01/11/2010 __________________________ Signature

Luis C. Afonso, M.D., FACC Associate Professor of Internal Medicine

Division of Cardiology Wayne State University, Detroit, MI

Office Address: Harper University Hospital 8 Brush, Division of Cardiology 3990 John R Detroit, MI 48201 Office:745 2620 Fax: 745 8643 lafonso@med.wayne.edu EDUCATION 1980-1984 MBBS Degree, Goa Medical College, Panjim, Goa affiliated to the University of

Bombay, India POSTGRADUATE TRAINING 1984-1986 Rotating Internship, Goa Medical College Affiliated Hospitals, Goa India 1986-1989 Junior Residency Internal Medicine, Goa Medical College Affiliated Hospitals,

Goa, India 1989-1993 Senior Residency Internal Medicine, Goa Medical College Affiliated Hospitals,

Goa, India 1993-1996 Residency Internal Medicine, Wayne State University Affiliated Hospitals,

Detroit, MI 1996-1997 Fellowship in Geriatrics, Wayne State University Affiliated Hospitals, Detroit, MI 1997-2000 Fellowship in Cardiology, Wayne State University Affiliated Hospitals, Detroit,

MI

2

FACULTY APPOINTMENTS 2001-2009 Assistant Professor, Internal Medicine Cardiology, Wayne State University,

Detroit, MI 2002-present Program Director, Cardiology Fellowship Training Program, Wayne State

University, Detroit, MI 2009-present Associate Professor, Internal Medicine Cardiology, Wayne State University,

Detroit, MI HOSPITAL OR OTHER PROFESSIONAL APPOINTMENTS 2001-2004 Director, Echocardiography Laboratory, John D Dingell VA Medical Center,

Detroit, MI. 2002-present Consultant Cardiologist, Renal and Pancreatic Transplant Service, Detroit

Medical Center, Detroit, MI 2004-present Director, Echocardiography Laboratory, Harper University Hospital,

Detroit, MI MAJOR PROFESSIONAL SOCIETIES Member of the American Heart Association Fellow, American College of Cardiology, FACC Member, American Society of Echocardiography Member Steering Committee Michigan Society of Echocardiography Associate, American College of Physicians Member, American Medical Association MEDICAL LICENSURE DEA License 2002 (Active) State of Michigan: Permanent Physician License #4301062290 (Active) Controlled Substance License #4301062290 (Active)

3

BOARD CERTIFICATION 1984 MBBS Degree, University of Bombay, India 1989 Postgraduate MD Degree, Internal Medicine, University of Bombay, India 1992 FMGEMS II (Clinical Sciences) 1993 Federal Licensing Examination (FLEX Components I & II) 1993 Foreign Medical Graduate Exams in the Medical Science FMGEMS (Basic Science) 1996 Certified American Board of Internal Medicine 1996-2006 1998 Certified American Board of Internal Medicine, Special Qualification Geriatric Medicine 1998-2008 2000 Certified American Board of Internal Medicine, Cardiovascular Disease 2000-10 2002 Radiation Physics Course (200 hrs) required by Nuclear Regulatory Commission for NRC licensure 2003 Testamur, Board Certification, American Society of Echocardiography 2007 Level II Cardiac Computerized Tomographic Angiography (CCTA) HONORS/AWARDS 1984 Ranked First in MBBS Class of 1984 and Second in Internal Medicine, University of

Bombay 1989 Ranked First in Class of 1989, M.D. Degree Exam. Goa Medical College University of

Bombay 1997-98”Outstanding Fellow of the Year” award, Division of Cardiology, Wayne State

University, Detroit Medical Center, Detroit, MI 1998 Award Winner, American College of Cardiology, Michigan Chapter, Clinical Vignette,

Competition, Fall 1998 1998-99 “Best Teaching Fellow Award” selected by Residents in the Internal Medicine

Residency Program, Wayne State University, Affiliated Hospitals, Detroit, MI 199-00 Chief Fellow, Division of Cardiology, Wayne State University, Detroit, MI

4

2000 “Abstract selected for the “Young Investigator Award” Competition of the American

Society of Nuclear Medicine Annual Meeting June 2000 2005 “Academic Teacher of the Year” award 2004-05. Division of Cardiology, Wayne State

University, Detroit, MI 2006 “School of Medicine Teaching Award” Wayne State University, School of Medicine,

Detroit, MI

SERVICE 2002-09Assistant Professor of Medicine Department of Internal Medicine Cardiology, Wayne

State University School of Medicine. UPG 4 C Clinic, Geriatrics Clinic 5B Consulting 2002-present Consultant Cardiologist, Renal and Pancreatic Transplant Service,

Detroit Medical Center, Detroit, MI

2009-present Associate Professor of Medicine Department of Internal Medicine Cardiology, Wayne State University School of Medicine. UPG 4 C, Geriatrics Clinic 5 B

Roles:

1. Patient Care: Clinical Rounder in the Coronary Care Unit and Consult Service 3 months of the

year. Super clinical rounder 12 weekends a year University Health Center Cardiology Outpatient Clinic: Supervisor of 6 trainees

half day/week Geriatric Cardiology Clinic Consultant 5 B UHC: Supervising 2-3 Fellows ½ day

alt weeks.

2. Echocardiography Annually responsible for interpretation of roughly:

6500 surface echoes 650-700 Transesophageal echoes 600 Exercise or Doubatine echoes 144 specialized imaging studies such as dsynchrony analysis, strain imaging,

tissue Doppler and 3-D imaging

3. Electrocardiography Reading for the DMC Average of four-2 hr slots a month, approximately 12000 ECG’s annually

4. Digital Transition of Harper Echo Lab

Responsible for conceiving and implementing analog (video tape) to Digital transition of the Echo Lab, upgrading imaging equipment and implementing electronic reporting. The University Lab offers “state of the art” services such as 3-D imaging. Tissue Doppler,

5

Tissue synchronization, strain and speckle tracking imaging. Electronic reporting was also phased in allowing for efficient, timely and cost-effective transmission of echo reports to the hospital database.

5. Medical Director, Harper University Hospital Quality Analysis and Improvement Meetings and Measures Maintaining and implementing Echo Procedures/Protocols of the DMC Compilation of requirements for Lab accreditations. Undertake and conduct monthly CME for songraphers and nursing staff

Recent effort leading on to accreditation of Harper and DRH Echo labs by the prestigious Intersocietal Commission for Accreditation of Echocardiography Laboratories (ICAEL), certificate valid to 2011.

6. Cardiology Fellowship Training, Program Director

Overwhelming contribution to Core Curriculum document compilation Development of Division Trainee In-Service exam Regular formal and bedside didactics Faculty Mentoring Clinical research mentoring, weekly session.

7. Applicant Interviews:

This very time consuming responsibility and comprises a labor-intensive adjudication and interviewing of >100 candidates for 4 Fellowship positions. Short listing done after careful review of 540 applicant folders including letters of recommendations, USME scores and research accomplishments.

8. DMC Renal-Pancreatic Transplant Team Cardiology Consultant

Responsible for risk stratification and preop assessment of transplant candidates, weekly clinical appraisal sessions.

9. Internal Site Reviewer: Participated as one of 2 expert reviewers in the mid cycle

Internal review of the Cardiothoracic Residency Training Program 10. Annual Meeting Abstract Reviewer. Michigan Chapter, American College of

Physicians, Scientific Meetings.

11. Member Pharmacy and Therapeutics Committee, Harper University Hospital 2007 to present.

Community:

Women and Heart Disease, Feb 2009. Lay person lecture to parishioners, St. Thomas Aquinas Parish, Dearborn Heights, MI

6

DMC Annual Health Fair, Fox Theatre, Detroit, MI (2007-present) . Annual Screening echoes 1 full day for employees of Fox Theatre. Entails limited screening of roughly 50 Employees. DMC Annual Health Fair Compuware Employees (2007-present). Annual Screening echoes, 1 full day for employees of Compuware. Entails limited screening of roughly 45-50 Employees.

Consulting:

2002-present Consultant Cardiologist, Renal and Pancreatic Transplant Service, Detroit Medical Center

** Other: Annual NBA Mandate Preseason Screening and Physicals for the Detroit Pistons (2006 – present)

Responsible for coordinating Physicals and Stress echo testing as part of the annual preseason screening mandated by the NGA for professional basketball players. This is a major undertaking, held annually at the Palace of Auburn Hills, entails transportation of echo machines, treadmills, etc to the Palace, an all day affair starting with physicals, culminating with exercise stress testing for all players, subs and coaches. This is a high profile, prestigious, gratuitous service that the DMC provides and is coordinated by me that incidentally received wide press coverage this year (Detroit Free Press).

Scholarly Service Service for Peer-Reviewed Journals Manuscript Reviewer 2005-present: Chest (2 Manuscripts) 2006-present: Clinical Cardiology (15 Manuscripts) 2007: American Journal Cardiology (8 Manuscripts) 2007: Circulation (1 Manuscript) Regional Service Contributions 2008-09 Michigan Society of Echocardiography, Member Steering Committee. This is a prestigious position and regional recognition of expertise in the field of Echocardiography. Role entails attending (Quarterly Meetings) actively participate in discussion, planning etc.

