KHOA HOC CONG NGHE

rXlGHIEIV c u u T H U SIIVH K H O I , Y E A S T EXTRACT V A

-GLUCAIM T i i IVAIVl MEIXI SACCHAROMYCES CEREViSSAE

M U d i TROIVG M d i TRUdlXIG IVUQC C H U A T A U H U

Nguyto Ngpc ThanhS Ding Thanh Nhin\

Trin Thi Hong VanS Biu Hoang Dang LongS Hu:jmh Xuin Phong^

T6MTAT Muc U u ctia nghi&n cuu nhSm tuy^n chon chiing nam men Saccbaromyces cerevisiae co kha nilng tang sinh khoi cao, xac dinh di&u kien thich hpp cho DEim men tang sinh khoi trong moi truong nuoc chua tau hu va khao sat di&u kien thu nhan yeast extract va [)-glucan. Ket qua da tuyen chpn duoc chung S. cerevisiae LXl.l cho smh khoi dat 0.204 g/100 mL trong moi tnrong mroc chua tau hu. Nghien cuu cac y^u to anh hudng d#n kha nang san xual smh khdi dua tren mo hmh Plackett-Burman (PBD) vm 7 nhan to khao sat. Kel qua da xac dinh duac 3 yeu to (pH, mat so va ham lirong peptone) co y nghia va duoc chpn xac dinh di u kien loi mi theo bd tri Central Composite Design (CCD). Ket qua cho thay chung S. cerevisiae LXl.l nuoi ciiy trong di u kien vdi mat sd 10' te bao/mL, ham tuong peptone 4,78 g/L va pH 5,59 cho sinh khoi dat 0.469 g/100 mL Klidi luong yeast extract thu hoi dal 0.298 g va 2.583 g p-glucan tir 3,0 g sinh khoi kho te bao nam men, tuong ung hieu suat thu hoi p-glucan la 86,10%. Tu khda: IJ-glucan. nude chua lau hu. Saccharomyces cerevisiae. yeast extract.

I.BATVANOE

Nam men la vi sinh vat dupc biet den tir rat sdm va nd dupc ung dung rpng rai trong sin xuat va ddi sdng. Nhieu cdng trinh nghidn cuu ve nim men dupc Ihuc hien vi mang lai nhi^u thanh tuu dang k^ nhu lao vaccine viem gan A, msuhn, interferon tir nam men tii to hpp. tao ra nam men bia cd Itha nang k^t Idng cao,... (Ferreit-a et al, 2010; Ferreira et al, 2016). Ngay nay, nim men ddng vai trd quan trong trong nhidu linh vuc nhu cdng nghe thuc pham, cdng nghe l^n men, y hoc,... (Luong Due Pham. 2009). Dac biet, nam men duoc ung dung rat nhi^u trong san xuit protein don bao bdi te bao nam men chiia ham lupng protein cao O^ hoang 50-60% theo chit khd) vi nguon vitamin phong phu. Chinh vi thd nd dupc su dung nhu ngudn dinh dudng cho con ngudi. ddng vat va dac biet lam mdi tiirdng dtnh dudng cho iihi^u vi sinh vit khac (Suman et al, 2015: Ritala et a/, 2017).

Hien nay, cdng nghe sin xuat sinh khdi nam men vi cic sin pham tir vi sinh vat vdi muc tieu giim chi phi sin xuat cung nhu nang cao tinh canh tranh ciia san pham ndn nhidu nghien ciiu da tan dung

' \ len Nghien ciru \ a Phat trien Cong nghe Sinh hoc. Trudng Dai lipc Can Tho Email: hxphong@clu.edu.vn

nhirng ngudn phu pham nhu ri dmmg, ba rupu. ba bia, dich thiiy phin nguyen lieu lignocellulose,...(El-Baki-y ei at., 2015: Muniraj ei a!., 2015; Spalvins ei ui, 2018). Tiu hu hay cdn goi li diu hu, la mdt sin pham giau gii tri dinh dudng, dd chd bidn va cd gia thinh re nen duoc su dung phd bien. Nude chua tau hu chua cac thinh phan dinh dudng va protein ngudn gdc thuc vit, trong do bao gdm isoflavon, ohgosacchande va peptide vdn tuong dong cac thanh phan protein, dudng va dmh duong cd trong mdi tru'dug nuoi cay vi smh vit (Sanjay ei a!., 2008). Theo Trin Vu Phuong (2011), nude chua tiu hu chu^ thanh phan chat khd 16,1 g/L, trong dd thanh phin nito tdng sdla l,37g/Lva nito formol la 0,25 g/L Do dd, ngudn nguyen heu nay da dupc ung dung trong mdt sd nghien ctiu de nudi cay tang smh khdi vi khuan lactic (Jhi et al, 2003; Ounis ei ai, 2008). Theo nghien cuu cua Smtawarrdani (2011). hang nam cd khoang 20 trieu m'' nude chua tiu hii duoc thai ra tir 2,56 trieu tin dau nanh trong cdng nghiep sin xuat tau hu b Indonesia, tuong duong vdi 1 kg dau ninh duoc san xuat tao ra gin 8 lit nude chua va thai ra 1 kg COo. Nude chua tau hu la mdt ngudn gay d nhi6m mdi truong dupc thai ra tir qua trinh san xuat tau hu nen hudng nghien cuu nay cung nham phin nio han che d nhiem mdi tnrdng.

Yeast extract bao gdm cac thanh phan hoa tan cua td bio nam men vi duoc ung dung rdng rai trong

NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2019 89

KHOA HOC C 6 N G N G H |

nganh cdng nghi?p thuc phim nhu huong heu thuc pham. phu gia va vitamm, kha nang lam tron vi. giim mili kho chiu. khdng gay di img. an toin. dd bao quin vi stt dung,... (Suman etal., 2015; Podpora et al., 2015; Podpora et al.. 2016). ^glucan dupc xem la mdt trong nhiing thuc pham bd sung cung cap chat dmh duong ddng thdi dap ung tinh an toin cho ngudi sir dung, p-glucan li mdt chat cd boat tinh sinh hoc cao, nd hd trg diet te bio ung thu, cac benh dj tmg, phdng ngira nhidm trung dudng hd hap. giam long luong cholesterol vi cic hpid miu (Behall et al.. 2004; AbuMweis e/a/, 2010; Jesenak ef a/, 2017).... Vi viy, viec nghien cuu va sin xuit p-glucan ngay cing dupc cac nha khoa hpc chu trpng.

