praktikum biokim 6

7/24/2019 praktikum biokim 6

1/18


2/18

BAB I

PENDAHULUAN

1.1. Latar Belaa!"

L!"# "$"%"& '" '*+"" "" !-%#% +"*. *# $" "'"!

%!"# $"* .%'-% L!"# * "#"* &$-%'' !*3"$ "'"! %!"# $"* .%'-%

$*."* !*..*"#"* %""* "%#"% L!"# +"*. "'"% $" &"*, $"* %!"#

'" 3*& +"*. !"#"* "$""* %*"# "$" '& "*."* $"* !*+"#

"'"% $" !&"* (G*$", 1660)

L$ 7*$*. %" $"%"! %" -."*# !'"%*+" $"* #%---!

'" !"#"* '*+"" +"*. &-.* $" 3"*."* S" * +"*.

!!$"#"* $*."* #"-&$", -*, "'"! *#%", $"* #"*+"#"* !-%#%

&"+" %"**+" (G*$", 1660)

A$"* .%'-% +"*. $-%& $" &"'% *+"*"* %!"# "" !*+"#

"$"%"& '" 8" 7" +"*. $"# "*" $"* !!*+" "'" +"*. "."# !"*'

G%'-% %" "# $"%"! " $"* $"# %" $"%"! A"%" .%'-% $7"!

$*."* KHSO4$"* $"*"'#"* &"9&", "#"* !% " +"*. "3"! #&"' '

" %!"# +"*. "#" $'"#"* -%& *#*+" "#%"%$&$" ""

"#-%* O%& #"*" !%*+" " +"*. "3"! , "#-%* !$"& $#"& $"*

"#'*+" $3"$#"* *# !**#"* "$"*+" .%'-% "" '*+"" +"*.

!*."*$*. .%'-% ' %!"# $"* !*+"# (P-$3"$3, 1664)

B$"'"#"* ""* $""', !"#" $%"##"* 7-""* * "." !"&"''"

$"" !*."& !-$ 3 %$ !%"% ' "#-%* $"* ' #-%-! *#

!**#"* #"*$*."* .%'-% "$" %$ S%"* , "." !"&"''"

!!-%& #"!%"* !*.*" 3 %$


3/18

1.2 Ma#$% %a! T$&$a! Per'()aa!

1.2.1 Ma#$% Per'()aa!

A$"* !"#'$ $%"##"**+" 7-""* * "$"%"& *# !*."& $"*

!!%"3" 3 %$ !%"% ' "#-%* $"* ' #-%-!

1.2.2 T$&$a! Per'()aa!

T3"* "##! * +" :

1 U*# !**#"* "$"*+" .%'-% $"%"! %$ !%"% ' "#-%* +"*.

$"*$" $*."* !%*+" " *.#

2 U*# !**#"* "$"*+" .%'-% $"%"! %$ !%"% ' #-%-!

+"*. $"*$" $*."* !*#"* #-!%#' &3" 8"!$

1.* Pr+!#+, Per'()aa!

1.*.1 Te# Ar(le+!

T' * $%"##"* $*."* !"#'#"* %""* 7-*-& $*."* KHSO4

#!$"* $"*"'#"*, $"* &"'% -' 3#" '"!% " *.#

1.*.2 Te# K(l(r+-etr+

T' * $%"##"* $*."* 7"" !"#'#"* %""* 7-*-& $*."* N"O%

2 ;, H% #!$"* $$$"* %"% $"!""* 9*"-% $"* H 2SO4$"* &"'%*+"

-' 3#" '"!% !!*# #-!%#' &3" 8"!$

1. Ma!/aat

M"*"" $" 7-""* * +" !"&"''" $"" !!"&"!

!-$ 3 %$ !%"% ' "#-%* $"* ' #-%-! *# !**#"*

#"*$*."* .%'-% $"%"! %$


4/18

BAB II

TINJAUAN PUSTAKA

L$ "$"%"& '"%"& '" #%"' !-%#% -%-.' #"* '" +"*. $"#

!*7"# -%! '3" S*+""9'*+"" +"*. $' %$ $#%-!-##"*

!*3"$ '" #"*" !!%# '" #'"!""* 7 **., +" %$ '%

7"! $*."* ", ""* !*.#* $"# '" '"!" '#"% P%"# &$--#

* $'"#"* -%& '# !-%#%*+" W"%"* !*.#* !!%# ""

#""* -%" +"*. "'-'"' $*."* -#'.*, %$ '"."* '" $ $"

%"+"& &$-#"-* ("!%%, $##, 200


5/18

"'"% $" !&"* M*+"# '" "# 3*& $"* 3$ 7" "$" '&

"*."* (G*$", 1660)

S*+"" -."*# +"*. .-%-*. %$ (N"', $##, 2014) +":

" L%*, !"#"* ' $" "%#-&-% "*" "*3"*. $*."* "'"! %!"#

"*" "*3"*. P"*3"*. "*" &$-#"-* "'"! !"* "%#-&-% "$ %%*

"'"*+" #'" 10 '"!" $*."* =0 #"-* B$"*+" $*."* .%'$"

"$"%"& "&" "%#-&-% "$" %%* "%"& "%#-&-% !-*-&$"

T.%'$" !"#"* '*+"" ' $" "'"! %!"# $"* .%'-% A'"!

