Discover Bio Manual Peptide Synthesizer - CEM Corporation

Discover Bio

Manual Peptide SynthesizerInstallation and Operation Instructions

Contents

600423 Rev. 2

August 2017

Introduction to the Discover Bio 2

System Requirements 3

Hardware Assembly and Setup 4

Wash Station Setup 4

Discover Setup 7

General Instrument Operation 8

Programming a New Method 8

Loading an Existing Method 8

Recommended Synthesis Parameters 9

Recommended Microwave Methods 9

Recommended Reagents for 0.1 mmol Scale 9

General Discover Bio Methods 11

Swelling the Resin 11

Performing a Deprotection 12

Performing a Coupling 12

Performing a Cleavage 13

Using the 4 mL Vessel for Smaller Scale 15

Connecting the 4 mL Vessel 15

Maintenance Procedures 16

After Each Use 16

Monthly Maintenance 16

Semiannual Maintenance 16

Replacement of RV Frit 16

Spare Parts and Accessories 18

System 18

Accessories and Consumables 18

Discover Bio™ Manual Microwave Peptide Synthesizer600423 • Revision 2 • August 2017

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Introduction to the Discover BioThe Discover Bio Manual Microwave Peptide Synthesizer is the newest manual microwave-enhanced peptide synthesis system. Built on CEM’s flexible Discover microwave platform, the Discover Bio allows the rapid and efficient synthesis of peptides on scales from 0.05 to 0.5 mmol thanks to the efficient washing station and CEM’s patented circular microwave cavity.

Product Position

Nitrogen Valve

Left Knob

Add Port

Right Knob

Power Switch

Discover Bio Microwave Reactor

Discover Bio Wash Station

Reagent Bottles

Discover Bio™ Manual Microwave Peptide Synthesizer600423 • Revision 2 • August 2017 3

System Requirements

Bench and/or Fume Hood SpaceThe Discover Bio should be positioned on the bench such that access to the electrical outlets for the system is not restricted. The Discover Bio requires the following space for system components:

Discover Bio Microwave Reactor

15” (w) x 20” (d) x 9” (h) [38 cm (w) x 51 cm (d) x 23 cm (h)] (Depth includes 3” (8 cm) clearance behind instrument for unimpeded airflow at rear fan ducts)

Discover Bio Wash Station

11” (w) x 11” (d) x 13” (h) [28 cm (w) x 28 cm (d) x 33 cm (h)]

Bottle Holder with Bottles

9.5” (w) x 9” (d) x 11” (h) [24 cm (w) x 22 cm (d) x 28 cm (h)]

Environmental Conditions

The Discover Bio is designed for indoor used only.

Temperature Range: 50 °F – 85 °F (10 °C – 29 °C) Relative Humidity Range: 0 – 85%

Inert Gas Source

The Discover Bio requires an inert gas source (either high purity grade nitrogen or argon) capable of supplying 20 psi (20 L/min flow) within ten feet (10’)/three meters (3 m) of the rear of the instrument.

Electrical Requirements

The Discover Bio requires electrical power of 120 VAC (60 Hz, 1.7 A) (or 240 VAC [50 Hz, 1.7 A] where applicable). Specific power requirements (120 VAC vs. 240 VAC) can be found on the nameplate affixed to the rear of the Discover Bio Microwave Reactor and on the rear of the Discover Bio Wash Station.

Safety InformationFume Ventilation

The Discover Bio operates as a semi-closed system, with minimal venting of any hazardous solvent fumes through the vent line. The vent line must be vented into a proper chemical fume hood or exhaust line no longer than 10 feet (10’)/three meters (3 m) from the instrument.

In addition, adequate ventilation should be provided for preparation of reagents and solvents for use on the system. All solvent bottles and the Waste Reservoir should be placed into proper secondary containers to minimize the risk of exposure.

Waste Disposal

Waste produced by the Discover Bio can be hazardous. For detailed information on the safety requirements for the chemicals used on the Discover Bio, refer to the appropriate SDS documents.

