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14
IOP Conference Series: Earth and Environmental Science PAPER • OPEN ACCESS Preface To cite this article: 2020 IOP Conf. Ser.: Earth Environ. Sci. 519 011001 View the article online for updates and enhancements. This content was downloaded from IP address 103.119.54.215 on 06/10/2020 at 03:11

Transcript of Preface - UNUSA Repository

IOP Conference Series Earth and Environmental Science

PAPER bull OPEN ACCESS

PrefaceTo cite this article 2020 IOP Conf Ser Earth Environ Sci 519 011001

View the article online for updates and enhancements

This content was downloaded from IP address 10311954215 on 06102020 at 0311

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 011001

IOP Publishing

doi1010881755-13155191011001

1

PREFACE

The 1st International Conference Earth Science and Energy (Formerly ICEMINE) was successfully

held on 7-8th November 2019 in Kuala Lumpur Malaysia The conference is organized in collaboration

between Universitas Muhammadiyah Sidoarjo Sidoarjo (Indonesia) and Kresna Nusantara (PT

Kresna Acitya Nusantara Mediatama Indonesia) The conference also in cooperation and supported

by Relawan Jurnal Indonesia (Indonesia)

ICESE 2019 welcomes intellectuals worldwide to join and share their ideas and research findings on

issues concerning all fields of Earth Science and Energy Therefore in the end of full paper submission

deadline there are 131 submitted papers with 58 accepted papers for publication in the conference

proceedings All papers submitted were processed through a normative procedure for the reason of

quality assurance They were firstly reviewed by the content reviewers Then they were sent to the

language reviewers and finally to the template reviewers In brief three teams of reviewers have

reviewed the selected papers to reach publication In general this conference has gained direction

and supervision from the International Advisory Board and International Scientific Committee to

ensure that the whole process meets the internationally accepted academic standards This

conference has also increasingly showed its prestige with the presence of highly distinguished

speakers Ahmad Fudloli PhD from Universiti Kebangsaan Malaysia (Malaysia) Dr Prantasi Harni

Tjahjanti from Universitas Muhammadiyah Sidoarjo (Indonesia) and Andino Maseleno Ph D from

Universitas Tenaga Nasional (Malaysia) who delivered their insightful presentations

On behalf of the Organizing Committee we sincerely express our gratitude and appreciation to all

those who have involved in enabling this conference achieving the desired aim or result officials

resource persons delegates and guests all publication partners and sponsoring agencies our

heartfelt gratitude goes to honorable speakers and presenters who have shared knowledge and

experiences in developing a robust discussion and transformative intellectual exchange We wish all

activities during this two-day conference will stimulate further relation collaboration and

constructive engagement among those intellectuals in bringing their contributions for the

betterment of the world The Editors

Editors

Ahmad Fudholi

Andino Maseleno

Rahmat Hidayat

Mochammad Tanzil Multazam

Robbi Rahim

Ikhwan Arif

Andista Candra Yusro

Dwi Fajar Saputra

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 011002

IOP Publishing

doi1010881755-13155191011002

1

Peer review statement

All papers published in this volume of IOP Conference Series Earth and Environmental Science have

been peer reviewed through processes administered by the proceedings Editors Reviews were conducted

by expert referees to the professional and scientific standards expected of a proceedings journal published

by IOP Publishing

Table of contents

Open all abstracts

Preface

Agriculture

Biology

Bioprocessing

Volume 519

2020

Previous issue Next issue

International Conference Earth Science amp Energy 7-8 November 2019 Kuala Lumpur Malaysia

Accepted papers received 01 June 2020

Published online 06 July 2020

011001OPEN ACCESS

Preface

View article PDFOpen abstract

011002OPEN ACCESS

Peer review statement

View article PDFOpen abstract

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Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

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  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 011001

IOP Publishing

doi1010881755-13155191011001

1

PREFACE

The 1st International Conference Earth Science and Energy (Formerly ICEMINE) was successfully

held on 7-8th November 2019 in Kuala Lumpur Malaysia The conference is organized in collaboration

between Universitas Muhammadiyah Sidoarjo Sidoarjo (Indonesia) and Kresna Nusantara (PT

Kresna Acitya Nusantara Mediatama Indonesia) The conference also in cooperation and supported

by Relawan Jurnal Indonesia (Indonesia)

