LAMPIRAN Lampiran 1. Kurva Standar Protein Terlarut dengan ...

25

LAMPIRAN

Lampiran 1. Kurva Standar Protein Terlarut dengan Metode Biuret

BSA (mg/mL) Absorbansi

0 0,000

1 0,055

2 0,085

3 0,111

4 0,146

5 0,173

6 0,193

7 0,233

8 0,257

9 0,281

10 0,314

26

Lampiran 2. Data dan Contoh Penghitungan Protein Terlarut Metode

Biuret dengan menggunakan Kurva Standar

� Data Pengaruh Variasi Penambahan Usar TempeTerhadap Kadar

Protein Terlarut

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 0,384 0,393 0,314 0,295

2 0,385 0,397 0,312 0,297

3 0,385 0,394 0,314 0,291

4 0,386 0,393 0,309 0,294

5 0,382 0,392 0,317 0,292

6 0,381 0,39 0,321 0,29

� Perhitungan Pengaruh Variasi Penambahan Usar Tempe dan Tepung

Belut Terhadap Kadar Protein Terlarut

A550 Perlakuan Ragi : Belut =1:0, ulangan 1 = 0,384

Persamaan kurva standar : y = 0,029x + 0,0018

y = Absorbansi sampel pada 550 nm

x = Konsentrasi Protein Terlarut

y = 0,029x + 0,0018

0,384 = 0,029x + 0,0018

x = 12,621 mg/mL

Kadar Protein Terlarut = 12,621 mg/mL

27

Lampiran 2. (Lanjutan)

� Analisis Sidik Ragam Pengaruh Penambahan Usar Tempe terhadap

Protein Terlarut

Ulangan

Perlakuan

Tu Xu du R:B =

1:0

R:B =

1:1

R:B =

2:1

R:B =

3:1

1 12,621 12,931 10,207 9,552 45,311 11,3278 0,0116

2 12,655 13,069 10,138 9,621 45,483 11,3708 0,0546

3 12,655 12,966 10,207 9,414 45,242 11,3105 -0,0056

4 12,690 12,931 10,034 9,517 45,172 11,2930 -0,0231

5 12,552 12,897 10,310 9,448 45,207 11,3018 -0,0144

6 12,517 12,828 10,448 9,379 45,172 11,2930 -0,0231

Tp 75,690 77,622 61,344 56,931 271,587

Xp 12,6150 12,9370 10,2240 9,4885 (T..) 11,31613

dp 1,2989 1,6209 -1,0921 -1,8276 (X..)

SD 0,0670 0,0798 0,1426 0,0912

SE 0,0703 0,0837 0,1497 0,0957

Keterangan : Tp = Total Perlakuan ; Tu = Total Ulangan ; T.. = Total Umum ;

X p = Purata Perlakuan ; X u = Purata Ulangan ; X .. = Purata Umum

dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (12,621)(1,2989) + (12,931)(1,6209) + (10,207)(-1,0921) +

(9,552)(-1,8276)

= 8,7478

W2 = (12,655)(1,2989) + (13,069)(1,6209) + (10,138)(-1,0921) +

(9,621)(-1,8276)

= 8,9649

W3 = (12,655)(1,2989) + (12,966)(1,6209) + (10,207)(-1,0921) +

(9,414)(-1,8276)

= 9,1009

28


W4 = (12,690)(1,2989) + (12,931)(1,6209) + (10,034)(-1,0921) +

(9,517)(-1,8276)

= 9,0904

W5 = (12,552)(1,2989) + (12,897)(1,6209) + (10,310)(-1,0921) +

(9,448)(-1,8276)

= 8,6807

W6 = (12,517)(1,2989) + (12,828)(1,6209) + (10,448)(-1,0921) +

(9,379)(-1,8276)

= 8,4988

N = � W. du

= (8,7478)(0,0116) + (8,9649)(0,0546) + (9,1009)(-0,0056) +

(9,0904)(-0,0231) + (8,6807)(-0,0144) + (8,4988)(-0,0231)

= 0,0087

D = ��22 . dudp

= {( 1,2989)2 + ( 1,6209)2 + (-1,0921)2 + (-1,8276)2} x {(0,0116)2 +

(0,0546)2 + (-0,0056)2 + (-0,0231)2 + (-0,0144)2 + (-0,0231)2}

= 0,0392

FK = ( ) ( ) ==

24271,587.. 22

observasijumlahT

3073,3124

JK Total = � − FKX 2

= {(12,621)2 + (12,655)2 + ... + (9,379)2} – 3.073,3124

= 53,2811

29


JK Perlakuan = ulanganjumlah

Tp�2

- FK

= 3124,073.36

)931,56(....)690,75( 22

−++

= 53,0836

JK Kelompok = perlakuanjumlah

Tu�2

- FK

= 3124,073.34

)172,45(....)311,45( 22

−++

= 0,0177

JK Galad Acak = JK Total – JK Perlakuan – JK Kelompok

= 53,2811 – 53,0836 – 0,0177

= 0,1798

JK Non Aditivitas = D

N 2

= 0,0392

)0087,0( 2

= 0,0019

JK Sisa = JK Galad Acak – JK Non Aditivitas

= 0,1798– 0,0019

= 0,1779

30


DASIRA UJI ADITIVITAS

Sumber Db JK KT FHitung Ftabel

Ragam 5% 1%

Ulangan 5 0,0177

Perlakuan 3 53,0836

Galad acak 15 0,1798

Non aditivitas 1 0,0019 0,0019 0,1513 4,6 8,36

Sisa 14 0,1779 0,0127

F tabel 1 % > F tabel 5 % > F hitung � data aditif

DASIRA


Ragam 5% 1%

Ulangan 5 0,0177 0,0035 0,2955 2,56 4,36

Perlakuan 3 53,0836 17,6945 1476,4128** 3,29 5,42

Galad acak 15 0,1798 0,0120

Total 23 53,2811

Fhitung ulangan < Ftabel 5% < Ftabel 1% � Antar ulangan tidak berbeda nyata

Fhitung perlakuan > Ftabel 5% < Ftabel 1% � Antar perlakuan berbeda sangat nyata

Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0120

Sx

= = 0,0447

W = 4,08 × 0,0447 = 0,1824

31


KV = −X..

acak galad KT x 100%

= 316,11

0,0120x 100%

= 0,97 %

Purata Kadar Protein Tempe (mg/mL ± SE) pada Perlakuan Variasi

Penambahan Usar Tempe

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

Purata ± SE 12,6138 ± 0,0785 12,9370 ± 0,0837 10,2240 ± 0,1497 9,4885 ± 0,0957

W=0,1824 (c) (d) (b) (a)

