Gambar lampiran 1. Jenis leukosit. a. Monosit, b. Basofil ...

56

Gambar lampiran 1. Jenis leukosit. a. Monosit, b. Basofil, c.

Eosinofil, d. Netrofil, e. Limfosit dengan

perbesaran mikroskop 1600X

a

b d

e

c

57

Alur Kerja

A. Perhitungan Jumlah Total Leukosit

Tahap I Tahap II

(Pengambilan sampel darah) (Sampel dengan Pipet Leukosit)

Tahap III Tahap IV

(Pencampuran Sampel dengan (Homogenisasi Darah dan Turk)

Larutan Turk)

58

Tahap V Tahap VI

(Peletakan Sampel (Pengamatan dengan Mikroskop)

pada Haemocitometer)

Gambar lampiran 2. Alur kerja pembuatan preparat untuk

menghitung jumlah total leukosit

B. Jenis Leukosit

Tahap I Tahap II

(Pengambilan Sampel Darah) (Sampel darah Pada Kaca Preparat)

59

Tahap III Tahap IV

(Pembuatan Darah Apus) (Sampel Preparat)

Tahap V Tahap VI

(Pewarnssn) (Pemberia Larutan Buffer)

Tahap VII Tahap VIII

(Pembilasan dengan Aquadest) (PengamatanMikroskop

Perbesaran 1600x)

Gambar lampiran 3. Alur kerja pembuatan preparat untuk

menghitung jenis-jenis leukosit

60

Lampiran 4. Jumlah Total Leukosit

Tests of Normality

Perlakuan

Kolmogorov-Smirnova Shapiro-Wilk

Statisti

c df Sig.

Statisti

c df Sig.

Jumlah Total

Leukosit

Vitamin E

tokotrienol .269 4 . .823 4 .150

Vitamin E

tokotrienol +

Asam

ascorbat

.273 4 . .864 4 .277

Kontrol .263 4 . .866 4 .283

a. Lilliefors Significance

Correction

Uji RAL menggunakan Uji OneWay Anova

Between-Subjects Factors

Value Label N

Perlakuan 1 Vitamin E

tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4

61

Descriptive Statistics

Dependent Variable:Jumlah Total Leukosit

Perlakuan Mean

Std.

Deviation N

Vitamin E tokotrienol 6783.25 2484.012 4

Vitamin E tokotrienol +

Asam ascorbat 7916.25 3725.932 4

Kontrol 5045.00 16.793 4

Total 6581.50 2643.893 12

Levene's Test of Equality of Error

Variancesa

Dependent Variable:Jumlah Total

Leukosit

F df1 df2 Sig.

4.736 2 9 .039

Tests the null hypothesis that the error

variance of the dependent variable is

equal across groups.

a. Design: Perlakuan

Tests of Between-Subjects Effects


Source

Type III Sum

of Squares df Mean Square F Sig.

Model 5.365E8a 3 1.788E8 26.755 .000

Perlakuan 5.365E8 3 1.788E8 26.755 .000

Error 6.016E7 9 6684388.389

Total 5.967E8 12

a. R Squared = .899 (Adjusted R Squared = .866)

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Post Hoc Tests

Multiple Comparisons

Jumlah Total

Leukosit

Games-Howell

(I) Perlakuan

(J)

Perlakuan

Mean

Difference

(I-J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam

ascorbat

-1133.00 2239.022 .872 -8306.89 6040.89

Kontrol 1738.25 1242.034 .444 -3451.55 6928.05

Vitamin E

tokotrienol +

Asam ascorbat

Vitamin E

tokotrienol 1133.00 2239.022 .872 -6040.89 8306.89

Kontrol 2871.25 1862.985 .391 -4913.43 10655.93

Kontrol Vitamin E

tokotrienol -1738.25 1242.034 .444 -6928.05 3451.55

Vitamin E

tokotrienol +

Asam

ascorbat

-2871.25 1862.985 .391 -10655.93 4913.43

Based on observed means.

