Topik Bentuk Tugas Mahasiswa Jumlah Pertemuan Dosen

I. Bioteknologi Pertanian meliputi Sejarah Singkat, Pengertian Dasar dan Ruang Lingkup

Ceramah dan diskusi

  1 Agro

II. Teori Dasar Bioteknologi (Bahan Genetik, Replikasi DNA, Ekspresi Genetik dan DNA Rekombinan)

Ceramah dan diskusi

  3 Agro

III. Teknik-Teknik Dasar bioteknologi (teknik pada tingkat protein dan DNA)

Ceramah dan diskusi

  3 Agro (1)HPT (2)

IV. Evaluasi I     1 TimV. Bioteknologi

Konvensional dan Modern

Ceramah dan diskusi

  2 HPT

VI. Bioteknologi Pemuliaan Tanaman

Diskusi kelompok

Telaah Jurnal

1 Agro+HPT (Tim Kelas)

VII. Bioteknologi Tanah 

Diskusi kelompok

Telaah Jurnal

1 Agro+HPT(Tim Kelas)

VIII Bioteknologi Perlindungan Tanaman

Diskusi kelompok

Telaah Jurnal

1 Agro+HPT(Tim Kelas)

IX Regulasi Tanaman Produk rekayasa genetik

Ceramah dan diskusi

  2 Agro (1)HPT (1) 

X Evaluasi II     1 Tim

KONTRAK KULIAH BIOTEKNOLOGI

  Acara Jadwal (Minggu ke)

Penanggung Jawab

I Isolasi/ekstraksi dan pengukuran Kandungan Protein

1 Prof. Tri Agus Siswoyo

II Pola Protein pada Tanaman ( Elektroforesis) 2 Prof. Tri Agus Siswoyo

III Isolasi/ekstrasi DNA (Plasmid) dan pengukuran Kandungan DNA/plasmid

3 Prof. Tri Agus Siswoyo

IV Elektrophoresis dan Visualisasi DNA 4 Prof. Tri Agus Siswoyo

V Amplifikasi DNA (PCR) 5 Prof. Tri Agus Siswoyo

VI Elektrophoresis dan Visualisasi PCR Produk 6 Prof. Tri Agus Siswoyo

VII Evaluasi 7 Prof. Tri Agus Siswoyo

INTRODUCTION TO PLANT BIOTECHNOLOGY

Biotechnology ?Biotechnology ?

The application of biological organisms, systems The application of biological organisms, systems or processes to manufacturing and service or processes to manufacturing and service industriesindustries

The integrated use of biochemistry, microbiology The integrated use of biochemistry, microbiology and engineering sciences in order to achieve and engineering sciences in order to achieve technological (industrial) application capabilities technological (industrial) application capabilities of microorganism, cultured tissue cells and part of microorganism, cultured tissue cells and part thereofthereof

A technology using biological phenomena by A technology using biological phenomena by copying and manufacturing various kinds of copying and manufacturing various kinds of useful substanceuseful substance

The application of scientific and engineering The application of scientific and engineering principles to the processing of materials by principles to the processing of materials by biological-agents to provide goods and servicesbiological-agents to provide goods and services

Biotechnology ?Biotechnology ?

The science of the production processes based on The science of the production processes based on the action of microorganisms and their active the action of microorganisms and their active components and production processes involving the components and production processes involving the use of cells and tissues from higher organisms. It use of cells and tissues from higher organisms. It was not included medical technology, agriculture was not included medical technology, agriculture and traditional crop breedingand traditional crop breeding

The use of living organism and their component in The use of living organism and their component in agriculture, food and other industrial processesagriculture, food and other industrial processes

The use of microbial, animal and plant cells or The use of microbial, animal and plant cells or enzymes to synthesize, breakdown and transform enzymes to synthesize, breakdown and transform materialsmaterials

The integration of natural sciences and organisms, The integration of natural sciences and organisms, cells, parts thereof and molecular analogues to cells, parts thereof and molecular analogues to product and servicesproduct and services

