8"L x 41⁄4"W x 43⁄8"H KH-06755-20 Cuvette holder. Holds twelve 10-mm pathlength square cuvettes without obstructing tops. Measures 63⁄4"L x 2"W x 1⁄2"H. Pack of 5


Catalog number Channel Excitation Emission Price

KH-13095-00 Channel A (UV) 365 nm to 395 nm >430 nm Channel B (Blue) 475 nm ±15 nm 515 ±10 nm

KH-13095-02 Channel A (Green) 540 nm ±20 nm >570 nm Channel B (Blue) 475 nm ±5 nm 515 ±10 nm

13095-00

Portable meter measures as little as 1 ng/mL

double-stranded DNA

This battery-operated fluorometer features dual optical channels to quickly analyze samples for two different fluorescent signals. Quantify probes for nucleic acids, proteins, metabolites, and other fluorescent reporters. Measure as little as 1 ng/mL of double stranded DNA and 2.5 ng/mL of RNA. This unit has a four-decade dynamic range and built-in datalogging with interfacing software to Microsoft® Excel. Order standard cuvettes for volumes up to 3.5 mL; order the minicell adapter kit and minicell cuvettes for volumes between 70 to 250 mL.

What’s included: four AAA batteries.

Small and portable, the luminometer uses a light reflector to produce excellent sensitivity within the entire six decades of dynamic range. Operation is simple: insert the sample and close the sliding sample door—the measurement sequence begins automatically. The luminometer accepts samples in 12 mm diameter test tubes with lengths ranging from 45 mm to 100 mm and 15 mL microcentrifuge tubes. The built-in software provides complete sample documentation and transferring of data to a PC; export results to Microsoft® Excel for further analysis.

Use the optional protocol software to transfer data to a PC and export to Microsoft® Excel. The optional software allows automation of all measurement steps, from defining parameters to reporting final results. Protocols exist for quick measurement, single and dual assay, cut-off assay, and real-time viewing of single and multiple kinetic measurements or create user-defined protocols. Order individual protocols or a six protocol package below.

What’s included: software, interface cable, and power supply.

Single-Tube LuminometerHigh sensitivity with low background noise and a wide dynamic rangel Compact and portable—ideal for on-site testing l Stores up to 800 measurements

Specifications & Ordering InformationDetector: photomultiplier tube with

bialkali cathode and a spectral range of 370 nm to 630 nm

Sensitivity: 20 attomoles ATPDynamic range: 6 decadesInterface: RS-232Measuring time: 1 sec to 999 sec;

or continuous

Power: rechargeable NiCd batteries, 12 V supplied through adapter (included)

Operating temperature: 10 to 35°CHumidity: 10% to 80% (non-condensing)Storage temperature: 0 to 40°CDimensions: 51⁄2"H x 63⁄4"W x 43⁄8"D

13098-30

AccessoriesKH-13098-50 Carrying case KH-13098-52 Protocol software, single protocol, specify which protocol when ordering KH-13098-54 Protocol software, six protocol package

Catalog number Description PriceKH-13098-30 Single-tube luminometer

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Catalognumber

Culturearea

Dish style

Workingvolume

Dimensions(mm) Surface Qty/

csPrice/

csKH-01835-00 8 cm2 Round 3 mL 10 H x 35 dia Treated 500 KH-01835-01 21 cm2 Round 6 mL 15 H x 60 dia Treated 500 KH-01835-08 Not treated KH-01835-03 55 cm2 Round 15 mL 20 H x 100 dia Treated 500 KH-01835-09 Not treated KH-01835-05 148 cm2 Square 30 to 45 mL 25 H x 150 dia Treated 60 KH-01835-06 500 cm2 Square 100 to 150 mL 245 L x 245 W x 25 H Treated 16

KH-01835-10 500 cm2 Square w/spillguard 100 to 150 mL 245 L x 245 W x 18 H Treated 16

Catalognumber

Culturearea

Necktype

Workingvolume

Totalvolume

Captype

Qty/cs

Price/cs

KH-01936-0025 cm2 Canted 10 mL 30 mL Plug seal 500

KH-01936-02 Vent 200 KH-01936-40

25 cm2 Angled 10 mL 30 mL Phenolic 500 KH-01936-41 Vent 200 KH-01936-10

75 cm2 Canted 60 mL 250 mLPlug seal

100

KH-01936-12 Phenolic KH-01936-14 Vent KH-01835-12

75 cm2 Straight 80 mL 200 mLPhenolic

100

KH-01835-14 Vented KH-01936-16 Plug seal KH-01936-20

150 cm2 Canted 175 mL 450 mL Plug seal 50 KH-01936-22 Vent KH-01936-30

175 cm2 Angled 250 mL 600 mLPhenolic 50

KH-01936-32 Vent KH-01936-34* 84

*Barcoded

LILife Science

Cell Culture DishesVented lid design provides consistent gas exchangel Optically clear for easily viewing cellsl 500-cm2 square culture dish with spillguardThese polystyrene cell culture dishes feature stacking beads for easier handling. All models are treated for optimal cell attachment (except models 01835-08 and -09). Models 01835-08 and -09 are ideal for applications where cell attachment is not required. All are sterilized by gamma radiation and certified nonpyrogenic. Model 01835-10 features removable spillguard to protect against media spillage during handling and transporting to incubator.

Specifications & Ordering InformationMaterial: polystyrene


Cell Culture FlasksAvailable in a variety of sizes, designs, and cap styles to meet your needsl Virgin polystyrene provides excellent optical qualityl Gamma radiated; certified nonpyrogenic and nontoxicl Barcoded model availableFlasks have individually printed lot numbers for product traceability. Feature molded-in graduations. Anti-tip skirt provides increase stability on 25-, 75-, and 150-cm2 flasks. Triangular flasks, models 01936-40 and 01936-41, offer increased stability and easy pipet access.

Plug seal cap features a one-piece linerless construction for both a gas and liquid-tight seal.

Phenolic cap is designed for open culture systems requiring gas exchange.

Vent cap features a 0.2-µm membrane sealed into the cap to permit sterile gas exchange when working with flasks in open cell culture systems.

Cap Types

Canted neckStraight neck Angled neck

Neck Types

01835-10

01835-05

01936-00

01936-34

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Catalog number Culture area Neck type Cap type Qty/cs Price/csKH-01936-50 25 cm2 Canted Phenolic 500 KH-01936-45

75 cm2 Canted Phenolic 100 KH-01936-46 Vented KH-01936-52 150 cm2 Canted Phenolic 50 KH-01936-54 175 cm2 Angled Plug 50 KH-01936-36 225 cm2 Canted Phenolic 24 KH-01936-38 Vented

Catalog number Culture area Neck type Cap type Qty/cs Price/csCell culture flasks

KH-01930-4125 cm2 Angled Vent/close 160

KH-01932-41 Filter 160 KH-01930-42

80 cm2Straight Vent/close 50

KH-01932-42 Filter 50 KH-01930-43 Angled Vent/close 50 KH-01932-43 Filter 50 KH-01930-44 175 cm2 Straight Vent/close 32 KH-01932-44 Filter 32

Easy Y flasks; wide angle opening allows full access to growth surface KH-01930-48*

25 cm2 Angled “Y” 200 KH-01930-53 Vent/close 200

KH-01930-49*75 cm2 Angled Filter 100

KH-01930-54 30 KH-01930-55 175 cm2 Angled Filter 30 KH-01930-56 “Y” Vent/close 30

SoLo flasks; expanded culture area in a low-profile designKH-01931-20 185 cm2 Angled Vent/close 50 KH-01931-22 Filter 50

LI Life Science


Cell Culture FlasksTreated for optimal cell attachmentl Individually printed lot numbers aid in product traceabilityl Optically clear polystyrene; sterilized by gamma radiation and certified nonpyrogenic

01936-46

*Quick 1/3-turn from vent to close.

SoLo flask 01931-20

Specifications & Ordering InformationFlask material: polystyrene

Cell Culture FlasksSurface treatment provides optimal cell attachment and growthl Virgin polystyrene provides excellent optical qualityl Sterilized by gamma radiationl Large-bore necks—easy pipette and cell scraper access

TripleFlasksThree parallel growth surfaces provide a total culture area of 500 cm2!These sterile polystyrene tissue culture flasks are treated and certified for consistent cell growth. Each flask has the same external dimensions of a 175 cm2 standard flask, however, the three parallel growth surfaces provide a total culture area of 500 cm2—ideal for large-scale tissue culture and monoclonal antibody production.

What’s included: extra sterile caps.

Specification & Ordering InformationFlask material: polystyreneCap material: high density polyethylene (HDPE)

Catalog number Surface treatment Culture

areaNeck type

Working volume Cap type Qty/pk Qty/cs Price/cs

KH-01931-00 Yes

500 cm2 Straight 200 mL

Vent/close

4 32

KH-01928-42 No Filter KH-01931-10 Yes Filter

KH-01928-46* Yes Filter *Barcoded flask

Surface-Treated PolystyreneManufactured from virgin polystyrene resin that has been specifically treated to ensure:l Optimal cell attachment and growthl Exceptional optical clarityl Certified nonpyrogenicl Adherence to the USP biological reactivity

class VI test



847


Catalog number Description Reservoir size Qty/cs Price/csKH-13052-10 CL350 350 mL 5 KH-13052-20 CL1000 1000 mL 3

Specification & Ordering InformationMaterial: polystyreneCap material: high-denisity polyethylene (HDPE)

Dishes

Catalog number

No. of wells

Working volume

Culture area

Well shape

Overalldimensions Qty/pk Qty/cs Price/cs

KH-01928-60 4 1 mL

500 cm2

Flat 66 x 66 mm 4 120 KH-01928-62 6 3 mL Flat 128 x 86 mm 1 75 KH-01928-64 12 2 mL Flat 128 x 86 mm 1 75 KH-01928-66 24 1 mL Flat 128 x 86 mm 1 75 KH-01928-68 48 0.5 mL Flat 128 x 86 mm 1 75

FlasksCatalog number

Culture area (cm2)

Neck type

Workingvolume

Totalvolume

Captype Qty/pk Qty/cs Price/cs

KH-01928-70 25 Angled 7 mL 70 mL

Filter

10 200 KH-01928-72 75 Angled 30 mL 260 mL 5 100 KH-01928-74 175 Angled 55 mL 645 mL 5 30 KH-01928-42 500 Straight 200 mL 700 mL 4 32

Specification & Ordering Information

Catalog number Blade width Blade offset Total length For flask size Qty/cs Price/csKH-01928-50 15.5 mm 7.5 mm 23 cm 25 to 80 mL 250 KH-01928-52 17.5 mm 16 mm 32 cm 75 to 175 mL 250

LILife Science

13052-10

01928-50 01928-52

13052-10

CELLine™ Cell Culture SystemsIdeal for monoclonal antibody and cellular protein production—viable in vitro alternative to using animalsl Patented design features separate growth and media chambers—provides continuous

supply of nutrients, direct oxygenation, and elimination of waste build up for high cell densities

INTEGRA CL350 5-mL Cell Cultivation Chamber is perfect for cultures requiring high cell densities. Use with a microscope for three-dimensional studies. Ideal for stem cell expansion, primary cell cultures, or antibody production.