WARNINGHandle all waste under a fume hood, and wear suitable protective clothing. Dispose of all waste in accordance with all applicable local, state, and federal heath and safety recommendations.


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Hardware Assembly and Setup

Wash Station Setup

When setting up the wash station, all tubing connections will be made on the rear of the unit. There are eleven ports on the manifold, as well as two stainless steel ports. The proper connections for the manifold are summarized in the table below.

Bottle Setup

1. Place the three 1 L glass bottles in the bottle holder.

2. Set up the Wash Solvent bottle.

2.1. Connect a bottle cap to the 1 L bottle to be used for wash solvent. Ensure the dip tube reaches the bottom of the bottle and that the dip tube filter is in place.

2.2. Connect the bottle tubing to the cap. The tube labeled “LIQ” should be connected to the same port as the dip tube. The unlabeled tube should be connected to the port without a dip tube.

2.3. Connect the other end of the bottle tubing to the rear of the Wash Station. The tube labeled “LIQ” should be connected to position 4. The unlabeled tube should be connected to position 1.

3. Set up the Deprotection bottle.

3.1. Connect a bottle cap to the 1 L bottle to be used for deprotection solution. Ensure the dip tube reaches the bottom of the bottle and that the dip tube filter is in place.



Position Number Tubing Connection

1 Main Wash Nitrogen

2 Deprotection Nitrogen

3 Waste Vacuum

4 Main Wash Liquid

5 Deprotection Liquid

6 Waste Liquid

7 Reaction Vessel Sprayhead

8 Reaction Vessel Drain

9 Reaction Vessel Vent

10 Product Position Vacuum

11 Product Position Liquid

SS-1 Nitrogen Source

SS-2 System Vent


4. Set up the Waste bottle.

4.1. Connect a bottle cap to the 1 L bottle to be used for waste. Cut the dip tube to approximately 1.5” (~4 cm) in length.



Nitrogen and Vent Line Setup1. Connect the nitrogen source to stainless steel port 1. Set the incoming pressure to approximately

20 psi.

2. Cut a piece of tubing (no greater than 10’ (~3 m) in length) and connect it to stainless steel port 2 using a stainless nut. This will serve as the vent line for the entire system. This tubing should be run into a ventilated fume hood.

Product Collection Position Setup1. Cut two pieces of tubing approximately 18” (45 cm).

2. Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the left port on the rear of the Product Collection Position, then connect the other end to position 10.

3. Take one piece of tubing and, using yellow ferrules and nuts, connect one end to the right port on the rear of the Product Collection Position, then connect the other end to position 11.

Standard (25 mL) Vessel Setup1. Open the reaction vessel by pressing the vessel body up into the vessel top and rotating.

2. Connect the sprayhead line.

2.1. Unscrew the sprayhead. Cut a length of tubing (~48” (120 cm)). Thread one end through the center port on the reaction vessel top.

2.2. Put a yellow ferrule onto the end of the tubing so that the smaller end is near the end of the tube, then press the end of the tubing into the sprayhead. Screw the sprayhead back into the sprayhead port to secure the line.

2.3. Using a yellow ferrule and nut, connect the other end of the tubing to position 7 on the rear of the Wash Station.


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2.4. Reconnect the reaction vessel body to the top by pressing the body up into the top and rotating.

3. Connect the thermowell.

3.1. Place a yellow ferrule and nut on the end of the thermowell.

3.2. Insert the thermowell into one of the side ports of the reaction vessel top so that the bottom of the thermowell is just resting on the bottom of the vessel. Secure the thermowell with the nut.

4. Connect the reaction vessel vent line.

4.1. Cut a length of tubing (~48” (120 cm)). Using a yellow ferrule and nut, connect the tubing to the other side port of the reaction vessel top, being careful to bend the sprayhead line out of the way.

