PERCOBAAN PRAKTIKUM BAKTERI

8/9/2019 PERCOBAAN PRAKTIKUM BAKTERI

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The Experiment about Monera’s Kingdom …

CREATED BY

AGUS DIAN PRATAMA > 03

AISYAH ROHMANIYAH > 04

GITAVANI SILFIYAH > 14

JODY SETIAWAN > 22

SMA NEGERI 1 GRESIK

TAHUN PELAJARAN 2007 - 2008



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MONERA’S KINGDOM

Basic Competence

3.5 To description about the characteristic of Monera’s Kingdom

and to communication the role of Monera’s Kingdom in the

live.

OPENING

Items

Monera’s Kingdom is organism’s member of prokaryotic, that

are organism which don’t have membrane of nucleus cell. In general,

this organism have unicellular characteristic with nucleus item which

dispersed inside protoplasm cell.

The organisms which include the member of Monera are

bacteria and Cyanophyta ( Green-Blue algae ). The main different of

them is the ability on photosynthesis. Cyanophyta can for

photosynthesis, while of bacteria, in general can’t for

photosynthesis, except several kinds of bacteria, not all.

Green-Blue algae is kind of Monera’s Kingdom groups because

Green-Blue algae have prokaryotic characteristic, one cell, the

others have many cells with thread’s form and colony’s form, have

chlorophyll. Have fikobilin’s pigment ( the bundle of fikoeretrin and

fikosianin ). The reproduction with splitting itself, fermentation, or

heterocyst.



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THE FIRST ( 1 st ) EXPERIMENT

Purpose

Perceiving bacterium forms which there in the rotten food-

stuff.

Materials and Appliances

• The Materials

1. The rotten food

a. Rotten tomato ( in 3 days rotten )

b. Rotten Potato ( in 3 days rotten )

c. Rotten of Coconut’s water ( in 2 days rotten )

2. Blue’s Metilen

• The Appliances

1. Microscope 5. Desk mop

2. Cover slip 6. Thick clothes mop

3. Glass side 7. Pipette

4. Toothpick

Steps or Procedure

1. Prepare the glass side, cover slip and the microscope.

2. Cleaning the glass side with sterile’s mop until clean.



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3. Take the thick water ( lendir ) of rotten’s food with toothpick, oily or

spread on the glass side as flimsy as possible.

4. Give blue’s metilen on the top of object ( the thick water on glass

side ) and covering them with cover slip.

5. Take the glass side ( with object ) on the stage for observation.

6. Perceiving by microscope with low magnification, then changes it

into strong magnification for clear result.

7. Arrange the coarse adjustment knob and fine adjustment knob for

clear result.

8. Perceiving the form and draw it.

9. Answer the questions relation with that form.



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THE SECOND ( 2 nd ) EXPERIMENT

Purpose

Perceiving bacterium forms which there in the crust’s water

( basic’s water from aquarium ).

Materials and Appliances

• The Material

1. The crust’s water ( basic’s water from aquarium )

• The Appliances

1. Microscope 5. Desk mop

2. Cover slip 6. Thick clothes mop

3. Glass side 7. Pipette

4. Toothpick 8. Water

Steps or Procedure

1. Prepare the glass side, cover slip and the microscope.

2. Cleaning the glass side with sterile’s mop until clean.

3. Take the crust’s water from basic’s aquarium with toothpick, oily or

spread on the glass side as flimsy as possible.

4. Give water on the top of object ( the crust’s water on glass side ) and

covering them with cover slip.

5. Take the glass side ( with object ) on the stage for observation.



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6. Perceiving by microscope with low magnification, then changes it

into strong magnification for clear result.

7. Arrange the coarse adjustment knob and fine adjustment knob for

clear result.

8. Perceiving the form and draw it.

9. Answer the questions relation with that form.



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THE RESULT FROM THE FIRST ( 1 st ) EXPERIMENT

In the first’s experiment, we get some picture from any object which

we perceiving with microscope.

• FROM ROTTEN TOMATO

Magnification :

* Ocular’s lens = 12,5

* Objective lens= 10

• FROM ROTTEN POTATO

Magnification :



12,5 x 10= 125 kali12,5 x 10= 125 kali

12,5 x 10= 125 kali



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• FROM ROTTEN OF COCONUT’S WATER

Magnification :



12,5 x 10= 125 kali



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THE RESULT FROM THE SECOND ( 2 nd )EXPERIMENT

In the second’s experiment, we get some picture and event from any

object which we perceiving with microscope.

The Result of Observation

Magnification :


* Objective lens = 10

12,5 x 10= 125 kali



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Question

1. How the form of the algae which you found ?

The form of algae is Oscillatoria’s form

2. How the characteristic ?

a. Active for moving

b. Have purple’s color in the surface’s skin

c. Have blue’s color inside of the bacteria

d. Oscillatoria’s form

e. Long and dull



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CLOSING

Bacterium, of Latin word of bacterium ( plural, bacteria), is giantgroup of life organism. They very small ( microscopic) and most unicellular

have single cell, with cellular structure which relative modestly without

nucleus / core of cell, cytoskeleton, and other organelle like and

mitochondria of chloroplast. Their cellular structure is explained

furthermore in article concerning prokaryote, because bacterium represent

prokaryotes, to differentiate them with organism owning cell more

complex, referred

as by eukaryote.

Term " bacterium"

has been applied by

for all prokaryote or

for big group of

them, depends on

idea concerning

their [relation/link].

Bacteria have many

forms. We get some

form from the

experiment using

microscope.

The Crust’s water form basic aquarium has Oscillatoria’s form.

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