INTEGRA CL1000 15-mL Cell Cultivation Chamber is ideal for small-scale production of monoclonal antibodies and recombinant protein expression. Accommodates over 108 cells/mL.


Non-Treated Polystyrene Culture Dishes and FlasksFor cells that do not need a treated surfacel Certified pyrogen-free and sterileThese sterile polystyrene tissue culture dishes and flasks are used for unique cell growth applications that do not require the Nunclon® surface. Non-treated surface offers low cell attachment. Clear, uniform design provides excellent optical quality, making it a suitable choice for microscopy.

Dishes have raised well rims to lower risk of cross contamination. Flasks have a white cap for easy identification. Flask 01928-42 offers a unique three parallel growth surface that provides a total culture area of 500 cm2—ideal for scale-up.

What’s included: lid for dishes and an extra sterile cap for non-treated flask.

Disposable Cell Scrapers, Sterile

Adjustable blade for optimal flexibilityl Soft blade for improved cell retrievall Sterile and nonpyrogenicl Available in two lengths



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LI Life Science

Canted neck

Plug seal cap features a one-piece linerless construction for both a gas and liquid-tight seal.

Phenolic cap is designed for open culture systems requiring gas exchange.

Vent cap features a 0.2-µm membrane sealed into the cap to permit sterile gas exchange when working with flasks in open cell culture systems.

Straight neck

Angled neck

Cap Types Neck TypesSpecifications & Ordering Information

Cell Culture Dishes, Flasks, and Plates with CellBIND® SurfaceA novel surface treatment for improved cell attachmentl Better cell attachment leads to increased cell growth and yieldsl May eliminate the need for tedious, time-consuming expensive and

low stability biological coatingsThe CellBIND surface enhances cell attachment under difficult conditions, such as reduced-serum or serum-free medium, resulting in higher cell yields. This patented technology uses a microwave process for treating the culture surface. This process improves cell attachment by incorporating significantly more oxygen into the cell culture surface, rendering it more hydrophilic (wettable) and increasing surface stability. The surface also reduces premature cell detachment from confluent cultures especially in roller bottles.

The CellBIND culture surface features the same high quality standards as other vessels. Culture inoculating and harvesting should be performed in the same manner as current methods. Both enzymatic and nonenzymatic dissociating solutions have been successfully used to remove cells from CellBIND surfaces. All products are individually printed with the CellBIND logo to differentiate from standard treatment cell culture products and to avoid mix-ups. Select from dishes with lids, flasks with caps, or plates with lids.

Corning CellBIND surface

Cell Scrapers and LifterScrapers and lifter are ideal for mechanically harvesting cells

The unique blade design of these cell scrapers minimizes cell damage and ensures even contact with the growth surface. The small scraper is designed for small- and medium-sized flasks; the large scraper fits larger flasks and roller bottles. The cell lifter is used with dishes and cell culture clusters. All are individually wrapped and irradiation sterilized.


Cell lifter 01835-50

Large scraper 01835-52

Small scraper 01835-51

Cell culture dishesCatalog number

Culture area

Dish style

Working volume Dimension (mm) Qty/

csPrice/

csKH-01835-60 8 cm2 3 mL 10 H x 35 dia 210 KH-01835-62 21 cm2 Round 6 mL 15 H x 60 dia 126 KH-01835-64 55 cm2 15 mL 20 H x 100 dia 40

Catalog number Description Blade width Handle length Qty/cs Price/csKH-01835-51 Small scraper 18 mm 250 mm KH-01835-52 Large scraper 30 mm 390 mm 100 KH-01835-50 Cell lifter 19 mm 180 mm

Cell culture flasksCatalog number

Culture area

Neck type

Working volume

Total volume

Cap type

Qty/cs

Price/ cs

KH-01835-30 25 cm2

Canted10 mL 30 mL

Vent200

KH-01835-32 75 cm2 60 mL 250 mL 100 KH-01835-34 150 cm2 175 mL 450 mL 50 KH-01835-36 175 cm2

Angled250 mL 600 mL Vent 50

KH-01835-72 175 cm2 250 mL 600 mL Phenolic 50 KH-01835-38 225 cm2 370 mL 1000 mL Vent 25

Cell culture platesCatalog number

No. of wells

Well diameter

Working volume

Culture area (cm2/well)

Qty/sleeve

Qty/cs

Price/ cs

KH-01835-66 6 34.8 mm 5.0 mL 9.5 KH-01835-68 12 22.1 mm 3.0 mL 3.8 5 50 KH-01835-70 24 15.6 mm 2.0 mL 1.9

ColeParmer.com/tissuecultureFor our latest selection of tissue and cell culture products.



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LILife Science

PS Cell Culture Dishes and Plates


96-Well MicroWell® Plates. Redesigned plates are sized and formatted for automated handling; plates measure 86 x 128 mm. Lids feature condensation rings that reduce evaporation and eliminate contamination. Plates with bar code are sequentially numbered with unique numbers using Code 39.

MicroWell® Plates 01930-13 Flat Round V-bottom/conical C-bottom

Rounded-square �(top view)


Multidish®. Select either round well or rectangular well styles—designed for convenient stacking and handling. Round well styles feature vented lids for gas exchange and recessed areas around wells that act as a water reservoir to reduce evaporation from wells. Dishes with 6-, 12-, 24-, and 48-well formats include lids with condensation rings to minimize evaporation. The 12- and 48-well dishes have a standard 96-well footprint. Rectangular well styles offer a larger culture area. They also accept standard glass cover slips: 24 x 60 mm for 4-well plate; and 22 x 22 mm for 8-well plate. Radiation sterilized.

Certified surface ensures optimum cell attachment and growth—use for scale up, cloning, viral titration, and fusion

*Model 01929-61 has a pinch bar design

01930-18 01930-17

OmniTraysCertified for consistent cell growthThe single-well, sterile polystyrene OmniTray is ideal for multiple applications. Choose the standard tray with notched corners or the rectangular tray for computerized imaging and spotting. Enhanced rectangular area is ideal for automated picking and viewing systems.

Specifications & Ordering InformationMaterial: polystyrene

Catalognumber Well style Working

volumeCulture area

(cm2/well) Lid Overall dimensions Qty/pk Qty/cs Price/cs

KH-01928-54 Notched corners, clear90 mL 84 Yes 128 x 86 mm 10

60 KH-01928-56 Rectangular, clear 90 KH-01928-58 Rectangular, white 90

Catalognumber

No. ofwells

Welldiameter

Workingwell volume

Culture area(cm2/well)

Overalldimensions

Qty/sleeve

Qty/cs

Price/cs

KH-01930-25 4 15.0 mm 0.4 to 1.0 mL 1.9 66 x 66 mm 4 120 KH-01930-19 6 35.0 mm 2.0 to 5.0 mL 9.6 88 x 132 mm 1 75 KH-01930-21 5 85 KH-01930-29 12 21.0 mm 0.7 to 1.8 mL 3.5 86 x 128 mm 1 75 KH-01930-23 24 15.0 mm 0.4 to 1.0 mL 1.9 88 x 132 mm 5 85 KH-01930-24 48 12.0 mm 0.3 to 0.6 mL 1.1 86 x 128 mm 1 75 KH-01930-17 4 24 x 67 mm 3.3 to 8.0 mL 16.0 32 x 132 mm 10 100 KH-01930-18 8 26 x 33 mm 2.0 to 4.5 mL 8.6 82 x 132 mm 10 100

Catalognumber

Wellshape

Workingwell volume

Culture area(cm2/well) Lid Bar code Qty/

sleeveQty/cs

Price/cs

KH-01930-13Flat 0.17 mL 0.33 Yes No

1 50 KH-01930-08 6 108 KH-01929-60 10 160 KH-01929-62 Flat 0.17 mL 0.33 Yes Yes 1 50 KH-01930-70 KH-01930-71 Flat 0.17 mL 0.33 No Yes 10 180 KH-01930-10 Round 0.17 mL 0.36 Yes No 1 50

KH-01929-61* 6 108

01928-54

l Tested for cell growth and plating efficiency to ensure consistent biological performance

lRaised well rims reduce risk of cross contamination

Multidishes®

Surface-Treated PolystyreneManufactured from virgin polystyrene resin that has been specifically treated to ensure:l Optimal cell attachment and growthl Exceptional optical clarityl Certified nonpyrogenicl Adherence to the USP biological reactivity class VI test



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LI Life Science

Cell Culture ClustersOptically clear, virgin polystyrene is treated for optimal cell attachmentl Stacking beads and uniform footprints (86 x 128 mm) facilitate stacking and handlingl Serrations on plate flange/skirt enable you to easily distinguish plates from lids

when handling stacks of clustersl Lids feature individual condensation rings to reduce well-to-well contaminationl Gamma radiation sterilized and certified pyrogen-free

01959-24

Costar® cell culture clusters set

Tissue Culture Dishes and Plates with Ultra-Low Attachment SurfaceUnique hydrogel surface inhibits cell attachmentThese culture plates and dishes have a unique covalently bonded hydrogel surface that is hydrophilc and neutrally charged. It minimizes cell attachment, protein absorption, and enzyme activation. The surface is noncytotoxic, biologically inert, and nondegradable. Use these products to maintain cells in a suspended, unattached state; prevent stem cells from attachment-mediated differentiation; prevent anchorage-dependent cells from dividing; and reduce binding of attachment and serum proteins to the substrate.