Nghien ciiu niy dupc thuc hien nhim muc dich su dung nude chua tiu hu mpt cich cd hieu qua, giam chi phi sin xuat ddng thdi gdp phin ban che d nhidm mdi tnrdng de nudi tang sinh khdi nam men. Qua dd, xac dinh cic dieu kien thich hpp de thu hdi yeast exhact va p-glucan tir sinh khdi nam men. la nhimg san pham cd gia tri dinh dudng va gia tn kinh td cao.

Z. PHUDNG PHAP NGHCU CUU

2.L Nguyen v§th$u

Mudi chiing nam men Saccbaromyces cerevisiae dupc luu tru d Phdng thi nghidm Cdng nghe Sinh hpc Thirc pham, Vidn NCt&PT Cdng nghe Sinh hpc, Trudng Dai hpc Can Tho. Man nude chua tau hu dupc thu tai CO sd san xuat tuong chao \'inh Tran (hem 42, dudng Trin Viet Chiu, phudng An Hda, quin Ninh Kidu, tiiinh phd Cin Tho) cho mdi dot thir nghiem. Mdi tnrdng tang sinh nim men YPD long (peptone 1%, yeast extract 2%, D-glucose 2%) (Limtong et al.. 2007) va mpt sd hda chit tinh khiet nhu NaOH. dietylete, NaHSOj... la cic sin phim thuong mai cua Cdng ty Merck (Dire). Sigma-Aldrich ff)irc) va Hi-media (An Bo).

2.2. Khio sit thinh phan nguydn H§u mrdc chua t iuhu

Miu nude chua tau hu sau khi thu dircrc tij co so se duoc ipc lai bing giiy lpc (dudng kinh Id 11 pm). Miu nude chua sau lpc duoc khir tnmg d 121 C Sau khi khir tiling, miu nude chua duoc gui phan tich kdt qui cac chi tieu (ham luong acid tdng, chat beo. dudng khir. protein/nito tdng sd vi p-o tdng/khoing tdng) lai Trung tim Kiem soat benh tit thinh phd Can Tho (Bang 1). Chi tieu pH dupc do tivc tiep tai

phdng thi nghidm bang thiet bi do pH j i '- 'v 3510

(Cole Parmer, Vuong qudc Anh).

Bang 1. Cic chi tieu phin tich thinh phan nude chua

tiu ha

STT

1

2

3

4

5

6

Chi lieu

Ham lugng acid

Ham lirgng chat beo lipid

Ham lirgng ducmg khu

Ham lugng protein/ Nita tong so

Ham lugng tro tong so

pH

Don VI tinh

So mL NaOH IN/

100 mL

g/100 mL

g/100 mL

g/100 niL

g/100 mL

Phuxjng

phap kiem

TCVN 4073:2009

TCVN 4592-1988

TCVN 6960-2001

AOAC 99120-2000

FA019B6 14/7, P228

Do bang pH Jenway 3510

90 NONGNGHIEPVA PHAT

2.3. Tuy^n chpn nam men c6 kha nSng cho sinh kh6icao

Muc dich nhim xac dinh chung nam men c6 kha nang san xuat sinh khoi cao nhat trong moi trucmg YPD va nuoc chua tau hu. Thi nghiem dugc bo W hoan toan ngSu nhien voi 1 nhan td (10 chung nam men) va 3 lin lap lai. Mgt mL dich nam men (mat so 10» te bao/mL) dugc chiing vao 99 mL moi truang YPD broth hoac nuoc chua tau hu (da dugc khir UTing 12rC trong 15 phiit). UlSc 150 vong/phiit o nhiet do 30-C trong 48 gig. Sinh khoi nam men dugc loc va say o 45-C den khi kh6i lugng khong d6i, can xac dinh khdi lugng sinh khdi theo g/100 mL mdi trucmg long.

2.4. Khao sat cic ydu td anh huong ddn khi nSng tang sinh khdi cua nam men

Mvc.dich nham xac dmh cac nhan td anh huong den kha nang lang smh khdi cua n i n men trong mo, truong nude chua tau hu. Sir dung md hinh thu nghiem Plackett-Burman t„ u' ' n - tr .^ n „ ,^ '^"""an tu phan mem Design Expen 7.0 (Slat-Ease Inc., USA). Cac nhan

I N ^ HCl o ' , r ^ " * " " * ' " ^ ^™*.' NaOH 0,1N va HCl 0,1N), mat so g.ong ehu„g ban diu ham lugng dugng, peptone, ammoniL - , . txu Mn \ ™ ™ • , """nonium nitrate (NH.NOJ, magnesium sulfate (MgSO 7H nl potassium dihydrogen phosphate (KH Pn'^ , . -nhan Id du,;c thiet ke d 2 muc do c iT u ' '•

• ^'^ nhan td thu ' " mfc do dugc

TRliN NONG THON-KY 2-Th NG n / j g , ,

KHOA HOC CONG NGHE

the hi?n b bang 2. Cac buoc thuc hien thi nghiem dupc tien hanh tuong tu nhu tren vi mdi nghiem

thuc dupc thuc hien 3 lin lap lai.

Ky hidu

A B C D E F G

Bing 2. Cic nhin td thii nghidm vi ndng

Nhantd

pH Mat sd gidng chiing Ham lugng duong Peptone Ammonium nih-ate (NHjNO.i) Magnesium sulfate (MgS0,.7H.,0) Potassium dihydrogen phosphate (KHaPO J

dd tuong ling vdi hai mijrc do

Donvi

tb/mL

g/L g/L g/L g/L g/L

Miic thap (-1)

4 1x10=

18 0 0 0 0

Miic cao (-1-1)

6 1x10'

25 6,0 1,5 2,0 2,0

2.5. Nghien ciiu tdi mi hda theo bd tri CCD

Su dung mo hinh thu nghiem Central Composite Design (CCD) tir phin mdm Design Expert 7.0 de xac dinh cac dieu kidn tdi uu cho qui trinh nudi sinh khdi nam men tir nude chua tiu hu. Su dung cic nhan td cd tic dpng y nghia den qua tiinh len men tir k^t qua thir nghiem PBD vi mdi nhan td dupc thiet k^ vdi 5 muc dp (-a; -1,0; 0; 1,0; +a). Md hinh tdi uu hoa theo CCD dupc bd tri ldn men trong 100 mL b 30'C trong 48 gid. Tir ket qua thuc nghiem tdi uu hda s6 chpn cac didu kidn tdi uu de tien hanh thu nghiem xac nhin kdt qua.