Keterangan:Angka-angka yang diikuti oleh huruf yang sama menunjukkan antar perlakuan tidak berbeda

secara bermakna, sedangkan angka yang diikuti oleh huruf yang berbeda menunjukkan antar

perlakuan berbeda secara bermakna,

W = BNJ 5%

� Data Pengaruh Variasi Penambahan Tepung Belut Terhadap Kadar

Protein Terlarut

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 12,621 12,931 13,207 14,034

2 12,655 13,069 13,103 14,172

3 12,655 12,966 13,207 14,724

4 12,69 12,931 13,276 14,172

5 12,552 12,897 13,207 14,138

6 12,517 12,828 13,172 14,793

32


� Analisis Sidik Ragam Pengaruh Variasi Penambahan Tepung Belut

Terhadap Protein Terlarut

Ulangan

Perlakuan

Tu Xu du R:B =

1:0

R:B =

1:1

R:B =

1:2

R:B =

1:3

1 12,621 12,931 13,207 14,034 52,793 13,1983 -0,0733

2 12,655 13,069 13,103 14,172 52,999 13,2498 -0,0218

3 12,655 12,966 13,207 14,724 53,552 13,3880 0,1165

4 12,69 12,931 13,276 14,172 53,069 13,2673 -0,0043

5 12,552 12,897 13,207 14,138 52,794 13,1985 -0,0730

6 12,517 12,828 13,172 14,793 53,31 13,3275 0,0560

Tp 75,69 77,622 79,172 86,033 318,517

Xp 12,6150 12,9370 13,1953 14,3388 (T..) 13,27154

dp -0,6565 -0,3345 -0,0762 1,0673 (X..)

SD 0,0670 0,0798 0,0565 0,3297

SE 0,0703 0,0837 0,0593 0,3461



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (12,621)(-0,6565) + (12,931)(-0,3345) + (13,207)(-0,0762) +

(14,034)(1,0673)

= 1,3597

W2 = (12,655)(-0,6565) + (13,069)(-0,3345) + (13,103)(-0,0762) +

(14,172)(1,0673)

= 1,4464

W3 = (12,655)(-0,6565) + (12,966)(-0,3345) + (13,207)(-0,0762) +

(14,724)(1,0673)

= 2,0621

33


W4 = (12,690)(-0,6565) + (12,931)(-0,3345) + (13,276)(-0,0762) +

(14,172)(1,0673)

= 1,4564

W5 = (12,552)(-0,6565) + (12,897)(-0,3345) + (13,207)(-0,0762) +

(14,138)(1,0673)

= 1,5274

W6 = (12,517)(-0,6565) + (12,828)(-0,3345) + (13,172)(-0,0762) +

(14,793)(1,0673)

= 2,2752

N = � W. du

= (1,3597)(-0,0733) + (1,4464)(-0,0218) + (2,0621)(0,1165) +

(1,4564)(-0,0043) + (1,5274)(-0,0730) + (2,2752)(0,0560)

= 0,1185

D = ��22 . dudp

= {(-0,6565)2 + (-0,3345)2 + (-0,0762)2 + (1,0673)2} x {(-0,0733)2 + (-

0,0218)2 + (0,1165)2 + (-0,0043)2 + (-0,0730)2 + (0,0560)2}

= 0,0471

FK = ( ) ( ) ==

24318,517.. 22

observasijumlahT

4227,2116


= {(12,621)2 + (12,655)2 + ... + (14,793)2} – 4227,2116

= 10,7411


Tp�2

- FK

= 2116,42276

)033,86(....)69,75( 22

−++

= 10,1273

34



Tu�2

- FK

= 2116,42274

)31,53(....)793,52( 22

−++

= 0,1116


= 10,7411– 10,1273– 0,1116

= 0,5022


N 2

= 0,0471

)1185,0( 2

= 0,2981


= 0,5022– 0,2981

= 0,2041



Ragam 5% 1%

Ulangan 5 0,1116

Perlakuan 3 10,1273


Non aditivitas 1 0,2981 0,2981 20,4533 4,6 8,36

Sisa 14 0,2041 0,0146

F tabel 1 % < F tabel 5 % < F hitung � data non aditif

Supaya aditif dicari dengan menggunakan data hilang

35


Ulangan Perlakuan Tu Xu

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 12,621 12,931 13,207 14,034 52,793 13,1983

2 12,655 13,069 13,103 14,172 52,999 13,2498

3 12,655 12,966 13,207 14,147 52,975 13,2438

4 12,69 12,931 13,276 14,172 53,069 13,2673

5 12,552 12,897 13,207 14,138 52,794 13,1985

6 12,517 12,828 13,172 14,043 52,56 13,1400

Tp 75,69 77,622 79,172 84,706 317,19 13,21625

(T..) (X..)

FK = ( ) ( ) ==

24317,19.. 22

observasijumlahT

4192,0623


= {(12,621)2 + (12,655)2 + ... + (14,043)2} – 4192,0623

= 7,6048


Tp�2

- FK

= 4192,06236

)706,84(....)69,75( 22

−++

= 7,5148


Tu�2

- FK

= 4192,06234

)56,52(....)793,52( 22

−++

= 0,0437

36



= 7,6048– 7,5148– 0,0437

= 0,0463

DASIRA


Ragam 5% 1%

Ulangan 5 0,0437 0,0087 2,8359* 2,56 4,36

Perlakuan 3 7,5148 2,5049 812,2355** 3,29 5,42

Galad acak 15 0,0463 0,0031

Total 23 7,6048

Ftabel 5% < Fhitung ulangan < Ftabel 1% � Antar ulangan berbeda nyata


Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0031

Sx

= = 0,0227

W = 4,08 × 0,0227 = 0,0926

KV = −X..