Corning ultra-low attachment surface

IVF Sterile Culture DishTreated for optimal cell attachmentDesigned for in vitro fertilization research. Sterile dish has a 20-mm inner well that holds 3 mL of medium while the outer well holds 10 mL. The inner well isolates oocyte and embryo with easier access. The outer well is designed for humidification and washing. Each dish is certified nonpyrogenic and sterilized by gamma radiation. Note: Not for use in medical applications.


Catalog number Center well dia Dimensions Qty/pk Qty/cs Price/csKH-01936-60 20 mm 60 x 15 mm 20 500

Catalognumber

Wellstyle

Welldiameter

Workingwell volume

Culture area(cm2/well) Lid Qty/

pkQty/cs

Price/cs

96-multi-well cell culture clustersKH-01959-00 6 34.8 mm 5.0 mL 9.5 Yes 5 100 KH-01959-02 1 50 KH-01959-04 12 22.1 mm 3.0 mL 3.8 Yes 5 100 KH-01959-06 1 50 KH-01959-08 24 15.6 mm 2.0 mL 1.9 Yes 1 100 KH-01959-10 1 50 KH-01959-12 48 10.2 mm 1.0 mL 0.8 Yes 1 100

96-well cell culture clustersKH-01959-20

Flat 6.4 mm 0.1 to 0.2 mL 0.32 Yes

5 50 KH-01959-22 1 50 KH-01959-24 10 50 KH-01959-26 5 100 KH-01959-28 Round 6.4 mm 0.1 to 0.2 mL — Yes 1 50

Catalog number Dish style Qty/pk Qty/cs Price/csKH-01835-20 Round dish, 60-mm dia 5 20 KH-01835-22 Round dish, 100-mm dia KH-01835-24 6-well plate KH-01835-26 24-well plate 1 24 KH-01835-28 96-well plate

01936-60




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LILife Science




Lab-Tek II Chamber Slide SystemGlass slides are specially cleaned for optimal cell attachment and growth. Separate the media chamber from the slide using removal tool (included). Biocompatible acrylic adhesive seals the media chamber and slide, preventing well-to-well contamination. Wells feature a hydrophobic border that defines their growth area. Superfrost® writing area allows for easy sample identification.

AccessoriesKH-01930-51 Cover glass for SlideFlask 01838-31. Case of 1000KH-01930-52 Cover glass for 16-well Chamber Slide system 01838-28. Two-ounce pack

Lab-Tek Chambered Cover Glass SystemsSystems consist of a miniature polystyrene media chamber with a #1 borosilicate cover glass as the growth surface. Chambered cover glasses are sealed to the media chamber using a nontoxic silicone gasket. Ultra-thin (0.13 to 0.17 mm) cover glasses permit viewing of cells at higher magnifications using an inverted microscope. Special German-origin borosilicate glass is ideal for growing fastidious cells such as neuronal cells. Media chambers are not removable.

Ideal for in situ analysis—cells can be cultured, fixed, stained, and viewed on the same microscope slideEasily perform microscopic analysis of cell cultures using these miniature chamber systems that have removable media chambers. This eliminates the need for cell transfers from a vessel to a slide. The systems consist of a lid for aseptic manipulation, removable polystyrene (PS) media chamber, and a standard 25 x 75 mm slide that functions as the growth substrate.

Choose from either cell-culture treated Permanox® plastic or glass (soda lime) slides. Both are ideal for analysis of fluorescent stained cell cultures as they do not interfere with fluorescent labels. All Chamber Slide systems are presterilized.

Lab-Tek Chamber Slide SystemsPermanox and PS slides are cell-culture treated; glass slides are precleaned to promote cell adhesion and growth. Media chambers are easily removed by hand leaving the slide for further analysis. Chamber and slide are sealed with a nontoxic silicone gasket which can be removed or left in place for reagent incubation. SideFlask and Flaskette systems are great for closed incubation.

Lab-Tek® Chamber Slide™ Systems

Developed for microscopy of in situ cell culture

01838-00

Optimal surface for excellent attachment and growth of cells

Chambered cover glass media chamber is not removable.

Catalognumber

Numberof wells

Well growtharea(cm2)

Workingvolume

(mL)

Slidematerial

Qty/pk

Price/pk

KH-01838-20 1 9.4 2.5 to 4.5 Glass 16 KH-01838-21 Permanox KH-01838-22 2 4.2 1.2 to 2.0 Glass 16 KH-01838-23 Permanox KH-01838-24 4 1.8 0.5 to 0.9 Glass 16 KH-01838-25 Permanox KH-01838-26 8 0.8 0.2 to 0.4 Glass 16 KH-01838-27 Permanox KH-01838-28 16 0.4 0.1 to 0.2 Glass 16 KH-01838-30 1–Flaskette 10.0 2.5 to 5.0 Glass 16 KH-01838-31 1–SlideFlask 9.0 2.5 to 5.0 PS 5

Catalognumber

Numberof wells

Growtharea(cm2)

Workingvolume

(mL)

Slidematerial

Qty/pk

Price/pk

KH-01838-00 1 8.6 2.0 to 4.5

Glass 16

KH-01838-01 2 4.0 1.0 to 2.0 KH-01838-02 4 1.7 0.5 to 1.0 KH-01838-03 8 0.7 0.2 to 0.5

Catalognumber

Numberof wells

Growtharea(cm2)

Workingvolume

(mL)

Slidematerial

Qty/pk

Price/pk

KH-01838-10 1 9.4 2.5 to 4.5

Glass 16

KH-01838-11 2 4.2 1.2 to 2.0 KH-01838-12 4 1.8 0.5 to 0.9 KH-01838-13 8 0.8 0.2 to 0.4

See our “Microscopes” section on pages 935–962 and our “Histology”section on pages 696–700 for microscopes and microscope accessories.



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Thermanox® CoverslipsCell culture treated on one side for optimal cell growth and attachmentl Flat and easy-to-handle—0.2 mm thicknessl Thermanox® plastic is resistant to solvents commonly used

in electron microscopy.l Cut, section, or peel to desired size without shatteringThermanox® exhibits low autofluorescence—large peak 214 nm; small peak 275 nm. Coverslips are radiation sterilized.


ConversionsNo. cells per mm3 = (No. cells counted per mm2) (dilution) (10)No. cells per mL = (No. cells counted per mm2) (dilution) (10,000)1 mL = 1000 mm3 1 µL = 1 mm3

NuncTM Thermanox® coverslips

Available in three membrane materials and five pore sizes, these permeable supports satisfy your diverse cell culture requirements. A patented suspended design prevents media wicking between the insert and the plate well. It also allows for co-culturing without damaging cells attached to the plate’s growth surface. Openings in the insert wall permit pipet access to the plate well.

Choose either a polycarbonate (PC), polyester, or PTFE collagen-coated membrane. PC and polyester membranes are cell culture treated for optimal cell attachment. PC membranes are compatible with most organic fixatives and stains. Supports come sterile and preloaded in cluster plates or dishes.

Provide an environment for cells that closely resembles their in vivo statel Ideal for a variety

of cell culture applications— chemotaxis, drug transport, tissue remodeling, and invasion studies

Transwell™ Permeable Supports

Transwell Support Selection Guide


Costar® Transwell™ permeable supports

19480-06

*Model 19480-06 is not cell culture treated.†To order Multidish®, see page 1020.

Sterile Microscope SlidesSurface treatment ensures optimal conditions for cell attachment and growthThese sterile microscope slides are cell culture treated—ideal for tissue culture growth and microscope applications. The Permanox™ plastic slide offers minimal fluorescence side background. The polystyrene slide autofluorescences less than 485 nm. Both slides feature a “sand blasted” frosted end for writing.

Specification & Ordering Information

Catalog number Material Dimensions Qty/pk Qty/cs Price/cs

KH-01839-07 Permanox 27 x 75 mm 20 100 KH-01839-08 Polystyrene

018907-07

Catalognumber

For use withMultidish size†

Dimensions(mm)

Qty/cs

Price/cs

KH-01839-00 24 well 13 dia

500

KH-01839-01 6 well 25 dia KH-01839-02 4 well 60 L x 22 W KH-01839-03 8 well 22 L x 10.5 W KH-01839-04 — 30 L x 24 W KH-01839-05 — 15 dia KH-01839-06 — 22 dia

Catalognumber

Clusterplate size

Membrane Qtycs

Price/csMaterial Diameter

(mm)Growth area

(cm2)0.1-µm supports with cluster platesKH-19480-06* 24 PC 6.5 0.3 48 0.4-µm supports with cluster platesKH-19480-00 6

PC

24 4.5 24 KH-19480-03 12 12 1.1 48 KH-19480-07 24 6.5 0.3 48 KH-19480-11 100-mm dia 75 44 12 KH-19480-13 6

Polyester24 4.5 24

KH-19480-15 12 12 1.1 48 KH-19480-17 24 6.5 0.3 48 KH-19480-19 6 Collagen-

treatedPTFE

24 4.5 24 KH-19480-21 12 12 1.1 48 KH-19480-23 24 6.5 0.3 48 5.0-µm supports with cluster platesKH-19480-09 24 PC 6.5 0.3 48 8.0-µm supports with cluster platesKH-19480-02 6 PC 24 4.5 24 KH-19480-10 24 6.5 0.3 48 12.0-µm supports with cluster platesKH-19480-05 12 PC 12 1.1 48

Membrane type Polyester Polycarbonate PTFE/CollagenOptical properties Clear Translucent Clear when wet

Cell visibility, microscopy only Yes No Cell outlines Cell culture treatment Yes Yes* No

Collagen treatment No No YesMembrane thickness (µm) 10 10 50

Pore sizes (µm) 0.4, 3.0 0.1, 0.4, 3.0,5.0, 8.0, 12.0 0.4, 3.0



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LILife Science

19480-28

19480-5019480-51

PP

PS

63600-30

06339-90

34534-30

06754-93

Sterile culture tubes come with

two-position caps.

See our “Cryogenics” section on pages 334–339 for related products.

Netwell™ InsertsIdeal for immunocytochemical staining, explant culture, 3-D tissue culture, and coarse filtration of cell suspensionsl Designed for use as tissue carriers and strainersSterile inserts are polystyrene inserts fitted with polyester mesh bottoms. Choose from two mesh sizes and two diameters. Supplied preloaded in 6- or 12-well Cluster plates.

Netwell Reagent TraysBlack tray provides better visibility for tissue culture sections; white tray is ideal for colorimetric reaction contrast.


