BARBER-COLMAN GAS CHROMATOGRAPH

MoptL 5300

S{etoidPna^yZeC

MO,D 5340, hermatCo^aUCtN^ty

Which specialized

BARBER-COLMAN GAS CHROMATOGRAPHoffers you the greatest value for routine analyses?

MODEL 5320 Flame lonizationChromatograph A simple, in-expensive instrument for petro-leum, chemicals, drugs, liquors,foods, paint, and cosmetic fields.

• High Sensitivity — Low Noise

• Coiled Columns

• Integral Air Supply

• Manual TemperatureProgramming

• Dual Injection Ports

• Dynamic Range to 100 million

• Attenuation Ratio 300,000 to 1

PRICE: $1,190

MODEL 5300 Steroid AnalyzerFor clinical analyses of 17-ketosteroids. Model 5300 can alsobe used for estrogens and pro-gesterone metabolites with nochange in operating parameters.

• On-Column Injection

• All-Glass System

• Argon or Flame lonizationDetectors

• Easy to Operate

• Compact, Simple, Stable

• SCR Column TemperatureControl

• Pre-set Parameters

• High Sensitivity

• "Cook Book" Procedures

PRICE: $1,595

MODEL 5340 Thermal Conduc»tivity Chromatograph An inex•pensive unit, Model 5340 is ex--cellent for quality control forteaching purposes.

• Simple to Operafa

• High Sensitivity

• W-2 4-filament DetectorSystem

• Compact and Stabie

• Eight-step Attenuator

• Solid-State Components

• Manual TemperatureProgramming

PRICE: $1,080

For further data on these and other specialized units for pesticides and meta/ chelates, pleasecontact one of almost 50 Sales and Service Branch Offices — see the Yellow Pages.

BARBER-COLMAN COMPANYDept. M, 15157 Rock Street, Rockford, Illinois, U.S.A.

BARBER-COLMAN of CANADA, Ltd., Dept. M, Toronto & Montreal • Overseas Sales Mgrs.: Ad. Auriema, Inc., N.Y.

Circie number 2.

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PolyScience CorporationINTER.NATIONAL DIVISION

HAAKE ROTOVISCOfor laboratories concerned withbasic rheological investigationsand for plants where the measurement and control of viscosity isimportant. A variety of accessories are available to extend therange of applications.

ORIGINAL

PYRO .. MICROPYROMETER

Total Radiation Pyrometers

Optical and Colorimetric Pyrometers

Surface contact - immersion Pyrometers

HAAKE

ULTRATHERMOSTATS

are available in a variety of

models to meet every laboratory

requirement where exact tempera

ture control is required.

IKA.MICRO·UNIVERSAL AUTOMATfor carbon, hydrogen, nitrogen, oxygen, sulfur and

halogen determinations.

High Performance Calorimeters, Shaker-Stirrers

IKA·COMBITHERM

GRONERT -Infrared Solid Liquid Tester; KERN - Analytical and Precision Balances; DR. STEEG & REUTER - Polarisators, Crystal Optics; NEUBERGER - Drying, Heating Cabinets, Incubators; DR. SCHNEIDER - Optical Gauges,Optical Test Instruments; KUEHN - Special Microscopes for Industry and Lab. MACHEREY & NAGEL -Indicator, Reagents and Filter Papers; ENDRESS & HAUSER - Isotope-Capacitance-UItr as 0 nie, Level Switch Installations;CARL ZEISS, JENA - Electrophoresis Instruments, RefraC+om eters, Interferometers and Microscopes.

For information about these instruments please write us.

Watch for our exhibit at the Pittsburgh Conference.Penn-Sheraton Hotel, Club Floor, Booth C 49

PolyScience Corp. 909 PITNER AVENUE EVANSTON, ILLINOIS 60202 PHONE: 475.4399

Cirele No.5 for Haa~e, No. 25 for IKA, No. 26 for Zeiss. No. 27 for PYRO, No. 28 for Groneri, No. 29 for Kern, No. 30 for Dr. Steeg &Reuter, No.3 I for Neuberger, No. 32 for Dr. Schneider, No. 33 for Kuehn, No. 34 for Macherey & Nagel and No. 35 for Endress & Hauser.