2.6. Khio s i t anh hudng ciia nhidt dd vi thdi gian dhi qui trinh ti^ phin nim men

Nam men se dupc tir phin dudi tic dung cua cic enzynie ndi bio a 3 muc nhiet do (45, 50, 55°C) va 3 khoang thai gian (24, 48, 72 gid). Phin dung dich dupc thu sau ly tam dich tu phan dupc say khd dd tliu yeast exti-act. Phuong phip thuc hien nhu sau: Can 3,0 g sinh khdi khd te bao nam men cho vio dia petri, them 20 mL nude cat, trdn ddu va u d cic mirc nhiet dp 45X, 50X vi 55T. Sau thdi gian 24 gid, 48 gid va 72 gid, ly tim a 4.000 vdng/phut ti'ong 10 phiit. thu phan dich. cho vao dia peti-i vi say d 45T den khi khdi lupng klidng ddi. Cin de xac dmh khdi lupng yeast exti-act thu hdi.

2.7. TTiu hdi p-glucan trong nam men S. cerevisiae dupc nudi ciy bing nude chua tau hu

Quy trinh xir ly phin vd te bao nam men de thu hoi p-glucan dupc thuc hien theo md ta cua Pham Viet Cudng va cdng su (2005). U phan can tim duoc sau khi ly tain djch tu phin nim men vdi NaOH 3,3% (w/v) ti-ong 18 gid d 75X. De ngudi de'n 40°C, bo sung nude cat, lac ddu va ly tim d 4.000 vdng/phut h-ong ID phiit, tiiu phin cin. Bd sung NaOH 3,3% va khu^y ddu d 75T trong 1 gid. De ngudi ddn 30X. bo

NONG NGHIEP VA PHAT TRIEN NONG THON

sung nude cit vdi ti le 1:1 (v/v), ly tim d 4.000 vdng/phut trong 10 phut, thu phin can (vd td bio nam men). Chuyen phan vd td bio nam men thu dupc vao binh tam giic 250 mL, thdm vao 100 mL NaOH d cac ndng dp 2,0%; 2,5%; 3,0% va 3,5%; khuiy ddu d 75X trong 2 gid. Ly tam d 4.000 vdng/phut trong 10 phut, thu phan can. Them 20 mL cdn tuydt ddi, khuay deu va de yen trong 10 phut, ly tim d 4.000 vdng/phut trong 10 phut, thu phin cin, lip lai bude niy 2 lin. Them 20 mL dietylete, khuay ddu va de yen trong 10 phiit, ly tim d 4.000 vdng/phut trong 10 phut. Thu phin can vi say khd d 45X trong 48 gid. Can xac dinh him lupng p-glucan thu hdi vi tinh hieu suit thu hoi tii 3,0 g sinh khdi khd te bao nam men.

2.8. Phan tich sd Hdu vi thdng kd

Ket qua thi nghiem dupc xir ly bang phin mdm Microsoft Excel 2010 (Microsoft Corporation, USA). Cac sd heu duoe xu ly thdng ke bing phin mdm Statgrahics Centurion XV (Statgraphics Technologies. Inc.. USA) va Design Expert 7.0 (Slat-Ease Inc., USA).

3. KET QUA VA THAO LUAN

3.1. Phan tich thinh phin nguydn li§u nude chua tau ho

Ket qua phan tieh eic thanh phin hda ly co ban cua nude chua tau hu dugc trmh bay d bang 3. Bang ket qua cung trinh bay so sanh thanh phin dinh dudng ciia nude ehua tau hu vdi mdt sd ngudn phu pham ti-ong san xuit cdng nghiep. Nhin ehung, ham lupng dudng khu cua nude chua tiu hu thip hon so vdi ri dudng mia. siro cao luong va siro bip. Tuy nhien, nude chua tiu hu lai chiia ham luong protem long cao hon cic nguyen heu edn lai vdi 0,37 g/100 mL nguyen heu. Theo Takara et al (2007), cic thanh phan suo va ri dudng chira cie hpp chat phenol hinh thanh do phan ting Maillard dudng gay lic ehe su

-KY 2-THANG 11/2019 9!

KHOA Hpc CdNG NGHt

phat tiien vi sinh vat N mic ehua tau hu khdng xiy ra che cie nhdm nam men khi nudi cay. Tu cac chi tidu phan ling Maillard do ham luong dudng thap nen ti-en cho thiy tidm nang nudi cay nim men cua nude khdng chiia cac hop chit phenol vi vay khdng gay lic chua tau hu.

Bang 3. Inanh ptian

Loai nguyen lieu

Nude chua tau hu'' Nude chua tau hu'' Ri duong mia' Siro cao luong"' Siro bdp''

mptsc

pH

5,31 5,27

loai phu pham

Ham Iuong acid (g/100

mL)

0,62

17,50 0

cd tiem nflng ling dung trong Idr

Ham lutmg chdt beo hpid (g/100 mL)

<0,1

0.1 0 0

Ham luong dudng khii (g/100 mL)

0,50 1,61

74,72 74,90 77,59

men so vdi nudi Ham luong

Protein/nitu tdngsd

(g/100 mL)

0.37 1,82

0 0 0

chua tau hu

Hani lupng tro tdng sd (g/100 mL)

0,51

3,3

2,0

Ghi chu: ^Ket qua phan tich cua Tnmg tam Kiem soat benh tat TP. Can Tha, 2019: ''Tran Vu Phuong. 2011:' Vidra et al. 2017: HIS Department of Agriculture, 2019; -: khdng xac dinh.

3.2. Tuy^n chpn nim men cd kha ning cho sinh

kh^icao

Bing 4. Khdi lupng sinh khoi khd cua cac ddng nim men nudi ii trong mdi trudng YPD long va mdi

tnrdng mrdc chua tiu hu

Ddng nam men

CTl.l CM2.1 HG2.1 ST1.3 BL2.3 1A1.3 DT2.3 KG3.1 HG1.2 LXl.l CV(%)

Mdi trudng YPD Broth (g/100 mL)

0,138' 0,152' 0,153' 0,167' 0,168' 0.175' 0,239^ 0.383"'' 0,456-' 0,449"

14,172%

Moi trudng nude chua tau hu ' (g/100 mL)

0,132' 0.166'"

o.ns''" ; 0,174''-0,151'" : 0.182"'' 0,153'«- \ 0,161'" 0,149'"' 0.204' 13.39

Ghi chu: cac gia tri trong bang li gii tri trung binh aia 3 lin lap lai. Cac gia tri trong cdng mdt cot cd mau tu gidng nhau thi khac biet khong coy nghia thdngke a muc.•>% (P<0.05).