The error term is Mean Square(Error)

= 6684388.389.

63

Estimated Marginal Means

Perlakuan


Perlakuan Mean Std. Error

95% Confidence Interval

Lower Bound Upper Bound

Vitamin E tokotrienol 6.783E3 1292.709 3858.939 9707.561


Asam ascorbat 7.916E3 1292.709 4991.939 10840.561

Kontrol 5.045E3 1292.709 2120.689 7969.311

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Lampiran 5. Jenis-Jenis Leukosit

NEUTROFIL

Tests of Normality

Perlakuan


Statisti

c df Sig.

Statisti

c df Sig.

Neutrof

il

Vitamin E

tokotrienol .318 4 . .843 4 .205

Vitamin E

tokotrienol + Asam

ascorbat

.355 4 . .773 4 .062

Kontrol .333 4 . .783 4 .075

a. Lilliefors Significance Correction



Value Label N


tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4


65

Dependent Variable:Neutrofil

Perlakuan Mean

Std.

Deviation N



Asam ascorbat 50.8325 15.66604 4

Kontrol 34.0850 4.36535 4

Total 44.3892 13.63859 12


Variancesa


F df1 df2 Sig.

1.855 2 9 .212







Source

Type III Sum


Model 24295.171a 3 8098.390 52.220 .000

Perlakuan 24295.171 3 8098.390 52.220 .000

Error 1395.729 9 155.081

Total 25690.900 12


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Perlakuan





Vitamin E tokotrienol 48.250 6.227 34.165 62.335


Asam ascorbat 50.832 6.227 36.747 64.918

Kontrol 34.085 6.227 20.000 48.170

Post Hoc Tests


Dependent

Variable:Neutrofil

(I)

Perlakuan (J) Perlakuan

Mean

Differe

nce (I-

J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

LSD Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam

ascorbat

-

2.582

5

8.805

71 .776 -22.5024 17.3374

Kontrol 14.16

50

8.805

71 .142 -5.7549 34.0849

Vitamin E

tokotrienol

+ Asam

ascorbat

Vitamin E

tokotrienol

2.582

5

8.805

71 .776 -17.3374 22.5024

Kontrol 16.74

75

8.805

71 .090 -3.1724 36.6674

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Kontrol Vitamin E

tokotrienol

-

14.16

50

8.805

71 .142 -34.0849 5.7549

Vitamin E

tokotrienol +

Asam

ascorbat

-

16.74

75

8.805

71 .090 -36.6674 3.1724


The error term is Mean

Square(Error) = 155.081.

Homogeneous Subsets

Neutrofil

Perlakuan N

Subset

1

Duncana Kontrol 4 34.0850

Vitamin E tokotrienol 4 48.2500


Asam ascorbat 4 50.8325

Sig. .102

Means for groups in homogeneous subsets are

displayed.


The error term is Mean Square(Error) = 155.081.

a. Uses Harmonic Mean Sample Size = 4.000.

68

EOSINOFIL

Tests of Normality

Perlakuan


Statist

ic df Sig.

Statist

ic df Sig.

Eosino

fil

Vitamin E

tokotrienol .298 4 . .927 4 .574

Vitamin E

tokotrienol +

Asam ascorbat

.185 4 . .973 4 .857

Kontrol .298 4 . .849 4 .222


Correction

RAL menggunakan Uji OneWay Anova


Value Label N


tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4

69


Dependent Variable:Eosinofil

Perlakuan Mean

Std.

Deviation N

Vitamin E tokotrienol 2.9175 .68840 4


Asam ascorbat 2.5000 1.03888 4

Kontrol 3.2525 .50056 4

Total 2.8900 .77155 12


Variancesa


F df1 df2 Sig.