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THE NEWGAS,CHROM

SUPPORT

Q stands for GAS-CHROM Q, the new support for you - particularly ifyou are using gas chromatography for analysis of steroids, alkaloids, bile acids,pesticides, etc, GAS-CHROM Q is DMCS-freafed specifically for such applications. NEED PROOF? Consider the difficult separation of cholesterol fromcholestanol on a low-loaded OF-l column, which is a good test of a support. Itrequires high efficiency and negligible tailing. Dehydration of cholesterol canoccur if the support is not made just right. Q is made just right as shown infigure 1. Results for the same test mix on similar OF-l columns made withother commercially available silane-treated supports are shown in figures 2and 3. Note any difference? The test mix contains cholestane as a reference.A good thing, too. Wonder where the -erol and -anal went in figures 2 and 3?look at the baseline after the cholestane peak.

r.~FiGUR~E-ll

·-"-l~ ;'~URE 31FIGURE 2

~-~...... ~ !-f- 2

.:0,...... o !,.,/ "- I.... "4 ~ ~

I

FIGURE l.FIGURE 2.FIGURE 3.

GAS·CHROM Q, 100/120 mesh, 7.9 grams of packingCompetitor A, 100/110 mesh, 7.8 grams of packingCompetitor B, 100/120 mesh high density white, 14.4 grams

All columns were 6 ft. x 4 mm ID glass U tubes run in a Barber Colman Model5000 at 230· C. Packings consisted of 3% (or equivalent) of OF-1.

PRICES: Mesh60/8080/100100/120

$/100 g26.0030.0031.00

APPLIED SCIENCE LABORATORIES, INC.P. O. BOX 140, STATE COllEGE 5, PENNA.• PHONE 814·238·2406

Circle number 3.

8A J. of G. C.-January, 1965

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D~lJ[Ki [Q)~~D~@THE AUTOPREP 705with flame ionization detector

A companion to theAutoprep 700, an efficientand practical preparativegas chromatograph

Some applications ...

• Cis, trans isomers

• Structural isomers

• Azeotropes

• Ultra-pure standards

• Synthetic intermediates

• Natural products

• Trace components

Fig. 1 Steroids1. Androsteronn2. Testnstcrone3. Cortisone4. Pto!:f'stf'rone

Fig. 2 Garlic Sulfides

1. Meth allyl di2. Di allyl di3. Muth al lyl hi4. Allyl tris. Di allyl tri

Fig. 3 Isobutyl disulfide contaminant

PREPARATIVE SEPARATIONS WITH THE AUTO PREP 705

Model 705 gas chromatograph was developed to fillsome spec ific areas of preparat ive gaschromatographynot entirely answered by the popular model 700.

705 uses [ow cost nitrogen as a carrier gas insteadof expensive helium. It also allows the collection ofsmall quantities of components, normally not detectedby a thermal conductivity cell.

The specially developed splitter ensures linear splitting of even high molecular weight samples, A stableelectrometer and excellent temperature controls, to-

gether with the use of the proven 700 components,completes this outstanding new Aerograph.

Model 705 does not replace the Model 700 but isits useful companion. Like the 700, it can be usedpreparatively or analytically.

Write for details on how to-ORDER

705-AEROGRAPH AUTOPREP with flame detector700-AEROGRAPH AUTOPREP with T. C, detector515-Flame Kit for AUTOPREP 700 complete with

flame head, base, cables and electrometer

WILKENS INSTRUMENT & RESEARCH, INC.P. O. BOX 313 • WALNUT CREEK, CALIFORNIA • PHONE: 415-939-2400

Circte number 4.

J. of G, C.-January, 1965 7A

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SA J. of G. C.-January, 1965

NOWGET YOUR CHOICE

OF20GAS CHROMATOGRAPHS

TO MATCH YOURSPECIFIC

ANALnlCAL PROBLEMS

MODELNUMBER

820-1

820-11

820-111

820-IV

820.V

820-VI

810-1

810-11

810-111

810-IV

810-V

8T O-VI

8TO-VII

810-VIII

810-IX

810.X

81.1-1

811-1f

811.111

811-IV

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Perkin-Elmer Models 810, 811 and 820 Gas Chromatographs offer

you a total of twenty different high-performance instruments, with

the most-desired advantages of gas chromatography, at remark-

ably low prices. They provide differential detectors, dual injectors

and dual column operation for maximum baseline stability under

programmed-temperature conditions, as welt as the versatility

of an additional column to be operated as suits the analytical

requirements.

With a choice of detector types to suit the requirements of any

laboratory; ballistic, and linear-temperature-programming, and

Circle number 6.

isothermal operation in either single or dual column mode, to

350°C; a variety of standard columns, packed or open tubular-

with these features you get compact, laboratory-bench packaging

and versatility of application to an extended range of problems.

And each of these instruments costs far less than comparable instru-

ments with similar capabilities. The table below outlines the spe-

cifics of each.

For further details on these twenty low-colt instruments, and other

Perkin-Elmer Gas Chromatographs, write to Instrument Division,

Perkin-Elmer Corporation, 861 Main Ave., Norwalk, Connecticut.