4.2. Using a yellow ferrule and nut, connect the other end of the tubing to position 9 on the rear of the Wash Station

5. Connect the RV Drain line.

CAUTIONDo not overtighten the fitting on the thermowell, as this can break the thermowell and damage the fiber-optic probe.


5.1. Cut a length of tubing (~60” (150 cm)). Using yellow ferrules and nuts, connect one end of the tubing to the port on the bottom of the vessel.

5.2. Connect the other end to position 8 on the rear of the Wash Station.

Discover Setup

Temperature Probe Assembly1. Connect one end of the serial cable to the rear of the FISO temperature control box. Connect the

other end of the serial cable to the COM2 port on the rear of the Discover.

2. Connect the blue fiber-optic temperature probe to the FISO control box, taking care not to bend the probe.

Calibration of the Fiber-Optic Probe

1. Connect the power cord to the rear of the Discover. Plug the power cord into an appropriate power source. Locate the power switch on the left side of the Discover and turn the power on.

2. After the Discover has started up, press the EDIT key to open the System Menus.

3. The first menu option will be Temperature. Press ENTER to open the Temperature Menu.

4. Use the right arrow key to scroll to “Select Alternate” and press ENTER.

5. Use the right arrow key to change to “DEVICE = FIBER-OPTIC” and press ENTER.

6. The Discover will return to the Home screen. Press EDIT to open the System Menus, then press ENTER to open the Temperature Menu again.

7. Use the right arrow key to scroll to “Enter Calibration” and press ENTER.

8. Press EDIT, then use the numeric keypad on the right of the Discover to enter the seven digit GF number printed on the white band at the base of the blue fiber-optic probe.

9. Ensure the GF number is correctly entered, and press ENTER to save the calibration value in the software.

10. Press HOME to return to the Home screen.


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Setting Discover for Open Vessel Mode1. From the Home screen, press EDIT to open the System Menus.

2. Use the arrow keys ( and ) to scroll to Open Vessel and press ENTER.

3. Use the right arrow key to set “RUN OPEN VESSEL: YES” and press ENTER.

4. The Discover will return to the Home screen. Ensure that the top line of the screen says “OPEN VESSEL”.

5. Insert the Open Vessel Attenuator so that the two tabs line up with the two slots, and lock into place.

General Instrument Operation

Programming a New Method

1. Press the OPEN key.

2. Use the left arrow key to select “New Method” and press ENTER.

3. The first parameter will be Mode. Use the right arrow key to select SPS mode and press ENTER.

4. Using the numeric keypad, enter the microwave power setting (in W) and press ENTER.

5. Using the numeric keypad, enter the maximum temperature setting (in °C) and press ENTER.

6. Using the numeric keypad, enter the run time (in min:sec) and press ENTER.

7. Using the numeric keypad, set the Delta Temperature value to 5 °C and press ENTER.

8. Using the right arrow key set stirring to “OFF” and press ENTER.

9. Using the right arrow key set cooling to “OFF” and press ENTER.

10. Using the right arrow key set “NEXT STAGE = (N)” and press ENTER.

11. Using the right arrow key set “SAVE METHOD = (Y)” and press ENTER.

12. Using the arrow keys ( and ) to scroll to the desired characters and ENTER to select, enter a name for the method. When the name has been entered, use the arrow keys ( and ) to highlight “Exit” and press ENTER. The method is now saved in the firmware.

Loading an Existing Method

1. Press the OPEN key.

2. Use the arrow keys ( and ) to scroll to the desired method name, then press ENTER to load the method.

3. The method name should be displayed on the Home screen, along with the programmed parameters.


Recommended Synthesis Parameters

Recommended Microwave Methods

Recommended Reagents for 0.1 mmol Scale

Activator Base SolutionPrepare a solution of ~0.3 M DIEA in DMF.

Deprotection SolutionPrepare a solution of 20% piperidine in DMF with 0.1 M HOBt monohydrate.

Method Power Max Temp. Run Time Delta Temp.

