ENGINEERING MATHEMATICS – III

Academic year 2020-21

III& IV Sem. B.E. Biotechnology, SIT, Tumakuru 1

SCHEME & SYLLABUS OF

III & IV SEMESTERS

B.E. BIOTECHNOLOGY 2020-21

(175 credits scheme)



VISION AND MISSION OF THE DEPARTMENT

VISION:

To be a center of excellence in education and research in Biotechnology

to address the global challenges

MISSION:

1. To establish close collaboration with industry to offer industry-

relevant curricula and pursue demand driven research.

2. To continuously upgrade the infrastructure to develop the state-of-

the-art facilities for training and research.

3. To provide a good learning environment to help students imbibe

professional ethics, communication skills, team spirit and societal

commitment.

4. To offer skill development programs to enhance employability of

graduates in collaboration with industry and Government support.

PROGRAMME EDUCATIONAL OBJECTIVES (PEOs)

Graduates from the Biotechnology program are expected to achieve the

following Program Educational Objectives within a few years of

graduation:

1. The graduates of the program are practicing engineering profession

as clinical data coordinator, clinical research associate, IT system

engineers, bioinformatics analyst, Quality controller and Quality

assurance analyst, business development executive, research

associate with a strong skill set as data analyst, product specialist,

computer programmer, molecular biologist, good planning,

communication and execution ability for IT and BT industries.

2. The graduates of the program are engaged in higher studies leading

to professional degree in specific domain such as biological

sciences, computational biology and also engaged in life-long

learning.

3. The graduates of the program practice profession with high ethical

and moral values and have developed good communication skills

and leadership qualities while working as a member of the team or

as a team leader.



PROGRAM SPECIFIC OUTCOMES (PSOs):

1. Students will be able to conduct the Upstream and Downstream

experiments to produce, optimize, separate, purify and characterize

biological compounds.

2. Students will be able to solve advanced biological problems with the

technical skills of Bioinformatics, Biomolecular simulation,

Proteomics and Genomics using computational techniques.

3. Students will be able to analyze Biopharmaceutical challenges of

Biological systems by applying the concepts of Biological sciences.



PROGRAMME OUTCOMES (POs)

PO1 Engineering knowledge: Apply the knowledge of mathematics,

science, engineering fundamentals, and an engineering

specialization to the solution of complex engineering problems.

PO2 Problem analysis: Identify, formulate, review research literature,

and analyze complex engineering problems reaching

substantiated conclusions using first principles of mathematics,

natural sciences, and engineering sciences.

PO3 Design/development of solutions: Design solutions for complex

engineering problems and design system components or

processes that meet the specified needs with appropriate

consideration for the public health and safety, and the cultural,

societal, and environmental considerations.

PO4 Conduct investigations of complex problems: Use research-

based knowledge and research methods including design of

experiments, analysis and interpretation of data, and synthesis

of the information to provide valid conclusions.

PO5 Modern tool usage: Create, select, and apply appropriate

techniques, resources, and modern engineering and IT tools

including prediction and modeling to complex engineering

activities with an understanding of the limitations.

PO6 The engineer and society: Apply reasoning informed by the

contextual knowledge to assess societal, health, safety, legal and

cultural issues and the consequent responsibilities relevant to

the professional engineering practice.

PO7 Environment and sustainability: Understand the impact of the

professional engineering solutions in societal and environmental

contexts, and demonstrate the knowledge of, and need for

sustainable development.

PO8 Ethics: Apply ethical principles and commit to professional

ethics and responsibilities and norms of the engineering practice.

PO9 Individual and team work: Function effectively as an individual,

and as a member or leader in diverse teams, and in

multidisciplinary settings.



PO10 Communication: Communicate effectively on complex

engineering activities with the engineering community and with

society at large, such as, being able to comprehend and write

effective reports and design documentation, make effective

presentations, and give and receive clear instructions.

PO11 Project management and finance: Demonstrate knowledge and

understanding of the engineering and management principles

and apply these to one’s own work, as a member and leader in a

team, to manage projects and in multidisciplinary environments.

PO12 Life-long learning: Recognize the need for, and have the

preparation and ability to engage in independent and life-long

learning in the broadest context of technological change.



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ENGINEERING MATHEMATICS – III

Contact Hours/Week : 4+0+0 (L+T+P) Credits : 4

Total Lecture Hours : 52 CIE Marks : 50

Course Code : 3RMAT3D SEE Marks : 50

Course objectives: This course will enable students to: 1. Introduce the concept of analytic function, transformation for

mapping. 2. Introduce the concept of complex variables to evaluate the integrals. 3. Introduce the concept of partial differential equations, use

separation of variable method to solve wave, heat and Laplace

equations. 4. Make the student to apply the numerical methods to solve algebraic

and transcendental equations. 5. Solve the system of equation analytically and numerically.

Unit I

Complex Variables: Functions of complex variable, Definition of Limit,

Continuity, Differentiability. Analytic functions, Cauchy’s-Reimann

equation in Cartesian and polar forms (Statement only), Properties of

analytic functions. Geometrical representation f(z)=w, Conformal

transformation: w=ez, w=𝑧 +1

𝑧,w=z2, w=cosh z.

(PO1: M: Knowledge transfer to solve simple engineering problems)

12 Hrs

Unit II

Complex Integration: Bilinear transformation, Properties, Complex

integration, Cauchy’s theorem (statement only), Converse of Cauchy’s

theorem, Cauchy’s integral formula (statement only), zeros &

singularities of an analytic function, residues, residues theorem,

calculation of residues.

(PO1: M: Knowledge transfer to solve simple engineering problems)

9 Hrs

Unit III

Partial differential equations (P.D.E.) : Formation of Partial Differential

Equation, Solution of Langrange’s Linear P.D.E. of the type Pp+Qq=R.

Method of Separation of Variables. Applications of P.D.E.:



Classification of PDE, solution of one dimensional heat wave and two

dimensional Laplace’s equation by the method of separation of variables.

(PO2: M: Identifies all relevant constraints and requirements and

formulates an accurate description of the problem) 11 Hrs

Unit IV

Roots of equation: Introduction, Bracketing methods – Bisection &

Regula-falsi methods. Open methods – Secant method, Newton-Raphson

method, multiple roots and Muller’s method.


formulates an accurate description of the problem) 10 Hrs

Unit V

System of linear algebraic equations: Rank of a matrix by echelon

form, Consistency of a system of linear equations, Gauss elimination

method, Gauss-Jordan method. Iterative methods – Jacobi and Gauss-

Seidel iterative methods.


formulates an accurate description of the problem)

10 Hrs

TEXT BOOKS:

1 B.S.Grewal “Higher Engineering Mathematics”, Khanna Publications,

43rd Edition, 2015, 978-8193328491

2 B.S.Grewal “Numerical Methods”, Khanna Publications, 43rd Edition,

2014, 978-8174092489

REFERENCE BOOKS:

1 Erwin Kreyszig “Advanced Engineering Mathematics”, Wiley

Publications,10rd Edition, 2015, 978-

8126554232

2 C. Ray Wylie and

Louis C. Barrett

“Advanced Engineering Mathematics”, Tata-

McGraw Hill, 6th Edition, 2005, 978-

8177585469

3 Louis A. Pipes and

Lawrence R. Harvill

“Applied Mathematics for Engineers and

Physicists”, McGraw Hill, 3 Edition, 2014, 978-

0486779515



Course Outcomes: After the completion of this course, students will be

able to:

1. Apply basic mathematical operations on complex numbers in

Cartesian and polar forms. Determine

continuity/differentiability/analyticity of a function and find the

derivative of a function. Identify the transformation. (BL: L1, L3)

(PO1-M)

2. Evaluate a contour integral using Cauchy’s integral formula.

Compute singularities and also the residues. (BL: L3) (PO1-M)

3. Formulate and solve partial differential equations. Use of

separation of variable method to solve wave, heat and Laplace

equations. (BL: L4) (PO2-M)

4. Compute the roots of an algebraic or transcendental equation.

(BL: L3) (PO2-M)

5. Solve the system of equation analytically and numerically.(BL: L3)

(PO2-M)

CORRELATION BETWEEN COURSE OUTCOMES WITH PROGRAM

OUTCOMES Program articulation matrix

Course PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RMAT3D 2 2

Mapping of course outcomes with program outcomes

Course outcomes

Program outcomes (POs)and Program specific

outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Apply basic mathematical operations on complex numbers in Cartesian and polar forms. Determine continuity/differentiability/analyticity of a function and find the derivative of a function. Identify the transformation.

2



Evaluate a contour integral using Cauchy’s integral formula. Compute singularities and also the residues.

2

Formulate and solve partial

differential equations. Use of

separation of variable method to solve

wave, heat and Laplace equations

2

Compute the roots of an algebraic or transcendental equation

2

Solve the system of equation analytically and numerically 2

1: Low, 2: Medium, 3: High



MICROBIOLOGY

Contact Hours/Week : 4 +0+0 (L+T+P) Credits : 4


Course Code : 3RBT01 SEE Marks : 50

Course objectives: This course will enable students to:

1. Study basic structure of prokaryotes and eukaryotes, concepts of

isolation, culturing of microorganisms.

2. Staining of microorganism and to study the various types of microbes.

3. Study growth and reproduction in bacteria, preservation of

microorganisms.

4. Enumeration of soil, water and air microorganisms and control of

microorganisms.

5. Understanding general characteristics of virus, its classification &

reproduction of viruses.

Unit I

Introduction: History of microbiology, Scope of microbiology and

branches, Origin of life: biogenesis and abiogenesis. Major contributions

of Antony Van Leeuwenhoek, Louis Pasteur, Alexander Fleming, Robert

Koch and Joseph Lister. Prokaryotes & Eukaryotes: Distinguishing

features with diagrammatic illustrations. Types of microorganisms -

Morphology and fine structure of Bacteria, viruses, Fungi, Algae,

protozoa.

Culturing of microorganisms: Bacteria, Culturing of bacteria,

Nutritional requirements – physical and chemical, Different types of

media – Solid, semisolid and broth; synthetic media, Special media-

blood agar, Selective media, complex media, indicator media, differential

media, transport media; anaerobic media- (thioglycolate, Robertson’s

media, micro aerophilic). Composition and preparation of basic culture

media - Nutrient agar, EMB, Mc.Conkey and blood agar. Pure culture

techniques – Serial dilution method, pour plate, spread plate, streak

plate, lawn culture and stab culture and cultural characteristics (colony

characteristics) – shape, size, color, elevation, texture, margin,

consistency, pigmentation and optical features. Episome (F, R, Ti as

example); Bergey’s Manual of Systematic Bacteriology, Molecular



approaches to Taxonomy- Taxonomic classification of microbes using

molecular markers- 16s rRNA typing; taxonomic implications of DNA

base composition. Concepts of Anesthesia and euthanasia. (PO1-

LKnowledge transfer: Knowledge of basic science).

12 Hrs

Unit II

Microscopy: Study of microscopes and its types – construction, working

principle, working method and its applications; Bright Field Microscopy,

Dark-Field Microscopy, Phase Contrast Microscopy, Fluorescence

Microscopy, Electron microscopy (SEM, TEM) (PO5-L Modern tool

usage).

Staining of Microorganisms:

Types of Stains- Acidic, Basic and Neutral Stain. Theories of Staining;

Physical - Absorption, Osmosis and Capillary and Chemical - Ion

Exchange Method. Staining of microorganisms-simple staining, negative

staining, Differential Staining-Gram staining and Acid-Fast Bacilli

Staining, structural staining (endospore, capsule, flagella). (PO1-L

Knowledge transfer: Knowledge of basic science)

10 Hrs

Unit III

Reproduction and Growth of Microorganisms: Reproduction in

Prokaryotes; modes of Cell Division- Binary Fission, Budding and

Fragmentation, Continuous culture-Chemostat, Synchronous culture,

Direct and Indirect measurement of growth, Factors affecting growth -

Nitrogen content, Turbidometric, Nucleic Acid content etc. Growth

principles of nutrition, influence of Environmental factors-pH,

Temperature, Oxygen, Heavy metals and other compounds, Oxygen

toxicity: Study of catalase, peroxidase, superoxidase dismutase,

mechanism of oxygen toxicity;

Maintenance of cultures– periodic transfer, using mineral oil,

lyophilization and by low temperature. (PO1-M Knowledge transfer:

Knowledge of basic science). 10 Hrs

Unit IV

Enumeration of microorganisms:

Agar-Plate culture technique, Direct microscopic examination, by

enrichment culture technique, streak plate method. Soil microorganisms



– Bacteria, Fungi, Algae, Protozoa and Viruses. Interaction among soil

microorganisms-symbiosis, mutualism, commensalism, antagonism,

competition, parasitism and predation. (PO1-LKnowledge transfer:

Knowledge of basic science)

Control of Microorganisms: Definition used in Microbial control

methods, the pattern of microbial death.