Ket qua khao sat kha nang san xuat smh khdi ciia 10 ddng ndm men .S'. cerevisiae trong mdi truong YPD long va moi trudng nude chua lau hu dupc tnnh bay d bang 4. Kel qua cho thay cd su khac biet ve kha nang san xuat sinh khdi giira 10 ddng nam men. Sau 48 gid nudi ciiy. luong sinh khdi cua 10 ddng nam men tren moi trudng YPD long trong khoang 0.138-0.449 g/100 mL, 3 chiing cd luong smh khdi

cao khac biet y nghia thdng ke vdi do tin cay 95% so vdi 7 chung cdn lai ldn luot la S. cerevisiae HG1.2, LXl.l, KG3.1 (0,456 g; 0,449 g va 0,383 g/100 mL). Trong mdi trudng nude chua tau hu, cdc chiing nam men cho sinh khdi cao ldn lupt la LXl.l, LA1.3, ST1.3, HG2.1 (0,204 g; 0,182 g; 0,174 g va 0,173 g/100 mL), trong dd thi ddng nam men LXl.l cho sinh khdi cao nhdt.

Nhin chung, khdi lupng sinh khdi khd thu dupc tir moi trudng YPD long (0,138-0,449 g/100 mL) cao hon moi trudng nude chua tdu hu (0,132-0,204). Kdt qua nay cho thdy thanh phdn dinh duong ddp ling cho nhu cdu su dung cua ndm men trong nude chua tau hu thdp hon moi trudng YPD Idng, thanh phan prptein tdng chi 3,7 g/L (Bang 3) so vdi 10 g/L peptone va 20 g/L yeast extract trong mdi trudng YPD; ham lupng dudng khu tong nude chua ldu hu chi 5,0 g/L trong khi trong moi trudng YPD la 20 g /L Ket qua nghien cini cua Duong Anh Nguyet (2012), chiing S. cerevisiae Y0904 cd kha nang cho sinh khoi cao 0,56 g/100 mL trong mdi trudng ri duong vol nong do duong 6%, pH 4,5 cd bd sung 15 g/L urea va 15 g/l, DAP. Ben canh dd, cd thd thdy kha nang thu smh khdi nam men trong mdi trudng ri duong cao hon moi hTiOTirnmirnh,,,,' i . - j u-, , „ . , *• """^ ctiua tau hu do ham luong duong trong moi Uuw,j, „jjj de gia tang luong sinh kho: nam n. nude chua tau hu, vi^c khao s,(! ddn khd nang san xudt sinh i , .,i, ; mdi truong nude chua tau hu thi nghiem Udp theo.

Trong moi truong Y'Pb \u: cerevisiae LXl.l va HGI 2

ao hon. Vi vay, I ' 0 moi trudng *'•' -" .uh hudng "am men trong J^n hanh d cac

"'•'-'' •'=' Chung S. "•" '• ''i"h khdi khac

NONG NGHIEP VA PHAT TRIEN NONG THON - KV 2 - ^NGli /20I9

KHOA HQC CONG NGHE

bi^t khdng cd y nghia thdng ke Oan lupt l i 0,449 g va 0.456 g/100 mL). Hon nira. chung 5. cerevisiae IXl.l cd lupng sinh khdi li cao nhat vi khac biet y nghia vdi chiing S. cerevisiae HGI.2. Vdi muc tieu tin dung ngudn nude chua tau hu dd nudi sinh khdi nam men ndn chiing .S' cerevisiae LXl.l li lua chpn phii hop nhit cho cic nghien cuu tidp theo.

3.3. Cic y^u td anh hudng ddn kh i nang ting sinh khdi cua n ^ men

Ket qua thi nghiem sang loc cic yeu td cd tic dpng y nghia den smh khdi nam men bing thiet ke

Plackett-Burman voi 12 nghiem thuc b bang 5. Lupng sinh khdi nim men dat trong khoang 0.165-0.4(52 g/100 mL so vdi lupng sinh khdi do md hinh du doan trong khoang 0.11-0.40 g/100 mL Ket qui cho tiiay nim men eho sinh khdi cao nhat d Iin luot cac nghiem tiuic 3, 10, 11 (0.462 g, 0,447 g vi 0.428 g/100 mL) vi cho smh khdi tiiip nhat d nghiem tinic 4 (0,165 g/100 mL).

Nghiem Ihiic

1

2 3 4

5

6 7

8

9 10

11

12

pH

6,0 4,0

6,0 4.0

4,0

4,0 6,0

6,0

4,0

6,0 6,0

4,0

Bdng 5

Matsd (tb/mL)

Ixio '

Ixio' 1x10"

IxlO"

Ixio'

Ixio' Ixjo'

Ixio '

jxio '

IxlO" Ixio ' Ixio'

Kdt qua kha Ham lupng

dudng (g/L) 18,0

25,0 25,0

18,0

25,0 18,0

18,0

18,0 25,0

25,0 18,0

25,0

0 s^t cdc nhan td anh hudng theo md hinh PBD

Peptone (g/L)

6,0

0,0

6,0 6,0

0,0 6,0

0,0

0,0

0.0 6,0

6,0 0,0

NHjNOj (g/L)

1,5

1,5 0,0

1,5

1,5 0,0 1,5

0,0 0,0

0,0

1,5 0,0

MgS0,.7 H,0 (g/L)

2.0 2.0

2,0 0,0 2,0

2,0 0,0

2,0

0,0 0,0 0,0

0,0

KHjPO, (g/L)

0,0

2.0 0,0

0.0 2,0 2.0

2,0 0,0

0,0 2.0

2.0 0,0

Smh khdi ly tiiuyet

(g/100 mL) 0.19 0,11

0,36 0,19 0,27 0,21

0,33 0,21

0,28 0,40

0,40 0,40

Sinh khdi thuc le

(g/ 100 mL) 0,208 0,191

0,428 0,165 0,197

0,175 0,272 0,223

0,227 0,447

0,462 0,359

Ghi chd- gia tii ti'ong bang la tivng binh cua 3 ldn lap lai, trong cung mdt cdt cac chir sd mu gidng nhau thi khac biet khdng cd y nghia vi mat thdng ke 5%(P<0,05).