Deprotection 20 W 75 °C 3:00 5 °C

Coupling 20 W 75 °C 5:00 5 °C

TFA Cleavage 15 W 38°C 30:00 5 °C

NOTECysteine and histidine couplings should be performed at 50 °C to reduce racemization. Cleavage temperatures should never exceed 43 °C. All values for Power are suggested, and should be adjusted depending on reaction scale. The goal is to reach the maximum temperature in 75 seconds for Deprotection and Coupling, and in 120 seconds for Cleavage.

Number of Couplings

0.1 mmol Scale

mL DMF

mL DIEA

Final Vol.

5 19 1 20

10 38 2 40

Per Coupling: 4 mL

Final Volume

0.1 mmol Scale

mL DMF

mL Pip.

g HOBt monohydrate

50 40 10 0.77

100 80 20 1.5

500 400 100 7.7

Per Coupling: 7 mL

NOTEHOBt is optional, but is recommended for sequences susceptible to aspartimide formation.

WARNINGThe Discover Bio reaction vessels should not be operated above 75 ºC.


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Amino Acids and ActivatorBefore beginning a synthesis, weigh out the required amino acid and activator for each coupling into a separate vial.

Amino Acid MW

0.1 mmol Scale

g AA g HBTU

Fmoc-Ala-OH 311.3 0.156 0.190

Fmoc-Arg(Pbf)-OH 648.8 0.324 0.190

Fmoc-Asn(Trt)-OH 354.4 0.298 0.190

Fmoc-Asp(OtBu)-OH 411.5 0.206 0.190

Fmoc-Cyc(Trt)-OH 585.7 0.293 0.190

Fmoc-Gln(Trt)-OH 610.7 0.305 0.190

Fmoc-Glu(OtBu)-OH 425.5 0.213 0.190

Fmoc-Gly-OH 297.3 0.149 0.190

Fmoc-His(Trt)-OH 619.7 0.310 0.190

Fmoc-Ile-OH 353.4 0.177 0.190

Fmoc-Leu-OH 353.4 0.177 0.190

Fmoc-Lys(Boc)-OH 468.5 0.234 0.190

Fmoc-Met-OH 371.5 0.186 0.190

Fmoc-Phe-OH 387.4 0.194 0.190

Fmoc-Pro-OH 337.4 0.169 0.190

Fmoc-Ser(tBu)-OH 383.4 0.192 0.190

Fmoc-Thr(tBu)-OH 397.5 0.199 0.190

Fmoc-Trp(Boc)-OH 526.6 0.263 0.190

Fmoc-Tyr(tBu)-OH 459.6 0.230 0.190

Fmoc-Val-OH 339.4 0.170 0.190


General Discover Bio Methods

Swelling the Resin

1. Set both knobs to “Stop”.

2. Turn on the nitrogen source, then turn on the nitrogen to the system by setting the nitrogen control knob to pressure (the knob should be pointing down) and the vacuum pump.

3. Slide the drain line out of the groove in the top of the vessel assembly. Open the reaction vessel by pressing the vessel body up and rotating.

Add the resin to the vessel. Seal the vessel by pressing the vessel body up into the vessel top and rotating. Slide the drain line back into the groove in the top of the vessel assembly. Place the vessel in the reaction vessel holder.

4. Set the left knob to “Wash Solvent” to begin adding solvent to the reaction vessel.

NOTEIt does not matter which “Stop” position is used on the right knob.


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5. When the desired volume of solvent has been added to the vessel, set the left knob to “N2 Purge (Wash)” to purge any solvent remaining in the lines. When all solvent has been purged into the vessel, set the left knob to “Stop”.

6. Allow the resin to swell for approximately 15 minutes. Optionally, the resin can be bubbled during swelling by setting the right knob to “N2 Bubbling” after the solvent has been added.

