Excel S2_SILAC data_HEK293T.xlsx

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Supporting Information

Live-Cell Imaging and Profiling of c-Jun N-Terminal Kinases with Covalent

Inhibitor-Derived Probes

Linghui Qian,a,b* Sijun Pan,a Jun-Seok Lee,c Jingyan Ge,d Lin Li,e* Shao Q. Yaoa*

aDepartment of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543

(Singapore), E-mail: [email protected] (S.Q.Y.).

bInstitute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences,

Zhejiang University, Hangzhou 310058 (China), E-mail: [email protected] (L.Q.)

cMolecular Recognition Research Center, Bio-Med Program of KIST-School UST, Korea Institute of

Science & Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792 (South Korea)

dKey Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and

Bioengineering, Zhejiang University of Technology, Hangzhou 310014 (China)

eKey Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing

Tech University (NanjingTech), 30 South Puzhu Road, Nanjing 211800 (China), E-mail:

[email protected] (L.L.)

Electronic Supplementary Material (ESI) for ChemComm.This journal is © The Royal Society of Chemistry 2018

mailto:[email protected]



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List of contents:

1 General Information 2 Chemical Synthesis and Characterizations 3 Experimental Methods 4 Results and Discussions 5 Appendix (1H and 13C NMR spectra & HRMS Data) 6 LC-MS/MS Data for Chemoproteomic Studies 7 Summary of performance of the probes and reporters Fig. S1

8 LC-MS characterization of QT1/QT2 + trans-cyclooctenol (TCO) ligation Fig. S2

9 Characterization of AAN-N3 + propargyl alcohol ligation Fig. S3

10 Photophysical properties of two-photon reporters before and after click reaction

Fig. S4

11 Bioactivity of parental inhibitors and probes derived from them Fig. S5

12 Concentration-dependent in situ reactivity profiles of JP3/JP2-TCO in JNK1-GFP-transfected HeLa cells

Fig. S6

13 Cellular imaging using small-molecule probes/two-photon reporters for JNKs Fig. S7

14 In situ profiling of endogenous JNKs in HeLa/HEK293T/SH-SY5Y cells upon labeling by JP3/JP2-TCO

Fig. S8

15 Summary of probes and reporters used in the current study Table S1

16 Photophysical properties of fluorogenic reporters (QT1, QT2 and AAN-N3) before and after click reaction

Table S2

17 Summary of putative hits identified from standard PD/LC-MS by using 1 μM of JP3/JP2-TCO/JP2-CP for 1 h in anisomycin-induced (2 μM, 0.5 h) HeLa cells

Table S3

18 Design of bioorthogonal small-molecule probes for JNKs (JP2-TCO/JP2-CP/JP3) based on the irreversible inhibitor selective for JNKs (JNK-IN-8)

Scheme S1

19 Synthesis of small-molecule probes and reporters for JNKs Scheme S2

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1. General Information

All chemicals were purchased from commercial vendors and used without further purification, unless otherwise

noted. All non-aqueous reactions were carried out under a nitrogen/argon atmosphere in oven-dried glassware. Reaction

progress was monitored by TLC on pre-coated silica plates (Merck 60 F254 nm, 250 μm thickness) and spots were visualized by UV light or appropriate staining (e.g., ceric ammonium molybdate (CAM), basic KMnO4). Flash column

chromatography was carried out using silica gel (Merck 60 F254 nm 0.040-0.063 μm). All 1H NMR and 13C NMR spectra

were taken on a Bruker AFC 300 (300 MHz) or AMX500 (500 MHz) spectrometer. Chemical shifts were reported in

parts per million (ppm) relative to residual solvent peaks (CDCl3 = 7.26 ppm, DMSO-d6 = 2.50 ppm, CD3OD = 3.31 ppm,

Acetone-d6 = 2.05 ppm). 1H NMR coupling constants (J) are reported in Hertz (Hz) and multiplicity is indicated as

follows: s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), br s (broad singlet), br d (broad doublet), dd (doublet

of doublet), dt (doublet of triplet), dq (doublet of quartet), tq (triplet of quartet). Analytical HPLC and Mass spectra were

recorded on a Shimadzu LC-IT-TOF spectrometer or LC-ESI spectrometer equipped with an autosampler, using

reverse-phase Phenomenex Luna 5 μm C18 100 Å 50 × 3.0 mm columns. UV-vis absorption and fluorescence spectra

were measured by using a Shimadzu UV-vis spectrophotometer and a Perkin Elmer LS50 spectrofluorometer,

respectively. The two-photon excited fluorescence measurements were performed using a Spectra Physics femtosecond

Ti:sapphire oscillator (Tsunami) as the excitation source. The output laser pulses have a tunable center wavelength at 760

nm with pulse duration of 40 fs and a repetition rate of 76 MHz. The laser beam was focused onto the sample that was

contained in a cuvette with path length of 1 cm. The emission from the sample was collected at 90° angle by a pair of

lenses and an optical fiber that was connected to a monochromator (Acton, Spectra Pro 2300i) coupled with CCD system

(Princeton Instruments, Pixis 100B). A short pass filter with cut-off wavelength at 700 nm was placed before the

spectrometer to minimize the scattering from the pump beam. All optical measurements were performed at room

temperature. All 2D images were acquired on Leica TCS SP5X Confocal Microscope System equipped with Leica HCX

PL APO 63x/1.20 W CORR CS, 405 nm Diode laser, Argon ion laser, white laser (470 nm to 670 nm, with 1 nm

increments, with 8 channels AOTF for simultaneous control of 8 laser lines, each excitation wavelength provides 1.5

mV), and Ti-Sapphire laser (~4 W at 800 nm) which corresponded to approximately 1% (~40 mW at 800 nm) average

power in the focal plane. A PMT detector ranging from 420 nm to 700 nm for steady-state fluorescence was used with

Leica and images were processed with Leica Application Suite Advanced Fluorescence (LAS AF).

Human recombinant JNK1 (M33-10G-10) and its substrate ATF2 (A10-55G-20) were purchased from SignalChem.

JNK-IN-8 was a gift from Prof. Nathanael S. Gray (Harvard Medical School). Anisomycin (2222S), antibodies against

Phospho-JNK (9255S), total JNK (9252S) and Phospho-c-Jun (9261S) were obtained from Cell Signaling Technology.

Human JNK1 plasmid (transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag;

HG10795-ACG) was purchased from Sino Biological Inc. FITC-conjugated Goat anti-rabbit IgG (H+L) secondary

antibody (A16097), Lipofectamine® 2000 Transfection Reagent (11668027) and Opti-MEM® (Reduced Serum Medium;

31985070) were bought from Thermo Fisher Scientfic. Cell lines (HeLa, HEK293T, SH-SY5Y) were purchased from

ATCC.

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Table S1. Summary of probes and reporters used in the current study. *The following reporters were prepared based

on reported literature: TER-Tz1;[1] TMR-Tz;[2] Biotin-Tz2;[1] TER-N3;[3] Biotin-N3[4]

.

Scheme S1. Design of bioorthogonal small-molecule probes for JNKs (JP2-TCO/JP2-CP/JP3) based on the

irreversible inhibitor selective for JNKs (JNK-IN-8). With proper tags, they can either be clicked with (left)

conventional reporters containing rhodamine/biotin for in situ proteomic studies or (right) two-photon fluorogenic

reporters (QT1/QT2/AAN-N3) for no-wash live-cell imaging.

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2. Chemical Synthesis and Characterizations

Scheme S2. Synthesis of small-molecule probes and reporters for JNKs. (A) Synthesis scheme of covalent ABPs

derived from JNK-specific irreversible inhibitor JNK-IN-8. (B) Synthesis of various two-photon fluorogenic reporters

derived from acedan.

(J1) J1 was synthesized according to published procedures.[5] 3-(dimethylamino)-1-(pyridin-3-yl) prop-2-en-1-one (2.64

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g, 15.0 mmol, obtained according to a previously published procedure)[5] and guanidinium hydrochloride (1.50 g, 15.7

mmol) were mixed in 25 mL of 2-propanol. To the suspension was added NaOH (0.70 g, 17.5 mmol) and the mixture was

refluxed for 24 h. The reaction was then cooled to 0 oC and the precipitate was filtered off, suspended in water, filtered

off once more and washed with 2-propanol and diethyl ether. The residue was dried in oven at 60 to 70 oC and the desired

product was obtained as a white powder (1.876 g, 72.6%). 1H NMR (300 MHz, CDCl3) δ 9.20 (d, J = 2.2 Hz, 1H), 8.70

(dd, J = 4.7, 1.3 Hz, 1H), 8.39 (d, J = 5.2 Hz, 1H), 8.31 (dt, J = 8.0, 1.6 Hz, 1H), 7.41 (dd, J = 8.0, 4.8 Hz, 1H), 7.07 (d, J

= 5.2 Hz, 1H), 5.20 (s, 2H).

