ICAR - CTRI

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i ICAR - CTRI Annual Report 2015-16

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i ICAR - CTRI Annual Report 2015-16

ICAR - CTRI Annual Report 2015-16 ii

iii ICAR - CTRI Annual Report 2015-16

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ICAR-CENTRAL TOBACCO RESEARCH INSTITUTERAJAHMUNDRY - 533 105, ANDHRA PRADESH, INDIA

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2015-16ANNUAL REPORT

ICAR - CTRI

ICAR - CTRI Annual Report 2015-16 iv

Published by

Dr. D. Damodar ReddyDirectorICAR-Central Tobacco Research InstituteRajahmundry - 533 105, Andhra Pradesh, IndiaPhone: 0883-2449871-4, Fax: 0883-2448341, 2410555e-mail : [email protected] : www.ctri.org.in

Edited byDr. D. Damodar ReddyDr. C. ChandrasekhararaoDr. A.V.S.R. SwamyDr. P. VenkateswarluDr. K. Suman Kalyani

Compiled byDr. K. SaralaC.V.K. Reddy

Assisted byCh. LakshminarayaniMd. Elias

Printed atNew Image Graphics, Vijayawada-520 002Phones : 0866 2435553

All rights reserved. No part of thispublication may be reproduced ortransmitted in any form by print, microfilmor any other means without the writtenpermission of the Director, ICAR-CTRI.

Annual ReportƒÁu |N˛ üuoƒztå

2015-16

ICAR-CTRI

v ICAR - CTRI Annual Report 2015-16

Preface .........................................................................................i

dk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”k .............................................................................01

Executive Summary............................................................................06

Introduction ...................................................................................11

Research Achievements .....................................................................16

Technology Assessed and Transferred .....................................................57

Education and Training .....................................................................59

Krishi Vigyan Kendra .........................................................................67

Awards and Recognitions ....................................................................69

Linkages and Collaborations ...............................................................70

All India Network Project on Tobacco ......................................................72

Empowerment of Women in Agriculture ...................................................75

List of Publications ..........................................................................78

List of Approved On-going Projects ........................................................80

RAC, QRT, IRC and IMC Meetings ...........................................................83

Participation of Scientists in Conferences, ...............................................86Meetings, Workshops and Symposia

Trainings and Capacity Building ............................................................90

Workshops, Seminars and Farmers’ Days ..................................................92Organised by the Institute

Distinguished Visitors .......................................................................94

Personnel .....................................................................................96

Appendix 1: RFD for ICAR-CTRI (2014-2015) ...........................................100

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The year 2015-16 has been most invigorating in theannals of ICAR-Central Tobacco Research Instituteas it witnessed the institute being granted the

prestigious ISO 9001:2008 certificate for successfulimplementation of the quality management system withregard to basic, strategic and applied research on tobaccocrop. The Vision-2050 document of the Institute was alsoprepared during the year. This document articulates the

Institute’s long-term vision in terms of providing vibrant research back-up for Indian tobacco to beless harmful, remunerative and globally competitive in the changing milieu of national andinternational policy regimes.

It is indeed an honour for me to present before you the Annual Report of the ICAR – CTRI for the year2015-16. The institute has always been in the forefront to provide research services to the tobacco farmingcommunity by evolving varieties of different tobacco types for high yield potential coupled with qualityand other desirable traits, and by developing suitable agro-techniques for effective management ofresources, pesticide residues and a multitude of biotic and abiotic stresses.

During the year 2015-16, the institute facilitated the quinquennial review of its research programmesfor the period from 2009-10 to 2013-14 by the ICAR appointed QRT team headed by Dr. R. R. Hanchinal,Chairperson, PPV&FRA, New Delhi. The institute’s Research Advisory Committee with Dr. A. Padma Raju,Vice-Chancellor, ANGRAU as its Chairman also reviewed the institute’s research and made itsrecommendations for strengthening and fine tuning the ongoing research and initiating new programmesto address the emerging issues relevant to tobacco sector.

The most significant research accomplishments made during the year 2015-16 include: Release ofthree FCV tobacco varieties (Lt Kanchan, CH1 and N 98) and one bidi tobacco variety (Nandyal Pogaku-1)for tobacco growing areas of Andhra Pradesh; Enriching and maintenance of tobacco germplasm;Development of DUS descriptors for characterising tobacco varieties; Use of bio-mass ashes as sources fornutrient supplementation; Management intervention for orobanche control; Evaluation and identificationof effective pesticide molecules; Development of IPM strategies and pesticide application techniques andidentification of neutral volatile aroma compounds in tobacco. The two Krishi Vigyan Kendras at Kalavacharlaand Kandukur have made significant contribution in terms of technology assessment, front-linedemonstrations, women empowerment, income generation activities and trainings for skill sharpeningand capacity development for the benefit of farming community of their respective operational domains.The other significant services provided by the institute to the farming community during the year include:supply of about 6000 kgs of tobacco seed to farmers, and issuing of more than 600 nos. of Soil HealthCards to the farmers of A.P. and Karnataka on the eve of World Soil Day. A National Seminar on CropDiversification, sponsored by DAC&FW, MoA & FW, GoI was conducted on 18-1-2016 and discussed themyriad issues relating to crop diversification. Under Tribal Sub-plan:2015-16, a Tribal Agri Action Plancovering important inputs and management intervention was prepared and implemented atManchulavarigudem and Sugalimetta in A.P. Lastly, it is heartening to note that some Scientists havebrought laurels to the institute by way of bagging prestigious honours and awards in recognition of theiroutstanding research contributions.

I express my gratitude to Dr. Trilochan Mohapatra, Secretary, DARE & DG, ICAR, and Dr. S. Ayyappan,the Former Secretary, DARE & DG, ICAR for their inspirational leadership and guidance. I place on recordmy sincere thanks to Dr. J.S. Sandhu, DDG (Crop Science), Dr. R.K.Singh, ADG (CC) & Dr. P.K.Chakraborty,Former i/c ADG (CC) for their motivation and support extended from time to time. The encouragementand guidance given by the Dr. R.R. Hanchinal, Chairman, QRT and Dr. A. Padma Raju, Chairman, RAC aregratefully acknowledged. I appreciate the sincere efforts made by the editorial committee, PME cell andLDS in bringing out the annual report in time. Finally, I thank all the Heads of the Divisions/ResearchStations, scientists and technical & administrative staff for their help and cooperation in running theinstitute smoothly and effectively.

(D. DAMODAR REDDY) Director

15th June, 2016Rajahmundry

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dk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”kdk;Zdkjh lkjka”k

rEckdw fdLeksa dk fodklrEckdw fdLeksa dk fodklrEckdw fdLeksa dk fodklrEckdw fdLeksa dk fodklrEckdw fdLeksa dk fodkl

fjyht fd, x, fdLesafjyht fd, x, fdLesafjyht fd, x, fdLesafjyht fd, x, fdLesafjyht fd, x, fdLesa

● O;olkf;d [ksrh ds fy, pkj rEckdw fdLeksa dksfjyht fd;k x;k uker% ¶ysoj;qä ,Qlhoh rEckdwladj lh,p&1] ,Qlhoh rEckkdw fdLesa ,yVhdapu ¼de Vkj okyk fdLe ½ ,oa ,u&98 rFkkchM+h rEckdw fdLe uanh;kyk iksxkdw&1A pkjksa fdLeksaesa mPp mit {kerk vkSj csgrj xq.koÙkk gSA

fdLeksa esa lq/kkjfdLeksa esa lq/kkjfdLeksa esa lq/kkjfdLeksa esa lq/kkjfdLeksa esa lq/kkj

● dkyh e‘nk & mUur çtuu oa’kØe oh&5057]vkj,l&27 rFkk vkj,l&29 esa lkekU; fdLefljh dh rqyuk esa mipkfjr iÙkh mit] lqo.kZ iÙkhmit rFkk Js.kh lwpdkad mYys[kuh; :i ls vf/kd ik;h x;hA fljh dh rqyuk esa bu oa’kØeksa dhmit esa vkSlru mipkfjr iÙkh esa 19%] lqo.kZiÙkh esa 23% vkSj Js.kh lwpdkad esa 20% dho‘f) gqbZA

● mRrjh gYdh e‘nk & lksekDyksUl] ,u,ylhvkj&1&9&2&13 ,oa ,u,ylhvkj&7&11&1&4] mUurçtuu oa’kØe vkjVh3&1 ,oa vkjVh4&3 esa dapudh rqyuk esa vf/kd mit ik;h x;h ftllsmipkfjr iÙkh mit esa 20&41% vkSj Js.kh lwpdkadesa 41&65% o‘f) gqbZA

● dukZVd gYdh e‘nk & mUur çtuu oa’kØe,Qlh,p 239 rFkk ,Qlh,p 242 esa vf/kdremipkfjr iÙkh ¼2600 rFkk 2579 fd-xzk-½] lqo.kZiÙkh ¼1443 rFkk 1716 fd-xzk-½ vkSj VhthbZ ¼Øe’k%1409 rFkk 1708½ ntZ dh xbZA

● flafpr ukVw rEckdw & mUur çtuu oa’kØe ,u,Q3&5&1 rFkk ,u,Q 3&6&2 esa lkekU; fdLedksEewkxwMse dh mit dh rqyuk esa mipkfjr iÙkhmit ¼19&59%½ mYys[kuh; :i ls vf/kd ntZdh xbZA

● poZ.k rEckdw & lkekU; fdLeksa esa csgrj fdLevfcjkeh dh rqyuk esa p;u ,poh 2011&2 esamYys[kuh; :i ls iÙkh mit vf/kd ¼11-0% dho‘f)½ ik;h x;hA

● ladj rEckdw & lkekU; fdLe fljh dh rqyukesa 10 lh,e,l ladjksa esa egRoiw.kZ ekud fHkUukJ;¼gsVsjksfll½ ns[kk x;kA mipkfjr iÙkhs ds lanHkZ esafHkUukJ; 18&34] lqo.kZ iÙkhs ds lanHkZ esa 14&35rFkk Js.kh lwpdkad ds lanHkZ 17&36 ntZ fd;kx;kA

● jksx çfrjksf/krk & fjyht fd, x, fdLeksa uker%fljh] ,u&98 rFkk lhokbZ&142 esa CySd ‘ksad çfrjksf/krk xq.k lfEefyr fd;k x;kA nks lkS rSarhl larfr;ksaesa CySd ‘ksad çfrjksf/krk ntZ dh xbZA

tuuæO; lalk/ku çca/kutuuæO; lalk/ku çca/kutuuæO; lalk/ku çca/kutuuæO; lalk/ku çca/kutuuæO; lalk/ku çca/ku

● Hkk—vuqi&dsUæh; rEckdw vuqla/kku laLFkku esamiyC/k vkuqoaf’kd lalk/kuksa dh dqy la[;k 3369oa’kØe gSA

● oU;; fudksfV;kuk çtkfr;ka & 56 oU; fudksfV;kukçtkfr;ksa rFkk 2 miçtkfr;ksa ds dqy 210 oa’kØe]9 fons’kh ladj] 5 yksdy bUVj LisflfQd gkbfczM~lrFkk 4 ,Eiyh fMIykl;M~l dk j[kj[kko fd;kx;kA —f=e ifjos’k esa eSØksçksikxs’ku ds ek/;e ls11 Qwy u f[kyus okys oa’kØeksa dks cpk;k x;kA

● Mh;w,l fp=hdj.k & rhu thuç:iksa uker% fljh]Vhch,lVh&2 rFkk fof’k”V tuuæO; 324lh dhfHkUurk] lekurk vkSj fLFkjrk ¼Mh;w,l½ ds 53xq.kksa ds fy, xq.kfp=.k fd;k x;kA

rEckdw lq/kkj gsrq tSoçkS|ksfxdhrEckdw lq/kkj gsrq tSoçkS|ksfxdhrEckdw lq/kkj gsrq tSoçkS|ksfxdhrEckdw lq/kkj gsrq tSoçkS|ksfxdhrEckdw lq/kkj gsrq tSoçkS|ksfxdh

● Vh,l,u,] fudksVhu rFkk lksykusl‚y xq.kksa dsfy, 11 ,l,lvkj ekdZj uker% Vh,e51534]V h,e10606 ] Vh,e10891 ] Vh,e50469 ]Vh,e1111062] Vh,e50868] ihVh61653] ihVh10375]ihVh50065] ihVh60723 rFkk ihVh53345 cgq:ihik, x,A

● pkj fMQsal jslikUl thal uker% ,uVh,ebZds2]ihvkj1,] ih,,y1 ds VªkalfØIV fo”ys’k.k lslgfofu;eu Li’V gqvk gS vkSj budk laca/k rEckdwesa lhyu jksx lfg’.kqrk ls gSA

● ,EIyhdkWu dh yEckbZ vkSj vuqØe.k ds lanHkZ esa

ICAR - CTRI Annual Report 2015-16 2

fudksfV;kuk oa”kØeksa es rhoz xfr ls mn~fodflrykslh trnH–psbA ¼bUVjtsfud Lislj jhtu½ cgq:ihik;k x;kA

—f”k&çkS|ksfxfd;ksa dk fodkl—f”k&çkS|ksfxfd;ksa dk fodkl—f”k&çkS|ksfxfd;ksa dk fodkl—f”k&çkS|ksfxfd;ksa dk fodkl—f”k&çkS|ksfxfd;ksa dk fodkl

,Qlhoh rEckdw,Qlhoh rEckdw,Qlhoh rEckdw,Qlhoh rEckdw,Qlhoh rEckdw

● mÙkjh gYdhs e‘nkvksa esa lkekU; lhMfyaXl esa jksi.kds i’pkr e‘nk esa 10] 25&30 rFkk 40&45 fnuksaij moZjd$QjksZ bjhxs’ku dh rqyuk esa Vªs lhMfyaXlij jksi.k ds i’pkr 3] 20&25 rFkk 40&45 fnuksaij Vid flapkbZ djus ij mipkfjr iÙkh mit¼16-67%½ vkSj Js.kh lwpdkad ¼23-44%½ esa o‘f)ns[kh x;hA

● mÙkjh gYdh e‘nkvksa esa csly [kqjkd ds :i esa;wfj;k@veksfu;e lYQsV ¼,,l½ rRi’pkr ;wfj;k;k ,,l ;k ;wfj;k$+,,l ;k ;wfj;k$+,,l+$iksVSf’k;eukbVªsV ;k ;wfj;k+$,,l$+dSfY’k;e ukbVªsV V‚i Mªsflaxds :i esa nsus ij mipkfjr iÙkh mit vkSj Js.khlwpdkad ,d leku gh jgk gSA

● xkscj dh [kkn$,uihds ;k xkscj dh [kkn$vR;af/kd ukbVªkstu$ihds nsus ij vfo’oklh ifjiDorkds fcuk mRikndrk esa o‘f) ns[kh x;hA

● ulZjh esa 25 ;k 50 fd-xzk-@gs- dh rqyuk esa iksVSf’k;evkDlkbM 100 fd-xzk-@gs- dh nj ls pkj Hkkxksa esa¼jksi.k ds nkSjku rFkk jksi.k ds 25] 35 vkSj 45fnuksa ckn½ nsus ij lw[ks inkFkZ dk mPp mRiknurFkk çfr ;qfuV {ks= ls çfrjksi.k ;ksX; uoksnfHknikS/kksa dh vf/kd la[;k ik;h x;hA

● dukZVd dh gYdh e‘nkvksa esa o”kkZ ds fnu vf/kdgksus ij ,Qlhoh rEckdw mRikndrk esa o‘f) rFkkQly vof/k ds nkSjku /kwi ds ?kaVksa ls lglaca/k gSA

● rEckdw dh tSfod [ksrh esa lqo.kZ Js.kh mRiknu esa6&7% dh o‘f)] tSfod rEckdw esa eqj>ku jksxçdksi rFkk tM+ xkaB jksx esa deh gqbZ gSA

● dkyh e‘nk esa mxk, x, rEckdw esa jksi.k ls iwoZisaMhesFkkfyu @ 325 g a.i rFkk vykDykj @ 1000 g a

i/ ha nsus ij mit gkFkksa ls vksjkscaps fudkyus dsleku gh jgk gSA

● oVhZlksy esa xkscj dh [kkn+$,uihds nsus rFkk vksjkscapsfudkyus ij vfo’oklh ifjiDork ds fcuk mPpmipkfjr iÙkh mit vkSj Js.kh lwpdkad çkIr gqbZAVhch,lVh&2 dh rqyuk esa ohVh&1158 fdLe dkfu”iknu vfo’oklh ifjiDork ds fcuk csgrj ik;kx;kA

xSj&,Qlhoh rEckdwxSj&,Qlhoh rEckdwxSj&,Qlhoh rEckdwxSj&,Qlhoh rEckdwxSj&,Qlhoh rEckdw

● lkekU; fdLe esa xkscj dh [kkn 10 Vu@gs- dhrqyuk es a eksrhgkjh rEckdw es a 112 fd-xzk-ukbVªkstu$112 fd-xzk- QkLQkssjl isaVkDlkbM nsusij mPp mipkfjr iÙkh mit ¼2331 fd-xzk-@gs½ntZ dh xbZA

● cyhZ rEckdw esa 100% vkjMh,Q ds lkFk lsV jksIykafVax ¼,lvkjih½ djus ij mYys[kuh; :i lsmPp mipkfjr iÙkh mit rFkk ,u] ih] ds dkmn~xzg.k ns[kk x;kA ,lvkjih esa 50% ;k 75%vkjMh,Q nsus ij 100% vkjMh,Q ds lkFkijEijjkxr jksi.k tSlk gh jgk gSA

● çpfyr vfcjkeh dh vis{kk mUur poZ.k rEckdwoa’kØe ,poh 2009&3 esa çFke Js.kh iÙkh mit esa10-6% rFkk dqy mipkfjr dqy iÙkh mit esa13% dh mYys[kuh; o‘f) gqbZA

● rfeyukMq esa poZ.k rEckdw ikyu esa of”kZd eksfjaxkdks mi;qä varj&Qly ds :i esa igpku dhxbZA

mRiknu {kerk vkSj mRikn xq.koÙkk ds fy, lalk/kumRiknu {kerk vkSj mRikn xq.koÙkk ds fy, lalk/kumRiknu {kerk vkSj mRikn xq.koÙkk ds fy, lalk/kumRiknu {kerk vkSj mRikn xq.koÙkk ds fy, lalk/kumRiknu {kerk vkSj mRikn xq.koÙkk ds fy, lalk/kuvojks/kksa dk çca/kuvojks/kksa dk çca/kuvojks/kksa dk çca/kuvojks/kksa dk çca/kuvojks/kksa dk çca/ku

● Qly vo’ks”kksa dks tykus ls mRiUu ck;ksekl jk[k;k rEckdw mipkj [kfygkuksa ds ydM+h ds jk[k dkmi;ksx ykbfeax eSfVfj;y ds :i esa fd;k tkldrk gS vkSj blls e‘nk dh iksVSf’k;e moZjrk esalq/kkj gksrk gS tks ck;ksekl Mkyh xbZ e‘nk esamiyC/k iksVSf’k;e Lrj ls çekf.kr gksrk gSA

● Qly vo’ks”kksa@ydM+h dh jk[k dks vdsys ;k,lvksih ds lkFk ¼50%$50%½ 100 fd-xzk- iksVSf’k;eds leku vk/kkj ij mi;ksx ls mipkfjr iÙkhmit rFkk iÙkh dh xq.koÙkk dks çHkkfor fd, fcukiksVSf’k;e mn~xzg.k esa mYys[kuh; o‘f) ns[kh xbZA

dk;Zdkjh lkjka”k

3 ICAR - CTRI Annual Report 2015-16

dk;Zdkjh lkjka”k

fofHkUu ck;ksekl jk[k mipkjksa esa rEck~dw dh lkis{kmit dk Lrj galwj esa 88 ls 98% rFkk thyxwfeYyhesa 98 ls 105% ik;k x;kA

● rEckdw MaByksa ls ck;kspkj dk mPp ek=k esa Vhvkslh¼80%½] ukbVªkstu ¼1-23½] QkLQksjl ¼0-78%½]iksVSf’k;e ¼3-48½ rFkk vYdkyhu ih,p ¼9-42½ lsxq.kfp=.k fd;k x;kA rEckdw MaBy ck;kspkj vkSjflaFksfVd ft;ksykbV dh /kuk;u fofue; {kerk¼ds’ku ,Dlpsat dSikflVh½ Øe’k% 30 vkSj 270Cmol (p+) çfr fd-xzk- ik;k x;kA

● ,u- Vckde ls la’ysf”kr flYoj uSuksikVhZdYl dkvkdkj xksykdkj ls vfu;fer rFkk ikVhZdy vkeki10853-3 ,u,e vkSj ihMhvkbZ eku 1-884 ik;kx;kA

● çkFkfed iks”kd rRoksa ¼,u] ih vkSj ds½ dh i;kZIrvkiwfrZ dh fLFkfr esa gh xkS.k iks”kd rRo ikS/ks dho‘f)] p;kip; vkSj iks”kd rRoksa ds mn~xzg.k esaegRoiw.kZ Hkwfedk fuHkkrs gSaA

● chM+h rEckdw ds ukS fdLeksa esa ls ,evkjthVh,p1 esavf/kdre ok”i’khy lqxa/k ;kSfxd ik;k x;kAysuksfyusV chM+h rEckdw esa ekStwn çeq[k vlar‘Irolh; vEy ¼vulSpwjsVsM QSVh ,flM½ gSA

● iÙkh ds fy, rEckdw Qly dSuksih dk LisDVªyfj¶ysDVsal esa laosnu’khy cSaM dh igpku iksVSf’k;eeku 747&862 ,u,e] fudksfVu 539&541 ,u,erFkk 710 ,u,e] ?kVrh ‘kdZjk 631&692 rFkk1901&1970 ,u,e vkSj iÙkh esa dqy ukbVªkstu350&365 ,u,e fd;k x;kA

tSfod LVªSl dk lesfdr çca/kutSfod LVªSl dk lesfdr çca/kutSfod LVªSl dk lesfdr çca/kutSfod LVªSl dk lesfdr çca/kutSfod LVªSl dk lesfdr çca/ku

● e/; ,oa nf{k.kh dkyh e‘nkvksa esa rEckdw ulZjhvkSj jksfir Qly esa iÙkh eqMu vkSj rEckadwlwaMh;ka çeq[k dhV ihM+d gSaA

● dukZVd gYdh e‘nkvksa esa rEckdw ls tqM+s ikapçeq[k ijthoh; lw=—fe;ka ik;h x;ha uker%esyks;Mkstkbu miçtkfr] jksVhysupqyljsuhQkfeZl]gsydksVhysapl miçtkfr] çkVhysapl miçtkfr rFkkVkbysapl miçtkfrA

● ulZfj;ksa ,oa jksfir Qlyksa esa LikssMksiVsjk fyVwjk ds

çfr Dykj¶yktwj‚u 5-4 bZlh 0-03% çHkkoh ik;kx;kA ,Qlhoh rEckdw esa rEckdw ,fQM ekbtlfudksfV;kus ds çfr ¶yksfudkfeM 50 MCY;wth vkSjikbesVªkstkbu 50 MCY;wth 0-02% dh nj lsçHkkoh ik;k x;kA ,Qlhoh rEckdw esa rEckdw OgkbV¶ykbZ csfefl;k Vkckdh ¼xsUuk fM;l½ ijikbesVªkstkbu 50 MCY;wth 0-02% dh nj ls]¶yksfudkfeM 50 MCY;wth 0-02% dh nj ls rFkkLiSjksesflQsu 240 ,llh 0-02% dh nj ls mi;ksxdjus ij çHkkoh ik;k x;kA ¶yksfudkfeM 50MCY;wth 0-02% rFkk ikbesVªkstkbu 50 MCY;wth0-02% fNM+dko okys ,Qlhoh rEckdw esa fNM+dkods 15 fnuksa ckn 1 fe-xzk-@fd-xzk- ls Hkh devo’ks”k ik, x,A

● çfrjksi.k xM~Ms esa uoksnfHkn ikS/ks ds tM+ dksbfeMkDyksfçM 70 ,,Q 0-14% dh nj ij Mqcksus ;kbfeMkDyksjfçM 200 ,l,y 0-005% dh nj lsnsus ij xzkmaM chVy eslkse‚jQl foYyhxj CykudkMZdk çca/ku gks ldrk gSA

● lksdksLiksjk fudksfV;kus ls gksus okys jksx Ý‚x vkbZLi‚V jksx ds çfr iSjkDykslVªksfcu + esVhjke 0-2%;k dkcsaZMkfte 0-05% dk fNM+dko jksx çdksi dsrqjUr ckn vkSj 10&15 fnuksa ds ckn nwljk fNM+dkoçHkkoh ik;k x;kA

● rEckdw ulZfj;ksa esa iÙkh eqj>ku jksx çca/ku ds fy,esVkyDlkby 8% + eSadkstsc 64% dh vis{kkQukfeM‚u 10% + eSadkstsc 50% csgrj fodYigSA

● jksi.k ds i’pkkr 50 fnuksa rd gkbZ Vsd Lçsks;jcsgrj ik;k x;k vkSj blds ckn ,pihds,lfNM+dko æO;i vkSj le; ds lanHkZ esa csgrj gSA

● rEckdw ,fQM ds fu;a=.k esa ck;ks&bUlsasfVo vkbZih,ee‚Mîwy çHkkoh ik;k x;k ftlesa lhek Qly ds:i esa eDdk dh nks iafä;ka] jksi.k ds i’pkroVhZflfy;eyslkuh dk 3X1012 lh,Q;w@gs- dhnj ls ,d fNM+dko rFkk jksi.k ds 60 fnuksa dsi’pkr bZfeMkDyksfçM dk 50 xzk- ,-vkbZ@gs- dkfNM+dko lfEefyr gSA

● mn~Hkkflr ulZjh D;kfj;ksa esa lw=—fe;ksa ds çdksi]

ICAR - CTRI Annual Report 2015-16 4

voeUnu rFkk eqj>ku jksx çdksi ds çHkkodkjhfu;a=.k ds fy, lesfdr e‚M;wy dk fodkl fd;kx;k ftlesa VªkbdksMekZ fojkMs ,oa isflyksekblslfyyklhul] VªkbdksMekZ fojkMs ,oa ikspkfu;kdsfyeMksliksfj;k ds la;kstu dk fjMksfey vkSj¶;wjkM‚u ds lkFk mi;ksx fd;k x;kA

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rEckdw ds oSdfYid mi;ksx dk nksgurEckdw ds oSdfYid mi;ksx dk nksgurEckdw ds oSdfYid mi;ksx dk nksgurEckdw ds oSdfYid mi;ksx dk nksgurEckdw ds oSdfYid mi;ksx dk nksgu

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● nks fnuksa rd 50 fdlkuksa dks feYdh eq’k:e mRiknuesa çf’kf{kr fd;k x;kA

vU; miyfC/k;kavU; miyfC/k;kavU; miyfC/k;kavU; miyfC/k;kavU; miyfC/k;ka

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tutkrh; mi&;kstuktutkrh; mi&;kstuktutkrh; mi&;kstuktutkrh; mi&;kstuktutkrh; mi&;kstuk

● o”kZ 2015&16 ds nkSjku vkU/kz çns’k ds if’pexksnkojh ftys ds lhrIikxwMse xzke iapk;r ds

dk;Zdkjh lkjka”k

5 ICAR - CTRI Annual Report 2015-16

dk;Zdkjh lkjka”k

tutkrh; xkao eapqykokjhxwMse esa vkbZlh,vkj&lhVhvkjvkbZ ds tutkrh; mi&;kstuk dkdk;kZUou;u fd;k x;kA ikap gLr{ksi uker% mPpmit okys eDdk ladj ¼Mh,p&117½ dk QkmuMs’kulhM dh vkiwfrZ] moZjd] ç{ks= midj.k ¼rkSyumidj.k] mUuar fNM+dko ;a=½] LFkk;h [kk|kUuHkaMkj.k xksnkeksa dk fuekZ.k vkfn dk;Z 38 ç{ks=ifjokjksa ds fy, fd;k x;kA

● ,vkbZ,uih dk tutkrh; mi&;kstuk dkdk;kZUo;u vkpk;Z ,u- th- jaxk —f”k fo’ofo|ky;ds vkj,vkj,l] uanh;ky us fd;kA mRikndrk esalq/kkj gsrq laosnu’khy fuos’kksa ¼moZjd] dhVuk’kdvkSj mUur fdLesa½ dh vkiwfrZ] eksVs vukt vkSjnyguksa esa mRikndrk dh o‘f) gsrq çf’k{k.k dk;ZØerFkk lacaf/kr lkfgR; dh vkiwfrZ lfgr vusd çdkjds gLr{ksiksa dk dk;kZUo;u vkU/kz çns’k ds vuariqjftys ds ia;e eaMy ds lqxyhesêk xkao ds 40tutkrh; fdlkuksa ds fy, fd;k x;kA

● vkU/kz çns’k çkS|ksfxdh fodkl vkSj çksUufr dsUæ¼,ih&VhMhlh½] gSnjkckn us vkbZlh,vkj&lhVhvkjvkbZ ds çkS|ksfxdh lg;ksx ls jkteaMªh esafnukad 05-06-2015 ls 07-06-2015 ds nkSjku —f”kçn’kZuh ¼,ih&Vsd 2015½ dk vk;kstu fd;kA

esjk xkao esjk xkSjoesjk xkao esjk xkSjoesjk xkao esjk xkSjoesjk xkao esjk xkSjoesjk xkao esjk xkSjo

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:i ls fdlkuksa ds fy, le; le; ij lykgdkjlsok,a nh tk jgha gSaA

fo’o e‘nk fnolfo’o e‘nk fnolfo’o e‘nk fnolfo’o e‘nk fnolfo’o e‘nk fnol

● lhVhvkjvkbZ ds çknsf’kd dsUæ] galwj] dukZVd]lhVhvkjvkbZ&dsohds] dyokpykZ ¼iwoZ xksnkojhftyk½] vkU/kz çns’k rFkk lhVhvkjvkbZ&dsohds]danwdwj ¼çdk’ke ftyk½] vkU/kz çns’k esa fnukad 05-12-2015 dks fo’o e‘nk fnol euk;k x;kA blvolj ij vkU/kz çns’k vkSj dukZVd ds fdlkuksadks 606 e‘nk LokLF; dkMZ tkjh fd;k x;kA

t; fdlku t; foKku lIrkgt; fdlku t; foKku lIrkgt; fdlku t; foKku lIrkgt; fdlku t; foKku lIrkgt; fdlku t; foKku lIrkg

vkbZlh,vkj&lhVhvkjvkbZ] jkteaMªh esa fnukad23&29 fnlEclj] 2015 ds nkSjku fdlkuksa ds fgr esafoKku dks c<+kok nsus gsrq t; fdlku t; foKkulIrkg dk vk;kstu fd;k x;kA —f”k vkSj lac) {ks=ksaesa ;ksxnku ds fy, uoksUes”kh fdlkuksa vkSj —”kd efgykvksadks lEekfur fd;k x;kA

LoPN Hkkjr dk;ZØeLoPN Hkkjr dk;ZØeLoPN Hkkjr dk;ZØeLoPN Hkkjr dk;ZØeLoPN Hkkjr dk;ZØe

çk—frd lalk/ku çca/ku ¼ty] e‘nk vkSj ÅtkZ½ds laj{k.k ij /;ku dsfUær djrs gq, LoPN Hkkjrdk;ZØe dks dk;kZfUor fd;k x;kA

iqjLdkj ,oa lEekuiqjLdkj ,oa lEekuiqjLdkj ,oa lEekuiqjLdkj ,oa lEekuiqjLdkj ,oa lEeku

● M‚- Mh- nkeksnj jsìh] funs’kd] vkbZlh,vkj&lhVhvkjvkbZ] M‚- ds- lqeu dY;k.kh] ç/kku oSKkfud]M‚- ,p- jfo’kadj dks foKku vkSj çkS|ksfxdh esamRd‘’V ;ksxnku ds fy, çfrf”Vr iqjLdkj vkSjlEekku fn;k x;kA

ICAR - CTRI Annual Report 2015-16 6

Executive Summary

Tobacco Cultivar Development

Varieties released:

● Four tobacco varieties viz.flavourful FCVtobacco hybrid CH-1, FCV tobacco varietiesLT Kanchan (low Tar) and N-98, and biditobacco variety Nandyala Pogaku-1 werereleased for commercial cultivation. All thefour varieties are having high yield potentialwith superior quality.

Cultivar improvement:

● Black soils: Advanced breeding lines viz.,V-5057, RS-27 and RS-29 recordedsignificantly higher cured leaf yield, brightleaf yield and grade index over the controlSiri. The average yield increase in theselines over Siri was 19% in cured leaf, 23% inbright leaf and 20% in grade index.

● Northern light soils: Somaclones, NLCR-1-9-2-13 and NLCR-7-11-1-4, advancebreeding lines, RT3-1 and RT4-3, recordedsignificantly higher yields over Kanchan withan increase of 20-41 % cured leaf yield and41-65% grade index.

● Karnataka Light Soils: Advanced breedinglines, FCH 239 and FCH 242 recorded highestcured leaf (2600 and 2579 kg), bright leaf(1443 kg and 1716 kg) and TGE (1409 and1708 respectively).

● Irrigated natu tobacco: Advanced breedinglines, NF3-5-1 and NF3-6-2 recordedsignificantly higher cured leaf (19-59%) thancheck, Kommugudem.

● Chewing tobacco: Selection HV.2011-2 wassignificantly superior to the best checkAbirami in leaf yield with 4144 kg/ha (anincrease of 11.0%).

● Hybrid tobacco: Ten CMS hybrids showedsignificant standard heterosis over checkSiri. The heterosis varied from 18-34% forcured leaf, 14-35% for bright leaf and 17-36% for grade index.

● Disease resistance: Black shank resistancewas incorporated into released varietiesviz.,Siri, N-98 and Cy-142. Two hundred andthirty three progenies recorded resistancefor Black shank.

Germplasm resource management:

● The total available genetic resources atICAR- CTRI stand at 3369 accessions.

● Wild Nicotiana species: A total 210accessions of 56 wild Nicotiana species andtwo subspecies, 9 exotic hybrids, 5 localinterspecific hybrids and 4 amphidiploidswere maintained. Eleven non-floweringaccessions were rescued through in-vitromicro propagation.

● DUS Characterisation: Three genotypesviz., Siri, TBST-2 and unique germplasm324C were characterized for 53Distinctness, Uniformity and Stability (DUS)characteristics.

Biotechnology for tobacco improvement:

● Eleven SSR markers viz., TM51534, TM10606,TM10891, TM50469, TM1111062, TM50868,PT61653, PT10375, PT50065, PT60723 andPT53345 were found to be polymorphic forTSNA, nicotine and solanesol traits.

● Transcript analysis revealed the coregulation of four defense response genesnamely NtMEK2, PR1a, PAL1 and are impliedto be associated with the damping offdisease tolerance mechanism in Tobacco.

● The rapid evolving loci trnH–psbA (intergenicspacer region) is found to be polymorphicamong the Nicotiana accessions withrespect to amplicon length and sequence.

Development of Agro-Technologies

FCV tobacco

● Tray seedlings coupled with drip fertigationat 3, 20-25 and 40-45 DAP showed increasedcured leaf yield (16.67%) and grade index

7 ICAR - CTRI Annual Report 2015-16

(23.44%) over the practice of normalseedlings + soil application of fertilizers at10, 25-30 and 40-45 DAP + furrow irrigatoionin Northern Light Soils.

● Application of urea/ ammonium sulphate(AS) as a basal dose followed by urea or ASor urea+ AS or urea + AS+ potassium nitrateor urea + AS + calcium nitrate as top dressingwere at par in cured leaf yield and gradeindex under the conditions of NLS.

● Application of FYM + NPK or FYM + Excess N+ PK, resulted in enhanced productivitywithout false maturity.

● Application of 100 kg K2O/ha to nursery in4 splits (at sowing, 25 DAS, 35 DAS and 45DAS) recorded significantly higher drymatter production and higher number oftransplantable seedlings per unit areacompared to 25 or 50 kg/ha.

● On Karnataka Light Soils, FCV tobaccoproductivity tends to increase withincreased number of rainy days andsignificantly correlated with the sun shinehours during the crop season.

● Organic tobacco cultivation increased thebright grade production by 6-7%, reducedincidence of wilt disease as well as root knotincidence by 40-45% in organic tobacco.

● Pre plant application of Pendimethalin @325 g a.i and Alachlor @ 1000 g ai/ harecorded on par yields with that of handremoval of orobanche in black soil growntobacco.

● In Vertisols, application of FYM + NPK andremoval of orobanche produced highercured leaf yield and grade index withoutany false maturity. cv. VT-1158 performedbetter than TBST-2 without any falsematurity.

Non-FCV tobacco

● Application of 112 kg N + 112 Kg P2O5 + 112Kg K2O/ha recorded significantly highercured leaf (2331 kg/ ha) yield in Motihari

tobacco as compared to control, FYM @10t/ha.

● In burley tobacco, Set Row Plating (SRP)with 100% RDF showed significantly highercured leaf yield and N, P, K uptake.Application of 50% or 75% RDF in SRP was asgood as conventional planting with 100%RDF.

● Advanced chewing tobacco breeding lineHv.2009-3 significantly increased the firstgrade leaf yield by 10.6% and total curedleaf yield by 13% over the ruling varietyAbirami.

● Annual moringa was identified as suitableintercrop in chewing tobacco in Tamil Nadu.

Management of resource constraints forproduction efficiency and product quality

● Biomass ashes resulting from crop residueburning or wood ash from tobacco curingbarns can serve as potential liming materialand improves K-fertility of soil as evidencedfrom increased levels of K availability inbiomass amended soils.

● Application of crop residue/wood asheseither alone or in combination with SOP (50%+ 50%) on 100 kg K ha-1 equivalent basiscaused a significant increase in tobaccocured leaf yield and K uptake withoutaffecting the leaf quality. The relative yieldof tobacco with different biomass ashtreatments ranged from 88 to 98% at Hunsurand from 98 to 105% at Jeelugumilli.

● Biochar from tobacco stems is characterizedby high content of TOC (80%), N (1.23%), P(0.78%), K (3.48%) and alkaline pH (9.42).The cation exchange capacity of tobaccostem biochar and synthetic zeolite were30 and 270 Cmol (p+) kg-1, respectively.

● Silver nanoparticles synthesized from N.tabacum leaf are spherical to irregular inshape with a particle size of 10853.3 nmand a PDI value of 1.884.

● Secondary nutrients play important role on

Executive Summary

ICAR - CTRI Annual Report 2015-16 8

plant growth, metabolism and nutrientuptake under sufficient supply condition ofprimary nutrients (N, P and K) only.

● Among the nine bidi tobacco varieties,MRGTH1 showed maximum neutral volatilearoma compound. Lenolenate was themajor unsaturated fatty acid present in biditobacco.

● Identified the sensitive bands in tobaccocrop canopy spectral reflectance for leafpotassium at 747 – 862 nm, nicotine at 539-541 nm and 710 nm, reducing sugars at631-692 nm and 1901-1970 nm and forleaf total nitrogen at 350-365 nm.

Integrated Management of Biotic stresses

● In Central and Southern black soils, Leafcurl and tobacco caterpillar, were the maininsect pests in tobacco nursery and plantedcrop.

● Under Karnataka light soils condtions, fivemajor plant parasitic nematodes were foundassociated with tobacco viz., Meloidogynespp, Rotylenchulusreniformis,Helcotylenchus spp, Pratylenchus spp andTylenchus spp.

