.. ;'.'

No.

~.._.... \.~,.'.'..~-:"'.".,- .'.'~."..,., ...,' .'.'.'..'..•.;."' .._.,'~'/.":~", .'.'.', ',".'. " .

9200166

Publicreporting burden for. this colleclJonof information isestimated to average30.•••inutesper response.including the time for r~iewi"jl instructions.searchingexisting data sources.gathering andcmaintaini"!1the data needed,and completing and reviewing the collectionof information. Sendcommentsregarding tho>burden estimate or any.other aspect of.thiscollectionof information, •••duding suggestionsfor redudng this burden, to Departmentof Agriculture,OearanceOffice,OIRM,Room404.W.Washington,D.C.20250;'andto the Officeof ManagementandBudget...••aperwork ReductionProject(OMB#0581-0055),Washington, 20250. fORM APPROVED:OM80581-0055,Expires113.1191

13. NAME ANDADDRESSOFAPPLICANTREPRESENTATIVE(S),IFAttY, TOS;WRVEIN HISAPPLICATIONANDRECEIVEAll PAPERS I"!...LJ - t7P-rl (fE.. 'D ~K. H. Quesenberry' J\,( Jj)"'~S, ,¥.Jil;:: J:bp.mp-8't)fi, i\st;iug Dean for Research

IFAS 0152, University of Florida ~ ./ 1022 McCarty Hall, University of FloridaGainesville, FL 32611 ~fJ-' rr-k>¥ainesville, FL 32611

')",,'A.If~ I ~ PHONE(Include area code 2. - l =tg414. CHECKAPPROPRIATEBOXFOREACH.ATTACHMENTSUBMITT£D(Follow INSTR on r<werse)

a. lXl Exhibit A, Origin and Breeding History o( the Variety. I tl'j"b. [X) Exhlbil B, Novelty Statement.

e. 00 Exhibit C, Objective Description o( Variety.

d. 00 Exhibil D. Additional Description o( Variety.

e. [X) Emibit E, Slatement 01 Ihe Basis 01 Appticanrs Ownership.

I. [X) Seed Sample (2.500 viable untreated seeds). Dale Seed Sample mailed 10Planl Variety Protection Ollice April 16. 1992g. 00 Filing and Examination Fee ($2,150) made payable to "Treasurer 01 the United States."

15. DOESTHEAPPLICANT(S)SPECIFYTHAT SEEDOFTHISVARIETYBESOlO BYVARIETYNAMEONLYAS A ClASS OFCERTIFIEDSEED?(See section 83(a) o( (he Planl VarietyProtection AcI.) 0 IVl

. YES (If "YES." answer items 16and 17below) ~ NO (If -NO, - slip 10item 18 below)

u.s. DEPARTMENTOFAGRiCUlTUREApplication is required in order toAGRICULTURAlMARKETINGSERVICEdetermine il a plant .variety protection

APPLICATION FOR PLANT VARIETY PROTECTION CERTIFICATE certilicale is 10 .be issued (7 U.S.C. 2421).Intormation is held confidential until

(Instructions on reverse) cerlilicale.1s issued (7 U.S.C. 2426).

1. NAME OFAPPLICANT(S)(as it is to apPlIar on tha Certificate) 2. TEMPORARYDESIGNATIONOR 3. VARIETYNAME

Agricultural Experiment Station EXPERIMENTALNO.

University of Florida FL5 Cherokee4. ADDRESS(street and no. or'RF.D. no., city, state, and ZlP) 5. PHONE(Include area code) FOA OFFICIAL USEONl YOffice of Dean for Research PVPONUMBER

1022 McCarty Hall, University of Florida 904-392-1823 C(2oCJ lC>bGainesville, FL 32611F DaleI ~:-_2:.!L~!!!:_l

6. GENUSANDSPECIESNAME 7. FAMILYNAME (Botankaf) I TimTrifolium Leguminosae N o A.M. o P.M.

pratenseG

CROPKINONAME (Common Name) F Filing and Examination Fee:8. 9. DATEOFDETERMINATION E ~~.-L~~~~ _______1991 ERed Clover August 1, s(L10. IF THE APPLICANTNAMEDIS NOTA "PERSON: GIVEFORMOFORGANIZATION(Corporation, partnership. associalion. ele.) R J-o", .2g/9'1Z.University of Florida Agricultural Experiment Station ECerlfricale Fee:- C