(J2)

J2 was synthesized according to published procedures.[5] J1 (2.4776 g, 14.39 mmol), CuI (0.6228 g, 3.27 mmol) and

anhydrous K2CO3 (3.6157 g, 26.16 mmol) were added to a dry RBF. The RBF was filled with N2 gas and subsequently

added a mixture of 2-bromo-5-nitrotoluene (2.8254 g, 13.08 mmol) and N,N'-dimethylethylenediamine (DMEDA, 0.35

mL, 3.25 mmol) in anhydrous dioxane (110 mL) at room temperature. The reaction mixture was stirred at 120 oC for 24 h,

before being cooled to room temperature. Subsequently, concentrated ammonia (55 mL) and brine (220 mL) were added

and the resulting mixture was extracted with EtOAc (5 × 220 mL). The organic layers were dried over Na2SO4,

concentrated under reduced pressure and the residue was purified by flash chromatography (DCM/MeOH = 50/1) to give

J2 as a yellow powder (1.60 g, 39.8%). 1H NMR (300 MHz, CDCl3) δ 9.29 (s, 1H), 8.76 (s, 1H), 8.71 (d, J = 9.1 Hz, 1H),

8.61 (d, J = 5.2 Hz, 1H), 8.43-8.27 (m, 1H), 8.19 (dd, J = 9.1, 2.7 Hz, 1H), 8.13 (d, J = 2.5 Hz, 1H), 7.48 (dd, J = 7.9, 4.8

Hz, 1H), 7.34 (t, J = 4.9 Hz, 2H), 2.48 (s, 3H).

(J3)

J3 was synthesized according to published procedures.[6] To a solution of J2 (1.60 g, 5.21 mmol) in 125 mL of

EtOAc and 25 mL MeOH was added SnCl2 (2.96 g, 15.61 mmol). The reaction was stirred for 24 h at 80 °C, and the

product was obtained as a yellow solid (1.07 g, 73.9%), which was directly used in subsequent steps without further

purification. 1H NMR (300 MHz, CDCl3) δ 9.22 (s, 1H), 8.68 (s, 1H), 8.41 (d, J = 5.2 Hz, 1H), 8.28 (dt, J = 8.0, 1.8 Hz,

1H), 7.40 (dd, J = 15.7, 7.8 Hz, 2H), 7.05 (d, J = 5.2 Hz, 1H), 6.87 (s, 1H), 6.66-6.45 (m, 2H), 3.27 (s, 3H), 2.22 (s, 3H). 13C NMR (75 MHz, CDCl3) δ 162.47, 161.76, 159.08, 151.19, 148.44, 143.91, 134.37, 133.50, 128.20, 126.13, 123.56,

123.49, 117.12, 113.20, 107.13, 18.19. ESI-MS: m/z [M+H]+ calcd, 278.13; found, 277.95.

(J4)

J4 was synthesized according to published procedures.[6] To a solution of J3 (0.195 g, 0.70 mmol) in pyridine (4 mL)

at 0 oC was added 3-nitrobenzyl chloride (0.158 g, 0.84 mmol) in DCM (2 mL). After being stirred for 12 h at 90 °C, the

reaction mixture was washed with CuSO4 (aq.) and extracted with EtOAc. The organic layers were dried over anhydrous

Na2SO4 and concentrated. The resulting crude product was directly used in the next step without further purification. 1H

NMR (300 MHz, CDCl3) δ 9.28 (s, 1H), 8.76 (s, 2H), 8.41 (ddd, J = 25.5, 20.9, 6.4 Hz, 5H), 8.06 (d, J = 8.7 Hz, 1H),

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7.69 (dd, J = 17.3, 9.3 Hz, 2H), 7.58-7.38 (m, 2H), 7.19 (d, J = 5.2 Hz, 1H), 7.06 (s, 1H), 2.38 (s, 3H). ESI-MS: m/z

[M+H]+ calcd, 427.14; found, 427.15.

(J5)

J5 was synthesized according to the published procedures.[6] J4 (0.2131 g, 0.50 mmol) was suspended in

EtOAc/MeOH (5/1, 18 mL in total) and treated with SnCl2 (0.2844 g, 1.50 mmol). Upon being stirred for 24 h at 80 °C,

the reaction mixture was cooled to room temperature and poured into saturated aqueous NaHCO3 (18 mL). The mixture

was stirred for 10 min and the aqueous phase was then extracted with chloroform and 2-propanol (v/v = 4/1). The

combined organic layers were dried over Na2SO4 and concentrated under reduced pressure. The resulting crude product

was purified by flash column chromatography with DCM/MeOH = 20/1 to provide the title compound as a yellow solid

(0.1121 g, 56.6% yield). 1H NMR (300 MHz, CDCl3) δ 9.17 (d, J = 1.9 Hz, 1H), 8.62 (dd, J = 4.8, 1.5 Hz, 1H), 8.39 (d, J

= 5.2 Hz, 2H), 8.26 (dt, J = 8.0, 1.8 Hz, 1H), 7.83 (d, J = 8.7 Hz, 1H), 7.53 (d, J = 2.1 Hz, 1H), 7.43 (dd, J = 8.6, 2.3 Hz,

1H), 7.34 (dd, J = 7.9, 4.8 Hz, 1H), 7.22-7.09 (m, 4H), 7.05 (d, J = 5.2 Hz, 1H), 6.73 (dt, J = 6.6, 2.2 Hz, 1H), 3.90 (s,

2H), 2.24 (s, 3H). 13C NMR (75 MHz, CDCl3) δ 166.18, 162.32, 160.84, 158.94, 151.15, 148.25, 146.96, 136.01, 134.42, 134.23, 133.57, 132.57, 130.58, 129.32, 123.56, 123.19, 122.59, 118.64, 117.99, 116.43, 113.74, 107.72, 18.11. ESI-MS:

m/z [M+H]+ calcd, 397.17; found, 397.10.

(C1)

C1 was synthesized according to published procedures.[7] A two-neck flame-dried 250 mL round bottomed flask

(RBF) was outfitted with a stir bar, gas inlet adapter and a rubber septum, then filled with nitrogen. The flask was

charged with rhodium tetraacetate (0.2210 g, 0.50 mmol) and trimethylsilyl acetylene (2.2450 g, 22 mmol) sequentially,

and while being stirred, the mixture was added ethyl diazoacetate (1.141 g, 10 mmol) at a rate of 0.5 mL/h via a syringe

pump. Upon completion of the addition, the resulting mixture was further stirred for 30 min before being concentrated in

vacuo and purified by flash chromatography (EtOAc/hexane = 1/30) to give 1.8400 g (92.8%) of the title compound as a

colorless oil. 1H NMR (300 MHz, CDCl3) δ 4.17-3.95 (m, 2H), 2.15 (s, 3H), 1.94 (s, 1H), 1.19 (t, J = 7.2 Hz, 3H), 0.15 (s,

9H). 13C NMR (75 MHz, CDCl3) δ 176.86, 122.57, 104.11, 59.66, 21.16, 14.34, 11.71, -1.68.

(C2)

To a stirred solution of C1 (1.8400 g, 9.3 mmol) in dry THF (80 mL) at -78 ºC was slowly added a solution of 1.0 M

DIBAL-H in THF (41 mL, 41 mmol) dropwise.[8] The reaction mixture was stirred for additional hour at -78 ºC before

being carefully quenched with a saturated potassium sodium tartrate aqueous solution. Upon solvent removal in vacuo,

the residue was extracted by EtOAc (4x). The organic layers were combined and washed with brine. The solvent was

removed and the product was purified by flash chromatography (hexane/EtOAc = 5/1) to afford 1.1180 g (76.9% yield)

of the desired product as a colorless oil. 1H NMR (300 MHz, CDCl3) δ 3.43 (ddd, J = 11.9, 4.6, 1.5 Hz, 2H), 2.17 (s, 3H),

1.61-1.43 (m, 1H), 0.81 (s, 1H), 0.13 (s, 9H). 13C NMR (75 MHz, CDCl3) δ 135.52, 111.31, 69.28, 22.08, 13.28, -1.24.

(C3)

C3 was synthesized according to published procedures.[9] C2 (30 mg, 0.19 mmol) was dissolved in THF (1 mL)

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under nitrogen, treated with TBAF in THF (1 M solution, 0.22 mL) at 0 oC and the solution was stirred at room

temperature for 2 h. Then it was cooled to 0 °C and pyridine (0.8 mL) and 4-nitrophenyl chloroformate (102 mg, 0.51

mmol) were added. The reaction mixture was stirred and allowed to warm to room temperature overnight. The reaction

mixture was concentrated and purified by flash chromatography (EtOAc/hexane = 1/8) to yield C3 as a colorless oil (20

mg, 42.2% yield). 1H NMR (300 MHz, CDCl3) δ 8.49-8.15 (m, 8H), 7.45-7.34 (m, 8H), 6.61 (s, 4H), 4.17 (ddd, J = 22.9,

10.7, 5.1 Hz, 9H), 2.18 (d, J = 1.0 Hz, 13H), 1.78 (td, J = 5.3, 1.5 Hz, 6H).