Sterilization methods:

Physical methods: Heat - dry heat – flaming, red hot and hot air oven,

moist heat- Pasteurization, autoclave, tyndallization, low temperatures,

filtration, radiation – ionizing and non-ionizing.

Chemical methods: phenolics, alcohols, halogens, heavy metals,

quaternary ammonium compounds, aldehydes, Sterilizing gases,

chemotherapeutic agents. (PO1-LKnowledge transfer: Knowledge of

basic science, PO8-L professional ethics and responsibilities)

Other Methods: Broad and narrow spectrum antibiotics and mode of

action, anti-fungal and antiviral methods.

Microbial pathogens and Pathogenesis: Common diseases caused by

microbes; etiology, transmission and symptoms of the disease - Bacterial

diseases: Typhoid, Tuberculosis, Cholera; Fungal diseases: Candidiasis.

Protozoan diseases: Malaria, Gonorrhea. (PO6-M Knowledge to health)

10 Hrs

Unit V

Viruses: Morphology, Classification and Replication: General

Characteristics of viruses: Basic morphology of viruses - Helical capsids,

icosahedral capsids, virus with capsids of complex symmetry, viral

envelopes and enzymes, virion size, genetic material, other chemical

components; replication of viruses. Bacteriophages as therapeutic

agents; morphology and chemical composition of bacteriophages.

Classification and nomenclature of bacteriophages: system of

classification based on differences in transcriptional process;

Bacteriophages of E.coli. Bacteriophage life cycle: The lytic Life cycle -

Virulent phages. The lysogenic Life Cycle-Temperate phages; Cultivation

of viruses, Principles of viral taxonomy. Viroids and Virusoids, Prions.

Viral diseases: Ebola, Hepatitis, Herpes, Polio; Zika, COVID-19. (PO1-L



Knowledge transfer: Knowledge of basic science,PO8-L professional

ethics and responsibilities)

10 Hrs

TEXT BOOKS:

1 Stanier, John Ingraham, Mark Wheelis.

General Microbiology, Mac- Millan Pub. Published by Macmillan, Hampshire & London, 5th Edition, 1992, 978-0299108243

2 Michael. J. Pelczar, Chan , Krieg

General Microbiology, W C Brown Pub. Published by Tata McGraw-Hill

Education Pvt. Ltd, 7th Edition, 2007, 978-9350240892

3 Michael T. Madigan, John M.

Martinko, Kelly S. Bender, Daniel H. Buckley, David A. Stahl, Thomas Brock

Brock Biology of Microorganisms,

Benjamin Cummings, 14th Edition, 2014, 978-0132192262

REFERENCE BOOKS:

1 Gerard J, Tortora, Berdell

R Funke, Christine L Case

Microbiology: An Introduction, Pearson

Education, 11th Edition, 2008, 978-

9332575417

2 Ananthanarayan and

Paniker

Textbook of Microbiology, Universities

Press, 8th Edition, 2009, 978-

0199681686

3. Power and Daginawala General Microbiology: Himalaya

Publishing House Pvt. Ltd., Vol I and Vol

II, 5th Edition, 2009, 978-9350240892

Course Outcomes: After the completion of this course, students will be able

to:

1. Distinguish physiological and morphological features of

microorganisms and explain types of media preparation and

culture techniques. And also outline the taxonomy of microbes.

(BL: L1, L2) (PO1-L)

2. Identify microorganisms using microscopy and staining methods.

(BL: L2) (PO1-L, PO5-L)



3. Explain the reproduction, factors affecting growth of

microorganisms and preservation methods. (BL: L2) (PO1-M)

4. Illustrate microbial techniques to isolate, identify and enumerate

the microorganisms. Describe the methods for control of

microorganisms and diseases. (BL: L2) (PO1-L, PO6-M, PO8-L)

5. Explain the morphology, life cycle, characteristics, classification of

viruses and diseases. (BL: L2) (PO1-L, PO8-L)

CORRELATION BETWEEN COURSE OUTCOMES WITH PROGRAM OUTCOMES

Program articulation matrix

Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBT01 1 1 1 1 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Distinguish physiological and morphological features of microorganisms and explain types of media preparation and culture techniques. And also outline the taxonomy of microbes

1

2

Identify microorganisms using microscopy and staining methods.

1 1 2

Explain the reproduction, factors

affecting growth of microorganisms

and preservation methods.

2

2

Illustrate microbial techniques to isolate, identify, enumerate the microorganisms. Describe the methods for control of microorganisms and diseases.

1 2 1

2

Explain the morphology, life cycle, characteristics, classification of viruses and diseases.

1 1 2




BIOCHEMISTRY Contact Hours/Week : 4+0+0 (L+T+P) Credits : 4



Course Objectives: This course will enable students to: 1. Learn about biochemical and physical properties of carbohydrates

and lipids.

2. Understand the basic structural, chemical and physical properties

of biomolecules namely proteins and nucleic acids.

3. Know the fundamentals of photosynthesis reaction and energy

transfer in biological system.

4. Understand the metabolism of carbohydrates and lipids in

biological system.

5. Understand the metabolism reactions of proteins and nucleic acids.

Unit I

Carbohydrates and Lipids:

Basic Concepts: Biochemistry: Definition, Introduction to Biochemistry,

Introduction to Biomolecules (Carbohydrates, Lipids, Proteins, DNA and

RNA) with relevance to Biochemistry and its Functional aspects.

Carbohydrates:

Carbohydrates: Introduction, sources, three major size classes of

carbohydrates (mono, di and polysaccharides), classification of

monosaccharides based on number of C-atoms (classification based on

functional groups: aldoses and Ketoses). Structural aspects of sugars:

pyranose and furanose structures, reducing and non-reducing sugars,

anomers. Structural and functional aspects of biologically important

sugars: maltose, isomaltose, lactose, cellulose, cellobiose, trehalose,

sucrose, raffinose, starch, glycogen, chitin.

Lipids:

Introduction, definition, types: storage, membrane and structural lipids

(structure aspects and properties of each lipid). (P01-L: Knowledge

transfer: Knowledge of basic science)

10 Hrs



Unit II

Proteins and Nucleic acids:

Basic Concepts:

Introduction to proteins, nucleic acids and hormones.

Proteins:

Amino acids (Structure, classification, and properties of amino acids).

Structural organization: Primary structure, Secondary structure, tertiary

structure and quaternary structure of proteins. Important concepts

related to amino acids: Zwitter ions, pka and isoelectric point. Types of

proteins: Globular, enzymatic, structural, transport proteins and others

(Functional aspects only).

Nucleic acids:

Definition, purine & pyrimidines, nucleosides, nucleotides of DNA &

RNA, base pairing, structure of DNA, Types of DNA, Structure of RNA

and types of RNA (m-RNA r-RNA, t-RNA).

Hormones

Biological important hormones – vasopressin, oxytocin, erythropoietin.

(P01-L: Knowledge transfer: Knowledge of basic science)

12 Hrs

Unit III

Bioenergetics : Energy flow cycle and energy conversions. Structure &

properties of ATP, GTP, FAD& NADP. Photosynthesis: Stages in

Photosynthesis light reactions & dark reactions. Light reactions-

structure of chlorophyll, light harvesting complexes, photo systems

PSI&PSII, photophosphorylation, ancillary pigments. Dark reactions-

Calvin cycle. Electron transport chain and oxidative phosphorylation:

production of ATP, Energetics, Energy balance sheet, oxidative stress.

(P01-M: Knowledge transfer: Knowledge of basic science)

10 Hrs

Unit IV

Metabolism & its regulation:

Carbohydrate Metabolism: Glycolysis- Metabolism & its regulation,

aerobic and anaerobic pathway, TCA cycle, amphibolic role, anaplerotic

reactions of TCA cycle. Glyoxylate cycle, PP pathway & its regulation,



gluconeogenesis and regulation. Biosynthesis & degradation of

polysaccharides (glycogen).

Lipid Metabolism: Oxidation of fatty acids- α, β and ω types, energetics

of β-oxidation. Biosynthesis of even number saturated fatty acids,

Cholesterol biosynthesis. (P01-M: Knowledge transfer: Knowledge of

basic science) 10 Hrs

Unit V

Amino acid and nucleic acid metabolism: Biosynthesis of amino acid

starting from acetyl CoA (with reference to Oxaloacetate family)-

Asparagine, Threonine, Methionine, and Lysine. Biodegradation of amino

acids, deamination, transamination & urea cycle. Nucleic acid

Metabolism Biosynthesis, biodegradation and regulation of Purines,

Pyrimidines and nucleic acids. (P01-M: Knowledge transfer:

Knowledge of basic science). 10 Hrs

TEXT BOOKS:

1 Nelson & Cox. Lehninger’s Principles of Biochemistry, W.H

Freeman and Company, New York, 5th Edition,

2008, 978-1319108243

2 U. Satyanarayana

and U. Chakrapani

Biochemistry, Books and allied (p) Ltd.

Kolkata, 4th Edition, 2008, 978-9351529255

REFERENCE BOOKS:

1 Stryer Biochemistry, W.H Freeman and company,

5th Edition, 2010, 978-1319026455

2 Donald Voet, Charlotte

W. Pratt, Judith G. Voet

Principles of Biochemistry, Wiley

Publication, 4th Edition, 2013, 978-

1319108243


able to:

1. Memorize and classify the biological molecules like carbohydrates and

lipids based on structural and functional groups. (BL: L1, L2) (PO1-L)

2. Memorize and classify the biological molecules like Proteins and nucleic acids based on structural and functional groups, further to discuss the importance of biologically important peptides. (BL: L1, L2) (PO1-L).



3. Explain the concepts of cell energetics by memorizing the knowledge

of Energy flow cycle and energy conversions. (BL: L2) (PO1-M)

4. Describe the concepts of carbohydrate and lipid metabolism and

explain the products of metabolism. (BL: L2) (PO1-M)

5. Describe the concepts of protein and nucleic acid metabolism and

explain the products of metabolism. (BL: L2) (PO1-M)



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBT02 2 2


Course outcomes

Program outcomes (POs)and Program specific outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PS

O1

PS

O2

PS

O3

Memorize and classify the biological molecules like carbohydrates and lipids based on structural and functional groups.

1 2

Memorize and classify the biological molecules like Proteins and nucleic acids based on structural and functional groups, further to discuss the importance of biologically important peptides.

1 2

Explain the concepts of cell energetics

by memorizing the knowledge of Energy

flow cycle and energy conversions.

2 2

Describe the concepts of carbohydrate and lipid metabolism and explain the products of metabolism.

2 2

Describe the concepts of protein and nucleic acid metabolism and explain the products of metabolism.

2 2




BIOPROCESS CALCULATIONS

Contact Hours/Week : 3+0+0 (L+T+P) Credits : 3.0



Course Objectives: This course will enable students to:

1. Introduce the principles and calculation techniques in bioprocess

engineering.

2. Acquaint students with material and energy balance calculations.

3. Teach basic calculation techniques in bioprocesses involving cell

growth and biochemical reactions.

4. Illustrate the significance of bypass, recycle, and purging

operations in bioprocess engineering and introduce relevant

calculations.

5. Introduce the concept of unsteady-state material and energy

balance in bioprocess engineering and relevant calculations.

Unit I

Units, Dimensions and Basic Chemical Calculations: Steps in a typical

Bioprocess development and the need for engineering calculations.

Introduction to engineering calculations: Physical variables, units and

dimensions - Substantial and natural variables: volume, temperature,

pressure, force, weight, density/specific gravity, specific volume, flow

rate, mole, chemical composition, heat. Measurement conventions.

