PERBEDAAN TITER SALMONELLA TYPHI-O DAN SALMONELLA

TYPHI H MENGGUNAKAN PLASMA DAN SERUM

Herlina1, Sri Darmawati2, Budi Santosa2

1. Program Studi D IV Analis Kesehatan Fakultas Ilmu Keperawatan dan Kesehatan

Universitas Muhammadiyah Semarang.

2. Laboratorium Patologi Klinik Fakultas Ilmu Keperawatan dan Kesehatan Universitas

Muhammadiyah Semarang.

ABSTRAK

Uji Widal merupakan tes aglutinasi untuk diagnosis serologi penyakit demam tifoid atau

demam enterik. Uji Widal mengukur level aglutinasi antibodi terhadap antigen O

(somatik) dan antigen H (flagellar). Uji biasanya dilakukan pada serum akut, yaitu serum

yang pertama kali diambil saat pertama kali kontak dengan pasien, minimal harus

didapatkan 1 ml darah untuk mendapatkan jumlah serum yang cukup. Bahan pemeriksaan

uji Widal adalah serum, namun karena keterbatasan volume darah, sekaligus menghemat

waktu pemeriksaan, banyak laboratorium menggunakan sampel plasma sebagai alternatif.

Tujuan penelitian untuk mengetahui perbedaan titer Salmonella typhi-O dan typhi-H

menggunakan plasma dan serum. Jenis penelitian analitik pendekatan cross sectional.

Penelitian terhadap 16 sampel penelitian diperiksa menggunakan bahan serum dan

plasma. Hasil penelitian sampel serum diperoleh Salmonella typhi-O negatif,titer 1/80,

titer 1/160, titer 1/320 berturut-turut sebanyak 2,6,6,2 sampel. Salmonella typhi-H titer

1/80, titer 1/160, titer 1/320 berturut-turut sebanyak 1, 1,11,3 sampel. Sampel plasma

diperoleh. Salmonella typhi-H negatif, titer 1/80, titer 1/160, titer 1/320 berturut-turut

sebanyak 1,7,6,2 sampel. Salmonella typhi-H titer 1/80, titer 1/160, titer 1/320 berturut-

turut sebanyak 1, 13, 2 sampel. Hasil uji statistik dengan Wilcoxon dan Chi Square

diperoleh p > 0,05 yang berarti tidak terdapat perbedaan bermakna Salmonella typhi-O

dan typhi-H sampel serum dan plasma.

Kata kunci : Salmonella typhi O, Salmonella typhi H, serum, plasma

repository.unimus.ac.id

THE DIFFERENCE OF SALMONELLA TYPHI-O AND SALMONELLA

TYPHI H TITER USING PLASMA AND SERUM

Herlina1, Sri Darmawati2, Budi Santosa2

1. Study Program of D IV Health Analyst Faculty of Nursing and Health University of

Muhammadiyah Semarang.

2. Clinical Pathology Laboratory Faculty of Nursing and Health Sciences University of

Muhammadiyah Semarang.

ABSTRACT

Widal test is agglutination test for serological diagnosis of typhoid fever or enteric fever.

Widal test measures the agglutination level of antibody towards O antigen (somatic) and

H antigen (flagellar). The test is usually performed on acute serum, that is first serum

which taken at the first time contact with patients, at least must be obtained 1 ml of blood

to get enough serum. Widal testing material is serum, but due to the limited blood

volume, while saving the examination time, many laboratories using plasma samples as

an alternative. The research goal is to know the difference of Salmonella typhi-O and

typhi-H titer using plasma and serum. The research type is analytical with cross sectional

approach. The research towards 16 research samples were examined using serum and

plasma materials. The research result was serum samples obtained negative Salmonella

typhi-O, titer 1/80, titer 1/160, titer 1/320 consecutive as much as 2,6,6,2 samples.

Salmonella typhi-H titer 1/80, titer 1/160, titer 1/320 consecutive as much as 1, 1,11,3

samples. Plasma samples were obtained. Negative Salmonella typhi-H, titer 1/80, titer

1/160, titer 1/320 consecutive as much as 1,7,6,2 samples. Salmonella typhi-H titer 1/80,

titer 1/160, titer 1/320 consecutive as much as 1, 13, 2 samples. The statistic test result

with Wilcoxon and was Chi Square obtained p > 0,05 which means there was no

significant difference of Salmonella typhi-O and typhi-H serum and plasma samples.

Keywords: Salmonella typhi O, Salmonella typhi H, serum, plasma

repository.unimus.ac.id