Bang 6. Kdt qui phan tich ANOVA

Ngudn bien thien

Md hinh A-pH

B-Mat sd C-Dudng

D-Peptone E-NHjNO.,

F-MgSO, G-KH,PO, Sai sd chuan Tdng

Tdng binh phuong

0,160 0,097 0,020 10,549 0,038 21.937

3.605 0,679 0,010 0,170

Bathrdo(dt)

7 1 1 1 1 1 1 1 4 11

Sal sd chudn

0.023 0,097 0,020 10,549 0,038 21,937 3,605 0,679

R-

Gia In F

9,180 38,37 8,090 1,550 14,970 0,320 14,488 0,980

0,9414

Gia In P

0,025 0,004 0,045 0,282 0,018 0,601 0,945 0,378

Ket qua xu ly thdng ke bing phan mdm Design Expert 7.0 cho thay md hinh cd y nghia thdng ke vdi gia tin P = 0.025 < 0.05 va R- = 94,14% (Bang 6). Nude chua tiu hu cd chira ham lupng dudng khu khoang 5

g/L vi mpt sd loai mudi klioang nhit dinh (the hien long lupng ti-o 0,51 g/L) dii dap ling cho nhu cau phat trien ciia nim men nen cac yeu id nay khdng cd y nghia thdng ke (P > 0,05). Mat sd gidng chung ban

NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11/2019 93

KHOA HOC C 6 N G NGHfi

diu va pH mdi iruong la hai nhin td cd tie ddng y nghia den lupng smh khdi hinh thinh vdi gii tin P Iin lupt la 0,045 va 0,004. Ham lupng dam. mit sd gidng chiing vi pH li nhiing nhan td cd tac ddng y nghia khdng chi ddn kha nang tang sinh khdi ma edn inh hming rit ldn ddn boat ddng ti^o ddi chit ciia nim men da dupc chting mmh trong nhidu nghien ciiu da cdng bd (Di Seno et al.. 2003; Adoki. 2008; Peria et a/, 2015).

Tir ket qui thdng ke. cie nhin td cd tac dong cd y nghia den sinh khdi nim men thdng qua md hinh PBD dugc xac dmh bao gdm pH. mat sd gidng chung, him lupng peptone bd sung vdi cac gii tii P lan lupt la 0.004; 0.045 va 0.108. Do dd. cic nhan td nay tiep luc duoc thii nghiem tdi uu hda thdng qua mdhinh CCD.

3.4. Tdi uu hda theo bd tri CCD

TLT kdl qua thi nghiem tren. 3 yeu td duoe lua chpn d^ tidn hinh tdi uu hda dua tren md hinh tiiuc nghidm CCD d^ xac dinh dieu kien sin xuat sinh khdi nam men trong mdi trudng nude chua liu hu. Kdl qua so sinh khdi lupng smh khdi khd thu dupe tir tiiuc nghipm (0.102-0,469 g/100 mL) vdi gia tin du

doan dua ti-en md hinh (0,100^.440 g/100 mL)

Bing 7. Md hinh thuc nghidm vi k^t qua tinic nghidm

khdng cd su chenh lech ding ke (Bin.c ' '^ieu ki^n cho khdi lugng smh khdi cao nhal o ian lupt cic nghiem tiiiic 19 (0,469 g/100 mL). \S t0.420 g/100 mL). 12 (0,414 g/100 mL), 16 (0.410 g/100 mL) va cho sinh khdi thap nhat li b nghiem thirc 11 (0,102 g/100 mL) va 1 (0.121 g/100 mL).

Gia tri P lien quan den kieni dinh F ciia md hinh (F-test hay Idem dinh Fisher) d Bang 6 cd gii tri P = 0,001 < 0,05 cho thay dd tirong thich ciia phuong trinh hdi quy (1) vdi thue nghiem tir dd cho thay dp tin ciy thdng ke. He sd xac dinh R- li 90,56% su bidn doi cua khdi lupng sinh khdi khd do anh hudng cic yen td khao sit va chi cd 9.44% su thay doi li do cac yeu td khdng xie dinh dupc gay ra. Ben canh dd, h§ sd bien thien CV = 13,01% chiing td rang thi nghidm dupc thuc hien chinh xac va co dp lap lai cao (Box et al. 1978). Miic dp phu hpp cua md hinh cdn dupc the hien qua gia tri P eiia Lack of fit (su khdng phii hpp). Theo Zabeti etal (2009). md hinh tuong quan tdt can cd su phii lipi) giira sd lieu thuc te vi ly thuyet, vi viy md hinh thu dupe Lack of fit khdng cd y nghia thdng ke la dieu mong mudn. Tir kdt qua phan tich b bang 8 cho tliay Lack of fit khdng cd y nghia tiidng kd (P > 0,05) cho Uiay su phii hpp cua md hinh la rat cao.

Nghidm thuc

1 2 3 4 5 6 7 S 9 10 11 12 13 14 15 16 17 IS 19 20

Ghi chu: Cac

Nhan td thu nghiem

pH

5.00 5,00 5,00 5,00 5,59 5,59 5,00 4,00 4,41 5,00 4,41 4.41 5,59 5,00 5,00 4,41 5,00 5,00

Mat sd (tb/mL)

10' 10' 10' 10' 10' 10' 10» 10' 10' 10' 10' 10' 10' 10= 10' 10= 10' 10'

Peptone (K/L) 0,300 0,000 0,300 0,300 0,122 0,122 0,300 0,300 0.122 0.300 0,122 0,478 0,478 0,300 0,300 0,478 0.300 0,600

5,59 1 10' ' 0,478 6,00 IO' 0,300

gia tri thu V nghiem trong bang la gia tri trung

Ham muc tieu ITiuc nghiem (g/100 mU

0,326 0,121 0,279 0,257 0,279 0,206 0,386 0.373 tX229 ' 0.334 0,102 0.414 0.347 0,382 0,304 0,410 0,329 0,420 0,469 0,317

binh cua 3 lin lip lai.

Du doan (g/100 mL)

0,319 0,100 0,319 0,319 0,273 0,205 0.319 0 332

'J,361 — _i!jK)^ - - - _ ! i 4 2 2 _ ^ . - - _ a 3 1 9

-:-- ]S5~

94 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - T 'Mr-'•' G 11/2019

KHOA HOC CONO NGHE

Bdng

Ngudn bidn thien

Md hinh

X,-pH X rmat sd X [-peptone

X,X, X,X, X.J(,

x,= x/ X,' Residual Lack of Fit Sai sd R

3. Kdt qua phan Tdng binh

phuong

0,160 1,597 5,459 0,130 3,763 6,128 0,013 14,019 10,039 12,255 0,0170 17,260 0,010 0,9056

tich ANOVA cua md hinh da thiic bac hai

Bac tu do

9

10 5 5

Tnmg bmh i „. . _ „ . . . . Gia tri F bmh phuong ]

0,018 10,660 1,597 5,459 0,130 3,763 6.128 0,013 14,019 10,039 12,255 4,510 3,430 5,580