Dimensional homogeneity in equations, equations without dimensional

homogeneity. Unit conversion, conversion of equations. Standard

conditions and ideal gases. (PO1-H Knowledge transfer, PO2-M

Problem analysis, PO3-M Development of solutions to engineering

problems, PO9-M Individual/Team work, PO10-M Scientific

Communication)

8 Hrs

Unit II

Presentation and Analysis of Bioprocess Data: Errors in data and

calculations: Significant figures, absolute and relative uncertainty,

propagation of errors, types of errors, statistical analysis. Presentation of

experimental data. Bioprocess data analysis - Trends, testing



mathematical models, Least-squares analysis, linear/nonlinear models.

Mathematical procedures: Trial-and-error method, graphical method,

graphical integration, log-log plots, semi-log plots, triangular plots.

General procedures for plotting data. Bioprocess flow diagrams. (PO1-H

Knowledge transfer, PO2-M Problem analysis, PO3-M Development of

solutions to engineering problems, PO9-M Individual/Team work,

PO10-M Scientific Communication)

6 Hrs

Unit III

Material balance without reaction: Basic thermodynamic concepts -

System, process, steady state and equilibrium. Law of conservation of

mass. Introduction to material (mass) balance, types of material balance

(differential & integral and total & component), concept of basis and tie

components. Guidelines for material balance calculations. Material

balance for mixing, filtration, extraction, evaporation, distillation,

membrane separation and crystallization. Material balances with recycle,

bypass, and purge streams. Unsteady-state material balance without

reaction. (PO1-H Knowledge transfer, PO2-M Problem analysis, PO3-M

Development of solutions to engineering problems, PO9-M

Individual/Team work, PO10-M Scientific Communication)

8 Hrs

Unit IV

Material balance with biochemical reaction: Stoichiometry concepts:

Limiting and excess reactants, fractional and % conversion, Yield

(fractional and %), extent of reaction and selectivity. Stoichiometry of cell

growth and product formation - Growth stoichiometry and elemental

balances, electron balances, biomass yield, product stoichiometry,

theoretical oxygen demand, maximum possible yield. Material balances

with recycle, bypass, and purge streams with reaction. Unsteady-state

material balance with biochemical reaction. (PO1-H Knowledge

transfer, PO2-M Problem analysis, PO3-M Development of solutions

to engineering problems, PO9-M Individual/Team work, PO10-M

Scientific Communication)

8 Hrs

Unit V

Energy balance: Basic energy concepts - Intensive and expensive



properties, enthalpy. General energy balance equation. Procedures of

enthalpy calculation. Enthalpy change in nonreactive processes - change

of phase, mixing and solution. Energy balance calculations without

reactions. Enthalpy change due to reaction - Heat of combustion, heat of

reaction. Heat of reaction for processes with biomass production -

thermodynamics of cell growth, heat of reaction with and without oxygen

as electron acceptor, mixed aerobic-anaerobic metabolism, heat of

reaction in different cell cultures. Energy balance equation for cell

culture. Unsteady-state energy balance with and without biochemical

reaction. (PO1-H Knowledge transfer, PO2-M Problem analysis, PO3-M

Development of solutions to engineering problems, PO9-M

Individual/Team work, PO10-M Scientific Communication)

9 Hrs

TEXT BOOKS

1 Pauline M.

Doran

Bioprocess Engineering Principles, Prentice-Hall

Inc., 2nd Edition, 2013, ISBN: 978-0-12-220851-5

2 Shuler M L,

Kargi & DeLisa

M P

Bioprocess Engineering – Basic Concepts, Prentice-

Hall Inc., Upper Saddle River, NJ, 3rd Edition, 2017,

ISBN: 978-0-13-706270-6

REFERENCE BOOKS

1 Himmelblau, D.M.

and Riggs, J.B.

Basic Principles and Calculations in Chemical

Engineering, PHI Learning Pvt. Ltd., Connaught

Circus, New Delhi, 7th Edition, 2009, ISBN: 978-

81-203-3839-5

2 Narayanan, K.V.,

Lakshmikutty, B.

Stoichiometry and Process Calculations. PHI

Learning Pvt. Ltd., Connaught Circus, New

Delhi, 1st Edition, 2011, ISBN: 978-81-203-

2992-8




able to:

1. Demonstrate the knowledge of calculations in chemistry, physics,

biology and mathematics and apply them to solve basic biochemical

engineering unit operations and processes.

(BL: L1, L2,) (PO1-L, PO9- M, PO10-M)

2. Analyze problems related to bioprocess calculations and provide

conclusions using the first principles of material and energy balance.

(BL: L1, L2, L3) (PO1-L, PO2-M, PO9-M, PO10-M)

3. Develop solutions to basic and complex bioprocess calculations

problems. (BL: L1, L2, L3) (PO1-L, PO2-M, PO3-M, PO9-M, PO10-M)

4. Communicate the solutions to bioprocess calculations problems

effectively in both oral and written form. (BL: L1 - L5) (PO1-L, PO2-M,

PO3-M, PO10-M)

5. Demonstrate the ability of identify, analyze and solve bioprocess

calculation problems individually and in a team. (BL: L1 - L5) (PO1-L,

PO2-M, PO3-M, PO9-M)





Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBT03 3 3 2 2 2


Course outcomes


PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Demonstrate the knowledge of

calculations in chemistry, physics,

biology and mathematics and apply

them to solve basic biochemical

engineering unit operations and

processes

3 3

Analyze problems related to

bioprocess calculations and provide

conclusions using the first

principles of material and energy

balance

3 3 2 3

Develop solutions to basic and

complex bioprocess calculations

problems

3 3 2 3

Communicate the solutions to

bioprocess calculations problems

effectively in both oral and written

form

3 3 2 2 2

Demonstrate the ability of identify,

analyze and solve bioprocess

calculation problems individually

and in a team

3 3 2 2 2

1:Low, 2: Medium, 3: High



TRANSPORT PROCESSES - I

Course Objective: This course will enable students to:

1. Describe the concepts of fluid flow and rheological properties of fermentation broths.

2. Learn the basic equations involved in flow of fluid through circular pipes.

3. Learn the basic equations used in different types of flow meters like orificemeter, venturirmeter and pumps and its operation.

4. Learn various parts and flow patterns in mixing equipment. 5. Learn the various equations involved in the power calculation of

mixing equipment.

Unit I

Momentum transfer: Introduction to momentum transfer, properties of

fluids, measurement of density and specific gravity, classification of

fluids, Shear stress, Viscosity, Newtonian and Non-Newtonian fluids,

Two-parameter models, Time-dependent viscosity, Viscosity

measurements- Cone- and –Plate viscometer, Coaxial cylinder

viscometer, Impeller viscometer, Use of viscometers in fermentation

broths, Rheological properties of fermentation broths, Factors affecting

broth viscosity. Concepts of fluid flow: Types of flow, Reynold’s number

and its significance, continuity equation and applications of Bernoulli’s

equation and numerical problems. (PO1: M: Apply the knowledge of

mathematics, science, engineering fundamental) 8 Hrs

Unit II

Fluid Flow in conduits and piping system: Flow of in-compressible

Fluids in Conduits and Thin Layer: Estimation of pressure drop in

Newtonian fluids under laminar flow (Hagen Poiseulle’s equation) Friction

factor and its significance, Moody’s diagram and estimation of friction

factor. Numerical problems. Concepts of Hydrodynamic boundary layers

and Boundary layer separation. Nature of Turbulence, representation of

turbulent flow by mean and fluctuating velocities and correlations of

turbulent flow. Piping system: Definition and types of pipes, schedule






number, types of pipe fittings and their role, Types of valves and their

construction. (PO2: M: Identify and analyze complex engineering

problems reaching substantiated conclusions using first principles

of mathematics ) 8 Hrs

Unit III

Flow measuring devices: Types of pressure, introduction to pressure

gauge and pressure transducers. Measurement of fluid flow rates by

orifice meter, venturimeter, rotameter, pitot tube, wet gas flow meter and

magnetic flow meters- construction and working. Numerical problems on

venturimeter and orificemeter. Principles of flow visualisation by particle

image velocitymetri and laser doppler anemometer. Transfer of fluids by

pumps: -construction and working of centrifugal, reciprocating and

peristaltic pumps. Operating curves of centrifugal pumps. (PO2: M:

Identify, review research literature and analyze complex

engineering problems reaching substantiated conclusions using

first principles of mathematics) 7 Hrs

Unit IV

Mixing: Functions of mixing, Mixing equipment- Vessel geometry and

liquid height, Baffles, Sparger, Stirrer shaft, Flow patterns in stirred

tanks –Rotational flow, Radial flow, Axial flow, Gas flow patterns,

Impellers –Rushton turbine –with gassing, Without gassing, Propellers,

Pitched blade turbine, Alternative impeller designs-Curved blade disc

turbines, Hydrofoil impellers. (PO1: M :Apply the knowledge of science

and engineering fundamentals) 8 Hrs

Unit V

Power requirements: Ungassed Newtonian fluids, Ungassed Non-

Newtonian fluids, gassed fluids, power input by gassing, Impeller

pumping capacity, Suspension of solids, Mechanisms of mixing,

Assessing mixing effectiveness, Scale-up of mixing in fermenters,

Multiple impellers, Retrofitting, Effect of rheological properties on mixing,

Role of shear in stirred fermenters-Studies with animal cell cultures, Cell

damage from bursting bubbles.

(PO1: H : Apply the knowledge of mathematics, science, engineering

fundamentals). 8 Hrs



TEXT BOOK:

1 Pauline M. Doron

Bioprocess Engineering Principles, Elsevier, 2nd Edition, 2013, 978-0-12-220851-5

REFERENCE BOOK:

1 Coulson J. II and

Richardson J.F.

Chemical Engineering.Vol.1, Asian Books. New Delhi, Robeth LLC, 6th Edition, 1998, 818147144X

2 McCabe W L et. al.

Unit Operations of Chemical Engineering, McGraw Hill New York, Marcel Dekker Inc, 7th Edition,

1993, 0071247106


able to:

1. Apply the basic concepts of fluid and analyze flow systems based on

continuity, Bernoulli’s equations for mass and energy balances.

2. Estimate the pressure drop and friction factor of a Newtonian fluid

flowing through a circular pipe.

3. Demonstrate the knowledge on transportation of fluids, transport and

flow measuring devices with regard to principles, construction and

working.

4. Understand various parts of mixing equipment, functioning of mixing

and flow patterns in mixing.

5. Analyze the power required in mixing processes with ungassed

Newtonian and non-Newtonian fluids.



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBT04 2,3 2,3 1,2




Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Apply the basic concepts of fluid

and analyze flow systems based on continuity, Bernoulli’s equations for mass and energy

balances

3 1

Estimate the pressure drop and

friction factor of a Newtonian fluid flowing through a circular pipe

2 3 2

Demonstrate the knowledge on transportation of fluids, transport and flow measuring devices with

regard to principles, construction and working

2 2 2

Understand various parts of mixing equipment, functioning of mixing and flow patterns in

mixing

2 2

Analyze the power required in

mixing processes with ungassed Newtonian and non-Newtonian fluids

2 2

1: Low, 2: Moderate, 3: High



GENETICS Contact Hours/Week : 3+0+0 (L+T+P) Credits : 3




1. Study the Structural and functional aspect of Eukaryotic and

prokaryotic cells.

2. Study the Structure and Function of Biological Macromolecules like

DNA and RNA and the work of Mendel.

3. Study the Principles underlying Genetic Interaction and to analyze

Genotypic and Phenotypic Variations.

4. Know about the Sex-Linked Inheritance in Humans.

5. Learn about Genetic Code and Gene Mutation: Variants, Effects.

Unit I

Introduction to cell: Eukaryotic and prokaryotic cells, Plant and animal

cells, brief mention of membrane organization. Cell structure and

function: cell division- Mitosis and Meiosis. Structure of cytoplasm,

Nucleus, Mitochondria, Ribosome, Golgi bodies, Lysosomes. Endoplasmic

Reticulum, Peroxisomes, Chloroplast and Vacuoles. Cell to cell

integration, Cell locomotion (Amoeboid, Flagella, Cilla). (P01-L:


8 Hrs

Unit II

Chromosomes: Journey from Nucleic Acid to Chromosomes, Ultra

structure of Chromosomes, Palindromic DNA and Satellite DNA. Polytene

and Lamp-Brush Chromosomes, Human Chromosome and its analysis,

aims and objectives Human Genome Project.