ICESE 2019 welcomes intellectuals worldwide to join and share their ideas and research findings on

issues concerning all fields of Earth Science and Energy Therefore in the end of full paper submission

deadline there are 131 submitted papers with 58 accepted papers for publication in the conference

proceedings All papers submitted were processed through a normative procedure for the reason of

quality assurance They were firstly reviewed by the content reviewers Then they were sent to the

language reviewers and finally to the template reviewers In brief three teams of reviewers have

reviewed the selected papers to reach publication In general this conference has gained direction

and supervision from the International Advisory Board and International Scientific Committee to

ensure that the whole process meets the internationally accepted academic standards This

conference has also increasingly showed its prestige with the presence of highly distinguished

speakers Ahmad Fudloli PhD from Universiti Kebangsaan Malaysia (Malaysia) Dr Prantasi Harni

Tjahjanti from Universitas Muhammadiyah Sidoarjo (Indonesia) and Andino Maseleno Ph D from

Universitas Tenaga Nasional (Malaysia) who delivered their insightful presentations

On behalf of the Organizing Committee we sincerely express our gratitude and appreciation to all

those who have involved in enabling this conference achieving the desired aim or result officials

resource persons delegates and guests all publication partners and sponsoring agencies our

heartfelt gratitude goes to honorable speakers and presenters who have shared knowledge and

experiences in developing a robust discussion and transformative intellectual exchange We wish all

activities during this two-day conference will stimulate further relation collaboration and

constructive engagement among those intellectuals in bringing their contributions for the

betterment of the world The Editors

Editors

Ahmad Fudholi

Andino Maseleno

Rahmat Hidayat

Mochammad Tanzil Multazam

Robbi Rahim

Ikhwan Arif

Andista Candra Yusro

Dwi Fajar Saputra

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 011002

IOP Publishing

doi1010881755-13155191011002

1

Peer review statement

All papers published in this volume of IOP Conference Series Earth and Environmental Science have

been peer reviewed through processes administered by the proceedings Editors Reviews were conducted

by expert referees to the professional and scientific standards expected of a proceedings journal published

by IOP Publishing

Table of contents

Open all abstracts

Preface

Agriculture

Biology

Bioprocessing

Volume 519

2020

Previous issue Next issue

International Conference Earth Science amp Energy 7-8 November 2019 Kuala Lumpur Malaysia

Accepted papers received 01 June 2020

Published online 06 July 2020

011001OPEN ACCESS

Preface

View article PDFOpen abstract

011002OPEN ACCESS

Peer review statement

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Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

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  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 011002

IOP Publishing

doi1010881755-13155191011002

1

Peer review statement

All papers published in this volume of IOP Conference Series Earth and Environmental Science have

been peer reviewed through processes administered by the proceedings Editors Reviews were conducted

by expert referees to the professional and scientific standards expected of a proceedings journal published

by IOP Publishing

Table of contents

Open all abstracts

Preface

Agriculture

Biology

Bioprocessing

Volume 519

2020

Previous issue Next issue

International Conference Earth Science amp Energy 7-8 November 2019 Kuala Lumpur Malaysia

Accepted papers received 01 June 2020

Published online 06 July 2020

011001OPEN ACCESS

Preface

View article PDFOpen abstract

011002OPEN ACCESS

Peer review statement

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Diagnosis Of The Type Of Pests Of Tusam (Pinus Merkusii Jung Et De Vriese)

Fransina Latumahina

View article PDFOpen abstract

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Examination of Pap Smear as A Screening Lesion of Cervical in Muaro Pijoan Village Working Area ofPuskesmas Sungai Duren Muaro Jambi District Jambi Provence

Fairuz Hasna Dewi and Ummi Kalsum

View article PDFOpen abstract

012003OPEN ACCESS

Local Microorganism From Tape (Fermented Cassava) In Composition and Its Effect on Physical ChemicalAnd Biological Quality in Environmental

I Indasah and Abdul Muhith

View article PDFOpen abstract

012004OPEN ACCESS

Effective Consumption of Garlic (Allium Sativum Linn) on Decreasing Blood Cholesterol Levels

Siti Maisaroh Chilyatiz Zahro Dewi Retno Puspitosari Achmad Wahdi and Tiara Fatma Pratiwi