7

TEACHING Years at Wayne State University 2001-present, 8 years at Wayne State (of these 3 years spent at the VA as full time faculty) Course taught at Wayne State in the last 5 years: 2nd Year Wayne State University Medical Students:

Annual Pathophysiology Lectures series Annual Physical Diagnosis Instruction Annual Clinical Cardiovascular Exam lecture

3rd Year Wayne State University Medical Students on Clinical Rotation 3rd Year Wayne State University Medical Students Physical Diagnosis Instruction 4th Year Wayne State University Medical Students on Elective Rotation (one-on-one teaching) WSU First Year Cardiology Fellow Clinical Consult Rotation (one-on-one) Teaching: WSU First Year Cardiology Fellow Cardiac Intensive Care Rotation (one-on-one) Teaching WSU Year I, II, and III Cardiology Fellow Echocardiography (one-on-one) Teaching WSU Cardiology Fellow Mentor (6Fellows) WSU Cardiology Fellow Didactic Teaching: Core Curriculum Lecture throughout the year with a focus on:

Echocardiography Mentoring of Clinical Research and Scholarly activities Faculty Mentoring

Bedside Teaching: Consistently impart clinical skills at the bedside to trainees ranging from medical students to rotating residents and fellows ranging from bedside clinical exam skill to ECG’s nuclear perfusion and diagnostic angiography interpretation. Ambulatory Clinic Supervision: Responsible for close supervision of fellows and rotating residents with a considerable attention to bedside skills, treatment guideline adherence, cost effective care instruction, preventive cardiology, risk factor modification and care plan assessment at 2 Clinic sites: 1. University Health Center Cardiology Clinic 4C and 2. Geriatric Cardiology 5 B University Health Center, Geriatric Center of Excellence

8

Echo Lab Interaction: Shoulder to shoulder performance and interpretive instruction to Cardiology Fellowship trainees throughout the year. Extensive time commitment to this activity, involves technology updates, digital processing, special techniques and instrument handling etc. Resident Teaching: Regular formal Cardiology Series lectures and Board Review 4-6 sessions during the academic year. Particular focus on Arrhythmia Management, ECG interpretation and clinical exam skills. Training of interested residents in echo interpretation during monthly noninvasive rotation. Research Mentor for residents conducting cardiology related research. Interdisciplinary Lectures: Cardiothoracic Resident teaching, cross-disciplinary lectures and instruction during joint weekly conferences. Physical Diagnostic Course Participant: Regular contributor to this annual effort since 1999. (6 sessions x medical students, annually) Physician Shadowing: Hosted countless premed and accelerated track WSU (MEDSTART) medical students as part of shadowing experience to expose trainees to the daily routine of an attending physician over the course of the year. MENTORING: (includes clinical research projects and scholarly activity) ** The overwhelming majority of abstracts and articles have been collaborative efforts with trainees at all levels from residency to fellows. A sampling is listed below. Due to the large volume, have opted not to list all mentees, please refer to the abstract / publication section where trainees are identified by asterisks* 2006-present. (All projects listed below culminated in Published Article/Abstracts) Veeranna V PGYIII and Fakhry H PGYIII Severity of Coronary Artery Disease in Obese Patients Undergoing Coronary Angiography and Intervention (Data Published Clin Cardiol. 2007) Correlation of Lipid Profile Undergoing coronary angiography. American Heart Association Quality of Care and Outcomes Research in Cardiovascular Disease and Stroke Conference, April 2009, Washington DC. Niraj Ashutosh PGYII Severity of coronary artery disease in obese patients undergoing coronary angiography “obesity paradox” revisited. Published article Clin Cardiol. 2007 Ethnic and Gender Differences in Disease Burden in Patients Undergoing Coronary Angiography: The Confounding Influence of Obesity. Accepted for publication Ethnicity and Disease.

9

Tamam Mohammad PGYII Cocaine-Related Chest Pain _ Utility of TIMI Risk Stratification and Effects of Beta Blockers on Outcome. Published Supplement Journal American Coll Card. JACC 47(4); 288A Feb 2006 Crack Whips the Heart: A comprehensive Review of the Cardiovascular Toxicity of Cocaine. Am J Cardiol. 2007 Sept 15;100(6):1040-3 Role of Cardiac MRI in Peripartum Cardiomyopathy: Ongoing study of newly diagnosed patients with PPC exploring the etiologic role of inflammatory myocarditis and hyperprolactenemia Full length article Cocaine-Induced Chest Pain and Beta-Blockade: An Inner City Experience Am J Ther. 2008 Nov-Dec;15(6):531-5 Ivan Hanson PGYI and Rayan Hourani PGYII Comparison of Two-Dimensional versus Three-Dimensional Echocardiography Techniques to Measure Left Atrial Volume: Correlation with Left Ventricular Filling Pressure (Abstract published 18th Annual Scientific Session of the American Society of Echocardiography (ASE) Do Mitral Annular and Inflow Velocities Correlate with Indices of Left Atrial Mechanical Function Derived Volumetrically by 3-Dimensional Echo? Presented at the 18th Annual Scientific Sessions of the American Society of Echocardiography (ASE) Danialdo Rodrigues PGYII Submassive Pulmonary Embolism: To Lyse or not to Lyse? Presented 30th Annual Society for General Internal Medicine Scientific Session, April 2007 Terefe Yared PGYII Myocardial Infarction with Angiographically Normal Coronary Arteries Abstract. Full length article published in J Coron Artery Dis 2007 Khaled Awad PGYIII Midventricular Ballooning Cardiomyopathy: Epicardial spasm vs. Microvascular Dysfunction: Can Echo Identify the Culprit? Second Annual Cases Competition at the 18th Annual Scientific Sessions of the American Society of Echocardiography (ASE) Seattle, WA June 16-20, 2007

10

Bandaru Himabindu PGYII Predictors of Cardiac Troponin – I Release in Patients with Hypertensive Emergency. XIVth Scientific Sessions of the Inter-American Society for Hypertension (IASH) and the Consortium for Southeastern Hypertension Control May 2007 Mengistu Simegn, PGYII Loss of Base to Apex Strain Gradient and Low Global Longitudinal Strain Identifies Hypertrophic Cardiomyopathy From Outer Forms of Left Ventricular Hypertrophy. Abstract accepted American Society of Echocardiography Annual Scientific Session, Orlando, FL 2009 Strain Dispersion Index and Global Longitudinal Strain in Differentiating Hypertrophic Cardiomyopathy From Other Forms of Left Ventricular Hypertrophy. Abstract Accepted for the American College of Cardiology 58th Annual Scientific Session, Orlando, FL 2009 Anil Mathew PGYII Tei Index and Spectrum of Diastolic Abnormalities in Takotsubo Cardiomyopathy 14th World Congress on Heart Disease. The International Congress on Heart Disease Annual Scientific Sessions, Toronto, Canada July 2008 Anupama Kottam, PGYII Improving Diagnostic Yield of Transesophageal Echocardiography for Patent Foramen Ovale: Can Fluid Bolusing Release the Door-Jam? Oral Abstract to be presented American Society of Echocardiography Annual Scientific Session Orlando, Fl 2009 Jyotiranjan Pradhan PGYI Determinants of Amount of Contrast Utilized in Patients Undergoing Percutaneous Coronary Procedures. Coron artery Dis. 2007 Anil Mathew PGYII Does the Tei Index Characterize Cardiac Performance in Takotsubl Cardiomyopathy? Congress on Heart Disease. The International Congress on Heart Disease Annual Scientific Session, Toronto Canada July 2008 Shalini Modi Fellow YRIII

11

Unusual Pulmonary Venous Flow Profile in a Patient with mitral Valve Perforation secondary to Bacterial Endocarditis. Published article Echocardiography. 2006 Feb;23(2):137-9 Gender, Carotid Stenosis And Risk Factor Treatment in African American Patients with CAD. Second International Conference in Women, Heart Disease and Stroke, American Heart Association 2005 Juan Bernal Fellow YRII The Echocardiographic assessment of Hypertrophic Cardiomyopathy: Emphasis on Novel Imaging Modalities. (Under review for J Amer College of Cardiology Imaging 2008) Acute Pulmonary Edema Following Pericardiocentesis for Cardiac Tamponade. Published Can J Cardiol. 2007 M. Reddy Fellows YRII Apical Hypertrophic Cardiomyopathy: Potential Utility of Strain imaging in Diagnosis. Published Article EUR J Echo 2007 Sridevi Pitta Fellow YRII Metastatic Extracavitary Cardiac melanoma: The Role of Contemporary Imaging Techniques in Diagnosis and Surgical planning. Published Circulation 2007 Incremental Utility of Real-time Three Dimensional Echocardiography in the Diagnosis and Pre-operative Assessment of Cleft mitral Valve in Adults. Published European Journal Echocardiography Thromboembloic Complications of Left Ventricular Noncompaction-Case Report and Brief Review of the Literature J Clin Ultrasound. 2007 May 7;35(8):465-468 Quadricuspid Aortic Valve associated with Unruptured Sinus of Valsalva Aneurysm Accepted for publication European Journal Echocardiography Ref: Ms. No. EJE. 07.387.1574R1 Hui Jin YRIII Recurrent Heart Failure with preserved Ejection fraction but Markedly elevated BNP in a 51 year-old Female on Hemodialysis with oversized AV Fistula. Published Int J Cardiol 2006 Khaled Bachour Fellow YRII Takotsubo Cardiomyopathy: Pathogenetic Insights and Myocardial Perfusion Kinetics Using Myocardial Contrast Echocardiography. Accepted for publication European J of Echocardiography 2008 Ms. No. EJE. 08.103.1692RI

