Post on 01-Feb-2018
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PertanyaanPertanyaan Apakah tujuan dilakukannya prinsip-prnsip biosafety
dalam bekerja di lab mikrobiologi? Apa saja bahan-bahan yang termasuk dalam kategori
bahan biologi berbahaya? Berdasarkan tingkat keamanan kerja di lab
mikrobiologi, biosafety lab dibedakan menjadi berapa level? Sebutkan !
Apakah level biosafety yang sesuai untuk mengerjakan jenis-jenis bakteri berikut ini: Clostridium botulinum E.coli Mycobacterium tuberculosis
Apakah tujuan dilakukannya prinsip-prnsip biosafety dalam bekerja di lab mikrobiologi?
Apa saja bahan-bahan yang termasuk dalam kategori bahan biologi berbahaya?
Berdasarkan tingkat keamanan kerja di lab mikrobiologi, biosafety lab dibedakan menjadi berapa level? Sebutkan !
Apakah level biosafety yang sesuai untuk mengerjakan jenis-jenis bakteri berikut ini: Clostridium botulinum E.coli Mycobacterium tuberculosis
Hady Anshory T, M.Sc.,AptHady Anshory T, M.Sc.,Apt
Prinsip-prinsip Biosafety LaboratoryPrinsip-prinsip Biosafety Laboratory
Lambang biosafetyLambang biosafety
Safety from exposure to Infectious Agents
Safety from exposure to Infectious Agents
Smallpox
Bagaimana bekerja di Lab agar terhindar dari agen-
agen infeksius yang membahayakan
Lab biosafety
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To protect: Workers/Students Products/Experimental results Environment/Laboratory classroom
To protect: Workers/Students Products/Experimental results Environment/Laboratory classroom
Kenapa harus biosafety ??Kenapa harus biosafety ??
Viruses Bacteria Fungi Prions Recombinant DNA Human and Primate Cells, Tissues, and Body
Fluids Transgenic Plants, Animals and Insects
Viruses Bacteria Fungi Prions Recombinant DNA Human and Primate Cells, Tissues, and Body
Fluids Transgenic Plants, Animals and Insects
Materi biologi berbahaya (Biohazards)Materi biologi berbahaya (Biohazards)
Transgenic Plants, Animals and Insects Transgenic Plants, Animals and Insects
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Sebagai pedoman untuk bekerja dengan aman di laboratorium mikrobiologi dan bimemedis sehingga dapat mengurangi resiko terkontaminasi oleh material biologis berbahaya
Sebagai pedoman untuk bekerja dengan aman di laboratorium mikrobiologi dan bimemedis sehingga dapat mengurangi resiko terkontaminasi oleh material biologis berbahaya
Prinsip-prinsip biosafetyPrinsip-prinsip biosafety
Biosafety levels (BSL)1-4
Sifat bahan yang ditangani pada BSL-1:
Karakteristik baik Bahan yang tidak menimbulkan penyakit pada
manusia Bahan yang memiliki efek bahaya yang rendah
terhadap personil lab atau lingkungan.
Sifat bahan yang ditangani pada BSL-1:
Karakteristik baik Bahan yang tidak menimbulkan penyakit pada
manusia Bahan yang memiliki efek bahaya yang rendah
terhadap personil lab atau lingkungan.
Biosafety level 1 (BSL-1)Biosafety level 1 (BSL-1)
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Biosafety level 1 (BSL-1)Biosafety level 1 (BSL-1)
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
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Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Biosafety level 1 (BSL-1)Biosafety level 1 (BSL-1)
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
are needed to see this picture.