Genetics and Mendel concepts: Terminologies; Gene, Allele or

Allelomorph, Dominant and Recessive, Homozygote and Heterozygote,

Phenotype and Genotype, Carriers, Genetic symbols. Mendel’s work,

Mendel’s Laws, Monohybrid, Dihybrid, Trihybrid and Multi-hybrid cross,

Test cross, Backcross. Other Allelic Relationships; Co-dominant allele,

Incomplete dominance, Lethal Allele, Multiple allele. (P01-H: Knowledge



transfer: Knowledge of Mathematics, basic science to solve simple

problems) 8 Hrs

Unit III

Genetic Interactions: Two factor interaction; 1) Epistatic Interaction:

Dominant Epistasis, Recessive Epistasis, Duplicate Genes with

Cumulative Effect, Duplicative Dominant Genes, Duplicate Recessive

Genes, Dominant and Recessive Interactions. 2) Non-Epistatic

Interactions. Three or more Factor Interactions. The Genetics of Sex:

The Importance of Sex. Sex determining mechanisms: 1) Sex

Chromosome mechanism; Heterogametic males (XX-XY, XX-XO),

Heterogametic Females (ZW-ZZ, ZO-ZZ) 2) Genic Balance (Drosophila

Melanogaster) 3) Haplodiploidy 4) Single Gene effects. (P01-M:

Knowledge transfer: Knowledge of basic science).

8 Hrs

Unit IV

Sex-Linked Inheritance: Sex-linked Inheritance; The Organ of Heredity,

Chromosomes, Morphology, Classification (only X and Y chromosomes).

Chromosomal disorders. Sex Linked Inheritance: Molecular Diseases,

Hemoglobin pathies. Disorders of Coagulation, Color Blindness,

Hemophilia. Linkage and Recombination: Phases of Linkage, Linkage

Groups; Complete and Incomplete, four strand Crossing over, Detection

of Linkage. Crossing Over: Crossing Over (Essential Features, Kinds,

Mechanism), Cytological Basis of Crossing Over; Stern’s Experiment in

Drosophila, Creighton and Mc-Clintock experiment in Maize. (P01-M:


8 Hrs

Unit V

Gene mutation and population genetics: Genetic Codes and Gene

Mutation: Definition, Classification, Types of Mutagens – Chemical and

Physical Mutagens of Mutation, Mechanisms of Mutation, Base Pairing

Substitution, Frame Shift Mutation Missense Mutations, Nonsense

Mutations, Mutation in Termination Codons, Silent Mutations, Genetic

Suppression, Kinds of mutations – SNPs, jumping genes. (P01-H:

Knowledge transfer: Knowledge of basic science). Molecular Basis of

Mutation, Radiation. Gene Frequency and Equilibrium Estimation,

Changes in Gene Frequency, Genetic Structure of Population, Speciation



and Evolution, Prospects for The Control of Human Evolution. Numerical

Problems Eugenics. Pedigree Analysis (P01-M: Knowledge transfer:

Knowledge of basic science and mathematics). Case study: Sickle

cell anaemia. Polymorphism. Wright-Fisher model. (P02-L: To gather

knowledge on genetic aberrations from the open literature)

7 Hrs

TEXT BOOKS:

1. Susan Elrod,

William Stansfield.

Genetics, Schaum’s Out lines, 3rd Edition,

1991, 978-0071625036

2. Gardner, Simmons,

Snustad.

Principles of Genetics, Wiley India, 6th

Edition, 2006, 978-8126510436

3. Darnell J Lordes and H

Balitimore.

Molecular cell Biology, Scientific American

books USA, 4th Edition, 2015, 978-

0815344643

REFERENCE BOOKS:

1. Monroe W Strickberger. Genetics, Phi learning, 3rd Edition, 2008,

978-9332555105

2. P.C.Winter, G.I.Hickey

and H.L.Fletcher.

Instant notes in Genetics, Taylor and Francis

Publishers, 10th Edition,1999, 978-

0415693141

Course Outcomes: After the completion of this course, students will be able to:

1. Classify the type of cell organelles and their functions. (BL: L2)

(PO1-L)

2. Describe the Structure & Function of DNA, RNA and Explain

Mendelian Laws of Genetics. (BL: L2) (PO1-H)

3. Explain the Genetic Interaction, Analyse Genotypic & phenotypic

Variations. (BL: L2, L4) (PO1-M)

4. Describe how Sex influences the Trait of an Organism. (BL: L2)

(PO1-M)

5. Distinguish the Features of Genetic Code and Analyse the

Mutations in Genes and gene population. (BL: L2, L4) (PO1-H,M,

PO2-L)




OUTCOMES


Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBT05 2 1 2


Course outcomes

Program outcomes (POs)and Program

specific outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Classify the type of cell organelles and their functions.

1 2

Describe the Structure &

Function of DNA, RNA and Explain Mendelian Laws of

Genetics.

3 2

Explain the Genetic Interaction,

Analyze Genotypic & phenotypic Variations.

2 2

Describe how Sex influences the Trait of an Organism.

2 2

Distinguish the Features of

Genetic Code and Analyze the Mutations in Genes and gene

population.

1 1 2




MICROBIOLOGY LABORATORY

Lab Hours/ Week : 0+0+3 (L+T+P) Credits : 1.5

Course Code : 3RBTL01 CIE Marks : 50

SEE Marks : 50

Course objectives: This course will enable students to: 1. Expertise one with basic strategies of isolation, culturing and

preservation of microorganisms.

2. Make proficient enough with basic staining of microorganism to

know the kind of microbes.

3. Get well acquainted with handling of essential instruments used in

a microbiology lab; face and solve the trouble shoots.

4. Understand manual and automated enumeration techniques of

soil, water and air microorganisms.

5. Acquire ability to identify the potability of different daily essential

consumables.

List of Experiments:

1. Introduction: a. Aseptic techniques – Hot air oven,

Autoclave,membrane filter, Laminar airflow, Incubator. b.Cleaning of glassware’s.

2. Media preparation – a. Broth and Agar b. Instrumentation, Handling

and care of Microscope.

3. Isolation of microorganism from soil sample by serial dilution method

(Morphology - Bacteria, fungi, yeast - Mounting and identification.)

Pour plate method, B. Streak plate method, C. Spread plate method.

4. Isolation of microorganism from rotten fruits and vegetables by serial

dilution method. (Morphology - Bacteria, fungi, yeast - Mounting and

identification)

5. Examination of microorganisms from tooth scrapings. (PO1-L Knowledge transfer: Knowledge of basic techniques in

microbiology, PO4-M Investigation methodology, analyses of

data considering sources of error and draws valid conclusions,

PO6-L Imbibing professional engineering practices, PO8-M

professional ethics and responsibilities)

6. a. Gram staining of bacteria.

b. Measurement of size of cells by Micrometry.



7. a. Enumeration of total count. (haemocytometer)

b. Motility of Bacteria by hanging drop technique.

(PO1-M Knowledge transfer: Knowledge of basic techniques in

microbiology, PO5-L Modern tool usage)

8. Growth curve of bacteria by turbidometry.

9. Effect of temperature and PH on growth of bacteria.

10. Biochemical test: Catalase test, Starch Hydrolysis, Gelatin

liquefaction,

proteolytic activity.

11. Biochemical test: Carbohydrate fermentation test and IMViC Test.

12. Antibiotic susceptibility testing of bacteria and antagonism test

between fungi and bacteria.

13. Lysis of bacteria by phage, plaque counting.

14. Measurement of fungal growth by linear determination.

(P01-H: Knowledge transfer: Knowledge of basic science, PO9-L

Individual and team work).

15. Bacteriological examination of water by multiple tube fermentation

tests.

(P01-H: Knowledge transfer: Knowledge of basic science, PO6-M

knowledge to assess societal and health issues).

TEXT BOOKS:

1 Cappuccino J G

and Sherman

Microbiology, A Laboratory Manual, Benjamin /

Cummings Publishing Company Inc, 10th

Edition, 1996, 978-1355992486

2 Benson H J. Microbiological Applications- (A Laboratory

manual in General Microbiology), McGraw Hill,

Boston, 8th Edition, 2002, 978-1259919794

3 Aneja R K. Experiments in Microbiology, Plant Pathology, &

Biotechnology, New Age International Publisher,

5th Edition, 1957, 978-0967377360



Course Outcomes: After the completion of this course, students will be able

to:

1. Describe and enumerate practical skills for isolation of different types

of microorganisms and identify them. Preparation of different types of

media in broth and solid form. (BL: L1, L2) (PO1-L, PO4-M, PO6-L,

PO8-M, PSO3-L)

2. Demonstrate the procedures pertaining staining of microbes,

identification and its movement. (BL: L1, L2) (PO1-M, PO5-L, PSO1-M,

PSO3-M)

3. Apply analytical equipment for enumeration of total count of microbes

from different samples and calculate the load of microorganisms

present in different samples. (BL: L1, L2) (PO1-H, PO5-L, PSO3)

4. Formulate media for production of bioactive molecules based on the

nature of growth of microorganism. (BL: L2, L3) (PO1-H, PO9-L, PSO1-

M, PSO3-M)

5. Interpret and analyse the potability of drinking water, food samples

from different sources etc. (BL: L2, L3) (PO1-H, PO6-M, PSO1-M, PSO3-

M)



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBTL01 2 1 1 1 1 1 1 1




Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Describe and enumerate practical skills for isolation of different types of microorganisms and identify them. Preparation of different types of media in broth and solid form.

1 2 1 2

l l

Demonstrate the procedures pertaining staining of microbes, identification and its movement

2 1

Apply analytical equipments for enumeration of total count of microbes from different samples and calculate the load of microorganisms present in different samples.

3 1

Formulate media for production of bioactive molecules based on the nature of growth of microorganism

3 1

Interpret and analyze the potability of drinking water, food samples from different sources etc

3 2




BIOCHEMISTRY LABORATORY

Lab Hours/

Week

: 0+0+3 (L+T+P) Credits: 1.5

Course Code : 3RBTL02 CIE Marks: 50

SEE Marks: 50

Course Objectives: This course will enable students to: 1. Understand fundamental approaches in preparing various

solutions of different concentration.

2. Enhance the practical approaches in estimation of biomolecules

like carbohydrates, proteins and lipids.

3. Apply the concepts of biochemical methods of investigating samples

in realistic situations.

4. Apply the biochemical methods to understand the properties of

phenolic compounds.

5. Apply the biochemical methods to understand the properties of

hydrogen peroxide.

List of experiments:

Demonstration: Calibration of pH meter, Measurement of pH of the

solution, spectrophotometer, concentration of solutions (Normality,

Molarity and Molality), preparation of buffers of constant strength.

1) Titration curve of amino acids.

2) Qualitative tests for carbohydrates.

3) Qualitative tests for proteins.

4) Qualitative tests for lipids.

(P01-L: Knowledge transfer: Knowledge of basic science PO2-L: To

gather knowledge on biomolecules and applying the knowledge in

identifying the type of biomolecule in unknown solution).

5) Estimation of blood sugar by O-Toluidine method.

6) Estimation of blood sugar by Folin-Wu method.

7) Determination of total carbohydrate by anthrone method.




gather knowledge on carbohydrates and applying the knowledge in

identifying the carbohydrates by various methods).

8) Estimation of inorganic phosphate by Fiske-Subbarao method.

9) Estimation of protein by Bradford’s method and Lowry’s method.

10) Determination of iodine value of lipid.


gather knowledge and use of appropriate method to differentiate).

11) Determination of saponification value of lipid.

12) Estimation of urea by diacetylmonoxime method.

13) Estimation of iron from haemoglobin.


gather knowledge and use of appropriate method to differentiate).

14) Estimation of total phenolic compounds.

15) Estimation of H2O2 using silver nano particles.

(P01-M: Knowledge transfer: Knowledge of basic science).

TEXT BOOKS:

1 David T Plummer Introduction to Practical Biochemistry, McGraw

Hill Education, 3rd Edition, 2017,

9780070994874

2 Sadashivam, S.

Manikkam, A.

An Introduction to Biochemical Methods, New Age

International Publishers, 3rd Edition, 2007, 978-

0121825546

REFERENCE BOOK:

1 Pattabhiraman,

T.N.