View article PDFOpen abstract

Computer Science

012005OPEN ACCESS

Effectiveness of Consumption Sechium Edule on Decreasing Blood Pressure in Elderly with Hypertension inCoastal Area

Rista Fauziningtyas Arvian Cahya Adi Ristanto and Makhfudli

View article PDFOpen abstract

012006OPEN ACCESS

Fatty Acid Levels of Lemuru Fish Flour and Pumpkin Yellow Tryptophan Levels in Autistic Children Cookies

Estuasih Dyah Pertiwi Moch Sulchan Dwi Pudjonarko Annastasia Ediati Enik Sulistyowati and Ria Ambarwati

View article PDFOpen abstract

012007OPEN ACCESS

The Effectiveness of Giving Papaya Fruit (Carica Papaya) Toward Blood Pressure on Elderly HypertensionPatients

Achmad Wahdi Puji Astuti Dewi Retno Puspitosari Siti Maisaroh and Tiara Fatma Pratiwi

View article PDFOpen abstract

012008OPEN ACCESS

The Influence of Propolis Compress on Phelebitis Score Reduction in Hospitalized Patients

A Fudhali HR Hakim Tobroni Meggy Wulandari Kai Novia Susanti and H M Salim

View article PDFOpen abstract

012009OPEN ACCESS

In Vitro Sunscreen Activity of Red Lettuce Extract (Lactuca sativa var crispa L)

J Rohmah F E Wulandari and C S Rini

View article PDFOpen abstract

012010OPEN ACCESS

The Growth of Moringa Seedling Originated from Various Sizes of Stem Cutting

Bambang Budi Santoso and IGM Arya Parwata

View article PDFOpen abstract

012011OPEN ACCESS

Yield Performances of Jatropha curcas L Originating from Multi-entrenched Seedlings through Two YearProduction Cycles

Bambang Budi Santoso and IGM Arya Parwata

View article PDFOpen abstract

012012OPEN ACCESS

Study of Red Dragon Fruit Peel (Hylocereus Polyrhizus) Extract as Natural Food Colorants to PhysicochemicalProperties Of Pedadas Jam as Functional Foods

Addion Nizori and Lamtiar

View article PDFOpen abstract

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View article PDFOpen abstract

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Environmental Application

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View article PDFOpen abstract

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Akas Yekti Pulih Asih Abdul Muhith Abdul Hakim Zakkiy Fasya and H Hermanto

View article PDFOpen abstract

012018OPEN ACCESS

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View article PDFOpen abstract

012019OPEN ACCESS

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Elfizar Sukamto A Fitriansyah G Sastria and M Erna

View article PDFOpen abstract

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Elfrida Ratnawati

View article PDFOpen abstract

012021OPEN ACCESS

Building Environmental Legal Culture in Mining Activities in Belitung Timur

Dwi Haryadi Darwance and Putra Pratama Saputra

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View article PDFOpen abstract

012022OPEN ACCESS

Environmental Investment Protection in The Era of Industrial Revolution 40

Pranoto Kukuh Tejomurti and Munawar Kholil

View article PDFOpen abstract

012023OPEN ACCESS

Law Enforcement of Environmental Pollution and Damage

Rani Hendriana Nurani Ajeng Tri Utami and Angkasa

View article PDFOpen abstract

012024OPEN ACCESS

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Andrean Eka Lucianto and Herdis Herdiansyah

View article PDFOpen abstract

012025OPEN ACCESS

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Ignatia Martha Hendrati and Achmad Room Fitrianto

View article PDFOpen abstract

012026OPEN ACCESS

Compressive and tensile strength of bamboo species

M A P Handana B Surbakti D D Harisdani R Karolina and T F Rizki

View article PDFOpen abstract

012027OPEN ACCESS

Factors Affecting the Occurrence of Suspected Contact Dermatitis in the Traditional Fishery Processing Area(PHPT) of Muara Angke

Frida Ayu Nawangsih Nunuk Nugrohowati and Erna Harfiani

View article PDFOpen abstract

012028OPEN ACCESS

Desirable Ecotourism Plan for Pulau Ubin in Singapore towards sustainable development to support Singaporetourism industry

Nunuk Nugrohowati

View article PDFOpen abstract

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Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