= 216,13

0,0031x 100%

= 0,42 %

37


Purata Kadar Protein Tempe (mg/mL ± SE) pada Perlakuan Variasi

Penambahan Tepung Belut

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

Purata ± SE 12,6150 ± 0,0703 12,9370 ± 0,0837 13,1953 ± 0,0593 14,1177 ± 0,0660

W=0,0926 (a) (b) (c) (d)




W = BNJ 5%

38

Lampiran 3. Data dan Contoh Penghitungan Kadar Lemak Kasar

� Data Pengaruh Variasi Penambahan Usar Tempe Terhadap Kadar

Lemak Kasar

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 5,86 11,13 9,5 7,96

2 6,27 11,2 9,92 8,46

3 5,33 11,2 9,73 7,4

4 5,56 11,2 10 7,8

5 5,67 10,8 9,78 7,9

6 5,73 10,8 9,75 7,95


Belut Terhadap Kadar Lemak Kasar

Kadar Lemak Kasar (%) = %1001

02 ×−

WWW

Keterangan:

W0= berat Kolf kosong

W1= berat sampel awal

W2= berat kolf kosong dan minyak

Contoh :

Kadar Lemak Kasar (%) = %10050

79,21472,217 ×−

= 5,86 %

39

Lampiran 3. (lanjutan)


Lemak Kasar

Ulangan

Perlakuan

Tu Xu du U:B =

1:0

U:B =

1:1

U:B =

2:1

U:B =

3:1

1 5,86 11,13 9,5 7,96 34,45 8,6125 -0,0083

2 6,27 11,2 9,92 8,46 35,85 8,9625 0,3417

3 5,33 11,2 9,73 7,4 33,66 8,4150 -0,2058

4 5,56 11,2 10 7,8 34,56 8,6400 0,0192

5 5,67 10,8 9,78 7,9 34,15 8,5375 -0,0833

6 5,73 10,8 9,75 7,95 34,23 8,5575 -0,0633

Tp 34,42 66,33 58,68 47,47 206,90

Xp 5,7367 11,0550 9,7800 7,9117 (T..) 8,6208

dp -2,8842 2,4342 1,1592 -0,7092 (X..)

SD 0,3165 0,1994 0,1731 0,3403

SE 0,3322 0,2093 0,1817 0,3571



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (5,86)(-2,8842) + (11,13)(2,4342) + (9,5)(1,1592) + (7,96)(-0,7092)

= 15,5582

W2 = (6,27)(-2,8842) + (11,2)(2,4342) + (9,92)(1,1592) + (8,46)(-0,7092)

= 14,6783

W3 = (5,33)(-2,8842) + (11,2)(2,4342) + (9,73)(1,1592) + (7,4)(-0,7092)

= 17,9209

W4 = (5,56)(-2,8842) + (11,2)(2,4342) + (10)(1,1592) + (7,8)(-0,7092)

= 17, 2869

W5 = (5,67)(-2,8842) + (10,8)(2,4342) + (9,78)(1,1592) + (7,7,9)(-0,7092)

= 15,6700

40


W6 = (5,73)(-2,8842) + (10,8)(2,4342) + (9,75)(1,1592) + (7,95)(-0,7092)

= 15,4267

N = � W. du

= (15,5582)( -0,0083) + (14,6783)( 0,3417) + (17,9209)( -0,2058) +

(17, 2869)( 0,0192) + (15,6700)( -0,0833) + (15,4267)( -0,0633)

= -0,7548

D = ��22 . dudp

= {(-2,8842)2 + (2,4342)2 + (1,1592)2 + (-0,7092)2} x {(-0,0083)2 +

(0,3417)2 + (-0,2058)2 + (0,0192)2 + (-0,0833)2 + (-0,0633)2}

= 2,7433

FK = ( ) ( ) ==

24206,90.. 22

observasijumlahT

1783,6504


= {(5,86)2 + (11,13)2 + ... + (7,95)2} – 1783,6504

= 97,9692


Tp�2

- FK

= 6504,17836

)47,47(....)42,34( 22

−++

= 96,5410

41



Tu�2

- FK

= 6504,17834

)23,34(....)45,34( 22

−++

= 0,6820


= 97,9692– 96,5410– 0,6820

= 0,7462


N 2

= 2,7433

)-0,7548( 2

= 0,2077


= 0,7462– 0,2077

= 0,5385



Ragam 5% 1%

Ulangan 5 0,6820

Perlakuan 3 96,5410


Non

aditivitas 1 0,2077 0,2077 5,3993 4,6 8,36

Sisa 14 0,5385 0,0385

F tabel 1 % > F hitung > F tabel 5 % � data non aditif


42



U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 5,86 11,13 9,5 7,96 34,45 8,6125

2 5,80 11,2 9,92 7,97 34,89 8,7225

3 5,33 11,2 9,73 7,4 33,66 8,4150

4 5,56 11,2 10 7,8 34,56 8,6400

5 5,67 10,8 9,78 7,9 34,15 8,5375

6 5,73 10,8 9,75 7,95 34,23 8,5575

Tp 33,95 66,33 58,68 46,98 205,94 8,5808

(T..) (X..)

FK = ( ) ( ) ==

24205,94.. 22

observasijumlahT

1767,1368


= {(5,86)2 + (11,13)2 + ...+ (7,95)2} – 1767,1368

= 100,7592


Tp�2

- FK

= 1767,13686

)98,46(....)95,33( 22

−++

= 99,9856


Tu�2

- FK

= 1767,13684

)23,34(....)45,34( 22

−++

= 0,2180

43



= 100,7592– 99,9856– 0,2180

= 0,5556

DASIRA


Ragam 5% 1%

Ulangan 5 0,2180 0,0436 1,1769 2,56 4,36

Perlakuan 3 99,9856 33,3285 899,7170** 3,29 5,42

Galad acak 15 0,5556 0,0370

Total 23 100,7592

Ftabel 5% > Fhitung ulangan > Ftabel 1% � Antar ulangan tidak berbeda nyata


Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0370

Sx

= = 0,0786

W = 4,08 × 0,0786 = 0,3207

KV = −X..


= 5808,8

0,0370x 100%

= 2,24 %

44


Purata Kadar Lemak Kasar Tempe (% ± SE) pada Perlakuan Variasi


U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

Purata ± SE 5,6583 ± 0,2011 11,0550 ± 0,2093 9,7800 ± 0,1817 7,8300 ± 0,2307

W=0,3207 (a) (d) (c) (b)




W = BNJ 5%


Lemak Kasar

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 5,86 11,13 11,27 13,13

2 6,27 11,2 11,27 13,67

3 5,33 11,2 11,4 14,13

4 5,56 11,2 11,53 13,33

5 5,67 10,8 11,28 13,65

6 5,73 10,8 11,35 13,2

45


� Analisis Sidik Ragam Pengaruh Penambahan Tepung Belut terhadap

Lemak Kasar

Ulangan

Perlakuan Tu Xu du

U:B =

1:0

U:B =

1:1

U:B =

1:2

U:B =

1:3

1 5,86 11,13 11,27 13,13 41,39 10,3475 -0,0675

2 6,27 11,2 11,27 13,67 42,41 10,6025 0,1875

3 5,33 11,2 11,4 14,13 42,06 10,5150 0,1000

4 5,56 11,2 11,53 13,33 41,62 10,4050 -0,0100

5 5,67 10,8 11,28 13,65 41,4 10,3500 -0,0650

6 5,73 10,8 11,35 13,2 41,08 10,2700 -0,1450

Tp 34,42 66,33 68,1 81,11 249,96

Xp 5,7367 11,0550 11,3500 13,5183 (T..) 10,415

dp -4,6783 0,6400 0,9350 3,1033 (X..)