(A1)

A1 was synthesized according to published procedures.[10] To a stirring solution of NaH (50 mg, 2.08 mmol) in THF

(4 mL) at 0 oC was added triethylphosphonoacetate (0.374 mL, 1.88 mmol) dropwise. After being warmed up to ambient

temperature, a solution of N-Boc-2-aminoacetaldehyde (100 mg, 0.63 mmol) in THF (1 mL) was added. The reaction

mixture was stirred at ambient temperature until the reaction was completed as judged by TLC. Subsequently, the

mixture was diluted with water and extracted with EtOAc three times. The combined organic layers were dried over

Na2SO4, concentrated in vacuo, then purified by flash chromatography (EtOAc/hexane = 1/5) to give A1 (45 mg, 31.2%)

as a clear oil. 1H NMR (300 MHz, CDCl3) δ 6.86 (dt, J = 15.7, 4.9 Hz, 1H), 5.89 (dt, J = 15.7, 1.9 Hz, 1H), 4.87 (s, 1H),

4.15 (q, J = 7.1 Hz, 2H), 3.87 (s, 2H), 1.41 (s, 9H), 1.24 (t, J = 7.1 Hz, 3H). 13C NMR (75 MHz, CDCl3) δ 166.02, 155.55, 144.73, 121.21, 79.72, 60.31, 41.24, 28.24, 14.10.

(A2)

To a solution of A1 (45 mg, 0.20 mmol) in THF (1.25 mL) was added LiOH (19.2 mg, 0.80 mmol; dissolved in 0.83

mL of water) at 0 oC, and the resulting mixture was stirred at ambient temperature overnight. Upon removal of THF in

vacuo, the resulting yellow aqueous solution was acidified with HCl (1 M) to pH = 3. The organic layer was next

extracted with DCM three times, combined and dried over Na2SO4 before being concentrated in vacuo. Finally, the

mixture was purified by flash chromatography to give A2 (39.2 mg, 97.4%). 1H NMR (300 MHz, CDCl3) δ 6.97 (dd, J =

15.7, 4.3 Hz, 1H), 5.92 (d, J = 15.7 Hz, 1H), 4.84 (s, 1H), 3.93 (s, 2H), 1.44 (s, 9H). 13C NMR (75 MHz, CDCl3) δ 170.34, 147.07, 120.68, 79.97, 41.33, 29.64, 28.28.

(A3)

PCl5 (188.2 mg, 0.90 mmol) was added to a solution of A2 (181.9 mg, 0.90 mmol) in DCM (8 mL) at room

temperature, and the mixture was refluxed at 50 oC for 1.5 h. Upon being cooled down to room temperature, J5 (160.0

mg, 0.40 mmol) in DCM (2 mL) was added, and the mixture was refluxed for another 2 h. Purification by flash

chromatography (DCM/MeOH = 40/1) gave A3 (42.2 mg, 18.2%). 1H NMR (300 MHz, DMSO) δ 10.32 (s, 1H), 10.22 (s, 1H), 9.27 (s, 1H), 8.93 (s, 1H), 8.71 (s, 1H), 8.48 (d, J = 4.8 Hz, 1H), 8.39 (d, J = 7.8 Hz, 1H), 8.30 (s, 1H), 8.19 (s, 1H),

7.93 (d, J = 7.9 Hz, 1H), 7.75-7.58 (m, 3H), 7.47 (dd, J = 10.8, 8.6 Hz, 2H), 7.37 (d, J = 5.0 Hz, 1H), 7.20 (s, 1H), 6.79

(dd, J = 15.2, 4.4 Hz, 1H), 6.21 (d, J = 15.3 Hz, 1H), 3.79 (s, 2H), 2.26 (s, 3H), 1.41 (s, 9H). 13C NMR (75 MHz, DMSO)

δ 168.52, 166.95, 165.33, 163.46, 161.51, 161.40, 159.39, 155.54, 151.27, 148.04, 142.31, 139 .41, 135.87, 135.72,

134.15, 133.62, 132.81, 128.70, 125.57, 123.60, 122.24, 122.16, 118.85, 118.24, 107.28, 79.15, 77.94, 40.78, 28.23,

18.37. ESI-MS: m/z [M+H]+ calcd, 580.26; found, 580.25.

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(JP2-TCO)

To a solution of A3 (42.2 mg, 0.073 mmol) in DCM (0.4 mL) was added TFA (0.4 mL TFA in 1.2 mL DCM) at 0 oC.

The solution was stirred for 1 h at room temperature and the solvent was removed in vacuo. A portion of the resulting

residue (5.3 mg, 0.011 mmol) was dissolved in THF, followed by addition of DIEA (14 μL, 0.080 mmol) and TCO-NHS

ester (2.7 mg, 0.010 mmol) at 0 oC. The resulting mixture was stirred at room temperature overnight and purified by

column (EtOAc/DCM = 4/1) to give JP2-TCO as a yellow solid (5.3 mg, 83.9%). 1H NMR (300 MHz, CDCl3) δ 9.14 (d, J = 42.1 Hz, 2H), 8.87 (s, 1H), 8.67 (s, 1H), 8.41 (s, 1H), 8.28 (d, J = 7.4 Hz, 1H), 7.96 (s, 1H), 7.79 (d, J = 8.4 Hz, 2H),

7.58-7.30 (m, 4H), 7.24 (s, 2H), 7.10 (d, J = 4.2 Hz, 1H), 6.79 (d, J = 13.9 Hz, 1H), 6.06 (d, J = 14.9 Hz, 1H), 5.71-5.28

(m, 3H), 3.80 (s, 2H), 2.21 (s, 3H), 1.97-1.39 (m, 11H). 13C NMR (75 MHz, CDCl3) δ 165.96, 164.14, 162.46, 160.83, 158.87, 156.35, 151.11, 148.22, 142.00, 138.51, 135.57, 134.78, 134.65, 134.26, 133.68, 132.87, 130.71, 129.65, 129.49,

129.10, 124.06, 123.73, 123.30, 122.87, 119.05, 118.92, 107.88, 107.80, 103.78, 77.18, 41.71, 41.05, 38.54, 32.24, 29.63,

22.11, 18.17. ESI-HRMS: m/z [M+H]+ calcd, 632.2980; found, 632.2985.

(JP2-CP)

To a solution of A3 (42.2 mg, 0.073 mmol) in DCM (0.4 mL) was added TFA (0.4 mL TFA in 1.2 mL DCM) at 0 oC.

The solution was stirred for 1 h at room temperature and the solvent was removed in vacuo. A portion of the residue (19.2

mg, 0.040 mmol) was dissolved in THF (2 mL) followed by addition of DIEA (56 μL, 0.32 mmol) and C3 (9.0 mg, 0.036

mmol) at 0 oC. The mixture was stirred at room temperature overnight and purified by flash chromatography using

EtOAc/DCM = 4/1 to give JP2-CP as a yellow solid (10.6 mg, 50%). 1H NMR (300 MHz, DMSO) δ 10.31 (s, 1H), 10.21 (s, 1H), 9.25 (s, 1H), 8.94 (s, 1H), 8.70 (s, 1H), 8.49 (d, J = 4.9 Hz, 1H), 8.40 (d, J = 7.8 Hz, 1H), 8.17 (s, 1H),

7.91 (d, J = 8.0 Hz, 1H), 7.75 – 7.28 (m, 8H), 6.98-6.72 (m, 2H), 6.21 (d, J = 15.2 Hz, 1H), 3.84 (d, J = 3.6 Hz, 3H), 2.26

(s, 3H), 2.12 (s, 2H), 1.68-1.48 (m, 1H), 1.36-1.07 (m, 1H), 0.90 (t, J = 7.2 Hz, 1H). 13C NMR (75 MHz, DMSO) δ 165.68, 163.78, 161.87, 161.77, 159.80, 156.86, 151.71, 148.43, 142.48, 139.75, 136.23, 136.08, 134.55, 133.98, 133.21,

132.64, 129.10, 125.98, 124.18, 124.02, 122.59, 122.53, 120.69, 119.18, 118.59, 107.66, 102.64, 71.55, 65.84, 41.45,

18.73, 17.15, 11.77. ESI-MS: m/z [M+H]+ calcd, 590.24; found, 590.15. ESI-HRMS: m/z [M+Na]+ calcd, 612.2330;

found, 612.2336.

(B1)

Methyl trans-4-bromo-2-butenoate (1.79 g, 10 mmol) was dissolved in THF (40 mL). H2O (24 mL) was added

along with LiOH (0.79 g, 33 mmol) and the reaction was stirred for 3 h,[11] at which time complete consumption of the

starting material was confirmed by TLC (EtOAc/hexane = 1/4). Next, upon THF removal in vacuo, the remaining

aqueous solution was adjusted to pH = 3 by the addition of 1 M HCl (aq.). The product was extracted with DCM (3 × 10

mL) and the combined organic layers were dried over Na2SO4 and concentrated under reduced pressure to afford 1.3922

g (84.4%) of the desired product. 1H NMR (300 MHz, CDCl3) δ 9.76 (s, 1H), 7.11 (dt, J = 14.7, 7.3 Hz, 1H), 6.04 (d, J =

15.3 Hz, 1H), 4.03 (dd, J = 7.3, 0.5 Hz, 2H). 13C NMR (75 MHz, CDCl3) δ 170.57, 144.15, 123.80, 28.63.

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(B2)

PCl5 (20.8 mg, 0.10 mmol) was added to B1 (16.4 mg, 0.10 mmol; dissolved in 1 mL DCM) at room temperature.