E s3lJO. aDII. IF INCORPORATED.GIVESTATEOF INCORPORATION 12. DATEOF INCORPORATION I -----------------V DaleN/A N/A E FEB .. /:. 199~0 .

o CERTIFIEDo REGISTEREDo FOUNDAnol~

; 17. IF "YES" TOITEII..\5, WHICHCLASSESOFPROOUCTlONBEYONDBREEDERSEED?II11

16. DOESTHEAPPlICANT(S) SPECIFYTHATTHISVARIETYBE LIMITEDAS TONUMBER'OFGENERATIONS?

o YES 0 NO

-----------------------------~. -------------------~-------------18. 010THEAPPLICANT(S)PREVIOUSLYFILEFORPROTECTIONOFTHEVARIETYIN THEU.S.'

o YES (If "YES. "Ihrough 0 Plant Variety PrOlection Ael 0 Palenl Ael. Give dale: .)

pNO ~~fh~ AM- z.~J4qr;'19. HAS THEVARIETYBEENRELEASED,USED,OFFEREDFORSALE,OR MARKETEDIN THEU.S.OROTHERCOUNTRIES'

I:KI YES(II "YES: give names01counlries and dales) United States, October 10, 1991ONO

IDATE

Acting Dean for ResearchCAPACITYORTITLE

CAPACITYORTITLE

20. The applicant(s) declare(s) that a viable sample of basic seeds ofi1.is variety will be fur:"ished with the application and will be replenished uponrequest in accordance with such regulations as may be applicable. ..

The undersigned appllcanUs) is (are) the owner.(s) of this sexually reproduced novel plant variety. and believe(s) that the variety is dislinct,uniform. and stable as required in section 41 ~and'is entitled to protection under the provisions of seclion 42 of the Plant Variety Protection Act.

ApplicanUs) is (are) informed that false representation herein can jeopardize protection and result in penalties.

FORMCSSD.470(5-&9) EdOl,ou01 FORMLS.470. ).116.os""'s,,ldc

INSTRUCTIONS

GENERAL: To be:effectively filed with the Plant Variety Protection Office (PVPO), ALL of the following items mustbe received inthePVPO: (1) Completed application form signed by the owner; (2) completed Exhibits A,B,C,E; (3) atleast 2,500 viable untreated seeds; (4) check, drawn on aU .S. bank, payable to "Treasurer of the United States" in theamount of $2,150($250 filing fee and $1,900 examination fee). (See section 180.175 of the Regulations and Rules ofPractice.) Partial applications will be held in the PVPO for 30 days, then returned to the applicant as unfiled. Mailapplication and other requirements to: Plant Variety Protection Office; AMS, USDA, Rm. 500,NAL Building, 10301Baltimore Blvd.,.Beltsville, MD 20705-2351. Retain one copy for your files. All items on the face of the Applicationare self-explanatory unless noted below. Corrections on the Application form and Exhibits must be initialed and dated.DO NOT use masking materials to make corrections. If a Certificate is allowed, you will be requested to send a checkpayable to "Treasurer of the United States".in the amount of $250 for issuance of the Certificate.

ITEM9.

14a.

14b.

14c.

14d.

14e.

15.

19.

Plant Variety Protection Offic.eTelephone:! 3'01/504-5518

Give the date when there has been at least a tentative determination that the variety has been sexuallyr.eproducedwith recognized characteristics, whether or not the novelty of those characteristics has beendetermined. (See section 41(d) of the Plant Variety Protection Act (Act).]

Give: (1) the genealogy, including public and commercial varieties, lines, or clones used, and the breedingmethod; (2) the details of subsequent stages of selection and multiplication; (3) the type and frequency ofvariants during reproduction and multiplication and state how these variants may be identified and (4)evidence of uniformity and stability. (See sections 41 and 52 of the Act.)

Give a summary statement of the variety's novelty. Clearly state how this novel variety may be distinguishedfrom all other varieties in the same crop. If the new variety most closely resembles one or a group of relatedvarieties: (1) identify these varieties and state all differences objectively; (2) attach statistical data forcharacters expressed numerically and demonstrate that these are clear differences; and (3) submit, if helpful,seed arid plant specimens or photographs of seed and plant comparisons which clearly indicate novelty.