● Chlorfluazuron 5.4 EC 0.03% was foundhighly effective against Spodoptera lituraFabricius in nurseries as well as in plantedcrop of FCV tobacco. Flonicamid 50 WG andpymetrozine 50 WG @ 0.02% were foundpromising against tobacco aphid, Myzusnicotianae Blackman in FCV tobacco.Pymetrozine 50 WG @ 0.02%, flonicamid 50WG @ 0.02% and spiromesifen 240 SC @0.02% were promising against tobaccowhitefly Bemisia tabaci (Gennadius) in FCVtobacco. The residues in FCV tobaccosprayed with flonicamid 50 WG @ 0.02% andPymetrozine 50 WG @ 0.02% were less than1 mg/kg after 15 days of spray.

● Ground beetle, Mesomorphus villigerBlanchard could be managed by seedlingroot dip with Imidacloprid 70 AF @ 0.14% orapplying imidacloprid 200 SL @ 0.005% intransplant hole.

● Spraying of either pyraclostrobin + metiram@ 0.2% or carbendazim @ 0.05%

immediately after disease incidencefollowed by another spray after 10-15 daysfound effective against Frog eye spotdisease in tobacco caused by cercosporanicotianae.

● Application of fenamidone 10% + mancozeb50% @ 0.3% is a promising alternative tometalaxyl 8% + mancozeb 64% for themanagement of leaf blight disease intobacco nurseries.

● Use of Hi tech sprayer was superior till 50DAP and thereafter HPKS in terms of savingspray fluid and time.

● The bio-intensive IPM module with two rowsof maize border as barrier crop, one sprayof Verticilliumlecanii @ 3X1012 CFU/ha at50 DAP and one spray of imidacloprid @ 50ga.i./ha at 60 DAP effectively controlled thetobacco aphid

● An Integrated module comprising of ofTrichoderma viride & Paecilomyceslilacinus, Trichoderma viride & Pochaniachlalymdosporia used in conjunction withridomil and furadon in solarised nurserybeds was developed for effective controlof nematode incidence, damping off andblight diseases incidence.

● Tray seedlings enriched with T. viride (30g) + P. lilacinus (30 g) + P. chlamydosporia(30 g) increased the cured leaf yield by10.5 per cent and decreased the wilt diseaseby 51% over the check.

Exploiting tobacco for alternative uses

● Line RT 51-1 with 60 x 40 cm spacing and150:75:75 NPK kg/ha recorded highernicotine yields. HDBRG with 70 x 40 cmspacing and 150:75:75 NPK kg/ha recordedhigher solanesol (55.10 kg/ha) and proteinyield (925.7 kg/ha).

Krishi Vigyan Kendra, Kalavacharla

● A total number of 6 On-Farm testings (OFTs)and 10 Front-line Demonstrations (FLDs), 4vocational trainings and 14 trainigprogrammes were conducted during 2015-16 in agriculture, horticulture, animalhusbandry and income eneration activities.

Executive Summary

9 ICAR - CTRI Annual Report 2015-16

● Tissue culture Coccinia increased the yieldsby 4.5 tonnes/ha compared to localcultivated variety.

● Popularized Black Bengal goats in the EastGodavari district by supplying 11 units tothe farmers through ATMA

● Integrated management of snails with baitformulation of jaggery slurry + Dichlorovas+ wheat flour was effective with a mortalityof 23%.

● Vocational trainings on income generationactivities viz.,Garment making, handicraftswith palmyrah leaves, maggam embroderyand palmyrah fibre extraction, Handicraftswith weeds and post harvest value additionviz.,value addition to fruits and vegetables,fish/prawn and meat’value added productswith milk, were conducted for enhancementof family income.

● In collaboration with ATMA-East Godavaridistrict, KVK organised ‘Kisan Mela’ on09.11.2015 at KVK, Kalavacharla. A totalnumber of 300 farmers from East Godavaridistrict participated in the programme.

Krishi Vigyan Kendra, Kandukur

● Two front line demonstrations wereconducted on newly identified FCV tobaccoline, TBST-2 and also on milky mushroomproduction.

● Fodder cuttings of CO - 4 grass weresupplied to the farmers for promotingfodder production in Prakasam district.

● Fifty farmers were trained on milkymushroom production for two days.

OTHER ACHIEVEMENTS

ISO 9001:2008

● ICAR-CTRI, Rajahmundry has been grantedISO 9001:2008 certificate for the successfulimplementation of its quality managementsystem with regard to “Basic, Strategic andApplied Research on Tobacco Crop” on25.01.2016.

National Seminar on Crop Diversification

● The ICAR –CTRI has organised DAC&FWsponsored National Seminar on “CropDiversification” at Rajahmundry on18.01.2016. Scientists, Farmers and Industryrepresentatives participated in the seminarand deliberated on a myriad issues relatingto crop diversification in tobacco growingareas. On the whole the National Seminarserved as a platform for exchange ofinformation and experiences on variousissues relating to crop diversification intobacco growing regions.

Tribal Sub-Plan:

● Tribal Sub-Plan ofICAR-CTRI wasimplemented in Manchulavarigudem, atribal hamlet of Seethappagudem grampanchayat, West Godavari district, AndhraPradesh during 2015-16. Five interventionsviz., Supply of foundation seed of highyielding maize hybrid (DH-117), fertilizers,farm equipment (weighing balances,Improved sprayers), construction ofpermanent grain storage bins wereimplemented for a total number of 38 farmfamilies.

● Tribal Sub-Plan of AINP on Tobacco wasimplemented by AINPT Centre at RARS,ANGRAU, Nandyal. Interventions includingsupply of critical inputs (fertilizers,pesticides and improved varieties) forproductivity improvement, trainingprogramme for increasing productivity inmillets and pulses and supply of relevantliterature were implemented for 40 tribalfarmers of Sugalimetta village of PanyamMandal in Anantapur, Andhra Pradesh.

AP-TECH 2015

● Andhra Pradesh Technology Developmentand Promotion Centre (AP-TDC), Hyderabadwith the technical collaboration of ICAR-CTRI organised an Agri-Exhibition (AP-TEC2015) at Rajahmundry from 05.06.2015 to07.06.2015.

Mera Gaon Mera Gaurav

● Mera Gaon Mera Gaurav (My Village MyPride) was initiated at ICAR-CTRI,

Executive Summary

ICAR - CTRI Annual Report 2015-16 10

Rajahmundry in the month of November,2015. The scientists have adopted villagesand completed the base line survey of thevillage. The regular farmers’ advisoryservices are being provided from time totime.

World Soil Day

● World Soil Day was celebrated on 05.12.2015at CTRI Research Station, Hunsur,Karnataka, CTRI-KVK, Kalavcharla (EastGodavari dt.), A.P and CTRI-KVK, Kandukur(Prakasam dt), A.P. In this occasion, 606 SoilHealth Cards were issued to farmers ofAndhra Pradesh and Karnataka.

Jai Kisan Jai Vigyan Week

● Jai Kisan Jai Vigyan week was implementedby ICAR-CTRI at Rajahmundry from 23-29December, 2015 for promoting the use of

science for the welfare of farmers.Innovative farmers and farm women werefelicitated in recognition of theircontribution to agriculture and alliedsectors.

Swachh Bharat Programme

● Swachh Bharat Programmes wereimplemented with a focus on conservationof natural resource management (water, soiland energy).

Awards & Recognitions

● Dr. D. Damodar Reddy, Director, ICAR-CTRI,Dr. K. Suman Kalyani, Principal Scientist,Dr. H. Ravisankar have been honorouredwith prestigious awards/ recognitions fortheir outstanding contribution in Science &Technology.

Executive Summary

11 ICAR - CTRI Annual Report 2015-16

IntroductionThe Central Tobacco Research Institute

(CTRI), established in 1947, is a constituentInstitute of the Indian Council of AgriculturalResearch, New Delhi and has the exclusivemandate to undertake basic, strategic andapplied research on various types of tobaccogrown in India with special emphasis onexportable types of tobacco. Six regionalstations situated at Guntur, Kandukur andJeelugumilli in Andhra Pradesh; Vedasandur inTamil Nadu; Hunsur in Karnataka; and Dinhatain West Bengal and a Research Centre atKalavacharla in Andhra Pradesh are catering tothe requirements of tobacco farmers in differentagroclimatic zones by developing improvedvarieties and site specific agro technologies. TheAll India Network Project on Tobacco with itsmain centres and sub-centres across the countryis carrying out multi-locational trials on varioustypes of tobacco.

The research perspectives concerningtobacco are undergoing a continuous changeowing to emerging issues such as naturalresource degradation, climate change, bioticand abiotic stresses, energy saving and alternatesources of energy and others related to society,trade and Government policies at national andinternational level. The challenges confrontingtobacco and the tobacco researchers are nowmore varied and complex than ever before andcall for a paradigm shift in our researchapproach to make tobacco enterpriseremunerative and profitable to the farmingcommunity. Against this background, theresearch programmes were reoriented at thebeginning of XII plan. The mandate and thereoriented research programmes of the instituteare furnished hereunder.

Vision

Provide vibrant research back-up forIndian tobacco to be less harmful,remunerative and globally competitive inthe changing milieu of national andinternational policy regimes.

Mission

Developing environmentally sustainableagro-technologies for production

efficiency, product quality and diversifieduses of tobacco.

MANDATE

✤ To conduct research on different types oftobacco, with greater emphasis onexportable types, on all phases ofproduction management with a view ofattaining economic advantage / benefit tothe tobacco growers through improvementin quality and quantity of tobacco.

✤ To collect tobacco germplasm from worldover and to maintain and operate tobaccogenetic resources which will be madeavailable to scientists and National /International Institutions.

✤ To conduct research on economically viableand sustainable cropping systemsalternative to tobacco.

✤ To conduct research on diversified uses oftobacco and development of value-addedproducts viz., phytochemicals.

✤ To produce and distribute quality seeds ofnotified varieties of tobacco.

✤ To publish and disseminate research findingsand recommendations of latest technologyfor the benefit of the tobacco growers,scientific community, policy makers anddevelopment agencies.

Quality policy:

ICAR-CTRI shall l focus on:

The ICAR- CTRI shall strive to provide vibrantresearch back-up for Indian tobacco to be lessharmful, remunerative and globally competitivein the changing milieu of national andInternational policy regimes.

WITH FOCUS ON

✤ Ensuring production of “quality tobacco”with reduced levels harmful constituents

✤ Enhancing farm returns through innovativeinterventions for sustainable resource useand production efficiency

✤ Exploiting and effective use of green energysources for FCV tobacco curing

ICAR - CTRI Annual Report 2015-16 12

✤ Exploiting tobacco for diversified uses(Phytochemicals and Value addition)

✤ Effective technology transfer/consultancyservices to address the stakeholders needs.

RESEARCH PROGRAMMES

I. Tobacco cultivar improvement

(A) Developing tobacco varieties / hybridspossessing higher leaf yield and resistanceto biotic and abiotic stresses to stabilizeproductivity

(B) Tailoring of tobacco plant type foroptimizing the seed yield andphytochemicals

(C) Production and distribution of foundationseed of ruling tobacco varieties

(D) Germplasm resource management

(E) Biotechnology for tobacco improvement

II. Development of agro-technology forsustainable tobacco production andstrengthening TOT

(A) Optimization of water and nutrient usefor productivity enhancement of differenttobacco types

(B) Evolving site-specific cultural managementpractices in different agro-ecological sub-regions

(C) Post-harvest product management (PHPM)

(D) Analysis of socio-economics for stratificationand to formulate appropriate strategies

(E) Technology outreach activities

(F) Technology assessment

III. Identification of alternative crops andexploiting tobacco for alternative uses

(A) Alternative crops to FCV and non-FCVtobacco in different agro-ecological sub-regions

(B) Agro-techniques for higher biomass and seedyield

(C) Identification of potential phytochemicals

IV. Management of resource constraints forproduction efficiency andproduct quality

(A) Evaluation of soil fertility, water quality andplant nutrition constraints for tobacco andtheir management

(B) Soil quality and nutrient-use-efficiency inrelation to input management

(C) Characterization of soil biota and use ofbiofertilizers

(D) Evaluation of tobacco leaf and productquality

V. Integrated management of biotic stresses

(A) Screening for host plant resistance to insectpests and diseases

(B) Development of IPM technology

(C) Evaluation of new molecules andformulations of pesticides for bio-efficacy

(D) Monitoring of insect pests and diseases

(E) Weather forecasting and its influence onincidence of pests and diseases

Introduction

13 ICAR - CTRI Annual Report 2015-16

STAFF POSITION AND FINANCIAL STATEMENT

FINANCIAL STATEMENT FOR THE YEAR 2015-16

Head of Accounts Rupees in lakhs

Budget Grant Expenditure

Non-Plan 2147.70 2147.62Plan 192.00 192.00KVK, Kalavacharla 146.00 136.49KVK, Kandukur 8.39 8.34AINPT 392.00 392.00Pension & Retirement Benefits 1500.00 1500.02Personal Loans & Advances 15.00 15.00Recurring deposits Schemes 19.97 20.35Revolving Fund Scheme 82.67 43.86Internal Resource Generation 47.44 4.64Revenue Receipts 165.36

STAFF POSITION AS ON 31.03.2016

Sl. Category SanctionedNo. Strength In Position Vacancies

1. Scientific 56+1* 26+1* 30

2. Technical 158 107 51

3. Administration 71 45 26

4. Skilled Supporting Staff 160 119 41

* RMP position

Introduction

ICAR - CTRI Annual Report 2015-16 14

ORGANOGRAM

Shimoga Pusa

AINPTSub-Centres

Berhampur Dinhata

Guntur Araul

Hunsur Nandyal

Nipani

Jeelugumilli Kandukur

Vedasandur Ladol

AINPTVoluntary Centres

Rajahmundry Anand

Research AdvisoryCommittee

QuinquennialReview Team

ResearchDivisions

AINPTMain Centres KVK

DIRECTOR,ICAR-CTRI

Secretary, DARE &Director - General, ICAR, New Delhi

Dy. Director - General (Crop Science),ICAR, New Delhi

Institute ManagementCommittee

ResearchStations

CropImprovement

CropProduction

CropProtection

Crop Chemistry& Soil Science

Guntur

Kandukur

Jeelugumilli

Hunsur

Vedasandur

Dinhata

BTRC,Kalavacharla

Administration

Audit &Accounts

PMECell

AKMU &LDS

MSU

KalavacharlaE.G. Dist., A.P.

KandukurPrakasamDist., A.P.

Introduction

15 ICAR - CTRI Annual Report 2015-16

ICAR - CTRI Annual Report 2015-16 16

I. Tobacco Cultivar Development

I. (A) Developing tobacco varieties with higherleaf yield and quality (CTRI,Rajahmundry)

Evolving superior varieties of FCV tobaccothrough hybridization:P.V.Venugopala Rao

Evaluation of advanced breeding lines for yieldand quality

A replicated yield trial was conducted forthe first Year with ten advanced breeding linesviz., V-5047, V-5050, V-5051, V-5057, V-5058,V-5059, V-5060, V-5061, V-5063 and V-5068 andtwo controls viz., VT-1158 and Siri.

Among the lines evaluated, V-5057recorded significantly superior green leaf yield(14453 kg/ha) by 10 %, cured leaf yield (2170kg/ha) by 12%, bright leaf yield (1374 kg/ha) by12 % and grade index (1808) by 15 % over Siri(13077, 1936, 1231 and 1568, respectively).

Chemical quality parameters

The nicotine (%) ranged from 1.37 to 3.20and reducing sugars (%) ranged from 9.81 to19.84 in the entries.

Based on the performance during 2013-14,V-5057 is only better performer among theentries evaluated with 10 per cent improvementin green leaf, 12 per cent improvement in curedleaf and bright leaf and 15 per centimprovement in grade index compared to thebetter check Siri.

Preliminary evaluation of advanced breedinglines:

Advanced breeding lines V-5083 to V-5126(43) were evaluated in a row trial along withthe control Siri and VT-1158. Ten selections viz.,V-5083, V-5089, V-5096, V-5100,V-5111, V-5113,V-5119,V-5120, V-5121 and V-5124 a made basedon the higher yield over Siri during 2014-15.These selections will be evaluated in areplicated trial along with Siri and VT-1158during 2015-16. Another set of 58 selectionswere raised and sixty selections were made for

evaluating in a row trial during the ensuingseason. From a F2 population of 1380 plants 82selections were made for further study.

Evaluation of advanced breeding lines for yieldand quality (CTRI, Rajahmundry)K. Sarala, K. Prabhakar Rao, T.G.K. Murthy,P.V. Venugopala Rao and S.K. Dam

Thirteen advanced breeding lines weretested in a replicated trial for second year alongwith two controls, VT 1158 and Siri

Significant differences were observedamong the tested lines for green leaf, curedleaf and bright leaf. Lines RS 27 and RS 29recorded significantly higher green (17500 &18090 kg/ha) and cured leaf yields (2800 & 2780kg/ha) and bright leaf (1286 &1298kg/ha), andhigher grade index (2086 & 2126 kg/ha) thanthe better control, Siri. The range of yieldincrease in these lines over Siri(c) is 17% in greenleaf, 19% in cured leaf, 23% in bright leaf and20% in grade index).

Chemical quality characteristics of breedinglines viz. nicotine range from 1.11% in RS-26,2.64% in Rs-32 and reducing sugars from 8.67%in RS-29 and 15.99% in RS-22

17 ICAR - CTRI Annual Report 2015-16

Seasons and seasons x entries interaction weresignificant for yield parameters. NLCR-1-9-2-13 & NLCR -9-2 recorded significantly higheryields of all types than kanchan in all theseasons.

Based on the overall performance, entries,NLCR-1-9-2-13, NLCR-7-11-1-4, NLCR-BT1-P2,NLCR-BT2-P9, VLCR-12-15-14-5, NLCR-9-2 andPasidi Kanchan P2 are to be advanced formultilocation testing under AINPT.

Nicotine at ‘X’ position found to be in rangefrom 1.23-3.18% and ‘L’ position from 1.15-3.33%. Reducing sugars at ‘X’ position found tobe in range from 11.42 to 20.64% and ‘L’ positionfrom 4.91-16.16%.

Six entries were tested in a bulk trial alongwith Kanchan. All the entries viz., NLCR (Tobios-6), NLCR-7(k) (Tobios-2), NLCR-4 (Tobios-7),NLCR-7 (FCJ -3), NLCR-10(Tobios-3) and NM (FCJ-

Screening of breeding lines in row trial

Out of 90 breeding lines assessed for yieldunder row trial, 37 lines found to have desirablemorphology and yield. One hundred and fiftyadvanced breeding lines were raised and seedcollected for maintenance. One hundred andtwenty four lines including breeding lines,germplasm lines and F2s were screened for TMVresistance and resistant plants selfed and selfedseed collected.

Twenty promising Kanchan and 12 VT-1158somaclones were inoculated with black shankunder artificial conditions and seed collectedfrom resistant plants.

Evaluation of breeding lines (CTRI RS,Jeelugumilli)

Twelve somaclones and two breeding lineswere tested for third year in a replicated trialalong with Kanchan(C). NLCR-1-9-2-13, NLCR-7-11-1-4, NLCM-1-5(a)-2, NLCR-BT1-P2, NLCR-BT2-P9, VLCR-12-15-14-5, NLCR-9-2 and PasidiKanchan P2 recorded significantly higher yieldsthan Kanchan. The green leaf yield in these linesranged from 16653 to 20111 kg/ha, cured leafyield from 2894 to 3406 kg/ha and grade indexfrom 2107to 2463 kg/ha; an increase of 22-48%,20-41 % and 41- 65%, respectively, over control,Kanchan. Entry, NLCR-8-15-9 and NLCR-10-7-2-1 recorded significant cured leaf yields (2821and 2892 kg/ha, respectively)

Pooled analysis was done for leaf yields,Leaf yields found to be significant among thelines. Clones, NLCR-1-9-2-13, NLCR-7-11-1-4,NLCR-BT2-P9 and NLCR-9-2 lines recorded 10-27% increase in green leaf yield, 8-26% in curedleaf yield and 15-37% in grade index thanKanchan.

Entry / Line Cured leaf Grade index

NLCR-1-9-2-13 3406 2463NLCR-7-11-1-4 2894 2180NLCM-1-5(a)-2 3092 2374NLCR-BT1-P2 3265 2087NLCR-BT2-P9 3172 2274VLCR-12-15-14-5 3334 2015NLCR-9-2 3172 2286Pasidi Kanchan P2 3376 2107Kanchan(C) 2410 1497

Tobacco Cultivar Development

ICAR - CTRI Annual Report 2015-16 18

Tobacco Cultivar Development

4) recorded higher leaf yields than Kanchan. Theincrease in cured leaf yields in these lines rangedfrom 15-73% and grade index from 17-87%.

Chemical quality characteristics viz.Nicotine at ‘X’ position ranged from 1.60-3.01%and ‘L’ position from 2.21-3.22%. Reducingsugars at ‘X’ position ranged from 11.17 to17.57% and ‘L’ position from 9.07-18.34%.

Evolving FCV tobacco varieties having highyield and better quality suitable for NLS areaof Andhra PradeshT.G.K. Murthy

I. Generation advancement & selection:

Thirty F8 progenies of crosses involvingKanchan as one of the parents were raised and26 of the lines were found to be TMV resistant.Single plant selections showing plant typesuitable to NLS besides having high leaf number(30-45 per plant) and/or resistance to TMV wereselfed for further testing.

II. Preliminary evaluation of advancedbreeding lines

A progeny-row trial was conducted with 146advanced breeding lines along with the checkvariety Kanchan to identify selections suitableto NLS area. The lines varied for importantmorphological and agronomical traits. Fifteenlines with high yield potential (CLY 3300-4500kg/ha against 2590 kg in Kanchan) and desirableleaf quality were identified. Seven of the lineswere identified as uniformly resistant to TMVon artificial inoculation. Single plant selectionsshowing good plant type and leaf characteristicssuitable for NLS besides high yield potential,were advanced for further evaluation.

In addition to high yielding selections, foursemi-dwarf selections with compact plant typeand very short internodes, suitable for closespacing were also identified and advanced forfurther study.

III. Replicated yield trials

1. Trial RYT-15 (3rd year)

A set of 13 green cast advanced breedinglines were evaluated along with check Kanchan

for leaf yield and quality traits in a RBD withthree replications for the third year insuccession. Lines RT9-1 and RT29-1 showedresistance to TMV also.

Seven lines (RT3-1, RT4-3, RT9-1, RT29-1,RT29-2 and RT33-1& RT49-1) showedsignificantly higher cured leaf yield and gradeindex than check, Kanchan. The increase was13-47% for cured leaf yield and 20-42% for gradeindex. Cured leaf colour, size and body in thetest entries viz., RT Nos.9-1, 10-1, 33-1, 48-1,and 49-1 were comparable with that of Kanchan.

Combined statistical analysis of the yielddata over last three seasons for identifyingpromising lines revealed that seven lines (RT3-1, RT4-3, RT9-1, RT10-1, RT29-1, RT29-2 andRT33-1) showed significantly higher cured leafyield and grade index than check, Kanchan. Theincrease in these lines was 11 to 45% for curedleaf yield and 9 to 16 % for grade index,respectively over Kanchan.

Seasonal differences were significant andyield levels during 2014-15 were higher than theother two seasons. Season x entry interactionwas also significant for all the four yield traits.

Chemical quality of leaf: The nicotine andreducing sugars in the lines were withinacceptable limits.

Based on overall performance seven linesRT3-1, RT4-3, RT9-1, RT10-1, RT29-1, RT29-2,and RT33-1 are proposed for further studies.

RT 3-1

19 ICAR - CTRI Annual Report 2015-16

2. Trial RYT-16 (3rdyear)

In another replicated trial, 13 medium/green cast advanced breeding lines wereevaluated along with check Kanchan for leafyield and quality in a RBD with threereplications. Plant and leaf types were good inRT Nos. 55-1, 57-1, 96-1, 81-1, 108-1, 66-1 and91-1. Lines RT91-1, RT92-1 and RT96-1 andRT108-1 showed resistance to TMV.

Five lines (RT66-1, RT91-1, RT92-1, RT96-1and RT108-1) showed significantly higher curedleaf yield and grade index than check, Kanchan.The increase was 22-47% for cured leaf yieldand 18-49% for grade index.

Cured leaf colour, size and body in the testentries RT Nos. 55-1, 57-1, 91-1, 92-1, 94-1,104-1 and 108-1 were comparable with that ofKanchan. Nicotine and reducing sugars werewithin admissible limits.

for all the four yield traits. The increase inthese lines was 13 to 44% for green leaf yield,17 to 46% for cured leaf yield, and 20 to 51% forgrade index, respectively over Kanchan.

Seasonal differences were significant andyield levels during 2014-15 were higher than theother two seasons. Based on overallperformance the entries RT66-1, RT91-1, RT92-1, RT94-1, RT96-1 and RT108-1 are proposed forfurther studies.

3. Trial RYT-17 (2nd year)

The third replicated yield trial wasconducted for the second year in succession with13 green cast advanced breeding lines alongwith check Kanchan for leaf yield and quality ina RBD with three replications. Plant and leaftypes were good in all the test entries likeKanchan.

Resistance to TMV was observed in theentries RT110-2, 113-2 and 114-3 while six othersshowed segregation for the trait.

Eight lines (RT54-1, RT55-1, RT56-1, 109-1,RT113-2, RT114-3, RT121-1 and RT123-1) showedsignificantly higher cured leaf yield and gradeindex than check, Kanchan. The increase was16 to 45% for green leaf yield, 17-46% for curedleaf and 20-66% grade index.

Cured leaf colour, size and body in the alltest entries were comparable with that ofKanchan. Nicotine and reducing sugars werewithin admissible limits.

4. Trial RYT-18 (2ndyear)

In the 4threplicated trial conducted,medium/ green cast advanced breeding lineswere evaluated for the second year along withcheck Kanchan for leaf yield and quality in aRBD with three replications. Plant and leaf typeswere good in all the test entries, like Kanchan.

Analysis of data indicated significantdifferences for cured leaf yields and grade indexamong entries. Four lines viz., RT 121-1,129-3,132-1 and RT135-1 showed significantly highercured leaf yield and grade index than Kanchan.The increase was 11 to 31% for cured leaf yield

Combined statistical analysis of the yielddata over three seasons (2012-15) for identifyingpromising lines revealed that six advanced crossderivatives viz., RT66-1, RT91-1, RT92-1, RT94-1, RT96-1 and RT108-1exhibited significantlysuperior performance over best check, Kanchan

Tobacco Cultivar Development

ICAR - CTRI Annual Report 2015-16 20

Tobacco Cultivar Development

and 17 to 46% for grade index, respectively inthe two promising lines.

Cured leaf colour, size and body in the testentries were good and comparable withKanchan. Nicotine and reducing sugars werewithin admissible limits.

5. Trial RYT-19 (1st year)

In a new replicated trial with 11 medium/green cast advanced breeding lines wereevaluated along with check Kanchan for leafyield and quality in RBD. Plant and leaf typeswere good in all the test entries, like Kanchanand initial vigour was observed in the entriesRT Nos. 94-1, 108-2 and 118-1. Resistance toTMV was observed under artificial inoculationin two entries, RT93-1 and RT94-1.

Analysis of data indicated significantdifferences for cured leaf yields and grade indexamong entries. Four lines RT Nos. 126-1, 128-1,135-1 and RT137-2 showed significantly higherleaf yield and grade index than Kanchan. Theincrease was 18 to 39 % for green leaf yield, 14to 40% for cured leaf yield and 15 to 36% forgrade index, respectively. Cured leaf colour, sizeand body in all the test entries were comparablewith Kanchan.

IV. Bulk assessment trial and on-farm trials:

Bulk trial: One bulk assessment trial wasconducted with 8 entries viz., FCJ-1, FCJ-4,NLST-2, NLST-3, NLST-4, NLST-5, NLST-6 andKanchan (check). All the lines were superior toKanchan in Station and AINPT multilocationtrials.

On-farm trial: Lines NLST-3, NLST-4 and NLST-5 were evaluated in on-farm testing atNutiramannapalem village in NLS for obtainingfarmer’s acceptance during the year. Line NLST-4 (2104 kg/ha CLY) showed 9.5% improvementover Kanchan (1922 kg) and was opined assuitable for further testing.

Developing new varieties of irrigated natutobacco for Andhra PradeshT.G.K. Murthy

1. Bulk evaluation

Nine advanced breeding lines, identified assuperior to checks in previous bulk assessmenttrials, were grown in progeny bulks along withcheck, Kommugudem. Among all the lines, 45-90 recorded about 16% higher total cured leafyield than Kommugudem.

2. Evaluation of promising lines in row trial

Thirteen advanced breeding lines derivedfrom two crosses viz., Singarajupalem xKommugudem and Kommugudem x 45-90 wereevaluated along with check variety,Kommugudem for leaf yield potential andphysical leaf quality in a progeny row trial. Allthe lines showed desirable “Katta” characterssuitable for cultivation as irrigated Natu. Mostof the lines showed higher leaf yield potentialthan check, Kommugudem

3. Evaluation of promising lines in replicatedtrial - 2 (2nd year):

Fifteen green cast Natu advanced breedinglines with high yield potential were evaluatedin a RBD along with check Kommugudem for leafyield and quality. Plant type and leaf colour,body and size were good in all the entries.

Analysis of data indicated significantdifferences for cured leaf yields among entries.Three test entries viz., NF4-16, NF4-22 & NF4-showed significantly higher (18 to 36%) curedleaf yield than Kommugudem.

4. Evaluation of promising lines in replicatedtrial - I (3rd year):

Fifteen green cast Natu type advancedbreeding lines with high yield potential and /NLST-4

21 ICAR - CTRI Annual Report 2015-16

or TMV resistance, were evaluated in RBD alongwith check Kommugudem for leaf yield andquality. Plant type and leaf colour, body andsize were good in most of the test entries.

Analysis of data indicated significantdifferences for cured leaf yields among entries.Entries NF3-5-1, NF3-6-2,NF3-8-1, NF3-10-2,NF3-11-1, NF3-12-1, NF3-12-2, NF3-15-1, NF3-20-2, NF3-15-2 and PVM14-1 recordedsignificantly higher cured leaf (19-59%) thancheck, Kommugudem (2026 kg/ha).

Based on aroma, colour, leaf size, leafaroma and flavour, leaf blemish and weight, thelines viz., NF3-5-1, NF3-6-2, NF3-12-2 and NF3-15-1 are found suitable for irrigated conditions.

selections were made based on the plantcharacters and these selections will beevaluated further during 2015-16.

Maintenance of Burley germplasm:

One hundred and twenty one germplasmlines ByGP-1 to ByGP-121 were planted andselected plants are selfed and seed collectedfor further maintenance.

Incorporation of Male sterility (CMS) in burleyVarieties:

The BC9 crosses involving the male sterilehybrids BRK-1, BRK-2, TN-97, NCBH-127 and NC-3 were raised and back crossed with therespective male fertile recurrent parent viz.,Banket A1, Burley-21, VA-510, Banket-127 andseed collected.

Evaluation of advanced breeding lines

Ten selections made viz., YB-26 to YB-35were evaluated for first year along with checksBanketA1(C), Burley-21(C), Swetha(C) and YB-4in a replicated trial during 2014-15. The lineYB-27 showed 34 % increase in the yield (2217kg/ha) over control Banket A1 (1649 kg/ha) andthe improvement was 34 per cent followed byYB-33 (1916 kg/ha) with an improvement of 16%over control Banket A1. Nicotine in the entriestested ranged from 0.72 to 2.49 % and reducingsugars ranged from 0.08 to 1.68 % .

Development of high yielding TMV resistantFCV varieties suitable for cultivation in AndhraPradesh (CTRI RS, Guntur)T.G.K.Murthy and P. Venkateswarulu

Eight FCV tobacco lines were evaluated forassessing their performance in respect of yield,quality and disease resistance. Compared tocheck Siri, significant difference in all the yieldparameters was observed in three lines, viz.,RT 42-1, RT 44-1 and RT 130-1. A cured leaf yieldof 2,559 kg and bright leaf of 1,791 kg/ha wasrecorded in RT 42-1 followed by RT 44-1 with17,175, 2,532 and 1,799 kg/ha of green, curedand bright leaf, respectively. RT 42-1 and RT44-1 showed 26 and 25% increase in the curedleaf yield over the standard check, Siri.

NF 3-5-1

4. Generation advancement and selection: F6generation of the crosses viz., Singarajupalemx Kommugudem and Kommugudem x 45-90 wasgrown and 28 single plant selections withdesirable plant type (less height, closephyllotaxy, Katta type leaf shape, less internode length, dark green foliage and more leafnumber were selected for further studies.

5. TMV resistant irrigated Natu tobacco

Also, 8 Natu type derivatives (F9) of crossPyruvithanam x JMR were grown in progeny rowsand three lines with TMV resistance and suitableto irrigated conditions were selected.

Evaluation of advanced burley breeding linesfor productivity and qualityP.V.Venugopala Rao and T.G.K.Murthy

Evaluation of segregating material

Fifty one selections made during 2013-14were planted along with control BA1 and 95

Tobacco Cultivar Development

ICAR - CTRI Annual Report 2015-16 22

In all the entries tested, nicotine, reducingsugars and chlorides were in acceptable limits.

Pest incidence was also recorded in all thevarieties. Tobacco aphid was not noticed in anyof the varieties. Leaf curl (whitefly) incidencewas above threshold level and varies from 5.33to 9.66%. TMV incidence was low and varies from0 to 2.66%.

Another replicated trial conducted with 6advanced lines and three checks to evaluatetheir yield potential. Only two varieties, viz.,T-61 and T-63 were significantly superior to allthree checks. Among all the treatments, line T-61 was superior with cured leaf of 2,540 kg,bright leaf of 1,905 kg and grade index of 2,175kg/ha followed by T-63 with 16,791, 2,472, 1,730and 2.080 kg/ha. In case of cured leaf, anincrease of 20 and 17% was recorded in T-61and T-63, respectively over the standard check,Siri.

Chemical analysis of cured leaf indicatedthat nicotine, reducing sugars and chlorideswere at permissible limits.

Breeding FCV tobacco varieties for yield andquality characters under SLS conditions (CTRIRS, Kandukur).A.R. Panda, K.C. Chenchaiah, P.V. VenugopalaRao, T.G.K. Murty and A.V.S.R. Swamy

A. Three hundred seventeen (317) accessionsof FCV Tobacco germplasm were maintainedat CTRI RS, Kandukur.

A. Five single plants selections from the F4single plant progeny rows of Siri X 15-2 (2) andSiri X 10-1 (3) crosses with aphid resistant lineswere made.

B. Five single plants selections from the F4single plant progeny rows of Siri X 155-2 (3)and Siri X 113-1 (2) crosses with caterpillarresistant lines were made.

C. F2 generation of Siri X 47-1 (10), Siri X 62-2(10) and Siri X 151-2 (5) were grown and 25single plants were selected.

Development and evaluation of advancedbreeding lines suitable to Karnataka Light Soilregion. (CTRI RS, Hunsur)C. Nanda, M. Mahadevaswamy, S.Ramakrishnan

Eight advanced breeding lines developedfrom the crosses involving Kanchan and Rathnawith Coker 371G and NC 89 were evaluated inreplicated trial in RBD along with the checks.Analysis of data indicated that none of theentries showed any statistically significantsuperiority over check Kanchan. The entry FCH239 recorded highest green leaf yield (18693kg),cured leaf (2600kg), bright leaf (1443 kg) andTGE (1716) followed by FCH 242 (18655 kg greenleaf, 2579 kg cured leaf, 1409 kg bright leafand 1708 TGE) . The RKI of the entries was 2.1– 3.1.The leaf chemical quality parameters ofthe lines assessed are in acceptable range.

Seed production of KLSH10 and its parentswas carried out for further evaluation of thehybrid in current season.

Exploitation of heterosis and hybrid vigour forimproving tobacco yield and quality (CTRI RS,Vedasandur)Studies on heterosis in chewing tobacco (N.tabacum L.)M.Kumaresan

Out of ten F8 populations of promisingselections evaluated in a replicated yield trialalong with Bhagyalakshmi and Abirami ascontrols, the selections HV.2011-2, HV.2011-9and HV.2011-7 were significantly superior tothe control Bhagyalakshmi recording 3086(25.0%), 2963 (20.0%) and 2914 (18.0%) kg/hawhole leaf yield and 3901(19.2%), 3741(14.2%)and 3679(12.4%) kg/ha total leaf, respectively.None of the selections were significantlysuperior to the best check Abirami.

T-61 T-30

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23 ICAR - CTRI Annual Report 2015-16

Based on pooled analysis for last threeyears, only the selection HV.2011-2 wassignificantly superior to the best check Abiramiin total leaf yield recording 4144 kg/ha anincrease of 11.0% percent and the same waspromoted to the pre-release bulk evaluation infarmers’ field along with the control Abirami inthe coming season.

Pedigree Selection in chewing tobacco(N.tabacum L.) population with a broadgenetic baseM.Kumaresan

Two promising selections viz., HV.2009-3and HV.2009-5 derived from broad basedpopulations grown in pre-release bulk trial atCTRI Research Station, Vedasandur farm as wellas in four out station centres showed that boththe broad based selections performed well atCTRI-Research Station farm, Vedasandurrecording cured leaf yield of 3634 and 3585 kg/ha respectively an increase of 13.3 and 11.5 %increase over the control Abirami (3214 kg/ha).At out station centres also, the broad basedselections HV.2009-3 and HV.2009-5 performedwell recording mean cured leaf yield of 3930and 3755 kg/ha an increase of 9.7 and 4.8 %respectively over the control Abirami.

The overall mean performance of last threeyears of broad based selections (HV.2009-3 andHV.2009-5) at CTRI-Research Station,Vedasandur also revealed that both theselections were superior to the control Abiramirecording 3473 and 3337 kg /ha cured leaf yieldrespectively an increase of 11.5 and 7.1 percent.

HV-2011-2

At out station centres also they exhibitedsuperior performance registering 4121 and 3781kg/ha cured leaf yield respectively an increaseof 12.1 and 2.8 percent over Abirami.

The chemical analysis data of broad basedselections HV.2009-3 and HV.2009-5 were in theacceptable range.

Based on overall performance, it is proposedto submit variety release proposal for the broadbased selection HV.2009-3 for its identificationin the IRC meetings.

Development of hybrid tobacco

Developing hybrid tobacco suitable forTraditional black soils of Andhra Pradesh (CTRI,Rajahmundry).T.G.K. Murthy, P.V. Venugopala Rao and K.Sarala

During the year, two replicated yield trialswere conducted, one with CMS and fertilehybrids.

1. Replicated yield trial with CMShybrids

Trial Br 7.13:

Twelve CMS hybrids were produced bycrossing CMS and male parents that wereidentified as better combiners.The 12 CMShybrids were evaluated for leaf yield potentialand leaf quality along with checks Siri and VT1158 in a RBD. In general, initial vigour andplant growth was more in the CMS hybrids.

Sl. Entry Cured leaf Grade IndexNo.

1. CMSH-134 2800 23072. CMSH-135 2679 21883. CMSH-137 2517 20314. CMSH-143 2736 21885. CMSH-144 2641 21976. SIRI(c) 2088 16877. VT 1158(c) 2071 1701

Superior CMS Hybrids

Significant differences for all the yieldcharacteristics among entries are observed.Tenof the 12 hybrids showed significant standardheterosis over variety Siri. The heterosis varied

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from 17-35% for green leaf yield, 18-34% forcured leaf, 14-35% for bright leaf and 17-36%for grade index, among the hybrids.

Colour of the cured leaf in all the hybridswas better than or comparable with checks, Siriand VT 1158. Leaf size was more in the hybridCMSH-144, while leaf body was very good inhybrids CMSH nos. 133, 134, 136, 139, 140, 141,143 and 144.

The nicotine and reducing sugar levels werewithin the admissible limits in the identifiedbetter performing hybrids (nicotine 0.90 – 1.60%,reducing sugars 16.52 – 21.10%).

2. Maintenance of CMS lines: A total of 67 CMSlines with varying cytoplasm sources weremaintained.

All the lines were crossed with respectivemaintainer lines for further maintenance anduse.