12

Significance of Valvular Perforation in Left-Sided Infective Endocarditis. (Novel dataon the significance of Valvular Perforation in Endocarditis) FACULTY MENTORING: both have several abstracts in national meetings and peer-reviewed publications

Ashok Knodur Mahahan Nitin

Sampling of Ongoing Activity with Interns/Residents and manuscripts near completion Kottam A, Afonso L. Improving Diagnostic Yield of Transeophageal Echocardiography for Patent Foramen Ovale: Can Fluid Bolusing Release the Door-Jam? Vikas Veeranna, Ashutsoh Niraj, Hesham Fakhry, Jyotiranjan Pradhan, Luis Afonso. Correlation of Lipid Profile and Angiographic Coronary Artery Disease Burden Among Elderly patients Undergoing Coronary Angiography. Apurva Badheka, Ankit Rathod, Mohamad A. Kizilbash, Xongshan Lai, Neh Garg, Samrat Bhat, Luis Afonso, Sony Jacob. Implantable Cardiac Defibrillator Shocks Do Not Predict Death in a Secondary Prevention. Bhadheka, Neha Garg, Mohammad A. Kizilbash, Samrat Bhat, Ankit Rathod, Sony Jacob, Luis Afonso. Incident Diabetes Mellitus in Patients with Stable Coronary Artery Disease Mengistu Alemayeh Simegn, Ashok Kondur, Preeti Ramappa, Tamam Mohammad, Luis Afonso. Strain Dispersion Index and Global longitudinal Strain in Differentiating Hypertrophic Cardiomyopathy From Other Forms of Left Ventricular Hypertrophy. Mengistu Alemayehu Simegn, Firas Zahwe, Khaled Bachour, Tamam Mohamad, Kidmealem Zekarias, Preeti Ramappa, Luis Afonso, Deepak Thatai. Cardiac Troponin-I, In-Hospital Mortality and the Need for Emergency Valve Replacement Surgery in Infective Endocarditis. COURSE/ CURRICULUM DEVELOPMENT Cardiology Core Curriculum Document Spearheaded effort to develop an extensive and comprehensive Core Curriculum for the Cardiology Fellowship. This extensive document was compiled and serves as the official guideline document and course syllabus for the Fellowship training program. Fellowship Policy Manual Compiled a complete set of documents that outline the Fellow operations, responsibilities and code of conduct in accordance with ACGME guidelines. This document details rules and

13

regulations relating to duty hours, order writing, moonlighting, relationship to private faculty, and due process. Booklet for the Applicant Compiled a booklet that is handed out to the applicant that comes for an interview with the department. It outlines the responsibilities of the fellow once they enter into the program. Outlines the clinical rotation, echo, nuclear, EP, and cath rotation. Monthly Conference Schedule Relates to assembling and planning relevant conference topics, lectures and speakers for monthly didactics including Cardiology Grand Rounds In-service Training Exam 2006-present Instrumental in developing an annual Medical Knowledge assessment instrument to help longitudinally evaluate/monitor Fellow training and process Monthly Structured Testing 2007-present Responsible for and executing and developing this knowledge assessment metric used to monitor trainee process in the field of Cardiology based on preassigned syllabus based on a core curriculum. (Entails setting ~50 multiple choice questions on a peaessigned topic). Performance tracking and remediation of areas of weakness now possible with this tool. Spectacular results with 100% passage rates in General Cardiology. Echocardiography and Nuclear Cardiology Boards, activity highly regarded by trainees. MCQ Tests designed and completed include:

Valvular HD and Endocarditis Congestive Heart Failure Electrophysiology Pericardial Diseases Pulmonary Hypertension

Each of these tests entails a set of 50 MCQ’s on the assigned topic from Braunwalds Textbook of Cardiology, are exclusively for our program and is a very time consuming exercise. Over 90% of the questions sets in aggregate were developed by me. We use the results to monitor Fellow training and these become part of the performance record in their files. Cardiology Fellowship Accreditation: In 2008 the WSU/DMC Adult Cardiovascular Fellowship trading Program underwent a site visit for continuing accreditation. As Program Director, I was directly responsible for safeguarding our accreditation status. Preparation for this visit was exhaustive and thorough. We were awarded continuing ACGME accreditation for an additional 5 years without a single citation: a remarkable achievement for the program, unprecedented in my tenure as Program Director. The Program can boast of 100% passage rate in the Annual ABIM Cardiology Board Exams, National Board of Echocardiography Exams, the American Nuclear Cardiology Society and the Society for Cardiac CT (SCCT)Exams. Two of our graduating Fellows landed prestigious Interventional Fellowship positions at the Massachusetts General Hospital and

14

Mayo Clinic Interventional Programs, testifying to the caliber of trainees graduating from our Program. GRANTS, CONTRACTS, AND OTHER FUNDING 2009 In review NIH-NIA T35 eight week Summer Research Program Enhancing Geriatric Assessment Research skills and knowledge (EGAR). Co-PIs Jennifer Mendez, PhD, WSU SOM and Luis Afonso, MD, FACC Role of Cardiac magnetic Resonance Imaging in the Diagnosis of Peripartum Cardiomyopathy submitted for WSU intramural grant support: not funded 2008 CLINICAL TRIALS ACTIVITIES 1999 Co-investigator and Collaborating investigator in successful grant proposal for intramural funding for research titled “Effects of Adenosine on Microvascular Function in Patients with Acute myocardial Infarction” JR Spears, Luis Afonso, Eric Racine, James Janisse, Marcelo Di Carli. 2001 Co-investigator STICH Trial The Surgical Treatment for Ischemic Heart Failure assessing the benefits of aneurysmectomy in improving LV geimetry and systolic function. Active National/International Grants and Contracts 2002 Co-investigator Irbesartan in Heart Failure with preserved Systolic function (I-PRESERVE) Sponsored by Bristol-Myers Squibb. (VA Medical Center) Effects of Angiotensin receptor blocker Irbesartan on left ventricular systolic and diastolic function. 2002-03 Sub PI for A-HEFT (African-American heart failure Trial) VA Medical Center The assessment of Hydrallazine-Nitrates combination on the morbidity and mortality of Congestive Heart Failure in African-Americans 2007 Cardiology PI A multicenter, Randomized, Double-Blind, Placebo-Controlled Study to Evaluate the Safety and Efficacy of SCH530348 in Addition to Standard of Care in Subjects With a History of Atherosclerotic Disease: Thrombin Receptor Antagonist in Secondary Prevention of Atherothrambotic Ischemic Events (TRA 2’P-TIMI-50) 2009 Co-investigator with Dr. Jeffrey Zondee (PI), Karmanos Institute. Study of Multiple Myeloma and Cardiac Amyloidosis: will serve as Core Echo Lab for Multicenter National Trial.