Disposable Sterile Culture Tubes Heavy-walled tubes resist breakage. Choose from clear polystyrene (PS) or natural polypropylene (PP). The 12 x 75 mm tubes have a volume of 6 mL; the 17 x 100 mm tubes have a volume of 16 mL. Tubes are sterile; include two-position PE caps.

Sterile Culture TubesThese tubes are manufactured from optically clear polystyrene and supplied with threaded plug seal caps to prevent leakage. Untreated tubes have a hydrophobic surface that significantly reduces the attachment of most cells. Treated tubes have a hydrophilic and ionic surface that allows optimal cell attachment. Sterilized by gamma radiation and certified nonpyrogenic.

External Thread Cryogenic VialsExternal threads and long skirt length reduce risk of contamination l Self-standing vials are constructed of polypropylene with silicone gaskets l Vials withstand temperatures from –196 to 120°C White marking area aids in sample identification. Graduated on the side. Order colored cap inserts at right.

Colored Cap Inserts for vials

Color-code your cryogenic vials (at left) with these cap inserts for quick identification.

Catalog number Description Color Qty/cs Price/csKH-19480-50 Netwell reagent tray Black 200 KH-19480-51 White 200

Catalognumber Diameter Mesh size Inserts/

plateInserts/

csPrice/

csKH-19480-25 15 mm 74 µm 12 48 KH-19480-26 500 µm KH-19480-27 24 mm 74 µm 6 48 KH-19480-28 500 µm

Catalognumber

Size(mm) Material Color Qty/

pkPrice/

pkKH-06339-90 12 x 75 PP Natural 1000 KH-06339-95 PS Clear 1000 KH-63600-20 17 x 100 PP Natural 500 KH-63600-30 PS Clear 500

Catalog number Surface Capacity (mL) Size (mm) Qty/pk Qty/cs Price/csKH-34534-30 Untreated 15 16 x 125 25 500 KH-34534-32 Treated 50

Catalognumber

Vialsize

DimensionsH x OD (mm)

Qty/pk

Price/pk

EK-06754-93 1.0 mL 39 x 1225

EK-06754-95 1.2 mL 38 x 13.5 EK-06754-94 1.5 mL 48 x 12 EK-06754-96 2.0 mL 48 x 13.5 25 EK-06754-97 5.0 mL 89.4 x 13.5 10

Catalog number Color Qty/pk Price/pkEK-06755-90 White

100

EK-06755-92 Yellow EK-06755-94 Blue EK-06755-96 Green EK-06755-98 Red



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Accurately count white and red blood cells, platelets, tissue culture cells, bacteria, and sperml Smooth capillary action evenly

distributes cellsl H-shaped moat forms two counting

areas with enhanced Neubauer rulingsl V-slash at loading side provides precise

fluid loadingWhen viewed through a microscope, hemacytometers display white Neubauer markings against a dark background. Choose from Bright-Line® hemacytometers with a Pyrex® surface or Hy-lite hemacytometers with a milled glass surface. Bright-Line phase contrast hemacytometer is thinner—ideal for use with phase contrast microscopes. Glass coverslips cover moat region 0.1 mm above ruled surface, limiting volume to 0.1 mm3.

36219-00

Hemacytometers

KH-36219-50 Replacement cover slip



Economical HemacytometerSpecially designed for counting erythrocytes and leukocytesl Two special counting chamber glass coverslipsl Light transmission between 29 and 32%This hemacytometer features high light transmission and brightly illuminated line. Double rulings are visible through a wide range of illumination. Neubauer rulings are 0.1 mm below cover glass. The H-shaped moat offers two counting areas. Fused rhodium on glass provides distinct lines. Two glass coverslips are included.

Disposable HemacytometersPrecise fixed-depth counting chamber with superior accuracy and reproducibilityl Single use—reduces contamination and exposure

to infectious samplesl Light and unbreakablel No coverslips required These disposable hemacytometers are made of durable plastic for manual cell counting. They offer significant advantages over conventional glass models. Disposable, single-use design reduces the risk of exposure to potential infectious materials. Increase productivity by eliminating tedious washing—simply use and toss. Hemacytometers feature two identical 100-µm height chambers with two ports for sample injection. Applications include manual cell counting, cell viability tests, spore counting, and more.

Specification & Ordering InformationDimensions: 25 mm W x 75 mm H x 1.6 mm D

Catalog number Grid type Depth Qty/bx Price/bxKH-79003-00 Neubauer Improved 100 µm

50

KH-79003-02 Fuchs Rosenthal 200 µm KH-79003-04 Semen test 10 µm

Catalog number Description PriceKH-79001-00 Economical hemacytometer

Cat. no. Description Surface Coverslips PriceKH-36219-00 Bright-Line Pyrex Two 0.4 mm KH-36219-05 Hy-lite Milled glass KH-36219-10 Bright-line (phase contrast) Pyrex —


04468-5404468-50 04468-52

Tissue Grinder SystemsEliminate risks of contact with samplel Fully assembled and gamma sterilizedl Narrow pestle eliminates sample overflowThese unique tissue grinder systems are used wherever tissue homogenization is required. The patented closed system has the ability to grind the specimen in a sealed container. The molded-in abrasive surface does not leave any sediment to obstruct the sample during examination. Model 04468-52 features a free-standing design for easier handling.

What’s included: cap and sample identification label.

Catalog number Description Qty/cs Price/csKH-04468-50 15 mL, conical

10

KH-04468-52 35 mL, free standing KH-04468-54 50 mL, conical

ConversionsNo. cells per mm3 = (No. cells counted per mm2) (dilution) (10)No. cells per mL = (No. cells counted per mm2) (dilution) (10,000)1 mL = 1000 mm3 1 µL = 1 mm3

See our “Homogenizers” section on pages 701–710 for related products.



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LILife Science


aspirette™ Vacuum TweezersHandling small, delicate objects just got easier

Capacity: 21/2 oz (70 g)Battery life: 1000 cycles

Dimensions: 23/4" W x 41/2" H x 1"D

AccessoriesKH-61202-50 Air filters prevent dust particles from entering the vacuum pump system. Pack of threeKH-61202-51 Suction nozzle kit includes one each of 3.2-mm, 6-mm, and 10-mm diameter nozzles

61202-00

Pick up, move, and position small, delicate, and hard to hold objects—up to 21⁄2 oz. Ergonomic, lightweight, easy-to-use design—one button operation allows safe, fatigue-free operation. Suction nozzles are autoclavable to accommodate asceptic technique. Interchangeable suction nozzle sizes hold objects gently and securely. Ideal for small scale cell culture applications using slides or coverslips. Anti-static model is ideal for use with small electronic parts and micro chips.

What’s included: suction nozzle kit and battery charger/AC adapter.

Catalog number Power Priceaspirette vacuum tweezers

KH-61202-00 120 VAC or rechargeable NiCd battery KH-61202-05 220 VAC or rechargeable NiCd battery KH-61202-07 240 VAC or rechargeable NiCd battery

aspirette anti-static vacuum tweezersKH-61202-04 120 VAC or rechargeable NiCd battery KH-61202-06 220 VAC or rechargeable NiCd battery KH-61202-08 240 VAC or rechargeable NiCd battery


This polycarbonate filling bell allows aseptic transfer of liquids and cell culture media—ideal for microbiology and cell culture applications. It is nontoxic, durable, and costs less than glass bells. Polypropylene adapter for 1/4" to 1/2" ID tubing is included. Filling tube measures 21/2"L x 3/8"OD. Autoclavable.

Filling Bell

06239-20

Catalog number Dimensions PriceKH-06239-20 41/2"H x 2 "D

Sterile PETG Media BottlesDurable PETG (Polyethylene Terephthalate G Copolymer) bottles with HDPE closures are radiation-sterilized and nonpyrogenic—eliminates washing, depyroenation, and autoclaving steps. Reduced permeability to CO2/O2 allows long-term media storage. Tamper-resistant band around closure and neck of each bottle ensures sterility. Bottles come in shrink-wrapped trays. Nalgene sterile PETG

media bottles

Specifications & Ordering InformationCatalognumber Capacity Closure size Qty/

csPrice/

csKH-06254-00 30 mL 20 mm 96 KH-06254-10 60 mL 24 mm 96 KH-06254-20 125 mL 38-430 mm 48 KH-06254-30 250 mL 38-430 mm 48 KH-06254-40 500 mL 38-430 mm 24 KH-06254-50 1000 mL 38-430 mm 24 KH-06254-60 2000 mL 53B mm 12

Top Works™ Closures for Bottles and Carboysl The ideal aseptic fluid transfer systemFlexible Top Works closures are available in solid, 2-port and 3-port versions. Closures are polypropylene (PP). Platinum-cured silicone tubing is fused to a silicone stopper insert that forms a one-piece closure system. Autoclavable and leakproof.


13070-00

Catalognumber

Closuresize (mm)

No. of insert ports

ID sizes

Recommendedfor Price

EK-13070-00 38-430 None (solid) — Bottles/carboys EK-13070-02 38-430 Three 1/4", 1/8", 1/8" EK-13070-04 53B None (solid) — Bottles/jerricans EK-13070-06 53B Two 1/4", 1/2" EK-13070-08 53B Three 1/8 ", 1/4", 1/4" Bottles/jerricans EK-13070-10 83B None (solid) — Carboys EK-13070-12 83B Two 1/4", 1/2" Carboys EK-13070-14 83B Three 3/8", 1/4", 1/4"

See our “Bottle” section on pages 144–166 for related products.



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LI Life Science

Sterile Disposable Flasksl Reduce cross-contamination suspension in cell cultures, media preparation, or storageSterile, disposable Erlenmeyer flasks offered with either a plain or baffled base. Plain bottom is ideal for containing, mixing and storage of solutions; baffled bottom is designed for use on shaker tables. Flasks are graduated for quick volume assessment. Clear polyethylene terephthatlate co-polyester plastic (PETG) with leakproof, polyethylene screw closures.