Table of contents

Open all abstracts

Preface

Agriculture

Biology

Bioprocessing

Volume 519

2020

Previous issue Next issue

International Conference Earth Science amp Energy 7-8 November 2019 Kuala Lumpur Malaysia

Accepted papers received 01 June 2020

Published online 06 July 2020

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Cu (Cuprum) Pollution Effect from Shrimp Processing

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Fenty U Puluhulawa and Amanda Adelina Harun

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Paramitha Amelia Kusumawardani Cholifah and Hanik Machfudlo

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Suprajitno and Sri Mugianti

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M Isnawati M D Larasati D L P Muninggar D N Afifah L B Suromo and M Sulchan

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Fadjar Kurnia Hartati Arlin Besari Djauhari and Sri Satya Antarlina

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S Susilowati D N Martono S Wahyono and H Herdiansyah

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Ika Yuni Wulansari Tiodora Hadumaon Siagian and Ernawati Pasaribu

View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

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View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
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Cu (Cuprum) Pollution Effect from Shrimp Processing

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Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

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Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

Food and Nutrition

Food Science

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Fenty U Puluhulawa and Amanda Adelina Harun

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Palm Date Increase Adolescents Hemoglobin Levels

Paramitha Amelia Kusumawardani Cholifah and Hanik Machfudlo

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The Individuals Knowledge at Risk to Suffered of Non Communicable Disease

Suprajitno and Sri Mugianti

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The Effect of Processed Tempeh Gembus Administration on Blood Glucose in Obese Women

M Isnawati M D Larasati D L P Muninggar D N Afifah L B Suromo and M Sulchan

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Ida Agustini Saidi Agus Miftakhurrohmat Fitria Eka Wulandari Syarifa Ramadhani Nurbaya and Ardin Widianto

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Hanifah Ihsaniyati Nuning Setyowati and Pardono

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Influence of attack intensity of Sucking Pod (RiptorusLinearis) to the Yield of Superior Soybean Varieties inDrought Stress Condition

Muhammad Sarjan

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Heny Herawati Elmi Kamsiati and Muchamad Bachtiar

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Heny Herawati Elmi Kamsiati and Muchamad Bachtiar

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Egi S Avianto Budi Prasojo Ega Sepfriansyah A Ali Imron Endah Wismawati Burniadi Moballa Faiz Hamzah and Ayu Rossa AN

View article PDFOpen abstract

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Vibration Analysis of Ship-RUV Structure in Operational Conditions

Mohammad Basuki Rahmat Imam Sutrisno Budianto Ari Wibawa Budi Santosa and Faris Nofandi

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Ari Wibawa Budi Santosa Septian Wahyu Saputra Imam Sutrisno Ari Yudha Lusiandri and Faris Nofandi

View article PDFOpen abstract

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Design of Coal Handling System for Filling Bungker using PID Method in Coal PLTU

Imam Sutrisno Rafidan Maulana Sudibyo Hendro Agus Widodo Moch Rifai Agus Dwi Santoso and Anak Agung Istri Sri Wahyuni

View article PDFOpen abstract

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Soil Science

Sustainable Development

Waste Management

012048OPEN ACCESS

Estimation of Oil Recovery for Hydrocarbon Injection EOR Method using a Newly-Developed PredictiveModel

M G Merdeka Z Syihab S D Rahmawati A H L Gaol A Yasutra M B Setiawan F Metra D T Winata V Sitompul M C Aziz et al

View article PDFOpen abstract

012049OPEN ACCESS

The Influence Of Plant Age On Growth And Quality Of Plant Nilam Oil (Pogostemon Cablin Benth) UnderThe Improvement Of Palm Oil Plant

Anis Tatik Maryani Fetty Febriasti Bahar and Rikky Herdiyansyah

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Review Regulation on The Determination of Fly Ash and Bottom Ash from Coal Fired Power Plant asHazardous Waste in Effort to Increase Utilization in Indonesia

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View article PDFOpen abstract

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Effects of land use on sedimentation rates at Cimanuk watershed West Java

I Munfarida M Munir and A Rezagama

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Soil deformation analysis by using plaxis 2D that cause by vibration of pilling hammer

F Syarif M Kurniawan and Elizar

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Analytic Hierarchy Process Method for Minimize Negative Sustainability and Environmental Impacts