1.759 2 9 .227







Source

Type III Sum


Model 101.362a 3 33.787 56.196 .000

Perlakuan 101.362 3 33.787 56.196 .000

Error 5.411 9 .601

Total 106.773 12


70


Perlakuan





Vitamin E tokotrienol 2.918 .388 2.040 3.795


Asam ascorbat 2.500 .388 1.623 3.377

Kontrol 3.252 .388 2.375 4.130

Post Hoc Tests


Dependent

Variable:Eosinofil

(I) Perlakuan (J) Perlakuan

Mean

Differe

nce (I-

J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

LSD Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam

ascorbat

.4175 .5482

9 .466 -.8228 1.6578

Kontrol -.3350

.5482

9 .556 -1.5753 .9053

Vitamin E

tokotrienol +

Asam

ascorbat

Vitamin E

tokotrienol -.4175

.5482

9 .466 -1.6578 .8228

Kontrol -.7525

.5482

9 .203 -1.9928 .4878

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Kontrol Vitamin E

tokotrienol .3350

.5482

9 .556 -.9053 1.5753

Vitamin E

tokotrienol +

Asam

ascorbat

.7525 .5482

9 .203 -.4878 1.9928



Square(Error) = .601.

Homogeneous Subsets

Eosinofil

Perlakuan N

Subset

1

Duncana Vitamin E tokotrienol +



Kontrol 4 3.2525

Sig. .222


displayed.


The error term is Mean Square(Error) = .601.


72

BASOFIL

Tests of Normality

Perlakuan


Statisti

c df Sig.

Statisti

c df Sig.

Basofi

l

Vitamin E

tokotrienol .282 4 . .862 4 .268

Vitamin E

tokotrienol + Asam

ascorbat

.326 4 . .899 4 .424

Kontrol .297 4 . .851 4 .229




Value Label N


tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4

73


Dependent Variable:Basofil

Perlakuan Mean

Std.

Deviation N

Vitamin E tokotrienol 1.2075 .15756 4


Asam ascorbat 1.2900 .20928 4

Kontrol .9150 .49890 4

Total 1.1375 .33888 12


Variancesa


F df1 df2 Sig.

7.978 2 9 .010





74


Dependent

Variable:Basofil

Source

Type III Sum


Model 15.838a 3 5.279 49.878 .000

Perlakuan 15.838 3 5.279 49.878 .000

Error .953 9 .106

Total 16.790 12



Perlakuan





Vitamin E tokotrienol 1.208 .163 .840 1.575


Asam ascorbat 1.290 .163 .922 1.658

Kontrol .915 .163 .547 1.283

75

Post Hoc Tests


Basofil

Games-Howell


Mean

Differe

nce (I-

J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam ascorbat

-.0825 .13098 .81

0 -.4934 .3284

Kontrol .2925 .26159

.55

9 -.6907 1.2757

Vitamin E

tokotrienol +

Asam ascorbat

Vitamin E

tokotrienol .0825 .13098

.81

0 -.3284 .4934

Kontrol .3750 .27051

.42

8 -.5864 1.3364

Kontrol Vitamin E

tokotrienol -.2925 .26159

.55

9 -1.2757 .6907

Vitamin E

tokotrienol +

Asam ascorbat

-.3750 .27051 .42

8 -1.3364 .5864



Square(Error) = .106.

76

LIMFOSIT

Tests of Normality

Perlakuan


Statist

ic df Sig.

Statist

ic df Sig.

Limfo

sit

Vitamin E

tokotrienol .351 4 . .814 4 .129

Vitamin E

tokotrienol +

Asam ascorbat

.378 4 . .765 4 .053

Kontrol .384 4 . .775 4 .064


Correction



Value Label N


tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4

77


Dependent Variable:Limfosit

Perlakuan Mean

Std.

Deviation N



Asam ascorbat 36.3750 13.45010 4

Kontrol 49.8325 4.39069 4

Total 41.6525 11.45613 12


Variancesa


F df1 df2 Sig.