15

PUBLICATIONS: I am the senior or lead author in nearly all below listed publications Original Peer-Reviewed Publications

1. Afonso L, Sowers JR. Effects of Advancing Age on Cardiovascular Baroreflex Function Proceedings of the 3rd International Congress of Hypertension in the Elderly, Feb. 1995;95-107

2. Afonso L, Dihenkar G, Alangaden J. Leptospirosis: Unusual cause of Fever, Jaundice and Renal Failure. Infectious Diseases in Clinical Practice 1997;6(3):203-205

3. Sowers JR, Raman BB, Afonso LC, Bedford-Rice K, Standley PR. Effect of Antihypertensive Therapy on Platelet Cytosolic Calcium Responses to Low Density Lipoprotein Cholesterol. Journal of Human Hypertension 1996;10:177-180

4. Afonso LC, Edelson GW, Sowers JR. Metabolic abnormalities in Hypertension. Current Opinion in Nephrology and Hypertension. 1997;6:219-223

5. Di Carli M. Afonso L, Campisi R, Ramappa P, Bianco-Battles D, Grunberger G, Schelbert H. Coronary vascular dysfunction in premenopausal women with diabetes mellitus. American Heart Journs 2002;144:711-718

6. *Reddy M, Thatai D, *Bernal J, Afonso L. Aptical Hypertrophic Cardiomyopathy: Potential Utility of Train Imaging in Diagnosis. Eur J Echocardiogr. 2007May 27,

7. *Pradhan J, Niraj A, Afonso L. Determinants of Amount of Contrast Utilized in Patients Undergoing Percutaneous Coronary Procedures. Coron Artery Dis. 2007: June;18(4):275-82

8. *Niraja A, *Pradhan J, *Veeranna V, *Fakhury H, Afonso L. Severity of Coronary Artery Disease in Obese Patients Undergoing Coronary Angiography:”Obesity Paradox” revisited. Clin Cardiol. 2007 Aug;30(8):391-6

9. *Teref YG, *Niraj A, *Pradhan J, *Kondur A, Afonso L. Myocardial infarction with Angiographically Normal Coronary Arteries in the Contemporary Era. Coron Artery Dis. 2007 Dec;18(8):621-6

10. Afonso L, *Niraj A, *Pradhan J, Veeranna V, Fakhry H. Ethnic and Gender Differences in Disease Burden in Patients Undergoing Coronary Angiography: The Confounding Influence of Obesity. Ethn Dis. 2008 Winter;18(1):53-8

11. Kondur A, *Pitta SR, Afonso L. Incremental Utility of Real-time Three Dimensional Echocardiography in the Diagnosis and Preoperative Assessment of Cleft Mitral Valve in Adults. Accepted for publication. Eur J Echocardiogr. 2008;9(4):586-8. Epub 2008 Mar 5.

16

12. Kondur A, Tao L, Vaitkevicius P, Afonso L. Quantification of Myocardial Iron

Overload by T2* Cardiovascular Magnetic Resonance Imaging. Accepted Clinical Cardiology Manuscript ID CLC-07-0502. 2008 April, Epub Ahead of Print

13. *Pradhan J, Venkalaya H, *Niraj A, Kumaravelu P, *Trivedi M, Afonso L, Thatai D. QT Dispersion at Rest and During Adenosine Stress Myocardial Perfusion Imaging Correlation with Myocardial Jeopardy Score. Clinical Cardiology, 2008 May;31(5):205-10

14. *Pradhan J, *Schreiber T, *Niraj A, Veeranna V, Ramesh K, Saigh L, Afonso L. Comparison of Five Year Outcome in African Americans versus Caucasians Following Percutaneous Coronary Intervention. Catheter Cardiovasc Interv 2008:Jul1;72(1):36-44

15. Afonso L, *Khaled B, Khaled A. Takotsubo Cardiomyopathy: Pathogenetic insights and Myocardial Perfusion Kinetic Using Myocardial Contrast Echocardiography. Eur J Echocardiogr. 20008 Nov;9(6):849-54

16. *Mohamad T, *Kondur A, Vaitkevicius P, *Bachour K, Thatai D, Afonso L. Cocaine-Induced Chest Pain and Beta-Blockade: An Inner City Experience. AM J Ther. 2008 Nov-Dec;15(6):531-5

17. Mahajan N, Ganguly J, Simegn M, Bhattacharya P, Shankar L, Madhavan R, Chaturvedi S, Ramappa P, Afonso L. Predictors of Stroke in Patients with Severe Systolic Dysfunction in Sinus Rhythm: Role of Echocardiography. Int J Cardiol. 2009 Ju 9 (Epub ahead of Print) PMID: 19520443

18. Afonso L, Pradhan J, Veeranna V, Niraj A, Jacob S. Global and Regional left Ventricular Contractile Impairment in Patients with Wolff-Parkinson-White Syndrome Indian Pacing and Electrophysiology J 2009 Jul. 1;9(4):195-206

19. Khetarpal V, Mahajan N, Madhavan R, Batra S, Mopala P, Sagar A, Rapolu P, Nangia S, Afonso L, Calcification Aortic Valve and Spontaneous Embolic Stroke: A Review of Literature J Neurol Sci. Aug 2009 A 25

20. *Mohamad T, *Niraja A, Obideen, *Farah J, *Badheka A, Kondur A, Thatai D, Afonso L. Spectrum of Electrocardiographic and Angiographic Coronary Artery Disease Findings in Patients with Cocaine-Associated Myocardial infarction. Accepted to Journal of Coronary Artery Disease Coron artery Dis. 2009 Aug;20(5):332-6

21. *Veeranna V, *Pradhan J, *Niraj A, *Fakhry H, Afonso L. traditional Cardiovascular Risk Factors and Severity of Angiographic Coronary Artery Disease in the Elderly Accepted for Publication J of Preventive Cardiology, 2009

17

22. Afonso L, *Kottam A, *Khetarpal V. “Myocardial Cleft, Crypt, Diverticulum or Aneurysm? Does it Really Matter?” Clinical Cardiology, 2009 Aug32(8):E48-51

23*Bachour K, *Zmily H, *Kizilbash M, *Awad K, *Hourani R, Hammad H, Sobel JD, Ghali JK, Levine D, Afonso L. Significance of Valvular Perforation in Left-Sided Infective Edocarditis. MS#CLC-08-0246.RI Clin Cardiol 2009 Dec;32(12):E55-62

24. Mahajan N, Povalaram L, Venkalaya H, Ference B, Want Y, Ager J, Kovach J, Afonso

L. Diagnostic Accuracy of Single Photon Emission Computed Tomography Myocardial Perfusion Imaging and Stress Echocardiography for the Diagnosis of Left Main and Triple Vessel Coronary Artery Disease: A Comparative Meta-Analysis. Accepted ID#:Heart JNL/2009/182295

25. Manickam P, Rathod A, Badheka A, Aravindhakshan R, Afonso L, Jacob S. Effect of Statins on Ventricular Tachyarrhythmias in Patients with implantable Cardioverter Defibrillator-A Meta-Analysis CIRCULATIONAHA/2009/919571 MS

26. *Veeranna V, *Pardhan J, *Niraj A, *Fakhry H, Afonso L. Traditional Cardiovascular Risk Factors and Severity of Angiographic Coronary Artery Disease in the Elderly. Accepted for Publication J of Preventive Cardiology 2009

27. Afonso L, Mahana N. Single Photon Emission Computed Tomography (SPECT)

Myocardial Perfusion Imaging (MPI) in the Diagnosis of Left Main Disease. Accepted for Publication Clin Cardiol. 2009 Dec;32(12):E11-5-PMID:20014205 (PubMed –in process)

Case Reports (Peer reviewed and published/accepted)

1. *Modi S, Koul D, Afonso L. Unusual Pulmonary Venous Flow Profile in a Patient with Mitral Valve Perforation secondary to Bacterial Endocarditis. Echocardiography, 2006 Feb.23 (2): 137-9

2. *Pitta SR, Kondur A, Afonso L. Metastatic Extracavitary Cardiac Melanoma: The Role

of Contemporary Imaging Techniques in Diagnosis and Surgical Planning. Circulation 2007 Apr 14;115 (16) :e415-7

3 *Pitta S, Thatai D, Afonso L. Thromboembolic Complications of Left Ventricular

Noncompaction Case Report and Brief Review of the Literature. J Clin Ultrasound. 2007 May 7;35(8):465-468

4. *Jin H, Afonso L, Singh A, Midgal S, Spears JR. Recurrent heart failure with preserved

ejection fraction but markedly elevated BNP in a 51 year old female on hemodialysis with oversized AV fistula. Int J Cardiol. 2006 June 28:110(3):429-30

18

5. Lakireddy Dr, Li T, Afonso L, Kondur AK. Is Acute Back Pain a Warning Sign for

Penetrating Atherosclerotic Ulcer? (in press) American Journal Medicine, 2007

6. *Penugonda N, Afonso L. Complex Endocarditis in an Immunocompromised Host: The Role of 3-Dimensional Echocardiography. J Am Soc Echocardiogr. 2007 Nov;20(11):134e9-11

7.*Bernal J, Pradhan J, Tchokonte R, Li T, Afonso L. Acute Pulmonary Edema Following

Pericardiocentesis For Cardiac Tamponade. Can J Cardiol. 2007 Dec;23(14);1155-6 8.*Pitta SR, Kondur A, Afonso L. Quadricuspid Aortic Valve associated with Unruptured

Sinus of Valsalva Aneurysm. Accepted for publication European Journal Echocardiography. Ref: MS No EJE.07.387.1574R1

9. Kondur A, Afonso L, Berenbom LD, Lakkireddy Dr. Implantable cardioverter

defibrillators save lives from lightning-related electrocution too! Pacing Clin Electrophysiol. 2008 Feb;31(2):256-7

10. Mahajan N, Afonso L. Right Ventricular Extension of Inferior Wall Myocardial

Infarction: Role of Right Sided Electrocardiogram. South Med J. 2008 Dec; 101(12):1276-7

11. Hari P, Afonso L. An unusual case of Undifferentiated Connective Tissue Disease

presenting as Cardiac Tamponade. Rheumatology International Ref.: Ms.No. RHEID-09-00708 (Clinical and Experimental investigations)

