Bioteknologi Pertanian
Lisnawita
Mikroba
Rekombinan
Mikroba rekombinan
DNA rekombinan
Metode perakitan
DNA (Deoxyribonucleic acid)
adalah sejenis asam nukleat yang tergolong biomolekul utama penyusun berat kering setiap organisme
peran DNA di dalam sebuah sel adalah sebagai materi genetik
DNA adalah pembawa informasi genetik di dalam suatu sel
Kumpulan teknik atau motode yang
digunakan untuk mengkombinasikan
gen-gen secara buatan
Proses rekombinasi
Hasil rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Mikroba
Rekombinan
Mikroba rekombinan
DNA rekombinan
Metode perakitan
DNA (Deoxyribonucleic acid)
adalah sejenis asam nukleat yang tergolong biomolekul utama penyusun berat kering setiap organisme
peran DNA di dalam sebuah sel adalah sebagai materi genetik
DNA adalah pembawa informasi genetik di dalam suatu sel
Kumpulan teknik atau motode yang
digunakan untuk mengkombinasikan
gen-gen secara buatan
Proses rekombinasi
Hasil rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Mikroba rekombinan
DNA rekombinan
Metode perakitan
DNA (Deoxyribonucleic acid)
adalah sejenis asam nukleat yang tergolong biomolekul utama penyusun berat kering setiap organisme
peran DNA di dalam sebuah sel adalah sebagai materi genetik
DNA adalah pembawa informasi genetik di dalam suatu sel
Kumpulan teknik atau motode yang
digunakan untuk mengkombinasikan
gen-gen secara buatan
Proses rekombinasi
Hasil rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
DNA (Deoxyribonucleic acid)
adalah sejenis asam nukleat yang tergolong biomolekul utama penyusun berat kering setiap organisme
peran DNA di dalam sebuah sel adalah sebagai materi genetik
DNA adalah pembawa informasi genetik di dalam suatu sel
Kumpulan teknik atau motode yang
digunakan untuk mengkombinasikan
gen-gen secara buatan
Proses rekombinasi
Hasil rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Kumpulan teknik atau motode yang
digunakan untuk mengkombinasikan
gen-gen secara buatan
Proses rekombinasi
Hasil rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Rekombinasi antara molekul DNA dari organisme hidup yang berbeda fenomena umum di alam
Contoh Corynebacterium diphtheriae yang diinfeksi oleh virus szlig menghasilkan toksin yang bertanggung jawab terhadap gejala dipteri
Perubahan genetik pada bakteri yang disebabkan virus merupakan rekayasa genetik di alam
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Duplikasi fenomena alam di laboratorium
Mengembangkan metode introduksi berbagai
informasi genetik ke dalam suatu organisme
Produksi bahan mahal yang tidak mungkin
dibuat secara tradisional
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
PLASMID
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Kromoson ndash vs - Plasmid
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Memotong DNA
Menggunakan enzim restriksi yaitu
enzim yang memotong dsDNA (double
stranded DNA) pada situs spesifik
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Menyambung DNA
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Tahapan DNA rekombinan
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Teknologi DNA rekombinan
(Kloning gen)
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Kloning DNA ke plasmid
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Video biotek Steps in Cloning a Gene -
YouTube [360p]mp4
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Video biotekRecombinant DNA copy2004
Demonstratives Inc - YouTube [360p]mp4
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Seleksi bakteri pembawa DNA rekombinan
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Performing the Spread Plate method I
1 Choose appropriate nutrient agar plate with the correct antibiotic (and X-gal) if visual screening
2 Using sterile technique transfer a loopful of bacteria from a culture tube onto plate (or 100l of bacterial culture using a pipette)
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
3 Dip glass ldquohockey stickrdquo in 70 ethanol Holding it DOWNWARDS flame until alcohol is burned off DO NOT put back into alcohol
4 Remove lid of petri dish With one hand rotate dish With other hand move hockey stick lightly over surface to spread the inoculum evenly
Performing the Spread Plate method II
70 EtOH
ldquoSpreaderrdquo or ldquoHockey Stickrdquo
Keep flame away from alcohol
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Performing a Plate Streak I