Ribangun Bamban Jakaria Heri Widodo N Haizal and M B Ibrahim

View article PDFOpen abstract

012055OPEN ACCESS

A Challenge of Inorganic Solid Waste Reduction Practices in Suburban Area

S Susilowati D N Martono S Wahyono and H Herdiansyah

View article PDFOpen abstract

JOURNAL LINKS

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Journal scope

Information for organizers

Information for authors

Contact us

Reprint services from Curran Associates

012056OPEN ACCESS

Analysis Correlation of Municipal Solid Waste Generation and Population Environmental Perspective

Sudiatmoko Supangkat and Herdis Herdiansyah

View article PDFOpen abstract

012057OPEN ACCESS

Estimation of households that manages their domestic waste in not eco-friendly ways in municipal level inIndonesia using EBLUP Fay-Herriot model

Ika Yuni Wulansari Tiodora Hadumaon Siagian and Ernawati Pasaribu

View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

Food Technology

Marine Technology

Oil Science

View article PDFOpen abstract

012040OPEN ACCESS

Food control and biosecurity roles in the global value chain supporting producers or safeguarding consumers

Dikky Indrawan and Arief Daryanto

View article PDFOpen abstract

012041OPEN ACCESS

Implementation of Statistical Process Control Method and Root Cause Analysis on Quality of Bitter Tannin TeaTin

Wiwik Sulistiyowati Danang Tri Handoko and Hana Catur Wahyuni

View article PDFOpen abstract

012042OPEN ACCESS

Mung bean Sprouts skin waste process for environmental sustainability

Fadjar Kurnia Hartati Arlin Besari Djauhari and Sri Satya Antarlina

View article PDFOpen abstract

012043OPEN ACCESS

Food Safety Monitoring System using IoT in the Poultry Slaughterhouse

Nur Hasanah and Dikky Indrawan

View article PDFOpen abstract

012044OPEN ACCESS

Pipeline Corrosion Protection Simulation of Cathodic Protection Method Againts Electrochemical PotentialDistribution

Egi S Avianto Budi Prasojo Ega Sepfriansyah A Ali Imron Endah Wismawati Burniadi Moballa Faiz Hamzah and Ayu Rossa AN

View article PDFOpen abstract

012045OPEN ACCESS

Vibration Analysis of Ship-RUV Structure in Operational Conditions

Mohammad Basuki Rahmat Imam Sutrisno Budianto Ari Wibawa Budi Santosa and Faris Nofandi

View article PDFOpen abstract

012046OPEN ACCESS

Detection of Oil and Filter Feasibility in Ship Gearbox with SVM (Support Vector Machine) Method Based onMicrocontroller

Ari Wibawa Budi Santosa Septian Wahyu Saputra Imam Sutrisno Ari Yudha Lusiandri and Faris Nofandi

View article PDFOpen abstract

012047OPEN ACCESS

Design of Coal Handling System for Filling Bungker using PID Method in Coal PLTU

Imam Sutrisno Rafidan Maulana Sudibyo Hendro Agus Widodo Moch Rifai Agus Dwi Santoso and Anak Agung Istri Sri Wahyuni

View article PDFOpen abstract

Renewable Energy

Soil Science

Sustainable Development

Waste Management

012048OPEN ACCESS

Estimation of Oil Recovery for Hydrocarbon Injection EOR Method using a Newly-Developed PredictiveModel

M G Merdeka Z Syihab S D Rahmawati A H L Gaol A Yasutra M B Setiawan F Metra D T Winata V Sitompul M C Aziz et al

View article PDFOpen abstract

012049OPEN ACCESS

The Influence Of Plant Age On Growth And Quality Of Plant Nilam Oil (Pogostemon Cablin Benth) UnderThe Improvement Of Palm Oil Plant

Anis Tatik Maryani Fetty Febriasti Bahar and Rikky Herdiyansyah

View article PDFOpen abstract

012050OPEN ACCESS

Increase Electricity Supply to the Community to Support Sustainable Development by Minimizing House Loadin Geothermal Power Plant