12.*Matthew A, Afonso L, Jacob S. An unusual case of diaphragmatic stimulation after

cardiac resynchronization therapy. Europasce Apri;11(4):411, Epub 2009 Mar 5

13.Jacob S, Kondur A, Khetarpal V, Afonso L. reversal of right ventricular apex pacing-induced left ventricular apical dyskinesis: utility of intraoperative 3-D echocardiography in resynchronization therapy. Circ Arrhythm Electrophysiol. 2009 Aug;2(4):e21-3

14.Mahahan N, Khetarpal V, Afonso L. Stroke secondary to calcific bicuspid aortic valve:

Case report and literature review. Cardiolo. 2009 Aug;54(1):158-61

15.Veeranna V, Pradhan J, Ference B, Afonso L. “Complex Intervention of an In-Stent Restenosis of Ostial Right Coronary Artery stent protruding into Aortic Sinus of Valsalva – A Case Report and Review of Literature J Invasive Cardiol. 2009 Nov;21(11):E226-8

16. Afonso L, Hari P, Mohamad T, Kondur A, Pidlaoan V, Li T, Khetarpal V, Jacob S.

Acute Myocarditis: Can novel echocardiographic techniques assist with diagnosis? Accepted European J Echocardiography 2009, Nov 24 EJE 09 227 2242

19

17.Hari PMD, Mohamad T, Kondur A, Jahania S, Afonso L. Incremental value of contrast echocardiography in the diagnosis of atrial papillary myxoma. Accepted Echocardiography 2009 ID ECHO-2009-0361

Case Reports (peer-reviewed): Posters at national Conferences and Electronic Publications

18. *Povalaram SR, Guglin M, Afonso L. Hiatal hernia Presenting as Recurrent Syncope.

Oral Presentation. Society of General Internal Medicine, 2005 Midwest Regional Meeting 2005 Chicago, IL

19. *Pitta S, Elder M, Afonso L. Quadricuspid Aortic Valve associated with Unruptured

Sinus of Valsalva Aneurysm. Valves in the Heart of the Big Apples IV: Evaluation and Management of Valvular Heart Disease 2005

20. *Pitta SR, Afonso L. Noncompaction with the Left Ventricular Myocardium with Mitral

Valve prolapse and AV Block. ACC Michigan Chapter, Feb. 2005 21. *Penugonda N, Afonso L. Complex Endocarditis in an Immunocompromised Host

Second Annual American Medical Association Resident, Fellow and Student (AMA-RFS) Research Poster Symposium Dallas, TX, Nov. 25

22. Pitta S, Kumaravelu P, Afonso L. 44-Year Old Male with Heart Failure and Continuous

Murmur. Online Case Studies. Cardiosource issue 11/22/2006 23. *Pitta SR, Vaitkevicius P, Afonso L. Extensive Circumferential Aortic Intramural

hematoma Treated with Endovascular Stenting Case of the week, Nov. 20, 2006 TCTMD-The comprehensive Cardiology and Interventional Resource, 2006

24. Afonso L, Pitta S, Vaitkevicius P. Extensive Circumferential Aortic Intramura

Hematoma Treated with Endovascular Stenting, www.tctmd.com Nov. 2006 25. *Pitta SR, Kumaravelu P, Afonso L. Unusual Physical Examination and Unusual

Diagnosis : Rare case of Aortic arch and Left Brachiocephalic Vein Fistula. Case Study Published in Cardiosource, American College of Cardiology, Nov. 22, 2006

26. *Rudraiah L, Dhabuwala J, Awad K, Afonso L. Dobutamine Induced Coronary Artery

Spasm Presenting AS ST Evaluation During Stress Echo with Normal Coronaries on Angiogram. MD. Annual Scientific Sessions Michigan ACC Chapter 2006

27. *Pitta SR, Penugonda N, Afonso L, Thatai D. A combination of pulmonary valve and

pulmonary artery stenosis in an adult with corrected Tetralogy of Fallot. Valves in the Heart of the Big Apple V, April 12-14, NYC, 2007

28. Kondur A, Pitta SR, Afonso L. Cleft Mitral valve in Adults: The role of 3-D Echo in

diagnosis and surgical planning. Valves in the Big Apple V, April 12-14, NYC, 2007

20

29. Kondur A, Afonso L. “Implantable Cardioverter Defibrillators Save Lives from

Lightening Related Electrocution too..! PACE MS #PACE-07-0190 30. *Bachour K, Awad K, Afonso L. Midventricular Ballooning Cardiomyopathy:

Epicardial spasm vs. Microvascular Dysfunction: Can Echo Identify the Culprit? Second Annual Case Competition at the 18th Annual Scientific Sessions of the American Society of Echocardiography (ASE) Seattle, WA June 16-20, 2007

Review Articles (Peer-Reviewed) 1. Afonso L, *Mohamad T, Thatai D. Crack Whips the Heart: A Comprehensive Review of

the Cardiovascular Toxicity of Cocaine . Am J Cardiol. 2007 Sept. 15;100(6):1040-3 2. Mahajan N, Khetarpal V, Afonso L. Stroke Secondary to Calcific Bicuspid Aortic

Valve: Case Report and Literature Review. Accepted to Jap Journal of Cardiology. JJCC-D-08-0094R1. Nov. 2008

3. Afonso L, *Bernal J, Bax J, Abraham TP. The Echocardiographic Assessment of hypertrophic Cardiomyopathy: Emphasis on Novel Imaging Modalities. J Am Coll Cardiol Img. 2008;1:787-800

4. *Nelson K, Li T, Afonso L. Diagnostic Approach and Role of MRI in the Assessment of Myocarditis. Cardiolo Rev. 2009 Jan-Feb 17(1):24-30

5. Khetarpal V, Mahajan N, Madhavan R, Batra S, Mopala P, Sagar A, Rapolu P, Nangia S, Afonso L. Calcific Aortic Valve and Spontaneous Embolic Stroke: A Review of Literature. J Neurol Sci. 2009 Dec 15;28(1-2);32-5. Epub 2009 Aug 26. PMID:19712938

****************************E-REVIEWS******************************* 1. *Mohamad T, Ramappa , Afonso L. Primary and Secondary Prevention of Coronary

Artery Disease. A Review. E-medicine 2006. www.emedicine.com 2. *A Kondur, Daram D, Afonso L. Arrhythmic and Mechanical Complications of Acute

Myocardial Infarction. A review. E-medicine 2006 www.emedicine.com

3. *Hanson I, Afonso L. Mitra; Regurgitation. L Review Article. E-medicine 2008. www.emedicine.com (update active 2009)

Editorials/Commentaries/Letters to the Editor

1. Dandapantula HK, Spears JK, Chandu L, Katkuri H Afonso L. Cigarette smoking and myocardial infarction in young men and women – “Let us not forget coronary vasospasm” Int J Cardiolo. 2007 Jun 25 PMID: 19594910

21

2. Dandapantula HK, Afonso L, Katkuri H, Chandu L, Spears JR. Unexplained pulmonary

hypertension in elderly patients: role of heart Failure with normal ejection fraction. Bernheim effect and reverse Bernheim effect. Chest 2007 Jun;131(6):1987-8 (intellectual content)

3. Jacob S, Agarwal K, Afonso L. QRS Fragmentation in Patients with Repaired Tetralogy of Fallot. American J of Cardiology 2009 Sep. 1;104(5):740-1

4. Afonso L, Kondur A, Hari P, Schreiber T. Findings at left heart cardiac catheterization in nonagenarians. Clin Cardiol. 2009 Nov;32(11):659

Books Authorships, Editorships, and Chapters

1. Kleerekoper K, Hisham Alrefai, Afonso L, Ramam BB “Metabolic bone disease’ in Challenging Cases in Endocrinology Humana Press 2002

2. Afonso L, Di Carli MF. Coronary Flow Regulation in Diabetes. Lanzer P and Topol E. J. Theory and Practice of Vascular Medicine; 1st ed Springe Verlag 2002

3. Afonso L, Mohamad T*, Badhaeka A*. Carditoxicity associated with drugs of abuse in Cardiovascular Toxicology, 2nd Edition. Volume 6 of the Comprehensive Toxicology Series. Elsevier. 2008 (A massive and exhaustive compilation of cardiovascular toxicity of drugs of abuse, time and labor intensive review, will be a Chapter in one of 8 Volumes I press

4. Afonso L, Bachour K*. Chapter “Assessment of LV Size and Mass” ringer, handbook of Echocardiography. *The Experts Pocket Guide to Echocardiography: The Experts Handbook of Echocardiology** A case base approach to bedside echocardiography* The Echo Pocket Guide 2009 (in press)

5. Afonso L, Mengistu S*. Prosthetic Heart Valves Springer. Handbook of Echocardiography. The Expert Pocket Guide to Echocardiography: The Experts handbook of Echocardiography A case based approach to bedside echocardiography. The Echo Pocket Guide. 2009 (in press)