Three CMS lines were shared with AINPTcentre at Shimoga.

Four crosses viz., MS-58 x HDBRG, MS-58 xVT-1158, MS-58 x A-145 and MS58 x TI-163 (allin BC8) were made to develop CMS parental lineswith high biomass potential.

Developing hybrid FCV tobacco suitable forNLS area of Andhra Pradesh (CTRI RS,Jeelugumilli)T.G.K. Murthy, P.V. Venugopala Rao and K.Sarala

1. Maintenance of CMS lines

Sixteen CMS lines in genetic background ofruling variety, Kanchan and other improved lineswere maintained and back crossed to recurrentparent. Fifteen lines are stabilized and leaflevels were recorded in all the CMS parents alongwith check cultivar, Kanchan. Leaf yield andquality in four lines viz., CMS-1, CMS-2, CMS-4,CMS-8 & CMS-13 were comparable or higher thanparent Kanchan.

2. Replicated yield trial with CMS hybrids:

Thirteen CMS hybrids, produced fromcrosses involving identified promising CMS linesand high yielding breeding lines were evaluatedalong with the check, Kanchan in a RBD for the

second year. Data on yield of green leaf, curedleaf and grade index length were recorded.

Two hybrids, MSH-4 & MSH-5 showedsignificant standard heterosis for all the threeyield traits over check, Kanchan with 34 & 43 %increase in green leaf yield, 31 & 37% in curedleaf and 28 & 27% increase in grade index.

Exploitation of heterosis and hybrid vigour forimproving tobacco yield and quality

Evaluation of FCV tobacco lines for yield andquality under SLS conditionsA.R. Panda

Three breeding lines, FC-1 a dwarf variantof Siri with smaller internodes identified fromthe bulk population of Siri, FC-2 from the crossesof Hema X NC 3150 and FC-3 from Candle X Hemaalong with seven advance breeding lines wereevaluated for the second year, to test theirsuperiority over the check varieties.

Interspecific hybridisation for tobaccoimprovement.

Incorporation of aphid resistance from N.gossei. N. repanda, N x. umbratica-nesophilaand N x benthamiana –repanda (CTRI,Rajahmundry)T.G.K. Murthy, U. Sreedhar and K. Siva Raju

1. Maintenance of interspecific crossderivatives

A total of 73 stabilized aphid andcaterpillar resistant/ tolerant advanced linesand those having high yield potential, derivedfrom crosses involving N. tabacum as one parentand aphid resistance donors viz., N. gossei, N.excelsior, N. x benthamiana-repanda, and N.umbratica as the other parents, were grown inprogeny rows along with 8 check varieties forpreliminary evaluation of leaf quality and yieldpotential. This includes 10 derivativesdeveloped from crosses, N. gossei x N. tabacumand N. umbratica x N. tabacum, screened andidentified as tolerant to leaf curl disease incollaboration with Entomologist. Fifteen lineswere identified as TMV resistant on artificialinoculation. In general, aphid infestation wasless during the season.

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25 ICAR - CTRI Annual Report 2015-16

for cured leaf, 15-31% for bright leaf and 15-31% for grade index.

Ten lines viz., TBST90 - 99 showed similaror better colour, body as well as size of curedleaf than Siri. The nicotine and reducing sugarlevels were within the admissible limits.

In the combined analysis of the last threeyears yield data , seven advanced crossderivatives viz., TBST Nos. 88, 91, 92, 93, 94,98 & 99) exhibited significantly superiorperformance over best check, Siri for all thefour yield traits while TBST 89 & 90 showedsignificant superiority for cured and bright leafyield and grade index. The superior linesexhibited 15 to 25% increase in green leaf yield,10 to 25% in cured leaf, 6 to 16% in bright leafand 7 to 17% for grade index, respectively overthe best check, Siri.

The entries showing significantimprovement for all the traits over checks duringall the 3 seasons were: TBST Nos. 92, 93, 94, 98& 99; four other lines viz., TBST Nos. 88, 90, 91& 96 showed significant improvement overchecks during 2 seasons.

Seasonal differences were significant amongyield traits. Season x entry interaction wasalso significant for all the four yield traits. Yieldperformance of the entries evaluated in theexperiment during individual seasons.

Based on overall performance the entriesviz., TBST Nos. 99, 88, 90, 91, 92, 93, 96 & 98were selected for further studies.

ii) Trial TBL-11 (3rd year)

Another replicated yield trial was conductedwith twelve morphologically stable, aphidresistant/ tolerant advanced cross derivatives(TBST 100 to TBST 111) along with two checks,Siri and VT 1158 in a RBD with 3 replications forevaluation of yield potential and leaf quality.All the breeding lines were light cast in nature.

Ten of the derivatives (TBST100-105, 107,108, 110 & 111) were identified as resistant toTMV also. Nine lines viz., TBST-100, TBST-101,TBST 102, TBST-104, TBST 105, TBST 107, TBST-109, TBST 110 and TBST-111 showed good colour,

Details of inter specific cross derivativesmaintained.

Sl. Initial crossNo.

1. (N. gossei x cv. CM-12)2. cv. HR 62-9 x N. gossei3. [(N. x gossei-excelsior) x CM-12]4. (N. x benthamiana-repanda) x cv. CM-125. Delcrestx[N. gosseixN. glutinosa)xCM-12]6. Delcrest x [N. gossei x N. glutinosa) x

[cv.HR 62-9 x N. gossei) x Bhavya]7. N. umbratica x N. tabacum

2. Preliminary evaluation for leaf yieldpotential:

Twenty two light cast derivatives with high curedleaf yield potential (CLY 2600 – 3450 kg/ha) ascompared to check variety, Siri (CLY- 1825 kg/ha) were identified. After evaluation of planttype, leaf number, plant height, floral, fertilitytraits along with cured leaf colour, size and body,and seed bearing nature, 20 promising uniformlines were retained for further evaluation inreplicated yield trials.

3. Evaluation of advanced lines in RYTs:

Six replicated yield trials were conductedduring the season with 72 advanced stabilizedinterspecific cross derivatives.

i) Trial TBL-10 (3rd year)

A replicated yield trial was conducted withtwelve morphologically stable, aphid resistant/tolerant advanced cross derivatives (TBST 88 toTBST 99) along with two checks, Siri and VT 1158in a RBD with 3 replications for evaluation ofyield potential and leaf quality. All the breedinglines were light cast in nature. Nine of thederivatives were reported as resistant to TMVunder artificial screening.

Differences among entries were significantfor all the four yield traits. Seven lines viz.,TBST 88, TBST 90, TBST 91, TBST 92, TBST 93,TBST 98 and TBST 99 showed significantimprovement over best check, Siri. The increasevaried from 17-34% for green leaf yield, 16-31%

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body as well as the size of cured leaf, ascompared to check varieties.

Differences among entries were significantfor all the four yield traits. Eight lines, viz.,TBST Nos., 100, 101, 105, 106, 107, 108, 109and 111 showed significant improvement overthe best check, Siri for all the four leaf yieldtraits.The increase was 23 to 34% for green leafyield, 22 to 34% for cured leaf yield, 25 to 41%for bright leaf yield and 25 to 39% for gradeindex, respectively, over Siri. The nicotine andreducing sugar levels were within the admissiblelimits, as observed from the results of previousstudies.

In the combined analysis, ten advancedcross derivatives viz., TBST-100, TBST-101, TBST-104, TBST 105, TBST 106, TBST 107, TBST 108,TBST-109, TBST 110 and TBST-111 exhibitedsignificantly superior performance over bestcheck, Siri for all the four yield traits during allthe 3 seasons. The superior lines exhibited 12to 33% increase in green leaf yield, 11 to 32% incured leaf, 14 to 19% in bright leaf and 15 to35% for grade index, respectively over the bestcheck, Siri.

Based on overall performance the entriesviz., TBST-100, TBST-101, TBST-104, TBST 105,TBST 106, TBST 107, TBST 108, TBST-109, TBST110 and TBST-111 were selected for furtherstudies.

iii) Trial TBL-12 (2nd year)

A replicated yield trial was conducted forthe second year with eight morphologicallystable, aphid resistant/ tolerant advanced crossderivatives (TBST 112 to TBST 119) along withtwo checks, Siri and VT 1158 in a RBD with 3replications for evaluation of yield potential andleaf quality. All the breeding lines were lightcast in nature. Five of the derivatives(TBST112, 113, 115, 116 & 117) are resistant toTMV also. All the entries along with checksshowed good colour, body as well as the size ofcured leaf.

Differences among entries were significantfor all the four yield traits. Only one line, TBST-115 showed significant improvement over thebest check, Siri for all the four leaf yield traits.

The increase was 16% for green leaf yield, 16%for cured leaf yield, 24% for bright leaf yieldand 21% for grade index, respectively. Nicotineand reducing sugars in cured leaf were indesirable limits in all the advanced interspecificcross derivatives during the season.

iv) Trial TBL-13 (2nd year)

Another replicated yield trial was conductedfor the second year with eight morphologicallystable, aphid resistant/ tolerant advanced crossderivatives (TBST 120 to TBST 127) along withtwo checks, Siri and VT 1158 in a RBD with 3replications for evaluation of yield potential andleaf quality.

Differences among entries were significantfor all the four yield traits. Five lines, viz.,TBST Nos., 121, 122, 125, 126 and 127 showedsignificant improvement over the best check,Siri for all the four leaf yield traits. The increasein these lines was 13 to 26% for green leaf yield,13 to 23% for cured leaf yield, 12 to 27% forbright leaf yield and 14 to 26% for grade index,respectively. Nicotine Content and reducingsugars in cured leaf were in desirable limits inall the advanced interspecific cross derivativesduring the season.

v) Trial TBL-14 (2nd year)

Another set of eight morphologically stable,aphid resistant/ tolerant advanced crossderivatives (TBST 128 to TBST 135) wereevaluated along with two checks, Siri and VT1158 in a RBD for yield potential and leaf quality.Four of the derivatives, TBST 129 – 132 wereidentified as resistant to TMV

Differences among entries were significantfor all the four yield traits. Fivelines, viz., TBSTNos., 128, 129, 130, 132 and 133 showedsignificant improvement over the best check,Siri for all the four leaf yield traits. The increasewas 20 to 24% for green leaf yield, 17 to 22% forcured leaf yield, 21 to 26% for bright leaf yieldand 21 to 25% for grade index, respectively.

vi) Trial TBL-15 (1st year)

A new replicated yield trial was conductedwith a set of twelve morphologically stable,

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27 ICAR - CTRI Annual Report 2015-16

aphid resistant/ tolerant advanced crossderivatives (TBST 136 to TBST 147) along withtwo checks, Siri and VT 1158 in RBD forevaluation of yield potential and leaf quality.

All the breeding lines were light cast innature. Nine of the lines viz., TBST 136, 137,139-141, and 144-147 were resistant to TMV alsounder artificial inoculation.

Differences among entries were significantfor all the four yield traits. Four lines, viz.,TBST Nos., 136, 140, 144 and 147 showedsignificant improvement over the best check,Siri for all the four leaf yield traits. The increaseover Siri was 13 to 35% for green leaf yield, 13to 37% for cured leaf yield, 15 to 33% for brightleaf yield and 11 to 33% for grade index,respectively.

Bulk and on-farm evaluation:

Advanced breeding line TBST-2 whichshowed superiority over check varieties atRajahmundry, Guntur and Kandukur inmultilocation trials was evaluated against Sirifor yield and quality in a bulk trial. The leafyield of TBST 2 (CLY 2634 kg/ha) was higher thanSiri (CLY 2113 kg/ha)

The line TBST-2 was also given for on-farmdemonstration in SBS (4 farmers), SLS (7farmers) and NBS (1 farmer) in collaborationwith Tobacco Board and Trade. The feedbackfrom the farmers was positive. The line wasreported to be slower maturing than Siri,thereby enabling leaf harvest intervals to suitbarn availability. Farmers also reported thatTBST-2 was also higher yielding than Siri inaddition to having resistance to TMV, toleranceto aphid and leaf blight (in nursery). The higher(13.3 – 16.8%) yield potential (3140 kg/ha in NBS,2440 kg in SBS and 1677 kg in SLS) and high BCratio (1.36 in NBS, 1.32 in SBS & 1.11 in SLS) inaddition to resistance traits and slow maturityand desirable leaf chemistry were preferred bythe farmers.

Location specific evaluation of crossderivatives

Promising derivatives having resistance totobacco aphid, caterpillar and those tolerant

to leaf curl, identified under the project andwere contributed to NLS (11) and KLS (66) forfurther evaluation.

Developing tobacco cultivars resistance tobiotic and abiotic stresses

Incorporation of disease resistance forTobacco Mosaic Virus (TMV) (CTRI,Rajahmundry)P.V. Venugopala Rao and S.K. Dam

Incorporation of Black Shank resistance inFCV varieties/ advanced breeding lines:

Black shank resistance incorporation in therecently released variety Siri and the advancedbreeding lines N-98 and Cy-142 are in progress.These lines were crossed with the resistantdonors Beinhart 1000-1 and 1129SR.

During 2014-15, 524 progenies wereevaluated under artificial inoculation with thepathogen. Out of 524 progenies, 233 progeniesrecorded resistance in all the plants.

Maintenance of the TMV resistant lines

TMV resistant lines viz., VT-1158, JMR,HMR, 1099/2/4, L-1358, L-1359, L-1366, L-1416,L-1417 and L-1419 were maintained underartificial inoculation. Twelve TMV resistant Natutobacco lines PVM 1 to 12 were also maintained.

I (B) Tailloiring tobacco plant type foroptimizing the seed yield andphytochemicals

Developing tobacco cultivars for high seedyield, oil content, high biomass and otherphyto-chemicals (CTRI, Rajahmundry)A.V.S.R.Swamy, T.G.K. Murthy, K. Siva Rajuand S. Kasturi Krishna

Two sets consisting of 28 F1 hybrids alongwith 8 parents in each are evaluated inreplicated yield trail. The first set is assessedfor seed yield and oil content while the secondset is evaluated for green leaf, total biomassand phyto chemicals viz., nicotine, solanesoland protein content. In first set (RYT-I) crossesTI-163 x GT-7 and GT-7 x GT-8 exhibited highernumber of primary branches (3.58 and 3.50) and

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the differences are significant. The crosses TI-1112 X GT-7 and GT-7 X A-119 (7.13 & 6.33)exhibited higher number of secondary branchesand the differences are significant. The crossesGT-7 X NP-19 (300 mg), A-145 X NP-19 (280 mg)and TI-163 X GT-7 (280 mg) recorded high weightof single capsule and the differences aresignificant. EC.554900 X A-145 (930 kgs),EC.554900 X A-119 (736 kgs) and parent A-145(770 kgs) exhibited higher total seed weight withsignificant differences.

(30.39) exhibited higher content and thedifferences among the entries are notsignificant. For total protein per ha GT-7 X VDH-3 (458.70), HDBRG X GT-8 (258.22), and parentAbirami (453.45) showed higher values.

Breeding for high seed and oil yield in tobaccoM.Kumaresan

From the forty F5 populations of fivepromising crosses grown under 75cm x 60cmspacing for high seed yield, 10 selections whichrecorded seed yield ranging from 1500 – 1950kg/ ha were retained for replicated yieldevaluation for seed and oil yield in the comingseason.

I. (C) Production and distribution of pure seedof ruling tobacco varieties

One of the main activities of the CentralTobacco Research Institute is the production anddistribution of pure seed and quality seedlingsof approved tobacco varieties to the farmers.The “Revolving Fund scheme” of CTRI has beenwell appreciated by the ICAR, New Delhi andthe tobacco farmers. About 6000 kg of truthfullylabelled seed of seven different varieties wassold to farmers through CTRI, Rajahmundry andits Research Stations and Tobacco Board.

(D) Germplasm resource management

Germplasm Acquisition, Maintenance,Evaluation and Utilization (CTRI,Rajahmundry)T.G.K. Murthy

A. Acquisition

Seventy five bidi and 5 exotic FCV lines wereadded to the germplasm bank.

B. Maintenance

(i) Cultivated germplasm: The total availablegenetic resources at CTRI are 3369. A total of2056 cultivated germplasm lines including FCV,non-FCV and elite lines were maintained.Theelite lines included released varieties; lines withhigh seed bearing, low nicotine, insect pestresistance, disease resistance, root knotresistance, CMS and high yield potential etc.

In the second set of crosses (RYT-II)evaluated ABHIRAMI X A-119 (2.49), A-119 X GT-8 (2.45) and Parents A-119 (3.27) and GT-6 (3.02)exhibited higher nicotine. For solanesol, leafcrosses Abirami X NP-19 (1.97) and HDBRG XVDH-3 (1.85) exhibited higher content and thedifferences among the entries are notsignificant. For protein percent A-119 X NP-19(26.09), GT-8 X NP-19 (24.96), and parentAbirami (31.71) showed higher values. Thecrosses NP-19 X VDH-3 (17076 kgs), GT-7 X GT-8(16299 kgs) and GT-7 X Abirami (16188 kgs)exhibited higher green leaf per ha and thedifferences were significant among each other.The crosses NP-19 X VDH-3 (24351 kgs) HDBRGX Abirami (24220 kgs) and HDBRG X VDH-3 (24099kgs) exhibited higher total biomass per ha., andthe differences were significant.

In the second set of crosses (RYT-II)evaluated GT-7 X HDBRG (43.59), Abirami X VDH-3 (45.23) and Parents A-119 (46.10) and GT-7(45.94) exhibited higher total nicotine per haand the differences are not significant. For totalsolanesol per ha crosses Abirami X NP-19 (33.98),HDBRG X Abirami (30.41) and GT-7 X HDBRG

A119 X Abirami

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29 ICAR - CTRI Annual Report 2015-16

After thorough examination for purity anduniformity, all the lines were selfed and seedcollected.

(ii) Wild Nicotiana species: A total of 210accessions of 56 wild Nicotiana species and twosubspecies were maintained in pots /experimental micro plots. Also, 9 exoticinterspecific hybrids, 5 hybrids developed atCTRI and 4 amphidiploids were maintained.Eleven non-flowering accessions were rescuedthrough in-vitro micro propagation.

C. Enhancement of variability: Interspecifichybrids, viz., N. sylvestris x N. tomentosiformisand N. sylvestris x N. otophora which areconsidered as the progenitor crosses ofcultivated N. tabacum were synthesised andtheir back cross progenies (BC2S3) were advancedfor infusing additional variation into thecultivated species and enhancing the scope forfurther genetic improvement of the crop. Anumber of selections with normal N. tabacumplant type were selected for further studies.

D. Conservation

All the germplasm accessions maintainedduring the past five years have been stored indeep freezers at -10ºC. Also a sample of eachline is stored under ambient conditions. So farabout 1400 germplasm accessions of FCV, Burley,Jati, JAC, EAC, Oriental, Bulgarian, sources ofroot knot nematode resistance, and releasedvarieties have been deposited at NBPGR for longterm seed storage. Also, three hundred Rusticagermplasm lines and 140 wild Nicotiana speciesaccessions have been processed to deposit atNBPGR.

E. Distribution

During the year a total of 120 accessions ofboth wild and cultivated Nicotiana species weresupplied to 15 different researchers/organizations.

F. Molecular Characterization

a. Barcode markers for Nicotiana species: Forthe purpose of Cataloguing and developmentof reference database for genus Nicotiana basedon DNA barcoding, leaf samples from 160

Nicotiana accessions raised in the season werecollected and the digital images of therespective accessions were also captured. TotalGenomic DNA was isolated from all theaccession.

Preliminary analysis confirmed polymorphicnature of the intergenic spacer trnH-psbA withrespect to the amplicon length in the testspecies accessions which was tested by SangerSequencing Method. The study is to continuewith more bar code primers.

b. DUS characterization: During the year threegenotypes viz., ruling variety Siri, promisingadvanced interspecific cross derivative TBST-2and unique germplasm line, 324C werecharacterized for 53 Distinctness, Uniformityand stability (DUS) characteristics.

G. Evaluation

Evaluation for seed yield:

In a preliminary row trial, 11 previouslyidentified germplasm lines and advanced crossderivatives were evaluated along with threecheck varieties viz., HDBRG, A-145 and GT-7,for seed yield potential. Advanced derivativesof crosses viz., GT7 x TI163, A145 x GT-7 andGCMS12-3 along with check GT-7 and A-145 werepromising.

Seed yield under different planting densities

In another preliminary trial, 7 previouslyidentified lines comprising germplasm lines andadvanced cross derivatives were evaluated alongwith check variety A-145 for seed yield underfour panting densities viz., 0.8 x 0.6 m, 0.8 x0.3 m, 0.8 x 0.2 m and 0.8 x 0.15 m.

Analysis indicated differential response ofthe genotypes to varying densities .Advancedderivatives of crosses viz., GT7 x TI163, A145 xGT-7 and GCMS12-3 along with check GT-7 andA-145 were promising.

Seed samples of 96 released tobaccovarieties besides seed samples of 126 wildNicotiana species accessions have beencollected, processed for estimation of oilcontent and fatty acid composition fordocumentation purpose.

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ICAR - CTRI Annual Report 2015-16 30

H. Copyright: A copyright (SW-8169/2014dt.12.11.2014) was granted by the CopyrightOffice of the Ministry of Human Resources, Govt.of India to the Nicotiana species informationsystem database.Obtaining of another copyrighton Natu germplasm is in progress.

Germplasm maintenanace of Nicotianatabacum/lines(CTRI RS, Hunsur)C. Nanda and S.S.Srinivas

Active stock of around 635 germplasmaccessions are maintained. Under the periodicalseed multiplication programme, 170 germplasmaccessions were regenerated . Male sterile linesof Kanchan and Rathna were maintained andincorporation of male sterility from variedsources into Kanchan, Rathna, Coker 371G, FCH201 and FCH 222 was carried out.

Evaluation and maintenance of Chewing,Cheroot and Cigar germplasm (CTRI-Vedasandur)Dr.M.Kumaresan and M.Mohan

Maintenance of germplasm

As a regular programme, 85 chewing and60 cigar and cheroot germplasm accessions wereraised, self pollinated and seed collected formaintenance.

Maintenance of male sterile lines

Cytoplasmic male sterile lines ofBhagyalakshmi, Abirami, Maragadam, PV-7, I-115, and VR-2 were crossed with their respectivefertile counterparts and seeds collected formaintenance of the male sterile lines.

Collection, evaluation and maintenance ofJati, Motihari, Cigar Wrapper & filler tobaccogermplasm (CTRI RS, Dinhata)S. Mandi

Maintenance of germplasm

Ten plants each of 70 lines of N. tabacum(Jati tobacco) and 185 lines of N. rustica(Motihari) tobacco were grown and 3 healthyplants in each line were selfed and selfed seedsof each line were collected separately for usein the ensuing season.

I. (E) Biotechnology for TobaccoImprovement

Molecular mapping of important tobacco(CTRI, Rajahmundry)K. Sarala, K. Prabhakara Rao, T.G.K. Murthy,K. Siva Raju and P.V. Venugopala Rao

Characterization of parents and mappingpopulations

Twenty two SSR markers were screened toidentify the polymorphism between the parentsof RIL population developed for the mapping ofTSNA, nicotine and solanesol traits. Eleven SSRmarkers viz., TM51534, TM10606, TM10891,TM50469, TM1111062, TM50868, PT61653,PT10375, PT50065, PT60723 and PT53345 werefound to be polymorphic.

Solanesol content was estimated in the aircured leaf samples collected from the solanesolmolecular mapping population and its parents.One of the parents HDBRG recorded highersolanesol (2.50%) and other parent By-53 lower(1.40%). Solanesol in the mapping populationfound to be in the range of 0.5-4.90. Thisindicates that transgressive segregation forsolanesol in the mapping population. Out of257entries analyzed in the mapping populationhighest population found to be in the range of2.0-2.5% and 2.5-3.0%.

Range of solanesol content in a solanesolmolecular mapping population and its parents(Air-cured samples) (2014-15)

S.No. Solanesol (%) Populationrange frequency

1 0-0.5 12 0.5-1.0 103 1.0-1.5 264 1.5-2.0 415 2.0-2.5 606 2.5-3.0 667 3.0-3.5 378 3.5-4.0 119 4.0-4.5 410 4.5-5.0 1Total 257

Nicotine content was estimated in the aircured leaf samples collected from the nicotine

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31 ICAR - CTRI Annual Report 2015-16

molecular mapping population and its parents.Among the parents, Candel recorded highernicotine (2.71%) and Nisnicotinony-121 lower(0.90%). Nicotine in the mapping populationfound to be in the range of 0.34-4.94. Thisindicates that wide variation is created in themapping population. Out of 248 entries analyzedin the mapping population highest populationfound to be in the range of 0.5-1.0% and 1.0-1.5%.

Development of mapping populations

For the development of mappingpopulations i.e. Recombinant Inbred Lines(RILs), 9 F1s and11 mapping populations viz.,BY 64 x Banket A1, VA 510 x Banket A1, HDBRGx BY 53, HDBRG x GT-7, TI 163 x A-145, Candelx Nisnicotinony 121, Kumkumathri xNisnicotinony 121, Nisnicotinony 121 xKumkumathri, A 145 x GT 7, GT 7 xA 145 andA 145 x Jayalakshmi-WS (a total of around 2200plants) were raised and selfed seed collected.Thirteen dihaploid lines were developed fromfive crosses were maintained.

and GT5 were infected with Pythiumaphanidermatum and samples were collectedbetween 12 to 36 hrs after infection at differenttime intervals. Total RNA was isolated from theinfected and control seedlings and cDNA wassynthesized using reverse transciptase PCR. Theintegrity and quality of the cDNA was analysedusing housekeeping genes, Ribulosebisphosphatecarboxylase small subunit (Rbcs) and Elongationfactor 1α (EF-1α).

In the expression analysis of defense relatedgenes mitogen-activated protein kinase 2(NtMEK2), other effector protein genespathogenesis-related protein (PR1a),Phenylalanine Ammonia-Lyase 1 (PAL1) and Beta-1, 3-glucanase gene (Glunse) has shownprominent increase in their transcript levels in

Polymorphism among the parents of RILpopulations of nicotine and solanesol with SSR

primer, PT50469

Transcript profiling and identification ofcandidate genes resistant to Damping-off intobacco (CTRI, Rajahmundry)K. Prabhakara Rao, K. Sarala, T.G.K. Murthy,S. K. Dam and K. Sivaraju

The ITS (Internally Transcribed Sequence)regions of the 21 fungal isolates collected fromdifferent regions of damping off effectedtobacco nurseries were sequenced andidentified as Pythium isolates by BLAST ( BasicLocal Search Alignment Tool) analysis. Four weekold seedlings of tobacco genotypes Coker1, GT9

Expression of defense related genes in tobaccogenotypes under Pythium infection

tolerant genotypes. It implies the associationof these genes with the disease tolerancemechanism.

Molecular characterization and cataloguing ofgenus Nicotiana using DNA barcoding (CTRI,Rajahmundry)K. Prabhakara Rao, K. Sarala and T.G.K. Murthy

Leaf samples from 160 Nicotiana accessionsraised in the season were collected and thedigital images of the respective accessions werealso captured. Total Genomic DNA was isolatedfrom all the accessions and screening wascarried out in selective Nicotiana accessionswith trnH–psbA (intergenic spacer region) whichis a rapid evolving loci in a loci specificPolymerase Chain Reaction (PCR) and theamplicons were analyzed by Poly Acrylamide GelElectrophoresis (PAGE). The results revealedthat the trnH –psbA loci is continued to be

Tobacco Cultivar Development

ICAR - CTRI Annual Report 2015-16 32

polymorphic with respect to amplicon length inthe selected Nicotiana accessions. Theseamplicons were sequenced by Sanger sequencingwhich further confirmed the length andsequence variation of the loci in the selectiveaccessions.

Molecular finger printing of pipeline varieties/germplasmaccessions

In order to develop varietal fingerprints, fivepipeline varieties viz., Tobios-2, JS-117, NLST-4, TBST-2, and YB-4, two popular varietiesKanchan and BanketA1, and a germplasmaccession, 324C were screened with fifteen SSRmarkers. Primers, NSTM-12, PT-51706, PT-53418,TBM-36 and TM-10163 were found polymorphic.

VARIETIES RELEASED BY ICAR-CTRI (2015-16)

● LT KANCHAN : A low Tar flue-cured Virginiatobacco variety with high yield potentialof 2,500 kg/ha is suitable for commercialcultivation in the Northern light soil regioncovering West Godavari district of Andhra

Pradesh. Low tar content (less by 10%) incured leaf biomass is the special traitassociated with this variety.

● CH-1: A CMS based flue-cured Virginiatobacco hybrid is released for commercialcultivation in the Northern light soil regionof AP. This hybrid is known for its high yieldpotential (2900 kg/ha) as well as itssuperior leaf quality in terms of smokeflavor resulting from relatively higherconcentration of neutral volatilecompounds.

● N-98: A high yielding (2,200 kg/ha) FCVtobacco variety is suitable for commercialcultivation in Southern Light Soils regionof Andhra Pradesh. It is the most preferedtobacco variety in the recommended area.

● Nandyala Pogaku-1: It is the first biditobacco variety released for commercialcultivation in Nandyal region of AndhraPradesh and has yield potential of around2200 kg/ha. It performs well in the droughtconditions.

Tobacco Cultivar Development

33 ICAR - CTRI Annual Report 2015-16

II. Development of Agro-technology forSustainable Tobacco Production andStrengthening TOT

II(A): Optimisation of Water& Nutrient Usefor Productivity Enhancement of DiffereentTobacco Ttyes

Effect of drip irrigation and tray seedlings onthe productivity of NLS tobacco (CTRI,Rajahmundry)S. V. Krishna Reddy, C. Chandrasekhara Raoand S. Kasturi Krishna

The field experiment was conducted duringrabi, at ICAR-CTRI RS, Jeelugumilli, with FCVtobacco (Nicotiana tabacum L.) cv.Kanchan inirrigated Alfisols (northern light soils) of AndhraPradesh. The experiment consisted of 9treatments replicated five times in RBD withdifferent combinations of drip irrigation,Furrow irrigation, tray seedlings, normalseedlings, drip fertigation and soil applicationof fertilizers at 3rd, 25-30, 40-45 days afterplanting or at 10, 25-30, 40-45 days afterplanting. Results revealed that significantdifferences between the treatments with regardto the yield characters studied. Drip irrigation,tray seedlings, drip fertigation at 3, 20-25 and40-45 DAP followed by drip irrigation, trayseedlings, drip fertigation at 10, 25-30 and 40-45 DAP recorded higher green leaf yield, curedleaf yield, grade index, green leaf/cured leafyield and grade index/ cured leaf (%) whencompared to other treatments. The treatmentconsisting of drip irrigation, tray seedlings, dripfertigation at 3, 20-25 and 40-45 DAP increasedgreen leaf yield by 3,556 kg (23.87%), cured leafyield by 415 kg (16.67%) and grade index by 450kg (23.44%) when compared with furrowirrigation, normal seedlings, soil application offertilizers at 10, 25-30 and 40-45 DAP. Amongall the treatments, furrow irrigation, normalseedlings and soil application of fertilizersrecorded the lower yields. The tobacco yieldsare significantly higher during 2014-15 cropseason due to favourable weather conditions.

Quality: The differences among treatmentswere also marked in quality parameters. Ingeneral, nicotine levels increased from P to T

position and reducing sugars increased from Pto L position and decreased from L to T position.Among the treatments drip irrigation, trayseedlings, drip fertigation at 3, 20-25 and 40-45 DAP and drip irrigation, tray seedlings, dripfertigation at 10, 25-30 and 40-45 DAP recordedhigher levels of nicotine and lower levels ofreducing sugars as compared to furrowirrigation, normal seedlings and soil applicationof fertilizers (T9) i.e. The treatment T9 recordedthe lower levels of nicotine and higher levelsreducing sugars. The chloride levels are wellwithin the acceptable limits of good quality (<1.5%).

Table II-1.Effect of drip irrigation and trayseedlings on the Productivity (kg/ha) in NLStobacco

Treatment Cured leaf Grade index

T1 2904 2370T2 2832 2332T3 2489 1920CD @5% 104 88

T1 : Drip irrigation, tray seedlings, dripfertigation at 3, 20-25 and 40-45 DAP

T2 : Drip irrigation, tray seedlings, dripfertigation at 10, 20-25 and 40-45 DAP

T3 : Furrow irrigation, normal seedlings, soilapplication of fertilizers at 10, 25-30 and40-45 DAP

Alternative and Cheaper Source of Nitrogento FCV tobacco in Northern Light Soils ofAndhra Pradesh (CTRI, Rajahmundry)C. Chandrasekhararao, D. Damodar Reddy,S.V. Krishna Reddy and S. Kasturi Krishna

Ten treatment combinations of urea, AS,KNO3 and CN in three splits were compared withDAP + CAN + SOP (Recommended Dose), and 20-20-0, SSP, Urea, Ammonium Sulphate and SOPcombinations (Interim Fertiliser schedulerecommended). Application of urea/ AS as abasal dose followed by urea or AS or Urea+ ASor Urea + AS+ KNO3 or Urea + AS + CN did not

ICAR - CTRI Annual Report 2015-16 34

AS+ Urea + AS 1875 1458AS + Urea + AS + AS 2005 1554AS + AS + AS 1772 1382AS + Urea + AS + KNO3 + AS 1981 1580AS + Urea + AS + CN + AS 1871 1389DAP + CAN + CAN 2036 155720-20-0 + Urea + AS 2076 1625CD at 5% NS NS

Feasibility of Producing Organic Tobacco in KLS(CTRI RS, Hunsur)M. Mahadevaswamy and S. Ramakrishnan

The studies on the feasibility of producingorganic tobacco using various organics (FYM @12 t/ha, use of bio-fertilizers @ 10 kg/ha, greenmanuring in rabi season with sunnhemp, use ofneem based organics and bio-pesticides etc.,)was conducted for the fourth consecutive cropseason during 2014-15 (for the second cycle) inHunsur farm. The results of the 4th year indicatedthat the reduction in the productivity of theorganic tobacco was 28.5% compared to thereduction of cured leaf to an extent of 50%,42.7% and 33.0% observed in the first, secondand third crop season respectively. The INMtreatments involving organic and inorganic at75:25 and 50: 50 ratios resulted in reduction inthe yield comparatively (16.5 and 8.5 %respectively). Similarly the total top grade leafproduction reduced by 22.5% in the organictreatment while the same in the INM treatments(75:25 and 50: 50) resulted in 11.7 and 6.4%reduction respectively. However, the brightgrade production was higher by 6-7% in theorganic treatment.

With respect to the cured leaf qualitycharacteristics, the nicotine in the X positionwas lower (0.68%) in the organic treatment whencompared to the inorganic treatment (1.23%).The L position leaf also showed similar trendswith recommended NPK treatment recording thehigher nicotine value of 2.17% compared to1.47% in the organic treatment. With regard tothe disease incidence, there was reduction inthe root knot incidence by about 40.7% andreduction in the wilt incidence by 38.5% in thefully organic treatment.

show significant differences in green leaf yieldor cured leaf yield or grade index.

Nicotine content in lamina was low andreducing sugars were high. Nicotine in laminaincreased and reducing sugars decreased withincrease in plant position. Different treatmentcombinations did not show any significantdifferences in nicotine, reducing sugars andnitrate nitrogen. Nitrogen content in laminadid not show any significant difference due tonitrogen sources. In general, the nitrogenconcentration in different treatments was lowespecially in L and T positions. Theconcentration of P and K was also notsignificantly differed among the treatments. Ingeneral potassium concentration was low.

Effect of Fertiliser Source of Nutrients on Yieldand Quality of Burley Tobacco Grown in Uplands (CTRI, Rajahmundry)S. Kasturi Krishna, S.V. Krishna Reddy and K.Siva Raju

Sources of fertilizer affected the yield andquality of burley tobacco significantly.Application of CAN/Ammonium sulphate in 1st

dose along with DAP and CAN in 2nd dose gavehigher green and cured leaf yields. A/S + SSP in1st dose with A/S in second dose and 28:28 :0in 1st dose with A/S in second dose are the nextbest treatments. In case of non availability ofCAN, either A/S or 28:28:0 may be used in burleytobacco production.

Nicotine and reducing sugars are within inthe desirable limits. Significant differences wereobserved for the above parameters. However,higher values of Nicotine were observed whenA/S was applied in both the doses. Differenceswere not observed in the treatments forreducing sugars.

Table II-2: Effect of different nitrogen sourceson yield (kg/ha) Parameters

Treatments Cured GradeLeaf indexyield

Urea+ Urea + AS 2111 1689Urea + Urea + AS + AS 1932 1507Urea + AS + AS 2002 1550Urea + Urea + AS + KNO3 + AS 2177 1745Urea + Urea + AS + CN + AS 2119 1665

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

35 ICAR - CTRI Annual Report 2015-16

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

Table II-3:Yield parameters (Kg/ha) asinfluenced by organic & INM treatments

Treatments Cured Topleaf gradeyield equivalent

Fully organic 912 73075% organic + 25% inorganic 1071 83150% organic + 50% inorganic 1164 881Recd. NPK 1272 942

Recommended NPK 60:40:120 kg/ha

Potassium Nutrition Management Strategiesfor Productivity and Quality Enhancement ofFCV Tobacco Grown Under Rain FedEnvironment in KLS (CTRI RS, Hunsur)M. Mahadevaswamy, and C.Chandrashekar Rao

Under this project, the experiment withvaried K levels and split/time of applicationswas conducted both in nursery and main field.

Nursery trial: The nursery experiment showedpositive response in terms of seedling vigor,seedling root and shoot length and seedling drymatter production up to 100 kg K2O/ha.Application of 100 kg K2O/ha in 4 splits (atsowing, 25, 35 and 45 DAP) recorded significantlymaximum seedling growth parameters, drymatter production and higher number oftransplantable seedlings per unit area comparedto 25 kg or 50 kg/ha. The incidence of RKI wasalso considerably reduced under higher level ofK applications with more split doses.

Main felid trial: In the second phase of theexperiment, the best performed nurserytreatment was selected for planting in the mainfield and evaluated with various combinationsof K2O levels with varied split applicationmethods to meet the K requirement of thetobacco crop for optimizing the productivity andenhancing quality.

The effect of K levels and their splitapplications on the plant growth parametersreveal that all the treatments with more thanone split application of K showed increased leafarea of 9th ,12th and 15th leaf at 60 days comparedto only single application. Eventhough theincrease in the leaf area was not statistically

significant, the split applications of K in 3-4 splitsincreased the leaf area by 4.0-11.7 % in the 9th

leaf, 5.0 – 11.1% in the 12th leaf and by 2.0-14.5% in the 15th leaf. There were no significantdifferences in the growth parameters between90 kg and 120 kg K2O/ha.

All the treatments with various splitapplications of K were significantly superiorcompared to no K application with respect tocured leaf yield. The increase in cured leaf wasmaximum at 120 kg K2O/ha applied in 4 splitswith an increase of 23% compared to no Kapplication and 10.3% compared to K applicationonly as basal dose. Treatment with 120 kg K2O/ha applied in 4 splits at (10, 25, 40 & 55 DAT)recorded the maximum TGE and wassignificantly superior to the same dose at onesplit or 2 splits. The increase in the productivityof TGE due to 3-4 split application of potassiumwas in the range of 15.6 to 20.7% indicating theusefulness of potash applications in more than2 splits.

