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Label-free interaction analysis

Biacore™ systems for more information and faster decisions

More answers: Deeper insights into biological realitiesBiological processes are “real-time” events, driven and regulated by dynamic interactions between key molecules.

End-point techniques such as ELISA offer a snapshot view of interactions providing only basic information such as overall binding strength (affinity). The affinity depends on the ratio of on- and off-rates so that equal affinity interactions can have very different kinetic properties, resulting in different biological responses.

Biacore systems can provide key data in real-time to discriminate these crucial differences, even for interactions where challenging targets are involved.

Biacore systems are designed to help you to generate decisive, information-rich data, that will help answer key questions concerning the nature of the binding:

1. How strong? Affinity is a measure of the strength of binding/attraction between molecules. Affinity is a time independent measurement and is determined by measuring the binding response at steady state (constant signal).

2. How fast? Binding kinetics determine how fast/slow a complex forms or dissociates within a given time and allow calculation of association and dissociation rate constants.

3. How much? Qualitative and quantitative determination of active analyte binding to a target protein vs total protein concentration, which is obtained via classic absorbance measurement at 280 nm wavelength.

4. How specific? Is the molecule specific for its target? Does the antibody recognize multiple derivatives? The flexibility in the Biacore assay design allows rapid assessment of cross-reactivity and specificity.

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More insights: Real-time monitoring of binding events delivers deeper understandingBiacore systems provide valuable information from high-quality molecular interaction data in a range of fields: from biological research, biotherapeutics and small-molecule drug discovery and development, through immunogenicity studies and vaccine development to quality control. The sensitivity and performance of Biacore systems enables detailed characterization of molecular interactions even for challenging targets such as membrane proteins. Biacore systems allow you to:

• Understand the relationship between molecular interaction and function.

• Screen and characterize for hits and optimize leads based on selectivity, affinity, and kinetics.

• Study interactions of ions, small molecules, and multidomain proteins or viruses with targets.

• Screen and characterize antibodies and proteins based on yes/no binding, affinity, and kinetics from the fastest on-rates to the slowest off-rates.

• Quantitate protein by measuring the concentration of active protein with retained biological function.

• Validate results from other techniques/approaches such as ELISA, HTS, or other biophysical methods.

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Biacore systems

Fields

• Basic research

• Biotherapeutics discovery and development

• Small-molecule discovery

• Biotherapeutics manufacturing and quality control

• Vaccine design and development

Molecules

• Antibodies and antibody variants

• Proteins including multidomain proteins

• Peptides

• Compounds and fragments

• Nucleic acids

• Ions

• Viruses

• Aptamers

• G protein-coupled receptors (GPCRs)

• Membranes

Methods

• Affinity

• Kinetics

• Screening

• Specificity

• Concentration

• Epitope binning

• Immunogenicity

• Thermodynamics

• Competition assays

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Association: Interactants in solution bind to molecules on sensor chip surface.

Association Dissociation

The binding (association) and dissociation of molecules to the sensor chip surface are monitored in real-time and displayed in a sensorgram.

Dissociation: Binders allowed to dissociate from molecule on sensor chip surface.

More information: Biacore systems provide high-quality data in key applications

Characterization of molecular interactions

Boost your protein research and enable the design of new and innovative studies.

• Real-time monitoring of binding events gives a deeper understanding of the dynamics of molecular interactions.

• Kinetics, affinity, transition state thermodynamics, and yes/no binding data help you prove your hypotheses, understand the mechanism of action, and make dependable conclusions.

Structural/functional studies Screening Characterization

Research Biotherapeutics and small-molecule discovery

Application and technical support – Training – Instrument service – Validation services

Screen and optimize your compounds based on direct label-free binding data.

• Screen and select hits based on selectivity, affinity, and kinetics.

• Optimize lead compounds through increased understanding of molecular mode of action.

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Lead optimization Critical quality attributes (CQA)

Potency

Biotherapeutics and small-molecule discovery

Release testing

Biotherapeutics development and manufacturing

Application and technical support – Training – Instrument service – Validation services

Understanding the nature of interactions between molecules is fundamentally important for increased understanding of biological processes. Biacore surface plasmon resonance (SPR)-based sensors are widely used to study molecular interactions in real-time and are applied as powerful tools in research and drug discovery throughout to quality control and drug-release testing.