6. Afonso L, Mathew A*. The Assessment of Diastolic Dysfunction. Springer, Handbook of Echocardiography. The Experts Pocket Guide to Echocardiography: The echocardiography* The Echo Pocket Guide 2009 (in press)

Abstracts (last five years)

1. *Pitta SR, Afonso L. Left-ventricular Noncompaction. Oral presentation ACC Michigan Chapter, MI 2004

22

2. *Pitta SR, Kalra N, Meri A, Afonso L. Misdiagnosis of Critical Mitral Stenosis-Poster Presentation ACP-ASIM Michigan Scientific Chapter, MI 2003

3. *Modi S, Kronenberg M, Afonso L, Chaturvedi. Gender, Carotid Stenosis and Risk Factor Treatment in African American Patients with CAD. Second International Conference on Women, Heart Disease and Stroke. American Heart Association, 2005

4. *Mohamad T, Vaitkevicius P, *Bachour K, Ibrahim M, Higassy H, Agha SD, Afonso L. Cocaine-Related Chest Pain – Utility of TIMI Risk Stratification and Effects of Beta Blockers on Outcome. Abstract published Supplement Journal American Coll Card, JACC 47 (4):288A. Feb 2006

5. *Pradhan J, *Niraj A, Afonso L. Determinants of Amount of Contrast Utilized in Patients Undergoing Percutaneous Coronary Procedure. Abstract published Catheter Cardiovasc Inter 2006;67(5):812-813

6. *Pradhan J, *Hui Jin, Afonso L. Global and Regional Contractile Abnormalities in patients with Wolff-Parkinson-White Syndrome Using Speckle Tracking Imaging. ACC Michigan Chapter Annual Scientific Sessions Oct. 2006

7. *Bachour K, Awad K, Afonso L. Predictors of Valve Perforation and Mortality in Infective Endocarditis. Poster presented at the Valvular Heart Disease: New Strategies, Mayo Clinic Conference. Las Vegas, NV 2006

8. Kondur AK, Afonso L, Vaka S, Stauffer N, Parker D, Kreighbaum K, Berenboom LD, Pimentel R, Emert E, Lakkireddy Dr. Implantable Cardioverter Defibrillators Save Lives from Lightening Related Electrocution too ….! 28 th Annual Scientific Sessions of the Heart Rhythm Society, May 2007, Denver, CO

9. Cardozo L, Afonso L, Steinberg J, Vaitkevicius P, Brown G, Checcobelli. The Value of Case Managed Tele Homecare (CMTH) in Recently Discharged Elderly Patients. Journal of the American Geriatrics Society, Volume 55, Issue s1, Page S1-S218, D-129, April 2007. Short listed for the Presidential Poster Session (top 25% abstracts)

10. *Bhandaru H, Mohammad K, Jareer F, Afonso L. Clinical Predictors of Myonecrosis in Patients Presenting with Hypertensive Emergencies. Selected by the Abstract Review Board for presentation as a poster finalist for the ACP Research Poster Competition, Internal Medicine 2007 Scientific Sessions in San Diego, CA April 19, 2007

11. *Severity of Coronary Artery Disease in Obese Patients Undergoing Coronary Angiography and Intervention: “Obesity Paradox” Revisited Abstract J Am Coll Cardiol. 2007 March 6 Supplement 1;49(():A212-13

23

12. *Rodrigues D, *Pitta SR, Afonso L. Submassive Pulmonary Embolism: To Lyse or not to Lyse? Shortlisted for Oral Presentation 30th Annual Society for General Internal Medicine Scientific Sessions, April 2007, Toronto, Canada

13. *Pradhan J, Veeranna V, Niraj A, Ramesh K, Afonso L, Schreiber TL. Racial Differences in Outcome After Percutaneous Intervention: A Five-Year Follow Up. Poster Presentation at the Society for Cardiovascular Angiography and Interventions (SCAI), 13th Annual Scientific Sessions, Orlando, FL May 2007, Catheter Cardiovasc Interv. 2007 May 1;69(6):S75-76.

14. *Terefe Y, *Pradhan J, Afonso L. Myocardial Infarction with Angiographically Normal Coronary Arteries. Poster Presentation at the Society for Cardiovascular Angiography and Interventions (SCAI), 13 th Annual Scientific Sessions, Orlando FL, May 2007 (abstract #C-37). Catheter Cardiovasc Interv Vol 2007 May I;69(6):S69

15. *Hourani R, *Hanson ID, Afonso L. Do Mitral Annular and Inflow Velocities Correlate with indices of Left Atrial Mechanical Function Derived Volumetrically by 3-Dimensional Echo? Journal American Society Echocardiography Vol 20(5);611, May 2007

16. *Hanson ID, *Venkalaya H, Mohamad T, Afonso L. Comparison of Two-Dimensional Versus Three-Dimensional Echocardiography Techniques to Measure Left Atrial Volume: Correlation with Left Ventricular Filling Pressure. Journal American Society Echocardiography Vol 205(5);567, May 2007

17. *Bandaru H, Mohammed K, *Jareer F, Afonso L. Predictors of Cardiac Troponin-I Release in Patients with Hypertensive Emergency. XIVth Scientific Sessions of the Inter-American Society for Hypertension (IASH) and the Consortium for Southeastern Hypertension Control, May 2007

18. * Niraj A, Pradhan J, Veeranna V, Afonso L. Racial Disparities in Coronary Angiography and Noninvasive Testing: Myth or Reality. 14th World Congress on Heart Disease. The International Congress on Heart Disease Annual Scientific Sessions, 2008

19. *Veeranna V, *Niraj A, Pradhan J, Afonso L. Do traditional Cardiovascular Risk Factors Predict Extent of Angiographic Stenosis in Elderly Patients. 14 th World Congress on Heart Disease. The International Congress on Heart Disease Annual Scientific Sessions, Toronto, Canada July 2008.

20. Kondur AK, Niraj A, Afonso L. Systolic Intraventicular Dyssynchrony in Hypertrophic Cardiomyopathy: Differentiation from Athlete. Heart 14th World Congress on Heart Diseases. The International Congress on Heart Disease Annual Scientific Session, Toronto, Canada July 2008

24

21. *Mathew A, *Bhandare D, Kizilbash M, Afonso L. Tei Index and Spectrum of Diastolic Abnormalities in Takotsubo Cardiomyopathy. 14 th World Congress on Heart Disease. The International Congress on Heart Disease Annual Scientific Session, Toronto, Canada July 2008

22. Afonso L, Mahajan N, Prigent F. Spectrum of Gated SPECT Myocardial Perfusion Imaging (MPI) Abnormalities in Patients with Left Main Coronary Artery Disease: A Tertiary Medical Center Experience “Journal of Nuclear Cardiology; Volume 15, Number 4;S25-31 July / August 2008. Annual American Society of Nuclear Cardiology Scientific Session ASNC Sept. 2008

23. Kondur A, Jacob S, Niraj A, Pradhan J, Sandidge G, Afonso L. Pattern and Extent of

Systolic Intraventricular Dyssynchrony in Patients with Hypertrophic Cardiomyopathy: Comparison with Hypertensive and Athlete Hearts Using Tissue Doppler Imaging. ** Selected Oral e-presentation American Heart Association . Scientific Sessions. Abst#1061. Nov 2008. New Orleans, LA. Circulation. 2008;118:s-641-642

24. Mahjan N, Mahavan R, Chaturvedi S, Afonso L. Predictors of Stroke in Patients with Low Ejection Fraction and Sinus Rhythm: Left Ventricular Non Compaction is a Risk Factor. American Heart Association’s International Stroke Conference 2009 Feb 18-20 San Diego, CA

25. Veeranna V, Niraj A,*Fakhry H, Pradhan J, Afonso L. Correlation of Lipid Profile with Angiographic Coronary Artery Disease Burden Among Elderly Patients Undergoing Coronary Angiography. American Heart Association Quality of Care and Outcomes Research in Cardiovascular Disease and Stroke Conference April 2009, Washington DC.

26. *Fakhry H, Veeranna V, Zalawadiya SK< Panaich S, Niraj A, Pradhan J, Afonso L. Coronary Artery Bypass Grafting vs. Percutaneous Coronary Intervention in Octogenarians: Impact on Clinical Outcomes. To be presented at the American Heart Association Quality of Care and Outcomes Research in Cardiovascular Disease and Stroke Conference, April 2009, Washington DC.