Table II-4: Effect of potassium levels and splitapplications on yield parameters

Treatments CLY TGEKg/ha Kg/ha

60:40:120kg NPK/ha (10) 958 67660:40:120kg NPK/ha (10 & 25) 1013 71360:40:120kg NPK/ha((10, 25 & 40) 1046 78260:40:90 kg NPK/ha(10, 25 & 40) 1038 80260:40:120 kg/ha (10, 25,40 & 55) 1057 81660:40:90 kg/ha (10, 25, 40& 55) 1043 79260:40:0 (No K) control 857 545C.D. at 5% 116.6 95.9

Permanent Manurial Trial on Motihari Tobacco(CTRI RS, DINHATA)S. Mandi & D.Damodar Reddy

Results of the permanent manurial trialshowed that the application of 112 kg N + 112Kg P2O5 + 112 Kg k2O ha-1recorded significantlyhigher green leaf (15875.32kg ha-1) and curedleaf (2331.7 kg ha-1) yields of Motihari tobaccoas compared to control with only FYM @10q ha-

ICAR - CTRI Annual Report 2015-16 36

1. First grade leaf yield was significantly higherin treatment 112 kg N + 112 kg P2O5 ha-1(954.7kgha-1) than control FYM @10 q ha-1. Applicationof phosphorus and potassium alone or incombination with each other and only 25 or 50t F Y M applied plots gave lower green, curedand first grade leaf yield and quality ascompared to the application of nitrogen aloneor in combination of phosphorus and potassium.

Application of 112 kg N ha-1 + 112 kg P2O5ha-1 + 112 kg K2O ha-1 recorded highest grossreturns (¹ 1,04,940 ha-1) followed by 112 kg Nha-1 +112 K2O ha-1 (¹ 1,01,835 ha-1) and 112 kg Nha-1+ 112 kg P2O5 ha-1(¹ 1.00,485 ha-1). However,net returns were higher in treatment 112 kg Nha-1 +112 K2O ha-1 (¹ 2,24,24 ha-1) followed bytreatments 112 kg N ha-1 + 112 kg P2O5 ha-1 +112 kg K2O ha-1(¹ 19999 ha-1) and 112 kg N ha-1+112 kg P2O5 ha-1(¹ 18643 ha-1).

Optimisation Dose of N, P, K with Spacing inPipe Line Variety of Jati Tobacco in NorthBengal (CTRI RS, DINHATA)S. Mandi and S.Kasturi Krishna

The experiment comprised of three spacings(75X75cm, 75X90cm and 90X90cm) and threeNitrogen levels (100 N ha-1, 125 N ha-1 and 150kg N ha-1) with three replications in FRBD.Spacing 90 X 75 cm was recorded highest green(7469.09 Kg ha-1) and cured leaf yield (1298.45Kg ha-1) and first grade leaf yield (929.68 Kg ha-

1) followed by treatment 75 X 75 cm. Howeverhigher quality out-turn was recorded in 75 X 75cm (73.81 %) than the rest of two treatments.Nitrogen dose 150 Kg ha-1 was recorded highestgreen leaf yield (7218.49 Kg ha-1), cured leafyield (1249.47 Kg ha-1) and first grade leaf yield(990.13 Kg ha-1).

Treatment 90 X 75 cm spacing incombination with application of 100 Kg ha-1

nitrogen recorded maximum gross returns (¹ /ha 79,500.00), net return (¹ /ha 5, 689.00) andB: C ratio (1:1.078) followed by spacing 90 X 75cm combination with application of 150 Kg ha-1

nitrogen was recorded Rs/ha 75,840, Rs/ha4,593 and Rs/ha 1:1.062 gross returns, netreturns and B: C ratio respectively. Treatment90 X 90 cm and 75 X 75 cm spacing withcombination 100,125 and 150 Kg ha-1 nitrogenrecorded low returns due to low market pricethis year.

Inference: Spacing of 90cm X 75 cm andNitrogen dose 150 Kg ha-1 recorded highestgreen, cured and first grade leaves, treatmentcombination 90 X 75cm with 100 Kg ha-1 wasmore economical.

Table II-5: Yield of Jati tobacco (kg/ha) asinfluenced by different spacing and Nitrogen

Treatments Cured First gradeleaf yield leaf yield

Spacings Main lots75 X 75 cm 1230 90890 X 75 cm 1298 92990 X 90 cm 1085 783CD at 5% 100 85

N Dose Sub plots100 N Kg ha-1 1192 823125 N Kg ha-1 1172 808150 N Kg ha-1 1249 990CD at 5% 89 74

II B. Evolving Site-specific CulturalManagement Practices in Different Agro-Ecological Sub Regions

Chemical Management of Orbanche in FCVtobacco (CTRI, Rajahmundry)S. Kasturi Krishna, S.V. Krishna Reddy andVSGR. Naidu

Yield: Field experiment was conducted during2014-15 season at CTRI farm Katheru. Resultsrevealed that significant differences wereobserved with regard to leaf yield of tobaccodueto chemical method of Orobanche control.Significantly higher green and cured leaf yieldwas recorded by PPI of Alachlor, followed byhand removal of orobanche, neem cake, A/Sspray, neem oil spray, soybean oil spray, PPI ofpendimethalin and lower yields in postemergence application of glyphosate &imazethapyr and in control when compared tohand removal of orobanche. In general, thegrade out-turn is very low due to pot water givenfor orobanche emergence in tobacco. Postemergence application of glyphosate &imazethapyr reduced the quality drastically.

Weight of Orobanche: In general the Orobancheemergence was lower in the experimental plot.Orobanche emergence was not observed in theplots where glyphosate and imazethapyr wereapplied as post emergence spray at 50 and 70days after planting. Application of chemicals viz.

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

37 ICAR - CTRI Annual Report 2015-16

Studies on False Maturity and its MitigationStrategies in FCV Tobacco Growing Zones ofAndhra Pradesh (CTRI, Rajahmundry)S. V. Krishna Reddy, M. Anuradha, S. KasturiKrishna and P. Venkateswarlu

Among the INM treatments, organic manure(FYM) + balanced NPK plot performed betterand produced higher green leaf yield, cured leafyield and grade index and has balancedchemistry and higher chlorophyll contentfollowed by FYM + Excess N+ recommended PKplot. These two treatments did not express anyfalse ripening symptoms. Only N without FYMand PK plot recorded lower yields and showedfalse ripening. The FYM+ balanced NPKtreatment recorded increased cured leaf yieldby 180 kg/ha (7.17%) and grade index by 117kg/ha (10.66%) as compared to the treatment,only N without FYM and PK. Among the varietiesthe cv.VT-1158 performed better followed byTBST-2 without false maturity. Though the cv.Siri recorded higher yields compared to TBST-2,false maturity was observed in cv. Siri. Higherchlorophyll content was recorded in VT-1158.

Among the inter-culture treatments, regularinter-culture with complete weeding andOrobanche (broomrape) removal recordedhigher GLY, CLY, GI, GI/CL (%), balancedchemistry and higher chlorophyll content in leaflamina. In reduced inter-cultivation and weedcontrol through chemicals with no inter-cultivation, the cured leaf yields are slightlyless than regular inter-cultivation treatment butgrade index is reduced to a considerable extent.These two treatments showed lower chlorophyllcontent and false ripening. The regular inter-culture with complete weeding and orobanche(broomrape) removal treatment recordedincreased cured leaf yield by 64 kg/ha (2.46%)and grade index by 112 kg/ha (9.47%) ascompared to the treatment, only N without FYMand PK. Among the varieties the cv.VT-1158performed better followed by TBST-2 withoutfalse maturity. Though the cv. Siri recordedhigher yields and nicotine content compared toTBST-2, false maturity was observed in cv. Siri.Higher chlorophyll content was recorded inTBST-2.

Among the INM treatments, FYM + balancedNPK (reco.) and FYM + (excess N) rec.PK plotsperformed better and recorded higher GLY, CLY,

neemcake, A/S spray, neem oil, soybean oil, preemergence application of Alachlor andPendimethalin reduced the orobanche weightwhen compared to control.

Leaf Quality: Leaf quality in terms of nicotineand reducing sugars are affected by chemicalcontrol of orobanche and are within thedesirable limits. Significantly lower reducingsugars were observed in PPI of alachlor and inthe control i.e. without removal of orobancheand in PEA of Glyphosate plots. Higher sugarswere observed in neem cake applied plots. Nosignificant differences were found betweentreatments for nicotine content.

Orobanche infestation was reduced andyield improvement was observed whenglyphosate was applied @ 50 g ai/ha whencompared to 200 g ai/ha application in theprevious season. No phyto-toxicity wasobserved. PPI of pendimethalin@ 325 g ai andalachlor 1000 g ai/ ha recorded on par yieldswith that of hand removal of orobanche. Postemergence application of imazethapyr @ 50g ai/ha caused deformation in tobacco leaf shapeand suckers were emerged from the stems,though the control of orobanche was reportedin many cases.

Table II-6: Yield of FCV tobacco (kg/ha) andOrobanche weight as affected by chemicalmethod of Orobanche control

Treatment Cured Orobancheleaf Freshyield wt g/

100plants

PPI of Alachlor 2157 213PPI of Pendimethalin 2069 278Neemcake application@10 g/plant 2097 354PEA of Glyphosate at 50DAP 1663 -PEA of Imazethapyr at 50DAP 1652 -PEA of Neem oil toOrobanche 2082 378Hand removal of Orobanche 2114 562Control 1774 636CD at 5% 271 155

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

ICAR - CTRI Annual Report 2015-16 38

GI, GI/CL(%) and also recorded higherchlorophyll and nicotine content. These plotsshowed healthy growth all through the cropseason and false maturity was not recoded.Balanced NPK and (excess N) + reco. PK plotsrecorded lower yields and lower chlorophyllcontent and reduced nicotine content. Falsematurity was recorded in these two plots.

Among topping/sucker control plots,decanol (2%) + pendimethalin (0.3%)performed better and showed healthy cropgrowth throughout the season followed bydecanol (4%) and pendimethalin (0.6%) as is alsoindicated by higher chlorophyll and nicotinecontents. Excess pendimethalin (1%), no toppingand manual de-suckering produced lower yieldsand showed false maturity. No topping recordedlower yields compared to all other treatments.

Among irrigation water management plotsexcess irrigation during grand growth period,irrigation as per schedule showed healthy growthwithout false maturity and recorded higher GLY,CLY, GI, GI/CL (%) and chlorophyll content ascompared to deficit irrigation during grandgrowth period. The deficit irrigation duringgrand growth period treatment showed falsematurity and slightly higher nicotine content.This might be due to lower yield andconcentration effect.

Among weed control / interculturaltreatments, regular inter-culture plotperformed better and recorded higher GLY, CLY,GI and chlorophyll content and maintainedhealthy crop growth all through the crop season.The plots of reduced inter culture with postemergence spray of quizalofop ethyl and PPI ofpendimethalin recorded lower leaf yieldsand also showed false maturity.

Studies on Climate Risk Management in FCVTobacco Based Cropping Systems in STZ ofKarnataka (CTRI RS, HUNSUR)M. Mahadevaswamy, and C. ChandrashekarRao

During the period under report, thecorrelation of the 30 years data (1984-2014)between the climatic factors like temperature,relative humidity, sunshine hours Vs productivityand quality of FCV tobacco in KLS were studied.

The effect of pre-monsoon rainfall as well asthe rainfall during the crop season on FCVtobacco productivity pattern and quality oftobacco also was studied. The productivity wasfound to increase with increase in the rainfalland the number of rainy days during the cropgrowing season. There was no significantcorrelation between the various climatic factorslike temperature, relative humidity, sunshinehours and the productivity or quality of FCVtobacco. Increase in temperatures during July/August months slightly increased the nicotine.Sunshine hours even-though did not showsignificant correlation, sun shine hours duringJuly/August months showed positive trend withthe productivity. There was a decreasing trendin the nicotine content with the increase in therainfall during crop growing season. The chloridecontent had a negative correlation with rainfallamount during the growing season.

Identification of Critical Stages of Weather forFCV Tobacco Productivity and Quality:

Based on the meteorological standardweeks, weekly rainfall (RF), weekly potentialevapotranspiration (PET) and ratio of RF/PETfor each of the standard weeks, data wassubjected for analyzing the length of growingperiod, and the probable dry spells and wetspells that could occur in the tobacco growingseason for a period of 20 years (1995-2014).

During the growing season the standard,23rd week as well as 26th week (June 2nd weekand 4th week) which are called dry spells (wherethe RF/PET is less than 0.50) seems to be criticalduring which the PET exceeds the rainfall by

Fig. II-1: Correlation of the rainfall data withthe cured leaf productivity

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

39 ICAR - CTRI Annual Report 2015-16

more than twice during which judicious droughtmanagement practices need to be adopted asthis stage coincides with the active vegetativegrowth. Similarly during the Std weeks 32nd -33rd (Aug 2nd and 3rd week), the crop is also likelyto suffer from slight moisture deficiency duringwhich moisture conservation techniques areneeded for optimum productivity. Howeverstandard weeks (34-44th week) are called as wetweeks or wet spells (Aug 3rd onwards to Octoberlast week) where amount of rainfall is more thanthe evapo-transpiration( RF/PET ratio is morethan 1.00) which requires separate excess watermanagement practices for productive tobaccofarming. The study indicates that the climaticrisks are more during the later harvesting stagesof the crop due to wet spells which is affectingthe quality of the crop apart from decreasingthe productivity in KLS Zone.

Evaluation of Set Row Planting in BurleyTobacco for Efficient Resource Conservationand Utilisation (CTRI, Rajahmundry)C. Chandra Sekhara Rao, D. Damodar Reddyand S. Kasturi Krishna

Progressive increase in fertilizer doses inSRP increased green leaf yield and cured leafyield. SRP 100% RDF showed significantly higherGLY over other treatments. SRP with 75% RDF,50% RDF and CP with 100% RDF being at parshowed significantly higher green leaf yieldover SRP with 25% RDF and SRP with nofertilisers. SRP with 100% RDF showedsignificantly higher cured leaf yield over othertreatments. SRP 75%, RDF is at a par with CPwith 100% RDF showed significantly higher yieldsover SRP with 25% RDF and SRP with nofertilizers.

SRP with different doses of fertilisers andCP with RDF being at par showed significantlyhigher nicotine compared to SRP with nofertilizers. Increase in dose of fertilizers in SRPshowed increase in leaf nicotine. Reducingsugars were significantly higher in SRP with nofertilisers compared to SRP with fertilizer dosesand conventional planting with RDF

Nutrient uptake showed progressiveincrease with fertilizer doses in SRP. SRP with100% RDF showed significantly higher N uptakeover other treatments. SRP with 75% RDF and

CP with 100% RDF being at par showedsignificantly higher ‘N’ uptake over SRP with 0and 25 % SRP. SRP with 100% RDF showedsignificantly higher ‘P’ uptake and was at parwith 75% RDF. SRP with 75% RDF at par with CP100% RDF. SRP with 100% RDF showedsignificantly higher potassium uptake comparedto other treatments. SRP with 75% RDF being atpar with 50% RDF and CP with 100% RDF showedsignificantly higher K uptake over 25% RDF andSRP with no fertilizers

Soil properties: 0-5 cm: Increase in fertilizerdoses in SRP increased the soil availablenitrogen, phosphorus and potassium. Continuousapplication of fertilizers without disturbing thesoil increased the soil available P significantlyin SRP 75% and 100% RDF compared toconventional planting. Available potassium inSRP with 100% RDF was significantly higher thanSRP with 25% RDF and no fertilizers. Availablenitrogen also increased with increase infertilizers in SRP. SRP with 100% RDF showedsignificantly higher N compared to othertreatments.

5-15 cm: Available P in SRP 100% RDF showedsignificantly higher P compared to CP with 100%RDF and SRP 0, 25, 50 and 75 % RDF. Increase infertilizer doses increased the phosphorus andpotassium build up in set row planting.

15-30 cm: SRP treatments were not significantlydiffered with respect to pH and chlorides butshowed significantly less pH and higher chloridescompared to CP with 100% RDF. Increase infertilizer doses in SRP showed increase inavailable P. SRP with 100% RDF showedsignificantly higher P compared to CP with 100%RDF. Set row planting with 100% RDF showedincrease in available K over conventionalplanting.

Agronomic Evaluation of Advanced BreedingLine HV.2009-3 (CTRI RS VEDASANDUR)M. Kumeresan

The advanced breeding line Hv.2009-3significantly increased the FGLY and TCLY overthe check, Abirami. The FGLY and TCLY increasedwas 10.6 and 13% respectively with HV.2009-3over Abirami.HV.2009-3, HV.2009-5 recorded acomparable FGLY and TCLY .The spacing 90x75cm significantly increased the FGLY and TCLY

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

ICAR - CTRI Annual Report 2015-16 40

.The FGLY and TCLY increased with 90cm x75cmspacing was 7.8 and 8.66% respectively. Nitrogenat different levels did not influence the FGLYor TCLY. Higher gross return, net return and B.Cratio were recorded at HV.2009-3 at 90cmx75cmand Nitrogen at 75 kg/ha.

II C. Analysis of Socio-Economics forStratification and to FormulateAppropriate Strategies

Impact Analysis of CTRI Technologies (CTRI,Rajahmundry)Y. Subbaiah and K. Sarala

The technologies which have completedthree years period from year of release wereselected to study the impact assessment. Thefollowing technologies were identifiedpertaining to KLS and NBS areas.

KLS: 1. Variety FCH-222 2. Application ofPropiconazole @ 0.05 to 0.10% againstRhizoctonia solani 3. Bio-intensive managementof soil borne diseases and root knot nematodesby Trichoderma viridi and Pseudomonasflorescence in nurseries and main field.

NBS: 1.Variety Siri 2. Hand removal ofOrabanche shoots before flowering, 2-3 yearsof crop rotation and insitu green manuring 3.NPK fertilization 4. Recommended interculturesand 5. Insect control measures.

During 2014-15 season, adoption ofrecommended measures for the control ofsoreshine disease and bio-intensive managementof soil borne diseases in disease prone nurseriesimproved the income of nursery growers byRs.26,400/- per ha. Further adoption of cv.FCH-22 and bio-intensive management of soil bornediseases in F.wilt endemic main fields improvedthe farmers income by Rs.31,520/-, per ha inKLS area. With regard to NBS area, the resultsrevealed that the adoption of cv: Siri improvedthe farmers’ income by Rs.16,560/- per ha.Adoption of technology module consisting of NPKfertilization, timely inter-cultures, managementof broomrape, insect control measures improvedthe farmers’ income by Rs.32,018/- per ha.

The average value of assets like barn, tractor,bulking shed, implements etc., in different FCV

tobacco growing areas of Andhra Pradesh whichwas found to be Rs. 11,28,720/- in NLS, Rs.7,61,975/- in BS and Rs.3,95,592/- in SLS.

Nutritional Security in Tribal Areas of EastGodavari District through Community BasedApproaches (DBT funded Project: CTRI,Rajahmundry)K SUMAN KALYANI & TGK MURTHY

The major objective of the project is toenhance the livelihood security of tribal farmfamilies by introducing nutrition related locationspecific technologies and income generation andentrepreneurship programmes in tribal area.

Technologies Generated

1. Solar drying Technology: Production,preservation, processing, packaging methodsand quality parameters were standardized forsolar drying of fruit based food products viz.,Amla powder, amla candy, amla ginger, amlasupari, tooty fruity, copra powder and mangojelly.

2. Soya Milk Extraction: Production andprocessing technology of soya milk by usingsimple boiler method was developed in theproject. By use of heat inactivation of lipoxidaseand by removing the flavour compounds byevaporation (deodorization), masking thebitterness and off-flavour by sweetening andflavouring agents (chocolate and rose milkflavour), the milk can be readily consumed. Thetime and labour saving technology ofpreparation of Soya milk and milk basedproducts ( Tofu, granules, nuggets, meal maker)were popularized among ashram schools. Thetechnology was introduced in tribal Ashramschools and this is being consumed by the tribalchildren as protein substitute as the availabilityof cattle milk is difficult in this hilly area.

3. Millet based Products : Productiontechnology was standardized for millet basedproducts (biscuits, cakes and curry puffs)withvariety of combinations by using cereals, pulsesand oil seeds. These low cost supplements arepopularized in tribal area for benefitting ruraland tribal children below five years.

4. Herbal Powders: Production and processing

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

41 ICAR - CTRI Annual Report 2015-16

technology by using solar drying method wasstandardized for making triphala powder, neemleaf powder, neem seed kernel powder, rita nutpowder, mint leaf powder, curry leaf powderand henna powder. Varieties of mixtures werepopularized by taking these products as basematerial for consumption and commercialpurposes.

5. Weaning Mixtures: Production technologywas standardized for three varieties ofsupplementary weaning mixtures with varietyof combinations by using cereals (ragi,sama andkorra), pulses and oil seeds. These low cost dietswere developed by using the millets availablein tribal hamlets. They are popularized in ruraland tribal areas for the benefit of children belowfive years.

Development of Agro-technology for Sustainable Tobacco Production and Strengthening TOT

ICAR - CTRI Annual Report 2015-16 42

III. Identification of Alternative Crops andExploiting Tobacco for Alternative Uses

Crop productivity, soil quality and economicreturns under intercropping system (chewingtobacco +Annual moringa) in response tonutrient management.M. Kumaresan and D. Damodar reddy (CTRIRS, VEDASANDUR)

Annual moringa at different populationsviz., 1,00,75 and 50% was inter crop withchewing tobacco under 100% population with3 levels of fertilizers (75,100and 125% RDF) .The experiment was conducted in a split plotdesign.

The results of the 3rd year revealed thatthe different annual moringa population intercropped in chewing tobacco did not affect thefirst grade leaf yield (FGLY) or total cured leafyield (TCLY) of chewing tobacco. The fertilizerlevels viz., 75% 100% and 125 % RDF showed asignificant between the treatments. The FGLYand TCLY with 100% and 125% RDF arecomparable. The gross returns, net returns andB:C ratio were also not affected by the differentlevels of annual moringa planted in chewingtobacco. The different levels of fertilizerssignificantly influenced the gross returns, netreturns and B:C ratio of chewing tobacco . Thegross returns, net returns and B:C ratio withrespective 100% and 125% RDF werecomparable.

Soil residual fertility status after tobacco(2013-14)

The population of annual moringa andnutrient management in tobacco + annualmoringa inter-cropping did not influence theOC % at different depths. The OC % at 0 to22.5 cm ranged between 0.40 to 0.47% and at22.5 to 45 cm depth the OC% ranged between0.27 to 0.31%. The available P2O5 significantlyinfluenced annual moringa population. Annualmoringa at 50% population inter-cropped withchewing tobacco significantly increased theavailable P2O5 at both the depths. Differentlevels of RDF also influenced the available P2O5

status of the soil in both the depths. Theavailable K2O was influenced by the annualmoringa population at different fertility levelsat both the depths 0 to 22.5 and 22.5 to 45cm.The recommended dose of RDF at 125 %significantly increased the available K statusover the 75% RDF. The available K2O rangedbetween 346-394 in the 1st depth and in the 2nd

depth the K2O ranged between 351-403 kg/ha.

Tobacco+Moringa

Leaf Biomass Improvement in AdvancedBreeding Lines for Alternative Uses(CTRI,Rajahmundry)S.Kasturi Krishna, T. G. K Murthy, K. Siva Rajuand S. V. Krishna Reddy

Advanced breeding lines (HDBRG, GT-7xA-145, TI-163 X A-145, RT 46-1, RT 51-1), threespacings (60 X 40 cm , 70 X 40 cm, 80 x 40) andtwo fertiliser levels ( 100:50:50and 150:75:75kg NPK/ha) were tested in split plot design withthree replications. Observations taken duringcrop growth stage showed significant treatmentvariations with regard to plant height and no.ofleaves Line RT 51-1 recorded significantly higherplant height and no. of leaves followed byHDBRG for plant height and RT 46-1 for leafnumber. Regarding spacing, though significantdifferences were not observed betweentreatments 80x40 cm recorded higher plantheight and 70x40 recorded more no. of leaves.Significant differences were not observed forfertiliser doses.

43 ICAR - CTRI Annual Report 2015-16

Leaf Biomass: Regarding leaf yield HDBRGproduced significantly higher yield due to moreno.of leaves and HI. Line RT 51-1 thoughrecorded higher plant height, more no.ofleaves; lower leaf length, width and HI resultedin lower yields. Spacing 70x40 cm gave higheryields though no significant differences wereobserved (Table 1&2). Fertiliser dose of150:75:75 NPK kg/ha recorded higher leaf yieldthan lower dose. Interaction table showed thatHDBRG with 80 x40 spacing and 150:75:75 NPKkg/ha recorded higher leaf yields of 410.32 q/ha followed by 70 x40 spacing and 100:50:50NPK kg/ha line with an yield of 403.73 q/hacompared to other lines.

Nicotine Yield: Significant differences wereobserved in nicotine yield due to lines, spacingand fertiliser levels. Line RT 51-1 followed byTI-163 X A-145 line recorded significantly highernicotine. Wider spacing 80X 40cm recordedhigher nicotine content of 54.34 kg/ha followedby 70x40cm and 60x40 cm spacing. Fertiliserdose of 150:75:75 NPK kg/ha recorded highernicotine than lower dose. Interaction tableshows that Line RT 51-1 with a of 60 x40 spacingand 150:75:75 NPK kg/ha recorded higher

nicotine yields followed by TI-163 X A-145 linewith 80 x40 spacing and 150:75:75 NPK kg/ha.

Solanesol Yield: Significantly higher solanesolwas recorded by HDBRG of (39.07) than otherlines; Wider spacing of 80X 40 recorded highersolanesol followed by 70x40cm; Fertiliser doseof 150:75:75 NPK kg/ha recorded highersolanesol yield than lower dose. Interactiontable shows that HDBRG with a of 70 x40 spacingand 150:75:75 NPK kg/ha recorded highersolanesol yield of 55.10 kg/ha followed by sameline at 60x40 spacing with 100:50:50 NPK kg/ha.

Protein Yield: Significantly higher protein wasobserved in HDBRG (715.88kg/ha) than otherlines; spacing of 70X 40 cm recorded higherprotein; Fertiliser dose of 150:75:75 NPK kg/harecorded higher protein yield than lowerdose.Interaction table shows that HDBREG with70x40 spacing and 150:75:75 NPK kg/harecorded higher protein yield of 925.7 kg/hafollowed by same line at 60 x40 spacing with100:50:50 NPK kg/ha which was on par withline RT 46—1 at 60 x40 spacing with NPK kg/ha150:75:75 .

Identification of Alternative Crops and Exploiting Tobacco for Alternative Uses

ICAR - CTRI Annual Report 2015-16 44

IV. Management of Resource Constraints forProduction Efficiency and Product Quality

Identification of constraints and theirmanagement for effective utilisation of naturalresources is of paramount importance foroptimizing resource use in an agro-ecosystem.Resource management is one of the importantfactors that determine the product quality.

IV (A). Evaluation of soil fertility, water qualityand plant nutrition constraints fortobacco and their management

Assessment of leaf quality of FCV tobaccousing hyper – spectral remote sensing andgrowth parameters: [CTRI-RS, Kandukur]L.K. Prasad, M. Anuradha,M. Prabhakar andD. Damodar Reddy

Canopy and leaf spectral reflectance valuesof FCV tobacco were compared and correlatedwith N, P and K contents and growth parameterswhich showed prominent response withincreasing levels of nitrogen (120, 180, and 240kg/ha) and potassium levels (120 and 240 kg/ha).

Quality constituents of leaf: Leaf nitrogen(0.87-5.65 %) showed good positive correlationwith canopy spectral reflectance. Linearregression showed that leaf nitrogen contentof cured leaf had significant relationship withleaf nicotine content (0.59 to 1.91 %, R = 0.903,Rsqr = 0.816) indicating role of nitrogen insynthesis of nicotine. Leaf potassium contentvaried form 1.12-4.43 % at different nitrogenand potassium levels. Reducing sugars rangedbetween 7.81 - 26.3 %. Nicotine contentincreased with nitrogen up to 180 kg but suchlinear response was not noticed.

Sensitiv ity analysis of Hyper Spectralvariations in leaf and canopy spectrums:Responses to N and K variation in spectral bandsare significant. Sensitive bands were delineatedfor important quality parameters. Canopyreflectance of % total chlorophyll and % leafnitrogen at bud initiation stage had significantcorrelation at wavelength bands 724-729 nmand 350-365 nm (R=-0.62&0.64-0.65),respectively.

Sensitive bands for canopy spectralreflectance for leaf potassium were observedbetween 747–862 nm with a positive correlationco-efficient value of 0.53 (Fig.IV-1). Sensitiveanalysis of the leaf reflectance in relation tonicotine and reducing sugars showed asignificant correlation (Nicotine: R= - 0.75;Reducing sugars: R= 0.42).Identified sensitivebands for nicotine were 539-541 nm and 710nm and for reducing sugars were 631-692 nmand 1901-1970 nm (Fig.IV-2).

IV (B). Soil quality and nutrient use efficiencyin relation to input management.

Evaluation of Crop Residue and Wood Ashes -Effects on Soil Fertility and PotassiumNutrition of Tobacco [CTRI, Rajahmundry]D. Damodar Reddy, J.P. Bindu, S. KasturiKrishna, M. Mahadeva Swamy, L.K. Prasad andK. Nageswara Rao

Biomass ash addition effects on soil pH andK-fertility: The dynamics of soil pH and K-fertility of an Alfisol (Jeelugumilli) amended

Fig.IV-1.Canopy reflectance with % leafpotassium.

Fig.IV-2. Leaf spectral reflectance with %nicotine in cured leaf

45 ICAR - CTRI Annual Report 2015-16

with crop residue and wood ashes were assessedin a 90-day incubation experiment. Thetreatments for incubation experiment includedfour ashes viz., cotton stem ash (CSA), barnwood ash (BWA), tobacco stem ash (TSA) andmaize rind ash (MRA), and CaCO3 at three ratesof addition (0.05, 0.10 and 0.15% on w/w basis)and a control (no biomass ash). Sub-samples ofsoils were drawn 6 times at pre-decidedintervals (7, 15, 30, 45, 60 and 90 days) duringthe course of incubation and analyzed for soilpH and available K. Addition of biomass ashescaused a marked increase in soil pH over theno-ash control and thereby indicated thepotential of biomass ashes as liming materialfor acid soils. For all the biomass ashes and atall the application rates, soil pH tended todecrease with the progress of incubation time.The magnitude of soil pH rise was larger withthe increase in ash application rate. Theincrement in soil pH also differed betweendifferent biomass ashes and followed the order:CSA > BWA > TSA > MRA (Fig IV-3). The additionof biomass ashes brought out a distinct changein soil K fertility. All treatments with biomassashes enhanced the K availability in soil ascompared to the no-ash control throughout theincubation period. Irrespective of ash type,increasing rates of ash addition resulted ingreater increase in K availability. The biomassash induced increase in K availability wasdirectly related to biomass ash K concentrationand hence the amount of K added throughashes. Among the biomass ashes, the increasein K availability followed the order: TSA > MRA> CSA > BWA (FIGIV-2).

Biomass ashes effect on phosphorusadsorption of an acid Alfisol: The increase insoil pH as a result of biomass ash addition mightalso have indirect influence on behaviour ofnutrients like phosphorus. A laboratoryexperiment was, therefore, conducted to studythe phosphorus sorption behaviour of an acidAlfisol amended with different biomass ashes(Tobacco stem ash, TSA; Cotton stem ash, CSA;Pigeon pea stem ash, PSA; Coffee husk ash, CHA;and Maize rind ash, MRA) at the rate of 0.1 %(on w/w basis). Phosphorus adsorptionisotherms were prepared for soil samples thatwere treated with different biomass ashes andincubated for 30 days. In general, all the soils

receiving biomass ashes as amendments showeda marked increase in the P adsorption ascompared with the soil amended with no ashcontrol. Highest phosphorus adsorption wasrecorded for soil amended with pigeon pea stemash (PSA). The greater phosphorous adsorptionobserved for PSA amended soil could beattributed to the formation of calciumphosphate complexes favoured by higher Cacontent and large rise in soil pH resulting fromthis ash.

Efficiency of biomass ashes as sources of Ksupply for flue cured tobacco on Alfisols: Twofield experiments, one at CTRI-RS, Hunsur inKLS region under rainfed conditions (kharif2014) and another at CTRI-RS, Jeelugumilli inNLS region under irrigated conditions (rabi 2014-15), were conducted to evaluate differentbiomass ashes alone or enriched with SOP assources of K for flue- cured tobacco (Kanchan)grown on Alfisols. The treatments included 4biomass ashes alone (TSA, CSA, PSA and EWA atHunsur and CSA BWA, TSA, and MRA atJeelugumilli) and their 4 combinations with SOP(50:50 wt. basis), SOP alone and a no-ashcontrol. The 10 treatments in all for each fieldexperiment were tested in a RBD with 4replications. The results indicate that tobaccoresponds well to K application (100 kg K ha-1)on the K deficient Alfisols in both KLS and NLSconditions. Application of biomass ashes eitheralone or in combination with SOP (50% + 50%)on 100 kg K ha-1 equivalent basis caused asignificant increase in GLY, CLY and TGE leaf ascompared to the control. Tobacco cured leafyields obtained with different biomass ashesalone or their mixtures with SOP were at a parwith the yields recorded with standard SOPfertilizer. The relative yield of biomass ashtreatments indicating that the biomass ashescan serve as potential sources for Ksupplementation for FC tobacco on lighttextured Alfisols (Table 1). All the treatmentsreceiving K also resulted enhanced Kconcentration in leaf and increased K uptakeover the control, with the effect being moreconspicuous for KLS than for NLS. The qualityparameters viz., nicotine and reducing sugarsfor both X and L position leaves were notaffected by the biomass ashes alone or incombination with SOP.

Management of Resource Constraints for Production Efficiency and Product Quality

ICAR - CTRI Annual Report 2015-16 46

Management of Resource Constraints for Production Efficiency and Product Quality

Table 1: Yield efficiency parameters

Parameter Hunsur Jeelugumilli

Yield Response (%) 16.13-31.64 16.01-25.21Agronomic Efficiency( kg CL/ kg K) 1.56-3.06 2.87-4.52Relative Yield (%) 88-96 98-105

Fig. IV-3: Changes in soil pH as induced bydifferent biomass ashes at varying rates of

application

Fig. IV-4: Changes in soil K availability asinduced by different biomass ashes at varying

rates of application

47 ICAR - CTRI Annual Report 2015-16

Secondary nutrient deficiency effects ontobacco plant nutrition [CTRI, Rajahmundry]M. Anuradha, D. Damodar Reddy and K.Sivaraju [CTRI, Rajahmundry]

Secondary nutrient deficiency effects onpotassium nutrition:

Sand culture experiments were conductedto study the effect of single and multiplesecondary nutrient deficiencies under sufficientand deficient conditions of phosphorus on plantgrowth, development and nutrient uptake.Uniform tobacco seedlings of variety Kanchanwere planted in washed sand which is suppliedwith different nutrient combination treatmentsusing Hoagland solution. The main treatmentswere with and without phosphorus. In eachtreatment all combinations of secondarynutrient stresses (Ca, Mg, S, Ca & Mg, Ca & S,Mg & S and Ca, Mg & S) were imposed. Resultsrevealed that single and multiple nutrientsecondary nutrient deficiencies reduced growthcharacters, net photosynthetic rate andnutrient uptake. Total chlorophyll content wasreduced due to Mg, S deficiency and theircombination treatments.

Under nutrient stress the concentration ofanti-oxidative enzymes increased to counteractthe adverse effects of stress. The increase insuper-oxide dismutase activity is high under Sdeficiency and its combination treatments.Under no phosphorus condition the plant growthis less and omission of secondary nutrientsfurther reduced the growth and uptake ofnutrients and showed visual deficiencysymptoms.

Evaluation of organic and inorganic soilamendments to minimize nutrient leachinglosses and enhance nutrient use efficiencyunder NLS tobacco production system [CTRI,Rajahmundry]J. Poorna Bindu and D. Damodar Reddy [CTRI,Rajahmundry]

System operating conditions for tobaccostems were optimized and the conditions wereof 500 0C for 90 minutes with the recovery of40%.Biochar from tobacco stems ischaracterized by high content of total organiccarbon (80%), N (1.23%), P (0.78%), K (3.48%)and alkaline pH of 9.42.The cation exchangecapacity of tobacco stem biochar and synthetic

zeolite were 30 and 270 0C mol (p+) kg-1

respectively.

IV(C). Characterization of soil biota and useof biofertilisers

Tobacco (Nicotiana tabacum L.) and stemassisted green synthesis of silvernanoparticles and evaluation of itsantimicrobial activity against agriculturalplant pathogens [CTRI, Rajahmundry]D.V. Subhashini

Preparation of tobacco leaf extract

Fresh and young leaves (10g) of tobacco,collected from black soils of ICAR-CTRI Farm,Katheru, was washed thoroughly with distilledwater and cut into fine pieces. Leaves weresubsequently macerated slightly with the helpof mortar and pestle and transferred into a 500ml beaker containing 200ml double distilledwater and boiled for 10 minutes at 60oC. Thesolution was filtered using Whatmann No 1 filterpaper.

Synthesis of silver nanoparticles

For synthesis of silver nanoparticles, 10 mlof freshly prepared plant extract was added to190 ml of 1mM AgNO3 solution at roomtemperature. The incubation of the mixture wascarried out at 30oC in dark condition for 72hours. Characteristic red colour appeared afterincubation of the mixture for 72 hrs, due toreduction of colloidal silver. UV-Vis spectroscopyshowed absorption maxima of the color at 420nm. The average particle size (z-average) wasfound to be 10853.3nm and a PDI value of 1.884while zeta value was found to be -29.1mV witha peak area of 100% intensity. SEM analysisshowed that the silver nanoparticles synthesizedfrom N. tabacum leaf are spherical to irregularin shape.TEM study showed that the size variedfrom 26.8-36.8 nm (small sized nanoparticles)to 36.9-53.3 nm (large sized nanoparticles).

IV (D). Evaluation of tobacco leaf and productquality

Studies on chemical constituents responsiblefor smoke flavour in tobacco grown underdifferent agro-climatic zones [CTRI,Rajahmundry]K. Sivaraju, T.G.K. Murthy and D. DamodarReddy

Management of Resource Constraints for Production Efficiency and Product Quality

ICAR - CTRI Annual Report 2015-16 48

Management of Resource Constraints for Production Efficiency and Product Quality

Nine varieties of bidi tobacco wereobtained from Bidi tobacco research station,Anand. Sixty one neutral volatile aromacompounds (NVAC) were identified among the9 varieties of bidi tobacco. The NVAC identifiedin different tobacco were grouped in differentclasses based on their structure. The compoundsidentified were the degradation products ofcarotenoids, thunberganoids, neophytadiene,terpenoids, phenylalanine metabolitecompounds and Maillard reaction products.

Terpenoids: The total terpenoids content variedfrom 69.43 to 148.12 ppm among the varieties.The variety GT5 showed maximum content ofterpenoids (148.12 ppm) but it was at a parwith the varieties MRGTH-1 and GABT-11. Theimportant compounds identified among theterpenoids based on relative abundance arecaryophyllene oxide, sclareoloxide, isophytol,3-hydroxy solavetivone, epiglobulol, farnesylacetone, spathlenol, limonene dioxide, indole,geranyl acetone, linalool, nerolidol-epoxyacetate and 4-ketoisophorone. The varietyMRGTH-1 showed maximum and significantlyhigher content of geranyl acetone (31.84 ppm)followed by variety GABT-11 (26.61 ppm). TheSpathulenol content was maximum in thevariety GT7 (10.807 ppm) followed by A119. Thevariety GABT-1 showed significantly higherlevels of farnesyl acetate (30.74ppm) comparedto other varieties. Linalool content wasmaximum in the variety GT4 (1.193 ppm) andwas not detected in the varieties MRGTH-1 andABT-10. Isophytol content varied from 0.657 to1.347 ppm among the varieties. The varietyMRGTH-1 showed maximum isophytol content(1.347ppm) and it was at a par with varietiesGT4 and GT5.

Carotenoid related compounds (CRC): Thecarotenoid degradation compounds viz.,demascone, damascenone, ionones, fourisomers of megastigmatrienone,dihydroactinidiolide and 3-oxo-alpha-ionone,which are related to aroma were identified.The total CRC were maximum in the varietyGABT-11 and significantly higher than othervarieties. Megastigmatrienone content wassignificantly higher in the variety MRGTH-1(29.24 ppm) compared to other varieties. Thevarieties MRGTH1 and GABT-11 showed

significantly higher levels of individual CRCcompounds.