06112-62

06112-54

Catalognumber

Capacity(mL)

Bottomshape

Qty/pk

Price/pk

EK-06112-60 125

Flat

24 EK-06112-62 250 12 EK-06112-64 500 12 EK-06112-66 1000 6 EK-06112-68 2000 4 EK-06113-50 125

Baffled

24 EK-06113-52 250 12 EK-06113-54 500 12 EK-06113-56 1000 6 EK-06113-58 2000 4

13050-30

13050-32A B

EC

DKH-13050-32 Hand controller set includes hand operator; (E), adapters A, B, and C; rubber adapter for pasteur pipettes; safety stand; Swiss army knife; and 1.8-m silicone tubing KH-13050-34 Hand operator (E), includes silicone grease and rubber adapter for pasteur pipettes KH-13050-36 Eight-channel plastic aspirator (D) for disposable tips with ejection device

Vacusafe Waste Disposal SystemSafe, compact, and flexible aspiration device for your biological wasteThe Vacusafe comfort is designed to collect critical liquids through vacuum and variety of hand operator. All parts, except the housing containing the pump, are fully autoclavable. The 4-liter polypropylene bottle can be swapped out and autoclaved with minimum effort. Replace with new bottle in just seconds. A hydrophobic filter prevents liquids getting into the pump and pump housing in the event of an overflow. The vacuum can be infinitely controlled to meet your precise needs.

The hand operator 13050-34 is designed to fit any vacuum device currently available on the market. Set vacuum control by manual adjustment of the aperture with the control button. Use with pasteur pipetts or any adapter listed below.

What’s included: Vacusafe comfort system, 4-L PP bottle, one lid without tube couplings, one lid with tube couplings, and tube set with filter.

Catalog number Description PriceKH-13050-30 Vacusafe comfort

Air Admiral® Aspiration SystemsCompact, lightweight, and portable aspiration systeml Dial vacuum gauge and regulator let you monitor and adjust vacuum levell Automatic flow shutoff when fullSystem features an autoclavable collection receiver made of polypropylene, a hydrophobic in-line filter to prevent introduction of the aspirate into the pump, and automatic shutoff to prevent overflow of the aspirate to protect you and the pump from exposure to the media. See “Vacuum Pumps” for complete details.

What’s included: 1.2-L collection vessel, two hydrophobic filters, 6-ft PVC tubing, cord, and plug.

79202-20

Catalog number

Free-air capacitycfm (L/min)

Max vacuumin of Hg

Max pressure

Max temperature

Motor Noise rating Port size Power

(VAC) Amps Dimensions(L x W x H) Price

Type hpKH-79202-20 0.39 (11) 21.3" 33 psi 104°F (40°C) Shaded pole 1⁄4 57 dB(A) 3⁄16" hose barb 120 1.6 11" x 81⁄4" x 10" KH-79202-25 230 0.8 KH-79202-30 1.2 (34) 27.2" None 104°F (40°C) Single pole 1⁄4 62 dB(A) 1⁄4" hose barb 120 4.6 14 3⁄4" x 81⁄4" x 10" KH-79202-35 230 1.5

Specifications & Ordering InformationWetted parts Models 79202-20 and -25: glass-filled polyester piston cup retainer and

head, Viton® cylinder sleeve, Viton valves, PTFE piston cup, silicone tubing, Delrin® fittings

Models 79202-30 and -35: aluminum (treated for corrosion resistance), stainless steel, PTFE, Viton valves, PTFE piston cup, silicone tubing, Delrin fittings

Duty cycle: continous



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MantaRay™ Single-Use Cell Culture FlaskPresterilized, disposable flask minimizes contaminationThe single-use MantaRay™ flask features a unique impeller for gentle stirring of suspension and microcarrier solutions. The proprietary stirrer technology is designed to minimize damage to fragile cells, such as mammalian cells. It is manufactured from flexible plastic that reduces the chance of broken shards, should it be dropped. This space-saving flask also provides the same head space as spinner flasks of a similar volume without the height. Application includes mammalian, plant and insect cell cultures; it can also culture cells on micro-carrier beads. The MantaRay also allows media and gases to be added or changed during the experiment. Sterile magnet stir bar is included.


Mini Suspension Culture SystemsPerfect for small scale operations, or quick tests of your fermentation processThese spin flasks are designed for microcarrier and suspension cultures, such as insect cells, hybridomas, and adapted cell lines.

Use the 45-mm sidearms as air vents, inoculation ports, or media inlet/outlet ports, or use sidearms to insert probes for pH, dissolved oxygen, or CO2 measurement and control. Each spin flask features a bottom dimple to prevent cell crushing and improve circulation. Flasks are made of borosilicate glass, and include a Teflon® and glass impeller assembly. Sidearms have Teflon-faced silicone liners. Top (center) cap has two liners: one silicone and one Teflon (to be used together). Entire spin flask unit is autoclavable. Additional sizes are available; please call our Application Specialists for details.


Excellent for small-scale applications using a magnetic stirrerl Polycarbonate 1-liter vessel is lightweight and impact resistantl Teflon® TFE and PP stirring assembly and Teflon® TFE stir bar create efficient

top-to-bottom mixing at low speeds and low shearl Impeller height is adjustable to maximize mixing at various liquid levelsTop closure is 63 mm; two closures on shoulder access ports are 38-430 mm.

What’s included: two magnetic stir bars; one for bacteria and another for mammalian cells. Vessel and components are autoclavable.

Magnetic Stirring Culture Vessel


12-Liter Culture Vessell Lightweight and impact resistant—polycarbonate vessels are

an excellent alternative to glassl Noncytotoxic, food-grade resin meets USP Class VI requirementsl Ideal for small-to large-scale pharmaceutical bioprocessingl Graduated in 0.5-L increments from three to 12 litersl 100-mm polypropylene (PP) top closure; four 38-430 PP side closures


Catalog number Dimensions PriceKH-50306-00 101/2"H x 53/8" OD

Catalog number Description Dimensions (H x OD) PriceKH-50301-50 Twelve-liter culture vessel 167/8" x 113/8"

Catalog number Working volume (mL) Qty/cs Price/csKH-29300-20 400 to 1000 1 KH-29300-22 4

Catalog number Capacity Dimensions (H x OD) PriceKH-29300-00 500 mL 71/2" x 421/64" (190 mm x 110 mm) KH-29300-02 1000 mL 927/32" x 51/8" (250 mm x 130 mm) KH-29300-04 3000 mL 137/16" x 7" (341 mm x 178 mm)

Magnetic stirring culture vessel 50306-00

50301-50

29300-00

29300-20



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LI Life Science

Disposable Spinner FlasksReady-to-use vessel system eliminates assembly timel Unique paddle and integrated magnet These vessels are made of USP XXXIII class VI polystyrene, but are comparable to conventional glass spinner flasks for growth of suspension cell lines and any attachment-dependent cultures using microcarrier beads. Molded from virgin polystyrene and gamma-irradiated, each single-use flask system ensures a clean sterile unit. The paddle size and height is adjustable for each vessel size. The integrated magnet provides smooth, even rotation at typical stirrer speeds. Heat buildup in the vessel is reduced by means of a specially designed flange that raises the vessel off the stirrer surface.

01835-80


2D Microhex™ MicrocarrierMicroscopic polystyrene hexagons for adherent cell cultureSterile microcarrier features a certified surface treatment to ensure excellent conditions for cell attachment and growth. It is also solid, nonswelling, nonporous, and nonabsorbing. Reagents and gel particles cannot be trapped in the carrier. The shape and low specific gravity of the Microhex enables suspension at low stirring speeds—facilitating the initial cell attachment and resulting in a uniform coverage of all carriers.

Catalog number Culture area Amount Qty/cs Price/csKH-01928-80 1500 cm2 2 g/bottle 10 bottles

Catalog number Capacity Center neck dia Side arm dia Qty/cs Price/csKH-01835-80 125 mL 70 mm 25 mm 12 KH-01835-82 500 mL 100 mm 45 mm

Cultivation area: 760 cm2 gDensity: 1.05 g/cm3

Hexagon side length: 125µHexagon thickness: 25µ


Handheld Barcode ScannerReads Code 39, Code 128, Interleaved 2 of 5, and morel Durable and low costl Keep track of your samplesScanner has a “plug and play” feature for use with a USB slot—great for uploading data right to your computer. It automatically switches between Code 39, Code 128, Interleaved 2 of 5, and several other barcodes. Scanner must be in contact with the barcode to read. Uses English UK language.


Catalog number Description Language PriceKH-01928-82 Scanner for USB English UK

BarcodingA barcode is a piece of automatic identification technology that stores information. Barcodes are “machine-readable codes” which can be used to reduce errors, process many samples, track products, and many more applications. We offer three different code types:

Code 39This is an alphanumerical code that contains both numbers and characters/letters. Code 39 has fewer characters/letters but is printed in the same space. Code 39 characters have five bars and four spaces. It is widely supported, flexible, and offers good data security.

Code 128This is also an alphanumerical code that contains both numbers and characters/letters. Code 128 characters are denser than Code 39 but are printed in the same sign space. They comprise of three bars and three spaces with three different subsets A, B, or C. We recommend Code 128 because of its compressed design, wide support, flexibility, and data security.

Interleaved 2 of 5This code is numerical (no letters) and self-checking to improve data security. Each Interleaved 2 of 5 character encodes two digits (one in the bars and one in the spaces) giving it a higher density. Interleaved 2 of 5 always requires an equal number of characters (including check digit) to be printed. Due to the design of Interleaved 2 of 5, there is a risk of truncation of the sequence when scanned, which is why we recommend a check digit and that a fixed number of digits mode is chosen in the reading software if possible.



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LILife Science

ProCulture® Spinner FlasksGet better cell production and viability than conventional flasksThese spinner flasks offer unsurpassed performance and convenience. Flasks come equipped with a unique extended paddle design to create a constant flow of fluid from the bottom to the top of the flask portions of flask—providing homogenous circulation of cells and medium while ensuring oxygenation and circulation of nutrients. Spinner flasks can be operated by using magnetic stirrers.

ProCulture spinner flasks are equipped with large 32- or 45-mm sidearm openings that easily accept 50-mL pipettes. Flasks with 100-, 120-, and 140- mm center neck are also available in 6-, 8-, 15-, and 36-L sizes; call our Application Specialists for details.

What’s included: borosilicate spinner flask base unit includes two angled sidearms and closed caps, either a 70-, 100-, or 120- mm standard center neck screw cap assembly, and a polysulfone shaft with a vortex impeller paddle assembly. Flasks with 140-mm center neck come with a Shur-Loc cap assembly. Vented.