Then the mixture was refluxed for 1.5 h. Upon being cooled down to room temperature, the mixture was added J5 (15.0

mg, 0.038 mmol; in 1 mL DCM), followed by refluxing for another 1.5 h. Finally, the mixture was quenched with

NaHCO3, extracted with EtOAc, and purified by flash chromatography with EtOAc gave B2 (15.0 mg, 72.6%). ESI-MS:

m/z [M+H]+ calcd, 543.11; found, [M+H]+ 543.09 and 545.08 (isotopes of Br).

(JP3)

N-Methylpropargylamine (5.0 μL, 0.060 mmol) in THF (0.5 mL) was added dropwise to a stirring solution of B2

(13 mg, 0.024 mmol; in anhydrous THF) at 0 oC. The resulting mixture was stirred at 50 oC for 60 h. following solvent

removal, the residue was purified by flash chromatography (DCM/MeOH from 50/1 to 20/1) to give JP3 as a yellow

solid (4.3 mg, 33.8 %). 1H NMR (300 MHz, DMSO) δ 10.37 (s, 1H), 10.21 (s, 1H), 9.24 (s, 1H), 8.92 (s, 1H), 8.69 (d, J

= 4.6 Hz, 1H), 8.48 (d, J = 5.0 Hz, 1H), 8.40 (d, J = 7.5 Hz, 1H), 8.18 (s, 1H), 7.91 (d, J = 7.8 Hz, 1H), 7.66 (s, 2H),

7.63-7.42 (m, 4H), 7.39 (d, J = 5.1 Hz, 1H), 6.85-6.64 (m, 1H), 6.35 (d, J = 15.2 Hz, 1H), 3.39 (s, 2H), 3.24 (s, 2H), 2.29

(s, 3H), 2.25 (s, 3H). 13C NMR (75 MHz, DMSO) δ 170.71, 165.66, 163.63, 161.84, 161.74, 159.83, 151.73, 148.43,

139.69, 136.20, 136.08, 134.56, 133.95, 133.22, 132.62, 129.11, 125.99, 124.21, 122.61, 122.56, 122.48, 119.16, 118.56,

107.65, 79.56, 60.11, 56.15, 45.19, 41.48, 18.76. ESI-HRMS: m/z [M+Na]+ calcd, 554.2275; found, 554.2289.

(AME-1)

AME-1 was synthesized according to the published procedure.[12] To a solution of

2-((6-bromonaphthalen-2-yl)(methyl)amino)ethanol (0.1440 g, 0.5 mmol[13]) in pyridine (3 mL) was added CuCN

(0.0840 g, 0.94 mmol) in a microwave tube and the mixture was stirred at 220 oC for 4 h. The mixture was cooled to

room temperature and the product was extracted with EtOAc, washed with 10% ethylenediamine (aq.) and brine, and

purified by flash column (EtOAc/hexane = ½) to give AME-1 (0.0650g, 57.4%). 1H NMR (500 MHz, CDCl3) δ 7.93 (s, 1H), 7.65 (d, J = 9.2 Hz, 1H), 7.58 (d, J = 8.6 Hz, 1H), 7.38 (d, J = 8.5 Hz, 1H), 7.29-7.18 (m, 1H), 6.87 (d, J = 2.2 Hz,

1H), 3.88 (t, J = 5.7 Hz, 2H), 3.64 (t, J = 5.7 Hz, 2H), 3.13 (s, 3H). 13C NMR (125 MHz, CDCl3) δ 149.65, 136.62, 133.61, 129.46, 126.76, 126.73, 125.03, 120.18, 116.98, 105.29, 103.90, 60.11, 54.63, 38.88. IT-TOF-MS: m/z [M+H]+

calcd, 227.11; found, 227.11.

(QT2)

QT2 was synthesized according to published procedures.[14] Hydrazine hydrate monohydrate (1.22 mL, 25 mmol)

was added dropwise to a mixture containing 1 (0.1200g, 0.53 mmol), Zn(OTf)2 (0.0910g, 0.25 mmol), molecular sieves

and acetonitrile (0.261 mL) in a microwave tube, and the tube was sealed and stirred for 24 h at 60 oC. Upon being

cooled down to room temperature, NaNO2 (1.22 g, 17.7 mmol) dissolved in water (10 mL) was added slowly, followed

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by 1 M HCl until pH = 3 and when the evolving gas ceased. The reaction mixture was extracted with DCM three times,

dried over anhydrous Na2SO4 then purified by flash chromatography (DCM/ EtOAc = 10/1) to give QT2 (0.0400 g,

25.6%). 1H NMR (300 MHz, CDCl3) δ 9.00 (s, 1H), 8.49 (dd, J = 8.7, 1.5 Hz, 1H), 7.88 (d, J = 9.1 Hz, 1H), 7.79 (d, J =

8.8 Hz, 1H), 7.25 (dd, J = 9.2, 2.4 Hz, 1H), 7.01 (s, 1H), 3.92 (t, J = 5.7 Hz, 2H), 3.67 (t, J = 5.6 Hz, 2H), 3.16 (s, 3H),

3.07 (s, 3H). 13C NMR (75 MHz, CDCl3) δ 166.47, 164.48, 149.48, 137.41, 130.74, 128.83, 127.11, 126.40, 124.35,

116.70, 105.94, 60.37, 55.01, 39.03, 21.04. ESI-HRMS: m/z [M+H]+ calcd, 296.1506; found, 296.1508.

(AMN-1)

To a solution of AMN (0.1838 g, 0.92 mmol) in CH3CN (5 mL) was added Na2HPO4·12 H2O (0.4954 g, 1.38 mmol),

BrCH2COOCH3 (131 μL, 1.38 mmol) and NaI (0.0553 g, 0.37 mmol). The resulting mixture was refluxed for 24 h,

before being cooled down to room temperature and purified by flash chromatography (EtOAc/DCM = 1/30) to give

AMN-1 (0.2441 g, 98%) as a yellow solid. 1H NMR (500 MHz, CDCl3) δ 8.28 (s, 1H), 7.90 (dd, J = 8.6, 1.5 Hz, 1H),

7.76 (d, J = 9.1 Hz, 1H), 7.61 (d, J = 8.7 Hz, 1H), 7.05 (dd, J = 9.1, 2.5 Hz, 1H), 6.85 (d, J = 2.3 Hz, 1H), 4.17 (s, 2H),

3.71 (s, 3H), 3.15 (s, 3H), 2.63 (s, 3H). 13C NMR (125 MHz, CDCl3) δ 197.54, 170.78, 148.62, 137.36, 131.07, 130.77,

130.09, 126.27, 125.41, 124.43, 115.57, 105.71, 53.94, 51.91, 39.44, 26.22. IT-TOF-MS: m/z [M+H]+ calcd, 272.12;

found, 272.10.

(AMN-2)

To a solution of AMN-1 (0.2441 g, 0.90 mmol) in THF (2.5 mL) was added EtOH (2.5 mL) and water (2.5 mL),

followed by LiOH·H2O (0.1887 g, 4.50 mmol). The resulting mixture was stirred at room temperature for 2.5 h before

being concentrated in vacuo. Upon addition of water (12.5 mL), the resulting aqueous layer was washed with DCM (5

mL, twice), then adjusted to pH = 3.0 with diluted HCl. After filtration, the crude product was taken and dried, washed

with DCM/hexane to give AMN-2 (0.2099 g, 91%) as a yellow solid. 1H NMR (500 MHz, D2O) δ 8.40 (s, 1H), 7.90-7.81

(m, 2H), 7.66 (d, J = 8.7 Hz, 1H), 7.20 (d, J = 9.1 Hz, 1H), 6.96 (d, J = 2.3 Hz, 1H), 4.28 (s, 2H), 3.19 (s, 3H), 2.65 (s,

3H). 13C NMR (125 MHz, MeOD) δ 200.49, 174.26, 150.82, 139.34, 139.27, 132.07, 127.56, 127.50, 126.98, 125.28, 117.21, 106.86, 54.73, 39.98, 26.51. IT-TOF-MS: m/z [M+H]+ calcd, 258.11; found, 258.09.