Thunberganoids and cembrenoids:Thunberganoids and cembrenoids contentranged from 20.147 to 80.97 ppm among thevarieties. The variety GT4 showed significantlyhigher content of Thunberganoids andcembrenoids (80.97 ppm) followed by GT5 andMRGTH1. Among the Thunberganoids andcembrenoids, the individual compounds presentin abundance are solanone, thunberganol,cembrene and duvatrienediol. Solanone contentwas significantly higher in the variety GT4(61.04 ppm) followed by variety MRGTH-1. Thethunbergol content varied from 1.38 to 25.76ppm among the 8 varieties. Neophytadienecontent varied from 20.17 to 75.99 ppm amongthe varieties . The variety MRGTH-1 showedsignificantly higher levels of neophytadiene(75.99 ppm).

Phenylalanine metabolite compounds (PMC):Phenylalanine metabolite compounds weremaximum in the variety GABT-11(3.72 ppm)followed by MRGTH-1 (3.443 ppm) and theywere at a par with variety GT4. Among thePMC, phenyl acetaldehyde and phenylethylalcohol were the major compounds identifiedbased on the quantity. Phenyl acetaldehydecontent varied from 0.423 to 1.62 ppm amongthe varieties. The variety GT4 showed maximumcontent of phenyl acetaldehyde (1.62 ppm) andit was at a par with the variety GT5 and GABT-11.

Maillard reaction products (MRP):Maillardreaction products varied from 0.09 to 1.22 ppmamong the varieties. The variety GT4 showedsignificantly higher content of MRP (0.567 ppm).Among the MRP, furfural was the majorcompound and it was significantly higher in thevariety GT4 and was at a par with the varietyMRGTH-1.

Fatty acid composition of bidi tobacco:Maximum of 9 fatty acids were recorded in allthe varieties. Among the nine fatty acids,palmitate, linoleate, lenolenate and oleatewere in maximum contents. The per centcomposition among the fatty acids showed thatthe linolenate> palmitate> linoleate> oleate>heptadeconate> myristate> pentadeconate >laurate.

49 ICAR - CTRI Annual Report 2015-16

V. Integrated Management of Biotic Stresses

V (A).Monitoring of Insect pests and Diseases

Survey for assessment of insect pest incidencein tobacco and tobacco based croppingsystems of CBS and SBS (CTRI RS, Guntur)P. Venkateswarlu

A survey was conducted for assessment ofinsect pest incidence in tobacco and tobaccobased cropping systems of CBS and SBS area.Six tobacco Auction Platfarms viz., Vellampalli1 & 2, Ongole 1 & 2, Tangutur and Kondepi wereselected for this study. The major crops coveredunder this area are tobacco, cotton, chillies,pigeon pea and chickpea.

Tobacco

Nursery survey covering 19 villages (7 in CBSand 12 in SBS) and 57 nurseries was conductedand data were recorded. Out of them, 28.6%nurseries were infested by tobacco caterpillar,Spodoptera litura in CBS and 25.0% in SBS.

In main field survey, 10 villages in CBS and15 in SBS were selected. Aphid, Myzusnicotianae, budworm, Helicoverpa armigera,caterpillar, Spodoptera litura and leaf curlcaused by whitefly, Bemecia tabaci wererecorded in both areas. Incidence of other pestswas negligible. The average infestations of thesepests in the infested fields were 6.1, 6.5, 7.2and 8.5% in CBS and 5.6, 5.4, 6.8 and 8.2% inSBS, respectively.

Survey for plant parasitic nematodesassociated with tobacco (CTRI RS, Hunsur)S. Ramakrishnan

FCV tobacco growing areas in KLS regionwere surveyed by collecting soil and rootsamples randomly from fields in different Taluksviz., HD Kote, Hunsur, Periyapatna and Arkalguduand were processed for enumeration of variousnematode populations. Results revealed thepresence of following plant parasitic nematodesviz., Meloidogyne spp, Rotylenchulusreniformis, Helcotylenchus spp, Pratylenchusspp and Tylenchus sp, associated with main fieldtobacco. Maximum mean population of root knot

nematodes were found in Periyapatna regionfollowed by Hunsur, Arkalgud and H.D.Kote.Reniform nematodes, though found in largenumbers in soil samples, they are not pathogenicto tobacco as compared to root knot nematodes.Plants revealed the presence of heavy multiplegalls with attached slimy egg mass in roots.

(B) Development of IPM technology

Bio-efficacy and field evaluation of newinsecticides against tobacco pests (CTRI,Rajahmundry)U.Sreedhar and S. Gunneswara Rao

A. Evaluation of new insecticides againstSpodoptera litura Fabricius in tobacconurseries

An experiment was conducted to evaluatenew insecticide chlorfluazuron 5.4 % EC for itsfield efficacy against tobacco caterpillar,S.litura in tobacco nurseries. The newinsecticide chlorfluazuron 5.4 EC @ 0.0075%,0.015% & 0.03% was evaluated along withnovaluron 10 EC @ 0.01%, lufenuron 5 EC @0.006% and emamectin benzoate 5 SG @ 0.0025%and untreated control in a replicated trial. At 2DAS emamectin benzoate 0.0025% recordedleast (5.63%) seedling damage followed bychlorfluazuron 0.03% (7.64) and novoluron 0.01%(8.30). The seedling damage in the treatmentsof emamectin benzoate 0.025% andchlorfluazuron 0.03% was ona par with eachother. At 4 and 8 DAS more or less similar trendwas observed.

B. Evaluation of new insecticides againsttobacco aphid, Myzus nicotianae Blackman onFCV tobacco

A bulk trial was conducted to evaluateFlonicamid 50 WG @ 0.02% and pymetrozine 50WG @ 0.02% against tobacco aphid, M.nicotianaeon FCV tobacco in comparison with untreatedcontrol. At 2 DAS flonicamid @ 0.02% recordedlowest aphid population (2.75/plant) followedby pymetrozine 0.02% (3.62/plant) comparedto untreated control (34.81/plant). From 4 DAScent per cent control of aphids was observed in

ICAR - CTRI Annual Report 2015-16 50

both the plots treated with flonicamid andpymetrozine upto 16 DAS. Where as in untreatedcontrol plot continuous build up of aphidpopulation was recorded from 38.54/plant at 4DAS to 62.53/plant at 16 DAS. The per centplants infested in untreated control plots was67.6% at the first priming. Highest cured leaf(3050 kg/ha), bright leaf (1196 kg/ha) and gradeindex (1650) was recorded in the plot treatedwith flonicamid 50 WG @ 0.02%, where as itwas 2990 kg/ha, 1196 kg/ha and 1650 in theplot treated with pymetrozine 50 WG @ 0.02%.The untreated control plot recorded 2356 kg/ha of green leaf, 840 kg/ha of bright leaf and agrade index of 1260.

Table V-1: Evaluation of new insecticidesagainst tobacco aphid Myzus nicotianeblackman in FCV tobacco

Treatment Mean aphids /plant Days after spray

2 4 8 16

Pymetrozine 3.62 1.00 1.00 1.0050 WG 0.02% (12.1) (0.00) (0.00) (0.00)

Flonicamid 2.8 1.00 1.00 1.0050 WG 0.02% (6.7) (0.0) (0.00) (0.0)

Control 34.8 38.5 39.1 62.5(1217) (1485) (1525) (3908)

CD (p=0.05) 1.46 1.54 1.74 2.43

Figures in the parenthesis are retransformedmeans

C. Evaluation of new insecticides againsttobacco whitefly Bemisia tabaci (Gennadius)on FCV tobacco

A bulk trial was conducted to evaluateInsecticides viz., Flonicamid 50 WG @ 0.02%,spiromesifen 240 SC @ 0.02%, pymetrozine 50WG @ 0.02% and diafenthiuron 50WP @ 0.05%against tobacco leaf curl virus vector B.tabacion FCV tobacco. The insecticides were appliedtwice at 10 and 25 days after planting. Resultsindicated that among the treatments,diafenthiuron 50 WP @ 0.05% recorded leastwhitefly population at all the observations. At

7 days after I spray (DAIS) the population (0.74)in diafenthiuron was on par with pymetrozine(0.81) and significantly less than all othertreatments. At 15 DAIIS the population indiafenthiuron (0.83) was least followed bypymetrozine (1.44) which remained on par withboth flonicamid (1.48) and spiromesifen (1.55).The data on leaf curl infected plants showedthat it was least in diafenthiuron (2.0%) followedby pymetrozine (2.4%), flonicamid (3.2%) andspiromesifen (3.6%), where as it was 12.4% inuntreated control plot. Pymetrozine treatedplot recorded highest cured leaf (3080 kg/ha),bright leaf (1200 kg/ha) and grade index (1726)followed by flonicamid 2986 kg, 1110 kg/ha and1660 respectively.

D. Filed efficacy of new insecticides againsttobacco caterpillar S.litura on FCV tobacco(i) New insecticides, chlorfenapyr 10 SC @0.01% and metaflumizone 22 SC @ 0.04% wereevaluated for their field efficacy in a bulk plottrial in comparison with untreated control. Theresults showed that chlorfenapyr 10 SC @ 0.01%was most effective in controlling the infestationof S.litura in FCV tobacco as shown by nodamage both in terms of per cent plantsinfested, mean no of leaves damaged as well asleaf area damaged. The plot treated withmetaflumizone 22 SC @ 0.04% recorded 4.4%plant infestation. The mean number of leavesdamaged were 1.24, 1.28 and 1.35 at 2, 4 and 8DAS, where as the leaf area damaged was 10.01,10.98 and 11.30% as against mean no. of leavesdamaged in untreated control plots (1.71, 3.82& 2.79) and the per cent leaf area damaged (19.24, 23.50 and 26.99%) at 2, 4 and 8 DAS. Inthe untreated control plot the infestation was25.6%.

(ii) Chlorfluazuron 5.4 EC @ 0.075%, 0.015% &0.03% was evaluated along with novaluron 10EC @ 0.01%, lufenuron5.4 EC @ 0.06% andemamectin benzoate 5SG @ 0.0025% along withuntreated control against S.litura on field cropof FCV tobacco. Results indicated thatemamectin benzoate @ 0.025% recorded leastnumber leaves damaged (1.00) followed bychlorfluazuron 0.03% at all the observations andremained on par with each other. At 4 DASemamectin benzoate 0.025% (1.00),chlorfluazuron 0.03% (1.09) remained on parwith each other while novoluron (1.21) remained

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51 ICAR - CTRI Annual Report 2015-16

Integrated Management of Biotic Stresses

on a par with lufenuron((1.23) as well aschlorfluazuron 0.03%. At 8 DAS emamectinbenzoate, chlorfluazuron 0.03% (1.12) andnovaluron 0.01% ( 1.24) remained on a parwith each other while novoluron 0.01% remainedon a par with lufenuron (1.32). At 15 DASemamectin benzoate and chlorfluazuron 0.03%while remaining on a par with each otherrecorded less number of leaves damaged thanall others.

Evaluation of insecticide applicationtechnology for effective spray coverage onFCV tobacco in NLS (CTRI- Research Station,Jeelugumilli)G.Raghupathi Rao, U Sreedhar and K.Nageswara Rao

1.Assessment of spray volume requirementsapplied through different sprayers on FCVtobacco at different growth stages

Spray fluid applied through high volumesprayers viz., compressed, knapsack and Hi-tech and two low volume sprayers viz.,motorized knapsack (MKS) and high pressureknapsack sprayers ( HPKS) were assessed at sixstages of the crop growth commenced from 20DAP and subsequent four stages at 15 daysinterval.

Spray fluid applied through knapsack andHi -tech sprayer at 30 DAP was low of 125-120l/ha respectively as against compressionsprayer- farmers method (170 l/ha ) followedby compression sprayer (145 1/ha) and itreduced the quantity of insecticide to an extentof 41 and 21 % over compression sprayer-farmers method, and compression, respectively.Further, Hi- tech sprayer reduced the operationtime to an extent of 28 and 11 % overcompression sprayer- farmers method andcompression, respectively. Spray fluid appliedthrough low volume sprayers viz, high pressureknapsack (HPKS) and motorized knapsacksprayer (MKS) was low of 100 and 110 l/ha,respectively as against Hi tech sprayer (120 l/ha) with saving of time to a tune of 55% over Hitech sprayer.

At 50 DAP compression sprayer- farmersmethod showed higher spray fluid (195 l/ha)followed by compression and knapsack sprayer

175 and 150 l/ha, respectively as against Hi-tech sprayer (142 l/ha). Hi- tech sprayerreduced the operation time to an extent of 15and 6% over compression sprayer- farmermethod, and compression, respectively. Itrevealed that up to 50 DAP by considering plantcanopy, quantity of spray fluid and insecticiderequirement, application through Hi-techsprayer was more economical over low volumesprayers HPKS and MKS.

At 65 DAP the spray fluid applied throughlow volume sprayers viz., HPKS and MKS waslow of 120 and 130 l/ha as against 175 l/ha withHi tech sprayer. Though the quantity ofinsecticide required through HPKS was high of27 per cent over Hi -tech, it showed saving ofapplication time to an extent of 54 % over Hitech sprayer.

As the crop growth was not in accordancewith age, the spray fluid requirements atdifferent growth stages are found to beinappropriate and valid conclusions could notbe drawn. Under such sub normal growthsituations, by considering quantity of insecticideand operators time, low volume sprayers arenot preferable.

Table V-2: Spray fluid requirement throughdifferent high volume sprayers

Age Spray fluid l/ha % saving ofof insecticide bythe using Hi techcrop over(DAP)

CS CS KS Hi CS CS KS(Fm) (Fm)

35 170 145 125 120 41 21 550 195 175 150 142 36 20 465 220 200 180 175 26 14 3

2.Spray characteristics as influenced bydifferent sprayers at 35 and 65 days afterplanting

Due to sub normal growth and poor canopy,the droplets deposited on photographic paperstrips on top, middle and bottom canopy werecoalesced and formed bigger droplets. As the

ICAR - CTRI Annual Report 2015-16 52

strips were completely flooded, the spraycharacteristics could not be analysed. Similarlydeposition of spray fluid on ground surface couldnot be determined.

3. Efficacy of different spray systems againstthe incidence of major insect pests of FCVtobacco

Incidence of leaf eating caterpillar,Spodoptera litura at 50 DAP was significantlylower to an extent of 11.4 per cent over control(18.4) in the plots treated through Hi- techsprayer. At 65 DAP the infestation wassignificantly low of 14.1 in the plots receivedinitial two sprayings through Hi -tech andsubsequent spray through HPKS and reducedthe infestation to a tune of 35 per cent overcontrol. At 80 DAP the infestation in differenttreatments ranged from 11.5 per cent in theplots received initial two sprayings through Hitech and subsequent two sprayings through HPKSto 14.9 through compression sprayer.

Incidence of aphid, Myzus nicotianae at 50DAP was significantly low of 12.8 in the plotstreated through Hi-tech and rest of thetreatments were at par. At 65 DAP, theinfestation was significantly low of 12.4 in thetreatments received initial two sprayingsthrough Hi tech and rest two through HPKSCritical perusal of data indicated that theinfestation was lowest of 13.1 in the plotsreceived initial two sprayings through Hi techand rest two through HPKS and superior overother treatments.Similarly Hi tech sprayershowed superior performance up to 50 DAP insuppressing i1Incidence of tobacco budworm,Helicoverpa armigera and there after HPKS wasmore effective in minimizing the pest incidenceon tobacco crop.

Spray fluid requirement during vegetativestage of the tobacco in NLS(Feeler trial)

At 20 DAP the spray fluid requirement variedfrom 60 l/ha ( nozzle discharge @ 250 ml/min)to 260 l/ha (nozzle discharge @ 1000 ml/min).Itvaried from 63 to 275 l/ha ,when the crop was35 DAP. Similarly at 40 DAP it varied from 65 to280 l/ha .. Further it is evident that the cropgrowth was not in accordance with the age, the

spray fluid requirements at different growthstages are found to be in appropriate. Dropletsdeposited on photographic papers could not beanalysed as the droplets coalesced and formedbigger droplets.

II - Influence of insecticide formulations,discharge rate and sprayers on the incidenceof major insect pests on FCV tobacco (CTRI-Rajahmundry)

It was evident that pplication ofimidacloprid 200 SL at a discharge rate of 450ml/min through Hi tech sprayer was effectivein minimizing the aphid infestation at 35 DAP.

Infestation due to , S. litura : At 45 DAPleaf eating caterpillar infestation was low of16.8 in the plots treated with novaluron 8.8 SCat a discharge rate of 450 ml/min appliedthrough Hi tech sprayer as against 22.7 incontrol.

Spray characteristics indicated that at 25days after planting(DAP), imidacloprid 200 SLemitted through Hi- tech sprayer at a dischargerate of 450 ml/min, 40 PSI pressure with awalking speed of 3.6 -4 kmph was characterizedby superior spray characteristics of higherdroplet density (67 per sq.cm), lower uniformcoefficient (1.51) and higher coverage (0.66)on plant canopy At 45 DAP spraying withnovaluron 8.8 SC, 450 ml/min through Hi techsprayer exhibited superior spray characteristicsof higher droplet density (62), lower uniformcoefficient (1.52) and high (0.65) coverage.

Management of ground beetle, Mesomorphusvilliger Blanchard in FCV tobacco (CTRI,Rajahmundry)U.Sreedhar

A replicated field trial was conducted witheight treatments viz., T1foliar spray (FS) ofimidacloprid 200 SL @ 0.005% on the seed bed 1day before transplanting, T2 Seedling rootdip-Imidacloprid 70 AF @ 0.14% before transplanting,T3 Imidaclorpid 200 SL 0.005% in transplantwater, T4 Foliar spray of Imidacloprid 200 SL0.005% a day after transplanting (DAT), T5- T2+ FS of Imidacloprid 200 SL 0.005% 5 DAT, T6- T1+ T5, T7- Neem cake application @ 5g/plant,T8- FS of tray seedlings 1 day beforetransplanting with imidacloprid 200 SL @ 0.005%T9- Untreated Control. Observations on plant

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53 ICAR - CTRI Annual Report 2015-16

mortality were recorded at 7,15&30 days afterplanting (DAP).

The results indicated that at 7 DAT leastper cent plant mortality of seedlings wasrecorded in T2-Seedling rootdip- Imidacloprid70 AF @ 0.14% before transplanting and T5- T2+ FS of Imidacloprid 200 SL 0.005% 5 DAT.However, T3 Imidaclorpid 200 SL 0.005% intransplant water (3.50) remained on par withthese two treatments as shown by per centtransplant mortality. These three treatmentsgave significantly higher protection than allother treatments. At 15 DAP T5- T2 + FS ofImidacloprid 200 SL 0.005% 5 DAT recorded centper cent protection and significantly superiorto all other treatments. T2 Seedling rootdip-Imidacloprid 70 AF @ 0.14% before transplanting(7.00), T3 Imidaclorpid 200 SL 0.005% intransplant water were the next best treatmentsand significantly superior to the rest. At 30 DAPsimilar trend was observed, however, T3 alsoremained on par with T 8 (17.10) and T 7(17.27%).

Data on yield parameters (83 b) showed thatT5- T2 + FS of Imidacloprid 200 SL 0.005% 5 DATrecorded highest cured leaf yield (2890 kg/ha)followed by T2 Seedling rootdip- Imidacloprid70 AF @ 0.14% before transplanting (2830kg) andT3 Imidaclorpid 200 SL 0.005% in transplantwater (2796 kg/ha) which were on par with allthe treatments except T1foliar spray (FS) ofimidacloprid 200 SL @ 0.005% on the seed bed 1day before transplanting and T4 Foliar spray ofImidaclorpid 200 SL 0.005% a day aftertransplanting (DAT) which recorded less curedleaf yield than other treatments. As regardsbright leaf yield T5 recorded highest (1300 kg/ha) followed by T2 (1270) and T3 (1200). Similartrend was observed for grade index except thatT8 remained ona par with the best treatmentsi.e., T5, T2 and T3.

Efficacy of new fungicides for themanagement of Frog eyespot disease inTobacco caused by Cercospora nicotianae(CTRI, Rajahmundry)U. Sreedhar and S. K. Dam

Five fungicides were evaluated against frog-eye leaf spot disease and the results revealedthat spraying with carbendazim @ 0.05 per centimmediately after appearance of the disease

followed by another spray at 10-15 days intervalrecorded minimum per cent disease index (PDI)of 19.92 which was on par with pyraclostrobin +metiram @ 0.2% (19.94 PDI) followed bykresoxim methyl @ 0.1 per cent (21.03 PDI).Among the fungicides propiconazole @ 0.1 percent (21.23 PDI) and azoxystrobin @ 0.1 per cent(21.24 PDI) did not perform better in checkingthe incidence of the disease. All were superiorto untreated check with more than 36 per centreduction of PDI over control. Maximum diseaseincidence was noticed in untreated control(31.36 PDI). There was no significant differencewith respect to cured leaf yield among differenttreatments tested. However, application ofpyraclostrobin + metiram recorded maximumgrade index of 892 followed by carbendazim 826and kresoxim methyl 778 when compared tocontrol.

Table V-3: Efficacy of different fungiciedes onfrog-eye leaf sport disease of FCV tobacco

Fungicides PDI % diseasereduction

overcontrol

Azoxystrobin 23% SC 21.24 33.09@ 0.1%

Propiconazole 25% EC 21.23 31.79@ 0.1%

Kresoxim methyl 44.3% 21.03 42.47SC @ 0.1%

Pyraclostrobin+Metiram 19.94 55.5660% WG @ 0.2%

Carbendazim 50% WP 19.92 55.79@ 0.05%

Untreated check 31.36

CD at 5% 0.51 -

Efficacy of new fungicides for themanagement of leaf blight disease in tobacconursery caused by Phytophthora parasitica f.sp. nicotianae (Breda de Haan) TuckerU. Sreedhar and S. K. Dam

In vitro studies

A total of seven fungicides were evaluatedin vitro against Phytophthora parasitica f. sp.

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ICAR - CTRI Annual Report 2015-16 54

nicotianae causing leaf blight disease of nurserytobacco by poisoned food technique at fourdifferent concentrations of 100, 250, 500 and1000 ppm. The results indicate thatout of sevenfungicides evaluated against the test pathogen,fenamidone + mancozeb was most effective asit checked 100% growth of fungus even at 100ppm followed by metalaxyl + mancozeb andpyraclostrobin + metiram at 1000 ppmconcentrations, respectively.

Nursery experiment

Attempts were made to find out theeffective fungicides for managing leaf blightdisease in FCV tobacco nursery. Seven fungicideswith two sprays (21st and 31st days after sowing)were tested along with untreated checkreplicated thrice on FCV tobacco nursery.Fenamidone + mancozeb followed by metalaxyl+ mancozeb , pyraclostrobin + metiram showedminimum disease index. Whereas, fenamidone+ mancozeb showed 100% disease reduction ascompared to control followed by metalaxyl +mancozeb 65% (positive check). Higher numbersof transplantable seedlings were recorded infenomidone + mancozeb (850/m2) @ 0.3% sprayfollowed by metalaxyl + mancozeb (796/m2) @0.2%. The Fungicidesdoes notshow anyphytotoxicity even at 3 days after spraying.

Table V-4: Efficacy of new fungicides onmanagement of leaf blight disease in FCVtobacco nursery

Fungicides Diseased %seedlings/ reduction

m2 of diseaseover

control

Azoxystrobin 23% 4.80 51.7SC @ 0.1%Trifloxystrobin + 5.35 35.4Tebuconazole75% WG @ 0.1%Pyraclostrobin + 3.29 60.3Metiram 60%WG @ 0.2%Kresoxim methyl 4.98 39.944.3% SC @ 0.1%Fenomidone + 0.00 100.0Mancozeb 60%WG @ 0.3%

Metalaxyl + 2.93 64.6Mancozeb 72%WP @ 0.2%Copper oxychloride 3.60 56.550% WP @ 0.2%Untreated check 8.28 -CD at 5% 1.61 -

Compatibility of recommended fungicides andinsecticides against Phytophthora parasiticaf. sp. nicotianae

Four fungicides viz., copper oxychloride0.2%, metalaxyl + mancozeb 0.2%, fenamidone+ mancozeb 0.3% and metalaxyl- m + mancozeb0.2% alone and in combination with tworecommended insecticides viz., emamectinbenzoate 0.05% and imidacloprid 0.25% weretested in vitro against Phytophthora parasiticaf. sp. nicotianae by poison food technique. Centper cent mycelial growth inhibition wasrecorded in fenamidone + mancozeb alone andin combination with emamectin benzoate andimidacloprid, followed by (metalaxyl +mancozeb) + imidacloprid (96.0%) and showedhigh compatibility. This indicated thecompatibility of all recommended fungicidesagainst Phytophthora parasitica f. sp. nicotianaewith the recommended insecticides.

Pythiumaphanidermatum

Five recommended fungicides in tobacconurseries viz., copper oxychloride 0.2%,metalaxyl + mancozeb 0.2%, fenamidone +mancozeb 0.3%, metalaxyl- m + mancozeb 0.2%and azoxystrobin 0.1% alone and in combinationwith two recommended insecticides viz.,emamectin benzoate 0.05%, imidacloprid 0.25%were tested in vitro againstPythiumaphanidermatum by poison foodtechnique. Cent per cent mycelia growthinhibition was recorded in combination of thetreatments i.e. (fenamidone + mancozeb) +emamectin benzoate, azoxystrobin + emamectinbenzoate and (metalaxyl + mancozeb) +imidacloprid followed by (metalaxyl- m +mancozeb) + ememectin benzoate (93.33%).Other fungicides and insecticides combinationwere also found inhibitory at their respectiverecommended doses and were superior overcontrol, thus indicated the compatibility of

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55 ICAR - CTRI Annual Report 2015-16

recommended fungicides against Pythiumaphanidermatum with insecticides.

Studies on Bio-ecology and management ofHelicoverpa armigera Hub.in tobacco as oilseed crop

An experiment was conducted to evaluatethree different modules viz., bio intensive IPM,chemical control and IPM in comparison withuntreated control for management ofHelicoverpa armigera Hub. in tobacco as oil seedcrop. Bidi tobacco variety A119 was planted inbulk plots of 500 plants each. Pheromone trapswere installed to monitor the activity of thepest. The components of BIPM module wereNSKS spray at flowering when the moths wereobserved in the trap. Two sprays of Ha NPV whenthe 1st and 2nd instar larvae were observed onthe inflorescence and the capsules. Thechemical control module consisted of threesprays of flubendiamide 480 SC @ 0.012% andchlorantranaliprole 25 SC @ 0.0075% atflowering, capsule formatioin and seed fillingstages. The components of IPM module werespray of NSKS and Ha NPV at flowering andcapsule formation, and flubendiamide 480 SC@ 0.012% need based spray of novaluron 10 [email protected]%.The capsule damage was least in chemicalcontrol module (4.50, 4.65, and 6.81%) at allthe observations and was on a par with IPMmodule (5.02, 5.13& 6.13) both of which weresignificantly superior to BIPM module (12.67,12.81& 17.72) at all the observations. The dataon percent plants infested with the pest alsoshowed similar trend. The activity of N.tenuiswas highest in untreated control plot followedby BIPM and IPM plots. The highest seed yieldof 560 kg/ha was recorded in chemical controlplot followed by IPM plot (546 kg/ha).

Validation of IPM module against tobaccoaphid, Myzusnicotianae under CBS conditions(CTRI RS, Guntur)P. Venkateswarlu

Bulk trial evaluation of identified IPMmodule in one acre area was carried out during2014-15 at CTRI Research Station, Guntur. Themodule consists of two rows of maize border asbarrier crop with one spray of Verticilliumlecanii @ 3X1012 CFU/ha at 50 DAP and one sprayof imidacloprid @ 50g a.i./ha (0.03%) at 60 DAP.

Chemical control plot (farmer practice) with onespray of imidacloprid at 50 DAP and one sprayof thiomethaxam (0.02%) at 60 DAP was keptfor comparison. An unsprayed plot with onlyborder crop and another unsprayed plot withoutany border crop were kept as controls.

A. Aphid population

All three treatments/modules weresignificantly superior over untreated control inreducing aphid population at 10 days of secondspray. IPM module exhibited 100% reduction ofaphid population on top leaves which was onpar with chemical control module (farmerpractice). Border crop alone also reduced aphidpopulation by 20.68% over untreated control.

B. Yield

As aphid infestation was low during theseason, there was no much difference in yieldamong all experimental plots. However,maximum yields of 13,380, 1,948 and 1,416 kg/ha of green, cured and bright leaf was recordedin chemical control module followed by bio-intensive IPM module with 13,345, 1,925, 1,405kg/ha and maize border plot with 13,317, 1,927and 1,380 kg/ha, respectively. There was anincrease of 0.68 to 1.88% total cured leaf wasrecorded in treatments over untreated control.

Integrated management of root knotnematodes and soil borne fungal diseases inFCV tobacco nursery (CTRI RS, Hunsur)S. Ramakrishnan

Experiments on integration of solarisationand chemicals with antagonistic organisms,Trichodermaviride, Paecilomyceslilacinus andPochaniachlamydosporia singly and in rationalcombinations against root knot nematodes andsoil borne fungal diseases revealed that,integrated application of Trichodermaviride&Paecilomyceslilacinus, Trichoderma viride andPochaniachlalmydosporia along with ridomil andfuradon in solarised nursery beds were on parwith each other in recording 50.0 and 45.1 percent increased healthy seedling count comparedto check. Similarly both the effective treatmentsrecorded decreased root knot index to the tuneof 49.2 and 48.7 percent respectively and alsodecreased damping-off + blight disease

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incidence in nursery beds to the tune of 57.14percent over untreated check.

Evaluation of bio-agents enriched trayseedlings against Root Knot Nematode-Fusarium wilt disease complex in FCV tobaccofield crop (CTRI RS, Hunsur)S. Ramakrishnan

The coco-peat media used for raising trayseedlings were fortified with bio-agents viz.,Trichodermaviride, Paecilomyceslilacinus andPochaniachlamydosporia singly and in rationalcombinations in scheduled dosage levels andwere evaluated against root knot nematodes andfusarium wilt disease incidence on FCV tobaccocrop raised in sick field conditions. Un-treatedtray seedlings and tray seedlings treated withcarbendazim and furadon were also used forcomparison and un-treated conventionalseedlings served as untreated check. Resultsrevealed that, T.viride (50g) + P.lilacinus (50g)enriched tray seedlings, T.viride (50g) + P.chlamydosporia (50g) enriched tray seedlingsand T. viride (30g) + P.lilacinus (30g) + P.chlamydosporia (30g) were on par with eachother in increasing the cured leaf yield by 10.0,9.5 and 10.5 per cent respectively over check.These effective treatments also decreased theroot knot index by 46.0, 49.1 and 51.0 per centrespectively and decreased the wilt diseaseincidence by 46.1, 42.3 and 51.3 per centrespectively over check. Whereas, the chemicalcontrol schedule, fuiradon + carbendazim at thetime of planting was found to be the best indecreasing the Fusarium wilt disease by 59.0per cent over check under field conditions.

Treatments Cured RKI % Wiltleaf diseaseyield incidence

(kg/ha)

T1 1473 1.92 25.3T2 1370 2.76 39.0T3 1377 2.52 21.3T4 1333 52.5CD at 5% 39.30 0.20 8.15

T1: Trichoderma viride @ 30g + Pochaniachlamydosporia @ 30g + Paecilomyceslilacinus @ 30g / tray media

T2: Un Treated tray seedlingsT3: Conventional seedling + Carbofuran @ 1g

/ plant+ carbendazim 0.2%T4: Untreated check

V (C). Screening for host plant resistance toinsect pests and diseasesScreening of tobacco germplasm against root-knot nematodes (CTRI RS, Hunsur)S. Ramakrishnan

A total of 30 advanced breeding linesmaintained at CTRI Research Station, Hunsurwere subjected to intensive screening againstroot-knot nematodes under sick field conditions.The varieties Rathna and Kanchan were includedas resistant susceptible checks. At maturity,the plants were uprooted and scored for Root-Knot Index (RKI) under 0-5 scale. The lines FCR28and FCJ22 recorded RKI 1.0 and were foundpromising against root knot nematode under sickfield conditions.

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57 ICAR - CTRI Annual Report 2015-16

On-farm Evaluation of Advanced BreedingLines in NLS region (CTRI, Rajahmundry)Y.Subbaiah, T.G.K.Murthy and K. Sarala

Evaluated the production potential,farmers’ acceptance and profitability ofadvanced breeding lines. On-farm trials wereconducted to evaluate the performance of ABLsTobios-2, 6 & 7; NLST-3, 4 & 5 with Kanchan ascheck in NLS area. Adopted all the goodagricultural practices in both the experimentaland control plots. Data were collected onincidence of pests and diseases, morphologicalcharacters, yield, quality, benefit-cost ratio andfarmers’ feedback.

Cured leaf yield: Higher cured leaf yield wasrecorded in Tobios-6 (2798 kg/ha) and Tobios-2(2633 kg/ha) at Gowravaram village. The yieldimprovement over check Kanchan (2403 kg/ha)was 16.44% in Tobios-6 and 9.57% in Tobios-2.At Nuthiramanna palem village NLST-3, 4 & 5were evaluated. NLST-4 (2104 kg/ha) hasoutscored the yield of check Kanchan (1922 kg/ha) by 9.47%. However, Tobios-7, NLST-3 & 5have not shown improvement over check. Dataon leaf quality parameters indicated that thereare no perceptible variations in leaf qualityparameters of ABLs and check cv., Kanchan.

From the benefit-cost ratio, it can beconcluded that ABL, Tobios-6 and Tobios-2recorded markedly higher BCR i.e. 1.47 & 1.42over Kanchan (1.35) at Gowravaram village. AtNuthiramanna Palem village, ABL NLST-4recorded superior BCR i.e. 1.23 over Kanchan(1.16). Farmers preferred ABLs Tobios-6 andNLST-4.

On Farm Demonstration and FrontlineDemonstrations (CTRI, Rajamundry)Y.Subbaiah

FLD on FCV tobacco variety TBST-2 in SLS,SBS & NBS areas

Selected the villages following the randomsampling procedure and farmers were selectedon purposive sampling basis covering SLS, SBSand NLS areas. Implemented FLDs through

Technology Assessed and Transferred

convergence approach and data were collectedon technical and socio-economic parameters

Yield, Quality and Economics

Superior cured leaf yield was recorded forTBST-2 over check, Siri in all the three regionsi.e. SLS (1677 kg/ha), SBS (2440 kg/ha) and NBS(3140 kg/ha). The yield improvement over thecheck Siri was 16.81%, 14.82% & 13.35% in SLS,SBS and NBS areas respectively maintaining thesame quality characters.

The results of benefit cost ratio, revealedthat ABL TBST-2 recorded markedly higher BCRin NBS (1.44), SBS (1.32) and SLS (1.11) ascompared to Siri i.e. NBS (1.36), SBS (1.22) andSLS (1.00). BCR was calculated on excludingthe imputed rental charges on land and barn.Good bodied leaf, required harvest intervals,TMV resistance and lower internodal length arethe advantages of TBST-2 over the control cv:Siri as opined by the farmers.

ARIS-15: Tobacco Agridaksh : An online ExpertSystemH Ravi Sankar

1) Home page of ‘Tobacco Agridaksh’ wascreated and linked to IASRI – agridaksh urlas: agridaksh.iasri.res.in.

2) Created a Knowledge model with attributesviz., basic information of CTRI, origin of thetobacco crop, package of practices, tobaccovarieties, success stories, nurserymanagement, production technology andcrop protection. Hyperlinks were providedfrom home page of tobacco agridaksh tothese attributes and related informationwas uploaded in IASRI server.

3) Created a knowledge acquisition modulewith various entities viz., crop varieties,diseases, insect – pests, weeds and the basicdata.

4) Developed knowledge base system fortobacco diseases as a 1) retrieval systems2) ontology based system for identification

ICAR - CTRI Annual Report 2015-16 58

of diseases useful for the researchers andextension agencies working on tobacco.

TRIBAL SUB PLAN

ICAR-CTRI : Manchulavarigudem, a tribal hamletof Seethappagudem Gram panchayat, WestGodavari district, Andhra Pradesh was selectedfor implementation of tribal sub-plan during2015-16. The following interventions wereimplemented for a total number of 38 farmfamilies.

● Supply of foundation seed of high yieldingmaize hybrid (DH-117) to improve the maizeyields (16 no.)

● Supplied fertilisers for balanced fertilisationof maize (16 no.)

● Supplied one weighing balance for properweighing of agriculture produce

● Grain storage bins were constructed (6 no.)to reduce the grain storage losses

● Improved sprayers (2 no.) were supplied forefficient pest and disease management

● Training programmes were conducted ongood agricultural practices of major crops(Paddy, maize, cotton and tobacco)

AINPT Centre, Nandyal, Andhra Pradesh

● TSP programme (2015-16) was implementedby AINPT Centre at RARS, ANGRAU, Nandyal.For the programme, about 40 tribal farmersof Sugalimetta village of Panyam Mandalwere selected. The following interventionswere implemented

● Identified critical inputs viz., Fertilizers,pesticides and improved varieties weredistributed to the farmers in order toimprove crop productivity.

● Training programme on suitable farmingmethods for increasing productivity levelsin Millets and Pulses was also organized forthe benefit of farmers.

● Relevant literature on the aspect wasprepared and distributed.

Grain storage structures, TSP-CTRI

Improved sprayers, TSP-CTRI

Supply of critical inputs for maize, TSP-CTRI

TSP AINPT Centre, Nandyal

Technology Assessed and Transferred

59 ICAR - CTRI Annual Report 2015-16

Education and Training

Different extension activities viz.., trainingprogrammes, scientist-farmer interfacemeetings, diagnostic visits, field days, kisanmelas, exhibitions, workshops and groupmeetings. Added emphasis has been accordedfor collaborative activities with Tobacco Board,Tobacco Industry and other relevantorganizations to achieve enhanced productivity,quality and profitability. The followingprogrammes were conducted during the periodunder report.

✦ Training to farmers 8✦ Training to ARS scientist trainees 1✦ Field visits 4✦ Diagnostic visits 4✦ Interactive meetings 2✦ Farmer discussions 2✦ Field Day 1✦ Field Friends Programmes 39✦ Demonstrations 15✦ Scientist Farmer Interface meetings 4✦ Radio talk 1✦ Students visits 7✦ Visitor Services 6

● Training programme on GAP in tobacco wasconducted for Tobacco Board FieldAssistants at CTRI RS Hunsur on 16.03.2015.

● Training on tobacco nursery managementwas conducted to FCV tobacco growers inassociation with Krsihi Gyana VigyanaVedike, Mysore on 18.03.2015 at Hunsur

● A Seminar on customized fertilizers fortobacco was organized by M/s ITC Ltd. andNagarjuna fertilizers on 16.4.15 at ICAR-CTRI, Rajahmundry.

● One day training programme on GAP andPHPM was conducted to the contact farmersgroups from different auction platform ofKLS at CTRI research Station Hunsur on16.6.2015

● One day training programme on GAP andPHPM was conducted to Tobacco Board FieldOfficers of KLS at CTRI research stationHunsur on 17.6.2015

● A training programme on Burley tobaccoproduction technology was conducted forGPI trainees from 21.9.15 to 23.9.15 atICAR-CTRI, Rajahmundry

● A training programme on “Good agriculturalpractices of FCV tobacco cultivation” wasconducted for newly recruited /promoted‘Field Assistants’ of Tobacco Board from 15-17 March, 2016 at CTRI, Rajahmundry.

Training on GAP to Tobacco Board officials

Krishi Unnati Mela: Participated and put up anexhibition stall in Krishi Unnati-NationalAgricultural Fair organized by Ministry ofAgriculture and Farmers Welfare during 19th to21st March, 2015 conducted at Mela Ground ofthe Indian Agricultural Research Institute, NewDelhi.