Lab manual in Biochemistry, All India Pub & Dist,

4th Edition, 2007, 978-1305968554


able to:

1. Classify and differentiate the biological molecules like proteins,

carbohydrates and proteins by quantitative estimation. (BL: L2, L3)

(PO1-L, PO2-L, PSO1-M, PSO3-L)



2. Identify and interpret the sugar content in the solution by various

methods. (BL: L2, L3)(PO1-L, PO2-L, PSO1-M, PSO3-L)

3. Describe and interpret the methods of protein, iodine and inorganic

phosphate estimation. (BL: L2, L3)(PO1-L, PO2-L, PSO1-M, PSO3-L)

4. Analyze and interpret the methods of lipids, iron and urea

estimation. (BL: L2, L3)(PO1-L, PO2-L, PSO1-M, PSO3-L)

5. Explain the methods of phenolic and hydrogen peroxide estimation.

(BL: L2) (PO1-M, PSO1-M, PSO3-L)


OUTCOMES


Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

3RBTL02 1 1 2 1


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Classify and differentiate the biological molecules like proteins, carbohydrates and proteins by quantitative estimation.

1 1 2 1

Identify and interpret the sugar content in the solution by various methods.

1 1 2 1

Describe and interpret the methods of

protein, iodine and inorganic phosphate

estimation.

1 1 2 1

Analyze and interpret the methods of

lipids, iron and urea estimation 1 1 2 1

Explain the methods of phenolic and hydrogen peroxide estimation.

2 2 1




CONSTITUTION OF INDIA AND PROFESSIONAL ETHICS



Course Code : HSS04 SEE Marks : 50

Course outcomes: This course will enable students to:

1. To orient students on the salient features of the Indian Constitution with special emphasis on fundamental rights and duties.

2. To provide an overview of the Union and State legislature, executive and judiciary.

3. To explain the importance of electoral process and help students appreciate the ethical practices in elections.

4. To facilitate the students to have a clear perspective of the Ethical values and their relevance in the present context of globalization.

5. To enable the students familiarize themselves with the ethical values proposed and practiced by the great leaders / eminent personalities with particular reference to Sir M. Visvesvaraya.

Part I: Constitution of India

Unit I

Introduction to Constitution of India: Salient Features of Indian

Constitution, Preamble to the Indian Constitution, Different kinds of fundamental rights, Directive Principles of State Policy, Categorical study of Directive Principles, Relationship between DPSP and Fundamental Rights, Fundamental Duties. 6 Hrs

Unit II

Union and State Governments: Union and State Legislature:

Composition and powers of Loksabha, Rajyasabha, Legislative Assembly and Legislative Council. Union and State Executive: The appointment and powers of President, Vice-president, Prime-Minister, Union Council of Ministers, Governor of State, Chief Minister of State and State Council of Ministers. Union and State Judiciary: The Composition and powers of

Supreme Court and High Court. 7 Hrs

Unit III

Other Provisions of Constitution: Special provisions relating to SC / ST, Women and Children and other backward classes. Electoral process related to the Election Commission of India: Composition, functioning and removal of the Election Commissioner(s).



Human Rights: Meaning, Kinds and Safeguard of Human Rights, State

Human Rights Commission and National Human Rights Commission. 5 Hrs

Part II -Professional Ethics

Unit IV

Professional Ethics and Human Values: Introduction, What is Ethics, Scope of Professional Ethics, Values and Characteristics, Types of values: Negative and positive values, Ethical values for Professional success. Case Studies: Ethical practices of the reputed Indian Companies: TATA Group, Wipro Technologies. 4 Hrs

Unit V

Professional Code of Conduct: Introduction, Professional Code, Legal System, Ethical and Unethical practices, Making the Professional Code successful. Ethical values of Sir M. Visvesvaraya, Mahatma Gandhi and Swami Vivekananda. 4 Hrs

TEXT BOOKS:

J N Pandey Constitutional Law of India, Central Law Agency

Publishing, 49th Edition, 2016, 978-9384852412

S G Hunderker (Ed.) Business Ethics and Human Values, Excel Books,

New Delhi, 48th Edition, 2009, 978-8174467386

REFERENCE BOOKS:

M.V. Pylee Constitution of India, Vikas Publishing House,

New Delhi, 5th Edition, 2014, 978-8125913757

D.D. Basu Shorter Constitution of India, Lexis Nexis, 2nd

Edition, 2015, 978-9357434467

D.D. Basu Commentary on the Indian Constitution (Vol. 10),

Butter worth’s, Wadhwa Nagar, Nagpur, 2016, 978-9350356661

OC Fcrrell, John

Paul Fraedrich,

Linda Ferrell

Business Ethics: Ethical Decision Making and

Cases, Biztantra, New Delhi, 2014, 978-

1285423715

Swami Vivekananda My India: The India Eternal – Ramakrishna

Mission Institute of Culture, Kolkata, 1993, 978-

9350356661



IV Semester Syllabus

2020-21



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MOLECULAR BIOLOGY





1. Study the principles of central dogma of molecular biology and

molecular nature of chromosome. To understand the concept of gene

replication.

2. Study the transcription mechanisms in prokaryotes and eukaryotes.

3. Study the translation mechanisms in prokaryotes and eukaryotes.

4. Study gene regulation and expression in prokaryotes and

bacteriophages.

5. Study the genetic recombination and gene mapping.

Unit I

Introduction: Chromosomal theory of heredity - by W S Sutton and

T.Boveri, Information flow in biological systems: Central dogma and its

reversal, RNA self-replication, updated central dogma (Retroviruses).

Replication:

Introduction, Replication of DNA - General features, semi-conservative

DNA replication in E.coli, mechanism of DNA replication, Meselson and

Stahl’s experiment. Mechanism of DNA replication in Eukaryotes -

Eukaryotic DNA replication, multiple replication forks, initiation,

elongation & termination, Replication of chromatin. Replication of

telomeres, Models of replication in – Rolling circle model, Cairn’s model

and Yoshikawa model. Enzymes of DNA replication, Differences in

prokaryotic and eukaryotic DNA replication.

DNA damage and repair: Introduction& types of damage; DNA repair –

introduction, types- Photo reactivation, mismatch repair, excision repair,

recombination repair & SOS repair and recombination, mutagenic

repairs system, genetic control of DNA repair. (P01-L: Knowledge

transfer: Knowledge of basic science)

12 Hrs



Unit II

Transcription: Introduction. Concept of sense and antisense strands.

Structure and function of bacterial RNA polymerase, sigma factor cycle.

Transcription in Prokaryotes - initiation, elongation, termination (rho

dependent and rho independent) Transcription in Eukaryotes.

Eukaryotic RNA Polymerases-I, II and III functions & their promoters.

Steps in transcription-initiation, elongation & termination (Pol II only),

Difference in prokaryotic and eukaryotic transcription, Transcription

factors, Post-transcriptional modification of mRNA-capping,

polyadenylation, splicing- excision of introns &splicing of exons (All three

methods). Alternative splicing. Transcription inhibitors. (P01-L:


10 Hrs

Unit III

Translation: Introduction, definition, steps-activation of amino acid,

Initiation, elongation & termination (Prokaryotes) Protein synthesis in

eukaryotes - Initiation, elongation and termination Differences in pro- &

eukaryotic protein synthesis. Protein targeting and structure of Gene: Protein targeting, Protein

splicing, post-translational modification of proteins (acetylation,

phosphorylation) Inhibitors of translation. (P01-L: Knowledge transfer:

Knowledge of basic science)

10 Hrs

Unit IV

Concept of gene regulation:

Gene regulation and expression in prokaryotes and bacteriophages; types

in prokaryotes & eukaryotes; positive versus negative regulation.

Induction and repression system.

Fine structure of Gene: Structure and regulation of gene expression,

Operon model – Lac Operon- positive regulation and negative regulation,

mutations in lac operon, Trp-Operon - by an allosteric repressor protein

and by an attenuator and Gal operon. (P01-M: Knowledge transfer:

Knowledge of basic science) 10 Hrs

Unit V

Genetic recombination and gene mapping: Genetic recombination in

bacteria and viruses – transformation, conjugation, transduction



(Generalized and specialized), transposons and insertion sequences; Role

of recombination and transposition in evolution; gene mapping techniques. (P01-L: Knowledge transfer: Knowledge of basic science)

10 Hrs

TEXT BOOKS:

1 Freifelder Molecular Biology, Narosa Publications, 2 Edition,

2005, 978-9350356675

2 David

Freifelder

Freifelder’s Essentials of Molecular Biology, Narosa

Publications, 4th Edition, 2009, 978-9350346624

REFERENCE BOOKS:

1 Darnell J Lodish & H

Balitimore.

Molecular cell Biology, Scientific American

books, USA, 4th Edition, 2015, 923-

9352546322

2 Gardener,Simmons,

Snustad.

Principles of Genetics, Wiley publishers,

8th Edition, 2005, 932-9352366314

3 Benjamin Lewin Genes IV, Oxford university press, 4th

revised Edition,1990, 912-8952366323


1. Explain the process of DNA replication, damage and repair

mechanism in prokaryotes and eukaryotes. (BL: L1) (PO1-L, PSO1-

L)

2. Describe the process of transcription in prokaryotes & eukaryotes.

(BL: L1,) (PO1-L)

3. Discuss the process of translation in prokaryotes & eukaryotes.

(BL: L1) (PO1-L)

4. Explain the concepts of gene regulation & expression. (BL: L2)

(PO1-L)

5. Illustrate the process of genetic recombination and gene mapping.

(BL: L1, L2) (PO1-L)





Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBT01 1 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Explain the process of DNA replication, damage and repair mechanism in prokaryotes and eukaryotes

1 2

Describe the process of transcription in prokaryotes & eukaryotes

1 2

Discuss the process of translation in

prokaryotes & eukaryotes 1 2

Explain the concepts of gene regulation & expression

2 2

Illustrate the process of genetic recombination and gene mapping

1 2




GENETIC ENGINEERING




Course objectives: This course will enable students to: 1. Study rDNA technology, vectors and enzymes used in genetic

engineering.

2. Acquire the knowledge of specific techniques like PCR, NA

hybridization & libraries.

3. Learn about various gene transfer techniques, applications of

transgenic plants &animals and importance of gene therapy.

4. Study the various application of genetic engineering in health care

and environment.

5. Understand the important of Genetically Modified Organisms

(GMOs).

Unit I

Vectors & Enzymes in Genetic Engineering: Vectors in rDNA

technology: salient features of vectors, types of vectors. Plasmids:

Definition and classification of plasmids, Natural plasmid (ColE1) and

artificial plasmid (pBR322, PUC series), Phage vectors: ssDNA vectors

(M13) Viruses, Cosmids and Artificial Vectors (BACs, YACs). (PO1- M :

Engineering knowledge) Enzymes in Genetic Engineering:

Exonucleases and Endonucleases: classification, mode of action.

Enzymes in modification -Polynucleotide phosphorylase, DNase,

Methylases, phosphatases, polynucleotide Kinase, Ligases, RNase and

their mechanism of action.DNA of Cell Organelle. (PO1-M: Engineering

knowledge) 12 Hrs

Unit II

Nucleic acid isolation, amplification and sequencing: Isolation and

purification of nucleic acids: (DNA, RNA and plasmids), quantification

and Storage. (PO2: M: solutions based on fundamental principles)

Introduction to Primer and Probe: Definition, Preparation Probes, and its

application. Methods of nucleic acid detection: polymerase chain



reaction (PCR), variants of PCR (RT-PCR and Q-PCR) and applications.

(PO1-M: Engineering knowledge) DNA Sequencing: Definition of

Sequencing Concept, Methods: Maxam and Gilbert method and Sanger,

Automated DNA sequencing. Advantages and disadvantages. Recent

trends in DNA Sequencing. (PO2: M: solutions based on fundamental

principles). 10 Hrs

Unit III

Nucleic acid hybridization and Methods of Gene/DNA transfer:

Methods of nucleic acid hybridization: Southern, Northern & Western

hybridization techniques & applications. (PO2: M: solutions based on

fundamental principles) Gene transfer techniques: Biological

(bactofection and transduction), Chemical (Calcium phosphate, DEAE

dextran, Cationic Lipid) and mechanical (Electroporation, Microinjection,

gene gun, Sonoporation, Laser induced and Bead transfection). (PO5-L:

Modern tool usage), (PO8-M: Apply ethical principles) Agrobacterium-

mediated gene transfer in plants – Ti & RI plasmids: structure and

functions, Ti plasmid based vectors - advantages, disease control of

Agrobacterium tumefaciens. Chloroplast transformation & its

applications (PO1-M : Engineering knowledge)

10 Hrs

Unit IV

Transgenic science in genetic improvement: Transgenic science in

plant improvement, biopharming - plants as bioreactors, transgenic

crops for increased yield, resistance to biotic and abiotic stresses.