Raity Arief Hidajat Suyud Warno Utomo Iwa Garniwa and Mahawan Karuniasa

View article PDFOpen abstract

012051OPEN ACCESS

Review Regulation on The Determination of Fly Ash and Bottom Ash from Coal Fired Power Plant asHazardous Waste in Effort to Increase Utilization in Indonesia

Mekkadinah Suyud Suwarno Iwa Garniwa and Haruki Agustina

View article PDFOpen abstract

012052OPEN ACCESS

Effects of land use on sedimentation rates at Cimanuk watershed West Java

I Munfarida M Munir and A Rezagama

View article PDFOpen abstract

012053OPEN ACCESS

Soil deformation analysis by using plaxis 2D that cause by vibration of pilling hammer

F Syarif M Kurniawan and Elizar

View article PDFOpen abstract

012054OPEN ACCESS

Analytic Hierarchy Process Method for Minimize Negative Sustainability and Environmental Impacts

Ribangun Bamban Jakaria Heri Widodo N Haizal and M B Ibrahim

View article PDFOpen abstract

012055OPEN ACCESS

A Challenge of Inorganic Solid Waste Reduction Practices in Suburban Area

S Susilowati D N Martono S Wahyono and H Herdiansyah

View article PDFOpen abstract

JOURNAL LINKS

Journal home

Journal scope

Information for organizers

Information for authors

Contact us

Reprint services from Curran Associates

012056OPEN ACCESS

Analysis Correlation of Municipal Solid Waste Generation and Population Environmental Perspective

Sudiatmoko Supangkat and Herdis Herdiansyah

View article PDFOpen abstract

012057OPEN ACCESS

Estimation of households that manages their domestic waste in not eco-friendly ways in municipal level inIndonesia using EBLUP Fay-Herriot model

Ika Yuni Wulansari Tiodora Hadumaon Siagian and Ernawati Pasaribu

View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

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Estimation of Oil Recovery for Hydrocarbon Injection EOR Method using a Newly-Developed PredictiveModel

M G Merdeka Z Syihab S D Rahmawati A H L Gaol A Yasutra M B Setiawan F Metra D T Winata V Sitompul M C Aziz et al

View article PDFOpen abstract

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The Influence Of Plant Age On Growth And Quality Of Plant Nilam Oil (Pogostemon Cablin Benth) UnderThe Improvement Of Palm Oil Plant

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Effects of land use on sedimentation rates at Cimanuk watershed West Java

I Munfarida M Munir and A Rezagama

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Soil deformation analysis by using plaxis 2D that cause by vibration of pilling hammer

F Syarif M Kurniawan and Elizar

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Analytic Hierarchy Process Method for Minimize Negative Sustainability and Environmental Impacts

Ribangun Bamban Jakaria Heri Widodo N Haizal and M B Ibrahim

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A Challenge of Inorganic Solid Waste Reduction Practices in Suburban Area

S Susilowati D N Martono S Wahyono and H Herdiansyah

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Analysis Correlation of Municipal Solid Waste Generation and Population Environmental Perspective

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Estimation of households that manages their domestic waste in not eco-friendly ways in municipal level inIndonesia using EBLUP Fay-Herriot model

Ika Yuni Wulansari Tiodora Hadumaon Siagian and Ernawati Pasaribu

View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

JOURNAL LINKS

Journal home

Journal scope

Information for organizers

Information for authors

Contact us

Reprint services from Curran Associates

012056OPEN ACCESS

Analysis Correlation of Municipal Solid Waste Generation and Population Environmental Perspective

Sudiatmoko Supangkat and Herdis Herdiansyah

View article PDFOpen abstract

012057OPEN ACCESS

Estimation of households that manages their domestic waste in not eco-friendly ways in municipal level inIndonesia using EBLUP Fay-Herriot model

Ika Yuni Wulansari Tiodora Hadumaon Siagian and Ernawati Pasaribu

View article PDFOpen abstract

Content from this work may be used under the terms of the Creative Commons Attribution 30 licence Any further distributionof this work must maintain attribution to the author(s) and the title of the work journal citation and DOI

Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

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Published under licence by IOP Publishing Ltd