SD 0,3165 0,1994 0,1026 0,3747

SE 0,3322 0,2093 0,1077 0,3933

Uji Aditivitas

W1 = (5,86)(-4,6783) + (11,13)(0,6400) + (11,27)(0,9350) +

(13,13)(3,1033)

= 30,9924

W2 = (6,27)(-4,6783) + (11,2)(0,6400) + (11,27)(0,9350) +

(13,67)(3,1033)

= 30,7949

W3 = (5,33)(-4,6783) + (11,2)(0,6400) + (11,4)(0,9350) + (14,13)(3,1033)

= 36,7416

W4 = (5,56)(-4,6783) + (11,2)(0,6400) + (11,53)(0,9350) +

(13,33)(3,1033)

= 33,3045

46


W5 = (5,67)(-4,6783) + (10,8)(0,6400) + (11,28)(0,9350) +

(13,65)(3,1033)

= 33,2932

W6 = (5,73)(-4,6783) + (10,8)(0,6400) + (11,35)(0,9350) + (13,2)(3,1033)

= 31,6814

N = � W. du

= (30,9924)(-0,0675)+ (30,7949)(0,1875) + (36,7416)(0,1000) +

(33,3045)(-0,0100) + (33,2932)(-0,0650) + (31,6814)(-0,1450)

= 0,2653

D = ��22 . dudp

= {(-4,6783)2 + (0,6400)2 + (0,9350)2 + (3,1033)2} x {(-0,0675)2 +

(0,1875)2 + (0,1000)2 + (-0,0100)2 + (-0,0650)2 + (-0,1450)2}

= 2,4621

FK = ( ) ( ) ==

24249,96.. 22

observasijumlahT

2603,3334


= {(5,86)2 + (11,13)2 + ... + (13,2)2} – 2603,3334

= 198,2620


Tp�2

- FK

= 2603,33346

)11,81(....)42,34( 22

−++

= 196,8078

47



Tu�2

- FK

= 2603,33344

)08,41(....)39,41( 22

−++

= 0,3003


= 198,2620– 196,8078– 0,3003

= 1,1539


N 2

= 2,4621

)2653,0( 2

= 0,0286


= 1,1539 – 0,0286

= 1,1253


Sumber Db JK KT FHitung Ftabel Ragam 5% 1%

Ulangan 5 0,3003 Perlakuan 3 196,8078 Galad acak 15 1,1539 Non aditivitas 1 0,0286 0,0286 0,3557 4,6 8,36 Sisa 14 1,1253 0,0804 F tabel 1 % > F tabel 5 % > F hitung � data aditif

48


DASIRA

Sumber Ragam Db JK KT FHitung Ftabel

5% 1% Ulangan 5 0,3003 0,0601 0,7806 2,56 4,36 Perlakuan 3 196,8078 65,6026 852,7818** 3,29 5,42 Galad acak 15 1,1539 0,0769 Total 23 198,2620



Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0769

Sx

= = 0,1132

W = 4,08 × 0,1132 = 0,4619

KV = −X..


= 415,10

0,0769x 100%

= 2,66 %

49


Purata Kadar Lemak Kasar Tempe (% ± SE) pada Perlakuan Variasi


U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

Purata ± SE 5,7367 ± 0,3322 11,0550 ± 0,2093 11,3500 ± 0,1077 13,5183 ± 0,3933

W=0,4619 (a) (b) (b) (c)




W = BNJ 5%

50

Lampiran 4. Data dan Contoh Penghitungan Asam Lemak Bebas


Asam Lemak Bebas


Belut Terhadap Kadar Asam Lemak Bebas

FFA (%) = %1001000

282

1

××

××W

NmlNaOH

Keterangan:

W1 = berat sampel

282 = berat molekul asam lemak linoleat

Contoh :

FFA (%) = %100100020

2821,09 ××

××

= 1,27%

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 1,27 1,90 1,55 1,41

2 1,29 1,87 1,53 1,37

3 1,27 1,87 1,51 1,39

4 1,27 1,89 1,53 1,41

5 1,27 1,87 1,51 1,39

6 1,29 1,87 1,55 1,39

51



Kadar Asam Lemak Bebas

Ulangan

Perlakuan

Tu Xu du U:B =

1:0

U:B =

1:1

U:B =

2:1

U:B =

3:1

1 1,27 1,90 1,55 1,41 6,13 1,5325 0,0129

2 1,29 1,87 1,53 1,37 6,06 1,5150 -0,0046

3 1,27 1,87 1,51 1,39 6,04 1,5100 -0,0096

4 1,27 1,89 1,53 1,41 6,1 1,5250 0,0054

5 1,27 1,87 1,51 1,39 6,04 1,5100 -0,0096

6 1,29 1,87 1,55 1,39 6,1 1,5250 0,0054

Tp 7,66 11,27 9,18 8,36 36,47

Xp 1,2767 1,8783 1,5300 1,3933 (T..) 1,5196

dp -0,2429 0,3588 0,0104 -0,1263 (X..)

SD 0,0103 0,0133 0,0179 0,0151 (�Tp2)

SE 0,0108 0,0140 0,0188 0,0158



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (1,27)(-0,2429) + (1,90)(0,3588) + (1,55)(0,0104) + (1,41)(-0,1263)

= 0,2113

W2 = (1,29)(-0,2429) + (1,87)(0,3588) + (1,53)(0,0104) + (1,37)(-0,1263)

= 0,2005

W3 = (1,27)(-2,2429) + (1,87)(0,3588) + (1,51)(0,0104) + (1,39)(-0,1263)

= 0,2026

W4 = (1,27)(-2,2429) + (1,89)(0,3588) + (1,53)(0,0104) + (1,41)(-0,1258)

= 0,2075

52


W5 = (1,27)(-2,2429) + (1,87)(0,3588) + (1,51)(0,0104) + (1,39)(-0,1263)

= 0,2026

W6 = (1,29)(-2,2429) + (1,87)(0,3575) + (1,55)(0,0104) + (1,39)(-0,1263)

= 0,1982

N = � W. du

= (0,2113)(0,0129) + (0,2005)(-0,0046) + (0,2026)(-0,0096) +

(0,2075)( 0,0054) + (0,2026)(-0,0096) + (0,1982)(0,0054)