Techniques of gene mapping in plants. Marker-assisted selection and

breeding for improvement. Transgenic science for animal improvement,

biopharming -animals as bioreactors for recombinant proteins, Gene

mapping in farm animals. Marker-assisted selection and genetic

improvement of livestock. (PO2: M: solutions based on fundamental

principles) (PO8-M: Apply ethical principles)

10 Hrs

Unit V

Applications and advance genome editing: Microbial biotechnology –

Genetic manipulation, Methods: engineering microbes for the production

of antibiotics, enzymes, Insulin, growth hormones, clearing oil spills.

Gene Therapy: Introduction, methods of Gene therapy (Ex Vivo and



Invivo), Genome editing- (Zinc finger nucleases (ZFNs), CRISPR

technology: Recent trends in CRISPR. (PO2: M: solutions based on

fundamental principles) (PO5-L: Modern tool usage) (PO8-M: Apply

ethical principles)

10 Hrs

TEXT BOOKS:

1 Desmond S. T. Nicholl An Introduction to Genetic

Engineering, University Press, 3rd

Edition, 2008, 978-0521615211

2 T.A. Brown Gene Cloning and DNA Analysis - An

Introduction, Wiley-Blackwell Science,

6th Edition, 2010, 978-1119072560

REFERENCE BOOKS:

1 Glick, B R, Pasternak J.

J

Molecular Biotechnology: Principles

and Applications of Recombinant DNA

by, DC ASM Press, 3rd Edition, 2003,

978-1555814984

2 Brown T. A. Gene Cloning & DNA Analysis: An

Introduction, Blackwell Science, 5th

Edition, 2006, 978-1119072560


1. Classify and compare the various types of vector and enzymes in

Genetic Engineering. (BL: L2) (PO1-M)

2. Identify and differentiate the techniques to isolation, amplify and

sequencing. (BL: L2) (PO1-M, PO2-M)

3. Distinguish various hybridization techniques and gene transfer

methods. (BL: L3) (PO1-M, PO2-M, PO5-L, PO8-M)

4. Outline the various methods of producing transgenic organisms in

genetic improvement. (BL: L3) (PO2-M, PO8-M)

5. Describe the applications of genetic engineering for the welfare of

mankind & society. (BL: L2) (PO2-M, PO5-L, PO8-M)





Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBT02 2 2 1 2 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Classify and compare the various types of vector and enzymes in Genetic Engineering.

2 2

Identify and differentiate the techniques to isolation, amplify and sequencing.

2 2 2

Distinguish various hybridization techniques and gene transfer methods.

2 2 1 2 2

Outline the various methods of producing transgenic organisms in genetic improvement.

2 2 2

Describe the applications of genetic engineering for the welfare of mankind & society.

2 1 2 2




BIOSTATISTICS AND BIOMODELING

Contact Hours/Week : 3+1+0 (L+T+P) Credits : 3.5


Total Tutorial Hours : 13 SEE Marks : 50

Course Code : 4RBT03


1. Study the scope of Annova.

2. Learn the laws & principles of probability distribution.

3. Understand the significance of statistical inference in biological

experiments.

4. Learn the design of experiments.

5. Study the biomodelling of microbial growth in a chemostat, growth

equations of microbial populations, models for inheritance.

Unit I

Analysis of variance: Introduction, Assumption in the Analysis of

variance, Technique of Analysis of variance, the basic Principal of

ANOVA, Analysis of variance is One-way classification, Analysis of

variance is Two-way classification.

(PO1-M: Knowledge transfer to solve simple engineering problems)

8+3 Hrs

Unit II

Bi-variate distribution: Correlation and regression analysis (simple and

linear) curve fitting (linear, non-linear and exponential). Standard

deviation and standard error concepts and problems.


Probability

Axioms, models, conditional probability, Bayes rule, Genetic Applications

of Probability, Hardy - Weinberg law, Wahlund's Principle, Forensic

probability determination, Likelihood of paternity, Estimation of

probabilities for multilocus/ multi-allele finger print systems. P- value

and Q-value.


8+3 Hrs



Unit III

Statistical inference: Estimation theory and testing of hypothesis, point

estimation, interval estimation, sample size determination, simultaneous

confidence intervals, parametric and non-parametric distributions (T-

test, F-test, Chi Squared distribution, goodness of fit test) analysis of

variance (one-way and two-way classifications). Case studies of statistical

designs of biological experiments (RCBD, RBD).


9+2 Hrs

Unit IV

Probability distributions: Discrete probability distributions - Binomial,

Poisson, geometric – derivations. Central limit theorem. Continuous

probability distribution – normal, exponential, gamma distributions, beta

and Weibull distributions, T & F distributions.

Design of experiments:

Sample surveys, comparisons groups and randomization, random

assignments, single- and double-blind experiments, blocking and

extraneous variables, limitations of experiments.


7+2 Hrs

Unit V

Case studies: Cigarette smoking, Lung cancer, endangered plants

species, epidemics.

Biomodeling:

Microbial Growth in a Chemo stat, Growth Equations of Microbial

populations, Models of Commensalisms, Mutualism, Predation and

Mutation. Volterra’s Model for n Interacting Species. Basic Models for

Inheritance, Selection and Mutation Models, Genetic Inbreeding Models.

(PO2-M: Identifies all relevant constraints and requirements and

formulates ac accurate description of the problem)

7+3 Hrs



TEXT BOOKS:

Marcello Pagano &

Kimberlee Gauvreu

“Principles of Biostatistics”, Thompson

Learning, 2nd Edition, 2000, 978-1451130171

Ronald N Forthofer

and Eun Sul Lee

“Introduction to Biostatistics”, Academic Press,

1st Edition, 1996, 965-1251130174

Norman T J Bailey “Statistical methods in Biology”, Cambridge

University Press, 3rd Edition, 1995, 934-

6551130578

J.N.Kapur “Mathematical Models in Biology and Medicine”,

Oscar Publication, 3rd Edition 2010, 978-

8185336824

H Bancroft, J. Ispen

and P Feigl

“Introduction to Biostatistics”, Harper & Row,

New York,2nd Edition, 1970, 932-1551130556

Dutta, Animesh, K “Basic Biostatistics & its Applications”, New

Central Book Agency, 1st Edition, 2007, 956-

1951130578

REFERENCE BOOKS:

S I Rubinow “Introduction to Mathematical Biology”, Wiley

Publishers, 1st Edition, 1975, 8480522565

P.S.S. Sundar Rao

and J.Richard

“An Introduction to Biostatistics”, Prentice Hall of

India Pvt Ltd, 5th Edition, 2006, 8280522556

Miller, Freund’s and

Richard A Johnson

“Probability and Statistics for Engineers”, Chegg

Publishers, 8th Edition, 2011, 8580522578



Veer Bala Rastogi “Fundamentals of Biostatistics”, ANE Books

India, 2009, 3rd Edition, 8180522555


1. Explain the concepts of Annova. (BL:L2) (PO1-M)

2. Describe different laws of probability. (BL:L2) (PO1-M)

3. Apply concepts of analysis of variance in inferring the statistical

data. (BL:L3) (PO1-M)

4. Apply different methods in design of experiments. (BL:L3) (PO1-M)

(PO1-M)

5. Outline the case studies of lung cancer, endangered plants species.

(L1) (PO2-M)


OUTCOMES


Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBT03 2 2 2 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Explain the concepts of Annova 2 2

Describe different laws of probability 2 1 1

Apply concepts of analysis of variance in

inferring the statistical data 2 1 1

Apply different methods in design of experiments

2 2 2

Outline the case studies of lung cancer, endangered plants species

2 2




BIOLOGICAL THERMODYNAMICS Contact Hours/Week : 3+1+0 (L+T+P) Credits : 3.5


Total Tutorial Hours : 13 SEE Marks : 50

Course Code : 4RBT04


1. Study Measurement of various types of energies and enthalpy.

2. Learn entropies of second law and third law of thermodynamics.

3. Understand the variation of Gibbs free energy and stability of

nucleic acids and proteins.

4. Know response of equilibria in presence of catalyst and

temperature.

5. Study the thermodynamics of ion and electron transport.

Unit I

The First Law: Systems and surroundings, work and heat, measurement of work, measurement of heat, internal energy, enthalpy, enthalpy changes accompanying physical processes, bond enthalpy,

thermochemical properties of fuels, combination of reaction enthalpies, standard enthalpies of formation, enthalpies of formation and computational chemistry, variation of reaction enthalpy with temperature. (PO1-M, Knowledge of mathematics & Engineering

fundamentals) 8+3 Hrs

Unit II

The Second Law: The direction of spontaneous change, entropy and the second law, absolute entropies and the third law of thermodynamics, entropy changes accompanying chemical reactions, Gibbs free energy,

hydrophobic interaction, work and the Gibbs free energy change. (PO1-M,

Knowledge of mathematics & Engineering fundamentals) 7+3 Hrs

Unit III

Phase Equilibria: The condition of stability, the variation of Gibbs free energy with pressure, the variation of Gibbs free energy with temperature, phase diagrams, the stability of nucleic acids and proteins,



phase transitions of biological membranes, the chemical potential, ideal

and ideal–dilute solutions, the modification of boiling and freezing points, osmosis. (PO1-M, Knowledge of mathematics, Sciences &

Engineering fundamentals)

8+3 Hrs

Unit IV

Chemical Equilibrium: The reaction Gibbs free energy, the variation of

reaction Gibbs free energy with composition, reactions at equilibrium,

the standard reaction Gibbs free energy, the response of equilibria to the

presence of a catalyst, the effect of temperature on equilibria, Brønsted–

Lowry theory, protonation and deprotonation, polyprotic acids,

amphiprotic systems, buffer solutions. (PO1-M, Knowledge of

mathematics, Sciences & Engineering fundamentals)

8+2 Hrs

Unit V

Thermodynamics of Ion and Electron Transport: Ions in solution,

passive and active transport of ions across biological membranes, ion

channels and ion pumps, half-reactions, reactions in electrochemical

cells, the Nernst equation, standard potentials, applications of standard

potentials - the determination of thermodynamic functions, the

electrochemical series, the respiratory chain, and plant photosynthesis.

(PO1-M, Knowledge of mathematics, Sciences & Engineering

fundamentals)

8+2 Hrs

TEXT BOOKS:

Atkins P. and de

Paula J. Physical Chemistry of the Life Sciences, W.H

freeman and Company, New York, 2nd Edition,

2011, 978-1-4292-3114-5

REFERENCE BOOKS:

Hammes G.G and

Hammes-Schiffer S. Physical Chemistry for the Biological Sciences,

John Wiley & Sons, Inc., Hoboken, New

Jersey, 2 Edition, 2015, 978-1-118-85900-1



Allen J.P. Biophysical Chemistry, Wiley-Blackwell,

Chichester, West Sussex, UK, 3 Edition, 2008,

978-1-4051-2436-2


1. Discuss the various types of enthalpy and energy accountable in

First law of Thermodynamics and its measurement. (BL: L2) (PO1-

M)

2. Calculate the entropy changes and Gibbs free energy in chemical

reactions. (BL: L3) (PO1-M)

3. Determine the stability of nucleic acids and proteins, chemical

potential of biological systems. Explain conditions of stability and

the modification of boiling and freezing points & osmosis. (BL: L2,

L4) (PO1-M)

4. Explain the effect of temperature on equilibria and Bronsted–Lowry

theory. And also Evaluate the standard Gibbs free energy for

reactions. (BL: L5, L6) (PO1-M)

5. Discuss the Ions transport in solution, across biological

membranes, ion channels and ion pumps. And also determine

standard potentials thermodynamic functions. (BL: L2, L4) (PO1-M)




OUTCOMES


Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBT04 2 2 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Discuss the various types of enthalpy and energy accountable in First law of Thermodynamics and its measurement

2 2

Calculate the entropy changes and Gibbs free energy in chemical reactions

2 2

Determine the stability of nucleic acids and proteins, chemical potential of biological systems. Explain conditions of stability and the modification of boiling and freezing points & osmosis

2 2

Explain the effect of temperature on equilibria and Brønsted–Lowry theory. And also Evaluate the standard Gibbs free energy for reactions

2 2

Discuss the Ions transport in solution, across biological membranes, ion channels and ion pumps. And also

determine standard potentials thermodynamic functions

2 2 3




INSTRUMENTAL METHODS OF ANALYSIS



Course Code : 4RCCB1 SEE Marks : 50

Course Objectives: This course will enable students to: 1. Understand the basic concepts, principles of chromatographic

separations and operation of modern chromatographic

instrumentation.