Exhibit C forms are available from the PVPO; specify crop kind. Fill in the Exhibit C (Objective Description ofVariety form) to describe your variety.

Optional additional characteristics and/or photographs: Describe any additional characteristics that cannot beaccurately conveyed in Exhibit C. Usc comparative varieties as is necessary to reveal more accurately thecharacteristics that are difficult to describe, such as plant habit, plant color, disease resistance, etc.

Section 52(4) of the Act requires applicants to furnish a statement of the basis of the applicant's ownership.The applicant may be the actual breeder, the employer of the breeder, the owner through purchase orinheritance, etc. " .

If "Yes" is specified (seed of this var;pty be sold by variety name onl)' as a cla.ss of certified seed), the applicantmay NOT reverse this affirmative decision after the variety has either been sold and so labeled; the decisionpublished, or the certificate issued. How~ver, if "No" has been specified the applicant may change the choice.(See section 180.16 of the ReguLations and RuLes of Practice.)

See sections 41 (i, j) and 42 of the Act and section 180.7 of the Regulations and Rules of Practice for eligibilityrequirements,

NOTES:It is the responsibility of the applicanUowner to keep the PVPO informed of any change of address or change ofownership or assignment during the life of the application/certificate. There is no charge for filing a change ofaddress. The fee for filing a change of ownership 9r assi~ment i,S$25. [See s~,c,.~O,,1l'~l,JJ1, d(l?z~-Act" ,and sections180.130,180.131,180.132, and 180. 175(h) of the Regulatlons and Rilles ofPrac!ice)] ".,T 'd fl' . h h' . h I' h ld h/,Y:'th R~r.!:"tre~ 0\, 'd bo avOl. con' lct Wit ot er vanety names 10 uSe, ~ e.app lcant s o~c ..•e~K el.xane .y,~namet.B!'fpose ycontacting: Seed Branch, AMS, USDA, Rm. 213, BUlld10g306, Beltsvllle~gnculturaIResen$ch CeXltc\r__East,

Beltsville, MD 20705. Telephone: 301/504-8089 l.' ',. MA,Y 1 ~91~- 21992 t> J~!- F..". . I i./ P ,Ctl.;ty /-/(1- t\;.o;)l.:;oo Ofe. '~/~ '

~rrr:-:\

---~--~-----~~--------;;-T~~-----------~--------

14a. Exhibit A. Origin and breeding history of Cherokee red clover

The original population from which Cherokee was selected consisted of approximately 12% ,Nolins' ,14% 'Tensas' ,and 74% reseeding selections from evaluations of plant introductions at Gainesville andFt. Pierce, FL in 1976, 1977, and 1978. A bulk of this seed (cycle 0 seed) was planted in rows on theAgronomy Farm at Gainesville in fall 1978 and seed was harvested from the most vigorous, earlyflowering plants in spring 1979 (cycle 1). Similar planting and selection methods were carried out in1979-80 (cycle 2). Beginning in fall 1980, seedlings were established in flats in a greenhouse and thentransplanted to the field planted on 30 cm spacing in 90 cm rows. Plants grew through the winter,were mowed off in mid-March, and allowed to regrow. The most vigorous and early flowering 20%of plants were allowed to intercross after removal of the remaining plants. At seed harvest, allselected plants were under-cut with a peanut lifter and root systems were classified as either badlygalled due to infestation by root-knot nematodes (Meloidogyne spp.) or partially resistant with reducedgalling. Seed from 616 low-gall plants was bulked (cycle 3). A similar process was carried out in1981-82 (cycle 4) and 1982-83 (cycle 5) except that plants were not undercut and classified fornematode galling. Selection pressure among cycles varied between 10 and 20%. Numbers of plantscontributing to the various selection cycles ranged between ca. 500 and 1000. In fall 1983, anapproximate 0.1 ha field at the Green Acres research farm near Gainesville was direct seeded in rowsusing cycle 5 seed. No selection pressure was imposed on this planting and seed harvested from thisfield in spring 1984 became the initial breeder seed of Cherokee. Foundation seed was produced inCalifornia in 1990 and certified beginning in 1991. We have observed progeny plants from breeder,foundation, and certified seed lots, and the variety is stable and uniform over these seed increasegenerations. Certified seed lots retain all novel characteristics of the variety. No variant plant typeshave been observed in this cultivar other than the normal variability for stem hairs, leafmarking, flowercolor, and seed color described in items 7, 8, 9, and 10 of the original application.