R'-hieu chinh 0,8270

0,120 1,860

77,290 2,250 2,010 7,920 0,400 0,310 3,360

0,620

Gia tri P

0,001 0,733 0,202

<0,0001 0,164 0,187 0,018 0,543 0,590 0,097

0,696

Qua trinh sin xuit sinh khdi nim men thuc hien dua tren mdi quan he giira khdi lupng sinh khdi khd va cac yen td inh hudng trong mdi trudng nudi eay. Mdi quan hd niy dupx: thd hien qua phuong trinh da thlic bic hai sau:

Y = 0,32 + 7,555Xr 0,015X^ + 0,097Xrj + 0,022XiX, • 0,020X,X;, •*• 0,041X:PC3 + 15,435X,' +13.274X,r -0,020X;, (1)

Trong dd, Y li khdi lupng sinh khdi khd mong dpi; X,, X , X, lan lupt la gia tri pH, mil sd nam men va h^m lupng peptone.

Bang 9. Kdt qua thi nghidm

Trong vimg khao sit, phuong trinh hdi quy (1) cho thiy khdi luong sinh khdi khd chiu anh hirdng bie mdt va bic hai aia ydu td peptone (X3) vi chiu anh hudng cua cap yeu td mat sd va him lupng peptone (X2*X3). Dua vio phuong trinh hdi quy cd thd thiy ham lupng peptone cd anh hudng rat ldn ddi vdi khdi lupng sinh khdi khd thu dupc d ca bac mdt va bic hai. Dieu nay ed the giai thich ring ham lupng dam trong mdi trudng nude ehua tiu hu khdng dip ling day du cho nhu cau cua nim men.

xie nhan didu kidn tdi uu

Nghiem thuc

1

2

3

4

Yeu td

pH

4,41

4,41

5,00

5.59

Mat sd

(tb/nlL)

10'

10''

10«

10'

Peptone

(g/L)

0.478

0.478

0,600

0,478

Ham muc tieu

Thuc nghiem

(g/100 mL)

0,413

0,397

0,412

0,443

Dudoan

(g/100 mL)

0,429

0,422

0,426

0,440

Ghichu: Cacgia tn time nghiim tiong bang la gta Ket qua d bang 9 trinh biy 4 nghiem thuc dupc

md hinh CCD du doin cac thdng sd cd iuong sinh khdi cao nhit va dupc xac nhin bang ket qua thuc nghidm. Cac ket qua thu dupc hi thuc nghiem khdng cd sir chenh lech so vdi kdt qua du doan tir md hinh cho thay su phii hpp cua md hinh tdi uu hda. Trong do, nghiem tliirc 4 cho sinh khdi eao nhit (0.443

tii ti-ung binh ciia 3 lan lap lai. g/100 mL) vdi cie dieu kien duoc xae dmh gdm pH 5,59, mat sd gidng chiing 10 te bao/mL va ham lupng peptone bo sung li 0,478 g/L. Kdt qua tdi uu hda vdi viec bd sung peptone, dieu chinh pH va sti dung mat sd gidng chiing thich hop de sin xuat sinh khdi nim men tren moi truong nude ehua tiu hu da thu dupc ket qua kha quan vdi 0,443 g/100 mL So

NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG 11 /2019 95

KHOA HOC CdNG N G H l

vtn ket qui a bang 4, khi nudi nam men 5 cerevisiae LXl.l trong mdi tnrong nud«c chua tau hu ban diu, lirong sinh khdi chi dat 0,204 g/ KK) mL Lupng sinh khdi thu duoc trong mdi tniong nuoe chua tiu hu duoc tdi uu hda cung da dat tuong duong vdi mdi trudng YPD (0,443 g/100 mL so vdi 0.449 g/100 mL).

3.5. Anh hudng cua nhidt dp vi thdi gian d^n qui trinh tu phin ciia nim men

Sinh khdi nam men dupe nudi trong mdi trudng nude ehua lau hu dupe thu nhan va thuc hien qua trinh tu phin d cie miic nhiet dd vi thdi gian da bd tri dd thu hdi yeast extract, ket qui dupe trinh bay d bang 10. Kdt qua cho thi'y him lupng yeast extract thu duoc giira cac nghiem thiie thay ddi theo nhirng muc nhidt dd khac nhau. Him lupng yeast extract thu dupe cao nhat (he hien d nghiem thiic tu phan d 50 C trong 48 gio (0,298 g). tidp den la nghiem thiic tu phan d 55°C trong 48 gid (0.263 g), nghiem thiic tu phan d 55°C trong 72 gid (0,259 g) va giam dan d eic nghiem thiic cdn lai, thip nhit la b nghiem thiic tu phin b 45°C trong 24 gid (0.209 g) Cac nghiem thuc nay khae biet ed y nghia \(' mat thdng ke va cd su luong lac lin nhau voi gii tri P = 0.00 < 0,05. He sd R-= 0,95 eho thiy su bidn ddi cila khdi luong yeast extract thu dupe do inh hircmg ciia cic yeu td khao sal va chi cd 5% su thay ddi do cac yeu td ngau nhien. Bdn canh dd, he sd bien thien CV = 8,54% eho thay thi nghiem dupc thue hien chinh xac va cd dd tin ciy cao (Qoxetal. 1978).

Bing 10. Anh hirdng ciia nhi§t dO vi thdi gian dhi qui hinh ty phin thu yeast extract

Nhiet dd CO

45

50

55

C\'A,

Thdi gian (gid) 24

48

72

24

48

72

24

48

72

8,54

Trung binh (g/3,0g)

0,209'

().259''-

0.227-'

0.243"'

0.298'

0.2,59'

0.220''

0.263''

0.230'-

% Ghi chu: CJC gia tii trong bang la cac gia tri

trung binh ciia 3 lan lap lai. Cac gia tii trong cung mdf cot CO mdu tir gidng nhau tbi khac biet khdng cdy nghia thdng ke d muc 5% (P<0.05).