Stages of Biotechnology Stages of Biotechnology DevelopmentDevelopment

Ancient biotechnology Ancient biotechnology early history as related to food and shelter; early history as related to food and shelter; Includes domesticationIncludes domestication

Classical biotechnology Classical biotechnology built on ancient biotechnology; Fermentation built on ancient biotechnology; Fermentation promoted food production, and medicinepromoted food production, and medicine

Modern biotechnologyModern biotechnologymanipulates genetic information in organism; manipulates genetic information in organism; Genetic engineeringGenetic engineering

Traditional/old biotechnologyTraditional/old biotechnology

The conventional techniques that have been used to The conventional techniques that have been used to produce beer, wine, cheese, many other foodproduce beer, wine, cheese, many other food

New/modern biotechnologyNew/modern biotechnology

All methods of genetic modification by recombinant All methods of genetic modification by recombinant DNA and cell fusion techniques, together with the DNA and cell fusion techniques, together with the modern development of traditional biotechnological modern development of traditional biotechnological processprocess

Stages of Biotechnology Stages of Biotechnology DevelopmentDevelopment

Areas of BiotechnologyAreas of Biotechnology Organismic biotechnologyOrganismic biotechnology

uses intact organisms; Does not uses intact organisms; Does not alter genetic materialalter genetic material

Molecular biotechnologyMolecular biotechnologyalters genetic makeup to achieve alters genetic makeup to achieve specific goalsspecific goals• Transgenic organism- an organism Transgenic organism- an organism

with artificially altered genetic materialwith artificially altered genetic material

Biotechnology: Biotechnology: A collection of technologiesA collection of technologies

The Applications of The Applications of BiotechnologyBiotechnology

Medical BiotechnologyMedical Biotechnology DiagnosticsDiagnostics TherapeuticsTherapeutics VaccinesVaccines

Agricultural BiotechnologyAgricultural Biotechnology Plant agriculturePlant agriculture Animal agricultureAnimal agriculture Food processingFood processing

Environmental BiotechnologyEnvironmental Biotechnology Cleaning through bioremediationCleaning through bioremediation Preventing environmental problemsPreventing environmental problems Monitoring the environmentMonitoring the environment

Plant agriculturePlant agriculture

Crop production and protectionCrop production and protection• Genetically engineered (transgenic) cropsGenetically engineered (transgenic) crops• Using biological methods to protect cropsUsing biological methods to protect crops

• Exploiting cooperative relationships in natureExploiting cooperative relationships in nature

Nutritional value of cropsNutritional value of crops• Improving food quality and safetyImproving food quality and safety

• Healthier cooking oils by decreasing the concentration of Healthier cooking oils by decreasing the concentration of saturated fatty acids in vegetable oilssaturated fatty acids in vegetable oils

• Functional foodsFunctional foodsFoods containing significant levels of biologically active Foods containing significant levels of biologically active

components that impart health benefitscomponents that impart health benefits

Plant Biotechnology

TRADITIONAL PLANT BIOTECHNOLOGY

breeding

tissue culture

inter-specific hybridisation

mapping phenotypic/biochemical markers

Now use a molecular approach to manipulation: molecular markers & mapping

gene cloning

plant transformation

PLANT BIOTECHNOLOGY

a process to produce a genetically modified a process to produce a genetically modified plant by removing genetic information from plant by removing genetic information from

an organism, manipulating it in the an organism, manipulating it in the laboratory and then transferring it into a laboratory and then transferring it into a

plant to change certain of its characteristics plant to change certain of its characteristics ..

Exogenous genes (non-plant

genes)

Applications:

transgenic

pathogen-derived genes

bacterial genes

any other organism

Pathogen resistance

Herbicide resistance

bioreactors

Delivery systems

Endogenous genes (Plant

genes)

Gene discovery (functional genomics)

Applications:

markers

transgenic

Enzymes in biochemical pathway

Natural resistance genes

Marker assisted breeding

Plant improvement

Mapping

ESTs, libraries

Silencing, expressionMutants, arrays