2

i z: 6 \j Z- N~r 96.

920016614b. Exhibit B. Novelty Statement

Cherokee is a semi-nondormant red clover with most plants initiating flowering stems in daylengths ofless than 12 h. Under typical spring growing conditions in Florida, Cherokee will flower 14 to 21 dearlier than ,Kenstar' . The only other known red clover germplasm with similar nondormantcharacteristic is the cultivar Nolins which is no longer in commercial production. Cherokee has lower(p < 0.05) root galling and nematode reproduction (egg mass score) in response to the root knotnematodes Meloidogyne arenaria (Neal) Chitwood, M. hapla Chitwood, M. incognita (Kofoid andWhite) Chitwood, and M. javanica (Treub) Chitwood than other released cultivars or germplasms ofred clover (3). Evaluations of over 300 other red clover plant introductions and cultivars have not anywith as high level of resistance to root knot nematodes as Cherokee (Table 1 below).

Table 1. Response of red clover lines to root knot nematodes.

Population

M. arenaria

Gall Egg

M. incognita

Gall Egg

M. javanica

Gall Egg

- - - - - - - - - - - - - - - mean score t - - - - - - - - - - - - - - - - -

CherokeeKenstarPI Mean+LSD (0.05)

3.74.64.60.3

3.44.64.80.3

3.44.84.70.3

2.74.44.60.4

2.94.34.50.4

2.64.24.70.4

t Rated on a scale of 0 = 0 galls or egg masses. 1=1 or 2, 2=3-10, 3=11-30,4=31-100,5=>100.

+Mean response of 150 plant introduction lines.

3

-- ~-----~-----~-~~----------------------------~-------------~'..,-

U.S. DEPARTMENT OF AGRICULTUREAGRICULTURAL MARKETING SERVICE

LIVESTOCK, POULTRY, GRAIN & SEED DIVISIONBELTSVILLE, MARYLAND 20705

FORM APPROVED: OMB NO. 40-R3822

EXHIBIT C(Red Clover)

CherokeeVARIETY NAME OR TEMPORARY DESIGNA-TION

OBJECTIVE DESCRIPTION OF VARIETYRED CLOVER (Trifolium Pratense)

Agricultural Experiment StationUniversity of Florida

NAME OF APPLICANT(S)

ADDRESS (Street and No., or R.F.D. No., City, State, and ZIP Code)

Office of Dean for Research1022 McCarty Hall, University of FloridaGainesville. FL 32611

FOR OFFICI~L USE ONLY

PVPO NUMBER

9200166Place the appropriate number that describes the varietal character of this variety in the boxes below. F ill unused columns with zeros (e.~. I 0 I 9 191when number is 99). In comparisons to standard varieties, the value LQOlJi] should only be used to indicate that the varieties ar~_equal. Thesymbol A indicates a decimal point. Characteristics described, includin~ numerical measurements, should represent those which are TYPICAL forthe variety. Measured data should be for SPACED PLANTS. Any recognized color fan, e.g. Royal Horticultural Colour Chart, may be used to deter-,mine plant colors; designate system used: . Give location of test area _

Ranges of values are valuable and may be included with additional description elsewhere in the application.

NOTE: For single plant data a minimum of 100 plants is suggested.

1. TYPE:

[U 1 = DOUBLE CUT (medium) 2 = SINGLE CUT (mammoth) 3 = OTHER (Specify) _

2. PLOIDY:

IIIL_~ 1 = DIPLOID 2 = TETRAPLOID 3 = OTHER (Specify) _

3. PRODUCTIVE PERSISTENCE (Usual duration of planting):

1 = ANNUAL 2 = BIENNIAL 3 = SHORT LIVED PERENNIAL (3 - 4 Years)

