Post on 28-Feb-2023
SWITAPASPresentations
ThirdAnnual
SmallWorldInitiativeSymposium
at
AmericanSocietyforMicrobiology
(ASMMicrobe2016)
FromrighttoleftErikaKurt,SWIPresidentandCEOJoHandelsman,SWIFounder
NicholeBroderick,SWIPartnerInstructorLead
SymposiumOrganizingCommitteeJeanSchmidt
UniversityofPittsburghMustafaMorsy
UniversityofWestAlabamaBetsyRoberts
SouthernConnecticutStateUniversityEricWarrick
StateCollegeofFlorida
SWILeadership
AbouttheSmallWorldInitiative
FormulatedatYaleUniversityin2012bythecurrentAssociate
DirectorofScienceattheWhiteHouse,JoHandelsman,the
SmallWorldInitiative™(SWI)isaninnovativeprogramthat
encouragesstudentstopursuecareersinsciencewhile
addressingareal‐worldhealththreat– thediminishingsupply
ofeffectiveantibiotics.SWIcentersaroundanintroductory
biologycourseinwhichstudentsperformhands‐onfieldand
laboratoryresearchonsoilsamplesinthehuntfornew
antibiotics.Throughaseriesofstudent‐drivenexperiments,
studentscollectsoilsamples,isolatediversebacteria,testtheir
bacteriaagainstclinically‐relevantmicroorganisms,and
characterizethoseshowinginhibitoryactivity. Thisis
particularlyrelevantasovertwothirdsofantibioticscome
fromsoilbacteriaorfungi.
SWI’snovelapproachharnessesthepowerofactivelearningto
achievebotheducationalandscientificgoalsandprovidesa
uniqueandsustainableplatformtoreplenishtheantibiotic
pipelinebyidentifyingsuitablecandidatesfortesting.
Currently,SWI’scourseisin108schools(98colleges)across
33USstates,PuertoRico,andtenadditionalcountries– Belize,
Canada,Iraq,Ireland,Jordan,Malaysia,Nigeria,thePhilippines,
andtheUK,andhasimpactedmorethan8,000students.
Levelingdown‐SWItheSummerCampExperience
MichaelBuckholt,Ph.D.
WorcesterPolytechnicInstitute
WhatWeDo:
• 2weeks(actually8days)
• ~20students(campers)
• Welcomeletterwithwarningandwaiver
• 1assistant(usuallyaformerSWIstudent)
• Lotsofliquidnitrogen
WhatICanSay:
• Cangetresultsin8days
• Goodwithbigconceptswithoutallofthecontentdetails
• Recruitsstudentsfortheuniversityandthedepartment
SWIBiosafetyUpdates
KristenButela,Ph.D.
SetonHillUniversity
SWIBiosafetyUpdates
• UpdatedStudentGuide,InstructorGuide,andResearchProtocols
• BiosafetyTrainingPowerpoint withtrainingactivities
• HowtoupgradeBSL‐1labtoBSL‐2standards
• Informationonregulationsforhandlingpotentialhumanandplantpathogens,soiltransport,mailingstrains,etc.
• Recommendationsforimmunocompromised students
SmallWorldInitiativealluringLatin@s intoResearch
Lilliam Casillas,Ph.D.
UniversidaddePuertoRico‐Humacao
SmallWorldInitiativeIntegratedLabs:(1)MolecularandCellBiologyLab– UntilbacterialID’sofisolatesproducingantimicrobials.
(2)IntroductiontoBiotechnology– ChemicalCharacterizationof
antimicrobials.LaboratoryManualsweretranslatedtoSpanish
Accesstoresearchtoallstudentsintheclass=Equity
CyberneticgirlscanbepinkyEquityintheclassroomCulturallysensitiveGenderdifferencesinComputationalBiologyEmpoweringAfrodecedentsWhiteFragilityhttps://www.aacu.org/tides
cienciapr.org
Semestercourse
4daysworkshop
TheMicrobialWorld:thebenefitsofanSWIcourseonnon‐sciencemajorstudents
DebraDavis,Ph.D.WingateUniversity
MoreThanJustD.I.R.T.T:PilotingtheSWIHuntforAntibioticsinHighSchool
BarbaraFishel,Ph.D.
TheHockaday School
Hockaday’sgoalsmatchedtheSWIapproach• Introduceprinciplesofgeneralbiologythroughauthenticresearchandinquiry‐basedlearning.
• Developscientificthinkingskills.• Increaseconfidenceandinterestinpursuingscience.
Studentoutcomesexceededexpectations• InvestedingoalsofSWIandourcourse.• Repetitiongeneratedownershipoflearning,reflectedinengagementandperformance.
• Allninestudentsreportedtheyappliedthebiologytheylearnedwhendesigningexperimentsandinterpretingresults.
Affordablewhole‐genomesequencingof2novelmarineantibioticproducers
BrittanyJ.Gasper,Ph.D.
FloridaSouthernCollege
ExtensionofSWIResearch
• Youfoundsomethingcool!Nowwhat?
• WholegenomesequencingandANI
• Lessexpensivethanyouthink!
• Multilocus sequenceanalysis(MLSA)
• Genusspecific
• Polyphasic Analysis• FAME,biochemical,etc.
AntimicrobialAgents:What’sintheLabel
MaryMiller,Ed.D.
BatonRougeCommunityCollege
AClassroomActivity
Objective:Toexposestudentstocontenttheywillseeintheirlives,andapplywhattheyhavelearnedtounderstandmechanismsofactionsandsusceptibilitytests.
• Pairandshare
• 15‐20minutes
• Pre‐requisites• Prokaryotecellstructureandfunction
• DNAreplicationandProteinSynthesis
Studentsfindthefollowing
informationandsharewiththeclass:
• Description
• Microbiology
• Susceptibility• Kirby‐Bauer• MIC
• AdverseReactions
HighThroughputAntibioticsScreening
MustafaMorsy,Ph.D.TheUniversityofWestAlabama
150mmPetridishes96‐wellplate(0.5ml) 96‐wellPinReplicator
100mmPetridishes100mmPetridishes
TheIsolationandCharacterizationofMarineAntibioticProducingBacteria
EricWarrick,Ph.D.StateCollegeofFlorida
TesterStrain GramReaction/CellularMorphology
Bacillussubtilis GramPositive – RodStaphylococcusepidermidis
GramPositive– Cocci
Chromohalobactersalexigens
GramNegative– Rod
Vibriofischeri GramNegative– RodVibrioanguillarum GramNegative– Rod
Whatdoesyourbackyardlooklike?AtStateCollegeofFlorida– Bradenton,FloridaI’veadaptedtheSWIcurriculumtofocusonmarineantibioticproducingbacteria.
Whatdoyouneedtoculturemarinebacteria?
‐ Supplementthemedia0.5MNaCl(PDA,LB,TSA,andR2A)
‐ MarineAgar
Howwillthetesterstrainsgrowonthemediasupplementedwith0.5MNaCl?
What HaveWeFound?Vibriospecies
Pseudoalteromonas species
Photobacterium species
Halobacillus species
Streptomycesspecies
Halomonas species
Zooshikella species
PinkPigmentedVibrioisolate
RedPigmentedZooshikellaIsolate
PhotobacteriumIsolate
IdentificationofaBiosurfactant bywayoftheSWI
ElizabethLewisRoberts,Ph.D.SouthernConnecticutStateUniversity
AtomizedOilDropletAssay
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1
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3
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BigR BlackKrim
DroughtSeverity
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