(QT1)

To a solution of AMN-2 (0.0029 g, 0.011 mmol) and HATU (0.0046 mg, 0.012 mmol) in DMF (0.4 mL) was added

DIEA (5.2 μL) and the reaction was stirred for 10 min at room temperature under argon atmosphere. NH2-tetrazine[15]

(0.0032 g, 0.01 mmol) was then added and the reaction was stirred for 6 h at room temperature. The reaction mixture was

diluted with EtOAc and washed with saturated NaHCO3 and brine. The organic layer was dried over anhydrous Na2SO4

and concentrated in vacuo. The crude product was purified by flash chromatography (EtOAc/hexane = 1/2) to give QT1

(0.0028g, 50.3%) as a pink solid. 1H NMR (300 MHz, CDCl3) δ 8.76 (d, J = 8.2 Hz, 2H), 8.54 (s, 1H), 8.50 (s, 1H), 8.44

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(d, J = 7.8 Hz, 1H), 8.38 (s, 1H), 8.14 (d, J = 9.4 Hz, 1H), 8.00 (dd, J = 8.6, 1.7 Hz, 1H), 7.92 (d, J = 9.1 Hz, 1H), 7.88 (d,

J = 8.3 Hz, 2H), 7.75 (d, J = 8.8 Hz, 1H), 7.62 (dd, J = 10.1, 5.8 Hz, 1H), 7.21 (d, J = 2.5 Hz, 1H), 7.13 (d, J = 2.3 Hz,

1H), 4.19 (s, 2H), 3.31 (s, 3H), 2.69 (s, 3H). ESI-HRMS: m/z [M+H]+ calcd, 557.1835; found, 557.1923.

(AAN-N3)

AAN-N3 was synthesized according to published procedures.[16] To an ice-cooled RBF loaded with AAN[17] (0.0500

g, 0.27 mmol) was added 4 M H2SO4 (1.5 mL, 6.07 mmol) slowly. An ice-cooled solution of NaNO2 (0.0223 g, 0.32

mmol) in water (0.7 mL) was next added dropwise in 5 min. The reaction was stirred for 30 min at 0 oC. Then a solution

of NaN3 (0.0881 g, 1.35 mmol) in water (2.1 mL) was added dropwise, and the mixture was vigorously stirred for an

additional 45 min at room temperature. Afterwards, EtOAc was added and the stirring continued vigorously for 5 min.

The organic layer was separated, washed by water, brine and dried over anhydrous Na2SO4. Upon solvent removal in

vacuo, the crude product was further purified by flash chromatography to give AAN-N3 (0.0569 g, 99.8%) as a yellow

solid. 1H NMR (300 MHz, CDCl3) δ 8.43 (s, 1H), 8.05 (dd, J = 8.7, 1.8 Hz, 1H), 7.96 (d, J = 8.8 Hz, 1H), 7.81 (d, J = 8.7

Hz, 1H), 7.48 (d, J = 2.2 Hz, 1H), 7.23 (dd, J = 8.8, 2.3 Hz, 1H), 2.72 (s, 3H). 13C NMR (125 MHz, CDCl3) δ 197.64,

140.32, 136.32, 134.07, 131.60, 130.03, 130.00, 127.42, 125.22, 119.75, 115.63, 26.61. IT-TOF-MS: m/z [M+H]+ calcd,

212.07; found, 212.06.

3. Experimental Methods

3.1. Photophysical properties of probes. All compounds were diluted in DMSO (if not specified) and the

measurements were performed at room temperature. UV-vis absorption and fluorescence spectra were measured at a

concentration of 10 μM in 1-cm standard quartz cells by using a Shimadzu UV-vis spectrophotometer and a Perkin Elmer

LS50 spectrofluorometer, respectively. The fluorescence quantum yield (Φ) was determined by using fluorescein aqueous

NaOH solution (pH = 13; Φ = 0.95) as the reference through Фs = Фr(ns2EsAr)/(nr

2ErAs), where the subscripts s and r refer

to the sample and reference compound. E is the integrated area under the emission spectrum. A is the absorbance of the

solution at the excitation wavelength and n is the refractive index of the solvent. The two-photon excited fluorescence

intensity was measured at 760 nm at 1 or 10 μM of the compound as indicated. The two-photon action cross section

(TPA cross section, δФ) was calculated with AAN as the reference (whose two-photon properties had previously been

reported[18]), by using the equation δsФs= δrФr(Ssφrcr)/(Srφscs). The intensity of the detected signals was denoted as S. φ is

the overall fluorescence collection efficiency of the experimental apparatus. The number density of the molecules in

solution was denoted as c. δr is the two-photon absorption cross section of the reference molecule.

3.2. IC50 values of probes.[19] Concentration-dependent experiments were performed to determine the in vitro

inhibition potency and the binding affinity of the probes towards the catalytic domain of JNK. The inhibition assay was

performed with Kinase-Glo® Plus Luminescent Kinase Assay (V3772, Promega) following the manufacture’s instructions. Recombinantly active JNK1 (M33-10G-10, SignalChem; 0.1 μg in 1 μL), 1 μL probe/wilde type inhibitor (JNK-IN-8), substrate (0.2 μg ATF2 diluted in 10 μL kinase buffer; A10-55G-20, SingalChem) and ATP (final

concentration: 10 μM; add 10 μL of 22 μM stock solution) were mixed in the kinase buffer (40 mM Tris, pH 7.5, 20 mM

MgCl2, 0.1 mg/mL BSA) at a total volume of 22 µL in a 1.5 mL Eppendorf tube. The incubation was allowed to continue

for 20 min at 37 °C with shaking (700 rpm) and the reaction was subsequently quenched by addition of an equal volume

(22 µL) of the Kinase-Glo reagent. After ~5 min of incubation, 35 μL/well of the reaction mixture was transfered to a

flat-bottom solid white 384-well plate in duplicate, then the luminescence readouts from the wells were measured using a

BioTek Synergy 4 plate reader. The IC50 values of the probes were calculated from the percentage activity vs. log

[concentration of probe] curves generated from GraphPad Prism software.

3.3. In vitro labeling of probes towards recombinant JNK1. For gel-based in vitro protein labeling experiments,

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procedures were based on previously published protocols with some modifications.[19b, 20] 50 ng of recombinant JNK1

(M33-10G-10, SingalChem) was diluted in 20 μL HEPES (25 mM HEPES, 2 mM MgCl2, 150 mM NaCl, pH = 7.5)

containing 0.01% triton. JNK1 was incubated with 1 μM of probe for 1 h at room temperature with gentle shaking (600

rpm), followed by CuAAC/click chemistry (a mixture of 200 μM CuSO4, 20 μM THPTA, 200 μM TCEP and 10 μM

TER-N3 for JP3, or 10 μM TER-Tz1 for JP2-TCO and JP2-CP) for 2 h at room temperature. Next, 4 μL 6 × loading dye

without DTT was added into the mixture and, without boiling, all samples were resolved on a 12% SDS-PAGE gel. For

competition experiments, JNK1 was pretreated with 10 μM of JNK-IN-8 for 1 h at room temperature with gentle shaking

(600 rpm) before addition of probes. For AfBPs (i.e., SP-1 and IM-1), the reaction mixture in Eppendorf tubes was UV

irradiated (365 nm for 10 min at 4 °C) before click chemistry. In-gel fluorescence scanning (FL) was used to visualize the

labeled protein bands followed by silver staining (SS).

3.4. Cellular activities and in situ proteome profiling in mammalian Cells. Human carcinoma epithelial carcinoma

(HeLa), human embryonic kidney (HEK293T) and neuroblastoma (SH-SY5Y) cell lines were cultured in Dulbecco’s Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100.0 mg/L streptomycin and

100 IU/mL penicillin in a humidified atmosphere of 5% CO2 at 37 oC. For Western blotting (WB), equal amount of

lysates diluted in SDS loading buffer was separated by 12% SDS-PAGE before being transferred onto a Polyvinylidene

Fluoride Transfer Membrane (BioTraceTM PVDF). The PVDF membrane was then blocked with 3% BSA in 1× TBS

containing 0.1% Tween-20 (TBST) for 1 h at room temperature. Then the membrane was probed with a suitable primary

antibody (1:1000) and shaken at room temperature for 1 h or at 4 °C overnight. HRP-conjugated secondary antibody

(1:5000) was incubated at room temperature for 1 h. The antibody-reactive bands were visualized by chemiluminescent

detection (SuperSignal™ West Dura Chemiluminescent Substrate from Thermo Scientific™).

The cell-based kinase assays for c-Jun phosphorylation were carried out by using HeLa cells.[6] The phosphorylation

level of c-Jun was determined by Western blotting (WB) using the anti-phospho-c-Jun antibody (anti-p-c-Jun). Cells

were grown in 12-well plates to ~70% confluency. 0.5 mL of fresh DMEM (without FBS) was applied to the cells before

addition of compounds for 2 h. Then cells were treated with compounds at indicated concentrations for 3 h (compounds

were applied from DMSO stocks whereby DMSO never exceeded 1% in the final solution) followed by 1 h of

stimulation with 2 μM of anisomycin. The cells were collected by scraping and washed with PBS before being lysed with

100 μL 1 × SDS loading buffer. The sample was heated at 95 oC for 10 min and loaded at 10 μL/lane for WB analysis using anti-phospho-c-Jun antibody.