= 0,0001

D = ��22 . dudp

= {(-0,2429)2 + (0,3588)2 + (0,0104)2 + (-0,1263)2} x {(0,0129)2 + (-

0,0046)2 + (-0,0096)2 + (0,0054)2 + (-0,0096)2 + (0,0054)2}

= 0,0001

FK = ( ) ( ) ==

2436,47.. 22

observasijumlahT

55,4192


= {(1,27)2 + (1,90)2 + ... + (1,39)2} – 55,4192

= 1,2267


Tp�2

- FK

= 55,41926

)36,8(....)66,7( 22

−++

= 1,2225

53



Tu�2

- FK

= 55,41924

)1,6(....)13,6( 22

−++

= 0,0017


= 1,2267– 1,2225– 0,0017

= 0,0024


N 2

= 0,0001

)-0,0001( 2

= 0,0001


= 0,0024– 0,0001

= 0,0023



Ragam 5% 1%

Ulangan 5 0,0017

Perlakuan 3 1,2225


Non aditivitas 1 0,0001 0,0001 1,0000 4,6 8,36

Sisa 14 0,0023 0,0001


54


DASIRA

Sumber

Ragam Db JK KT FHitung

Ftabel

5% 1%

Ulangan 5 0,0017 0,0003 2,1252 2,56 4,36

Perlakuan 3 1,2225 0,4075 2516,3894** 3,29 5,42

Galad acak 15 0,0024 0,0002

Total 23 1,2267



Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0002

Sx

= = 0,0052

W = 4,08 × 0,0052= 0,2122

KV = −X..


= 5196.1

0,0002x 100%

= 0,93 %

55


Purata Kadar Asam Lemak Bebas Tempe (% ± SE) pada Perlakuan Variasi


U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

Purata ± SE 1,2767 ± 0,0108 1,8783 ± 0,0140 1,5300 ± 0,0188 1,3933 ± 0,0158

W=0,02122 (a) (d) (c) (b)




W = BNJ 5%


Asam Lemak Bebas

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 1,27 1,90 2,40 2,85

2 1,29 1,87 2,41 2,82

3 1,27 1,87 2,43 2,80

4 1,27 1,89 2,42 2,82

5 1,27 1,87 2,42 2,84

6 1,29 1,87 2,42 2,81

56



Kadar Asam Lemak Bebas

Ulangan

Perlakuan Tu Xu du

U:B =

1:0

U:B =

1:1

U:B =

1:2

U:B =

1:3

1 1,27 1,90 2,40 2,85 8,42 2,1050 0,0062

2 1,29 1,87 2,41 2,82 8,39 2,0975 -0,0013

3 1,27 1,87 2,43 2,80 8,37 2,0925 -0,0063

4 1,27 1,89 2,42 2,82 8,4 2,1000 0,0012

5 1,27 1,87 2,42 2,84 8,4 2,1000 0,0012

6 1,29 1,87 2,42 2,81 8,39 2,0975 -0,0013

Tp 7,66 11,27 14,5 16,94 50,37

Xp 1,2767 1,8783 2,4167 2,8233 (T..) 2,0987

dp -0,8221 -0,2204 0,3179 0,7246 (X..)

SD 0,0103 0,0133 0,0103 0,0186

SE 0,0108 0,0140 0,0108 0,0195



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (1,27)(-0,8221) + (1,90)(-0,2204) + (2,40)(0,3179) + (2,85)(0,7246)

= 1,3652

W2 = (1,29)(-0,8221) + (1,87)(-0,2204) + (2,41)(0,3179) + (2,82)(0,7246)

= 1,3368

W3 = (1,27)(-0,8221) + (1,87)(-0,2204) + (2,43)(0,3179) + (2,80)(0,7246)

= 1,3451

W4 = (1,27)(-0,8221) + (1,89)(-0,2204) + (2,42)(0,3179) + (2,82)(0,7246)

= 1,3520

57


W5 = (1,27)(-0,8221) + (1,87)(-0,2204) + (2,42)(0,3179) + (2,84)(0,7246)

= 1,3709

W6 = (1,29)(-0,8221) + (1,87)(-0,2204) + (2,42)(0,3179) + (2,81)(0,7246)

= 1,3328

N = � W. du

= (1,3652)(0,0062) + (1,3368)(-0,0013) + (1,3451)(-0,0063) +

(1,3520)(0,0012) + (1,3709)(0,0012) + (1,3328)(-0,0013)

= 0,0002

D = ��22 . dudp

= {(-0,8221)2 + (-0,2204)2 + (0,3179)2 + (0,7246)2} x {(0,0062)2 + (-

0,0013)2 + (-0,0063)2 + (0,0012)2 + (0,0012)2 + (-0,0013)2}

= 0,0001

FK = ( ) ( ) ==

2450,37.. 22

observasijumlahT

105,7140


= {(1,27)2 + (1,90)2 + ... + (2,81)2} – 105,7140

= 8,1067


Tp�2

- FK

= 105,71406

)94,16(....)66,7( 22

−++

= 8,1030

58



Tu�2

- FK

= 105,71404

)39,8(....)42,8( 22

−++

= 0,0017


= 1,2267– 1,2225– 0,0017

= 0,0033


N 2

= 0,0001

)-0,0002( 2

= 0,0003


= 0,0033– 0,0003

= 0,0030



Ragam 5% 1%

Ulangan 5 0,0003

Perlakuan 3 8,1030


Non

aditivitas 1 0,0003 0,0003 1,5026 4,6 8,36

Sisa 14 0,0030 0,0002


59


DASIRA

Sumber


Ftabel

5% 1%

Ulangan 5 0,0003 0,0001 0,3026 2,56 4,36

Perlakuan 3 8,1030 2,7010 12109,0598** 3,29 5,42

Galad acak 15 0,0033 0,0002

Total 23 8,1067



Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0002

Sx

= = 0,0061

W = 4,08 × 0,0061= 0,0249

KV = −X..