Awareness on PPVFR Act 2001: OrganizedTraining-cum-Awareness Programme on“Protection of Plant Varieties and Farmers’Rights Act” on 5th December 2015 at KVK,Kalavacharla and on 29th March 2016 at KrishiBhavan, Kakinada. Progressive farmers,Agricultural officers, Horticultural officers, ATMAfield staff (Block technology managers andSMSs), Multipurpose Extension officers, Faculty

ICAR - CTRI Annual Report 2015-16 60

and final year students of Agricultural College(ANGRAU), Rajahmundry, Scientists andtechnical staff of ICAR-CTRI, Representatives ofNGOs (World Vision) participated in theseprogrammes.

Fields friends programme

● The Scientists and Technical Officers ofCTRI, Rajahmundry and Research Stations,Guntur, Kandukur were nominated in theField Friends Teams as resource persons,being implemented by the Tobacco Board,Guntur during 2015-16 Crop season inAndhra Pradesh. The teams along withTobacco Board Officers and Executives fromthe trade visited the tobacco nursery areasand farmers’ main fields and advised GoodAgricultural Practices. The Field Friendsprogramme was implemented in thedifferent platforms viz., Devarapalli,Gopalapuram, Thorredu, Koyyalagudem,Jangareddygudem-I & II, Ongole-I&II,Vellampalli- I & II Tangutur I & II , Kondepi,Podili – I & II, Kandukur – I & II, Kaligiri andD.C. Palli of West Godavari, East Godavari,Prakasm & Nellore districts of AndhraPradesh.

Training-cum-Awareness Programme on PPVFR

Education and Training

61 ICAR - CTRI Annual Report 2015-16

S.N. Resource person Training imparted Date and place

1 S. Ramesh FCV tobacco nursery 21.05.2015 at M.M. Koppalu,management KLS

2 S. Ramesh FCV tobacco nursery 22.05.2015 atThandremanagement Ankanahalli, KLS

3 Scientists/ Fertilizer management 27.05.2015 at CTRI RS Hunsur inTechnical officers in field crop/reduction collaboration with Coromandel

of low grades international Ltd,

5 S. Ramesh Orientation on Good 05.06.2015 at CTRI RS,HunsurAgricultural Practices oftobacco cultivation forUG students of forestrycollege, Ponnampet

6 Dr.M.Mahadevaswamy Field crop management 08.06.2015 at HD Kote,of FCV tobacco KLS

7 S.Ramesh Field crop management 09.06.2015 at Thattakere,of FCV tobacco KLS

8 Scientists/ Training to progressive 16.06.2015 at CTRI RS HunsurTechnical officers farmers (120 numbers )

on FCV crop productiontechnologies

9 Scientists/ Training to field officers 17.06.2015 at CTRI RS,HunsurTechnical officers of Tobacco Board (29)

on FCV crop productiontechnology in KLS

10 Dr.S.Ramakrishnan Krishi mela organized 19.06.2015 at CTRI RS, Hunsurby Department ofAgriculture, Karnataka

11 Dr. S.Ramakrishnan Field crop management 20.06.2015 at APF-06, KLSto the FCV tobaccogrowers

12 Dr. S.Ramakrishnan Field crop management 23.06.2015 at APF- 64, KLSto the FCV tobaccogrowers

13 Dr. M. Mahadevaswamy Field crop management 24.06.2015 at APF – 6, KLSto FCV tobacco growers

14 Sri.S.Ramesh Field crop management 29.06.2015 at Heggandur,to FCV tobacco KLS

30.06.2015 to Periyapatna,KLS

Education and Training

ICAR - CTRI Annual Report 2015-16 62

S.N. Resource person Training imparted Date and place

15 Dr.S.Ramakrishnan Field crop management 06.07.2015 at APF 3, KLSto FCV tobacco growers

16 Sri.S.Ramesh Field crop management 15.07.2015 at Periyapatna,& crop protection agents KLS

17 Dr.M.Mahadevaswamy Field Crop management 16.07.2015 at HD Kote,& crop protection agents KLS

18 Sri.S.Ramesh Field Crop management 16.07.2015 at BTM Koppalu,& crop protection agents HD Kote, KLS

19 Dr.C.Mahadeva Field crop management, 17.07.2015 at Gangur.PHPM and crop Mullur, KLSprotection agents

20 Sri.S.Ramesh and Field crop management, 21.07.2015 at Hosaveedu,Dr.S.Sreenivas PHPM and crop B.D.Pura, Naganahally,

protection agents HD Kote, KLS

21 Dr.S.Ramakrishnan Workshop on Post 21.07.2015 at Periyapatna,Dr.M.Mahadevaswamy harvest product KLS

management in FCVtobacco organized byTobacco Board and trade

22 Dr.M.Mahadevaswamy Field crop 25.07.2015 at Hagaranallimanagement and Post and Manti koppalu,harvest product KLSmanagement

23 Dr.C.Mahadeva Field crop 28.07.2015 at APF 07 and 64,management and Post KLSharvest productmanagement

24 Dr.C.Mahadeva Field crop 29.07.2015 at Agrahara, KLSmanagement and Postharvest productmanagement

25 Sri.S.Ramesh Field crop 31.07.2015 at APF 07 and 64,management and Post KLSharvest productmanagement

26 Dr.S.Ramakrishnan Workshop on Field crop 07.08.2015 at Chilkunda,Dr.M.Mahadevaswamy management and PHPM Periyapatna, KLSS.Ramesh organized under core

committee

Education and Training

63 ICAR - CTRI Annual Report 2015-16

S.N. Resource person Training imparted Date and place

27 Dr. S. Ramakrishnan, Field crop 11.08.2015 at HD Kote,Dr. M. Mahadevaswamy management and Post KLS

harvest productmanagement

28 Sri I. Jagadish Chandra Nursery management 4.9.2015 at Sangayagudem, NLS

Dr. S.K. Dam Tobacco nursery 9.9.2015 at Koyyalagudemmanagement

29 Dr. S.K.Dam Nursery management 10-09-2015 at Koyyalagudem, NLS

30 Sri I. Jagadish Chandra Nursery management 11.9.2015 at Reddy Ganapavaram,NLS

31 Dr. S.K.Dam Nursery management 14.09.2015 at Gopalpuram, NLS

I. Jagadish Chandra Tobacco nursery 16.9.2015 at Thorredumanagement,preparation of nurserybuds, raising of tobaccoseedlings in poly traysand pest and diseasemanagement

I. Jagadish Chandra Tobacco plantings and 20.10.2015 at Devarapalli,manurial applications Badapuram

32 Dr. S.V. Krishna Reddy Main field preparation, 28.10.2015 at Koyyalagudem,fertiliser application, NLSridge formation,plantings,intercultivation, IPMand irrigation

N. Arunakumari Main field preparation, 29.10.2015 at Gopalapuram,plantings, fertilizer Chinnayigudemapplication, ridgeformation etc.

33 Sri I. Jagadish Chandra Transplantations, 5.11.2015 at Devarapalli, NLSfertiliser application,intercultivation,irrigations and IPM intobacco cultivation

34 Dr. S. Ramakrishnan Post harvest Product 06.11.2015 at Kellur village,Sri.S.Ramesh management KLS

35 S.Ramesh Post harvest Product 13.11.2015 at Motta,management KLS

Education and Training

ICAR - CTRI Annual Report 2015-16 64

S.N. Resource person Training imparted Date and place

36 Dr. P. Venkateswarlu Nursery management 12.11.2015 atand field preparation Nagabhiruvaripalem villagein SBS

37 Dr. P. Venkateswarlu Field day on VT-1158 09.02.2016 at Gundlapallivariety (OFT plot)

38 Dr.P. Venkateswarlu Field day on N-98 10.02.2016 at B.Nidamanuruvariety (OFT plot)

39 Dr. P. Venkateswarlu Field day on TBST-2 16.02.2016 at Ongolevariety (OFT plot)

40 Dr. P. Venkateswarlu Field day on TBST-2 18.02.2016 at Vemulapaduvariety (OFT plot) village

41 S.K. Dam Growers awareness 10.03.2016 at ICAR- CTRI RS,meeting on “Good JeelugumilliAgricultural practices,crop protection agentsand PHPM in tobaccocultivation”

Dr. K. Suman Kalyani Topping, desuckering, 11.01.2016 atorobanche management,brown spot diseasemanagement

Dr. K. Suman Kalyani Post harvest product 20.01.2015 atmanagement, bettercuring techniques

Dr. K. Suman Kalyani Grading and curing 29.02.2015 atmethods

Education and Training

65 ICAR - CTRI Annual Report 2015-16

RADIO TALKS

Sl. Name Topic Station; date ofNo. broadcasting

1. Dr. B. John Babu Potti mekala pempakam 14.04.2015, AIR,Vijayawada

2. Dr. P.V.V. Siva Rao Samvatsaram poduguna nanyamaina 17.04.2015, AIR,pasugrasala sagu Vijayawada

3. Dr. P. Venkateswarlu Plant protection equipment and precautions 29.04. 2015, AIR,while using pesticides through Vijayawada.

4. Smt. J.V.R. Satyavani Improved cultural practices and nutrients 04.05.2.15, AIR,management in mango and cashew orchards Vijayawadaduring flowering and fruiting stage

5. Dr. A.V.S.R. Swamy Dakshinadi telika nelalaku mariyu nalla 26.06.2015, AIR,regadi nelalaku anuvaina pogaku vangadalu Vijayawada

6. Sri R. Sudhakar Uses of Banana Pseudostems 03.07.2015, AIR,Vijayawada

7. Dr.M.Kumaresan Tobacco cultivation aspects 22.09.2015, AIR,Madurai

8. Sri.R.Rajendran Pest and Disease and their control measures 28.09.2015, AIR,in Tobacco Madurai

9. Sri R. Sudhakar Uses of Banana Pseudostems 23.10.2015, AIR,Visakhapatnam

10. Smt. V.V. L. Kumari Aarogya parirakshanalo chirudanyala 22.10.2015, AIR,pramukyatha viluva aadharitha padardhala Visakhapatnamtayari

11. Smt. J.V.R. Satyavani Jeedimamidi thotalalo adhika digubadulaku 14.11.2015, AIR,patinchavalasina yajamanya padhatulu – Visakhapatnammuduru thotallo punarudharana

Education and Training

ICAR - CTRI Annual Report 2015-16 66

Education and Training

Field visits

● Dr. S. Ramakrishnan and RM Tobacco Boardvisited the Sundavalu, R Thunga, CHP Halliareas of the Periyapatna Auction floor ofKLS on 01.06.2015.

● Dr. S. Ramakrishnan Dr. M. Mahadevaswamyvisited the tobacco growing areas inPeriyapatna on 30.06.2015 in KLS.

● Dr. S.Sreenivas visited the tobacco growingareas of Hosaveedu, B.D.Pura, Naganahally,HD Kote areas of KLS on 21.07.2015.

● Dr. S. Ramakrishnan Dr.M. Mahadevaswamyvisited the tobacco growing areas ofPeriyapatna along with Director ofAuctions, Tobacco Board Bangalore on04.08.2015.

● Dr. M Anuradha, Dr. K China Chenchaiah andDr. PV Venugopala Rao visited the on farmvarietal plots of TBST-2 at Chavatapalem –Kandukur-I plat form of SLS on 16.12.15.

● Dr. S. Kasturi Krishna, Dr. C. Chandra ShekarRao, Dr. K. Suman Kalyani and Dr. G.

Raghupathi Rao visited NLS area on21.12.2015 and monitored the cropssituation and advised the farmers about themeasures to be taken for the control ofprevailing pests and diseases.

● Dr. M. Kumaresan and Sri.R.Rajendranvisited tobacco nursery growing areas inTamil Nadu on 25.09.2015, 05.10.2015 and16.10.2015 and on-farm varietal trial plotson 18.12.2015.

● A survey on Orobanche was conducted inPrakasam and Nellore Districts (14 Platfarms) by Scientists of CTRI, Managers andAuction Superintendants of Tobacco Boardfor 5 days i.e. 21st, 22nd, 27th, 28th and29th January, 2016. The extent of damagevaries from 5 to 10%, approximately.Farmers were educated about Orobancheinfestation, its nature of damage, andmanagement practices through field visitsand meetings.

● Dr. K China Chenchaiah visited OFT plot ofN-98 at Ponnaluru- Kandukur-I on 22.01.16

67 ICAR - CTRI Annual Report 2015-16

Krishi Vigyan Kendra

Kalavacharla

ACHIEVEMENTS

● A total number of 6 On-Farm Testings (OFTs)and 10 Front-line Demonstrations (FLDs)were conducted during the year 2015-16.

● Pre emergence application of Pendimethalin@ 2.5 l/ha at 3DAS and post emergenceapplication of Quizalofop ethyl @1l/ha andPyrithiobac sodium @625 ml/ha at 25-30 DASeffectively managed the weeds in cotton.

● Application of sulphate of potash @100 kg/ ha in equal split doses one at 10 DAP andthe second one at 25 DAP resulted a tobaccoleaf yield of 20q/ha as against 16 q/ha incontrol

● Tissue culture Coccinia was introduced invirus prone Coccinia belt of East GodavariDistrict. No incidence of virus observed inexisting crop and 13 tonnes/ha yield wasrecorded compared to traditional variety(8.5 tonnes/ha).

● Introduced ‘Maran’ Ginger variety inirrigated areas which yielded 16.5 t/hacompared to local ginger of 12 t/ha.

● Bait formulation of Jaggery slurry (Jaggery6 kg in 12 litres of water) + Dichlorovas 250ml. + 1 kg wheat flour effectively cotrolledthe (23% mortality of snails).

● Introduced ‘Osmanabadi’ goats in EastGodavari District for both for meat and milk.Males grow upto 34 kg and females upto 32kg.

● Popularized Black Bengal goats in thedistrict and supplied 11 units (Each unit isof two females and one male) to the farmersthrough ATMA, (East Godavari) under FLDprogramme.

● Demonstrated Azolla, a cheap source ofprotein, as supplementary feed to poultrywith saving of feed cost by Rs.2/- per kg.

● Ovosync plus technology was implementedin cattle for inducing conception and 40%conception rate was noticed.

● Demonstrated fodder cluster bean varietiesBundel Guar-1 and Bundel Guar-2.

● Promoted the establishment of twopalmyrah fibre extraction units atSirsinapalli and Tulasipakala villages ofChinthuru Mandal.

● Promoted the establishment of one bananafibre production at Mandapeta.

● Promoted the establishment of one unit ofCup and Plate making with Bamboo culmsheath at Sirsinapalli village of Chinthurumandal.

Collaborative training Programmes

Demonstrations on Cashew AppleUtilization: Demonstrations on Cashew AppleUtilization were organised at KVK, Kalavacharlaon 13th, 14th, 15th and 16th May, 2015, incollaboration with Directorate of Cashewnut andCocoa Development (DCCD), Cochin.Fourbatches of tribal women and youth (100 nos.)from Rasthakuntuhai (Vizianagaram Dt.),Rajanagaram, Gandepalli, Murari,Punyakshetram and Kalavacharla villages (EastGodavari Dt) were trained in preparation ofvarious recipes of cashew apple viz.,cashewapple jam, juice, chutney, pickle and chips.

KisanMela: In collaboration with ATMA-EastGodavari district, KVK organised ‘KisanMela’ on9 November 2015 at KVK, Kalavacharla.Dr.D.Damodar Reddy, Director, CTRI,Rajahmundry presided over the function.

Distribution of Black Bengal goats to farmers

ICAR - CTRI Annual Report 2015-16 68

Krishi Vigyan Kendra, Kalavacharla

Hon’ble Sri PendurthiVenkatesh, MLA,(Rajanagaram, East Godavari District) gracedthe occasion as Chief Guest. A total no of 300farmers from 10 blocks of the East Godavaridistrict participated in the programme.‘Exhibition stalls’ were arranged displayingimproved technologies in agriculture,horticulture and allied sectors. Black Bengalgoats were distributed to farmers with thefinancial assistance from ATMA, East Godavari.

Kisan Sammelan: Organized Kisan sammelan/goshti on Rabi Vegetable cultivation on 1stMarch2016 at Madiki village of Alamuru mandal, EastGodavari District.Organized Kisan sammelan/goshtion Pulses Production on 30th March 2016at CTRI, Rajahmundry.

Training and Front line demonstrations onMilky Mushroom production

1. Participation in Rytu Sadbhavana Yatra:Participated in Rythu Sadbhavana Yatraalong with Scientists from Indian Instituteof Rice Research and ICRISAT on 27th January,2016

Internship: Eighteen RAWEP students of Finalyear B. Sc (Ag) , Agrl College, Rajahmundry didtheir internship during 4-22 December 2015 atKVK as a part of RAWE Programme.

Krishi Vigyan Kendra, Kandukur

Front line demonstration of FCV tobaccovariety TBST-2: Demonstration were conductedin farmer’s field with FCV tobacco variety TBST

2, a newly identified FCV tobacco line forprakasam and Nellore districts of AP

Popularisation of Napier grass variety CO-4cuttings to farmers: A fodder block (40 cents)area is maintained with CO - 4 grass and suppliedthe cuttings to needy farmers.

Front line demonstrations and Trainingprogramme on Milky Mushroom production:FLD’s were conducted on Milky mushroomproduction at Oguru village. A Trainingprogramme was organised on Milky mushroomproduction for two days where in 50 memberswere participated.

Front line demonstration with TBST-2

Rytu Sadbhavana Yatra

Crop Cafeteria: Different rainfed crops viz.,Sorghum, Bajra, Variga, Ragi, Korra, Blackgram,Green gram, Sunhemmp for seed, cowpea, horsegram, bengal gram, lentil, castor, lineseed,mustard, sesamum were grown to evaluate theirperformance and found that performance ofbajra, sorghum, variga, horse gram, bengal gramfound better compared to other crops.

69 ICAR - CTRI Annual Report 2015-16

Awards and Recognitions

● Dr. K. Suman Kalyani, Principal Scientist,ICAR- CTRI has been honoured with theprestigious ‘Bio-Technology SocialDevelopment Award – 2015’ by theDepartment of Bio- Technology, New Delhion 05.02.2016 during the GlobalBiotechnology Summit-2016 held at VigyanBhawan, New Delhi for her outstandingextension work in the area of diversificationof agriculture towards socio economic up-liftment of the grass root level rural andtribal farmers and farm women. The awardconsists of 3.00 lakh cash prize, citation,memento and a certificate. The award waspresented by Hon’ble Minister of Scienceand Technology, Dr. Harsh Vardhan.

● Dr. D. Damodar Reddy, Director, ICAR-Central Tobacco Research Institute,Rajahmundry received FELLOW OF ANDHRAPRADESH AKADEMI OF SCIENCES for hisoutstanding contributions to Science &Technology in the first induction ceremonyof ANDHRA PRADESH AKADEMI OF SCIENCESheld at Acharya Nagarjuna University,Guntur on 19th March, 2016. In thisconnection, he delivered a lecture on“Phosphorus use efficiency in IndianAgriculture – importance, impediments andimprovement interventions” to thedelegates.

Photo, Dr. D. Damodar Reddy

● H. Ravi Shankar Senior Scientist, ICAR-Central Tobacco Research Institute,Rajahmundry receivedOutstanding ScientistAward from Venus International ResearchFoundation Research Academy (VIFRA),2015

● Dr. H. Ravisankar, Senior Scientist, ICAR-Central Tobacco Research Institute,Rajahmundry received ASSOCIATE FELLOWOF ANDHRA PRADESH AKADEMI OF SCIENCESfor his outstanding contributions to Science& Technology in the first inductionceremony of ANDHRA PRADESH AKADEMI OFSCIENCES held at Acharya NagarjunaUniversity, Guntur on 19th March, 2016.

ICAR - CTRI Annual Report 2015-16 70

Strong linkages were developed with variousorganisations at regional, national andinternational level. At regional level, linkagebetween CTRI and various state governmentdepartments and Agricultural/ HorticulturalUniversities in Andhra Pradesh, Tamil Nadu,Karnataka, Bihar, Gujarat , Odisha and WestBengal was established to provide an effectivethrust to Indian tobacco development. Central

Linkages and Collaborations

Sl. Name of the Collaborating Agency ActivityNo.

a) National Institutes/Agricultural Universities

1 Tobacco Board, Guntur On Farm trials, Front line demonstrations andtraining programmes and Diagnostic visits

2 Bureau of Indian Standards, Development of Indian standards for tobacco andNew Delhi tobacco products

3 Department of Biotechnology, Empowerment of tribal’s through agro-ecologicalNew Delhi conservation and bio-technological approaches in

East Godavari district of Andhra Pradesh

4 National Bureau of Plant National Active Germplasm Site (NAGS). Import ofGenetic Resources, New Delhi tobacco germpalsm and maintenance

5 Department of Agriculture in Development of technologies related to differentdifferent states tobacco types and technology dissemination

6 Indian Meteorology Dept., Pune Maintenance of meteorological observatories atdifferent Stations

7 M/s ITC Ltd. ABD-ILTD Research and development activities andM/s. Godfrey Phillips India Ltd., manufacturing tests for varietal releaseM/s. VST Industries Ltd. andIndian Tobacco Association,Guntur

8 Uttar Banga Krishi Vishwa- Collaborative research programmesvidyalaya, Pundibari,Cooch Behar(W.B.)

9 TNAU, Coimbatore PG Studies, Research and Development

10 Gujarat Agricultural University Research and development

11 Acharya NG Ranga Agrl. University Research and development

12 UAS, Dharwad Research and development

organisations like Tobacco Board andDepartment of Biotechnology are associatedwith different tobacco developmentprogrammes. CTRI has also developed linkageswith ICAR organistions like NBPGR, New Delhi,CIAE, Bhopal and PDPC, Bangalore, IICT,Hyderabad, NIN, Hyderabad, NRC Grapes,Pune, Directorate of Groundnut Research,Junagadh.

71 ICAR - CTRI Annual Report 2015-16

13 Chandra Shekhar Azad University Research and developmentof Agriculture and Technology,Kanpur

14 Odisha University of Agriculture Research and developmentand Technology

15 University of Agriculture and Research and developmentHorticultural Sciences, Shimoga

16 Nannaya University, Rajahmundry PG Studies, Research

17 Andhra Univ., Visakhapatnam PG Studies, Research

18 NIFTEM, New Delhi Training of UG & PG students

19 Rallis India Limited Contract Research

20 ICAR- National Bureau of Coordinated trials in Biological controlAgricultural Insect Resources,Bangalore

21 Indian Institute of Spices Network Project on “Phytochemicals and HighResearch, Kokhikode, Kerala value compounds”

22 CSIR- Indian Institute of Collaborative studies on Characterisation andChemical Technology, Hyderabad refining of crude tobacco seed oil

23 National Institute of Nutrition, Pre-clinical efficacy and safety evaluation ofHyderabad Refined tobacco seed oil

(b) International Institutions

1 ISO-TC126, Berlin, Germany Development of international standards fortobacco and tobacco products

Sl. Name of the Collaborating Agency Project title/ActivityNo.

Linkages and Collaborations

ICAR - CTRI Annual Report 2015-16 72

All India Network Project on Tobacco

Table 2: Advanced Varietal Trials

Centre Promising line(s)

FCV tobacco

Guntur FCR-17, FCR-18, FCR-10 andFCR-11

Kandukur FCR-15 and FCR-10

Jeelugumilli FCJ-11, FCJ-13, FCJ-15, FCJ-5, FCJ-6 and FCJ-7

Rajahmundry FCR-12, FCR-17 and FCR-22

Bidi tobacco

Anand ABD 129, ABD 138, ABD 163,ABD 164, ABD 167, ABD 168andABD 169

Nandyal ABD 132, ABD 131 and NBD 260

Rustica tobacco

Anand LR 77, AR 111 , AR 118 and AR121

Araul ArR 29 and ArR-38

Ladol LR 77

Table 3: Bulk Evaluation Trials

Centre Promising line(s)

FCV tobacco

Rajahmundry FCR-12

Jeelugumilli FCJ-5 and FCJ-6

Bidi tobacco

Nandyal ABD 124 and ABD 119

Araul ArBD-09

Recommendations to tobacco farmers

● Results of a three years study indicated thatfor the purpose of oil and nicotine yield,variety A 145 and GABT 11 are most suitablewith higher dose of nitrogen (300 kg N/ha).

● The farmers of the middle Gujarat areadvised to apply emamectin benzoate 5 WG@ 0.0025% (5g / 10 l water; 7.5 g a.i./ha)for effective and economic control of leaf

Salient achievements from experimentsconducted at different AINPT centres during2014-15 are summarized as follows:

Varieties Released / identified

❖ Three FCV tobacco varieties viz., N-98 (forNLS), CH-1 (CMS hybrid for NLS), LT Kanchan(low tar for NLS) and one bidi variety,Nandyal Pogaku-1 were released forcommercial cultivation by the State SeedSub-committee on Agricultural/Horticultural Crops for the State of AndhraPradesh.

Coordinated Varietal Trials

The most promising lines identified in co-ordinated varietal trials conducted at differentcentres were as follows:

Table 1: Initial Varietal Trials

Centre Promising line(s)

FCV tobacco

Guntur FCR-24, FCR-26, FCJ-17 andFCJ-18

Kandukur FCR-29, FCR-31 & FCR-32

Jeelugumilli FCJ-16, FCJ-17, FCJ-18, FCJ-20, FCJ-21 and FCJ-22

Rajahmundry FCR-28, FCR-32, FCR-29, FCR-31, FCR-30 and FCR-26

Shivamogga FCR-29, FCR-32 and FCR-30

Bidi tobacco

Anand ABD 152 and NBD 261

Nipani ABD 131, NBD 257 , NBD 277 ,NBD 276 and ABD 152

Nandyal ABD 146, NyBD 56 and NBD 276

Rustica tobacco

Araul ArR-45 and LR-80

Ladol LR 78 and AR 126

Dinhata AR-126, ArR-45, ArR-46, LR-78,LR-79 and LR-80

73 ICAR - CTRI Annual Report 2015-16

All India Network Project on Tobacco

eating caterpillar infesting bidi tobacconursery.

● Bidi tobacco growing farmers are advisedto raise nursery during I to II week of Julyfor realising high number of healthyseedlings with higher net returns.

● Foliar application of nitrogen and potassium@ 2.5% in two splits markedly enhanced theproduction of top grade equivalent up to17-19% in KLS

● Minimum disease severity of brown spot(21.48 PDI) was recorded with two spraysof Hexaconazole (0.1%) and Difenconazole(0.1%) or Hexaconazole (5%) + Capton (0.2%)respectively at Nipani area in Karnataka.

● Azoxystrobin 23 SC (0.1%) followed byfenamidon + mancozeb (0.3%) isrecommended for controlling damping offdisease and higher number of healthyseedlings in the nursery.

● A new efficient system of heat conveyanceinside the curing chamber developed atShivamogga centre was found to save woodto the extent of 45% over the conventionalsystem.

Centre-wise research achievements

ANAND

❖ Application of 180 kg N/ha required forrealising optimum cured leaf yield in biditobacco at Anand, Gujarat.

❖ Application of 200 kg N/ha is required forrealising optimum cured leaf yield in rulingrustica tobacco.

❖ Genotype ASO 13 recorded higher seed andoil yield as compared to variety A 145. Thekhakhari yield, nicotine content andnicotine yield potential as well as oilcontent of seed were high in genotype 103-9-101. Variety GABT 11 showed highestgreen leaf biomass while protein contentwas highest in line 22-9-4-6.

❖ Various natural enemies like spider,coccinellids, Nesidiocoris tenuis, andRhinocoris sp were active and found ondifferent crops raised under entomophageBiodiversity Park in tobacco based agroecosystem. Predatory bug N. tenuis have

negative significant correlation with MIN Tand Mean T while, RH 2 showed positivesignificant correlation. Rhinocoris spp. havenegative significant correlation with RH 2.

❖ Spray of emamectin benzoate significantlyreduced seedlings damage by Spodopteralitura and recorded highest number oftransplantable seedlings in bidi tobacco.

❖ At Anand, significantly higher number oftransplantable and total seedlings wererecorded in III week of July sowings ascompared to those in I and III week ofAugust. Root-knot index was reduced withdelay in sowing.

ARAUL

❖ Entry ArBD-39 and ArBD-40 showedsignificant superiority in yield over thecheck A-119, and these entries are proposedfor testing in IVT during Kharif 2015-16.Entry ArBD-25 proved significant superiorityin yield over the check A-119 in AVT-I.

BERHAMPUR

❖❖❖❖❖ Among nine entries tested during kharif2014, the entry ‘NF-3-1’ produced 15.35%higher cured leaf yield (1407 kg/ha) overcheck variety ‘Gajapati’ (1222 kg/ha).

❖❖❖❖❖ Brinjal, chilli, tomato and cabbage withtobacco cured leaf equivalent yield of18.62, 17.67, 17.11, 16.75 q/ha respectivelywere at a par with tobacco (17.01 q/ha).But the highest net return of Rs 56,567/hawith B:C ratio 2.52 was achieved withtobacco. Hence, now no other crop is asprofitable as pikka tobacco.

HUNSUR

❖ Integrated farming system developed at thestation has been demonstrated to thefarmers, state department anddevelopment officials for its sustainability

❖ The Coconut husks/fronds in combinationwith fuel wood could be effectively utilizedas alternative source for curing.

❖ Tray nursery technology was furtherpopularise in the KLS area, the technologybeing adopted by more than 75-80 %growers.

ICAR - CTRI Annual Report 2015-16 74

❖ Green tech nursery using poly house forraising tobacco seedlings was demonstratedto farmers.

JEELUGUMILLI

❖ Lines Tobios-6 and NLST-4 performed betterin on-farm trials conducted in northern lightsoils of Andhra Pradesh.

NANDYAL

❖ Cured leaf yield was significantly superior(2266 kg/ha) with ridge planting comparedto traditional method of planting (1840 kg/ha). Among four spacing’s tried 60 cm x 75cm spacing recorded significantly highestcured leaf yield (2211 kg/ha).

❖ Cured leaf yield was significantly superior(2320 kg/ha) with NBD -119. Among N dosessignificantly higher cured leaf (2123 kg/ha)was recorded with 150 kg N/ha treatmentwhich is on par with 110 kg/ha (2098 kg/ha). Topping at 15 leaf recorded highestcured leaf yield (2243 kg/ha) which is onpar with topping at 18 leaf (2214 kg/ha).

❖ Foliar nutrition of N and K with KNO3 andAmmonium sulphate + SOP at 45 and 60 DATrecorded 11% increase yield in cured leafof Bidi tobacco.

NIPANI

❖ Topping at 18 leaves was found to besuitable for realizing maximum cured leafyield with bidi tobacco variety, NBD 209.

SHIVAMOGGA

❖ Genotype, NLST-2 x KST-28 II wassignificantly superior for all the threecharacters over the high yielding check,Thrupthi.

❖ Application of K @100 kg/ha showed highercured leaf yield (820 kg/ha) over 80 kg/ha.The yield and quality of the leaves tobaccostem ash (100 kg/ha K) was on par with thesuperior.

❖ Among the fifteen lines studied for rootingpattern, physiological and biochemicalparameters, Tobios-6 recorded moderateroot volume, root length, with lowestmembrane damage, followed by F-210, FCR-15, FCJ-13, Kanchan and Thrupthi. Lines,FCR-18, FCR-1 and Thrupthi showed highchlorophyll stability. The lines, FCR-16 andFCR-1 recorded higher biomass followed byKanchan Tobios-6 & FCR-2 .

❖ IVT entries FCR-23 and FCR-15 recordedhigher pollen and seed germination followedby FCJ-14, FCJ-12 and FCR-17 under highPEG concentration which indicates theirdrought tolerance capacity.

❖ IVT Lines, FCR-26 and FCJ-16 were foundresistant/ tolerant to black shank under sickplot condition.

❖ Among five tested combi-fungicidesevaluated sectin @ 0.3 % and Amistar @ 0.1% were equally found effective to as thatof recommended check ridomil gold (0.2 %)in reducing the damping-off disease innursery.

❖ Among the 25 germplasm lines screenedagainst root-knot nematode, ten entriesviz., FCR-18, FCR-19, FCK-4, FCJ-14, FCR-20, FCJ-11, Kanchan, FCR-23, FCJ-13 andFCR-21 recorded least root-knot index (1.0)indicating resistant reaction.

❖ Lowest RKI was observed with consortia ofbioagents and combination of Pseudomonasfluorescens and Acacia.

VEDASANDUR

❖ Drip fertigation recorded significantlyhigher FGLY, TCLY and WUE over the furrowirrigation or alternate furrow irrigation. TheWUE of drip fertigation and drip irrigationis comparable.

❖ Planting chewing tobacco hybrid VDH-3during the 1st fortnight of October with dripfertigation at 100% RDN increased the yieldand net returns.

All India Network Project on Tobacco

75 ICAR - CTRI Annual Report 2015-16

Empowerment of Women in Agriculture

The following programmes have been takenup for the benefit of empowering tribal farmwomen in the tribal area of East GodavariDistrict.

1. Health and Nutrition Education for Tribalwomen

About ten training programmes on healthand nutrition education, safe drinking waterprogramme, fruits and vegetable preservationmethods, post harvest technology wereconducted in the adopted villages viz.,Devarapalli, Sunnam Padu, Kutravada,Musurumelli, Ramanna valasa I.Pola varam &Seetha Palli, for the benefit of tribal womenduring the year. The backyard kitchen gardeningcomponent (greens, vegetables, orchards), thesupplementary diets with locally available foodsand nutrition education have played a key rolein reducing anaemia and malnutrition of womenand children to an extent of 60% in the adoptedtribal villages.

families and reduced the socio-economicproblems in the tribal area.

Training on value added food products

2. Addressing the Gender Specific Issues inTribal Area

The tribal women were exposed towardsmultiple skills and capacity buildingprogrammes for improving their knowledge,skills, and abilities. About five training programswere conducted on up-gradation of vocationalskills, technical & market tie up, management& decision making skills and individualcounseling for increasing their self confidence,self-esteem and self efficacy. This has enrichedthe farm and home environment of tribal farm

Capacity building programme at Seethapallitribal village

3. Drudgery reduction and improving theefficiency of farm labour

Drudgery reducing farm implements wereintroduced and popularized in order to improvethe farm efficiency of tribal women. Improvedagricultural implements viz., wind mill (paddythreshing), wheel hand- hoe (vegetables),improved krishi sprayers, leaf plate makingmachinery were popularized in Maredumilli,Sunnampadu, I.Polavaram and Pedageddadavillages for saving time and drudgery and forimprovement of farm efficiency among tribalwomen. After introduction of this simplemachinery, the drudgery was reduced andefficiency of the farm work was improved.About six programmes were conducted onoccupational health and drudgery deductionduring the year.

Drudgery saving farm implements at Devarapalli

ICAR - CTRI Annual Report 2015-16 76

Empowerment of Women in Agriculture

4. Crop Diversification of Farming Systems:

Subsidiary income generating programmesviz., kitchen gardening, poultry keeping, fodderproduction, grafting techniques and valueaddition to the minor forest produce weredifferent training components added to tribalfarm families to enhance the farming systemduring lean period which enhanced the familyincome in tribal area.

Thus, a total of 16 training programmes,12 demonstrations, 2 exposure visits, onenutrition work shop and 5 long durationcapacity building programmes, oneinternational women’s day, one women inagriculture day were conducted for the benefitof 500 tribal families of East Godavari Districtfor brining awareness, improving knowledge andskills and for integrating the tribal women inthe national mainstream.

Skill Development Programme on FoodProcessing:

KVK in association with NITEM (NationalInstitute of Food Technology Entrepreneurshipand Management), New Delhi organized SkillDevelopment Programme on Food Processingduring 22-26 September 2015 at KVKKalavacharla. A total no of 52 rural women ofdifferent Self Help Groups from Kalavacharla,Gadarada, Narasapuram villages haveparticipated in this programme.

Turkey bird rearing at Geddada

5. Homestead Units and EntrepreneurialActivities:

Nutri-preneurial (diversified agricultural)homestead units viz., solar drying, soyaprocessing, millet based bakery units, leaf platemaking and value addition(Minor ForestProduce) units were established in tribal villagesviz., Devarapalli, Irlapalli, Seethapalli,Kutravada, Ramanna Gudem and Sunnampadu.This has enhanced the annual family income ofthe tribal women up to an extent of Rs. 10,000.The women were also provided technical andmarketing assistance along with skill up-gradation.

Homestead Units in Devarapalli supported byICAR-CTRI

● Vocational training programme on‘Garment making’ was conducted during 6July-5 August 2015 at Narsapuram villageof Korukonda mandal (E.G dist) for 30 ruralwomen in association with World VisionNGO.

● Training programme on ‘Value addition tomillet” was conducted during 22-25September 2015 at KVK, Kalavacharla for20 rural women.

● Training programme on ‘Milky mushroomproduction” was conducted during 26-29September 2015 at Vemagiri village for 10rural women.

● Training programme on ‘Value addition to

77 ICAR - CTRI Annual Report 2015-16

Fruits and Vegetables” was conductedduring 4-7 October 2015 at Pasarlapudilankavillage of P. Gannavaram mandal) for 20rural women.

● Awareness programme was conducted on 6October 2015 on ‘Artificial ripening of fruitsand its ill effects’ at Mukkamala village ofAmbajipet mandal for 20 rural women and28 girl students of final year B. Sc (Hort.),YSRHU.

● Training programme on ‘Value addedproducts with coir’ was conducted during3-19 November 2015 at Babanagar villageof Tallarevu mandal (E.G dist) for 15 fisherwomen in association with EGREEFoundation-NGO.

● Training programme on ‘Palmyrah fibreextraction’ was conducted during 24-29th

November 2015 at Sirsinapalli andTulasipaka villages of Chinturu mandal (E.Gdist) for 10 tribal women in association withASHA Foundation-NGO.

● Training programme on ‘Friends of CoconutTree (FoCT)’ was conductedduring 20-25th

January 2016 at KVK Kalavacharla, for 20rural youth/farmers in association CoconutDevelopment Board, Cochin and HRS,Ambajipet.

● Training programme on ‘Protray vegetableseedling production’ was conducted during3-6th February 2016 at Mirthipadu village,Korukonda mandal.

● Training programme on ‘Handicrafts withPalmyrah leaves’ was conducted during 5-15 October 2015 at KVK, Kalavacharla for10 rural women.

● Training programme on ‘Coirpithcomposting’ was conducted during 5-15October 2015 at KVK, Kalavacharla for 10rural women.

● Vocational training programme on ‘MaggamEmbrodery and Garment making’ wasconducted during 1st Oct – 30th Nov 2015 atPalasarla village of Rajanagarm mandal (E.Gdist) for 20 rural women.

● Training programme on ‘Value addedproducts with milk’ was conducted during3-7th November 2015 at Chollangipetavillage of Tallarevu mandal (E.G. dist) for15 fisher women in association with EGREEFoundation-NGO.

● Training programme on ‘Value addition tofish/prawn and meat’ was conducted during12-15th February 2016 at Ainavilli of Ainavillimandal for 20 rural women.

● Training programme on ‘Handicrafts withWeeds’ was conducted during 14-24th March2016 at KVK, Kalavacharla.

Empowerment of Women in Agriculture

ICAR - CTRI Annual Report 2015-16 78

List of publications

Anuradha, M., T.G.K. Murthy, D. Damodar Reddyand D.V.L. Satyavathi 2015. Variability innet photosynthetic rate, water useefficiency and carboxylation efficiencyof tobacco varieties. Tob. Res. 41(1): 43-48.

Chandrasekhara Rao, C., B. Krishna Rao, M.Anuradha and H. Ravisankar. 2015. Effectof drip fertigation on yield, quality andeconomics of flue-cured Virginia tobacco (N.tabacum) grown in irrigated Alfisols ofAndhra Pradesh. Tob. Res. 41(1): 37-42.

