Praktikum Mikroskop

7/17/2019 Praktikum Mikroskop

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Praktikum Mikroskop

Peni KS Mutalib


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Goals

Being skilled to use microscope

To know some important principle so that

student could operate microscope: good inshort time.

Determine alternati procedure which bewanted

Making adaptor sel and taking photo!optional"


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#ssignment in Practice Microscope$anuar% &''(

). Mention the parts o a microscope*&. +hat is the unction o those parts,-. ow to optimali/e the unctions,0. +hat should be done to ma1imi/e %our 2P,

3. +hat is the 4# o %our microscope,5. #nd rom %our right and let hand side microscope,6. 2P o no. 3 and 5: what is better, +hat is %our argument,(. 7igure the tissue with low8 medium and high power

!magniication o ob9ectie )'18 0'18 and )''1"* +rite theoerall magniication )''18 0''18 )'''1.;. 2eport %our e1periences in using microscope: The steps*)'.+hat is the dierence with the steps mention in theor%,


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Micoscope 7igure


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Seeral theor%

2esoling Power

4#: 4umerical #perture

?mmersion oil ow to use =ondensor


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>ens protection and putting slidewhile

Bringing microscope

=leaning lens

7ocusing small ob9ectie power 01 andmedium )'1

Stud%ing slides with high power 0'@031

or when using immersion oil 2apikan mik setelah selesai *


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?denti% part o the microscope Do it seeral times8 think about the unction


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Dierent location o coarse and ine ad9ustment


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7ine a9ustment

t t th b t 2P


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ow to get the best 2P rom amicroscope incl. how and when to

do:

=leaning the lenses

?lluminate the specimen 7ocusing the condenser

#9ust the diagragma

Asing immersion

Setting light intensit%


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Magniication 4umeric #perture


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4# C n sin


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2esoling Power

2esolution o lens camera is reealed b% how man% lines

per inch !lpi"


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>ens resolution ?s the abilit% o a lens to ree shadow rom

& point o thing that is er% close. 2esolusion limit C ).&&D !2esolusion depend

on "

Da%a urai !C2esoling PowerE2PEs"C C).&&D !DC&sin" where C9arak ob9ek kelensa ob9ekti

How smaller how smaller RP

How larger Dhow smallerRP

How bigger

how smaller RP


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>ight source


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>ong wae length hae greaterenerg%


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ec romagne c waespectrum
http://ethel.as.arizona.edu/~collins/astro/subjects/electromag9.htmlhttp://ethel.as.arizona.edu/~collins/astro/subjects/electromag9.html


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Step in ocusing microscope


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Step


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Step

The best 2P isual microscope &''


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The best 2P isual microscope @ &''nm

Magnification is not the same with RP

RP microscope mainly depend on obj lens (oculer-max 1x!

only magnify imagewhich is found from obj lens-max 1x"#

$culer lens didn%t ma&e better the resolusion# Higher

magnification with low resolutionimage blur which is '

function

)* n sin

RP*

+#

)

, obj

$n the objectie was wrote Plan ./0#/

(Magnification0)"

120#134ube length in mm

4hic&ness(in mm" dec& glass for which obj lensis corected dr field

RP *!21

)*

(!21"(/!/ x 1-3m

1!2

. x 1 53m .

nmn*1!6 (n immersion oil"

sin !7


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>arge !7ast" 4# s. Small !slow" 4#


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Thus8 4#:


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=ondensor

Make better 2P with making light lamp)''H o the image@orming kind

ow: with ocusing light in the shape oinerse cone8 with ape1 at the specimenand base at the ob9ectie


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Modern 2esearchMicroscope


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Tem o8 && $uni &''-: hal. )5


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4# ).0'

I


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==D camera ASI-3''

MPi1el ,

Praktikum Mikroskop

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