%!"# "$"%"& #"-#'%" "*" "*3"*. +"*. !!*+" !!%# 3!%"&

"-! #"-* .*" $"* $"" !!%# '" "" %& #""* "*.#" $"

($"# 3*&)

c. >-'-%$, !"#"* %$ " ' "'"! -'" D"%"! !!"* '%

$"" $" 3*' "!" -'-%$ +" -'-.%'$" $"* '*.-!+%*

$ G%#-%$ +" !-%#% %$ +"*. !*."*$*. #"-&$" "'"*+" $

$" .%" '$&"*" ' .%#-'" "" ."%"#-'"

S-$, +" #%-!-# %$ +"*. "*+"# $3!" $"%"! !&"* $"*

&"* S-$ "# '"*#"*, #"*" "# $"" &$-%'' $"%"! !$"

"'", $" $*."* .%'$" $"* %$ #-!%#'

T*, +" '"%"& '" '*+"" -."*# "&"* "%"! +"*. "*+"# $3!"

$"%"! !"#&%# &$ "!" !&"* T* +"*. %& '$&"*"

$#*"% '"." !*+"# "' K%-!-# '*+"" * "*+"# $.*"#"*

'"." "&"* "# "! $"* -"

L!"# '"%"& '" -!-%#% +"*. !*."*$*. ' .%'$" $"

.%'-% $*."* = "'"! %!"# +"*. #" "$" "*" 3*.*+" A'"! %!"#

+"*. #""* $*."* .%'$" "$" $"'"*+" !"#"* "*" #"-* ()

$*."* ..' #"-#'% (OOH) "$" '"%"& '" 3*.*+" +"*. $"" "#'

(#""*) $*."* !-%#% %"* (T!*"&, 2006)


6/18

A'"! %!"# 3*& +"*. #"*$*. $"%"! !*+"# #%"" !'"%*+",

!"#"* .-%-*."*"*" #"-* '$"*. (Medium Chain Fatty Acid? M>A)

+"*. $ $" &"*+" 12 "-! #"-* +"*. $#" 3*& -%& "-! &$-.*

S$"*.#"* "'"! %!"# +"*. #"*$*. $"%"! !*+"# '"+ (!*+"# #$%",

3".*., 3 *." !""&", 7"*-%" $"* I"*9%"*)


7/18

*.1 Baa! Per'()aa!

B"&"* +"*. $.*"#"* "$" 7-""* * "$"%"& !*+"# #%"", !*+"#

'", !*.", " (%%*), .%'-% 10 ;, "C"$', HL #", 9*"-%, KHSO4,

N"O% 2; , H2SO4, $"* '' -%%

*.2 Alat Per'()aa!

A%" +"*. $.*"#"* "$" 7-""* * "$"%"& "*. "#', ',

'#"%", "# "*., .., *"*."' ", .%"' #, ""*. *."$# $"*

'#" "*.

*.* Pr(#e%$r Ker&a

*.*.1 Te# A'r(le+!

E*"! "& "*. "#' !"'*.9!"'*. $' $*."* 1 !L %""* 7-*-&

+"#* " (%%*), !*+"# #%"", !*+"# '", !*." $"* .%'-% 10;,

#!$"* $"!""* 0,5 . KHSO4 # $"%"! %""* 7-*-&, %"% $"*"'#"*

$*."* *"*."' " T!%*+" " #""#'# !*"*$"#"* "$"*+" .%'-%

*.*.2 Te# K(l(r+-etr+

T3& "& "*. "#' !"'*.9!"'*. $' $*."* 1 !L %""* 7-*-&

+"#* " (%%*), !*+"# #%"", !*+"# '", !*.", $"* .%'-% 10;,

#!$"* $"!""* 1 !L N"O% 2; S%"& 29= !*, $"!""* %".

"" ' H% #" #!$"* $$$"* '%"!" 1 !* *# !!"*.