4. ADAPTATION: (e.g" ~ = northcentral and southcentral)

1 = NORTHEAST

4 = SOUTHEAST

2 = NORTHCENTRAL

5 = WEST

3 = SOUTHCENTRAL

6 = OTHER (Specify) _

STANDARD VARIETIES

2 = ARLINGTON 3 = PENNSCOTT1 = KENSTAR

5. MATURITY:

~ % PLANTS FLOWERING IN SEEDLING YEAR

4 = TENSAS 5 = AL TASWEDE

Beginning of spring growth:

w=Q] DAYS EARLIER THAN" ••••••••••• , •• ".,." [i]~ DAYS LATER THAN ••••••••••••••••••••••••• D

STANDARD VARIETY

STANDARD VARIETY

Time of flowering (50% of plants in bloom): (from spring growth in non-seedling year)

Ci:IJ DAYS EARLIER THAN ••••••••••••••••••••••• [J STANDARD VARIETY

~ DAYS LATER THAN ••••••••••••••••••••••••• D STANDARD VARIETY

6. PLANT HEIGHT (from soil level to top of flowering haad at 50% flowering)

~ CM. TALL ~ CM. SHORTER THAN

WJ CM. TALLER THAN

FORM LPGS-470-42 (8-80) (Formerly Form GR-470-42 (9-77) which may be used)

WD

STANDARD VARIETY

STANDARD VARIETY4

920016614<1.Exhibit D. Additional Description of Cherokee red clover

Cherokee is a semi-nondormant red clover selected and developed for the lower southeastern UnitedStates. Most plants of Cherokee will initiate flowering stems on daylengths of less than 12 h.Cherokee is generally adapted to the southeastern United States as far north as Tennessee and westuntil persistence becomes limited by annual rainfall. In most of these areas it persists as abiannual/perennial, but in peninsular Florida it is grown primarily as an annual. In cultivar evaluationtrials across the lower coastal plains, Cherokee has shown superior early season dry matter (DM)yields compared to other red clover cultivars. It has yielded less than crimson clover at early harvestsbut produced higher total season yields because of persistence later into the season, and wascomparable in yield to 'Florida 77' alfalfa (Medicago sativa L.) during the spring months. At thesesouthern locations, Cherokee was usually superior in DM yield compared to more dormant red clovercultivars which were developed in areas to the north.

Cherokee has an increased level of tolerance to root knot nematodes Meloidogyne arenaria (Neal)Chitwood, M. hapla Chitwood, M. incognita (Kofoid and White) Chitwood, and M. javanica (Treub)Chitwood compared to any other released cultivar or germplasm of red clover. Cherokee is similar toother released cultivars in response to northern anthracnose [Kabatiella caulivora (Kirchn.) Karak.],southern anthracnose (Colletotrichum trifolii B & E) and aphanomyces (Ahpanomyces euteichesDrechs.).

7

,--

9200166Exhibit F. Documentation of partial resistance of Cherokee red clover to root-knot nematodes

Quesenberry, et al (1986) have published methodology for screening Trifolium spp. forresponse to root-knot nematodes. Using this method, we have demonstrated that Cherokee(FL5) has lower gall and egg mass scores in response to root-knot nematodes (Meloidogynespp.) than the standard check cultivar, Kenstar (Quesenberry, et aI, 1989). We have alsoscreened over 150 plant introductions from the U.S. National Plant Germplasm System forresponse to root-knot and have not identified any lines with as low root galling and egg massscores as Cherokee. A summary of these results is presented in Table 1 of Exhibit B.

Quesenberry, K. H., D. D. Baltensperger, and R. A. Dunn. 1986. Screening Trifolium spp. forresponse to Meloidogyne spp. Crop Sci. 26:61-64.

Quesenberry, K. H., D. D. Baltensperger, R. A. Dunn, C. J. Wilcox, and S. R. Hardy. 1989.Selection for root-knot tolerance in red clover. Crop Sci. 29:62-65.

8

,-------- ---

9200166

14e. Exhibit E. Statement of the Basis of Applicant's Ownership

The cultivar for which Plant Variety Protection is hereby sought (Cherokee red clover) wasdeveloped by Dr. K. H. Quesenberry, an employee of the University of Florida, Institute ofFood and Agricultural Sciences (IFAS), Agricultural Experiment Station, AgronomyDepartment. By agreement between the employee and the University all rights to any cultivardeveloped by the employee while employed by IFAS were assigned to the FloridaAgricultural Experiment Station

9