Specification & Ordering InformationCatalog number Capacity Center neck dia Sidearm dia Price/ea

KH-01837-00 125 mL 70 mm 32 KH-01837-02 250 mL 70 mm 32 KH-01837-04 500 mL 100 mm 45 KH-01837-06 1 L 100 mm 45 KH-01837-08 3 L 100 mm

45

KH-01837-10 3 L 120 mm KH-01837-14 3 L 140 mm KH-01837-12 6 L 120 mm KH-01837-16 6 L 140 mm

Optional angled sidearm fittings (for gas and media handling) let you set up a complete bioreactor at a fraction of the cost of an off-the-shelf unit. Dual-angled sidearm fittings can also be used to sparge the cell culture medium with gases to enhance cell production. Combination-angled sidearm fittings let you transfer media in or out of flasks aseptically.

All fittings are comprised of a PET insert with Viton® O-ring and polypropylene sealing cap. One or two 316 stainless steel tubes, which extend to the bottom of the flask, are held in place by Noryl® nuts with integrated ferrules. For combination style fittings, the other tube is a shorter 6" length. The fittings are completely autoclavable. Fittings with 1/8” OD are also available for all sizes; call our Application Specialists for more options.

Optional vented caps for sidearm fittings have a 0.2 µm pore, nonwettable (hydrophobic) or PEFE membrane sealed directly to the cap to allow gas exchange while minimizing the risk of contamination. Sterilize by gamma irradiation.

Catalog number Style Flask size Tubing OD Price/eaKH-01837-40 Dual 1 L 1/4" KH-01837-42 3 L KH-01837-44 Combination 1 L 1/4" KH-01837-46 3 L

Impeller assembly for magnetically-driven bioreactor with probes. Use optional stainless steel impeller with modified impeller blade with probes to create a small bioreactor. Impeller assembly is available for 3- to 36-L flasks; call us for more details.

Direct drive motors. For high-density or large-volume applications, a direct drive motor unit is the perfect solution. High-torque motor with direct drive coupling makes the transition from magnetic slow speed stirrers easy and efficient. Motors are not autoclavable.

Catalog number Diameter Pore size Filter media Qty/cs Price/csKH-01837-52 45 mm 0.22 µm PTFE 10 KH-01837-54 33 mm 0.2 µm Hydrophobic 5

Catalog number Fits flask size Price/eaKH-01837-50 3 L

Catalog number Speed Power Price/eaVAC HzKH-01837-20 350 rpm 120 60 KH-01837-22 230 50

01837-00

01837-10

01837-14



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LI Life Science

Wheaton Benchtop Roller Culture ApparatusCompact roller system for small bottlesExtremely compact unit needs little benchtop space. Conventional-type roller culture equipment handles minimum of two bottles; 108 to 121 mm in diameter and up to 290 mm long. Two outside rollers can be moved inward to accommodate bottles as small as 75 mm in diameter. Maximum temperature for use is 40°C. Supplied without bottles, order deck kit separately below.

The deck kit 29300-12 attaches quickly and easily to bench model and holds an additional 2 roller bottles per kit. A maximum of two deck kits can be added.

What’s included: Each deck kit consists of assembled roller deck, four support posts, and drive belt.

Low-Profile Bottle RollerLightweight and portable—easy to transport from lab to labl Use from 0 to 60°C–operates in CO2 and high humidity incubatorsl Accommodates tubes from 10 mL up to 850 cm2

Use this low-profile bottle roller for growing bacteria and cell cultures. The low-profile shape saves benchtop and incubator shelf space. The thin, flat power cord reaches external outlets without damaging incubator gaskets. Adjust roller speed from 1 to 30 rpm. The unit comes with 6 rollers to accommodate tubes from 10 mL to 50 mL or up to three 850 cm2 growth area culture bottles with a total of 11⁄2 liters of liquid. The low-profile roller measures 15"L x 141⁄4"W x 23⁄8"H.

What’s included: low-profile bottle roller, six rollers, and 6-ft power cord.


KH-36202-50 Additional bottle roller

Glass Roller Bottles l Avaliable in 670, 1330, and 1585 cm2

These reusable bottles feature a wide mouth for simple cell harvesting and cleaning. Manufactured from borosilicate glass for optical clarity and mechanical strength; can withstand repeated wet or dry sterilization.

What’s included: GPI 51-400 threaded, 51-mm phenolic screw cap with rubber liner.


Catalog number Growth area Closure type Dimensions Qty/cs Price/csKH-01835-90 670 cm2

51-mm screw cap110 x 270 mm (41/3" x 105/8" )

2

KH-01835-92 1330 cm2 110 x 475 mm (41/3" x 1811/16") KH-01835-94 1585 cm2 110 x 570 mm (41/3" x 227/16")

Specification & Ordering InformationBottle speed: 0.1 to 3.8 rpm based on 110 mm bottle Dimensions: 121/4"W x 123/4"H x 71/8"D

Catalog number Description Power PriceKH-29300-10

Single deck bottle roller120 VAC North America

KH-29300-14 230 VAC Europe KH-29300-16 230 VAC UK KH-29300-12 Deck kit —

Catalog number Power (50/60 Hz) PriceKH-36202-02 115 VAC KH-36202-04 220 VAC

KH-01835-96 Replacement screw cap

29300-10

36202-02

01835-92 01835-90



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LILife Science

01836-0001836-01 01836-06

01836-02

Nunc PETG roller bottles

Specifications & Ordering InformationCatalog number Volume Price

EK-29206-40 1 L EK-29206-44 3 L EK-29206-48 7 L EK-29206-52 15 L

Cap TypesPlug seal cap features a one-piece, linerless construction for a liquid-tight seal.Easy grip cap is designed with large knurls and smooth edges for easy opening. Caps twist a half turn for tightening or removal.Easy grip vent cap has the same features as the easy grip cap plus a 0.2-µm membrane sealed into the cap for sterile gas exchange.

Roller BottlesSeamless, one-piece construction reduces risk of breakagel Cell culture treated for optimal cell attachmentl Individually printed with lot numbers for easy identification and traceabilityl New CellBIND surface for improved cell attachmentOptically-clear polystyrene roller bottles are sterilized by gamma radiation and certified nonpyrogenic. All bottles, except for model 01836-00, have black printed graduations. Caps have smooth edges and large knurls for easy opening—just twist a half turn. Roller bottles with vent caps are ideal for applications requiring continuous gas exchange.

Models 01836-05 and -06 feature a ribbed design that doubles the growth area without increasing bottle size. Two window areas provide microscopic viewing as well as pouring surfaces. Models 01835-40 and –42 feature CellBIND™ surface which enhances cell attachment under difficult conditions. Roller bottles with this special surface reduce premature cell detachment from confluent cultures, resulting in higher cell yields.



InVitro PETG Roller BottlesSurface areas range from 1050 cm2 to 4200 cm2

l Expanded surface bottles have increased surface-to-volume ratiosl Molded of durable PETG—excellent substrate for adherent cell culturesl Ergonomic closure reduces wrist strain and increases productivityChoose standard smooth surface or patented expanded surface (XPS). Polyethylene terephthalate copolyester (PETG) offers superior impact resistance over glass or polystyrene while providing an excellent gas and vapor barrier. Quick-action ergonomic closure and easy-to-read graduations make these bottles easy to use. Model 01838-70 features vented closures with 0.22 µm hydrophobic membrane—ideal for use with cell lines that require additional CO2. Gamma-radiation sterilized. Lot number on each bottle maximizes traceability.

Temperature range: –40 to 60°C

BioBundle™ Fermentation SystemsEconomical, complete and ready to assemblel Affordable—save up to 30% with BioBundle systeml Complete systems include everything you need for a wide range of applicationsl Easy—packaged for fast, trouble-free assembly, setup requires no special skills or tools BioBundle™ culture systems are the fastest, easiest, and most economical way to get your projects up and running. Complete and ready to assemble—every BioBundle system is made of the finest materials available and built to GMP standards. All materials in contact with the bioreactor vessel are constructed of either 316L stainless steel, EPDM, silicone rubber, PTFE, or borosilicate glass.

BioBundle bioreactors are available for mammalian cell culture or microbial processes, including bacteria, yeast, insect, fungi, and plant cells. The P100 motor includes a 6:1 gear reduction to provide higher torque capability. All units are 110 VAC; for 220 VAC models, go to page 440 or call our Application Specialists for availability.

Catalog number

Surface treatment Growth area Working

volume Cap type Qty/pk Qty/cs Price/cs

KH-01836-00Standard

490 cm2 100 to 150 mL Plug seal 2 40 KH-01836-01

850 cm2 170 to 255 mLEasy grip 2 36

KH-01836-02 Easy grip vent cap KH-01835-40 CellBIND Easy grip 2 36 KH-01836-05 Standard 1700 cm2 340 to 510 mL

Easy grip 2 36 KH-01836-06 Easy grip vent cap 1 20 KH-01835-42 CellBIND Easy grip 1 20

Catalog number Growth area Closure type Working volume Qty/pk Qty/cs Price/csKH-01838-60 1050 cm2 Standard 100 to 500 mL 5 20 KH-01838-70 1050 cm2 Vented 5 20 KH-01838-62 1800 cm2

Standard 200 to 1000 mL 22 20 KH-01838-64 1700 cm2 200 to 600 mL 20 22 KH-01838-66 2100 cm2

Standard 200 to 600 mL 5 20 KH-01838-68 4200 cm2 400 to 10000 mL 22 22



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LI Life Science

Bioprocess TanksSpecially integrated design satisfies bioprocessing requirementsClosed-Dome HDPE Tanks are a low-cost alternative to SS tanks—ideal for mixing, storing and dispensing reagents. Smooth, seamless design ensures efficient mixing. The 61/4" threaded fill port accommodates an HDPE closure and EPDM gasket. Solid closure provides a leakproof seal that reduces evaporation and prevents contamination. Two-inch bung with cap permits access for tubing or piping. Translucent walls allow easy viewing of liquid levels.

Graduations on 115- and 210-liter tanks are every 10 liters (2.5 gallons); graduations on 380-liter tanks are every 50 liters (10 gallons). Optional overhead Biotech mixer available at www.coleparmer.com; requires mixer support assembly 50301-70 sold separately below.

Closed-Dome PP Tanks are great for preparing media components and growing cultures. Large 6" threaded opening makes filling and dispensing easy. PP closure and gasket material meet specifications determined safe by the Federal Food, Drug and Cosmetic Act.

The 75-liter tank has molded-in body grips for safe and convenient handling. Graduations on 75-, 115-, and 210-liter tanks are every 10 liters (2.5 gallons); graduations on 380-liter tanks are every 50 liters (10 gallons). Optional overhead Biotech mixer available at www.coleparmer.com; requires mixer support assembly 50301-70 sold separately below.