DEKKIN-ELMEIR

MODEL 810— MODEL 811 — MODEL 820 GAS CHROMATOGRAPH — COMPLETE INSTRUMENT COMBINATIONS

COLUMNS TEMPERATURE MODEL INSTRUMENT INCLUDES AND INJECTORS

DETECTORS CONTROL NUMBER

BASIC MODEL 820 WITH

ISOTHERMAL

AND

820-1

Hot Wire Power Supplya Restrictor for Approximate Flow Control 820-11

Plot Wire Power Supply • Dual Differential Flow Control MODEL tOX

THERMAL

BALLISTIC

PROGRAMMING

820-111

Hot Wire Power Supply s Dual Differential Flow Control nFully Proportional Temperature Control for Detector

CONDUCTIVITY 820-IV

DUAL DETECTORS

Hot Wire Power SupplyuDual Differential Flow ControlISOTHERMAL AND 820-V

METALLINEAR PROGRAMMING FULLY PROPORTIONAL

Wire Power Supply uDual Differential Flow Control n TEMPERATURE 820-VIFully Proportional Temperature Control for Detector COLUMN, CONTROL

'BASIC MODEL 810 WITH 810-1DUAL

ISOTHERMAL ANDInstallation Kit for Carrier & Fuel Gases n

Restrictor for Approximate Flow Control INJECTION BALLISTIC 810-II

DIFFERENTIAL PROGRAMMING

Dual Differential Flow Control PORT FLAME 810-111

DETECTORSDual Differential Flow Control INSTRUMENT ISOTHERMAL AND 810-IV

LINEAR PROGRAMMING

Dual Differential Flow Control n Effluent COMBINATIONS FULLY PROPORTIONAL 810-VSplitter Restrictor Kit for Collection TEMPERATURE CONTROL

Instrument With Hot Wire & Flame Detectors 810-V1ISOTHERMAL AND

Installation Kit for Carrier & Fuel Gases n Hot Wire Power Supply MODEL I0X

THERMAL

BALLISTIC

PROGRAMMING

810-VU

Hot Wire Power Supply t Dual Differential Flow Control CONDUCTIVITY

AND DIFFERENTIAL

810-VIII

Hot Wire Power Supply a Dual Differential Flow ControlISOTHERMAL AND 810-IX

FLAME COMBINATION LINEAR PROGRAMMING

Hot Wire Power Supply • Fully Proportional Temperature Control FULLY PROPORTIONAL810-X

for T/C Detector • Duel Differential Flow Control TEMPERATURE CONTROL

BASIC MODEL 811 WITHDUAL GLASS

ISOTHERMAL AND

BALLISTIC

811-1

Installation Kit for Carrier & Fuel Gases nCOLUMNS AND PROGRAMMING 811 -It

Restrictor for Approximate Flow ControlDUAL DISPOSABLE

DIFFERENTIAL

FLAME

DETECTORSDual Differential Flow ControlISOTHERMAL AND 811-111

GLASS INJECTION LINEAR PROGRAMMING

Dual Differential Flow Control • PORTS FULLY PROPORTIONAL 811-IVEffluent Splitter Kit for Collection TEMPERATURE CONTROL

J. of G. C.—January, 1965 9A

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new from Pye

the brilliant series 104 chromatographsdual flame .•.

katharometer •••

temperature programmed •••

choice of .. models •••

The new pye 'Series 104' Gas Chromatographs comprise abasic oven unit with a range of analyser heads, each with adifferent detector and column specifically designed for aparticular range of work. This provides the chromatographerwith an instrument system of maximum versatility and breadth ofapplication whilst preserving the high Instrument performanceof a specific analyser.The pye 'Series 104' gives faster analysis with a warm-up rate ofup to 48°C per minute and an operating temperature as high as500°C, easier analysis due to the careful design of all operatingcontrols and large, easily accessible oven, and cheaper analysiswith outlay and operation costs lower than any other system ofcomparable performance.

--- ~ -----_.. "- -_. --- _._-----------MODEL Aame lonlsatlen Isothermal Chromatograph In-

4eorporatlng analyser oven, single flame lonlsatlon

, deteetor head unit, propo rtlonal ternperature con-troller and wide range lonlsatlon amplifIer.

MODEL Flame 10nlsatJon Temperat~re Programmed

14Chromatograph whleh Is similar to Model 4 butfitted with a temperature programmer/eontrollerwhleh has several new design features.

MODEL Dual Flame IonisatIon Temperature Programmed

24Chromatograph Ineorporatlng analyser oven, dualflame Ionisation detector head unit. temperatureprogrammer/controller and wide range Ionisationamplifier.

MODEL Katharometer Isothermal Chromatograph Incor-

34 poratlng analyser oven, katharometer detedorhead unII, propertlonal temperatura eontroll arand katharometer power supply unit.