#%&"* "'"!, #!$"* $"!""* 0,2 !% 9*"-%, #!$"* "" '

H2SO4 #", '%"*3*+" $#-7-# $*."* &"9&" T*#*+" "*" &3"

8"!$ "$" %""* !**3##"* "$"*+" .%'-%

BAB IV

HASIL DAN PEMBAHASAN


8/18

P"$" 7-""* * !!"&"' !*.*" ' "#-%* !*..*"#"* %""*

" (%%*), "#"$', !*+"# #%"", !*+"# '", !*." $"* .%'-% 10; T'

* $%"##"* $*."* 7"" !*"!""* 0,5 !% ."! KHSO4 +"*. *.'

'"." #""%'"- !*#"* .%'-% "$" '"!% +"*. !*."*$*. .%'-%

H"'% *."!""* +"*. $-%& +" %""* !*+"# #%"", !*+"#

'", $"* !*." !*!%#"* " #&"' +"*. " *.# S$"*.#"* "$"

%""* .%'-% 7# " T.%'$" +"*. #"*$*. $"%"! '"!% "#"*

&$-%'' !*3"$ .%'-% $"* "'"! #"-#'%" ##" $"*"'#"*, #!$"*

$''% $*."* -#'$"' .%'-% !*3"$ "#-%* S$"*.#"* "$" " (%%*)

$"# !*.&"'%#"* " #&"' ', &"% * !*"*$"#"* "&" #$" %""*

' $"# !*."*$*. .%'-% #"*" %""* $"# &$-%'' ##"

$"*"'#"* D*."* "$"*+" " #&"' !!##"* "&" $"%"! %""* !*+"#

#%"", !*+"# '" $"* !*." !*."*$*. .%'-%

T"% 1 T' A#-%*

-*-& W"*" P"*"'#"* (B")

G%'-% B**. F

M*+"# #%"" B**. F

L%* B**. K#**."* F

M*+"# S" K**. B**. FF

M*." K**. F

A#"$' B**. 9

K"*."* : FF ? "

F ? 7# "

9 ? $"# "


9/18

A$"* "#' *# ' "#-%* +" :

a. L+l+!

:H= (:H2)14 : O:H2 (:H2)14 :H= F KHSO4

O

). Gl+#er(l

H2:

H:OH

H2:OH

:H

:H

:H2

F 2H2O

O

"#/-%*

KHSO4

'. M+!3a Kela,a Sa4+t

H2: O

CH O

C

H2C O

C

CH

O

(CH2)12

O

(CH2)12O

CH3

CH3

(CH2)12 CH3

F KHSO4

:H2OH

CHOH

CH2OH

F =H=:9(:H2)129:9OH

O

:H2

CH

HC O

("#/-%*)

%. M+!3a Kela,a

e.Me!te"a


10/18

H2C O C (CH2)14CH3

H2C O C (CH2)14CH3

HC O C (CH2)14CH3

O

O

O

H2C OH

HC OH

H2C OH

+ H2C CH C H + 2H2O

O

Akrolein

OH

O

(H2C)14 C3CH33

KHSO4

A$"* "$" ' #-%-!, &"'% 7-""* $-%& %""* +"*.

"*" &3" 8"!$ +" %""* !*+"# #%"", $"* !*+"# '"

!!*# 2 "'" +" "'" "*" +" "*" #**. $"* &3" #7"% %""*

%%* +"*. !!*# 2 "'" "*" +" "*" & P"$" !*." &"*+"

!!*# 2 "'" +" "*" #**. $"* & S$"*.#"* "$" %""* .%'-%

!!*# 2 "'" %""* +" &3" 8"!$, "*+" %""* * !*."*$*.

.%'-%, $!"*" %""* .%'-% !"#"* #-*-% -' "$" 7-""* *

A$"* "$" "#"$' +"*. !"#"* %"*#- "$" 7-""* * +"*. !!

#-*-% *." P"#' 9*"-% +"*. $.*"#"* *# !*."*. #%&"*

"'"!, H2SO4#" '"." #""%'"- S$"*.#"* *"!"&"* %""* N"O%

$"* H% *.' !*.&$-%'' .%'$" *# !*.&"'%#"* .%'-%

P"$" "#"$' &"*+" !!*# 1 "'" +" & #& H"% *

!*"*$"#"* "$" !*+"# #%"" $"* !*+"# '" !*."*$*. .%'-%,

'$"*.#"* "$" %%* $"# !*."*$*. .%'-% A$"* "$" !*."

'*"*+" 3." !*."*$*. .%'-%, *"!* "&"* +"*. 3"$ $"# ''"

$*."* - H"% * $'"#"* -%& "" #'"%"&"* $"%"! "##! '

#"*. '&*+" "*. "#' $"* ' $" %""* %"* '&*.."