KH-50301-70 Closure with mixer support assembly, PP. Required to attach Biotech mixer and lower assembly to the closed-dome tanks listed above. Includes 6" PP screw closure with 2" sanitary ferrule welded in the center, 2" silicone gasket, and PVDF true union fitting. Can be connected to other 2" sanitary fittings. AutoclavableKH-50301-95 Stainless steel dolly helps transport tanks up to 115 liters. SS is noncorrosive and resistant to acids and bases. Autoclavable. Measures 201⁄2" ID x 61⁄2"H

Siphon Tubes provide easy fluid transfer when using a peristaltic or vacuum pump. PP is noncytotoxic. To use siphon tubes, fittings must be installed on tank.

Closure with mixer support assembly 50301-70.

Closed-dome bio tanks 06318-70

Sterile Bio-BagsUse these sterile containment bags for storage, mixing, collection, and transfer of buffers, reagents, and other liquidsReduce costs associated with expensive and tedious cleaning validation with these sterile, single-use, biopharmaceutical containment bags. Disposable bags are manufactured from USP Class VI grade films and have an FDA Drug Master File number. Each shipment comes with certificates of compliance and irradiation, and with lot-traceable numbers.

All bags are equipped with three ports; inlet and outlet, and a “swabbable” needleless port used for sampling. The bio-bags are made of LLDPE/EVA Coex and the buffer prep/formulation bags are made of multi-ply LDPE.

13300-20Sterile, disposable

bags save time and money!


Fittings for Bio-Bags with Quick-Disconnect Fittings

KH-31305-20 Male quick-disconnect fitting; 1⁄4" tubing ID, polycarbonate. Pack of tenKH-06360-94 Female quick-disconnect fitting; 1⁄4" tubing ID, Delrin®. Pack of ten

Siphon tube 50301-91

Stainless steel dolly 50301-95


Catalog number Capacity Sanitary

mountTube dimensions

PriceID OD Length

KH-50301-91 75 L (20 gal.)

51 mm (2") 16 mm (5⁄8") 25 mm (0.985")

92 cm (361⁄3") KH-50301-92 115 L (30 gal.) 103 cm (401⁄2") KH-50301-93 208 L (55 gal.) 115 cm (451⁄3") KH-50301-94 378 L (100 gal.) 135 cm (53")

Size Catalog number Qty Price/

eaCatalog number

Qty/ cs

Price/ cs

Bio-bags with 1⁄4" tubing ID barbed connections5 L KH-13300-00 KH-13300-50 10 L KH-13300-05 1 KH-13300-55 10 20 L KH-13300-10 KH-13300-60

Bio-bags with CPC female and male quick-disconnect fittings5 L KH-13300-15 KH-13300-65 10 L KH-13300-20 1 KH-13300-70 10 20 L KH-13300-25 KH-13300-75

Buffer prep/formulation bags for single-use containment50 L KH-13300-30 KH-13300-80 100 L KH-13300-35 1 KH-13300-85 10 200 L KH-13300-40 KH-13300-90

Catalog number Capacity Dimensions Wall thickness PriceClosed-dome HDPE tanks

KH-06318-70 115 L (30 gallons) 385⁄8"H x 181⁄2" OD 0.187" KH-06318-75 210 L (55 gallons) 431⁄4"H x 22" OD 0.250" KH-06318-90 380 L (100 gallons) 52"H x 28" OD 0.250"

Closed-dome PP tanksKH-50301-53 75 L (20 gallons) 32"H x 161⁄2" OD 0.250" KH-50301-52 115 L (30 gallons) 385⁄8"H x 181⁄2" OD 0.250" KH-50301-54 210 L (55 gallons) 431⁄4"H x 22" OD 0.250" KH-50301-56 380 L (100 gallons) 52"H x 28" OD 0.312"



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LILife Science

Go to page 1851 for custom assemblies for your bioprocessing system.

1851

2 Conical DrumsThe Mixtainer system utilizes a polyethylene drum with a special enlarged opening at the bottom to accommodate the stir-bar dish. Reusable.

3 Maxi StirrersThe Mixtainer and drum must integrate with the Maxi Stirrer magnetic stirring plate. Reusable.

1 Single-Use Mixtainer BioProcess ContainersSpecification & Ordering Information

Material: animal-derived component- free (ADCF) HyQCX5-14

Port 1: 1/4" (6.4 mm) male luer lock, 4 ft 8" (121.9 cm) tube length

Port 2: 1/4" (6.4 mm) female luer lock, 4 ft 8" (121.9 cm) tube length

Port 3: 3/8" (9.5 mm) quick connect insert, 4 ft 8" (121.9 cm) tube length

Port 4: 3/8" (9.5 mm) quick connect insert, 4 ft 8" (121.9 cm) tube length.

l Medical-grade, animal-derived component-free film

l Completely disposable, minimize the risk of cross contamination

The Mixtainer™ system allows for closed loop liquid-liquid and liquid-powder mixing of cell culture media, process fluids, and reagents and cell suspensions. This unique integrated single-use mixing system consists of a single-use Mixtainer BioProcess Container which contains a magnetic stir-bar in a concave carrier, a supporting conical drum, the Maxi Stirrer magnetic stirring plate, and a specially designed dolly for concentric alignment of the system components. Mixtainers systems are available in 50, 100, and 200 L sizes.

1 Single-Use Mixtainer BioProcess Containers (BPC)The Mixtainer BPC contains a magnetic stir-bar located in a plastic dish welded in the bottom of the BPC. The dish contains a locking ring to ensure the containment of the stir-bar during shipping, handling, and operation. The BPC is constructed of HyQ® CX 5-14, a 14-mil thick medical-grade film which is animal derived component free (ADCF), and is the biopharm’s industry choice due to cleanliness, outstanding tensile properties, low gas permeability, and low profile of leachable compounds.

Individual Mixtainer BPCs are gamma irradiated, single polybagged, and sealed in a second polybag. All end fittings are capped or plugged and protected with a resealable polybag. All tubing lines are C-FLEX® and provided with clamps.

Typical applications include sterile mixing of cell culture media with sera and reagents, sterile mixing of buffer solutions, sterile mixing of in-process fluids, sterile mixing of microcarrier solutions, storage of process liquids, and resuspending of process fluids.

4 DollyThe dolly is designed to interface between the drum and the Maxi Stirrer to create the vertical and concentric alignment necessary for operation. Reusable.

HyClone® Mixtainer™ Bioprocessing SystemIntegrated single-use sterile system for mixing and maintaining homogenous aqueous solutions

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Catalognumber Description Capacity Dimensions

(D x H) Price

KH-06341-20 1 port, clamps, bottom dispense

50 L 233⁄8" x 211⁄2" KH-06341-22 100 L 233⁄8" x 281⁄2" KH-06341-24 200 L 233⁄8" x 431⁄2"

Catalog number Power PriceKH-06341-30 120 VAC, 50/60 Hz, 1.4 A KH-06341-32 220-240 VAC, 50/60 Hz, 0.7 A with European cord

Catalognumber Description Dimensions

(D x H) Price

KH-06341-34 Dolly, holds Maxi stirrer and conical drum 243⁄8 x 71⁄8"

Catalog number Description Capacity PriceKH-06341-00

4 ports, top dispense50 L

KH-06341-02 100 L KH-06341-04 200 L

Catalognumber

Capacity(mL)

Tubematerial

Max RCF(x g)

Cap style (cap included) Sterile Packaging Qty/

pkPrice/

pkEK-06344-20 15 PS 1800 Screw cap/rim seal No 1 bag of 500 500 EK-06344-21 15 PS 1800 Screw cap/rim seal Yes 10 trays of 50 500 EK-06344-22 15 PPCO 6400 Screw cap/rim seal No 1 bag of 500 500 EK-06344-24 15 PPCO 6400 Screw cap/rim seal Yes 20 bags of 25 500 EK-06344-27 50 PPCO 10,000 Flat top/rim seal No 1 bag of 500 500 EK-06344-29 50 PPCO 10,000 Flat top/rim seal Yes 20 bags of 25 500 EK-06344-26 50* PPCO N/A Flat top/rim seal No 20 bags of 25 500 EK-06344-25 50* PPCO N/A Flat top/rim seal Yes 20 bags of 25 500

*Self-standing.


Conical-Bottom Disposable TubesTubes are available in in polystyrene (PS) and polypropylene co-polymer (PPCO)—ideal for applications where other tubes would shatter. The 50-mL tubes 06344-25 and 06344-26 are self-standing with molded-in graduations; all other models feature blue printed graduations and a writing patch. Tubes are available sterile-packed in trays or bags, or nonsterile-packed with caps separate.



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06341-70


Specifications & Ordering InformationBPC material: animal-derived component-free

(ADCF) HyQCX5-14Port 1: 1/8" (3.2 mm) female luer lock,

12" (30.5 cm) tube length

Port 2: 1/8" (3.2 mm) male luer lock, 12" (30.5 cm) tube length

BPC material: animal-derived component-free (ADCF) HyQCX5-14

Port 1: 3/8" (9.5 mm) quick connect insert, 48" (121.9 cm) tube length with 12" (30.5 cm) length dip tube

Port 2: 1/4" (6.4 mm) male luer lock, 48" (121.9 cm) tube length

Port 3: 1/4" (6.4 mm) female luer lock, 48" (121.9 cm) tube length

Port 4: 1/4" (6.4 mm) plugged, 4" (10.2 cm) tube lengthPort 5: 3/8" (9.5 mm) quick connect body,

48" (121.9 cm) tube length, bottom port, silicone

HyClone® Labtainers™ BioProcess ContainersThe only single-use containers that are free of any animal-derived componentsReplace traditional rigid walled plastic and glass tubes, flasks, bottles and carboys with disposable sterile HyClone Labtainers containers. These single-use liquid handling systems minimize the risk of cross contamination, reduce cleaning and turnaround time, making them ideal for biotech and pharmaceutical applications. All products pass USP Class VI medical grade testing. Containers are end-ported and can be hanged for easy dispensing. No dip tubes required to evacuate solutions; no air entrapment or foaming problems. Containers maintains integrity at temperatures ranging from –80 to 60° C. Suitable for packaging, ambient and cryogenic storage of a variety of liquids including media, buffers, process liquids, and biological products. Gamma irradiated, double polybagged.