Kel qua eho thay qua trinh ta phiii cu.i nim men thu yeast extract cao nhal. dat ():l\^> -': 3,0 g sinh khdi khd td bao nim men, d nhiet do 50'C va thdi gian li 48 gid. Su khac biet ve ham luong yeast exti-act thu dupe li do nhiet dp va thdi gian hr phin khac nhau. Trong thdi gian ngin va nhiet dd thap (45X trong 24 gid), nim men tir phan chua triet de, cic mach polypeptide chua bi cit dut, cdn ndu ti-ong thdi gian qua dai v i ket hpp d nhidt dp cao (55°C trong 72 gid) se gay bien tinh lim tiiat thoat ham lupng yeast exti-act. So vdi nghien ciiu thiiy phin men bia bing phuong phip tu phan cua Tanguler va Erten (2007) vdi hidu suit thu yeast extract dgt So vdi nghien cim thuy phan nam men bia bing enzyme ciia In et al. (2005), hieu suat thu yeast extract dtit 50% khdi lupng nam men, hieu suit tu phin nam men tir nude chua tiu hu la thap hon. Tuy nhien, vide ling dung qua U'inh tuphin nam men cd the lim giam chi phi dau tu ngudn enzyme thuy phin, cd tiem nang ling dung tren quy md cdng nghiep.

3.6. Tliu hdi p-glucan trong nim men S. cerevisiae dupc nudi cay bing nude chua tiu hii

Bang 11. Anh hudng ctia ndng dp NaOH ddn vi^c tich chi^t p-glucan tong sd vi hi^u suat thu hdi p-

glucan

Nghiem thuc

1 2 3

4

CV(%)

Ndng dp NaOH (%w/v)

2,0

2,5 3,0

3,5

P-glucan tdng sd

(g/3,0g) 1,619'-

2,197''

_ 2 , 5 8 3 ; i

2,561'

10,48

Hieu suat thu hdi

(%) 53,96"

73,23'

86,10"

85,37-

9,84 Ghi chu: Cac gia tn' trong bang li cac gia tri

tmng bmh ciia 3 lin lap lai Cic gii tri trong cing •^ot cdt CO miu tugiSng nhau thi khic biet khdng cd y nghia thdngke a miic 5% (P<0,0.5)

Ket qua d bang 11 cho thaj: ham luong ()-glucan long so thu dupc giua cac nghiem thuc thay ddi d nong dp NaOH khac nhau. Ham l„™,g . J , duoc cao nhat d nghiem Ihuc 3 voir 3,0% (2,583 g, hieu suat 86,l(i'lf.)

jlucan thu ' I'-ng dp NaOH I do la p nghiem

-'^''1 g, hifiu suat

(2.197 g, hieu suat 73,3%) v a * : ; f „ ^ | ^ ^ " ^ ^ %

thuc 1, vdi ndng dd NaOH 2S. (1,619 "'^'"™

thuT 4 vdi ndng do NaOH 3,5

I tir 3,0 g sinh khdi kho t<- bao qua thu duoc thSp hon so v6i thi ngi* m?i, ™"- ^^

^ " | " - i n ChlP't (A^v. a

96 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - TH NS

hieu suat men. Kdt idt lach ^

11/2019

KHOA HOC CONG NGHE

glucan tir men bia bang sieu im ciia Tam et al. (2013), vdi hieu suat dat 72,06%.

4. KET LUAN

Tuyen chpn dupc chting S. cerevisiae LXl.l vdi kha cho sinh khdi tdt nhat, dat 0,204 g/100 mL mdi truong nuoe chua tiu hu. Khao sat cie yeu td anh hudng den kha nang tao sinh khdi ciia chung 5. cerevisiae LXl.l cho thay yeu td pH, mat sd va him lirpng yeast extract cd tac ddng y nghia thdng qua md hinh PBD. Ket qua thdng ke tir md hinh thuc nghipm CCD cho thay S. cerevisiae LXl.l phit trien tdi uu trong dieu kien mit sd 10' tb/mL, ham lupng peptone bo sung 4,78 g / L v i pH 5,59 vdi khdi lupng sinh khdi khd thu dupc la 0,469 g/100 mL. Kdt qua thii nghiem thu hdi yeast extract va p-glucan tir sinh khoi nam men S. cerevisiae nudi cay trong mdi trudng nude chua tiu hu vdi 0,298 g yeast extract va 2,583 g p-glucan txr 3,0 g sinh khdi khd te bio nam men vdi mux: thu hdi dat li 86,10%. Kdt qua cung cho thay tidm ning dug dung cua mdi trudng nude chua tiu hu li mdt mdi trudng re tien trong nudi cay nam men dd thu sinh khdi cung nhu chiet tach cac chat CO gia hi tir nam men.

i n CAM OIU

Xm chan thanh cam on su hd tig- kinh phi tu Trudng D^ hoc Can Tlia thdng qua di tai nghien cihl khoa hgc cap casd T2018-106.

TAI UEU THAM KHAO

1. AbuMweis, S. S., S. Jew, N.P. Ames., 2010. p-glucan from barley and its lipid-lowering capacity: A meta-analysis of randomized, controlled trials. European Journal of Clinical Nutrition, 64(12). 1472-1480.

2. Adoki, A, 2008. Factors affecting yeast growth and protein yield production from orange, plantain and banana wastes processing residues using Candida sp. Afiican Journal of Biotechnology, 7(3), 290-295.

3. Behall, M. K ,D . J. Scliolheld,J. Hallfrisch , 2004. Diets containing barley significantly reduce lipids m mildly hypercholesterolemic men and women. The American Joumal of Chnlcal Nutiition, 80(5), 1185-1193.

4. Box, G. E. P, W. G. Hunter, J. S. Hunter., 1978. Statistics for Expenmenters. NewYork: Wiley, 291-334.

5. Chae, H. J.. H. Joo, M. J. In., 2001. Utilization of brewer's yeast cells for the production of food-grade yeast extract. Part 1: effects of different enzymatic treatments on soUd and protem recovery and flavor characteristics. Bioresoures Technology. 76(3). 25^-258.

6. Di Serio, M.. P. Aramo, E. de Alteriis. R. Tesser, E. Santacesaria., 2003. Quanhtative Analysis of the Key Factors Affecting Yeast Growth. Industrial and Engineenng Chemistry Research, 42(21), 5109-5116.

7. Dirong Anh Nguyet, 2012. Khao sat diiu kien nudi cay di san xuat smh khdi nam men. Luin vSn tdt nghiep dai hoc. Trudng Dai hoc Cin Tlio. 66 trang.

8. El-Bakry, M., J. Abraham, A. Cerda, R. Barrena, S. Ponsa, T. Gea.. 2015. From wastes to high value-added products: Novel aspects of SSF in the production of enzymes. Journal Cntical Reviews in Environmental Science and Technology. 45(18), 1999-2042.

9. Ferreha, I. M. P. L V. O., O. Pmho, E. Vieira, J. G. Tavarela., 2010. Brewer's Saccharomycesyea^t biomass: characteristics and potential apphcations. Trends in Food Science & Technology, 21 (2), 77-84.