7. After the resin has swollen, drain the solvent from the reaction vessel by setting the left knob to “N2 Purge (Wash)” the right knob to “Waste”. Once all the solvent has drained from the vessel, set the right knob to “Stop”.

Performing a Deprotection

1. Set the left knob to “Deprotect” to begin adding deprotection solution to the reaction vessel.

2. When the desired volume of deprotection solution has been added to the vessel, set the left knob to “N2 Purge (Deprotect)” to purge any remaining deprotection solution in the lines. When all reagent has been purged into the vessel, set the left knob to “Stop”.

3. Set the right knob to “N2 Bubbling”.

4. Ensure the fiber-optic probe is fully inserted into the thermowell.

5. Insert the reaction vessel into the Discover cavity. Load the Deprotection microwave method, and press the Play/Pause key to begin the method.

6. When the microwave method is finished, remove the reaction vessel from the Discover cavity and place it in the vessel holder. Set the left knob to “N2 Purge (Wash)” the right knob to “Waste”.

7. When all the solvent has drained, set the right knob to “Stop”. Set the left knob to “Wash Solvent” and add approximately 7 mL of solvent to the vessel, then set the left knob to “Stop”. Optionally, to bubble the resin for a more efficient wash, turn the right knob to “N2 Bubbling” and allow the resin to bubble for five seconds.

8. Set the left knob to “N2 Purge (Wash)”, then set the right knob to “Waste” to drain the reaction vessel. Set both knobs to “Stop”.

9. Repeat steps 7 and 8 two more times.

Performing a Coupling

1. Set the left knob to “Add” and the right knob to “Stop”.

2. Dissolve the appropriate amino acid, activator, and activator base in a total volume of approximately 4 mL.

NOTEPurging the line will add an additional 1-2 mL of solvent to the reaction vessel.

NOTEPurging the line will add an additional 1-2 mL of deprotection solution to the reaction vessel.



3. Draw the coupling solution into a 10 mL syringe. Insert the luer tip of the syringe into the injection port. Inject the coupling solution through the injection port into the reaction vessel.

4. To purge the injection port line, draw air into the syringe, then inject the air through the injection port to fully purge the coupling solution into the reaction vessel.

5. Repeat step 4.

6. Set the left knob to “Stop”. Set the right knob to “N2 Bubbling”.

7. Ensure the fiber-optic probe is fully inserted into the thermowell.

8. Insert the reaction vessel into the Discover cavity. Load the Coupling microwave method, and press the Play/Pause key to begin the method.

9. When the microwave method is finished, remove the reaction vessel from the Discover cavity and place it in the vessel holder. Set the right knob to “Waste” to drain the reaction vessel.

10. When all the solvent has drained, set the right knob to “Stop”. Set the left knob to “Wash Solvent” and add approximately 7 mL of solvent to the vessel, then set the left knob to “Stop”. Optionally, to bubble the resin for a more efficient wash, turn the right knob to “N2 Bubbling” and allow the resin to bubble for five seconds.



Performing a Cleavage

1. Set both knobs to “Stop.” Turn off the nitrogen source.

2. Remove the solvent bottle, then connect a solvent bottle of DCM.

3. Turn on the nitrogen source.

4. Set the left knob to “Wash Solvent” and add approximately 7 mL of solvent to the vessel, then set the left knob to “Stop”. Optionally, to bubble the resin for a more efficient wash, turn the right knob to “N2 Bubbling” and allow the resin to bubble for five seconds.



NOTEPurging the line will add an additional 1-2 mL of coupling solution to the reaction vessel.



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7. Slide the drain line out of the groove in the top of the vessel assembly. Open the reaction vessel by pressing the vessel body up and rotating it counterclockwise.

8. Add the cleavage cocktail to the vessel. Seal the vessel by pressing the vessel body up into the vessel top and rotating. Slide the drain line back into the groove in the top of the vessel assembly. Place the vessel in the Discover.

9. Load the Cleavage microwave method, and press the Play/Pause key to begin the method.

10. When the microwave is finished, remove the reaction vessel from the Discover cavity and place it in the vessel holder.