Design your vaccines and biotherapeutics with desired binding properties.

• Accurate ranking and selection of antibodies and proteins based on yes/no binding, affinity, and kinetics—from very fast on-rates to very slow off-rates.

Reliably define CQA and monitor drug release parameters.

• Fingerprint analysis of drug interaction and stability, even for new drug formats such as bispecific antibodies.

• Novel opportunity for comparability and biosimilar studies.

• Seamless integration into GxP-regulated workflows.

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Get more from your systemBiacore service and support

GE is committed to supporting customers with innovative tools, analytics, and everyday lab reagents to uncover new insights in research or to discover, develop and manufacture drugs.

More than 30 000 scientific publications and still counting...Thousands of Biacore systems are installed globally and more than 30 000 scientific articles have been published in peer-reviewed journals where Biacore systems have been featured for a wide range of applications.

Experienced application specialists and service expertsOur instrument service and application support is performed by specially trained service experts available close to you. They can help you to get the most out of your Biacore system for all applications and improve efficiency by minimizing your equipment system downtime. This allows you to focus on your work delivering reliable binding analysis results. To learn more about assay setup and data interpretation, GE offers a wide range of self-training tools, classroom trainings, handbooks, and lab protocols.

Join our family—Biacore communityAs an owner of a Biacore system, you are connected to a world of knowledge and experience in interaction analysis.

• Easy access to information about your Biacore system.

• Ability to explore a rich content library including videos and handbooks.

• Exclusive offers on Biacore products.

• Access to presentations and materials from our regional user days and DiPIA conferences.

Join Biacore community, share your experiences, and learn more at regional user days and DiPIA conferences.

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Consumables and software that deliver moreGE Healthcare Life Sciences has developed a range of tools designed specifically to make Biacore assays as easy and reliable as possible, suitable for users of all levels of experience. The complete toolbox is backed by stringent development, production methods and quality control. Selected Biacore systems facilitates compliance with worldwide regulatory expec-tations by offering Validation Services and 21 CFR Part 11 functionality. Get started quickly at any experience level Use predefined Biacore run methods with application-relevant settings to rapidly set up your assay, or utilize the full flexibility of the control software to tailor methods to your specific needs.

Reduce time to conclusive resultsThe efficient evaluation software is designed to provide data overview, speed up data eval-uation, and to shorten time to results. Quality control tools help you to assess high-quality kinetic and affinity data for improved interpretation of results. A sensor surface for every need Biacore sensor chips offer support for analysis of a wide range of interactions, backed up with a selection guide to help you select and use the right tools for the application. Save time and effort using kits and ready-made consumablesOur capture kits significantly reduce assay development time and gives consistent capture of antibodies as well as the most common tags. Ready-made buffers and solutions are developed for your convenience ease of use, and to ensure reliable results.

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Biacore 8K+

High sensitivity with maximized capacityHigh-sensitivity SPR system with the highest capacity available in the Biacore system portfolio. For use in high-throughput screening, characterization, process optimization, and quality control.

Biacore 8K

High sensitivity and efficiencyHigh sensitivity and high-throughput SPR system, delivering high-quality binding data to meet the toughest challenges in screening, characterization, and quality control.

Biacore S200

Outstanding sensitivity for cutting-edge applicationsOur most sensitive SPR-based sensor enables confident analysis of the smallest fragments, even against challenging targets such as GPCRs in LMW/fragment drug discovery.

More choices for label-free interaction analysisFrom research through drug discovery and development to QC. Choose the right system for your needs.

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Biacore T200

Reliable, versatile, and preciseVersatile system for high-quality characterization of molecular interactions—from ions to viruses.

Biacore X100

Reliable, built-in knowledge baseReliable insights into biological processes in multiuser environments/small-scale interaction analysis.

Biacore C

Quality, compliant, and secureThe world’s first SPR instrument dedicated to rapid determination of concentration in GxP environments.

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