10. In, M. J., Kim, D. C, Chae, H. J., 2005. Downstream process for the producUon of yeast extract using brewer's yeast cells. Biotechnology and Bioprocess Engineering, 10, 85.

11. Jesenak, M., I. Urbaneikova, P. Banovcin., 2017. Resphatory tract mfeetions and the role of biologically active polysaccharides in their management and prevention, .\nincnis '}(7),779.

12. Umtong, S., C. Sringiew, W. Yongmamtchai., 2007. Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresource Technology, 98(17), 3367-3374.

13. Luong Diic Pham, 2009. Nam men cdng nghiip NXB KHKT, Hi Noi.

14. Munu-aj, I. K. S. K Utiiandi, Z. Hu, L Xiao, X. Zhan., 2015. Microbial lipid production from renewable and waste matenals for second-generation biodiesel feedstock. Environmental Technology Reviews, A{1), 1-1^.

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15. Ounis, W . C. P. Ben, J. Champagne, L. Bazinei., 2008. Ltilization of tofu whey pre-treated by electromembrane process as a growth medium for Lactobacillus plantamm LB17. Desalination, 229(1-3), 192-203.

16. Pena, A, N. S. Sanchez, H. Alvarez, M. Calahorra. J. Ramirez., 2015. Effects of high medium pH on growth, metabohsm and transport in Saccharomyces cerevisiae. FEMS Yeast Research, 15(2),fou005.

17. Pham Viet Cudng, Nguydn Thi Kim Cue, Pham Due Thuan, Hoing Thi Huong, 2005. Nghiin cuu xay dung cdng nghe san xudt beta-glucan tti thanh ti bao nim men dimg trong cdng nghiip thuc pham, dugc pham va my pham. Bio cao de tii NCKH Chuong tiinh KC.04.28. 89 frang.

18. Podpora, B., F. Swiderski, A Sadowska, A. Piofrowska, R. Rakowska., 2015. Spent brewer's yeast autolysates as a new and valuable component of functional food and dietary supplements. Joumal of Food Processing and Technology, 6, 526-530.

19. Podpora, B., F. Swiderski, A Sadowska, R. Rakowska, G. Wasiak-Zys., 2016. Spent brewer's yeast extracts as a new component of functional food. Czech Joumal of Food Sciences, 34(6), 554-563.

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21. Sanjay, K. R-, R. Subramanian, A Senthil, G. Vijayalakhmi.. 2008. Use of natural coagulants of plant origin in production of soycurd (Tofu). International Joumal of Food Engineering, 4(8), 45-50.

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24. Tam, T. .M. N. Q. Duy, N. P. Minh, D. T. A Dao., 2013. Optimization of peta-glucan cxu^ction

from waste brewer^s yeast Saccharomyces t erevislae using autolysis, enzyme, ultrasonic and combined enz\'me - ulfrasomc treatinent. American Joumal of Research Communication, 1(11), 149-158.

25. Sintawarrdani, N., 2011. Socio-Econimic Problems on Reducmg the Waste water Pollution from Tofu Processing in the Cibuntu Area, Indonesia. Research Center for Physics Indonesian Institute of Science, Indonesia.

26. Takara K, Ushijima K., Wada K, Iwasaki H., Yamashita M., 2007. Phenolic compounds from sugarcane molasses possessing antibacterial activity against canogenic bacteria. J Oleo Sci., 56(ll):611-4.

27. Thi, L N.,C. P. Champagne, B. H.Lee, J. (ioulet, 2003. Growth of Lactobacillus paracaselssp. paracasei on tofu whey. International Journal of Food Microbiology, 89(1), 67-75.

28. Tran Vu Phuong (2011). Khao sat cac yeu id anh hirdng den qua trinh tang smh khdi cua Bacillus subtilis tiong mdi tnrdng nude thai tau bu. Luan van Thac si nginh Cdng nghe Sinh hpc, Vidn NC & PT Cong nghe Sinh hpc, Trudng Dai hpc Can Tho.

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32. Vieira, F. E., J. Camlho, E. Pmto, S. Cunha, A A Almeida, I. M, P. L. V. 0. Fen-eira., 2016. Nutntive value, antioxidant activity and phenolic compounds prohle of brewer's spent yeast exfract Joumal of Pood Composition and Analysis. 52, 44-

33. Zabeti, M.. W. M. A W. D.^d. M K. Aroua. 2009. Optimization of tiie acUvit>- of ' c ^ O M m ' catalyst for biodiesel production nsing ™ e surface methodology. Apphed Caialvsi<rA- r i 366(1), 154-159. " ^ ^'^"^''^'

98 NONG NGHIEP VA PHAT TRIEN NONG THON - KY 2 - THANG l

KHOA HOC CONG NGHE

STUDY ON THE RECOVERY OF BIOMASS, YEAST EXTRACT AND p-GLUCAN FROM

Saccharomyces cerevisiae CULTIVATED ON TOFU SOUR UQUID MEDIUM

Nguyen Ngoc Thanh^ Dang Thanh Nhan', Tran Thi Hong Van', Bui Hoang Dang Long', Huynh Xuan Phong^

'Biotechnology Research and Development Institute, Can Tho University

Summary The aims of the study were to select the strain of Saccbaromyces cerevisiae and optimize the conditions for biomass production in tofu sour liquid medium, and to invesUgate the conditions for the acquisition of total yeast extract and p-glucan from yeast biomass. 5. cerevisiae LXl.l was selected with high biomass production (0.204 g/100 mL) in tofu sour liquid at pH 5.53 from 10 tested yeast strams. Ey experimental Plackett-Burman design (PBD), 7 factors affecting the yeast autolysis were investigated. Tliree factors including pH, initial cell level and peptone concentraUon which statistically significant affected on yeast biomass were continued lo optimize using CenUal Composite design (CCD), The results showed that the suitable growth conditions for 5 ce/'ews/ae LXl.l were determined at 10' cells/mL, 4.78 g/L supplemented peptone and pH 5.59. the dried biomass was obtained at 0.469 g/ lOOmL In total. 0.298 g of yeast extract and 2.583 g of p-glucan were achieved from 3.0 g of dried yeast cells and the recovery ratio of p-glucan was obtained at 86.12%.

Keywords: p-glucan, Saccbaromyces cerevisiae, toAi sour liquid, yeast extract Ngudi phin bi^n; TS. Nguydn Manh DOng Ngiy nhin bii: 28/6/2019 Ngiy thdng qua phin bi^n: 29/7/2019 Ngiy duy^t ding: 5/8/2019

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