= 0987,2

0,0002x 100%

= 0,67 %

60


Purata Kadar Asam Lemak Bebas Tempe (% ± SE) pada Perlakuan Variasi


U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

Purata ± SE 1,2767 ± 0,0108 1,8783 ± 0,0140 2,4167 ± 0,0108 2,8233 ± 0,0195

W=0,0249 (a) (b) (c) (d)




W = BNJ 5%

61

Lampiran 5. Data dan Contoh Penghitungan Kadar Abu


Abu

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 1,03 1,33 1,15 1,06

2 1,02 1,35 1,17 1,06

3 1,04 1,39 1,26 1,06

4 1,04 1,43 1,27 1,06

5 1,02 1,46 1,28 1,08

6 1,04 1,46 1,31 1,08


Belut Terhadap Kadar Abu

Kadar Abu (%) = %1001

02 ×−

WWW

Keterangan:

W0= berat cawan porselin


W2= berat cawan porselin dan abu

Contoh :

Kadar Abu (%) = %1000056,1

8191,328295,32 ×−

= 1,03%

62



Kadar Abu

Ulangan

Perlakuan

Tu Xu du R:B =

1:0

R:B =

1:1

R:B =

2:1

R:B =

3:1

1 1,03 1,33 1,15 1,06 4,57 1,1425 -0,0429

2 1,02 1,35 1,17 1,06 4,6 1,1500 -0,0354

3 1,04 1,39 1,26 1,06 4,75 1,1875 0,0021

4 1,04 1,43 1,27 1,06 4,8 1,2000 0,0146

5 1,02 1,46 1,28 1,08 4,84 1,2100 0,0246

6 1,04 1,46 1,31 1,08 4,89 1,2225 0,0371

Tp 6,19 8,42 7,44 6,4 28,45

Xp 1,0317 1,4033 1,2400 1,0667 (T..) 1,1854

dp -0,1538 0,2179 0,0546 -0,1188 (X..)

SD 0,0098 0,0557 0,0645 0,0103

SE 0,0103 0,0585 0,0677 0,0108



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (1,03)(-0,1538) + (1,33)(0,2179) + (1,15)(0,0546) + (1,06)(-0,1188)

= 0,0684

W2 = (1,02)(-0,1538) + (1,35)(0,2179) + (1,17)(0,0546) + (1,06)(-0,1188)

= 0,0754

W3 = (1,04)(-0,1538) + (1,39)(0,2179) + (1,26)(0,0546) + (1,06)(-0,1188)

= 0,0859

W4 = (1,04)(-0,1538) + (1,43)(0,2179) + (1,27)(0,0546) + (1,06)(-0,1188)

= 0,0952

W5 = (1,02)(-0,1538) + (1,46)(0,2179) + (1,28)(0,0546) + (1,08)(-0,1188)

= 0,1030

63


W6 = (1,04)(-0,1538) + (1,46)(0,2179) + (1,31)(0,0546) + (1,08)(-0,1188)

= 0,1015

N = � W. du

= (0,0684)(-0,0429) + (0,0754)(-0,0354) + (0,0859)(0,0021) +

(0,0952)( 0,0146) + (0,1030)(0,0246) + (0,1015)(0,0371)

= 0,0023

D = ��22 . dudp

= {(-0,1538)2 + (0,2179)2 + (0,0546)2 + (-0,1188)2} x {(-0,0429)2 + (-

0,0354)2 + (0,0021)2 + (0,0146)2 + (0,0246)2 + (0,0371)2}

= 0,0005

FK = ( ) ( ) ==

2428,45.. 22

observasijumlahT

33,7251


= {(1,03)2 + (1,33)2 + ... + (1,08)2} – 33,7251

= 0,5666


Tp�2

- FK

= 33,72516

)4,6(....)19,6( 22

−++

= 0,5292

64



Tu�2

- FK

= 33,72514

)89,4(....)57,4( 22

−++

= 0,0212


= 0,5666– 0,5292– 0,0212

= 0,0162


N 2

= 0,0005

)0023,0( 2

= 0,0109


= 0,0162– 0,0109

= 0,0052



Ragam 5% 1%

Ulangan 5 0,0212

Perlakuan 3 0,5292


Non aditivitas 1 0,0109 0,0109 29,2012 4,6 8,36

Sisa 14 0,0052 0,0004



65



U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 1,03 1,33 1,25 1,06 4,67 1,1675

2 1,02 1,35 1,25 1,06 4,68 1,1700

3 1,04 1,39 1,26 1,06 4,75 1,1875

4 1,04 1,43 1,27 1,06 4,8 1,2000

5 1,02 1,38 1,28 1,08 4,76 1,1900

6 1,04 1,4 1,31 1,08 4,83 1,2075

Tp 6,19 8,28 7,62 6,4 28,49 1,1871

(T..) (X..)

FK = ( ) ( ) ==

2428,49.. 22

observasijumlahT

33,8200


= {(1,03)2 + (1,33)2 + ... + (1,08)2} – 33,8200

= 0,5065


Tp�2

- FK

= 33,82006

)4,6(....)19,6( 22

−++

= 0,4965


Tu�2

- FK

= 33,82004

)83,4(....)67,4( 22

−++

= 0,0051

66



= 0,5065– 0,4965– 0,0051

= 0,0049

DASIRA


Ulangan 5 0,0051 0,0010 3,0758* 2,56 4,36 Perlakuan 3 0,4965 0,1655 501,9166** 3,29 5,42 Galad acak 15 0,0049 0,0003 Total 23 0,5065

Ftabel 5% > Fhitung ulangan > Ftabel 1% � Antar ulangan berbeda nyata


Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0003

Sx

= = 0,0074

W = 4,08 × 0,0074 = 0,0302

KV = −X..


= 1871,1

0,0003x 100%

= 1,46 %

67


Purata Kadar Abu Tempe (% ± SE) pada Perlakuan Variasi Penambahan

Usar Tempe

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

Purata ± SE 1,0317 ± 0,0103 1,3800 ± 0,0376 1,2700 ± 0,0239 1,0667 ± 0,0108

W=0,0302 (a) (d) (c) (b)

Keterangan: Angka-angka yang diikuti oleh huruf yang sama menunjukkan antar perlakuan tidak berbeda



W = BNJ 5%


Abu

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 1,03 1,33 1,47 1,74

2 1,02 1,35 1,54 1,61

3 1,04 1,39 1,56 1,8

4 1,04 1,43 1,56 1,83

5 1,02 1,46 1,56 1,67

6 1,04 1,46 1,57 1,76

68



Kadar Abu

Ulangan

Perlakuan

Tu Xu du R:B =

1:0

R:B =

1:1

R:B =

1:2

R:B =

1:3

1 1,03 1,33 1,47 1,74 5,57 1,3925 -0,0358

2 1,02 1,35 1,54 1,61 5,52 1,3800 -0,0483

3 1,04 1,39 1,56 1,8 5,79 1,4475 0,0192

4 1,04 1,43 1,56 1,83 5,86 1,4650 0,0367

5 1,02 1,46 1,56 1,67 5,71 1,4275 -0,0008

6 1,04 1,46 1,57 1,76 5,83 1,4575 0,0292

Tp 6,19 8,42 9,26 10,41 34,28

Xp 1,0317 1,4033 1,5433 1,7350 (T..) 1,4283

dp -0,3967 -0,0250 0,1150 0,3067 (X..)