1.650 2 9 .245







Source

Type III Sum


Model 21231.925a 3 7077.308 61.786 .000

Perlakuan 21231.925 3 7077.308 61.786 .000

Error 1030.915 9 114.546

Total 22262.840 12


78


Perlakuan





Vitamin E tokotrienol 38.750 5.351 26.644 50.856


Asam ascorbat 36.375 5.351 24.269 48.481

Kontrol 49.832 5.351 37.727 61.938

Post Hoc Tests


Dependent

Variable:Limfosit


Mean

Differe

nce (I-

J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

LSD Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam

ascorbat

2.3750 7.5679

0 .761 -14.7448 19.4948

Kontrol -

11.082

5

7.5679

0 .177 -28.2023 6.0373

Vitamin E

tokotrienol +

Asam

ascorbat

Vitamin E

tokotrienol

-

2.3750

7.5679

0 .761 -19.4948 14.7448

Kontrol -

13.457

5

7.5679

0 .109 -30.5773 3.6623

79

Kontrol Vitamin E

tokotrienol

11.082

5

7.5679

0 .177 -6.0373 28.2023

Vitamin E

tokotrienol +

Asam

ascorbat

13.457

5

7.5679

0 .109 -3.6623 30.5773




Homogeneous Subsets

Limfosit

Perlakuan N

Subset

1

Duncana Vitamin E tokotrienol +



Kontrol 4 49.8325

Sig. .123


displayed.




80

MONOSIT

Tests of Normality

Perlakuan


Statisti

c df Sig.

Statisti

c df Sig.

Monosi

t

Vitamin E

tokotrienol .173 4 . .981 4 .907

Vitamin E

tokotrienol + Asam

ascorbat

.253 4 . .908 4 .471

Kontrol .304 4 . .790 4 .085




Value Label N


tokotrienol 4

2 Vitamin E

tokotrienol

+ Asam

ascorbat

4

3 Kontrol 4

81


Dependent Variable:Monosit

Perlakuan Mean

Std.

Deviation N



Asam ascorbat 9.2525 1.83409 4

Kontrol 11.5850 .62952 4

Total 9.9183 1.94962 12


Variancesa


F df1 df2 Sig.

2.204 2 9 .166







Source

Type III Sum


Model 1197.371a 3 399.124 144.146 .000

Perlakuan 1197.371 3 399.124 144.146 .000

Error 24.920 9 2.769

Total 1222.291 12


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Perlakuan





Vitamin E tokotrienol 8.918 .832 7.035 10.800


Asam ascorbat 9.253 .832 7.370 11.135

Kontrol 11.585 .832 9.703 13.467

Post Hoc Tests


Dependent

Variable:Monosit


Mean

Differe

nce (I-

J)

Std.

Error Sig.

95% Confidence

Interval

Lower

Bound

Upper

Bound

LSD Vitamin E

tokotrienol

Vitamin E

tokotrienol +

Asam

ascorbat

-.3350 1.1766

3

.78

2 -2.9967 2.3267

Kontrol -

2.6675

*

1.1766

3

.05

0 -5.3292 -.0058

Vitamin E

tokotrienol +

Asam

ascorbat

Vitamin E

tokotrienol .3350

1.1766

3

.78

2 -2.3267 2.9967

Kontrol -

2.3325

1.1766

3

.07

9 -4.9942 .3292

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Kontrol Vitamin E

tokotrienol

2.6675

*

1.1766

3

.05

0 .0058 5.3292

Vitamin E

tokotrienol +

Asam

ascorbat

2.3325 1.1766

3

.07

9 -.3292 4.9942




*. The mean difference is

significant at the .05 level.

Homogeneous Subsets

Monosit

Perlakuan N

Subset

1

Duncana Vitamin E tokotrienol 4 8.9175



Kontrol 4 11.5850

Sig. .058


displayed.




Gambar lampiran 1. Jenis leukosit. a. Monosit, b. Basofil ...

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