2. Understand the basic principles of electronic spectroscopic

techniques and explain the terminology of UV/Vis spectroscopies.

Examine UV spectra based on the knowledge of different electron

transitions.

3. Know the theory of IR absorption, types of vibrations, factors

affecting the group frequencies and sample handling techniques. To

analyze IR spectra based on knowledge of characteristic functional

group frequencies.

4. Demonstrate the knowledge of the chemical shifts and coupling

constants in NMR to study 1H NMR spectra and propose structures

for compounds.

5. Understand about characterization techniques such as XRD and

electron microscopy which interpret crystal structure and

morphology.

Unit I

Chromatography: Introduction to Chromatography - Classification -

Theory - terminologies- distribution coefficient, retention time, corrected

retention time, retention volume, corrected retention volume, retention

factor, selectivity factor, column capacity, separation number, peak

capacity, column efficiency, resolution and optimization of column

performance. Types of chromatography- adsorption, partition, ion

exchange and size exclusion chromatography. Numerical problems on

retention factor.

Thin layer chromatography: Principle, mobile phase, sample

application, development techniques evaluation and documentation,

advantages, limitations and applications.



Gas chromatography:

Principle, instrumentation, carrier gas, stationary phase, sample

injection, columns, detectors (TCD, FID, ECD atomic emission detector).

Applications.

High performance liquid chromatography:

Principle, instrumentation, column, sample injection, detectors (UV,

refractive index), mobile phase selection, isocratic and solvent gradient

system. Demonstration of HPLC, Applications. (PO1-L Transfer of Basic

knowledge in Chemistry, PO4-M, Calibration and Data Analysis)

8 Hrs

Unit II

General introduction to spectroscopy: Introduction, Types of

spectroscopy-atomic and molecular spectroscopy, nature and interaction

of electromagnetic radiations with matter, energies corresponding to

various kinds of radiations, spectral band width – definition and factors

contributing spectral width, factors influencing positions and intensity of

spectral lines. (PO1-L Transfer of Basic knowledge in Chemistry)

4 Hrs

Electronic Spectroscopy: Principles of electronic spectroscopy - Types

of electronic transitions in organic molecules. Chromophores and

auxochromes. Bathochromic shift or Red shift, hypsochromic shift or

blue shift. Hyperchromic effect and hypochromic effect. Effect of solvent

and extent of conjugation on max and on the energies of n −* and −*

transitions. Instrumentation, qualitative and quantitative analysis.

(PO1-L Transfer of Basic knowledge in Chemistry)

4 Hrs

Unit III

Infrared spectroscopy: Principles of IR spectroscopy. Requirements for

IR absorption. Types of vibrations - Stretching vibrations and bending

vibrations. Fundamental modes of vibrations for linear and non linear

molecules. Characteristic group frequencies for infrared absorption of

organic molecules. Factors affecting the group frequencies – coupled

interactions (Fermi resonance, aldehyde) electronic effects (carbonyl

compounds) and hydrogen bonding (alcohols, carboxylic acids).

Numerical problems on vibrational frequencies. Instrumentation-FTIR



instrument and its advantages. Sample handling techniques – Nujol mull

and KBr pellet. (PO1-L Transfer of Basic knowledge in ChemistryPO4-

M, Data Analysis)

8 Hrs

Unit IV

Nuclear magnetic resonance spectroscopy: The nuclear spin, Larmor

precession, the NMR isotopes, energy levels for a nucleus with spin

quantum number I = ½ , 3/2 and 5/2, theory of population of nuclear spin

levels, spin-spin and spin-lattice relaxation, chemical shift – definition,

causes, measurement. TMS as a reference compound and its advantages,

factors affecting chemical shift, shielding and deshielding mechanisms,

correlation of chemical shifts with chemical environment – aliphatic,

alkenic, alkynic, aldehydic, ketonic, aromatic, alcoholic, phenolic,

carboxylic, amino protons, spin – spin coupling, spin – spin splitting,

intensity ratio of multiplet- Pascal’s triangle method, chemical exchange,

effect of deuteration, classification of spin systems (AX, AMX, AB, ABC),

first order spectra, low and high resolution spectra, determination of

peak areas, coupling constants-short and long range couplings,

Instrumentation – FT NMR. Applications of electronic spectroscopy, IR

and NMR to structural elucidation of simple organic molecules. (PO1-L

Transfer of Basic knowledge in Chemistry)

8 Hrs

Unit V

Microstructures and morphological studies: XRD: Production of X-

rays; types of X-ray sources, Selection of radiation, Braggs Equation,

Diffraction by Crystal - direction and intensity of diffracted beams,

Calculation of particle size-Debye Scherrer equation proportional,

scintillation, solid-state detectors. X-ray spectroscopy for elemental

analyses - wavelength dispersive and energy dispersive analyses. AFM –

principle and applications, Demonstration and Data analysis. (PO1-L

Transfer of Basic knowledge in Chemistry)

Microscopy:

Concept of optical microscopy, uses, advantages and disadvantages,

Electron microscopy, Introduction, Theory of electron diffraction,

Scanning electron microscopy (SEM), Demonstration and Data analysis

(PO4-M, Calibration and Data Analysis )



Transmission electron microscopy (TEM), indexing selected area electron

diffraction pattern, HRTEM analysis, Comparison of XRD and TEM

(HRTEM, SAED pattern). (PO4-M, Data Analysis) AFM – principle and

applications. 7 Hrs

TEXT BOOKS:

R.M. Silverstein and

W.P. Webster Spectrometric Identification of organic

compounds, Wiley & Sons, 8th Edition, 1999,

978-0470616376

H H Willard, L.L.

Merritt and J.A.

Dean and F.A. Settle

Instrumental Methods of Analysis, CBS

Publishers, 7th Edition, 1988, 945-0470612323

REFERENCE BOOKS:

G.W. Ewing Instrumental methods of Chemical Analysis,

McGraw-Hill, New York, 5th Edition, 1988, 912-

0470612345

Skoog, D.A, S.J.

Holler, T.A.

Nilman,

Principles of Instrumental Analysis, Saunders

college publishing, London,5th Edition, 1998, 978-

0270612378

Jaffery, Gill,

Basset. J

Vogel’s Text Book of Quantitative Inorganic analysis,

ELBS, 5th Edition, 1998, 956-0450612323


1. Describe the chromatographic techniques for the identification and

purification of compounds. (BL: L2) (PO1-L, PO4-M)

2. Explain the principles and methods of different spectroscopic

techniques. (BL: L2) (PO1-L)

3. Discuss the theory of IR absorption, types of vibrations and analyse

IR spectra. (BL: L2) (PO1-L, PO4-M)



4. Interpret and perform NMR spectra analysis for the identification of

unknown organic molecules. (BL: L2) (PO1-L)

5. Explain the characterization techniques: XRD and electron

microscopy which interpret crystal structure and morphology. (BL:

L2) (PO1-L, PO4-M)



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RCCB1 1 2 2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Describe the chromatographic techniques for the identification and purification of compounds

1 2 2

Explain the principles and methods of different spectroscopic techniques

1 2

Discuss the theory of IR absorption, types of vibrations and analyse IR spectra

1 2 2

Interpret and perform NMR spectra analysis for the identification of unknown organic molecules.

1 2

Explain the characterization techniques: XRD and electron microscopy which interpret crystal

structure and morphology.

1 2

2




TRANSPORT PROCESSES - II Contact Hours/Week : 3+0+0 (L+T+P) Credits : 3




1. Understand the basic concepts of conduction, and critical

thickness of insulation.

2. Learn the concepts of LMTD, Dimensionless numbers and detailed

construction & types of heat exchangers.

3. Study the fundamentals of mass transfer and various types of

diffusion processes.

4. Study the principles and different types of distillation.

5. Understand the concepts of extraction and drying along with

different types of industrial equipment.

Unit I

Heat Transfer:

Introduction: Various modes of heat transfer, Conduction, Convection,

Radiation. Conduction: Basic law of conduction, Fourier’s law, Thermal

conductivity, Steady state unidirectional heat flow through single and

multiple layer slabs, cylinders and spheres with constant thermal

conductivities. Insulation: Introduction and types of insulation, critical

thickness of insulation. (PO1: M: Apply the knowledge of

mathematics, science, engineering fundamentals)

7 Hrs

Unit II

Convection, radiation and heat exchange equipment: Heat transfer by

natural and forced convection. Individual and overall heat transfer co-

efficient (HTC), LMTD, LMTD correction factor. Estimation of HTC under

natural and forced convection using correlations. Principles of radiation

and microwave heating. HT devices: double pipe heat exchanger, shell

and tube heat exchanger, Plate heat exchanger and single effect

evaporator. Dimensionless numbers in heat transfer and their

significance. (PO2: H: Identify and analyze complex engineering

problems reaching substantiated conclusions using first principles

of mathematics) 8 Hrs



Unit III

Mass transfer: Introduction to mass transfer operations, classification

of mass transfer operations and their application in industries. Molecular

diffusion: Fick’s law of diffusion and its application for steady state

diffusion of component A through non-diffusing B. Equimolar counter

current diffusion. Mass transfer coefficient and its correlations.

Numerical problems. Molecular diffusion in biological solutions and gels.

Mass transfer theory: Film theory and its significance. (PO2: H: Identify

and analyze complex engineering problems reaching substantiated

conclusions using first principles of mathematics)

8 Hrs

Unit IV

Mass transfer operations and equipment:

Distillation: Principle, distillation of binary mixture, Raoult’s law,

Methods of distillation: Differential and steam distillation. (PO1: M:

Apply the knowledge of mathematics, science, engineering

fundamentals)

Construction and working of distillation column. Estimation of number

of theoretical plates by Mc Cabe and Thiele method. Numerical problems.

(PO2: H: Identify and analyze complex engineering problems

reaching substantiated conclusions using first principles of

mathematics) 8 Hrs

Unit V

Extraction and drying: Liquid extraction, liquid liquid equilibria, choice

of solvent, single stage extraction, multi stage cross current extraction.

Drying: Batch and continuous drying operations, drying rate curve,

classification of drying equipment – direct driers, indirect driers, freeze

drying, rotary driers, drum driers, spray driers. (PO1: H: Apply the

knowledge of mathematics, science, engineering fundamentals)

8 Hrs

TEXT BOOKS:

1 Pauline M. Doron

Bioprocess Engineering Principles, 2nd Edition, 2013, 978-0-12-220851-5

2 McCabe W.L. and Smith J.C.

Unit operations in Chemical Engineering. McGraw-Hill, New York, E7th Edition, 1993, ISBN 007-

124710-6



REFERENCE BOOKS

1 Coulson and Richardson.

Chemical Engineering. Vols. 1, 2 & 4, Butterworth-Heinemann Ltd, 3rd Edition 1999,

0750641428

2 Robert E Treybal Mass Transfer Operations Vol 2, Issue 4, McGraw Hill Education,3rd Edition, 1981, 1259029158


1. Apply the concepts of conduction to determine steady-state

unidirectional rate of heat transfer through different geometries.

2. Apply the concepts of convection in heat transfer equipment such as

double pipe heat exchanger and shell and tube heat exchanger.

3. Apply the concepts of diffusion to identify and formulate criteria for

selecting alternative separation technologies.

4. Apply the basic concepts of distillation and determine the number of

plates required for distillation.

5. Understand the concepts of various mass transfer operations like

extraction and drying.



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBT05 2,3 2,3 1,2


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Apply the concepts of conduction to determine steady-state unidirectional rate of heat transfer through different geometries.

2 1



Apply the concepts of convection in heat transfer equipment such as double pipe heat exchanger and shell and tube heat exchanger

2 3 2

Apply the concepts of diffusion to

identify and formulate criteria for

selecting alternative separation

technologies.

2 2 2

Apply the basic concepts of distillation and determine the number of plates required for distillation

2 2

Understand the concepts of various mass transfer operations like extraction and drying.