China Chenchaiah K. 2015. Efficacy of plantextracts and inorganic salts againstcigarette beetle Lasioderma serricorne (f).Tob. Res. 41(2): 105-108.

Ghosh, R.K., Damodar Reddy, D. and Deb PrasadRay. 2016. “Removal of Cationic dye,methylene blue from water by Cotton Stemsash as a novel absorbent”. InternationalJournal of Agriculture, Environment andBiotechnology, 9(2): 237-245.

Ghosh, R.K., and Damodar Reddy, D. 2015Biomarkers: A tool for monitoring pesticidepollution. International Journal ofBioresource Science, 2(2): 111-128.

Ghosh, R.K., Deb Prasad Ray, SomsubhraChakraborty, Kaushik Majumdar andDamodar Reddy D. (2015). Pesticides inenvironment and their managementstrategies. International Journal ofBioresource Science, 2(11): 47-54.

Gunneswara Rao S. and U. Sreedhar. 2015.Determination of economic injury level forthe tobacco capsule borer, Helicoverpaarmigera (Hubner) on FCV tobacco. Tob.Res. 41(1):1-5.

Mallikarjuna Rao, K.L.M., K. Siva Raju and H.Ravisankar. 2015. Biochemical andmolecular characterization of nativeisolates of Trichoderma antagonistic againstSclerotium and Pythium infecting tobacco.Int.J. Curr. Microbiol. Appl. Sci. 4 (7): 977-989

Mallikarjuna Rao, K.L.N., K. Siva Raju and H.Ravisankar. 2015. Antifungal properties of

native Trichoderma isolates againstSclerotium rolfsii and Pythiumalphanidermatum infecting tobacco. J.Environ. Biol. 36: 1349-53.

Naidu V.S.G.R. 2015. Climate change, crop-weedbalance and the future of weedmanagement. Indian J.l Weed Sci. 47(3):288–295.

Naidu, V.S.G.R., Gulshan Mahajan, SubhashChander and Bhumesh Kumar. 2015. Crop-Weed Competition and Management underChanging Climate. Indian Farming 65 (7).Pp: 40-43.

Naidu, V.S.G.R., H. Ravisankar, Sandeep Dhagat,VirendraKamalvanshi and A.R. Sharma.2015. Expert system for weed seedidentification. Indian J.l Weed Sci. 47(2):197-200.

Poorna Bindu, J., D. Damodar Reddy, P. Santhy,K.M. Sellamuthu, M. Mohammed Yassin andRavindra Naik. 2015. Production andcharacterization of tobacco stalk biochar.Tob. Res. 41(2): 91-96.

Raghupathi Rao, G., U. Sreedhar and S.K. Dam.2015. Influence of insecticide formulationsand discharge rate on spray characteristicsand incidence of major insect pests on FCVtobacco. Tob. Res. 41(2): 62-65.

Ramakrishnan, S., K.N. Subrahmanya, T.G.K.Murthy and K. Sarala. 2015. Varied reactionsof FCV tobacco germplasm against root-knotnematodes, Meloidogyne sp. Tob. Res.41(1): 49-50.

Ravisankar, H., Y. Subbaiah and T.G.K. Murthy.2015. Decision Support System for transferof technology on FCV tobacco production.Int. J. Appl. Res. Information Technol.Computing 6(2) : 94-9.

Ravisankar, H., K. Sivaraju and D. Damodarreddy.2016. Agricultural Expert Systems: Currentstatus and future challenges: In: Souvenirof 8th GCRA International conference onInnovative digital applications forsustainable development. Pp:

Satyanarayana, K.V., K. Siva Raju, V.U.M. Sarmaand C.V. Narasimha Rao. 2015. Lipophilic

79 ICAR - CTRI Annual Report 2015-16

List of Publication

constituents in HDBRG tobacco. Tob. Res.41(2): 77-86.

Sheshu Madhav M., P. Rajendra Kumar, K. SivaRaju, B. Vishalakshi and S.J.S. Rama Devi.2015. Phytogenetic reconstruction of fivesolanaceous species by genome-wideanalysis of simple sequence repeats inorganellar genomes and their utility inestablishing species relationships of genusNicotiana. Curr. Trends Biotech. Pharm.9(2): 107-116.

Siva Raju K., D. Damodar Reddy, C V NarasimhaRao. 2015. Comparative study oncharacteristics of seed oil and nutritionalcomposition of seed cake in differenttobacco types cultivated in India. Tob. Res.41(1): 6-14.

Siva Raju, K., D. Damodar Reddy and T.G.K.Murthy. 2015. Effect of genotype and leafposition on pigments and their degradationderivatives in burley tobacco. Tob. Res.41(2): 66-71.

Siva Raju, K., T.G.K Murthy and H. Ravisankar.2015. SSR based genetic diversity in Indianflue-cured Virginia (Nicotiana tabacum)cultivars. Int. J. Plant Biochem. Biotech.11(2): 149-159.

Siva Raju. K., D. Damodar Reddy, C.Chandrasekhara Rao and Y. Subbaiah. 2015.Effect of nitrogen and leaf position onquality constituents of Lanka tobacco(Nicotiana tabacum, L.) grown in AndhraPradesh. Tob. Res. 41(2): 55-61.

Srihari, C.V.N., K. Siva Raju and C.V. NarasimhaRao. 2015. Neutral volatile compounds inOriental tobacco grown in India. Tob. Res.41(2): 97-104.

Subbaiah, Y. and D. Damodar Reddy. 2015.Smallholder family farming of FCV tobaccoin Karnataka – A socioeconomic evaluation.Tob. Res. 41(2): 72-6.

Subhashini, D. V. 2015. Population density andin vitro characterization of selected PGPRsfrom tobacco rhizosphere soils. J. Biol.Contl 29(4): 207-212.

Subhashini, D.V and T G K Murthy. 2015.Genotype dependant variation in arbuscularmycorrhizal colonization of tobacco(Nicotiana tabacum L.) Ind. J. Agric Sci.85(8): 1118-22.

Suman Kalyani, K. and T.G.K. Murthy. 2015.Women Empowerment through Soya MilkProduction and Consumption in Tribal Areasof East Godavari District, Andhra Pradesh.Indian Res. J. Ext. Edu. 15 (4): 65-69.

Suman Kalyani, K., V. Krishnamurthy and C.Chandra Sekhara Rao. 2015. Farmingsystems for the tribal area of East GodavariDistrict of Andhra Pradesh. Indian Farming.65(8): 17-20.

Sunil Mandi, S. Kasturi Krishna, D. DamodarReddy and S.V. Krishna Reddy. 2015. Weedseeds stratification in contrasting soiltexture on the bank of river Godavari. Int.J. Agricul. Environ. Biotech. 8(3): 669-73.

Upadhyaya, A., Singh, S., Prasad, L.K., and Roy,M. 2015. Spatial and temporal variation ofsoil moisture under different tillagepractices in wheat Crop. J. Agri. Search,2(3), 175-8.

Leaflets/Folders/ Technical Books/Bulletins:

● FCV Tobacco production technology andGood Agricultural Practices in KLS (inEnglish).

● Soil health management in FCV tobaccogrowing soils of KLS (Technical folder)

● V.S.G.R. Naidu and E. Vijaya Prasad. 2015.Zero Tillage Maize cultivation.

● V.S.G.R. Naidu and J.V.R.Satyavani. 2015)Toxic effects of artificial ripening of fruits.

● V.S.G.R. Naidu and J.V.R.Satyavani. (2015Value added products with cashew apple.

● V.S.G.R. Naidu and R. Sudhakar. 2015.Coconut Coir pith compost making,

● V.S.G.R. Naidu and V. V. Lakshmi Kumari.2015. Value added products with cocoa.

● V.S.G.R. Naidu, B. John Babu, P. V. V. S. SivaRao. 2015) Rearing of Black Bengal goats.

ICAR - CTRI Annual Report 2015-16 80

List of Approved On-going Projects

Sl. Institute Title of the project and Investigator(s)No Code

CROP IMPROVEMENT

1 Br.2. Evolving superior varieties of FCV tobacco through hybridizationT G K Murthy, P V Venugopala Rao, K. Sarala, A V S R Swamy andK. Prabhakara Rao

2 Biotech-6 Molecular Mapping of Important Tobacco TraitsK. Sarala and K. Prabhakara Rao

3 Biotech-11 Biogenesis and regulation of TSNA (Tobacco Specific Nitrosamines) inTobaccoK. Prabhakara Rao and K. Sarala

CROP PRODUCTION

4 A-83 Chemical management of Orobanche in FCV tobaccoS. Kasturi Krishna, S.V. Krishna Reddy and V.S.G.R. Naidu

5 A-84 Studies on false maturity and its mitigation strategies in FCV tobacco growingregions of Andhra PradeshS.V. Krishna Reddy, M. Anuradha, S. Kasturi Krishna, P. Venkateswarlu andK. Cahnchaiah

6 Ag. Extn-50 Technology evaluation demonstration and impact analysisY. Subbaiah, and K.Suman Kalyani

7 ARIS-15 Tobacco Agridaksh: An online expert systemH. Ravisankar

8 OC-24 Studies on chemical constituents responsible for smoke flavour in FCV tobaccogrown under different agro climatic zonesK. Siva Raju, TGK Murthy and D. Damodar Reddy

9 SSMB-12 Tobacco (Nicotiana tabacum) leaf and stem assisted green synthesis of slivernanoparticles and evaluation of its antimicrobial activity against agriculturalplant pathogensD.V. Subhashini

10 SS-31 Evaluation of crop residue and wood ashes effects on soil fertility andpotassium nutrition of tobaccoD.damodar Reddy, S. Kasturi Krishna, M. M. Swamy, L.K. Prasad,K. Nageswara Rao and Jana Poorna Bindu

11 SS-32 Evaluation of organic and inorganic soil amendments to minimize nutrientleaching losses and enhance nutrient use efficiency under NLS tobaccoproduction systemJ. Poorna Bindu and D Domodar Reddy

81 ICAR - CTRI Annual Report 2015-16

Sl. Institute Title of the project and Investigator(s)No Code

12 SSK-2 Assessment of leaf quality of FCV tobacco using hyper-spectral radiometricremote sensing techniquesDr. L.K. Prasad, M. Anuradha, M. Prabhakar(CRIDA) and D. Damodar Reddy

CROP PROTECTION

13 E-81 Bio efficacy and field evaluation of new pesticides against tobacco insectpests and diseasesDr U Sreedhar, G. Raghupathi Rao, S. Ramakrishnan and S.K. Dam

CTRI RESEARCH STATION: JEELUGUMILLI

14 JL.Br.2.1 Evolving flue-cured tobacco varieties having high yield and better qualitysuitable for NLS area of Andhra PradeshA V S R SwamyT G K Murthy, K. Sarala and K. Prabhakara Rao

15 JLA-37 Effect of drip irrigation and tray seedlings on the productivity of NLStobacco.S. V. Krishna Reddy, C. C.S. Rao and S. Kasturi Krishna

16 E-82 Evaluation of insecticide application technology for the effective spraycoverage on FCV tobacco in NLSDr G. Raghupathi Rao, U. Sreedhar and K. Nageswara Rao

BTRC, KALAVACHERLA

17 AB-30 Set row planting in burley tobaccoC.C.S. Rao

CTRI RESEARCH STATION: GUNTUR

18 EG.14 Validation of IPM module against tobacco aphid, Myzusnicotianae under CBSconditionsP. Venkateswarlu

CTRI RESEARCH STATION: KANDUKURU

19 K.Br.6 Breeding FCV Tobacco varieties for yield and quality under Southern LightSoil (SLS) conditions P V Venugopala Rao

20 Phy.K-1 Abiotic stress management interventions for climate resilient flue curedtobacco production in SLS Domain of A.PM. Anuradha

21 EK-19 Evaluation of IPM modules for the management of caterpillar and aphid inFCV tobacco under SLS conditionsK.C. Chanchaiah

List of Approved On-going Projects

ICAR - CTRI Annual Report 2015-16 82

Sl. Institute Title of the project and Investigator(s)No Code

List of Approved On-going Projects

CTRI RESEARCH STATION: HUNSUR

22 Br.19 Development and evaluation of F1 hybrids of FCV tobacco suitable to Karnatakalight soil regionC. Nanda

23 N-20 Integrated management of root- knot nematodes in FCV tobaccoS. Ramakrishnan and P.Nagesh

24 A-41 Studies on climate Risk Management practices for FCV tobacco based croppingsystem in STZ of KarnatakaM. Mahadevaswamy and C. Chandrasekhara Rao

CTRI RESEARCH STATION: VEDASANDUR

25 B.50 Breeding non FCV tobacco types for desirable traitsA V S R Swamy and T G K Murthy

CTRI RESEARCH STATION: DINHATA

26 A-10 Permanent manurial experiment with Motihari tobaccoSunil Mandi

83 ICAR - CTRI Annual Report 2015-16

RAC, QRT, IRC and IMC Meetings

RESEARCH ADVISORY COMMITTEE

Dr. A. Padma Raju, ChairmanVice-Chancellor,ANGRAU, Hyderabad

Dr. V. S. Korikanthimath, MemberRetd. Director,ICAR Research Complex, Goa

Dr. J.L. Karihaloo, MemberCoordinator, APCoAb, C/o ICRISAT,NASC Complex, Pusa,New Delhi – 110 012.

Dr. Ashok Kumar, MemberProfessor and Head,Department of Soil Science,College of Agriculture,SVBPUA&T, Meerut

Dr. T.V.K. Singh, MemberDean of AgricultureProfessor Jayashankar Telangana StateAgricultural University (PJTSAU),Hyderabad

Director, MemberICAR-CTRI, Rajahmundry

ADG (CC), MemberICAR, New Delhi

IMC Member Member

Dr. K. Sarala, Member-Principal Scientist & Head Secretary, RACICAR-CTRI, Rajahmundry

The Research Advisory Committee (RAC) meeting of ICAR-CTRI was held on 2nd May, 2015 atICAR-CTRI, Rajahmundry under the Chairmanship of Dr. A. Padma Raju, Vice-Chancellor, AcharyaN.G.Ranga Agricultural University, Hyderabad. RAC members, Dr. V. S. Korikanthimath, Dr. T.V.K.Singh and Dr. Ashok Kumar attended the meeting. Dr. D. Damodar Reddy, Director, ICAR-CTRI, Dr.K. Sarala, Member-Secretary, RAC, Heads of Divisions, Heads of Research Stations, Scientists andHeads of administration of ICAR-CTRI participated in the meeting.The committee reviewed theresearch being conducted at CTRI & its Research Stations and advised few priority areas forinitiating the research.

ICAR - CTRI Annual Report 2015-16 84

Dr. R.R. Hanchinal, ChairmanChairperson,Protection of Plant Varieties and FarmersRight Authority,Govt. of India, Min. of Agriculture,Dept. of Agriculture and Co-operation,New Dehi -110 012.

Dr. B.N. Bhat, MemberEx- Associate Director of ResearchAgril. Research Station,Nipani-591237,Karnataka

Dr. R. Lakshminarayana, MemberRetd. Principal Scientist & Head andFormer Project Co-ordinator,Rajahmundry – 533105.

Dr. T. Ramesh Babu, MemberDean, PG Studies,ANGRAU, Rajendranagar,Hyderabad – 500 030

QUINQUENNIAL REVIEW TEAM

Dr. R.K. Samanta MemberFormer Vice-Chancellor,BCKV, WB and Former Director,NAARM and MANAGE, HyderabadHyderabad – 500 030.

Prof. Satyendra Chandra Sarker, MemberAgricultural Economics,Dept of Agricultural Economics,UBKV, Cooch Behar-736165West Bengal

Dr. C. Chandrasekhara Rao, Member-Head, Division of Crop Chemistry Secretaryand Soil ScienceCTRI, Rajahmundry,Andhra Pradesh

RAC, QRT, IRC and IMC Meetings

QRT team visited the CTRI RS, Dinhata on10.05.2015.,CTRI RS, Vedasandur on 16.05.2015CTRI, Rajahmundry during 28.11.2015 to 30.11.2015.The drat QRT report was prepared.

85 ICAR - CTRI Annual Report 2015-16

INSTITUTE MANAGEMENT COMMITTEEDr. D. Damodar Reddy ChairmanDirector,CTRI,Rajahmundry

Dr. K. Siva Raju MemberPr. ScientistCTRI,Rajahmundry

Dr. R.K. Mathur MemberPr. ScientistDOPR,Pedavagi

Dr. Y.G. Prasad MemberPr. ScientistCRIDA, Hyderabad

Dr. K. Anitha MemberPr. ScientistNBPGR Regional Station,Hyderabad

ADG (CC) MemberICAR, New Delhi

SAO Member-SecretaryCTRI, Rajahmundry

INSTITUTE RESEARCH COMMITTEE (IRC) MEETINGSThe Institute Research Committee

Meetings (IRC) were held during 24-26 August,2015 at ICAR-CTRI, Rajahmundry for reviewingthe research carried out during the year 2014-15 and finalizing technical programme for 2015-16. Dr. D. Damodar Reddy, Director, ICAR-CTRI,Rajahmundry, Dr. K. Gopal, IAS, Chairman,

Tobacco Board, Guntur, Dr. K. Deo Singh, FormerDirector, ICAR-CTRI, trade representatives,Tobacco Board officials, farmers and scientistsparticipated in the meetings. In this connection,a Scientist-Farmer-Trade-Board Interface wasorganized for the identification andprioritisations of researchable issues.

48thInstitute Management Committee meeting was held on 09.06.2015 at ICAR-CTRI,Rajahmundry and discussed about various management issues related to the institute.

49th Institute Management Committee meeting was held on 20.11.2015 at ICAR-CTRI,Rajahmundry and discussed about various management issues related to the institute.

RAC, QRT, IRC and IMC Meetings

ICAR - CTRI Annual Report 2015-16 86

Participation of Scientists in Conferences,Meetings, Workshops and Symposia

Sl. Participant (s) Programme attended Date and placeNo.

1. Dr. M. Kumaresan, 22nd Scientific Advisory Committee 11.04.2015 atmeetings at KVK, Gandhigram Rural Gandhigram RuralUniversity University, Dindigul

2. U. Sreedhar 4th Congress on Insect Science 16-17th April, 2015 at(CIS-4) – Entomology for Sustainable Punjab AgriculturalAgriculture University, Ludhiana.

3. Dr. D. Damodar Reddy 32nd meeting of ISO/TC 126 and its 20-22 April, 2015 inworking bodies as part of Indian Zurich, Switzerland.delegation in the Capacity ofChairman, FAD-4 of Bureau ofIndian Standards

4. Dr P. Venkateswarlu Member, DPC, for promotion of 7th May, 2015 atManagers of Tobacco Board, Guntur Tobacco Board, Guntur

5. Dr.VSGR Naidu Annual Action Plan and Review 11-12 May, 2015 atmeetings of KVKs Dr.YRS Horticultural

University,Venkataramannagudem

6. Dr.VSGR Naidu, State-level Technical Programme 12-14 May, 2015 atDr.B.Johnbabu, (SLTP) of KVKs: Agricultural College,

Tirupathi

7. Dr P. Venkateswarlu Biological Workshop 2-3 June, 2015 atTNAU, Coimbatore

8. Dr. K. Siva Raju Meeting of FAD 4 11-13 June, 2015 atBIS, New Delhi

9. VSGR Naidu Annual Zonal workshop of KVKs of 26-28 June 2015 atZone-V Jalgaon, Maharashtra

10. Dr. T.G.K. Murthy Meeting of AP State Seed Sub 29th June2015 atDr. K. Sarala Committee for Varietal Release HyderabadDr. K. Prabhakara Rao

11. Sri Sunil Mandi Advisory Committee meeting 2nd July, 2015organized by KVK under UBKV,Pundibari.

12. Dr P. Venkateswarlu Member, Review Committee, 3rd July, 2015 atTobacco Board, Guntur Tobacco Board, Guntur

87 ICAR - CTRI Annual Report 2015-16

Sl. Participant (s) Programme attended Date and placeNo.

13. Dr. T.G.K. Murthy Meeting of AP State Seed Sub 10th July, 2015 atDr. K. Sarala Committee for Varietal Release HyderabadDr. K. Prabhakara Rao

14. Sri Sunil Mandi Advisory committee meeting 22nd July, 2015organized ACMART (FarmersCooperative Training Centre) a jointbody by NABARD and West BengalState Cooperative Ltd.

15. Dr. L.K. Prasad 9th National Conference of KVKs 25-26 July, 2015 atDr. V.S.G.R. Naidu Patna, Bihar

16. Dr. T.G.K. Murthy Meeting of AP State Seed Sub 03rd August, 2015 atDr. K. Sarala Committee for Varietal Release HyderabadDr. K. Prabhakara Rao

17. Dr. H. Ravisankar First Workshop of Nodal Officers of 4-5th August, 2015 atICAR Research Data Repository for NASC Complex,Knowledge Management New Delhi

18. Sri Sunil Mandi Interaction meeting with ICAR 22nd August, 2015 atGoverning Body member NIRJAFT, Kolkata

19. Dr. Y. Subbaiah Interviewing Committee for filling BCT-KVK to be held onup the posts of Programme 22nd August, 2015 atCoordinator and Subject Matter Haripuram.Specialist (Home Science) andProgramme Assistant (Veterinary)

20. Dr. K. Siva Raju Review meeting of Phytochemical 7th September, 2015 atproject IISR, Kozhikode

21. Sri Sunil Mandi Midterm Review Committee 19-20 Sept., 2015 atmeeting of ICAR Regional CIFRI, BarrakpurCommittee – II

22. Dr P. Venkateswarlu Member, ACP for promotions of 28th September, 2015 atAuction Superintendents, SGOs & Tobacco Board, GunturField Assistants and Member MACP

23. Scientists, CTRI AINPT Workshop 30 Sep -1st October,2015 at CTRI ResearchStation, Hunsur

24. Dr. S. Kasturi Krishna Silver Jubilee conference of 13-16 October, 2015 atDr. V.S.G.R. Naidu Asian-Pacific Weed Science Society PJTSAU, Hyderabad

(APWSS)

Participation of Scientists in Conferences, Meetings, Workshops and Symposia

ICAR - CTRI Annual Report 2015-16 88

Sl. Participant (s) Programme attended Date and placeNo.

25. Sri Sunil Mandi Advisory committee meeting 15th October, 2015organized ACMART (FarmersCooperative Training Centre ) a jointbody by NABARD and West BengalState Cooperative Ltd.

26. Dr. S. Kasturi Krishna Workshop on developing a road map 21st October, 2015 atfor Technological support to farmers Indian Institute ofin Southern Plateau & Hill Region Oilseed Research(Agro-climatic zone-X) (IIOR), Hyderabad

27. Dr. K. Suman Kalyani 11th International Food Data 3-5 November, 2015 atConference NIN, Hyderabad

28. Ms. J. Poorna Bindu 80th Annual Convention of ISSS and 5-8 December, 2015 atNational Seminar on “Developments GKVK, UAS, Bangalorein Soil Science-2015”

29. Dr. D.V. Subhashini 56thAnnual Conference of Association 7-10 December, 2015 atof Microbiologists of India (AMI-2015) Jawaharlal Nehru& International Symposium on University, New Delhi“Emerging discoveries inMicrobiology”

30. Dr. M. Anuradha 3rd International Plant Physiology 11-14 December, 2015Dr. K. Siva Raju Conference on “Challenges and at Jawaharlal Nehru

Strategies in Plant Biology Research” University, New Delhi

31. Dr. H. Ravisankar 8th CGRA International Conference 5-7 January, 2016 aton Innovative Digital Applications for GKVK, BangaloreSustainable Development

32. All Scientists of CTRI National Seminar on “Crop 18th January, 2015 atDiversification” CTRI, Rajahmundry

33. Dr. U. Sreedhar Conference on National Priorities in 4-5 February, 2016 atDr. G.Raghupathi Rao Plant Health Management S.V. Agricultural

College, Tirupati

34. Dr. U. Sreedhar Indian Ecological Society 18-20 Feb., 2015 atInternational Conference – Natural SKUAST, JammuResources Management EcologicalPerspectives

35. Dr. S.K. Dam National Symposium on Microbial 18-19 February, 2016 atDiversity and its impacts Kolkata, West Bengaland their management”

Participation of Scientists in Conferences, Meetings, Workshops and Symposia

89 ICAR - CTRI Annual Report 2015-16

Sl. Participant (s) Programme attended Date and placeNo.36. Dr AVSR Swamy Attended Eastern Zone Regional 19 - 21 February, 2015

Agricultural Fair at CPR Station Patna,Bihar

37. Dr. K. Siva Raju Meeting of FAD 4 7-9 March, 2015 at BIS,New Delhi

38. Sri Sunil Mandi Advisory committee meeting 15th March, 2016organized ACMART ( Farmers’Cooperative Training Centre ) a jointbody by NABARD and West BengalState Cooperative Ltd.

Participation of Scientists in Conferences, Meetings, Workshops and Symposia

ICAR - CTRI Annual Report 2015-16 90

Sl. Participant (s) Programme attended Date and placeNo.

1. Dr. C. Chandrasekhara Workshop on Right to Information 27-28 April, 2015 atRao Act, 2005 ISTM, New Delhi

3. Dr. K. Siva Raju Training programme for Technical 20-21 July, 2015 atCommittee members, BIS on the NITS, Noidaconcept and process of formulatingstandards to increase theirawareness on standard formulationwork

4. K. Padmaja 7th Capacity Building Programme for 28.09.2015 toJ. Vasanthi Technical Personnel 09.10.2015 at IIPA,

New Delhi

5. V.S.R.C. Murthy Training on Additional knowledge 5-7 October, 2015 atCh. Jayaram enhancement training on MIS/FMS IASRI, New Delhi

6. B. Krishna Kumari Training programme on “Agricultural 5-9 October, 2015 atKnowledge Communication” NAARM, Hyderabad

7. Dr. C. Nanda Winter School on “Advanced 8-28 October, 2015 atBreeding Strategies for Biotic and IIHR, BangaloreAbiotic Stress Tolerance in VegetableCrops”

8. P.V.S. Bharati Management Development 16-21 Nov., 2015 atprogramme on public procurement NIFM, Faridabad

9. E. Vijay Prasad Workshop cum training on pulses 22-23rd December,2015 at ATARI, Zone-V,Hyderabad

10. V. Bhagyalakshmi Workshop on communication skills 2 days5-6 Jan., 2015 atK.T.R. Singh ISTM, New Delhi

11. E. Vijay Prasad Training programme on IPM in Major 6-8th January, 2016 atcrops ATARI, Zone-V,

Hyderabad

12. Dr. K. Suman Kalyani Workshop on Competency 10-13 Feb., 2016 atDevelopment programme for NAARM, HyderabadNodal Officers of HRD

13. Dr. K. Siva Raju Refresher course on “Processing and 16-18 March, 2016 atanalytical methods of oils & fats” IICT, Hyderabad

Trainings and Capacity Building

91 ICAR - CTRI Annual Report 2015-16

Sl. Participant (s) Programme attended Date and placeNo.

14. T. Krishna Reddy Refresher cum skill upgradation 16-19 March, 2016 atK. Sesha Sai training on “Field crop management” ICAR-CTRI,I.Jagadish Chandra RajahmundryDr. S.K.DamB. Raja RaoM.TrinadhP.S.S.Prakasa RaoV.Narasimha MurthyM.M. Ali V.V.RamanaB.V.SrinivasCh.Sudhakara BabuD.S.R.SastryM.SrinivasK.V.RamanaS.Ramaraju

15. B.Nageswara Rao Refresher cum skill upgradation 16-19 March, 2016 atD.Balarama Reddy training on “Field crop & lab ICAR-CTRI,A.Nageswara Rao management” RajahmundryA.SrinivasG. Prasada RaoKVSS.Bhaskara RaoVVPL AcharyuluY.N.V.V.S.N.MurtyB. Koteswara RaoCh. SatyanarayanaY. SubrahmanyamP.SatyavathiM.SrilathaK.V.Narasimha Raju K. SatyanarayanaB .S.S.SaiCh.Subba RaoEvani RadhakrishnaA. Daniel RajuP.SubbayammaY.Jayalakshmi

Trainings and Capacity Building

ICAR - CTRI Annual Report 2015-16 92

Workshops, Seminars and Farmers’ Daysorganised by the Institute

Tobacco Scientist-Farmer-Trade-BoardInterface

Tobacco Scientist-Farmer-Trade-BoardInterface was organized on 24.08.2015 at ICAR-CTRI. Chairman, Tobacco Board; Director andScientists from CTRI, tobacco farmers from NLS,SLS and representatives of trade & industryparticipated.

AINPT Tobacco Workshop

The Biennial XXII Tobacco Workshop of AllIndia Network Project on Tobacco was held atICAR-CTRI-RS, Hunsur during 30th September &1st October, 2015 to discuss the research resultsof 2014-15 and finalization of TechnicalProgramme for 2015-16. The Workshop wasinaugurated by the Chief Guest Shri Bipin BihariChowdary, Executive Director (FAC) & Director(Auctions), Tobacco Board, Bangalore. Dr. T.G.K.Murthy, Nodal Officer, AINPT presented thestatus of research work and issues to bediscussed. Dr. D. Damodar Reddy, Director, ICAR-CTRI & Co-ordinator, AINPT addressed thegathering and presented the importance oftobacco in Indian economy along with AINPTsalient research achievements.

AP- TEC 2015

Andhra Pradesh Technology Developmentand Promotion Centre (APTDC), Hyderabadorganised an exhibition in technicalcollaboration with ICAR-CTRI from 05.06.2015to 07.06.2015. About 10,000 progressive farmersof the district visited the stalls and gotbenefitted through this programme.

Mera Gaon Mera Gaurav

Mera Gaon Mera Gaurav (My Village MyPride) was initiated at ICAR-CTRI, Rajahmundryin the month of November, 2015. The scientistsof ICAR-CTRI were divided into seven groups viz.,Godavari Group,Gowthami Group, PolavaramGroup, Krishna Group, Pattiseema Group,Gundlakamma Group,Kaveri Group foridentification of villages in a cluster/ locality

as per the convenience. The scientists haveadopted villages and completed the base linesurvey of the village. The regular activities viz.,farmers’ advisory services were carried out fromtime to time.

Jai Kisan Jai Vigyan week

Jai Kisan Jai Vigyan week was organised byICAR-CTRI at Rajahmundry from 23-29December, 2015 for promoting the use of sciencefor the welfare of farmers. The week wasinaugurated by the Chief Guest, Mr. ChiranjeeviChoudhary, IFS, Commissioner of Horticulture,Govt. of Andhra Pradesh on 23.12.2015. Dr.Damodar Reddy, Director, ICAR-CTRI has invitedthe students and upcoming youth to join theagricultural education in building the NationalAgricultural Research System (NARS).

Hindi Week

Hindi week was celebrated at the Instituteand its research stations from 19-26 September,2015 for the promotion of use of officiallanguage, Hindi. Sri A. Srinivasa Rao, ITS,General Manager, BSNL, Rajahmundry acted asChief Guest for the Valedictory Function andgave away the prizes to the winners of variousevents/ competitions held during the week.

Aadhar Based Biometric Attendance System

Dr. D. Damodar Reddy, Director inauguratedthe Aadhar Based Biometric Attendance Systemfor registering the staff attendance on15.08.2015.

93 ICAR - CTRI Annual Report 2015-16

National Seminar on Crop Diversification

The ICAR –CTRI has organised the NationalSeminar on Crop Diversification at Rajahmundryon 18.01.2016. The Seminar is sponsored bythe DAC & FW, Ministry of Agriculture, Govt. ofIndia. Department officials, Scientists from ICARand ANGRAU, Hyderabad and YSR HorticultureUniversity, Farmers and Industry Representativesparticipated in the Seminar and deliberated ona myriad issues relating to crop diversificationin tobacco growing areas. The National Seminarserved as a platform for exchange of informationand experiences on various issues relating tocrop diversification in tobacco growing regions.

World Soil Day

World Soil Day was celebrated on 05.12.2015at CTRI Research Station, Hunsur, Karnataka,CTRI-KVK, Kalavcharla (East Godavari dt.), A.Pand CTRI-KVK, Kandukur (Prakasam dt), A.P. Thefarmers were sensitized about the importanceof the soil health and its management. In thisconnection, 606 Soil Health Cards weredistributed to farmers of Andhra Pradesh andKarnataka.

Vigilance Awareness Week

Vigilance Awareness Week celebrated during26-31 October, 2015. Dr. D. Damodar Reddy,Director, ICAR-CTRI, Mr. T. Rama Prasada Rao,Regional vigilance & Enforcement Officer, E.Godavari, Rajahmundry, chief guest and Dr. K.Sarala, Vigilance officer addressed the staff onissues relating to Preventive Vigilance as a toolof Good Governance.

Field IRC

Field IRC was conducted at CTRI ResearchStation, Jeelugumilli on 06.02.2016 to monitorCTRi and AINPT experiments as per the approvedtechnical programme.

Field IRC was conducted at Black SoilResearch Farm, Katheru on 25.02.2016 to

monitor the experiments as per the approvedtechnical programme.

IJSC Meeting

The 2nd meeting of XIII Institute Joint StaffCouncil held on 20.11.2015 examined variousissues related to staff welfare including issueof health cards, updating of roster registers,providing facilities to IJSC members, timelyfilling up of posts, promotions etc.

Swachh Bharat Programmes

Swachh Bharat programmes were mainlyaimed at awareness creation on naturalresource management (water, soil andenergy).The programmes were implemented on‘Prevention of wastage of fuel in OfficePremises’. Swachch Bharat Programme on ‘Leafmanure making’ and ‘Eco Friendly Ganesha’were the other activities implemented.

Growers awareness meet

Growers awareness meet for MPA (ModelProjet Area) farmers on GAP, CPA and NTRMwas arranged by Tobacco Board in collaborationwith CTRI was held on 10.3.16 at CTRI RSJeelugumilli.

Scientific Advisory Committee Meeting(07.04.2015)

KVK organized SAC 2015 at CTRI,Rajahmundry on 7th April, 2015 to review workdone during 2014-15 and to finalise action planfor 2015-16.

ISO 9001:2008

ICAR-CTRI, Rajahmundry has been grantedISO 9001:2008 certificate for the successfulimplementation of its quality managementsystem with regard to “Basic, Strategic andApplied Research on Tobacco Crop” on25.01.2016.