11. Connect a clean, labeled centrifuge tube to the product manifold.

12. Turn the right knob to “Product” to drain the cleaved peptide into the centrifuge tube.

13. Remove the centrifuge tube containing the cleaved peptide. The peptide is now ready to be precipitated and analyzed.

14. Set the right knob to “Stop”. Set the left knob to “Wash Solvent” and add approximately 7 mL of DCM to the vessel. Set the left know to “N2 Purge (Wash)”, then set the right knob to “Waste” to drain the reaction vessel. Set both knobs to “Stop”.

15. Repeat step 12 two more times.

16. Open the reaction vessel and remove the spent resin for disposal.

17. Repeat step 12.

18. Set both knobs to “Stop.” Turn off the nitrogen source.

19. Remove the solvent bottle, then connect a solvent bottle of wash solvent.

20. Turn on the nitrogen source.


Using the 4 mL Vessel for Smaller ScaleThe 4 mL reaction vessel is used for small scale synthesis (below 0.1 mmol scale). The 4 mL vessel does not have a sprayhead. All additions and washes must be added manually by opening the top of the reaction vessel.

Connecting the 4 mL Vessel

1. Place a yellow ferrule and nut on the end of the thermowell. Insert the thermowell into the center port of the reaction vessel top so that the bottom of the thermowell is on the bottom of the vessel. Secure the thermowell with the nut.

2. Connect the RV Vent line to the side port of the reaction vessel top.

3. Connect the RV Drain line to the port on the bottom of the vessel.


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Maintenance Procedures

After Each Use

• Clean the thermowell by wiping with a dry, lint-free cloth.

• If cleavagae was performed, clean the Product lines.

• Connect a 50 mL centrifuge tube to the product position.

• Open the reaction vessel and add ~20 mL dichloromethane to the reaction vessel. Close the reaction vessel.

• With the pump turned on, turn the right knob to “Product” to drain the dichloromethane into the centrifuge tube.

• Repeat with an additional 20 mL dichloromethane.

Monthly Maintenance

• Replace the dip tube filters in the main solvent and deprotection bottles.

Semiannual Maintenance

• Replace the Wash Solvent with methanol and perform a wash of the reaction vessel.

• Replace the frit on the bottom of the reaction vessel.

Replacement of Reaction Vessel Frit

1. Remove the drain line from the bottom of the reaction vessel.

2. Open the reaction vessel by pressing up into the reaction vessel top and rotating.


3. Unscrew the bottom of the reaction vessel.

4. Using the frit removal tool, pop the frit out of the vessel body.

5. Insert a new frit into the vessel body.

6. Screw the vessel body into the bottom of the reaction vessel. Close the reaction vessel by pressing up into the reaction vessel top and turning counterclockwise. Connect the drain line to the bottom of the reaction vessel.


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Spare Parts and Accessories

System

Accessories and Consumables

Product Part Number

Discover Bio System 925496

Includes the following:

Discover Bio Microwave Reactor (select one)

120 v (60 Hz) Power Supply 909450


Power Cord (varies by country)

Discover Bio Wash Station (select one)



Power Cord (varies by country)

Fiber-Optic Temperature Control 541176

Discover Bio Accessory Kit 908410

Product Part Number

4 mL Reaction Vessel Assembly 543505

Replacement Frits for 4 mL Vessel (Qty 50) 215005

25 mL Reaction Vessel Assembly 543500

Replacement Frits for 25 mL Vessel (Qty 50) 215055

50 mL Polypropylene Centrifuge Tubes (Qty 25) 167150-M

1 L Glass Bottle 167550

Two-Port GL45 Bottle Cap with Dip Tube 167559

Tubing for Bottle Cap 216780-M

Dip Tube Filters (Qty 50) 167485-M

Bottle Holder 543510

Fiber-Optic Temperature Probe 314325

Replacement Thermowell 181110


Limited WarrantyWhat Is Covered:CEM Corporation warrants that the instrument will be free of any defect in parts or workmanship and will, at its option, replace or repair any defective part (excluding consumables) or instrument.