Transfection of JNK1-GFP plasmids was carried out according to the manufacturer’s guidelines (Lipofectamine®

2000 Transfection Reagent). One day before transfection, HeLa cells were seeded in a 24-well plate (for in situ

labeling)/4-well imaging dish (Greiner Bio-One, #627870; for cellular imaging) with 0.5 mL/well of DMEM (containing

10% FBS but without antibiotics) and left for 24 h until they reached ~80% confluency. The next day before transfection,

the old medium was replaced with 0.4 mL/well fresh DMEM (containing 10% FBS but without antibiotics). To prepare

plasmid-Lipofectamine® 2000 complexes: 1) dilute plasmids (0.8 µg/well for in situ labeling; 0.4 µg/well for cellular

imaging) in 50 µL of Opti-MEM® (Reduced Serum Medium) and mix gently; 2) dilute the appropriate amount of

Lipofectamine™ 2000 (2 µL/well for in situ labeling; 1 µL/well for cellular imaging) in 50 µL of Opti-MEM® and mix

gently before incubation for 5 min at room temperature. Afterwards, the diluted Lipofectamine® 2000 and diluted

plasmids were combined and incubated for 20 min at room temperature. The final 100 µL of plasmid-Lipofectamine®

2000 complexes were added to each well containing cells and medium. With gentle rocking of the plate, cells were

incubated at 37 °C in a CO2 incubator for ~20 h before further experiments.

For in situ proteome labeling, two methods were used: (1) for JNK1-GFP-transfected HeLa cells, transfected cells

(as described above in 24-well plates) were pretreated with/without WT (JNK-IN-8, 10 μM) in 250 μL of FBS-free

DMEM for 1 h before addition of probes at indicated concentrations for another 1 h. Cells were washed with PBS gently

before being detached by addition of 50 μL lysis buffer (HEPES buffer containing 0.1% triton and supplemented with

S1_14

protease inhibitor cocktail (04693159001 ROCHE)) and scraping. After being homogenized by sonication, the

supernatant was obtained (13000 rpm × 20 min at 4 °C). For AfBPs, cells were UV-irradiated (365 nm) for 15 min at

4 °C in PBS after probe addition. A freshly premixed click chemistry reaction cocktail (1 mM CuSO4, 0.1 mM THPTA, 1

mM TCEP and 25 μM TER-N3 for JP3/SP-1) or TER-Tz1 (10 μM for JP2-TCO/JP2-CP) was added to the supernatant.

The reaction was further incubated for 2 h with gentle mixing at room temperature, before being terminated by addition

of five-fold pre-chilled acetone (2 h at -20 °C). Precipitated proteins were subsequently collected by centrifugation

(13000 rpm × 15 min at 4 °C). The supernatant was discarded and the pellet was washed with MeOH/CHCl3/H2O (=

4/1/4). The residue was redissolved in 10 μL 2 × loading buffer and heated for 10 min at 95 °C. All proteins were loaded

and separated by 12% SDS-PAGE gel and then visualized by in-gel fluorescence scanning. WB against JNK and loading

control GAPDH were always carried out to indicate the transfection efficiency and total protein amount; (2) for in situ

labeling against endogenous JNKs, HEK293T, HeLa or SH-SY5Y cells were grown to ~80% confluency in 6-well (for

JP2-TCO) or 12-well (for JP3) plates. The medium was removed, and cells were washed with FBS-free DMEM and

incubated in 1 mL of FBS-free DMEM. Cells were then pretreated with/without WT (JNK-IN-8, 10 μM) for 1 h before addition of probes (JP2-TCO/JP3 at various concentrations) for another 1 h. Subsequently, cells were induced with or

without 2 μM of anisomycin for another 0.5 h. The cells were detached and washed gently with PBS twice to remove

excessive probes. The cells were resuspended in 200 μL (for 6-well plates) or 100 μL (for 12-well plates) of lysis buffer

(HEPES buffer containing 0.1% triton and supplemented with protease inhibitor cocktail (04693159001 ROCHE)), then

homogenized by sonication. The supernatant was clicked and treated as described in (1), followed by in-gel fluorescence

scanning. Coomassie Brilliant Blue (CBB) staining was always carried out afterwards.

3.5. Cellular imaging. For model studies with homopropargylglycine (HPG)[21] and AAN-N3, in-solution click

reactions were first carried out to determine the optimal condition suitable for cellular imaging with AAN-N3. The

following three click conditions were compared in PBS buffer or HeLa lysates (2 mg/mL) with AAN-N3 (0.1 mM) and

propargyl alcohol (0.2 mM), and the reactions were monitored by HPLC: Cond 1. 50 μM CuSO4, 300 μM THPTA, 2.5 mM NaASc; Cond 2. 1 mM CuSO4, 0.1 mM THPTA, 2 mM NaASC; Cond 3. 1 mM CuSO4, 300 μM THPTA, 10 mM NaASc. Each reaction was prepared by first adding CuSO4 (5 or 50 mM stock solution in H2O) and THPTA (5 mM stock

solution in H2O) to PBS. Next, freshly prepared NaASc (100 mM in PBS) was added. Finally, 5 mM of AAN-N3 in

DMSO and 8 mM of propargyl alcohol were added to a final volume of 200 μL. For click reaction in HeLa lysates, the

lysates (2 mg/mL) was added and mixed properly before addition of the alkyne. None of the above conditions however

provided strong signal-to-background contrast in cellular imaging with HPG-treated cells (data not shown).

For cellular imaging with JP3 in HeLa cells transfected with JNK1-GFP, transfection was first carried out as above

described. Upon washing with FBS-free DMEM and re-introduction of FBS-free DMEM (0.5 mL/well), cells were

treated with or without WT (JNK-IN-8, 20 μM) for 1 h before introduction of JP3 (5 μM) for an additional 1 h. For

comparison with AfBPs, probe-treated cells were UV-irradiated (365 nm) for 15 min at 4 °C in PBS, washed (with PBS)

and fixed with 3.7% formaldehyde in PBS at room temperature for 15 min. The formaldehyde solution was removed and

the cells were washed twice with PBS. The cells were then permeabilized with 300 μL 0.1% Triton-X-containing PBS for

10 min at room temperature. The Triton-X was removed and the cells were washed twice with PBS, then covered with

200 μL of click solution. The click solution comprised of 1 mM CuSO4, 100 μM THPTA, 1 mM TCEP, and 25 μM of TER-N3 (1 mM CuSO4 from 1 M stock solution in H2O was mixed with 0.1 mM THPTA from 0.1 M stock solution in

H2O and vortexed. 1 mM TCEP from 0.5 M stock solution in H2O was then added and vortexed. Finally, 25 μM TER-N3

(from 10 mM stock solution in DMSO) was added) and the solution was diluted with PBS before being applied to cells.

After 2-h incubation at room temperature in dark with gentle shaking, cells were washed with PBS at least three times

and 0.1% Triton-X in PBS for once, followed by incubation with anti-JNK (1:100, CST #9252S) at 4 °C overnight. Upon

washing with PBS for three times (10 min each) and incubation with FITC-conjugated Goat anti-rabbit IgG (H+L)

secondary antibody (1:200, ab6717) for 2 h at room temperature, cells were then washed with PBS for three times (10

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min each), 0.1% Triton-X-containing PBS for 10 min, PBS once again, and imaged.

For no-wash direct imaging with live mammalian cells, the followings were done: (1) Model studies by using a

TCO-containing puromycin probe (5T):[2] HeLa cells were grown to ~70% confluency in 4-well imaging dishes before

being treated with 10 μM of 5T in 0.5 mL DMEM for 2 h. Afterwards, cells were washed with FBS-free DMEM for

three times before reintroduction of 0.5 mL FBS-free DMEM containing 1 μM of QT1 or QT2 for another 1 h. Live-cell

imaging was directly carried out without washing; (2) Using JP2-TCO in JNK1-GFP-transfected HeLa cells. HeLa cells

were transfected with JNK1-GFP as described above before incubation with the probes. Upon washing with FBS-free

DMEM and re-introduction of FBS-free DMEM (0.5 mL/well), cells were treated with or without WT (JNK-IN-8, 4

times of probe) for 1 h before introduction of JP2-TCO (0.5 or 5 μM as indicated) for an additional 1 h. The cells were

then washed twice with FBS-free DMEM. 10 μM of QT1/QT2 from 1 mM stock solution was added to cells containing

0.5 mL fresh FBS-free DMEM and reacted for 1 h at 37 oC. Live-cell imaging was carried out directly without washing.

As a control, TMR-Tz (1 μM) was used instead of QT1/QT2 to check the cellular distribution of JP2-TCO. To remove

the background completely, after 1-h incubation, cells were washed with PBS for twice and treated with cold MeOH at

-20 oC for 5 min to fix and permeabilize the cells. Immunofluorescence (IF) imaging was carried out as described above

by using FITC-Rb for colocalization study of the fixed cells.

Microscopic light source and filters used for imaging include: (1) GFP/FITC, ex = 488 nm, em = 500-550 nm; (2)

TER-N3/TMR-Tz, ex = 557 nm, em = 570-650 nm; (3) QT1/QT2, ex = 405 nm and em = 420-550 nm for one-photon

channel, and ex = 760 nm, em = 450-550 nm for two-photon channel.