Workshops, Seminars and Farmers’ Days organised by the Institute

ICAR - CTRI Annual Report 2015-16 94

Distinguished visitors

S.No. Date Visitors

CTRI, RAJAHMUNDRY

1. 10.04.2015 Sri B.M.C. ReddyVice Chancellor, DR. YSR H U, Venkataramanna Gudem

2. 10.04.2015 Sri K. Madhusudhan RaoIAS, Commissioner of Agrl. Govt. of Andhra Pradesh

3. 13.04.2015 Dr. K. SatyagopalIAS, Director General NIPHMRajendranagar, Hyderabad

4. 24.06.2015 Dr. Baleshi RamDirector, Sugarcane Breeding Institute,Coimbattore (TN)

5. 17.07.2015 Dr. S. AyyappanSecretary, DARE & DG, ICAR

6. 21.08.2015 Sri P. Venkateswara Rao and Team,Associate Scientist for ITC – ABD – ILTD Research Department

7. 23.09.2015 Sri Chiranjeevi ChowdharyIFS,Commissioner of Horticulture, Govt. of Andhra Pradesh (AP)

8. 31.10.2015 Dr. S. RavichandranPr. Scientist, NAARM, Hyderabad

9. 04.01.2016 Dr. J. S. ChauhanADG (Seed), ICAR, New Delhi

10. 04.01.2016 Dr. Padma RajuDr. Rama RaoVice Chancellor, ANGRAU Director, NAARM, Hyderabad

11. 07.01.2016 Sri C. RaghavachariVEVIL, BPI Pvt. Limited, ISO Auditor

12. 21.01.2016 Sri Ch. Srinivasa RaoAgrl. Officer, RARS, Lam Guntur

13. 03.02.2016 Dr. K. Manikya KumariHead, Dept. of Botany & BiotechnologySt. Joseph’s college forwomen (A) Visakhapatnam-530 004

CTRI RS, Hunsur

15. 5-12.2015 Sri. Bipin Behari ChoudahryDirector (Auctions), Tobacco Board, Bangalore

95 ICAR - CTRI Annual Report 2015-16

S.No. Date Visitors

16. 30.10. 2015 Dr. Y.P. SinghDr.A.N.Ganesh MurthyPrincipal Scientist (Agronomy), ICAR, New Delhi Head, Division of SoilScience & Agriculture Chemistry, ICAR- IIHR Bangalore

CTRI RS, Vedasandur

17. 16.05.2015 Dr. Hanchinal, Dr. R. Lakshmi Narayana, Dr. T.Ramesh Babu,Dr. R. Samantha, Dr. B.N.BhatQRT, team

18. 22.01.2016 Dr.K.Saralaand Dr.U.Sreedhar23.01.2016. Principal Scientist & Head Crop ImprovementPrincipal Scientist & Head

Crop Protection

CTRI RS, Dinhata

19. 01-07-2015 Sri Ashish Kr. Patra Deputy Director of Agriculture ,Cooch Behar

20. 21-12-2015 Sri YugeswaranandaAsstt. Secretary Ramkrishna Mission,Narendrapur, Kolkata

Distinguished visitors

ICAR - CTRI Annual Report 2015-16 96

DIVISION OF CROP IMPROVEMENT

Dr. (Mrs.) K. Sarala, Principal Scientist & HODDr. T.G.K. Murthy, Pr.Scientist &

Nodal Officer, AINPTDr. A.V.S.R. Swamy, Principal ScientistDr. K. Prabhakara Rao, ScientistK. Santhinandivelu, Sr. Technical OfficerB. Raja Rao, Technical OfficerM. Trinadh, Sr. Technical AssistantM.M. Ali, Technical AssistantM. Srinivas, Sr. TechnicianS. Ramaraju, TechnicianY.N.V.V.S.N. Murthy, SSS

DIVISION OF CROP PRODUCTION

Dr. S. Kasturi Krishna, Pr. Scientist & HODDr. S.V. Krishna Reddy, Principal ScientistDr. Y. Subbaiah, Principal ScientistDr. (Mrs.) K. Suman Kalyani, Principal ScientistN. Aruna Kumari, Chief Techl. OfficerT. Krishna Reddy, Chief Techl. OfficerI. Jagadishchandra, Sr. Techl. OfficerP.S.S Prakasa Rao, Sr. Techl. Asst.P. Girija Sankar, Sr. Technical AssistantCh. Sudhakara Babu, Techl. AssistantD.S.R. Sastry, Sr. TechnicianK. Pushpa, SSSY. Jaya Lakshmi, SSSK.V.S.S. Bhaskara Rao, SSSG. Sarveswara Rao, SSSY.V. Narayana, SSSCh. Satyanarayana, SSS

DIVISION OF CROP PROTECTION

Dr. U. Sreedhar, Principal Scientist & Head i/cDr. G. Raghupathi Rao, Sr. ScientistK. Sesha Sai, Asst. Chief Technical OfficerDr. S.K. Dam, Sr. Technical OfficerV. Narasimha Murthy, Technical OfficerV.V. Ramana, Sr. Technical AssistantA. Nageswara Rao, SSSV.V.P.L. Acharyulu, SSSB. Koteswara Rao, SSS

Dr. D. Damodar Reddy, Director

Personnel (As on 31.03.2016)

DIVISION OF CROP CHEMISTRY & SOIL SCIENCE

Dr. C. Chandrasekhara Rao, Pr. Scientist &HOD & Nodal Officer,

PME CELLDr. K. Siva Raju, Principal ScientistDr.(Mrs.) D.V. Subhashini, Principal ScientistDr. L.K. Prasad, Principal ScientistJana Poorna Bindhu, ScientistD.V.L. Satyavathi, ACTOY. Ramabai, Sr. Technical OfficerK. Padmaja, Sr. Technical OfficerJ. Vasanthi, Technical OfficerG. Srinivasa Rao, Sr. Technical AssistantN. Johnson, Technical AssistantP. Satyavathi, SSSP. Subbayamma, SSSM. Srilatha, SSSK.V. Narasimha Raju, SSSB.S.S. Sai, SSSE. Radhakrishna, SSSCh. Subba Rao, SSSA. Daniel Raju, SSSSirikonda Krishna, SSS

PME CELL

C.V.K. Reddy, Asst. Chief Techl. OfficerCh. Lakshminarayani, Personal AssistantY. Subrahmanyam, SSSN. Srinivasa Rao, SSS

ITMU

A. Aruna Kumari, Research AssociateC.V.N. Srihari, Research Associate

AKM UNIT

Dr. H. Ravisankar, Senior ScientistM.N.P. Kumar, Sr. Techl. OfficerK. Satyanarayana, SSS

LIBRARY

Md. Elias, Sr. Technical Assistant

97 ICAR - CTRI Annual Report 2015-16

Personnel

AINPT

B. Krishna Kumari, Sr. Technical OfficerR. Satyanarayana, TechnicianBathina Chinnari, SSS

MAINTENANCE SERVICE UNIT

G. Nagesh Kanth Rao, Sr. Technical OfficerN. Sreedhar, Technical OfficerN. Gopinath, Technical OfficerV.V. Sivaram, Technical OfficerG.S.N. Murthy, Technical AssistantK.V.V. Satyanarayana, Techl. AssistantN.V.V. Satyanarayana, Sr. TechnicianG. Kaleswara Rao, SSSY. Nirmala Kumari, SSS

TRANSPORT

Y. Yesu, Sr. Technical Assistant (Driver)P.Ch.S.N. Murthy, Techl. Assistant (Driver)S. Ramakrishna, Techl. Assistant (Driver)P.V.V.R. Srinivasa Rao, SSSCh. Papa, SSS

SEED PRODUCTION

G. Adinarayana, Asst. Chief Techl. OfficerGaraga S.N. Murthy, Sr. Technical AssistantB.V. Srinivas, Sr. Technical AssistantM.S. Ashokan, Techl. AssistantN. Endayya, Sr. TechnicianG. Prasada Rao, SSS

CTRI FARM, KATHERU

Dr. M. Nageswara Rao, Asst.Chief Techl.OfficerV. Madhava Rao, Technical Officer (Curer)G.H. Mohanacharyulu, Technical OfficerN. Veerabhadra Rao, AssistantK. Bhyravaswamy, Sr. Technical AssistantY.V. Subrahmanayam, Sr. TechnicianK.V. Ramana, Sr. TechnicianB. Nageswara Rao, SSSD. Balarama Reddy, SSSA. Srinivas, SSSN. Kanakanandam, SSSInapala Dharma Raju, SSSKodenagula Venkata Subba Rao, SSSUppati Kalyanam, SSS

Bagala Venkata Rama Rao, SSSYerubandi Srinivas Durga Prasad, SSSMiriyala Subba Rao, SSSPolumati Sreenivasu, SSS

BTRC, KALAVACHARLA

S. Rajeswara Rao, Sr. Technical AssistantP. Janakiramayya, SSSY.V. Subba Rao, SSSCh. Chinnayamma, SSS

ADMINISTRATION

S.L.V. Prasad, Sr. Administrative OfficerP.V.S. Bharathi, Fin. & Accounts OfficerV. Bhagyalakshmi, AAOP.V. Satyanarayana, AAON. Maheswari, Private SecretaryK.T.R. Singh, Private SecretaryN. Sambasiva Rao,Technical Officer (Gest. Opr.)V. Narayanacharyulu, AssistantV.S.R.C. Murthy, AssistantG.V.V.S. Rambabu, AssistantP. Mariyamma, AssistantM. Rambabu, AssistantA. Sridhar, AssistantN. Suryanarayana, AssistantP. Devanagaraju, AssistantA.Veera Venkata Ramana, AssistantV. Narasimha Rao, UDCP.V.V.V. Prasad, UDCCh. Jayaram, UDCP.J.F. Moses, UDCS. Pradeep Kumar, UDCP. Suchitra, UDCG.M.B. Sujatha, UDCJ. Suseela Devi, UDCY. Subbalakshmi, LDCB. Rama Rao, LDCG. Vasudeva Murthy, SSSJ. Veerraju, SSSK.A.J. Kennedy, SSSR. Sarada, SSSK. Sankurudu, SSSPeta Ramana, SSSP.K.V. Satyanarayana, SSSN. Nirmala Kumar, SSSY.S.V. Subba Rao, SSSPragada Krishna, SSSPilli Venkataramana, SSS

ICAR - CTRI Annual Report 2015-16 98

P. Kotababu, SSSG.V. Ramana, SSSKarupothu Vijaya Raju, SSS

CTRI RESEARCH STATION, GUNTUR

Dr. P. Venkateswarlu, Pr. Scientist & Head i/cB. Krishnakumar, Sr. Technical OfficerT.S.N. Murthy, Asst. Fin. & Accounts OfficerM.H. Uma, AssistantM.V. Jayakrishna, Sr. Techl. AssistantShaik Ameer Ali, Techl. AssistantA. Suresh Babu, Technical AssistantT. Nagamani, Sr. TechnicianV. Subba Rao, Sr. TechnicianS. Siva Veeraiah, LDCB. Yesu, TechnicianE. Veerabhadra Rao, TechnicianS. Sambasiva Rao, TechnicianM. Bhagyam, SSSS. Anasuya, SSSPasupuleti Raju, SSSA. Gangadhara Rao, SSSP. Purnachandra Rao, SSSManchala Prasad, SSSD.V. Rama Rao, SSSManchala Raju, SSSPathella Srinivasa Rao, SSST. Sankara Rao, SSSBandi Subbulu, SSSP. Babi Sarojini, SSSKondaveeti Subbulu, SSSSetti Subbulu, SSSCh. Sarojini, SSSS. Rajalu, SSSM. Venkayamma, SSSMandadi Anjaneyulu, SSSKanuganti Yesu, SSSD. Sreenivasa Rao, SSS

CTRI RESEARCH STATION, KANDUKUR

Dr. (Mrs.) M. Anuradha, Principal Scientist &Head

Dr. K.C. Chenchaiah, Principal ScientistDr. P.V. Venugopala Rao, Senior ScientistK. Krishna Murthy, Asst. Admn. OfficerT. Venkata Rao, Sr. Techl. Assistant (Curer)M. Mathaiah, Technical Assistant (Tr. Driver)U.R.K. Murthy, UDCK. Malakondaiah, Sr. Technician (Electrician)A. Mutyam, Sr. TechnicianK. Sudhakar, Technician

Ch. Lakshmana Rao, TechnicianM. Mohana Rao, TechnicianK. Venkateswarlu, TechnicianV. Jayamma, SSSKallagunta Kondaiah, SSSDivi Kesavulu, SSSO. Rathamma, SSSK. Mallikharjuna, SSSDama Singaiah, SSSDivi Koteswara Rao, SSSOrupalli Annapurnamma, SSSMannem Vijayamma, SSSDivi Sheshamma, SSSVankayalapati Mukundam, SSSNalluri Subbayamma, SSSDivi Annamma, SSSTanikonda Chinnamma, SSSG. Lakshmamma, SSSOrupalli Malakondaiah, SSSOruganti Lakshmamma, SSSVankayalapati Mariyamma, SSSCheemala Rangaiah, SSSDivi Malakondaiah, SSS

CTRI RESEARCH STATION, JEELUGUMILLI

K. Ganesh Babu, Asst. Admn. OfficerAnapakam Prabhu, AssistantT. Lakshmana Rao, Sr. Technical AssistantT. Srinivasa Rao, Technical AssistantY. Venkateswara Rao, Technical AssistantD.V. Prasad, Technical AssistantJ. Hanumantha Rao, Technical AssistantA.L.N. Murthy, Sr. TechnicianB. Durga Rao, Sr. TechnicianG. Govinda Raju, Sr. TechnicianD. Yesuratnam, TechnicianP.V. Ramana, TechnicianP. Kumar, TechnicianP.Y. Narasimha Rao, SSS

CTRI RESEARCH STATION, HUNSUR

Dr. S. Ramakrishnan, Pr.Scientist & Head i/cDr. M. Mahadevaswamy, Principal ScientistDr. (Mrs.) C. Nanda, ScientistS. Ramesh, Chief Technical OfficerC. Mahadeva, Chief Technical OfficerDr. P. Nagesh, Asst. Chief Technical OfficerDr. S.S. Srinivas, Asst. Chief Technical OfficerN.D. Suresh, Sr. Technical OfficerT. Venkatesh, Sr. Technical OfficerS.V. Ramana, Asst. Administrative Officer

Personnel

99 ICAR - CTRI Annual Report 2015-16

Personnel

P. Prabhakara Murthy, AssistantRohini, AssistantSambu Gowda, Sr. Technical AssistantChikkanna Setti, Sr. Technical AssistantSanna Swamy, Sr. Technical AssistantD.K. Eswara, Sr. Technical AssistantGopala Rao, Technical AssistantB. Suresh Kumar, Technical AssistantC. Puttamallaiah, Technical AssistantDevamma, SSSChikkabhagya, SSSJayamma, SSSByre Gowda, SSS

CTRI RESEARCH STATION,VEDASANDUR

Dr. M. Kumaresan, Principal Scientist &Head i/c

R. Rajendran, Sr. Technical OfficerC. Muruganandam, Sr. Technical OfficerV. Annadurai, Sr. Technical OfficerS. Soundararajan, Technical OfficerR. Indrani, TechnicianN. Manjula, AssistantC. Nehru, AssistantP. Malliasamy, SSSN. Rosammal Punnus, SSSM. Pitchaiammal, SSSR. Kennedy Balasubramanian, SSS

CTRI RESEARCH STATION, DINHATA

Sunil Mandi, Scientist & Head i/cJ.K. Roy Barman, Technician

S.K. Sarkar, Personal AssistantB.N. Ghosh, AssistantM. Mumtaz Begum, UDCGowtham Ghosh, LDCN.R. Barman, SSSA.K. Chisim, SSSUttam Das, SSSGauranga Sarkar, SSSNaresh Biswas, SSS

KVK, KALAVACHARLA

Dr. V.S.G.R. Naidu, Programme CoordinatorV.V. Lakshmikumari, Chief Technical OfficerDr. B. John Babu, Chief Technical OfficerR. Sudhakar, Chief Technical OfficerJ.V.R. Satyavani, Chief Technical OfficerE. Vijaya Prasad, Chief Technical OfficerDr. P.V.V.S. Siva Rao, Asst. Chief

Technical OfficerM. Ramamohana Rao, Asst. Admn. OfficerT. Syamala Devi, AssistantD. Balakrishna, Technical OfficerP. Eswara Rao, Technical Assistant (Driver)Y. Udayakumar, Sr. Technician (Tr. Driver)G. Chinna Rao, SSSG. Sasi Rani, SSSG.P.D. Varma, SSSM. Veeraveni, SSSG. Ramakrishna Raju, SSSKamidi Venkanna, SSSPenumakula Surya Kumari, SSSChalla Koteswara Rao, SSSSadanala Nageswara Rao, SSS

ICAR - CTRI Annual Report 2015-16 100

Address : Rajahmundry-533105, Andhra Pradesh

Website ID: www.ctri.org.in

Section 1: Vision, Mission, Objectives and Functions

Vision

Enhancing productivity and quality of Indian tobacco to make it more remunerative, globallycompetitive and promoting alternative uses to sustain the crop in the country.

Mission

Developing economically viable and eco-friendly agro-technologies for enhancing productivityand quality, reducing harmful substances, developing value-added products for promoting exportsand generating revenue and employment on a sustainable basis.

Objectives

1. Germplasm enhancement and development of improved cultivars2. Development and identification of appropriate technologies3. Technology dissemination and capacity building

Functions

1. To conduct research on different types of tobacco, with greater emphasis on exportabletypes, on all phases of production management with a view of attaining economic advantage/benefit to the tobacco growers through improvement in quality and quantity of tobacco

2. To conduct research on economically viable and sustainable cropping systems alternative totobacco

3. To conduct research on diversified uses of tobacco and development of value-added productssuch as phyto-chemicals, oil, biomass based products etc.

4. To produce and distribute quality seeds of released varieties of tobacco

5. To publish and disseminate research findings and recommendations of latest technology forthe benefit of the tobacco growers, scientific community, policy makers and developmentagencies

Appendix - 1

Results-Framework Document for ICAR-Central Tobacco Research Institute (2014-2015)

101 ICAR - CTRI Annual Report 2015-16

Sect

ion

2: I

nter

se

prio

riti

es a

mon

g Ke

y O

bjec

tive

s, S

ucce

ss In

dica

tors

and

Tar

gets

S.

N

o.

Obj

ecti

ves

Wei

ght

A

ctio

ns

Succ

ess

Indi

cato

rs

Uni

t W

eigh

t

Targ

et/C

rite

ria

Val

ue

Exce

llent

Very

Goo

d G

ood

Fair

Poor

100%

90

%80

% 70

% 60

% 1.

G

erm

plas

m

enha

ncem

ent

and

deve

lopm

ent

of

impr

oved

cul

tiva

rs

35

Eval

uati

on o

f ge

neti

c m

ater

ial

Bree

ding

and

ge

rmpl

asm

line

s ev

alua

ted

Num

ber

13

132

110

88

66

44

Line

s id

enti

fied

fo

r u

niqu

e tr

aits

D

ate

3 1st

wee

k of

Se

pt.,

20

14

1st w

eek

of

Oct

., 2

014

1st w

eek

of

Nov

., 2

014

1st w

eek

of

Dec

., 2

014

1st w

eek

of

Jan.

, 20

15

Dev

elop

men

t of

im

prov

ed c

ulti

vars

En

trie

s te

sted

in

AIN

P(T)

mul

ti-

loca

tion

tri

als

Num

ber

7 26

22

18

14

10

Vari

etie

s id

enti

fied

fo

r re

leas

e

Num

ber

4 3

2 1

0 0

Seed

pro

duct

ion

prog

ram

me

Foun

dati

on s

eed

prod

uced

W

eigh

t M

T 8

20

19

15

11

7

2.

Dev

elop

men

t an

d id

enti

fica

tion

of

appr

opri

ate

tech

nolo

gies

27

Dev

elop

men

t of

new

te

chno

logi

es

New

tec

hnol

ogie

s te

sted

/dev

elop

ed

Num

ber

27

6 5

43

2

3.

Tech

nolo

gy

diss

emin

atio

n an

d ca

paci

ty b

uild

ing

18

Dem

onst

rati

ons

cond

ucte

d D

emon

stra

tion

s/

FLD

s co

nduc

ted

Num

ber

9 12

10

8

6 4

Trai

ning

pro

gram

mes

or

gani

zed

for

farm

ers/

Exte

nsio

n of

fici

als

Trai

ning

s or

gani

zed

Num

ber

9 14

12

10

7

4

* Pu

blic

atio

n/D

ocum

ent

atio

n 5

Publ

icat

ion

of t

he

rese

arch

art

icle

s in

the

jo

urna

ls h

avin

g th

e N

AAS

rati

ng o

f 6.

0 an

d ab

ove

Rese

arch

art

icle

s pu

blis

hed

No.

3 6

5 4

3 2

Tim

ely

publ

icat

ion

of

the

Inst

itut

e An

nual

Re

port

(20

13-2

014)

Annu

al R

epor

t pu

blis

hed

Dat

e2

30.0

6.20

14

02.0

7.20

14

04.0

7.20

14

07.0

7.20

14

09.0

7.20

14

* Fi

scal

res

ourc

e m

anag

emen

t 2

Uti

lizat

ion

of r

elea

sed

plan

fun

d Pl

an f

und

utili

zed

%

2 98

96

94

92

90

* Ef

fici

ent

Func

tion

ing

of t

he R

FD S

yste

m

3Ti

mel

y su

bmis

sion

of

Dra

ft R

FD f

or 2

014-

2015

for

App

rova

l

On-

tim

e su

bmis

sion

D

ate

2

May

15,

20

14

May

16,

20

14

May

19,

20

14

May

20,

20

14

May

21,

20

14

ICAR - CTRI Annual Report 2015-16 102

Enha

nced

Tr

ansp

aren

cy /

Im

prov

ed S

ervi

ce

deliv

ery

of

Min

istr

y/De

part

men

t

3 Ra

ting

fro

m

Inde

pend

ent

Audi

t of

im

plem

enta

tion

of

Citi

zens

’ /

Clie

nts’

Ch

arte

r (C

CC)

Deg

ree

of

impl

emen

tati

on o

f co

mm

itm

ents

in

CCC

%

210

095

90

85

80

Inde

pend

ent

Audi

t of

im

plem

enta

tion

of

Gri

evan

ce R

edre

ss

Man

agem

ent

(GRM

) sy

stem

Deg

ree

of s

ucce

ss

in im

plem

enti

ng

GRM

%

110

095

90

85

80

Adm

inis

trat

ive

Refo

rms

7

Upd

ate

orga

niza

tion

al

stra

tegy

to

alig

n w

ith

revi

sed

prio

riti

es

Dat

e

Dat

e

2N

ov.1

201

4N

ov.2

201

4 N

ov.3

201

4 N

ov.4

201

4N

ov.5

201

4

Impl

emen

tati

on o

f ag

reed

mile

ston

es o

f ap

prov

ed M

itig

atin

g St

rate

gies

for

Red

ucti

on

of p

oten

tial

ris

k of

co

rrup

tion

(M

SC).

% of

Im

plem

enta

tion

%

1

100

90

80

7060

Impl

emen

tati

on o

f ag

reed

mile

ston

es f

or

ISO

900

1

% of

im

plem

enta

tion

%

2

100

95

90

8580

Impl

emen

tati

on o

f m

ilest

ones

of

appr

oved

In

nova

tion

Act

ion

Plan

s (I

APs)

.

% of

im

plem

enta

tion

%

2

100

90

80

7060

103 ICAR - CTRI Annual Report 2015-16

Sect

ion

3: T

rend

Val

ues

of t

he S

ucce

ss In

dica

tors

S.

N

o.

Obj

ecti

ves

Act

ions

Su

cces

s In

dica

tors

U

nit

Act

ual

Val

ue

for

FY

12/1

3

Act

ual

Val

ue

for

FY

13/1

4

targ

etV

alue

for

FY

14/1

5

Proj

ecte

d V

alue

for

FY

15/

16

Proj

ecte

d V

alue

for

FY

16/

17

1.

Ger

mpl

asm

enh

ance

men

t an

d de

velo

pmen

t of

im

prov

ed c

ulti

vars

Eval

uati

on o

f ge

neti

c m

ater

ial

Bree

ding

and

ger

mpl

asm

lin

es e

valu

ated

N

umbe

r98

100

110

112

115

Line

s id

enti

fied

for

uni

que

trai

ts

Num

ber

1st

wee

k of

Se

pt.,

20

14

1st wee

k of

Se

pt.,

20

14

1st w

eek

of

Oct

., 2

014

1st w

eek

of

Oct

., 2

014

1st w

eek

of

Oct

., 2

014

Dev

elop

men

t of

impr

oved

cu

ltiv

ars

Entr

ies

test

ed in

AIN

RP(T

) m

ulti

-loc

atio

n tr

ials

N

umbe

r15

20

22

23

24

Vari

etie

s id

enti

fied

fo

r re

leas

e N

umbe

r3

1 2

3 4

Seed

pro

duct

ion

prog

ram

me

Foun

dati

on s

eed

prod

uced

Wei

ght

MT

1918

.5

19

19.5

20

2.

Dev

elop

men

t an

d id

enti

fica

tion

of

appr

opri

ate

tech

nolo

gies

Dev

elop

men

t of

new

te

chno

logi

es

New

tec

hnol

ogie

s te

sted

/dev

elop

ed

Num

ber

44

56

7

3.

Tech

nolo

gy d

isse

min

atio

n an

d ca

paci

ty b

uild

ing

Dem

onst

rati

ons

cond

ucte

d D

emon

stra

tion

s /

FLD

s co

nduc

ted

Num

ber

99

10

10

10

Trai

ning

pro

gram

mes

or

gani

zed

for

farm

ers/

Exte

nsio

n of

fici

als

Trai

ning

s or

gani

zed

Num

ber

1112

12

13

14

* Pu

blic

atio

n/D

ocum

enta

tion

Pu

blic

atio

n of

the

res

earc

h ar

ticl

es in

the

jou

rnal

s ha

ving

the

NAA

S ra

ting

of

6.0

and

abov

e

Rese

arch

art

icle

s pu

blis

hed

No.

5

4 5

6 7

Tim

ely

publ

icat

ion

of t

he

Inst

itut

e An

nual

Rep

ort

(201

3-20

14)

Annu

al R

epor

t pu

blis

hed

Dat

e

02.0

7.20

14

* Fi

scal

res

ourc

e m

anag

emen

t U

tiliz

atio

n of

rel

ease

d pl

an

fund

Pl

an f

und

utili

zed

%

100

100

100

100

100

* Ef

fici

ent

Func

tion

ing

of t

he

RFD

Sys

tem

Ti

mel

y su

bmis

sion

of

Dra

ft

RFD

for

201

4-20

15 f

or

Appr

oval

On-

tim

e su

bmis

sion

D

ate

- -

May

16,

201

4 -

-

Tim

ely

subm

issi

on o

f Re

sult

s fo

r 20

13-2

014

O

n-ti

me

subm

issi

on

Dat

e -

- M

ay 2

20

14

- -

ICAR - CTRI Annual Report 2015-16 104

* En

hanc

ed T

rans

pare

ncy

/ Im

prov

ed S

ervi

ce d

eliv

ery

of M

inis

try/

Dep

artm

ent

Rati

ng f

rom

Inde

pend

ent

Audi

t of

impl

emen

tati

on o

f Ci

tize

ns’

/ Cl

ient

s’ C

hart

er

(CCC

)

Deg

ree

of im

plem

enta

tion

of

com

mit

men

ts in

CCC

%

- -

95

- -

Inde

pend

ent

Audi

t of

im

plem

enta

tion

of

Gri

evan

ce R

edre

ss

Man

agem

ent

(GRM

) sy

stem

Deg

ree

of s

ucce

ss in

im

plem

enti

ng G

RM

% -

- 95

-

-

* Ad

min

istr

ativ

e Re

form

s

Upd

ate

orga

niza

tion

al

stra

tegy

to

alig

n w

ith

revi

sed

prio

riti

es

Dat

e

Dat

e -

- N

ov.2

201

4 -

-

Impl

emen

tati

on o

f ag

reed

m

ilest

ones

of

appr

oved

M

itig

atin

g St

rate

gies

for

Re

duct

ion

of p

oten

tial

ris

k of

cor

rupt

ion

(MSC

)

% of

Impl

emen

tati

on

% -

- 90

-

-

Impl

emen

tati

on o

f ag

reed

m

ilest

ones

for

ISO

900

1

% of

impl

emen

tati

on

% -

- 95

-

-

Impl

emen

tati

on o

f m

ilest

ones

of

appr

oved

In

nova

tion

Act

ion

Plan

s (I

APs)

% of

impl

emen

tati

on

% -

- 90

-

-

Sect

ion

4 (a

): A

cron

yms

Sl.N

o.A

cron

ymD

escr

ipti

on1

AIN

RP(T

)Al

l In

dia

Net

wor

k Re

sear

ch P

roje

ct o

n To

bacc

o2

FLD

Fron

t Li

ne D

emon

stra

tion

3FC

VFl

ue-c

ured

Vir

gini

a3

MT

Mill

ion

tons

105 ICAR - CTRI Annual Report 2015-16

Sect

ion

4 (b

):D

escr

ipti

on a

nd d

efin

itio

n of

suc

cess

ind

icat

ors

and

prop

osed

mea

sure

men

t m

etho

dolo

gy

Sl.N

o.

Succ

ess

Indi

cato

r D

escr

ipti

on

Def

init

ion

Mea

sure

men

tG

ener

al c

omm

ents

1

Bree

ding

and

ge

rmpl

asm

line

s ev

alua

ted

Sour

ce m

ater

ial

for

the

impr

oved

var

ieti

es

to b

e ev

alua

ted

Mat

eria

l ge

nera

ted

from

th

e ba

sic

germ

plas

m

Num

ber

of

bree

ding

lin

es e

valu

ated

2 Li

nes

iden

tifi

ed f

or

uniq

ue t

rait

s G

erm

plas

m li

nes

havi

ng u

niqu

e tr

aits

G

erm

plas

m l

ine

or m

ater

ial

gene

rate

d fr

om b

asic

ger

mpl

asm

D

ate

of i

dent

ific

atio

n of

lin

e ha

ve

uniq

ue

trai

ts

3 En

trie

s te

sted

in

AIN

P(T)

mul

ti-l

ocat

ion

tria

ls

Impr

oved

lin

es

deve

lope

d in

br

eedi

ng

prog

ram

mes

Ad

vanc

ed b

reed

ing

lines

hav

ing

high

er

yiel

d an

d qu

alit

y/re

sist

ance

/tol

eran

ce

to b

ioti

c an

d ab

ioti

c st

ress

es

Num

ber

of

entr

ies

test

ed

4 Va

riet

ies

iden

tifi

ed

for

rele

ase

Bree

ding

lin

es t

este

d al

ong

wit

h ch

ecks

in

mul

ti-l

ocat

ion

tria

ls t

hrou

gh A

INP(

T) a

nd

the

best

pe

rfor

min

g en

trie

s co

mpa

red

to

chec

ks

are

iden

tifi

ed

as

new

im

prov

ed

vari

etie

s fo

r re

leas

e

Best

per

form

ing

entr

ies

iden

tifi

ed a

s a

new

var

iety

for

rel

ease

dur

ing

Inst

itut

e Re

sear

ch

Com

mit

tee

mee

ting

or

AI

NRP

T gr

oup

mee

ting

/wor

ksho

p

Num

ber

of

vari

etie

s id

enti

fied

Id

enti

fica

tion

of

va

riet

ies

depe

nd u

pon

the

avai

labi

lity

of

supe

rior

m

ater

ial

wit

h re

spec

t to

yie

ld b

ioti

c an

d ab

ioti

c re

sist

ance

/tol

eran

ce

over

the

exi

stin

g va

riet

ies

5 Fo

unda

tion

see

d pr

oduc

ed

Prod

ucti

on o

f br

eede

r or

fou

ndat

ion

seed

pr

oduc

ed u

nder

the

str

ict

supe

rvis

ion

of

scie

ntis

ts

Foun

dati

on

seed

in

to

bacc

o is

th

e st

arti

ng p

oint

in

the

seed

cha

in w

hich

is

m

ulti

plie

d fo

r di

stri

buti

on

amon

g fa

rmer

s

Qua

ntit

y pr

oduc

ed

(Qui

ntal

s)

Qua

ntit

y m

ay

vary

as

pe

r th

e in

dent

fr

om

Toba

cco

Boar

d/fa

rmer

req

uire

men

t

6 N

ew t

echn

olog

ies

test

ed/d

evel

oped

Te

chno

logi

es

deve

lope

d ar

e ai

med

to

in

crea

se y

ield

and

qua

lity

and

to r

educ

e th

e co

st

of

cult

ivat

ion

and

pest

icid

e re

sidu

es

Tech

nolo

gy r

efer

s to

new

or

mod

ifie

d pr

acti

ce w

hich

can

be

used

as

one

of

the

Goo

d Ag

ricu

ltur

e P

ract

ice

Num

ber

This

w

ill

incr

ease

th

e pr

oduc

tivi

ty

and

prof

itab

ility

7 D

emon

stra

tion

s/ F

LDs

cond

ucte

d Tr

ials

and

dem

onst

rati

ons

cond

ucte

d fo

r te

chno

logy

te

stin

g an

d pr

ovin

g th

e te

chno

logy

pro

duct

ion

pote

ntia

l

Dem

onst

rati

ng t

he p

rodu

ctio

n po

tent

ial

of

the

tech

nolo

gy

unde

r re

al

farm

si

tuat

ion

and

to r

ecei

ve f

eed

back

fro

m

the

stak

ehol

ders

Num

ber

of

dem

onst

rati

ons/

FL

Ds

cond

ucte

d

FLD

s w

ill b

e pr

opos

ed b

ased

on

the

ava

ilabl

e in

nova

tion

s

8 Tr

aini

ngs

orga

nize

dTh

e kn

owle

dge

and

skill

s of

the

pri

mar

y an

d se

cond

ary

stak

ehol

ders

sh

all

be

enha

nced

by

org

aniz

ing

trai

ning

s to

th

e co

ncer

ned

Trai

ning

is t

he le

arni

ng p

roce

ss t

hrou

gh

whi

ch

know

ledg

e is

im

prov

ed,

atti

tude

s ar

e ch

ange

d an

d sk

ills

are

shar

pene

d fo

r en

hanc

ing

the

perf

orm

ance

of

cl

ient

ele

(far

mer

s,

exte

nsio

n fu

ncti

onar

ies

etc.

)

Num

ber

of

trai

ning

s or

gani

zed

Nee

d ba

sed

on/o

ff c

ampu

s tr

aini

ng p

rogr

amm

es w

ill b

e or

gani

zed

ICAR - CTRI Annual Report 2015-16 106

Sect

ion

5: S

peci

fic

perf

orm

ance

req

uire

men

ts f

rom

oth

er d

epar

tmen

ts t

hat

are

crit

ical

for

del

iver

ing

agre

ed r

esul

ts

Loca

tion

ty

pe

Stat

e O

rgan

izat

ion

Type

O

rgan

isat

ion

Nam

e Re

leva

nt S

ucce

ss

Indi

cato

r W

hat

is y

our

requ

irem

ent

from

th

is o

rgan

izat

ion

Just

ific

atio

n fo

r th

is

requ

irem

ent

Plea

se q

uant

ify

your

re

quir

emen

t fr

om t

his

Org

anis

atio

n

Wha

t ha

ppen

s if

you

r re

quir

emen

t is

no

t m

et

Cent

ral

Gov

ernm

ent

Bo

ard

To

bacc

o Bo

ard

Foun

dati

on s

eed

prod

uced

In

dent

for

qua

ntit

y of

tob

acco

see

d fo

r di

stri

buti

on

Toba

cco

boar

d fi

xes

the

crop

si

ze e

very

yea

r fo

r FC

V to

bacc

o.

FCV

seed

inde

nt

vari

es b

ased

on

the

area

fix

ed

Qua

ntit

y of

fo

unda

tion

see

d re

quir

ed

Qua

ntit

y of

fo

unda

tion

see

d pr

oduc

ed m

ay

fluc

tuat

e

Sect

ion

6 :

Out

com

e/ Im

pact

of

acti

viti

es o

f D

epar

tmen

t /

Min

istr

y

S.N

o.

Out

com

e/Im

pact

of

orga

niza

tion

Join

tly

resp

onsi

ble

foin

flue

ncin

g th

is

outc

ome/

impa

ct w

ith

the

follo

win

g or

gani

zati

on(s

) de

part

men

ts/

min

istr

y(ie

s)

Succ

ess

Indi

cato

r (s

) U

nit

2012

-20

13

2013

-20

14

2014

-20

15

2015

-20

16

2016

-20

17

1.

Enha

ncem

ent

in f

arm

pr

oduc

tion

& p

rodu

ctio

n ef

fici

ency

Toba

cco

Boar

d,

Min

istr

y of

Co

mm

erce

and

to

bacc

o In

dust

ry

Incr

ease

d fa

rm

prod

ucti

on

M.

kg

9.5

10

10

10

10

Incr

ease

in f

arm

in

com

e Ru

pees

(Mill

ion)

36

0 37

0 38

0 38

539

0

Incr

ease

d fa

rm

prod

ucti

vity

Kg

/ha

33.3

234

.334

.67

35.2

8 35

.44

107 ICAR - CTRI Annual Report 2015-16

Ann

ual

(A

pril

1, 2

014

to M

arch

31,

201

5) P

erfo

rman

ce E

valu

atio

n Re

port

of

RFD

of

RSCs

i.e

. In

stit

utio

ns f

or t

he y

ear

2014

-201

5

S.

N

o.

Obj

ecti

ves

Wei

ght

A

ctio

ns

Succ

ess

Indi

cato

rs

Uni

t W

eigh

t

Targ

et/C

rite

ria

Valu

eA

chie

vem

ents

Pe

rfor

man

cePe

rcen

t ac

hiev

emen

ts

agai

nst

Targ

et v

alue

s of

90%

Col

.

Reas

ons

for

shor

tfal

ls

or e

xces

sive

ac

hiev

emen

ts,

if

appl

icab

le

Exce

llent

Ve

ry G

ood

Goo

dFa

ir

Poor

100%

90%

80%

70

%60

%Ra

w

Scor

e W

eigh

ted

Scor

e

1.

Ger

mpl

asm

en

hanc

emen

t an

d de

velo

pmen

t of

im

prov

ed

cult

ivar

s

35

Eval

uati

on o

f ge

neti

c m

ater

ial

Bree

ding

an

d ge

rmpl

asm

lin

es

eval

uate

d

Num

ber

13

132

110

88

66

44

144

100

13

130.

9

Num

ber

of

entr

ies

eval

uate

d in

crea

sed

due

to

the

avai

labi

lity

of

entr

ies

wit

h de

sira

ble

char

acte

rs

sele

cted

at

C

TRI

&

its

Rese

arch

St

atio

ns.

Line

s id

enti

fied

fo

r u

niqu

e tr

aits

Dat

e

3 2

1 1

1 1

3 10

0 3

300.

0

The

achi

evem

ent

is o

ne

high

er t

han

the

100%

of

the

crit

eria

onl

y.

Dev

elop

men

t of

im

prov

e-ed

cul

tiva

rs

Entr

ies

test

ed in

AI

NP(

T)

mul

ti-

loca

tion

tr

ials

Num

ber

7 26

22

18

14

10

30

10

0 7

136.

4

Num

ber

of

entr

ies

test

ed

incr

ease

d du

e co

ntri

buti

on

of

mor

e nu

mbe

r of

ent

ries

hav

ing

high

er

yiel

d w

ith

desi

rabl

e ch

arac

ters

by

AI

NPT

Cen

tres

. Va

riet

ies

iden

tifi

ed

for

rele

ase

Num

ber

4 3

2 1

0 0

4 10

0 4

200.

0

The

achi

evem

ent

is o

ne

mor

e th

an 1

00%

of

the

crit

eria

onl

y.

Seed

pro

duct

ion

prog

ram

me

Foun

dati

on

seed

pr

oduc

ed

Wei

ght

MT

8 20

19

18

17

16

16

60

4.

8 84

.2

The

nece

ssar

y se

ed

requ

irem

ent

met

w

ith

seed

pro

duce

d.

2.

Dev

elop

men

t an

d id

enti

fica

tio

n of

ap

prop

riat

e te

chno

logi

es

27

Dev

elop

men

t of

new

te

chno

logi

es

New

te

chno

logi

es te

sted

/dev

elop

ed

Num

ber

27

6 5

4 3

2 6

100

27

120.

0

The

achi

evem

ent

is 1

00%

of

the

cri

teri

a on

ly.

3.

Tech

nolo

gy

diss

emin

atio

n an

d ca

paci

ty

build

ing

18

Dem

onst

rati

ons

cond

ucte

d D

emon

stra

tio

ns/

FLD

s co

nduc

ted

Num

ber

9 12

10

8

6 4

12

100

9 12

0.0

The

achi

evem

ent

is 1

00%

of

the

cri

teri

a on

ly.

Trai

ning

pro

gram

mes

or

gani

zed

for

farm

ers/

Ext

ensi

on

offi

cial

s

Trai

ning

s or

gani

zed

Num

ber

9 14

12

10

8

6 14

10

0 9

116.

7

The

achi

evem

ent

is 1

00%

of

the

cri

teri

a on

ly.

* Pu

blic

atio

n/D

ocum

enta

tion

5 Pu

blic

atio

n of

the

re

sear

ch a

rtic

les

in

the

jour

nals

hav

ing

the

NAA

S ra

ting

of

6.

0 an

d ab

ove

Rese

arch

ar

ticl

es

publ

ishe

d

No.

3 6

5 4

3 2

6 10

0 3

120.

0

The

achi

evem

ent

is 1

00%

of

the

cri

teri

a on

ly.

Tim

ely

publ

icat

ion

of

the

Inst

itut

e An

nual

Re

port

(20

13-2

014)

Annu

al

Repo

rt

publ

ishe

d

Dat

e

2 30

.06.

201

4 02

. 07

. 20

14

04.0

7.14

07

.07.

20

14

09.0 7.

2014

15.0

6.20

14

100

2

ICAR - CTRI Annual Report 2015-16 108

* Fi

scal

re

sour

ce

man

agem

ent

2 U

tiliz

atio

n of

re

leas

ed p

lan

fund

Pl

an f

und

utili

zed

%

2 98

96

94

92

90

10

0 10

0 2

* Ef

fici

ent

Func

tion

ing

of t

he R

FD

Syst

em

3 Ti

mel

y su

bmis

sion

of

Dra

ft R

FD f

or 2

014-

2015

for

App

rova

l

On-

tim

e su

bmis

sion

D

ate

2 M

ay 1

5,

2014

M

ay 1

6,

2014

M

ay

19,

2014

May

20

, 20

14

May

21

, 20

14

M

ay 2

8,

2014

0

0

Tim

ely

subm

issi

on o

f Re

sult

s fo

r 20

13-2

014

O

n-ti

me

subm

issi

on

Dat

e

1

May

1

2014

M

ay 2

20

14

May

5 20

14

May

6

2014

M

ay

7 2014

Apri

l 25

2014

10

0 1

* En

hanc

ed

Tran

spar

enc

y /

Impr

oved

Se

rvic

e de

liver

y of

M

inis

try/

Dep

artm

ent

3 Ra

ting

fro

m

Inde

pend

ent

Audi

t of

impl

emen

tati

on

of C

itiz

ens’

/

Clie

nts’

Cha

rter

(C

CC)

Deg

ree

of

impl

emen

tati

on o

f co

mm

itm

ents

in C

CC

%

2 10

0 95

90

85

80

10

0 10

0 2

Inde

pend

ent

Audi

t of

impl

emen

tati

on

of G

riev

ance

Red

ress

M

anag

emen

t (G

RM)

syst

em

Deg

ree

of

succ

ess

in

impl

emen

ting

GR

M

%

1 10

0 95

90

85

80

10

0 10

0 1

* Ad

min

istr

ati

ve R

efor

ms

7

Upd

ate

orga

niza

tion

al

stra

tegy

to

alig

n w

ith

revi

sed

prio

riti

es

Dat

e

Dat

e

2 N

ov.

1 20

14

Nov

.2

2014

N

ov.3

20

14

Nov

.4

2014

N

ov.

5 20

14

Oct

, 31

, 20

14

100

2

Impl

emen

tati

on o

f ag

reed

mile

ston

es o

f ap

prov

ed M

itig

atin

g St

rate

gies

for

Re

duct

ion

of

pote

ntia

l ris

k of

co

rrup

tion

(M

SC).

% o

f Im

plem

enta

tion

%

1 10

0 90

80

70

60

10

0 10

0 1

Impl

emen

tati

on o

f ag

reed

mile

ston

es

for

ISO

900

1

% o

f im

plem

enta

tion

%

2 10

0 95

90

85

80

--

--

0

Impl

emen

tati

on o

f m

ilest

ones

of

appr

oved

Inno

vati

on

Acti

on P

lans

(IA

Ps).

% o

f im

plem

enta

tion

%

2 10

0 90

80

70

60

10

0 10

0 2

Tota

l Co

mpo

site

Sco

re:

92.

80%

Rati

ng :

Ver

y go

od

ix ICAR - CTRI Annual Report 2015-16

ICAR - CTRI Annual Report 2015-16 x