!*+"#"* #'"%"&"* &"'%

T"% 2 T' K-%-!


11/18

-*-& >"'" W"*"

G%'-% 2 "'" H3" 8"!$

M*+"# #%"" 2 "'" H3" 8"!$

L%* 2 "'" P&

M*+"# S" 2 "'" H3" 8"!$

M*." 2 "'" K**. &

A#"$' 1 "'" P& #&

A$"* "#' "$" ' #-%-! +":

a. L+l+!

H2C (CH2)14 C OCH2 (H2C)14 CH3 +

O

N"O:%

). Gl+#er(lH2C OH

HC OH

H2C OH

+ 3NaOCl

H2C ONa

HC ONa

H2C ONa

+ 3HOCl

HCl p

H2C OH

HC OH

H2C OH

+ 3NaClH2SO4

H2C O

HC O

H2C O

O H+ 3H2O

3

' Me!te"a


12/18

H2C O C (CH2)14CH3

H2C O C (CH2)14CH3

HC O C (CH2)14CH3

O

O

O + 3NaOCl

H2C ONa

HC ONa

H2C ONa

OCl

O

(H2C)14 C3CH3+

HCl p

H2C OH

HC OH

H2C OH

+ 3NaClH2SO4

H2C O

HC O

H2C O

O H+ 3H2O

3

%. M+!3a Kela,a Sa4+t

HCl p

H2C OH

HC OH

H2C OH

+ 3NaClH2SO4

H2C O

HC O

H2C O

O H+ 3H2O

3

H2C O C (CH2)16CH2

H2C O C (CH2)16CH2

HC O C (CH2)16CH2

O

O

O+ 3NaOCl

H2C ONa

HC ONa

H2C ONa

+ C8H15 C OCl3C9H18

O

e. M+!3a Kela,a

H2C O C (CH2)16CH3

H2C O C (CH2)16CH3

HC O C (CH2)16CH3

O

O

O+

H2C ONa

HC ONa

H2C ONa

+ C8H15 C OCl

O

H2C OH

HC OH

H2C OH

+H2SO4

H2C O

HC O

H2C O

O H

+3 3 H2O

3 C9H183 NaOCl + HCl

%. A$a%e# A+r6

H2: O C

O

(:H2)1G:H=

H2

: O

H2: O

C

O

(:H2)1G

:H=

C

O

(:H2)1G:H=


13/18

BAB V

PENUTUP

5.1. Ke#+-,$la!

A$"* #'!%"* $" 7-""* * "$"%"& "$" ' '"!% +"*.

!*."*$*. .%'-% $"'"#"* ' "#-%* +" !*.", !*+"# #%"" $"*

!*+"# '" +"*. $"*$" $*."* "$"*+" " *.# S$"*.#"* '"!%

!*."*$*. .%'-% $"'"#"* ' #-%--! "$"%"& !*+"# #%"", !*+"#

'", $"* " (%%*) +"*. $"*$" $*."* "$"*+" "&"* "*" !*3"$

&3" 8"!$

5.2 Sara!

S"#*+" *"*."' " $ #"* #"$" !"'*.9!"'*. #%-!-#


14/18

DAFTAR PUSTAKA

"!%%, N A, B R7, $"* L A U+, 200MIPA U*'"'H"'"*$$*, M"#"''"

P-$3"$3, A, 1664,Bio"imia #asar, UI P'', "#""

T!*"&, 2006, E# A'"! L!"# *& $"* A'"! L!"# T"# *& T"*'

T&"$" K'&""*,$urnal %esehatan, V(l(16)


15/18

LEMBAR PENGESAHAN


16/18

M"#"''", 4 D'! 2015

A#+#te! Prat+a!

(HIKMAWATI ) (NUR QALBI N)


17/18

LAMPIRAN I

Ba"a! Ker&a

1 T' A#-%*

9 D!"'##"* # $"%"! "*. "#' +"*. $"

9 D"!""* KHSO4

9 D"*"'#"* $ ""' " #7%

9 D"!" "*+"

2 T' K-%-!

9 D!"'##"* # $"%"! "*. "#' +"*. $"

9 D"!""* N"O% 2;

9 S%"& 29= !*, $"!""* H% #"

9 D$$"* '%"!" 1 !*

9 D"!""* 0,2 !L 9*"-%

9 D"!""* H2 SO 4 #"

9 D"!" "*" +"*. *#

M K%""

H"'%

M S" L%*M*." A#"$'G%'-%

M K%""

H"'%

M S" L%*M*." A#"$'G%'-%


18/18

LAMPIRAN II

G"!" 1 T' A#-%*

G"!"2 T' K-%-!

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