1 Flame metal inoculating loop let cool momentarily
2-3 Using sterile technique transfer a loop of bacterial culture or single colony onto loop
4 With one hand remove lid of dish With other hand lightly brush the loop back and forth on one quadrant of the dish
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Performing a Plate Streak II
4 Reflame metal inoculating loop let cool momentarily
567 Rotate petri dish 90deg Use 1st streak as inoculum for 2nd streak (only pass the loop through the 1st streak once) Repeat once more rotating dish 90deg and sterilizing loop again
8 Incubate on at 37degC
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Plate Streak Method
This is an example of a good streak for isolation using the four corners method
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
This is not a great streak plate but it is serviceable as there are a few isolated colonies - would have been better if the loop had been flamed between each sector
This is an example of how NOT to streak for isolation Scribbling is not streaking and most likely will not result in isolated colonies
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
After Incubating the plate overnight at 37degC- individual
colonies of transformed bacteria
should be seen
Each team will pick two individual colonies
(clones) and streak on a new plate (single
colony purification) for next week
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Bacterial colonies transformed with pUC18
blue colonies (contain non-recombinant DNA molecules)
White colonies (contain recombinant DNA molecules)
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Video biotek dna cloning - YouTube
[360p]mp4
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Manfaat teknologi DNA rekombinan
Dengan mengisolasi dan mempelajari masing-
masing gen akan diperoleh pengetahuan
tentang fungsi dan mekanisme kontrolnya
teknologi ini memungkinkan diperolehnya
produk gen tertentu dalam waktu lebih cepat
dan jumlah lebih besar daripada produksi
secara konvensional
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Deteksi keberadaan DNA sisipan
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
1 Pre Denaturasi
2 Denaturasi
3 Annealing
4 Ekstensi
5 Post ekstensi (pemantapan)
Tahapan-tahapan PCR
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
PCR Animation Please click here
1st cycle 2nd cycle 3rd cycle
Process
Denature
Anneal Primer
Replicate
DNA
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
1 2 5 4 M 8
10 11 6 7
391 bp
M M M 7 6 5 4
A B
3
238 bp
9
Contoh hasil PCR
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Hasil penentuan urutan nukleotida DNA
sisipan (DNA sekuensing)
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
GR1-Jatim 2 TTGTTGTACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR3-Jatim 3 TTGTTGCACGTGCCGTACCTTGCGGCATGTCTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR2-Jatim 1 TTGTTGTACGTGCCGTACCTTGCGGCATGTTTGCGCTTGTGTGCTACGTCCGTGGCCGTG 60
GR1-Jatim 2 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR3-Jatim 3 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR2-Jatim 1 ATGAGACGACGTGTTAGGACCCGTGCCTGGCATTGGCACGTGGTTTAAGACTTGATGAGT 120
GR1-Jatim 2 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR3-Jatim 3 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR2-Jatim 1 GCCCGCAGGCACCGCCAGCTTTTTCCCATTTTTATTTATTTTTTATGCAATTCGATTGCT 180
GR1-Jatim 2 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGTGGATCGATGAAGATCGCTAGCC 240
GR3-Jatim 3 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCSKGGATCGATGAAGATCGCTAGCC 240
GR2-Jatim 1 AAAATATTCTAGTCTTATCGGTGGATCACTCGGCTCGKGGATCGATGAARATCGCTAGCC 240
GR1-Jatim 2 TTCTGCA 247
GR3-Jatim 3 TTCTGCA 247
GR2-Jatim 1 TTCTGCA 247
Hasil pembacaan DNA sekuensing
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
Bidang Hukum Pelaku kejahatan dapat diidentifikasi dengan menggunakan analisis Sidik jari DNA
Misalnya Kasus perkosaan
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
02 Hybrid Seed
14 Food
18 Starch
37 Alcohol
58 Sweeteners
447 Animal FeedResidual
168 Exports
256 Ending Stocks (Buffer against a bad crop)
Avg Annual US Usage
Oil is extracted from the germ (embryo) for cooking Starch in building materials
or intravenous solutions the shell (hull) is used in animal
feed
Source National Geographic June 1993 p91-117
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