Hasil Isolasi total genom Hasil amplifikasi 16 sRNA

Perhitungan konsentrasi DNA dengan nanodrop (50,89 ng/ul)

Hasil Sequencing 16S rRNA- 63 Forward

NNNNNNNNGNCNNNNTNCNGCGGGCTGGCTCCTAAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGT

GGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTC

CAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGT

TTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCC

CCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCAACTAAGATCAAGGGTTG

CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTCTGCCCC

CGAAGGGGACGTCCTATCTCTAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTA

AACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCG

GAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTG

GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAGAGTCGCCT

TCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCNTCTGCACTCAAG

TTCCCCAGTTTCCAATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAACCGCCTGCGAGCCC

TTTACGCCCATAATTCCGGACNACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTT

TCTGGTTAGNACCGTCAAGGNACCGCCCTATCGAACGGNACTGTCTNCCTACAACGANNTTACGATCCGGAAAAN

NNNTCACTTCNNCGCNTNNTNCGTCGACTTNGTCATGCGANATCNNNNNCTGCNTCCGANGANCNGGNCCNNN

NNNNNNCCANNGNGNCCGANCACCCTNNTCCAG

Hasil Sequencing 16S rRNA - 1387 Reverse

NNNNNNNNNNNGGNNNNNTATAATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGA

CGGGTGAGTAACACGTGNGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGG

TTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCT

AGTTGGTGAGGTAACGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACT

GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAA

CGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGG

CGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCA

AGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCA

ACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGA

AATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAA

GCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAGGGGGTT

TCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAGG

AATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTT

GACATCCNCTGACAATCCTAGAGATAGGACGTCCCCTTTCGGGGCAGANNGACNGGNGTTGCANGNTGTCCGN

Data Konsensus

NNNNNNNNGNCNNNNTNCNGCGGGCTGGCTCCTAAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGT

GGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTC

CAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGT

TTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCC

CCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCCCAACTGAATGCTGGCAACTAAGATCAAGGGTTG

CGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGRMMACCATGCAMCACCTGTCACTCTGCCC

CSRAAGGGGACGTCCTATCTCTAGGATTGTCAGAGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATT

AAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGC

GGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGT

GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAGAGTCGC

CTTCGCCACTGGTGTTCCTCCACATCTCTACGCATTTCACCGCTACACGTGGAATTCCACTCTCCTCTTCTGCACTCA

AGTTCCCCAGTTTCCAATGACCCTCCCCGGTTGAGCCGGGGGCTTTCACATCAGACTTAAGAAACCGCCTGCGAGC

CCTTTACGCCCAATAATTCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGG

CTTTCTGGTTAGGTACCGTCAAGGTACCGCCCTATTCGAACGGTACTTGTTCTTCCCTAACAACAGAGCTTTACGAT

CCGRAAAMCTTCATCACTYMCGCGGCGTTGCTCCGTCAGACTTTCGTCCATTGCGGAAGATTCCCTACTGCTGCCT

CCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCNTCMRGTCGGCTACGCATCGTCGCC

TTGGTGAGCCGTTACCTCACCAACTAGCTAATGCGCCGCGGGTCCATCTGTAAGTGGTAGCCGAAGCCACCTTTTA

TGTTTGAACCATGCGGTTCAAACAACCATCCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAGTCTTACAGGCAG

GTTACNCACGTGTTACTCACCCGTCCGCCGCTAACATCAGGGAGCAAGCTCCCATCTGTCCGCTCGACTGCATTATA

NNNNNCCNNNNNNNNNNN

Hasil kromatogram 63F

Hasil kromatogram 1387R

Hasil analisis BLAST-N

Data yang diperoleh menunjukan semua sampel memiliki tingkat kesesuaian/ homologi yang tinggi

yaitu 97% (Query Coverage) dan 99% (Max Ident) dengan bakteri Bacillus subtilis pada GeneBank

Hasil pewarnaan gram (1000x) Hasil pewarnaan spora (1000x)

Penghambatan bakteriosin hasil pengendapan ammonium sulfat terhadap Vibrio Harvey

Ket : 1:1 ( Vibrio harvey 100 ul : bakteriosin 100 ul)

1:2 ( Vibrio harvey 100 ul : bakteriosin 200 ul)

Pengendapan 70 %

Pengenceran Jumlah koloni sel/ml Persen penghambatan (%)

Kontrol

10^4 TBUD

10^5 TBUD

10^6 187

10^7 130 13 X 109

1:1

10^4 340

10^5 260

10^6 123

10^7 97 9,7 x 109 25,38 %

1:2

10^4 TBUD

10^5 143

10^6 97

10^7 66 6,6 x 109 49,23 %

Pengendapan 80 %

Pengenceran Jumlah koloni sel/ml Persen penghambatan (%)

Kontrol

10^4 TBUD

10^5 TBUD

10^6 187

10^7 130 13 X 109

1:1

10^4 TBUD

10^5 TBUD

10^6 176

10^7 102 10,2 x 109 21,54

1:2

10^4 176

10^5 198

10^6 97

10^7 78 7,8 x 109 40,00