SD 0,0098 0,0557 0,0372 0,0822

SE 0,0103 0,0585 0,0391 0,0862



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2608

W2 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2325

W3 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2841

W4 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2923

W5 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2504

69


W6 = (1,03)(-0,3967) + (1,33)(-0,0250) + (1,47)(0,1150) + (1,74)(0,3067)

= 0,2713

N = � W. du

= (0,2608)(-0,0358) + (0,2325)(-0,0483) + (0,2841)(0,0192) +

(0,2923)( 0,0367) + (0,2923)(-0,0008) + (0,2713)(0,0292)

= 0,0033

D = ��22 . dudp

= {(-0,3967)2 + (-0,0250)2 + (0,1150)2 + (0,3067)2} x {(-0,0358)2 + (-

0,0483)2 + (0,0192)2 + (0,0367)2 + (-0,0008)2 + (0,0292)2}

= 0,0016

FK = ( ) ( ) ==

2434,28.. 22

observasijumlahT

48,9633


= {(1,03)2 + (1,33)2 + ,,, + (1,76)2} – 48,9633

= 1,6481


Tp�2

- FK

= 48,96336

)41,10(....19,6( 22

−++

= 1,5914


Tu�2

- FK

= 48,96334

)83,5(....)57,5( 22

−++

= 0,0247

70



= 1,6481– 1,5914– 0,0247

= 0,0320


N 2

= 0,0016

)0033,0( 2

= 0,0066


= 0,0320– 0,0066

= 0,0254



Ragam 5% 1%

Ulangan 5 0,0247

Perlakuan 3 1,5914


Non

aditivitas 1 0,0066 0,0066 3,6240 4,6 8,36

Sisa 14 0,0254 0,0018

F tabel 1 % < F tabel 5 % < F hitung � data aditif

71


DASIRA

Sumber


Ftabel

5% 1%

Ulangan 5 0,0247 0,0049 2,3212 2,56 4,36

Perlakuan 3 1,5914 0,5305 248,9208** 3,29 5,42

Galad acak 15 0,0320 0,0021

Total 23 1,6481



Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,0021

Sx

= = 0,0188

W = 4,08 × 0,0188 = 0,0767

KV = −X..


= 4283,1

0,0021x 100%

= 3,21 %

72


Purata Kadar Abu Tempe (% ± SE) pada Perlakuan Variasi Penambahan

Tepung Belut

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

Purata ± SE 1,0317 ± 0,0103 1,4033 ± 0,0585 1,5433 ± 0,0391 1,7350 ± 0,0862

W=0,0767 (a) (b) (c) (d)




W = BNJ 5%

73

Lampiran 6. Data dan Contoh Penghitungan Kadar Air

� Data Pengaruh Variasi Penambahan Usar Tempe Terhadap Kadar Air

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 41,08 58,72 59,41 61,52

2 40,69 58,75 60,15 62,02

3 41,23 58,88 60,15 62,12

4 40,75 59,02 60,2 62,16

5 40,68 59,17 60,38 63,63

6 40,75 59,38 60,91 65,63


Belut Terhadap Kadar Air

Kadar Air (%) = %100)(

1

21 ×−

WWW

Keterangan:


W2= berat sampel kering

Contoh :

Kadar Air (%) = %1000034,1

)5912,00034,1( ×−

= 41,08%

74



Kadar Air

Ulangan Perlakuan

Tu Xu du R:B = 1:0 R:B = 1:1 R:B = 2:1 R:B = 3:1

1 41,08 58,72 59,41 61,52 220,73 55,1825 -0,5417

2 40,69 58,75 60,15 62,02 221,61 55,4025 -0,3217

3 41,23 58,88 60,15 62,12 222,38 55,5950 -0,1292

4 40,75 59,02 60,2 62,16 222,13 55,5325 -0,1917

5 40,68 59,17 60,38 63,63 223,86 55,9650 0,2408

6 40,75 59,38 60,91 65,63 226,67 56,6675 0,9433

Tp 245,18 353,92 361,2 377,08 1337,38

Xp 40,8633 58,9867 60,2000 62,8467 (T..) 55,72417

dp -14,8608 3,2625 4,4758 7,1225 (X..)

SD 0,2327 0,2561 0,4828 1,5365

SE 0,2442 0,2688 0,5068 1,6127



dp = X p - X .. ; du = X u - X ..

Uji Aditivitas

W1 = (41,08)(-14,8608) + (58,72)(3,2625) + (59,41)(4,4758) +

(61,52)(7,1225)

= 285,1764

W2 = (40, 69)(-14,8608) + (58,75)(3,2625) + (60,15)(4,4758) +

(62,02)(7,1225)

= 297,9434

W3 = (41,23)(-14,8608) + (58,88)(3,2625) + (60,15)(4,4758) +

(62,12)(7,1225)

= 291,0549

W4 = (40,75)(-14,8608) + (59,02)(3,2625) + (60,20)(4,4758) +

(62,16)(7,1225)

= 299,1536

75


W5 = (40,68)(-14,8608) + (59,17)(3,2625) + (60,38)(4,4758) +

(63,63)(7,1225)

= 311,9589

W6 = (40,75)(-14,8608) + (59,38)(3,2625) + (60,91)(4,4758) +

(65,63)(7,1225)

= 328,2210

N = � W. du

= (285,1764)(-0,5417) + (297,9434)(-0,3217) + (291,0549)(-0,1292) +

(299,1536)(-0,1917) + (311,9589)(0,2408) + (328,2210)(0,9433)

= 39,5105

D = ��22 . dudp

= {(-14,8608)2 + (3,2625)2 + (4,4758)2 + (7,1225)2} x {(-0,5417)2 +(-

0,3217)2 + (-0,1292)2 + (-0,1917)2 + (0,2408)2 + (0,9433)2}

= 422,5990

FK = ( ) ==

24)38,337(1.. 22

observasijumlahT

74524,3860


= {(41,08)2 + (58,72)2 + ,,, + (65,63)2} – 74524,3860

= 1827,0760


Tp�2

- FK

= 74524,38606

)377,08(....)245,18( 22

−++

= 1813,5082

76



Tu�2

- FK

= 74524,38604

)226,67(....)220,73( 22

−++

= 5,5927


= 1827,0760– 1813,5082– 5,5927

= 7,9751


N 2

= 422,5990

)39,5105( 2

= 3,6940


= 7,9751– 3,6940

= 4,2811



Ragam 5% 1%

Ulangan 5 5,5927

Perlakuan 3 1813,5082


Non

aditivitas 1 3,6940 3,6940 12,0800 4,6 8,36

Sisa 14 4,2811 0,3058


77




U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

1 41,08 58,72 60,01 61,52 221,33 55,3325

2 40,69 58,75 60,15 62,02 221,61 55,4025

3 41,23 58,88 60,15 62,12 222,38 55,5950

4 40,75 59,02 60,2 62,16 222,13 55,5325

5 40,68 59,17 60,38 63,63 223,86 55,9650

6 40,75 59,38 60,91 62,65 223,69 55,9225

Tp 245,18 353,92 361,8 374,1 1335 55,625

(T..) (X..)