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Biosafety level 1 (BSL-1)Biosafety level 1 (BSL-1)
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Contoh biohazards BSL-1 :
E. coli Saccharomyces cerevisiae Transgenic Plants Plasmids Fungi Mold Yeast
Biosafety level 1 (BSL-1)Biosafety level 1 (BSL-1)
Personal Protective Equipment (PPE)
Lab coat or apron Safety glasses or goggles Gloves as needed
Personal Protective Equipment (PPE)
Lab coat or apron Safety glasses or goggles Gloves as needed
Perlengkapan keamanan BSL-1Perlengkapan keamanan BSL-1
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Basic lab untuk BSL 1 dan 2
Sifat bahan yang ditangani pada BSL-2 :
Bahan yang berpotensi menimbulkan bahaya pada personil lab atau lingkungan
Sifat bahan yang ditangani pada BSL-2 :
Bahan yang berpotensi menimbulkan bahaya pada personil lab atau lingkungan
Biosafety level 2 (BSL-2)Biosafety level 2 (BSL-2)
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Biosafety level 2 (BSL-2)Biosafety level 2 (BSL-2)
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Biosafety level 2 (BSL-2)Biosafety level 2 (BSL-2)
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Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Biosafety level 2 (BSL-2)Biosafety level 2 (BSL-2)
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Contoh biohazard BSL-2:
Human blood or body fluids E. coli 0157:H7 Clostridium botulinum Human cells in cell culture Herpes Simplex Virus Patient specimens
Biosafety level 2 (BSL-2)Biosafety level 2 (BSL-2)
Personal Protective Equipment (PPE) Lab coat or apron Safety glasses or goggles Gloves Biosafety cabinet
Aerosols or splashes Large volumes High concentrations
Personal Protective Equipment (PPE) Lab coat or apron Safety glasses or goggles Gloves Biosafety cabinet
Aerosols or splashes Large volumes High concentrations
Perlegkapan keamanan BSL-2Perlegkapan keamanan BSL-2 Desain lab BSL-2Desain lab BSL-2
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Sifat bahan pada BSL-3
Bahan asing Menyebar lewat udara Menimbulkan penyakit/efek yang serius Teknik pengendalian penyakit dari bahan
tersedia atau tidak
Sifat bahan pada BSL-3
Bahan asing Menyebar lewat udara Menimbulkan penyakit/efek yang serius Teknik pengendalian penyakit dari bahan
tersedia atau tidak
Biosafety level 3 (BSL-3)Biosafety level 3 (BSL-3)
Human Immunodeficiency Virus Mycobacterium tuberculosis Coxiella burnetii
Human Immunodeficiency Virus Mycobacterium tuberculosis Coxiella burnetii
Contoh bahan berbahaya BSL-3Contoh bahan berbahaya BSL-3
Public access NOT permitted Autoclave required and waste is
disposed at the end of day Required foot activated handwashing
sink and controls No sharps unless absolutely necessary Aerosol minimization procedures
required
Public access NOT permitted Autoclave required and waste is
disposed at the end of day Required foot activated handwashing
sink and controls No sharps unless absolutely necessary Aerosol minimization procedures
required
Desain lab untuk BSL-3
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Biosafety Level-4 Maximum containment facilities Pressurized Containment Suite
BSL-3 + Class III Biosafety Cabinet Chemical decontamination showers Liquid effluent collection / decontamination
Biosafety Level-4 Maximum containment facilities Pressurized Containment Suite
BSL-3 + Class III Biosafety Cabinet Chemical decontamination showers Liquid effluent collection / decontamination
Lassa Fever Virus Ebola Hemmorrhagic
Fever Virus Marburg Virus Herpes B Virus
Lassa Fever Virus Ebola Hemmorrhagic
Fever Virus Marburg Virus Herpes B Virus
Desain lab untuk BSL-4
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Tehnik dasar yang harus dikuasai ???Tehnik dasar yang harus dikuasai ???
1. Memahami aturan lab2. Mengetahui teknik dekontaminasi3. Mengetahui teknik P3K di laboraorium
1. Memahami aturan lab2. Mengetahui teknik dekontaminasi3. Mengetahui teknik P3K di laboraorium
No Smoking, Eating, Applying cosmetics, lip balm, contacts
Wash hands before and after proceduresDecontaminate lab bench before and after work
No Smoking, Eating, Applying cosmetics, lip balm, contacts
Wash hands before and after proceduresDecontaminate lab bench before and after work
Peraturan Dasar Lab
Sterilization: destroy all microbial life, including spores Disinfection: destroy a majority of microbial life, but not
necessarily spores Antiseptic: germistat used on skin to inhibit growth of
microorganisms
Sterilization: destroy all microbial life, including spores Disinfection: destroy a majority of microbial life, but not
necessarily spores Antiseptic: germistat used on skin to inhibit growth of
microorganisms
Teknik Dekontaminasi
Methods Heat: steam heat, dry heat, incineration Chemical: bleach, ethanol, hydrogen peroxide,
ethylene oxide, paraformaldehyde Radiation
Methods Heat: steam heat, dry heat, incineration Chemical: bleach, ethanol, hydrogen peroxide,
ethylene oxide, paraformaldehyde Radiation
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Awas ada tumpahan bahan biologi..!!
1. Evakuasi tempat, personil dan area dipagari/dibatasi
2. Gunakan pelindung (PPE); 3. Tutup area tumpahan dengan lap dan
tuangi menggunakan kaporit/desinfektan
4. Biarkan selama 12-20 menit.5. Besihkan area tersebut6. Bila ada pecahan kaca, ambil
menggnakan penjepit7. Buang pada tempat yang disediakan
(biohazard)
Cara mengatasi..,
Splash to Eye or Needlestick Injury Rinse thoroughly for 15 minutes at the eyewash or
sink Call medical center
Splash to Eye or Needlestick Injury Rinse thoroughly for 15 minutes at the eyewash or
sink Call medical center
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Tahanks !
UCSD Biosafety: http://blink.ucsd.edu/safety/research-lab/biosafety/
Biological Safety MSDS: http://www.phac-aspc.gc.ca/msds-ftss/index-eng.php
NIH BMBL:http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm
See Handout for additional resources..
UCSD Biosafety: http://blink.ucsd.edu/safety/research-lab/biosafety/
Biological Safety MSDS: http://www.phac-aspc.gc.ca/msds-ftss/index-eng.php
NIH BMBL:http://www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm
See Handout for additional resources..