27. *Fakhry H, Veeranna V, Zalawadiya SK, Panaich S, Niraj A, Pradhan J, Afonso L. Detectable Troponin in Non-Acute Coronary Syndrome Patients in the Intensive Care Unit Setting. Long Term Prognosis. Society for Cardiovascular Angiography and Interventions (SCAI) 2009 Annual Scientific Sessions, May 2009, Las Vegas, NV

28. *Fakhry H, Veeranna V, *Zalawadiya S, *Niraj A, Pradhan J, Afonso L. Long Term (>3yrs) Survival Benefit for Coronary Artery Bypass Grafting vs. Percutaneous Intervention in Octogenarians: Is there Any? Accepted, Society for Cardiovascular Angiography and Interventions (SCAI) 2009 Annual Scientific Sessions, May 2009, Las Vegas, NV

29. *Badhela A, Rathod A, Kizilbash MA, Lai Z, Garg N, Bhat S, Afonso L, Jacob S. Implantable Cardiac Defibrillator Shocks Do Not Predict Death in a Secondary

25

Prevention Population. Poster Presentation at the American College of Cardiology 58th Annual Scientific Session Orlando, FL 2009. J Am Coll Cardiol. 2009;53(1) Supp 1:A131

30. Badheka A, Rathod A, Kizilbash MA, Lai Z, Garg N, Bhat S, Afonso L. Effect of Renal Disease on Recurrent Arrhythmia and Death in Survivors of Life Threatening Ventricular Tachyarrhythmias. Poster Presentation at the American College of Cardiology 58th Annual Scientific Session in Orlando, FL, 2009. J Am Coll Cardiol 2009;53(10)Supp 1:A132

31. *Badheka A, Garg N, Kizilbash MA, Bhat S, Rathod A, Jacob S, Afonso L. Incident Diabetes Mellitus in Patients with Stable Coronary Artery Disease. Incident Diabetes Mellitus in Patients with Stable Coronary Artery Disease. Poster Presentation at the American College of Cardiology 58th Annual Scientific Session in Orlando, FL, 2009. J Am Coll Cardiol 2009;53(10) Supp 1:A346

32. *Badheka A, Bhat S, Kizilbash MA, Garg N, Rathod A, Jacob S, Afonso L. Predicting Critical Peripheral Arterial Disease in Patients with Stable Coronary Artery Disease: A Simple Risk Prediction Score. Poster Presentation at the American College of Cardiology 58th Annual Scientific Session in Orlando, FL, 2009. J Am Coll Cardiolg 2009;53(1) Supp 1:A450

33. Mahajan N, *Polarvaram I, *Venkalaya H, Wang Y, Agar J, Ference B, Afonso L. Diagnostic Accuracy of Single-Photon Emission Computed Tomography Myocardial Perfusion Imaging and Stress Echocardiography for the Diagnosis of Left Main and Triple Vessel Disease: A Comparative Meta-Analysis. Accepted for the American College of Cardiology 58th Annual Scientific Session, Orlando, FL 2009

34. *Mengistu Alemayehu Simegn, Ashok Kondur, Preeti Ramappa, Tamam Mohammed, Afonso L. Strain Dispersion Index and Global Longitudinal Strain in Differentiating Hypertrophic Cardiomyopathy From Other Forms of Left Ventricular Hypertrophy. Accepted for the American College of Cardiology 58th Annual Scientific Sessions in Orlando, FL 2009

35. Mahajan N, Kondur A, Niraj A, Veeranna V, Sudhakkar R, Afonso L. Drug Eluting Stents (DES) versus Bare Metal Stents (BMS) in Octogenarians: A Comparative Outcome Analysis. Accepted for the American College of Cardiology 58th Annual Scientific Sessions in Orlando, FL. 2009

36. Mahajan N, Bhattacharya P, Madhavan R, Dunn JK, Sacks FM, Afonso L. Predictors of Cerebrovascular Disease Following Myocardial Infarction: Role of Non-HDL Cholesterol in Risk Stratification. Accepted for the American College of Cardiology 58th Annual Scientific Sessions in Orlando, FL 2009

37. Mahajan N, Kondur A, Bhat S, Kumar S, Afonso L. Impact of Renal Insufficiency on Percutaneous Coronary Interventions Outcome in Elderly: A single Tertiary Center

26

Experience. Accepted American College of Cardiology 58th Annual Scientific Sessions, Orlando, FL, 2009

38. *Apurva Badheka, Ankit Rathod, *Mohammad A Kizilbash, Tamam Mohamad, Zongshan Lai, Neha Garg, Samrat Bhat, Anil Mathew, Afonso L, Sony Jacob. Epileptic Survivors of Sudden Death Cardiac Death are at Significantly Greater Risk of Recurrent Arrhythmias and Death. 30th Annual Scientific Session of the Heart Rhythm Society 2009, Boston, MA

39. *Apurva Badheka, *Ankit Rathod, *Mohammad A. Kizilbash, *Neha Garg, Tamam Mohamad, Zongshan Lai, Samrat Bhat, Joya Ganguly, Luis Afonso, Sony Jacob. ECG Predictors of Recurrence of Life Threatening Ventricular Tachyarrythmias. 30th Annual Scientific Session of the Heart Rhythm Society, 2009, Boston, MA

40. *Apurva Badheka, *Ankit Rathod, Mohammad A. Kizilbash, Neha Garg, Tamam Mohamad, Joya Ganguly, Anil Mathew, Afonso L, Sony Jacob. Early and Consistent Lipid Lowering Therapy is Associated with Decreased mortality in Atrial Fibrillation. 30th Annual Scientific Session of the Heart Rhythm Society, 2009, Boston, MA

41. Badheka A, Rathod A, Kizilbash MA, Garg N, Mohamad T, Mathew A, Afonso L, Jacob S. An Obesity paradox in Outcomes of Atrial Fibrillation. 30th Annual Scientific Session of the Heart Rhythm Society, 2009, Boston, MA

42. *Rathod A, Badheka A, Kizilbash MA, Garg N, Mohamad T, Ganguly J, Afonso L, Jacob S. Mortality and Ischemic Stroke in Patients with Atrial Fibrillation and Heart Failure with Preserved Ejection Fraction. 30th Annual Scientific Session of the Heart Rhythm Society, 2009 Boston, MA

43. Mahajan N, *Ganguly J, *Simegn M, Bhattacharya P, Shankar L, Madhavan R, Preeti Ramappa, Afonso L. Predictors of Thromboembolic Events in Adults with Isolated Left Ventricular Non Compaction. Society for Cardiovascular Angiography and Interventions SCAI’s 32nd Annual Scientific Sessions in Las Vegas, NV, May 6-9, 2009

44. Kondur A, Mahajan N, *Sudhakar R, *Niraj A, Veeranna V, Bhat S, Afonso L. Mortality Trends of Percutaneous Coronary Interventions in Octogenarians: Bare Metal Stents vs. Drug Eluting Stents. Society for Cardiovascular Angiography and Interventions SCAI’s 32nd Annual Scientific Sessions in Las Vegas, NV, May 6-9, 2009

45. *Badheka A, *Bhat S, * Rathod A, Kizilbash M, Garg N, Mohamad T, Lai Z, Afonso L, Jacob S. Stroke Risk and CHADS2 Score For Risk Stratification In Patients with Atrial Fibrillation And Heart Failure With Preserved Ejection Fraction. Atherosclerosis, Vascular Thrombosis and Biology Scientific Sessions, AHA 2009 09-A-731-AHA-ATVB

27

46. Mahjan N, Kondur A, *Sudhakar R, *Bhat S, Afonso L. Predictors of Cardiovascular Events Following Percutaneous Coronary Intervention: Role of non HDL, Cholesterol in Risk Stratification in Octogenarians. SCAI’s 32nd Annual Scientific Sessions in Las Vegas, NV, May 6-9, 2009

47. Mahajan N, *Ganguly J, *Simegn M, Bhattacharya P, Shankar L, Madhavan R, Ramappa P, Afonso L. Echocardiographic Predictors of Thromboembolic Events in Patients with left Ventricular Non Compaction: Pulmonary Hypertension is a Risk Factor. American Society of echocardiography 20th Annual Scientific Sessions, 2009, Washington, DC.

48. *Bhadheka A, *Rathod A, *Kizilbash MA, *Garg N, Mohamad T, Jacob S, Afonso L. Left Atrial Diameter is a Predictor of Mortality in Patients with Atrial Fibrillation and Preserved Ejection Fraction. American Society of Echocardiography 20th Annual Scientific Session 2009, Washington, DC

49. *Kottam A, Afonso L. Improving Diagnostic Yield of Transeophaegeal Echocardiography for Patent Foramen Ovale: Can Fluid Bolusing Release the Door-Jam? American Society of Echocardiography 20th Annual Scientific Session, 2009, Washington, DC.

50. A Badheka, S Bhat, A Rathod, M Kizilbash, N Garg, T Mohamad, Z Lai, L Afonso, S Jacob. Stroke Risk and CHADS2 For Risk Stratification in Patients with Atrial Fibrillation and Heart Failure with Preserved Ejection Fraction. Atherosclerosis Thrombosis and Vascular Biology, AHA, Scientific Sessions, Washington, DC. 2009

51. *Mengistu Simegn, Vikas Veeranna, Kondur A, Ramappa P, Pradhan JP, Tamam Mohammad, Afonso Luis. Loss of Base to Apex Strain Gradient and Low Global Longitudinal Strain Identifies Hypertrophic Cardiomyopathy From Other Forms of Left Ventricular Hypertrophy. American Society of Echocardiography 20th Annual Scientific Sessions, 2009, Washington,DC

52. *Bhandare D, Afonso L., Jacob S. Rate of Ejection Fraction “Decay” (Delta EF) Prior to Implantation Predicts Cardiac Resynchronization Therapy American Society of Echocardiography 20th Annual Scientific Sessions, 2009, Washington, DC.