3.6. In situ pull-down (PD), Western blotting (WB) and LC-MS/MS experiments. To validate potential cellular targets

of the probes, pull-down (PD) experiments were carried out, followed by Western blotting. The general PD/WB

procedure was based on previously reported protocols,[19b, 20] with the following optimizations. Briefly, cells were grown

to ~80% confluency in 10-cm cell culture dishes containing 10 mL of fresh DMEM (without FBS). The probe (0.1 or 0.5

µM as indicated), with or without pretreatment of WT (10x, 1-h pre-incubation) for competition, was directly added to

live cells, followed by incubation at 37 ºC/5% CO2 for 1 h. DMSO should never exceed 1% in the final solution. 2 μM of anisomycin was used to activate JNKs where indicated. Cells treated with DMSO instead of probes served as a negative

control. The medium was subsequently removed by aspiration, and cells were washed gently with PBS twice to remove

excessive probe. The cells were then scraped and lysed using 2 mL of lysis buffer (HEPES buffer containing 0.1% triton

and supplemented with 0.1 mM of PMSF), followed by homogenization by sonication. The supernatant was collected by

centrifugation (13000 rpm × 20 min at 4 °C) and subjected to click reaction with 25 μM of Biotin-N3 (1 mM CuSO4, 0.1

mM THPTA, 1 mM TCEP, room temperature) for alkyne-containing probes, or 25 μM of Biotin-Tz2 (37 oC) for

TCO-/CP-containing probes for 2 h. After acetone precipitation (10 mL, -20 °C, overnight) and 5 mL-methanol wash (3x,

-80 °C, 1 h/each time), the air-dried pellets were resolubilized in 0.8 mL of 5% SDS-containing TBS with brief

sonication. 50 μL of the solution was kept as “before PD sample (BP)”. Additional 3 mL TBS was added to the remaining

solution, making the final concentration of SDS as 1%. Upon thorough mixing, these resuspended samples were then

centrifuged at 13000 rpm for 20 min. The collected supernatant was incubated with NeutrAvidin™ Agarose Resins (#

29204, Thermo Scientific; 100 µL/mg protein) for 5 h at room temperature with gentle shaking. After centrifugation

(1000 rpm, 1 min), the supernatant was removed and the beads were washed with 1% SDS in TBS twice, 5% SDS in

TBS once, 1% SDS in TBS twice and TBS without SDS once. After washing, the beads were boiled in 200 μL of 2 × SDS loading buffer for 30 min and then concentrated to 100 μL. Control samples treated with DMSO instead of probes were carried out concurrently. Results were analyzed by WB analysis.

For standard LC-MS/MS, the PD samples were first separated by 12% SDS-PAGE gel and subjected to CBB

staining. The avidin band (~15 kDa) was discarded and the remaining gel was excised and subjected to tryptic in-gel

digestion. Tryptic peptides were extracted from the gel and analyzed as previous described.[2, 13, 19b, 20, 22] All peptide

samples were dried in a speedvac and reconstituted with 20 L of 0.1% TFA/H2O. The peptides were separated and

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analyzed on a nanoAcquity (Waters) LC system coupled with Q-Exactive Orbitrap mass spectrometer (Thermo Scientific

Inc.). Samples were loaded onto a fused silica desalting column packed with 2 cm C18 reverse phase resin (Phenomenex

Inc.), and peptides were separated on a reverse phase column (100 μm fused silica packed with 25 cm C18 resin). Peptide

separation condition was a gradient 5-50% Buffer B in Buffer A (Buffer A: water, 0.1 % formic acid; Buffer B:

acetonitrile, 0.1% formic acid), and the flow rate through the column was set to 250 nL/min, and spray voltage was set to

1.6 kV. One full MS scan (350-1800 MW) was followed by 10 data dependent scans of most intense ions with dynamic

exclusion enabled. The generated tandem MS data was searched using IPI Human database (version: 3.87) using

Proteome Discoverer software (v.1.4.0.288). A maximum of three missed cleavages were allowed and fully tryptic

peptides were considered with dynamic modification of acetyl at N-terminal. The maximal mass tolerance in MS mode

was set to 10 ppm, and fragment MS/MS tolerance was set to 0.8 Da for HCD data. The maximum peptide and site false

discovery rates were specified as 0.01 using Percolator. The obtained peptide/protein list for each fraction was exported

to Microsoft Excel or processed using an in-house script for further analysis.

With standard LC-MS/MS data, DMSO-treated cells served as controls to distinguish JNK probe-enriched proteins

from non-specific proteins where putative hits from JP3/JP2-TCO/JP2-CP labeled samples were defined as those

proteins exclusively present in probe-treated cells but not in the DMSO control.[23]

S1_17

4. Results and Discussion

Fig. S1. Summary of performance of the probes and reporters. Cellular EC50 was determined from inhibition of endogenous c-Jun phosphorylation.[17] All measurements

of TP reporters were taken at 10 μM in DMSO at room temperature. [a]Peak position of the longest absorption/emission band (nm). [b]Brightness upon the absorption

maximum of the compound. [c]Two-photon action cross section values calculated at excitation wavelength of 760 nm in GM (1 GM = 10-50•cm4•s•photon-1). [d]The ratio of

brightness () from “reporter + TCO/Alkyne” over “reporter only”. NA = not available; ND = not determined; *reported results[19a].

S1_18

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 min

0.0

0.5

1.0

1.5

mAU(x100)254nm,4nm (1.00)

250 500 750 1000 1250 m/z0.0

2.5

5.0

7.5

Inten.(x100,000)

267.132

330.132655.188

250 500 750 1000 1250 m/z0.00

0.25

0.50

0.75

1.00

1.25

Inten.(x100,000)

655.197

328.945157.012

267.140

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 min

0.0

1.0

2.0

3.0

mAU(x100)254nm,4nm (1.00)

1

2

QT1

1

2

QT1+TCO

(A)

0.0 2.5 5.0 7.5 10.0 12.5 min

0.00

0.25

0.50

0.75

1.00

1.25

1.50

1.75

mAU(x1,000)Detector A Ch2:254nm

0.0 2.5 5.0 7.5 10.0 12.5 min

0.0

0.5

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

mAU(x100)Detector A Ch2:254nm

250 500 750 1000 1250 1500 1750 m/z0.0

2.5

5.0

7.5

Inten.(x100,000)

394.000

151.700

806.600430.000 577.300 1399.550294.850 1139.7501017.000

250 500 750 1000 1250 1500 1750 m/z0.0

1.0

2.0

Inten.(x100,000)

392.050

156.750

783.150294.000 520.100 1207.400995.100 1324.500656.950

1

2

1

2

(B)

QT2

QT2+TCO

Fig. S2. LC-MS characterization of QT1/QT2 + trans-cyclooctenol (TCO) ligation. (A) LC-MS characterizations of

the QT1 + TCO ligation reaction. 50 μL of QT1 (0.2 mM) was mixed with 50 μL of trans-cyclooctenol (TCO, 2 mM) in

DMSO and shaken for 0.5 h at 37 oC, followed by LC-MS analysis. (B) LC-MS characterizations of the QT2 + TCO

S1_19

ligation reaction. 50 μL of QT2 (0.2 mM) was mixed with 50 μL of TCO (2 mM) in DMSO and shaken for 0.5 h at 37 oC,

followed by LC-MS analysis.

100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 m/z0.0

0.5

1.0

1.5

2.0

2.5Inten.(x100,000)

268.084

157.019

382.1460

100

200

300

400

mAU254nm,4nm (1.00)

0

100

200

300

400

mAU254nm,4nm (1.00)(A) (C)

0

250

500

750

1000

1250mAU

254nm,4nm (1.00)

0

250

500

750mAU

254nm,4nm (1.00)

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min

0

1000

2000

3000

4000

mAU254nm,4nm (1.00)

t = 15 min

Cond.1

Cond.2

Cond.3

0

250

500

750

1000

1250mAU

254nm,4nm (1.00)

AAN-N3

0

250

500

750

1000

1250mAU

254nm,4nm (1.00)

0

250

500

mAU254nm,4nm (1.00)

t = 2 h

0

250

500

750

1000

1250

mAU254nm,4nm (1.00)

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min

0

250

500

750

1000

1250mAU

254nm,4nm (1.00)

AAN-N3

Cond.1

Cond.2

Cond.3

0

1000

2000

3000

mAU254nm,4nm (1.00)

0

250

500

750

1000

1250

mAU254nm,4nm (1.00)

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min

0

500

1000

1500

2000

2500

mAU254nm,4nm (1.00)

-250

0

250

500

750

1000

1250

1500

mAU254nm,4nm (1.00)

0

100

200

300

400

mAU254nm,4nm (1.00)

0

250

500

mAU254nm,4nm (1.00)

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 14.0 min

0

500

1000

1500

2000

mAU254nm,4nm (1.00)

Cond.1

Cond.2

Cond.3

AAN-N3 AAN-N3

Cond.1

Cond.2

Cond.3

0

250

500

750

1000

1250

mAU254nm,4nm (1.00)

0 min

15 min

2 h

1 2 3 4

1 2 3 4

1 2 3 4

PBS

2 h

0 min

15 min

1 2 3 4

1 2 3 4

1 2 3 4

HeLa lysates

PB

SH

eLa

lysa

tes

(B)

Fig. S3. Characterization of AAN-N3 + propargyl alcohol ligation. (A) LC-MS characterizations of the AAN-N3 +

Alkyne (propargyl alcohol) ligation reaction. 2 μL of CuSO4 (5 mM in H2O) was mixed with 6 μL of THPTA (10 mM in H2O) and vortexed before being added to 187 μL of water. Then 2 μL of AAN-N3 (10 mM in DMSO) and 2 μL of propargyl alcohol (20 mM in DMSO) were added and vortexed. Finally, 1 μL of sodium ascorbate (NaASc, 0.5 M in H2O)

was added and the solution was shaken for 2 h at room temperature, followed by LC-MS analysis. (B-C) In-solution click

reactions of AAN-N3 (0.1 mM) with propargyl alcohol (0.2 mM) under three different conditions: Cond. 1. 50 μM CuSO4, 300 μM THPTA, 2.5 mM NaASc; Cond. 2. 1 mM CuSO4, 0.1 mM THPTA, 2 mM NaASc; Cond. 3. 1 mM

CuSO4, 300 μM THPTA, 10 mM NaASc, were tested in PBS and HeLa lysates (2 mg/mL), respectively. Both (B) HPLC

analysis and (C) photos of the click solutions (under 365 nm excitation) were recorded at different time points of the

click reaction. Four tubes from left to right in (C): 1-Cond.1, 2-Cond.2, 3-Cond.3, 4-AAN-N3 only.