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

1

Cu (Cuprum) Pollution Effect from Shrimp Processing

Akas Yekti Pulih Asih1 Abdul Muhith1 Abdul Hakim Zakkiy Fasya1 H Hermanto2

1Nahdlatul Ulama University of Surabaya Indonesia 2Universitas Panca Marga Indonesia

abdulmuhithunusaacid

Abstract Litopenaeus vannamei shrimp is a type of aquaculture shrimp easily

maintained in fresh water short-lived at a lower cost compared to other types of

shrimp cultivation So vannamei shrimp is more available in traditional markets The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a

cuprum (Cu) mixture The study was conducted by taking samples of Litopenaeus

vannanmei species purchased in 8 traditional markets spread throughout the city of

Surabaya Each market is taken 5 random samples of shrimp with relatively the same

size and condition Then it is processed by steaming boiling frying and burning

Determination of Cu heavy metal content in shrimp is carried out using atomic

absorption type AA 3e00 Variant Tech tron (AAS) with a few additional 50 ml glass

cups 10 ml volumetric flask 5 ml polyethylene vial 10-100 effendorf micropipette microl

and analytical balance There was a significant difference between boiling (p = 0000)

and steamed (p = 0001) with shrimp in raw conditions Whereas for processing by

grilling (p = 0989) and by frying (p = 0078) showed insignificant results This means

that there is no significant difference in Cu content in shrimp between raw conditions

and processing by being grilled and fried

1 Introduction

Shrimp is one of the main commodities of Indonesian aquaculture so it is expected to support the

countrys economy from the fisheries sector In addition to cultivation technology it is known that this

shrimp has a market both at home and abroad and is a major factor in increasing the growth of shrimp

farming in Indonesia Based on production volume in the last 5 (five) years national shrimp

production showed a positive growth trend with an average annual growth of 157 Percent(1)

Litopenaeus vannamei shrimp (better known as vannamei shrimp) is a type of cultivated shrimp It is a

type of shrimp that is easily maintained in fresh water short-lived at a lower cost compared to other

types of shrimp cultivation So that this type of shrimp is more available in traditional markets with

prices relatively cheaper when compared to other types of shrimp both living and dead The

cultivation of vannamei shrimp uses fisheries technology in its feed which is a cuprum (Cu)

mixture(23)

Copper content Cu is the second highest in the head and skin profile of shrimp Cu plays an important

role in oxygen-carrying hemocyanins which are the same as hemoglobin in vertebrate blood

However it can become toxic when it exceeds a certain threshold Cu is the cheapest and most

commonly used pesticide in the aquaculture industry and other aquatic systems(4) Most of the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

2

pesticides in aquaculture use copper as the active ingredient that can contribute to the presence of

copper in the aquatic environment because it settles on the pond bottom(5)

2 Materials And Methods

This research uses a laboratory test design by providing household treatment in Litopenaeus vannamei

there were steamed boiled fried and grilled The results will be analyzed for differences in the levels

of Cu heavy metals Using shrimp originating from 8 traditional markets with each market taken 5

sample shrimp This study used a cross-sectional approach data collection for all variables is done at

one time in order to obtain relatively homogeneous shrimp

3 Results And Discussion

The results of examination of Cu content in Litopenaeus vannamei shrimp showed that in raw

conditions Cu content had an average of 127 ppm with a deviation of 098 ppm Based on the

regulations of the Indonesian Food and Drug Supervisory Agency it states that the maximum limit of

heavy Cu metals in fish food and the results of its processing is 20 ppm(6) So that it can be stated that

the Cu content in vannamei shrimp raw conditions is still within the safe limits allowed

Table 1 Cu Content In Litopenaeus vannamei Based on Household Treatment

Market Source Cu content (ppm)

Raw Steamed Boiled Fried Grilled

B1 1275 1198 859 1106 1162

B2 1276 992 979 1321 1067

K1 1492 1267 1076 1187 1362

K2 1232 1087 1084 1145 1398

K3 1177 1123 998 1207 1376

K4 1202 998 1072 1123 1401

K5 1344 1112 1103 1198 1234

K6 1184 881 1021 1143 1176

Average 1273 1082 1024 1179 1272

Difference 191 249 094 001

Table 1 shows that the average Cu content after processing shows different results The best results

with the lowest Cu content are processed by boiling with an average difference in Cu content of 249

ppm compared to the raw condition Processing by steaming also shows good results because it can

reduce Cu content from raw conditions by an average of 191ppm While processing by frying

successfully reduces the Cu content in vannamei shrimp by an average of 094 ppm However grilled

processing does not show a real reduction because it is only able to reduce the average Cu content by