Write for descriptive brochure to W. G. Pye & Co. ltd., P.O. Box 60,York Street. Cambridge, England. (Telephone: 58866)Clrefe number 7.

10K J. of G; C.-January, 1965

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PUT THIS

ADVANCED UNIT

TO WORK

IN YOUR

LABORATORY

LOENCOMODEL I&OPMT- DUAL TC DET.MOD.EL I&OPMF-DUAL FID

A NEW CONCEPT IN COMPACT, FLEXIBLEAUTOMATIC GAS CHROMATOGRAPH INSTRUMENTATION

The Model 160 Prep~Matic

Instrument is an advanced automatic facility combining the bestin both preparative and analytical gas chromatography.

PREPARATIVE MODE-Completely automatic separation ofsamples up to 3 cc-collects 8of first 20 peaks above threshold-"anti-dribble" injection system prevents sample bleed.Glass columns available-"on-

column" or standard injection.Patch board collection programmer. No nonsense with mechanical indexing of collection tubes.Controlled temperature programming.

ANALYTICAL MODE-Temperature programmed or isothermal analytical separations withhigh performance detection systems. Remember, you can purchase the Prep-Matic with either

thermal conductivity or hydrogen flame ionization detection.Pre-analyse your prep samples,check purity of separated samples. Do any analytical work.Also remember that the "singlecycle automatic" position of theprogramming system automatically programs the temperature,blows oven down and re-establishes initial conditions ready forthe next run. It will pay you to-

SEND FOR THIS DESCRIPTIVE BROCHURE

Get the complete story about this remarkable unit. A comprehensivefour-page, two-color brochure is now available without charge. Itscharts, diagrams, photographs and salient performance facts willpermit you to carefully study the full worth of the Prep-Matic unitto you. Send for a copy.

LOENCO inc.c:r&ucurc:ed'~.Aunte/df fii e;d?EP/l-rC

2092 NORTH LINCOLN AVENUE I ALTA DENA. CALIFORNIA I 794-1167 (AC 213)

Circle numeera,

J. of G. C.-January, 1965 11&

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Phosphorous-containing compounds are one of the mostrecent groups to yield to a Packard Gas Chromatograph.The chromatogram which is shown above was made ona Packard Model 7611 dual system (dual column oven,dual detectors, dual electronics and dual recorders) andrepresents an important achievement in simultaneousdetermination of compounds of widely separated con-centration.

A modified flame ionization detector with a sodiumemission grid and termed a SODIUM THERMIONICDETECTOR (STD) bas beenfound to be nearly 1000 timesmore sensitive to phosphorous-containing pesticides thanthe standard flame ionizationdetector (FID). The samplewas separated on a single col-umn and passed through a 1:1ratio stream splitter beforesimultaneous detection in thenormally sensitive FID and thehighly sensitive STD. In thismanner, all the organic materials were detected in theFID (upper curve) while the trace amount of phospho-rous compounds was readily detected in the STD unit(lower curve).

Packard Gas Chromatographs offer many significantadvantages to research workers in the biochemical andbiomedical disciplines. Fast, stable, highly sensitivedetermination, versatility and convenience of operationare some of the reasons why you should know moreabout these superb instruments.. Your Packard SalesEngineer can provide complete details and performancecriteria. Write for Bulletins and Specifications.

EXPERIMENTALCONDITIONS*

1 lindane1 ug parathion.2 µg methyl stearate

COLUMN: 5 ft. x 4 mm all glass

LIQUID PHASE: 10% Dow Corn-ing Silicone Fluid (DC200)

SUBSTRATE: 80 -90 mesh Ana-crom ABS (acid and basewashed, and silanized)

CARRIER GAS FLOW RATES:Nitrogen 60 cc /minHydrogen FID 40 cc /min; STD60 cc /minAir 300 cc /min

TEMPERATURES:Inlet Heater 225 °CColumn 205 °CDetectors 200 °COutlet 200 °C

DETECTORS: Standard PackardHydrogen Flame lonization(FID); Modified Packard loni-zation Detector with sodiumemission grid (STD) .

ELECTROMETER RANGES:FID 1 x 10-9 amperes fullscaleSTD 3 x 10-7 amperes fullscale

DETECTOR VOLTAGE: 300 volts

NOISE LEVEL: 1 x 10' amperesfull scale

CHART SPEED: 30 inches/hour

•L. Guiffrida, J.A.O.A.C., 47, No. 2,293 (1964)

1 Packard 1 PACKARD INSTRUMENT COMPANY, INC.

BOX 428 • LA GRANGE. ILLINOIS • AREA CODE 312.485-6330

Circle number 9.

12A J. of G. C.—January, 1965

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BARBER-COLMAN GAS CHROMATOGRAPH

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