FK = ( ) ( ) ==

241335.. 22

observasijumlahT

74259,375


= {(41,08)2 + (58,72)2 + ... + (62,65)2} – 74259,375

= 1781,4646


Tp�2

- FK

= 74259,3756

)374,1(....)245,18( 22

−++

= 1777,7331


Tu�2

- FK

= 74259,3754

)223,69(....)221,33( 22

−++

= 1,3945

78



= 1781,4646– 1777,7331– 1,3945

= 2,3370

DASIRA


Ragam 5% 1%

Ulangan 5 1,3945 0,2789 1,7901 2,56 4,36

Perlakuan 3 1777,7331 592,5777 3803,5055** 3,29 5,42

Galad acak 15 2,3370 0,1558

Total 23 1781,4646



Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,1558

Sx

= = 0,1611

W = 4,08 × 0,1611 = 0,6573

KV = −X..


= 55,6250,1558

x 100%

= 0,71 %

79


Purata Kadar Air Tempe (% ± SE) pada Perlakuan Variasi Penambahan

Usar Tempe

U:B = 1:0 U:B = 1:1 U:B = 2:1 U:B = 3:1

Purata ± SE 40,8633 ± 0,2442 58,9867 ± 0,2688 60,3000 ± 0,3377 62,3500 ± 0,7591

W=0,6573 (a) (b) (c) (d)




W = BNJ 5%



Air

Ulangan Perlakuan

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

1 41,08 58,72 58,3 53,88

2 40,69 58,75 58,37 57,8

3 41,23 58,88 58,43 58,12

4 40,75 59,02 58,59 58,21

5 40,68 59,17 58,59 58,24

6 40,75 59,38 58,67 58,16

80


Kadar Air

Ulangan

Perlakuan Tu Xu du

U:B =

1:0

U:B =

1:1

U:B =

1:2

U:B =

1:3

1 41,08 58,72 58,3 53,88 211,98 52,9950 -0,9408

2 40,69 58,75 58,37 57,8 215,61 53,9025 -0,0333

3 41,23 58,88 58,43 58,12 216,66 54,1650 0,2292

4 40,75 59,02 58,59 58,21 216,57 54,1425 0,2067

5 40,68 59,17 58,59 58,24 216,68 54,1700 0,2342

6 40,75 59,38 58,67 58,16 216,96 54,2400 0,3042

Tp 245,18 353,92 350,95 344,41 1294,46

Xp 40,8633 58,9867 58,4917 57,4017 (T..) 53,9358

dp -13,0725 5,0508 4,5558 3,4658 (X..)

SD 0,2327 0,2561 0,1459 1,7325

SE 0,2442 0,2688 0,1532 1,8185

Uji Aditivitas

W1 = (41,08)(-13,0725) + (58,72)(5,0508) + (58,3)(4,5558) +

(53,88)(3,4658)

= 211,9108

W2 = (40,69)(-13,0725) + (58,75)(5,0508) + (58,37)(4,5558) +

(57,8)(3,4658)

= 231,0656

W3 = (41,23)(-13,0725) + (58,88)(5,0508) + (58,43)(4,5558) +

(58,12)(3,4658)

= 226,0455

W4 = (40,75)(-13,0725) + (59,02)(5,0508) + (58,59)(4,5558) +

(58,21)(3,4658)

= 234,0682

81


W5 = (40,68)(-13,0725) + (59,17)(5,0508) + (58,59)(4,5558) +

(58,24)(3,4658)

= 235,8449

W6 = (40,75)(-13,0725) + (59,38)(5,0508) + (58,67)(4,5558) +

(58,16)(3,4658)

= 236,0777

N = � W. du

= (211,9108)(-0,9408)+ (231,0656)( -0,0333) + (226,0455)( 0,2292) +

(234,0682)(0,2067) + (235,8449)(0,2342) + (236,0777)(0,3042)

= 20,1352

D = ��22 . dudp

= {(-13,0725)2 + (5,0508)2 + (4,5558)2 + (3,4658)2} x {(-0,9408)2 + -

0,0333)2 + (0,2292)2 + (0,2067)2 + (0,2342)2 + (0,3042)2}

= 258,6991

FK = ( ) ( ) ==

241294,46.. 22

observasijumlahT

69817,7788


= {(41,08)2 + (58,72)2 + ... + (58,16)2} – 69817,7788

= 1390,7260


Tp�2

- FK

= 69817,77886

)344,41(....)245,18( 22

−++

= 1375,0127

82



Tu�2

- FK

= 69817,77884

)216,96(....)211,98( 22

−++

= 4,5154


= 1390,7260– 1375,0127– 4,5154

= 11,1978


N 2

= 258,6991

)20,1352( 2

= 1,5672


= 11,1978 – 1,5672

= 9,6306



Ulangan 5 4,5154 Perlakuan 3 1375,0127 Galad acak 15 11,1978 Non aditivitas 1 1,5672 1,5672 2,2782 4,6 8,36 Sisa 14 9,6306 0,6879

F tabel 1 % < F tabel 5 % < F hitung � data aditif

83


DASIRA

Sumber Ragam Db JK KT FHitung Ftabel

5% 1% Ulangan 5 4,5154 0,9031 1,2097 2,56 4,36 Perlakuan 3 1375,0127 458,3376 613,9656** 3,29 5,42 Galad acak 15 11,1978 0,7465 Total 23 1390,7260 Ftabel 5% > Fhitung ulangan > Ftabel 1% � Antar ulangan tidak berbeda nyata


Uji BNJ 5%

W = q (p,n2) xS

nAcak Galad KT

Sx

=

60,7465

Sx

= = 0,3527

W = 4,08 × 0,3527= 1,439

KV = −X..


= 53,9358

0,7465x 100%

= 1,60 %

84


Purata Kadar Air Tempe (% ± SE) pada Perlakuan Variasi Penambahan

Tepung Belut

U:B = 1:0 U:B = 1:1 U:B = 1:2 U:B = 1:3

Purata ± SE 40,8633 ± 0,2442 58,9867 ± 0,2688 58,4917 ± 0,1532 57,4017 ± 1,8185

W=0,3527 (a) (c) (bc) (b)




W = BNJ 5%

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