DAFTAR ISI Halaman

Daftar Isi ........................................................................................................... iDaftar Gambar.................................................................................................. iii Daftar Tabel...................................................................................................... iv

BAB I PENDAHULUAN1.1. Latar Belakang................................................................................. 11.2. Rumusan Masalah............................................................................ 21.3. Tujuan .............................................................................................. 21.4. Manfaat............................................................................................. 2

1.1.1. Manfaat Ilmiah......................................................................... 21.1.2. Manfaat Klinis ......................................................................... 2

BAB II TINJAUAN PUSTAKA2.1 Demam Berdarah Dengue (DBD)..................................................... 3

2.1.1 Definisi DBD............................................................................ 32.1.2 Epidemiologi............................................................................ 32.1.3 Faktor Penyebab DBD............................................................ 42.1.4 Imunopatogenesis................................................................... 62.1.5 Patofisiologi............................................................................. 132.1.6 Diagnosis................................................................................. 182.1.7 Penatalaksanaan..................................................................... 20

2.2 Saliva................................................................................................ 252.3 Imunoglobulin A................................................................................ 27

2.3.1 Produksi Ig A pada saliva........................................................ 272.3.2 Produksi Ig A pada Pasien DHF.............................................. 312.3.4 Produksi Ig A Secretory pada pasien DHF (Mucosal Homing) 32

2.4 Produksi Antibodi Poliklonal Anti-Immunoglobulin A pada Kelinci yang Diimunisasi Secretory Immunoglobulin A (sIgA)..................... 33

2.5 Strategi Pencegahan ....................................................................... 332.5.1 Pencegahan di level komunitas .............................................. 352.5.2 Pencegahan di level individu................................................... 37..........................................................................................................

BAB III RANCANGAN METODE PENDEKATAN3.1. Metode Konseptual ......................................................................... 403.2. Rancangan Pelaksanaan................................................................. 40

3.2.1 Pengambilan Sampel Saliva.................................................. 413.2.2 Metode Isolasi Protein dari Sampel Saliva............................. 413.2.3 Metode Elektroforesis Sampel Saliva.................................... 413.2.4 Metode Elektroelusi dan Dialisa............................................. 413.2.5 Metode pembuatan Anti Antibodi sIgA dengan Metode

Poliklonal................................................................................ 413.2.6 Metode Purifikasi Anti – Antibodi sIgA................................... 423.2.7 Metode Uji Spesifisitas Anti - Antibodi dengan teknik Dot

Blot......................................................................................... 423.2.8 Metode Pembuatan Kit Dipstick dengan Kandungan Anti –

Antibodi sIgA.......................................................................... 423.2.9 Metode Pengujian Kit Dipstick pada pasien anak dengan

DBD........................................................................................ 42

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BAB IV KESIMPULAN DAN SARAN4.1 Kesimpulan ....................................................................................... 444.2 Saran ................................................................................................ 44

DAFTAR PUSTAKA ........................................................................................ 45

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DAFTAR GAMBAR

Gambar 2.1 Kompleks ImunTidak Infeksius..................................................... 8

Gambar 2.2 Kompleks Imun Infeksius.............................................................. 8

Gambar 2.3 Kompleks Virus Tidak Dapat Dineutralisasi.................................. 9

Gambar 2.4 Patofisiolgi Demam Berdarah Dengue (DBD).............................. 10

Gambar 2.5 Limfosit T...................................................................................... 11

Gambar 2.6 Patogenesis Demam Berdarah Dengue dan Sindrom Syok

Dengue............................................................................................... 12

Gambar 2.7 Respon Imun Infeksi Virus Dengue.............................................. 15

Gambar 2.8 Mekanisme Perembesan Plasma Akibat Infeksi Virus Dengue.... 18

Gambar 2.9 Penanganan Tersangka DBD Tanpa Syok................................... 21

Gambar 2.10 Pemberian Cairan Pada tersangka DBD Dewasa di Ruang

Rawat................................................................................................. 22

Gambar 2.11 Penatalaksanaan DBD dengan Peningkatan Hematokrit >20%. 22

Gambar 2.12 Tatalaksana Sidrom Syok Dengue Pada Dewasa...................... 23

Gambar 2.13 Switching Isotipe Ig..................................................................... 28

Gambar 2.14 Human Immunoglobulin A dan secretory Immunoglobulin A...... 29

Gambar 2.15 Mucosal Homing......................................................................... 33

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DAFTAR TABEL

Tabel 2.1 Klasifikasi Derajat keparahan DBD .................................................. 19

Tabel 2.2 Perbedaan IgA serum dan sIgA ....................................................... 30

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