001 ppm

Table 2 Statistical Difference in Cu Content

(I)

treatment

(J)

treatment

Sig Mean

Difference

(I-J)

Std

Error

95 Confidence

Interval

Lower

Bound

Upper

Bound

Raw Steamed 001 190500 51824 8529 29571

Boiled 000 248750 51824 14354 35396

Fried 078 94000 51824 -1121 19921

Grilled 989 00750 51824 -10446 10596

To ascertain the results of Cu content in Litopenaeus vannamei shrimp a statistical test was carried

out So that it can be seen that the significant difference in Cu content between the processing results

compared with the raw conditions Based on table 2 it is known that significant differences were

obtained from the results of processing by boiling (p = 0000) and steaming (p = 0001) Whereas the

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)

International Conference Earth Science amp Energy

IOP Conf Series Earth and Environmental Science 519 (2020) 012017

IOP Publishing

doi1010881755-13155191012017

3

processing that shows the results of Cu is not significant namely by frying (p = 0078) and grilled (p =

0989)

4 Conclusion

The results of the study of differences in the residual Cu levels in shrimp in the Surabaya traditional

market based on household treatment there was significant differences in the Cu content in

Litopenaeus vannamei shrimp The best processing of Litopenaeus vannamei shrimp is by boiling or

steaming because it can reduce copper significantly The worst processing of Litopenaeus vannamei

shrimp is by grilled because it does not cause a significant difference in Cu content compared to raw

shrimp

Significance Statements

This study discovers the potential effect of Cu pollution risks among consumer of vannamae shrimp

This study help the researchers and communities to understand and manage the Cu pollution in

initially reducing the Cu hazard before generate seriously health and environment problem Thus a

new feed technology and regulation of shrimp feed need to be applied as the instrument for decision

makers to set good Cu elimination program in shrimp

Acknowledgement

All researchers are grateful to Nahdlatul Ulama University of Surabaya who have given of funding

support during the research and publication and thank to Balai Besar Laboratorium Kesehatan

Surabaya for conducting the copper heavy metal analysis in accordingly

Conflict of Interest no conflict of interest need to be reported in this study

Ethical Clearance Obtained from Faculty of Health Committee

Source of Funding Nahdlatul Ulama University of Surabaya

References

[1] Kementerian Kelautan dan Perikanan (KKP) BUDIDAYA UDANG MASIH SANGAT

POTENSIAL [Internet] 2018 [cited 2019 Jul 29] Available from

httpskkpgoiddjpbartikel8688-kkp-budidaya-udang-masih-sangat-potensial

[2] Asih AYP Irawan B Soegianto A Effect of copper on survival osmoregulation and gill

structures of freshwater prawn ( Macrobrachium rosenbergii de Man) at different development

stages Mar Freshw Behav Physiol 2013 Mar46(2)75ndash88

[3] Martiacutenez A Romero Y Castillo T Mascaroacute M Loacutepez-Rull I Simotildees N et al The Effect of

Copper on the Color of Shrimps Redder Is Not Always Healthier McGraw K editor PLoS

ONE 2014 Sep 179(9)e107673

[4] Chen W-Y Ju Y-R Lin C-J Tsai J-W Chen S-C Liao C-M Environmental stochasticity

promotes copper bioaccumulation and bioenergetic response in tilapia Stoch Environ Res Risk

Assess 2015 Aug29(6)1545ndash55

[5] European Commission Directorate-General for the Environment University of the West of

England (UWE) Science Communication Unit Sustainable aquaculture [Internet]

Luxembourg Publications Office 2015 [cited 2019 Jul 29] Available from

httpbookshopeuropaeuuritarget=EUBNOTICEKHBB14004ENHTML

[6] BPOM Batas maksimum Cemaran Logam dalam Makanan DIREKTUR JENDERAL

PENGAWASAN OBAT DAN MAKANAN 1989

  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011001pdf (p1-2)
  • _2020_IOP_Conf_Ser__Earth_Environ_Sci_519_011002pdf (p3-4)
  • Asih_2020_IOP_Conf_Ser__Earth_Environ_Sci_519_012017pdf (p5-8)