Single-use disposable liquid handling systems l 3-D geometry conforms better to support containers than traditional pillow-style bagsl Large volume bottom-ported containers allow easy and complete drainage without

air suctionl Medical-grade, animal-derived component-free film manufactured in

a Class 10,000 facilityLarge volume bottom-ported HyQ® containers are specifically engineered for critical fluid handling applications in biomanufacturing. These containers also allow for mixing through a recirculation loop between the top port with diptube and bottom port. All containers are made with HyQ®CX5-14 film and come with a certificate of analysis. The animal derived component-free film passes current USP Class VI medical grade testing. It is also highly compatible with a wide range of buffers and process liquids. Available in 50, 100, and 200 L sizes. End fittings are capped or plugged and protected with a resealable polybag. Tubing lines are C-FLEX® and provided with clamps. Matching high density polyethylene drums is also available for a complete liquid handling system. Gamma irradiated and double polybagged.

HyClone® HyQ® 3-D Single Web™ BioProcess Containers

06341-62

06341-50

06341-56

Catalog number Description Capacity Dimensions (D x H) PriceHyQ containers

KH-06341-50 50 L — KH-06341-52 5 ports, bottom dispense 100 L — KH-06341-54 200 L —

Conical drumKH-06341-56 50 L 233⁄8" x 211⁄2" (59 x 55 cm) KH-06341-58 1 port, bottom dispense 100 L 233⁄8" x 281⁄2" (59 x 72 cm) KH-06341-59 200 L 233⁄8" x 431⁄2" (59 x 110 cm)

DollyKH-06341-62 Plastic drum dolly 50 to 200 L 243⁄8" x 71⁄8" (62 x 18 cm)

Catalog number Description Capacity Qty/pk Price/pkKH-06341-70

2 ports, end dispense50 mL

1

KH-06341-72 100 mL KH-06341-74 250 mL KH-06341-76

2 ports, end dispense500 mL

KH-06341-78 1000 mL KH-06341-80 2000 mL

See more size options of HyClone BioProcess containers.


www.coleparmer.com

ClickHere

http://www.coleparmer.com/catalog/Product_index.asp?cls=44285&referred_id=2269


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Aseptic Sampler SystemInject or withdraw samples in-line without risking contamination from yeast, mold, and other unwanted organismsl Ideal for pharmaceutical, biotech, food, dairy, and fermentation

applicationsl Written instructions can easily be incorporated into

Standard Operating Procedure (SOP) manualsThe aseptic sampler system is a sterile, pressure- and temperature-safe way to handle your sampling needs. The pre-sterilized septum has multiple ports and is designed to allow easy identification of previous penetration points on the septum. This design prevents users from repeatedly penetrating the same point, and therefore eliminates the chance of contamination.

The sanitary fittings allow the user to easily retrofit the aseptic sampler system into pre-existing fluid lines or onto bioreactors. The design of the system prevents the septa from leaking when installed on bioreactors.

The aseptic sampler is easy to install—simply place the septum into the seat on the sanitary fitting, attach the nut onto the fitting, and tighten. Training videos are also available upon request.

6 Composite Sampling BagsUse composite sampling bags in place of a syringe and needle.

KH-96010-74 Composite sampling bag, 2 LKH-96010-76 Composite sampling bag, 5 L

4 Needle KH-07940-70 Needle, 18-gauge 11⁄2". Box of 100For more syringes and needles, see the “Syringe” section at ColeParmer.com.

5 WrenchKH-96010-70 Wrench for 7-port fittingsKH-96010-72 Wrench for 12-port fittings

Aseptic septa cartridge 96010-00 pictured with sanitary fitting assembly 96010-20

Order a Complete Aseptic Sampler SystemKH-96010-80 Aseptic Sampler Kit includes seven disposable syringes, seven sterile needles, seven alcohol wipes, one aseptic sampler and one dust cover. Order sanitary fitting assemblies from above

1 Aseptic septa cartridges (7- or 12-port)2 Sanitary fittings assembly (includes fitting and nut)3 Disposable syringe 4 Needle 5 Wrench

Add this option6 Composite sampling bags, available below with

18"-tubing and needle attached

For a complete selection of stainless steel sanitary fittings, see pages 542–543.

542 to 543

1 Aseptic Septa Cartridges

Catalog number Description Clamp size Price Fittings for 7-port septa

KH-96010-20 Tri-Clamp® elbow with septa seat 11⁄2" KH-96010-22 Tri-Clamp elbow with septa seat 2" KH-96010-24 Weld elbow with septa seat 11⁄2" KH-96010-26 Weld elbow with septa seat 2" KH-96010-28 Tank fitting with nut — KH-96010-30 Insulated tank fitting with nut, bowl — KH-96010-32 Tri-Clamp end cap 11⁄2" KH-96010-34 Tri-Clamp end cap 2"

Fittings for 12-port septaKH-96010-40 Tri-Clamp end cap 2" KH-96010-42 Tri-Clamp end cap 21⁄2" KH-96010-44 Tri-Clamp end cap 3" KH-96010-48 Tri-Clamp end cap 4" KH-96010-50 Weld-on septa holder for non-insulated tanks or 3" pipe — KH-96010-52 Septa holder for insulated tanks —

2 Sanitary Fitting Assemblies (includes dust cover)

Catalog number Number of ports Septa cartridge material PriceKH-96010-00 7 EPDM KH-96010-02 KH-96010-06 12 Silicone and nylon KH-96010-04

Catalog number Capacity Graduations Qty/bx Price/bxKH-07940-30 60 mL 1 mL, 2 oz/in., 1/8 oz 40

3 Disposable Syringe



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Sterilized Sampling Bottle AssembliesSafely transfer, sample, and store biopharmaceutical products and reagents in applications from research to processl Secure, single-piece cap/tubing assembly eliminates

contamination and product loss due to loose connectionsl Assemblies are pre-sterilized by gamma irradiation—eliminates

labor and validation costs associated with assembly and cleaning

l Manufactured in an FDA-registered facility from pharmaceutical-grade resins and tubing

Cap assembly includes a seamless liner with premolded C-FLEX® fill and vent tubes to create an unobstructed fluid path. The 18" fill tube is plugged at the terminal end to maintain assembly sterility until use. Vent tube includes a 0.2-micron Pall® Acrodisc PTFE vent filter to prevent infiltration by contaminants. Vent filter is hydrophobic to guard against loss of costly product or media during filling or emptying. The one-piece liner eliminates the risk of bacterial entrapment and contamination and forms an elastomeric seal between cap and bottle rim.

C-FLEX Tubing is manufactured according to cGMP and meets USP Class VI and USDA requirements. Tubing can withstand temperatures from –100 to 275°F (–73 to 135°C). C-Flex tubing has exceptional tensile and tear strength, extremely low extractables, and excellent biocompatibility. The smooth inner surface reduces absorption and protein binding.

What’s included: Nalgene® clear PETG bottle, 38-mm cap, C-Flex tube/liner assembly with 1⁄8" ID x 1⁄4" OD tubing, and 0.2-micron vent filter.


95644-00

Sterile Tube SealerForms strong, sterile seals in seconds! Securely seal any thermoplastic tubing from 1⁄4" to 11⁄4" OD. Works with liquid-filled tubing—no need to drain before use. Sealer only touches outside of tubing, while inside remains contamination-free. One-button control provides simple, error-free operation. For full information, visit our web site at www.ColeParmer.com.

06480-30

Specifications & Ordering InformationCatalognumber

Dimensions: in. (mm) Max psiat 70°F

Price/25-ft pkID OD Wall

KH-06424-59 1/50 (0.5) 1/12 (2.1) 1/32 (0.8) 30 KH-06424-60 1/32 (0.8) 3/32 (2.4) 1/32 (0.8) 31 KH-06424-62 1/16 (1.6) 1/8 (3.2) 1/32 (0.8) 15 KH-06424-63 3/32 (2.4) 5/32 (4.0) 1/32 (0.8) 14 KH-06424-64 1/8 (3.2) 3/16 (4.8) 1/32 (0.8) 10 KH-06424-67 1/8 (3.2) 1/4 (6.4) 1/16 (1.6) 21 KH-06424-65 5/32 (4.0) 7/32 (5.6) 1/32 (0.8) 10 KH-06424-66 3/16 (4.8) 1/4 (6.4) 1/32 (0.8) 9 KH-06424-03 3/16 (4.8) 5/16 (8.0) 1/16 (1.6) 13 KH-06424-69 3/16 (4.8) 7/16 (11.2) 1/8 (3.2) 22 KH-06424-71 1/4 (6.4) 3/8 (9.6) 1/16 (1.6) 12 KH-06424-72 1/4 (6.4) 7/16 (11.2) 3/32 (2.4) 17 KH-06424-74 1/4 (6.4) 1/2 (12.8) 1/8 (3.2) 17 KH-06424-75 5/16 (8.0) 7/16 (11.2) 1/16 (1.6) 10 KH-06424-76 5/16 (8.0) 1/2 (12.8) 3/32 (2.4) 16 KH-06424-12 3/8 (9.6) 1/2 (12.8) 1/16 (1.6) 10 KH-06424-79 3/8 (9.6) 5/8 (16.0) 1/8 (3.2) 14 KH-06424-19 1/2 (12.8) 11/16 (17.6) 3/32 (2.4) 10 KH-06424-81 1/2 (12.8) 3/4 (19.1) 1/8 (3.2) 10

Applications: Pharmaceutical, biotech, and general-purpose lab uses requiring high performance. Economical alternative to platinum-cured silicone tubing.

Characteristics: Exceptional tensile and tear strength, good biocompatibility, excellent chemical resistance, low gas permeability, and a smooth surface. Opaque white.

Resin certification: Resin meets USP Class VI and USDA requirements. Tubing is manufactured according to GMP.

Temperature range: –100 to 275°F (–73 to 135°C)Sterilize: Autoclave, ethylene oxide, or gamma irradiation.

C-FLEX® Tubing See page

1845.

For a complete selection of tubing suitable for your biotech or pharmaceutical applications.

1845 to 1882

Catalog number Description Power (50/60 Hz) PriceEK-06480-30 Sterile tube sealer 115/230 VAC

Catalog number Description PriceKH-95644-00 125 mL KH-95644-02 250 mL KH-95644-04 500 mL KH-95644-06 1000 mL


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