53. *Badehka A, *Rathod A, *Kizilbash MA, *Garg N, *Mohamad T, Jacob S, Afonso L. Does modality of Measurement of Ejection Fraction Matter in Survivors of Myocardial infarction with Preserved or Slightly Depressed Ejection Fraction in the Modern Era? American Society of Echocardiography 20th Annual Scientific Sessions, 2009, Washington, DC.

54. Veeranna V, Niraj A, Fakhry H, Pradhan J, Afonso L. Correlation of Lipid Profile and Angiographic Coronary Artery Disease Burden Among Elderly patients Undergoing Coronary. Angiography Society for Cardiovascular Angiography & Interventions (SCAI) 32nd Annual Scientific Sessions, Las Vegas, NV, May 6-9, 2009

28

55. Rathod A, Badheka A, Kizilbash MA, Garg N, Veeranna V, Mohamad T, Jacob S, Afonso L. Abstract 3148: Development of Heart Failure is a Risk Factor for Incident new Onset Diabetes in the Community. Oral Presentation, Circulation, Nov. 2009;120:S749.

56. Veeranna V, Niraj A, Zalawadiya S, Pradhan J, Afonso L. Alcohol Consumption and Cardiovascular Risk Among United State Adults: An Analysis From the US National Health and Nutrition Examinations Survey (1999-2006), Circulation, Nov. 2009;120:S487

57. Mahajan N, Sarsam S, Mopala P, Polavaram L, Venkalaya H, Wang Y, Agar J, Ference B, Afonso L. Exercise Single Photon Emission Computed Tomography (SPECT) Imaging or Exercise Echocardiography? A Meta-Analysis of Exercise Testing for Diagnosis of High-Risk Coronary Artery Disease. Circulation, Nov. 2009, ;120:S369.

58. Bernard D, Pinheiro A, Bhandare D, Dimaano VL, Nazarian S, Afonso L, George R, Thompson R, Brenner J, and Abraham TP. Electrocardiography versus Echocardiography in High-Volume, Community-Based Screening for Potentially Life-Threatening Cardiac Pathologies in Athletes: Implications for Optimal Screening Strategies, Circulation, Nov. 2009;120:S410-S411.

59. Rathod A, Badheka A, Kizilbash M, Garg N, Veeranna V, Mohamad T, Jacob S, Afonso L. Development of Heart Failure is a Risk Factor for Incident New Onset Diabetes in the Community. Oral Presentation. American Heart Association, Scientific Session. Nov. 2009, Orlando, FL

60. Rathod A, Veeranna V, Manickam P, Badheka A, Niraj A, Pradhan J, Jacob S, Afonso L. Effect of Lipid Subclasses on The Risk of Cardiovascular Outcomes in An Ethnically Diverse Asymptomatic Population. American College of Cardiology 59th Scientific Session, March 14-16, 2010 in Atlanta, GA

61. Rathod A, Badheka A, Manickam P, Veeranna V, Sudhakar R, Mohamad T, Jacob S, Afonso L. Progression of Atherosclerosis in an Asymptomatic Population-Effect of Lipid Subclass. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010 in Atlanta, GA

62. Zalawadiya SK, Pradhan J, Niraj A, Veeranna V, Manickam P, Hari PK, Sony J, Afonso L. Waist Hip Ratio, Not Body Mass Index Predicted Cardiovascular Risk in a Multi Ethnic Cohort of Healthy Adults. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

63. Zalawadiya SK, Niraj A, Veeranna V, Pradhan J, Panaich S, Sony J, Afonso L. Is There a Gender Disparity in Risk Association of Inflammatory Biomarkers with New Onset Diabetes in Healthy Adult Population? American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010 , in Atlanta, GA

29

64. Manickam P, Rathod A, Badheka A, Hari PK, Veeranna V, Pradhan J, Jacob S, Afonso L. Prognostic implications of Atherogenic/Anti-Atherogenic Lipid Rations in an Ethnically Diverse Population. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA.

65. Manickam P, Rathod A, Badheka A, Hari PK, Veeranna V, Pradhan J, Jacob S, Afonso L. Disparities in Association of Various Lipid Ratios on the Location and Severity of Atherosclerosis in Different Vascular Beds. American College of Cardiology 59th Annual Scientific Sessions, March 14-16, 2010, in Atlanta, GA

66. Manickam P, Veeranna V, Rathod A, Hari PK, Zalawadiya S, Badheka A, Jacob S, Afonso L. Novel Biomarkers Associated with Left Ventricular Hypertrophy. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

67. Hari PK, Veeranna V, Manickam P, Zalawadiya S, Mengistu S, Pradhan J, Jacob S, Afonso L. Microalbuminuria in Non-Diabetic Patient with Metabolic Syndromes is a Strong Predictor of Carotid Artery Distensibility and Thickness. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

68. Hari PK, Veeranna V, Sudhakar R, Manickam P, Niraj A, Kaur R, Jacob S, Afonso L. Gender and Ethnic Differences in Cardiovascular Events and Mortality in Patients with Depressive Symptoms. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

69. Hari PK, Veeranna V, Manickam P, Sudhakar R, Simegn M, Zalawadiya S, Jacob S, Afonso L. Microalbuminuria in Non-Diabetic Patients with metabolic Syndrome is Strongly Associated with Systemic Inflammation. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

70. Hari PK, Veeranna V, Sudhakar R, Manickam P, Pradhan J, Jacob S, Afonso L. Microalbuminuria in Non-Diabetic Patients with Metabolic Syndrome is a Strong Predictor of Subclinical Atherosclerosis: A Multi-Ethnic Perspective. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

71. Hari PK, Veeranna V, Sudhakar R, Manickam P, Kaur R, Pradhan J, Jacob S, Afonso L. Does the Presence of Microalbuminuria Portend a Worse Outcome in Non-Diabetic Patients with Metabolic Syndrome? American College of Cardiology 59th Annual Scientific Session, March14-16, 2010, in Atlanta, GA

72. Veeranna V, Niraj A, Zalawadiya S, Pradhan J, Jacob S, Afonso L. Gender Difference in Novel Biomarkers for Predicting Future Cardiovascular and Coronary Events. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA.

30

73. Mahajan N, Sagar A, Khetarpal V, Kondur A, Sarsam S, Polavaram L, Venkalaya H, Wang Y, Agar J, Afonso L. Pharmacologic Single Photon Emission Computed Tomography (SPECT) Imaging or Pharmacologic Echocardiography? A Meta Analysis of Pharmacologic Testing for Diagnosis of High Risk Coronary Artery Disease. American College of Cardiology 59th Annual Scientific Session, March 14-16, 2010, in Atlanta, GA

INVITED LECTURES/PRESENTATIONS

1. Invite Expert: Michigan Society of Echo, Stump the Stars. Regional Chapter Meeting, Aug 2005

2. Diastolic Heart Failure: Feeling or Filling out of Sorts. Michigan Chapter. American

College of Cardiology Scientific Meeting, Oct. 2005 Traverse City, MI

3. Update on ECG Interpretation, (Clinical Skills Development Session). Michigan Chapter, American College of Physicians, Scientific Meeting, Sept. 2005. Also served as judge for Poster Competition, Michigan Chapter and American College of Physicians, Scientific Meeting Sept. 2005

4. Cardiac Resynchronization Therapy. Pathophusiologic insights and identification of responders. Congestive Heart Failure. New Frontiers in Cardiology 2nd Annual Conference organized by Providence Hospital, Westin Hotel, Southfield, MI Sept. 21, 2006

5. Current Status of 3 D Echocardiography: Overview with Illustrative Examples Michigan Society of Echocardiography, Annual Conference, Crystal Mountain, MI Feb 8th 2009

6. Strain Imaging: Principles to Practice. Michigan Society of Echocardiography, Annual Conference. Crystal Mountain, MI Feb 8th 2009

7. The Echocardiographic Assessment of Prosthetic Heart Valves Michigan Society of Echocardiography, Spring Meeting May 2009. Kresge Auditorium Harper Hospital, Detroit MI

Invited Seminars and Grand Rounds

1. “Tissue Doppler Imaging: Contemporary Applications, 2006. Invited lecture to Cardiology Section of the Perinatology Research Branch NIH, Wayne State University, Detroit, MI

2. Recent Advances in Echocardiography, Medical School Grand Rounds, Nov. 15, 2006. Scott Hall Wayne State University Medical School, Detroit, MI

31

3. Peripartum Cardiomyopathy: Focus on Etipathogenesis and Diagnosis. Perinatology Research Branch, National Institute of Health, Harper University Hospital, Detroit, MI, Aug. 2008