3 2




MOLECULAR BIOLOGY LABORATORY

Lab Hours/ Week : 0+0+3 (L+T+P) Credits: 1.5

Course Code : 4RBTL01 CIE Marks: 50

SEE Marks: 50


1. Study the important techniques involved in Molecular Biology.

2. Study the divisional stages of cell.

3. Study the isolation of genomic DNA from plant, animal and

microbial source.

4. Involve the use of recombinant DNA technology and other Common

gene analysis techniques.

5. Will develop basic knowledge and skills in cell and molecular

biology and become aware of the complexity and harmony of the

cell.


1. Study of divisional stages in mitosis (squash preparation and

staining–specimen: Onion root tip).

2. Study of divisional stages in meiosis (Permanent slides only).

3. Study of Drosophila mutants, Barr eye, vestigial wings, yellow

body, white eye.

4. Polytene chromosomes, Lampbrush chromosomes – permanent

slides.

(PO1: M, Knowledge transfer: Knowledge of basic science)

5. Isolation of plant protoplasts by enzymatic method and its fusion.

(PO2-L: To gather knowledge and applying the knowledge in

identifying the biological use of the technique)

6. Isolation of plasmid DNA from E.coli.

7. Isolation of genomic DNA from Plant.

8. Isolation of genomic DNA from animal source.

9. Isolation of genomic DNA from microbial source.


identifying the suitable protocol to perform experiment, PO6-L

Imbibing professional engineering practices, PO5-M: Use of modern

equipment)

10. Agarose gel electrophoresis and quantification of nucleic acids

(260/280 method spectrophotometric method).



11. Restriction mapping (Single or double digestion).

12. Study of SDS-PAGE.

13. PCR.



Imbibing professional engineering practices, PO5: Use of modern

equipment)

14. Study of conjugation in E.coli – Kit method.

15. Transformation of E.coli cells – Calcium chloride method (Kit may

be used). Selection of recombinants (Blue-White screening).



Imbibing professional engineering practices, PO5: Use of modern

equipment)

TEXT BOOKS:

1 H S Chawla Introduction to Plant Biotechnology, Oxford

and IBH Publication, New Delhi, 3rd

Edition, 2017, 945-0299108236

2 F.M. Ausubel, R. Brent,

R.E. Kingston, D.D.

Moore, J.G. Seidman,

J.A. Smith, and K.

Struhl

Current Protocols in Molecular Biology,

Green Publishing Associates, and Wiley-

Interscience John Wiley and Sons, New

York, 1st Edition, 1987, 989-0299108245

REFERENCE BOOK:

1 Primates Principles of Gene Manipulation and

Genomics, Wiley-Blackwell Publishers, 7th

Edition, 2006, 945-2599108245

Course Outcomes:

After the completion of this course, students will be able to:

1. Memorize and understand the divisional stages of cells, Drosophila

mutants, and special forms of chromosomes. (BL:L1) (PO1-M, PO5- L,

PSO1-L, PSO3-L)

2. Describe the process of isolation of plant protoplast with their use in

molecular biology experiments. (BL: L2) (PO2-M, PSO1-L, PSO3-L)



3. Discuss and execute DNA isolation from various sources and interpret

the advantages and disadvantages of each method. (BL: L2, L3) (PO2-

M, PO5-L, PO6-L, PSO1-L, PSO3-L)

4. Explain the use of electrophoresis, restriction mapping and DNA

amplification techniques in various molecular biology experiments.

(BL: L2, L3) (PO2- M, PO5- M, PO6-L, PSO1-L, PSO3-L)

5. Define, classify and execute bacterial conjugation, transformation and

identify the developed recombinants using cell specific media. (BL: L2,

L3) (PO2-M, PO5- H, PSO1-L, PSO3-L)



Course

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

4RBTL01 2 1 2 1 1 1


Course outcomes


PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

7

PO

8

PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Memorize and understand the divisional stages of cells, Drosophila mutants, and special forms of chromosomes.

2 1 1

Describe the process of isolation of plant protoplast with their use in molecular biology experiments.

1 1 1

Discuss and execute DNA isolation from various sources and interpret

the advantages and disadvantages of each method.

1 2 1 1 1

Explain the use of electrophoresis, restriction mapping and DNA amplification techniques in various molecular biology experiments.

1 2 1 1 1

Define, classify and execute bacterial conjugation, transformation and identify the developed recombinants using cell specific media.

1 2 1 1 1



TRANSPORT PROCESSES LABORATORY Lab Hours/ Week : 0+0+3 (L+T+P) Credits : 1.5

Course Code : 4RBTL02 CIE Marks : 50

SEE Marks : 50

Course objectives: This course will enable students to: 1. Learn basic Momentum transfer concept unit operations of

Chemical engineering.

2. Understand operations of flow measuring devices.

3. Hands on training in unit operations of Chemical engineering.

4. Exposure to mass transfer operations.

5. Study the basic concept of heat transfer in Heat exchangers.


1. Flow through circular conduits.

(PO1: M, Knowledge transfer: Knowledge of Chemical engineering)

(PO2: M Evaluates the analysis of accuracy and validity of

assumption made) (PO4: L Analyse the data including considering

the source of error)

2. Orifice meter.

3. Venturi meter.


(PO2: M Evaluates the analysis of accuracy and validity of

assumption made)

4. Simple distillation. 5. Steam distillation. 6. Packed column distillation.

7. Single and Multistage extraction. 8. Double pipe heat exchanger.

9. Shell and tube heat exchanger. 10. Thermal conductivities of insulating materials. 11. Centrifugal Pump 12. Diffusivity Measurement.

13. Batch Dryer. 14. Adsorption.




(PO2:M Evaluates the analysis of accuracy and validity of

assumption made)

TEXT BOOKS:

REFERENCE BOOK:

1 Coulson J. II and

Richardson J.F.

Chemical Engineering.Vol.1.Asian Books. New

Delhi. 6th Edittion, 1998, 945-0-11-230853-2

2 Badger W.I. and

Banchero J.T.

Introduction to Chemical Engineering, Tata

McGraws-Hill. New York, 6th Edition, 2001, 956-0-

11-260253-4


able to:

1. Calculate frictional losses and pressure drops in piping system and

fluidized bed. (BL: L4) (PO1-M, PO2-M, PO4-L, PSO1-L)

2. Determine Coefficient of discharges in flow measuring devices of

Venturimeter and Orifice meter. (BL: L4) (PO1-M, PO2M, PSO1-L)

3. Determine the Rayleigh equation, performance efficiency and

number of stages in distillation. (BL: L4) (PO1-M, PO2M, PO4-L,

PSO1-M)

4. Analyse the effect of single stage and multiple stages in separation

of components in extraction. (BL: L4) (PO1-M, PO2M, PSO1-M)

5. Deduce the rate of heat transfer and area required in heat

exchanger. (BL: L4) (PO1-M, PO2M, PSO1-L)

1 McCabe W L

et. al.

Unit Operations of Chemical Engineering, McGraw

Hill. New York, 7th Edition, 1993, 945-0-12-220856-4

2 Kumar K L. Engineering Fluid Mechanics, Eurasia Publishing

House (p) Ltd., New Delhi, Ed77th Edition, 1995,

969-0-12-220823-4





Course

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1

PO

2

PO

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PO

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PO

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PO

6

PO

7

PO

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PO

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PO

10

PO

11

PO

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PSO

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PSO

2

PSO

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4RBTL02 2 2 1 1


Course outcomes


outcomes (PSOs)

PO

1

PO

2

PO

3

PO

4

PO

5

PO

6

PO

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PO

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PO

9

PO

10

PO

11

PO

12

PSO

1

PSO

2

PSO

3

Calculate frictional losses and pressure drops in piping system and fluidized bed

2 2 1 1

Determine Coefficient of discharges in flow measuring devices of Venturimeter and Orifice meter

2 2 1

Determine the Rayleigh equation, performance efficiency and number of stages in distillation

2 2 1 2

Analyse the effect of single stage and multiple stages in separation of components in extraction

2 2 2

Deduce the rate of heat transfer and area required in heat exchanger

2 2 1




ENVIRONMENTAL SCIENCE



Course Code : HSS05 SEE Marks : 50


This course introduces the students to the problems of depletion of

natural resources due to deforestation, agricultural practices, and

adverse environmental effects, pesticides, soil erosion, mining. Different

types of energy- renewable, non-renewable and energy conservation,

impact of environmental pollution on water quality, air quality, soil

pollution and noise pollution, solid waste management- disposal,

treatment of different types of solid waste including MSW, e-waste,

biomedical waste, societal impact of environmental issues- ozone layer

depletion, GHG effects, water conservation and harvesting and

environmental protection & acts.

Unit I

Introduction:

• Components of Environment and their interactions • Ecology, Ecosystem and types

Natural Resources:

• Forest Resources-Deforestation, Causes of deforestation, Environmental effects of deforestation and solutions

• Water resources, World’s water reserves, Hydrological cycle

• Land resources, Land degradation. Soil erosion, Causes and prevention, Soil conservation and its types

• Mineral resources of India, Mining & its adverse effects

Numerical problems on rainfall & runoff

6 Hrs



Unit II

Energy and resources:

• Types of Energy-Renewable, Non renewable & sustainable energy & their advantages and disadvantages

• Renewable energy sources- Solar energy, Wind energy, Tidal energy, Ocean thermal energy. Geothermal energy, Hydroelectric power, Biomass energy, Hydrogen energy, Thermal power- environmental impacts

• Conservation of energy • Numerical problems on Solar energy, Wind power 5 Hrs

Unit III

Environmental pollution:

• Sources of pollution- Natural sources, & Anthropogenic • Pollutants- Classification & their effects on environment • Air pollution-Composition of clean air, Sources of air pollution & Air

pollutants, Effect of air pollution on humans, animals and plants &

climate • Water quality–Potable water, Wholesome water, Sources of water

pollution Polluted water & Contaminated water, • Common impurities in water (physical, chemical and bacteriological),

Effects of impurities on humans & industrial use • Soil Pollution-Sources, Effects & its control

• Noise pollution- Sources of noise, Effects on human health & its control

Numerical problems on pH, hardness of water, noise pollution 5 Hrs

Unit IV

Solid Waste Management:

• Refuse, Garbage, Rubbish, Ash, types of solid waste • Necessity of safe disposal, Impacts on human health and

environment • Classification of solid wastes- Quantity and composition of MSW,

Collection of solid waste- methods • Disposal of solid waste-Sanitary land-fill • E-waste- Problems and solutions • Biomedical waste-Impacts on human health, storage, treatment

methods and disposal Numerical problems on moisture content, density & area land fill

5 Hrs



Unit V

Sustainable development:

• Issues on energy utilization, water conservation, concept of 3 Rs, Rain water harvesting- methods

• Global environmental issues: Population growth, Urbanization, Global warming, Acid rains, Ozone layer depletion & controlling measures

• Environmental acts, Regulations, Role of state & central governments

• Introduction to GIS & Remote sensing, their applications in

environmental engineering practices

Numerical problem on carbon foot print & rainwater harvesting 5 Hrs

TEXT BOOKS:

1 Benny Joseph Environmental Studies, The McGraw-Hill

companies, 4th Edition, 2005, 978-0073532554

2 Snathosh Kumar Garg, Rajeshwari Garg and Dr Ranjani Garg

Ecological and Environmental Studies, Khanna Publishers, 3rd Edition, 2010, 987-0078532523

REFERENCE BOOK:

1 Erach

Bharucha

Environmental studies for Undergraduate Courses, 1st

Edition, 2013, University Press, 923-0024532545


1. Describe the importance of forestation, effects of deforestation, land

degradation, adverse effects of mining on environment, using the

principles of natural sciences compute the runoff from rainfall &

estimates the conservation of water for beneficial use of humans.

2. Describe the Renewable sources of energy and formulate, review

literature, calculate power potential of solar & wind energy by using

the principles of natural sciences.

3. Describe the effects of pollution on air, water, soil & noise on

humans and environment, identify & analyze the pollution

problems related to air, water, soil & noise and quantify pollution

levels & draw valid inferences using engineering sciences.



4. Describe Impact of solid waste on human health and environment,

its safe disposal. Use population data & compute percapita solid

waste generation, land area requirement for sanitary landfill.

5. Describe the sustainable development, its importance, current

global environmental issues, Present state & central governments

protection acts, compute carbon foot print using

data(vehicles/industries) & asses its impact on environment.

ENGINEERING MATHEMATICS – III

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