For How Long:This warranty remains in effect for 365 days from date of delivery to the original purchaser.

What Is Not Covered:This warranty does not cover parts or workmanship damaged due to:• Neglect, abuse or misuse, or theft• Damage caused by or to test samples,• Damage incurred during instrument relocation,• Damage caused by or to any attached equipment,• Use of incorrect line voltages or fuses,• Fire, flood, “acts of God” or other contingencies beyond the control of CEM Corporation,• Improper or unauthorized repair, or• Any other damage caused by purchaser or its agents.

Responsibilities of Purchaser:• To ensure warranty coverage, the purchaser must:• Use the instrument according to directions,• Connect the instrument properly to a power supply of proper voltage,• Replace blown fuses,• Replace consumables and• Clean the instrument as required.

How to Get Service:Purchaser should contact the Service Department of CEM Corporation or the nearest CEM subsidiary or dis-tributor for return authorization and for proper crating and shipping instructions to return instrument, freight prepaid, for service. On-site repairs by an authorized service technician are available through the CEM Service Department. Travel costs will be charged to the purchaser for onsite repairs.

Within the U.S. Outside the U.S.CEM Corporation CEM Corporation3100 Smith Farm Rd. 3100 Smith Farm Rd.Matthews, NC 28104-5044 Matthews, NC 28104-5044(800) 726-5551 (704) 821-7015Fax: (704) 821-4368 Fax: (704) 821-4368

Warranty Disclaimer:CEM Corporation hereby excludes and disclaims any warranty of merchantability or fitness for any particular purpose. No warranty, express or implied, extends beyond the face hereof. CEM Corporation shall not be li-able for loss of use of instrument or other incidental or consequential costs, expenses or damages incurred by the purchaser or any other user. This warranty is not transferable.

Purchaser’s Rights under State Law:This warranty gives the purchaser specific legal rights, and the purchaser may also have other rights that vary from state to state.

CEM CorporationPO Box 200

Matthews, NC 28106Tel: 800-726-3331Tel: 704-821-7015Fax: 704-821-7894

Email: [email protected]

FranceCEM μWave S.A.S.Immeuble Ariane

Domaine Technologique de Saclay4, rue Rene’ Razel

91892 ORSAY CedexTel: 33 (01) 69 35 57 80Fax: 33 (01) 60 19 64 91 Email: [email protected]

www.cemfrance.fr

Germany, Austria, Swit-zerlandCEM GmbH

Carl-Friedrich-Gauss-Str.947475 Kamp-LintfortTel: (49) 2842-9644-0

Fax: (49) 2842-9644-11Email: [email protected]

www.cem.de

IrelandCEM Technology (Ireland) Ltd.

Sky Business Centre9a Plato Business Park

DamastownDublin 15

Tel: +353 (0) 1 885 1752Fax: +353 (0) 1 885 1601


ItalyCEM S.R.L.

Via Dell’ Artigianato, 6/824055 Cologno al Serio (Bg)

Tel: (39) 35-896224Fax: (39) 35-891661


Japan

CEM Japan K.K.2-18-10 TakanawaMinato-ku, Tokyo

108-0074Tel: +81-3-5793-8542Fax: +81-3-5793-8543


United KingdomCEM Microwave Technology Ltd.

2 Middle SladeBuckingham Industrial Estate

Buckingham MK181WATel: (44) 1280-822873Fax: (44) 1280-822342

Email: [email protected] Bio™ Manual Microwave Peptide Synthesizer

600423 • Revision 1 • August 2017

CEM has been an ISO-certified facility since 1994.

Discover Bio Manual Peptide Synthesizer - CEM Corporation

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Transcript of Discover Bio Manual Peptide Synthesizer - CEM Corporation