S1_20

Table S2. Photophysical properties of fluorogenic reporters (QT1, QT2 and AAN-N3) before and after click

reaction (complete conversion as determined by LC-MS). Parts of the results were reproduced in Fig. S1 in the SI.

Fig. S4. Photophysical properties of two-photon reporters before and after click reaction (prepared as in Fig. S2-3).

(A) One-photon excited fluorescence spectra of compounds (10 μM) in DMSO excited at 360 nm in 1-cm standard

quartz cells. (B) Representative two-photon excited fluorescence spectra of 10 μM compounds (except 1 μM for QT1

and QT1+TCO) excited at 760 nm.

S1_21

JNK-IN-8

0 2 4 6 80

50

100

150

IC50 = 37.47 ± 0.1 nM

Log(conc/pM)

% A

ctivity (

JN

K1)

JP2-CP

0 2 4 6 8 10

0

50

100

150

IC50 = 245.2 ± 0.1 nM

Log(conc/pM)

% A

ctivity (

JN

K1)

JP2-TCO

0 2 4 6 8 100

50

100

150

IC50 = 301.4 ± 0.1 nM

Log(conc/pM)

% A

ctivity (

JN

K1)

JP3

0 2 4 6 8 100

50

100

150

IC50 = 84.96 ± 0.1 nM

Log(conc/pM)

% A

ctivity (

JN

K1) Compound

JNK1

IC50 (nM)

JNK-IN-8 37.5 ± 0.1

JP3 85.0 ± 0.1

JP2-TCO 301.4 ± 0.1

JP2-CP 245.2 ± 0.1

SP600125* 48.8 ± 0.4

SP-1* 118.3 ± 0.2

0 0.1 0.2 0.5 1 2 0 0.1 0.2 0.5 1 2 5 μMJNK-IN-8 JP3

anti-p-c-Jun

anti-β-tubulin

JP2-TCO JP2-CP

0 0.1 0.2 0.5 1 2 5 0 0.1 0.2 0.5 1 2 5 μM

(C)

(A) (B)

Fig. S5. Bioactivity of parental inhibitors and probes derived from them. (A) IC50 plots of JP2-TCO/-CP, JP3 as

well as the wild-type inhibitor (JNK-IN-8), against recombinant JNK1. (B) Summary of IC50 values of JNK1 inhibition

by JNK-IN-8 (WT)/JP3/JP2-TCO/JP2-CP/SP600125/SP-1. *Reported IC50 values.[19a] (C) WB analysis of inhibition on

cellular phosphorylation of c-Jun (downstream substrate of JNK) by JNK probes or JNK-IN-8 at different concentrations

for 3 h, following anisomycin stimulation (2 μM, 1 h) in HeLa cells (serum-starved for 2 h before addition of

compounds). Results were summarized in Fig. S1 in the SI.

- + - + - + - + - + - + - + - + - + - +

anti-JNK

anti-GAPDH

7295

55

43

43

JP2-TCO JP3

WT (10 μM)

kDa

FL

72

55

43

34

95

Fig. S6. Concentration-dependent in situ reactivity profiles of JP3/JP2-TCO in JNK1-GFP-transfected HeLa cells.

Upon probe labeling, cells were lysed prior to click chemistry, SDS-PAGE separation and in-gel fluorescence scanning

(FL), WB with anti-JNK and anti-GAPDH (loading control). Where applicable, cells were pre-treated with WT (10 μM,

1 h) followed by probe treatment (0.001-1 μM, 1 h). (Arrowed): probe-labeled JNK1-GFP.

S1_24

Fig. S7. Cellular imaging using small-molecule probes/two-photon reporters for JNKs. (A) No-wash imaging of newly synthesized proteins labeled by 5T (10 μM, 2 h; structure shown on the right) in live HeLa cells with QT1/QT2 (1 μM, 1 h) under one- (ex = 405 nm, panels 1/4/7/10) or two-photon (ex = 760 nm, panels 2/5/8/11)

excitation, with em = 450-550 nm. (B-E) Cellular imaging of JNK1-GFP-transfected HeLa cells by using JP3/JP2-TCO. (B-C) Cellular localization of JP3/JP2-TCO upon

click reaction with TER-N3/TMR-Tz. Where applicable, 4-fold WT (JNK-IN-8) was used as a competitor to identify the specificity of fluorescent signals. Cells were fixed

and permeabilized, followed by immunofluorescence (FITC-labeled antibody) against JNK (the fluorescence from the expressed GFP was quenched after fixation). (D-E)

Live-cell imaging of JNK1-GFP-transfected HeLa cells with JP2-TCO (5 μM, 1 h) with or without WT (JNK-IN-8, 20 μM, 1-h pre-treatment). After a single wash to remove

unreacted probes, the cells were clicked with QT1/QT2 (10 μM, 1 h) followed by direct live-cell imaging. λex = 488 nm, λem = 500-550 nm for JNK1-GFP; λex = 557 nm, λem

= 570-650 nm for TER/TMR; λex = 405 nm, λem = 450-550 nm for QT1 and QT2. (F) Cellular imaging of AfBPs in JNK1-GFP-transfected HeLa cells in comparison with

JP3. Cellular localization of JP3/AfBPs was visualized upon click reaction with TER-N3. Where indicated, 4-fold WT (JNK-IN-8) was used as a competitor to identify the

specificity of fluorescent signals. JNK1-GFP transfected HeLa cells were treated with 5 μM of probes for 1 h with or without pre-treatment of WT (JNK-IN-8, 20 μM, 1 h). After a single wash to remove unreacted probes, the cells were UV irradiated (15 min on ice) in PBS and fixed prior to click chemistry. Immunofluorescence (FITC-labeled

antibody) against JNK was also conducted before imaging. λex = 488 nm, λem = 500-550 nm for JNK1-GFP; λex = 557 nm, λem = 570-650 nm for TER. Parts of the figure was

reproduced in Fig. 2 in the maintext. Scale bar = 20 μm.

S1_25

- + - + - + - + - + - + - + - + - + - + - + - + - + - + - + - + - + - +

HEK293T HeLa

WT (10 μM)

kD

a

7255

43

34

26

180

SH-SY5Y

26

180

FL

CB

B

- - + - - + - - +

HeLa HEK293T SH-SY5Y

7255

43

34

95

180

WT (10 μM)JP2-TCO (μM)

34

180

kD

a

FL

CB

B

- - + - + -

BP

P

D

An

ti-JNK

SH-SY5Y

JP3 JP2-TCO

(B)

(C) (D)

72

55

43

72

55

43

kD

a

95

- - + - - - + -

- - - + - - - +

Probe (μM)WT (10 x)

anisomycin

HEK293T

0 0.1 0.1 0.1 0 0.1 0.1 0.1

JP2-TCO JP3

0 0.5 0.5 0.5 0.5 0

+ + + + + +

0 1 1 0 1 1 0 1 1

anti-

GA

PD

H34

anti-JN

K

55

43

34

kDa

TPM HEK293 HeLa SH-SY5Y

JNK1 16.2 7.3 43.1

JNK2 34.0 31.7 31.8

JNK3 0.5 0.0 8.9

(A)

HEK293T HeLa SH-SY5Y0

100WB

WB

(tota

l JN

K)

TP

M (

JN

K1/2

/3)

JNK1

JNK2

JNK3

TPM

0

4

total JNK

JP3

Fig. S8. In situ profiling of endogenous JNKs in HeLa/HEK293T/SH-SY5Y cells upon labeling by JP3/JP2-TCO.

(A) The expression level of endogenous JNKs in HEK293T/HeLa/SH-SY5Y cells. (Left) RNA expression level (TPM:

Transcripts Per Kilobase Million) of JNK1/2/3 in HEK293/HeLa/SH-SY5Y cell lines as reported on

http://www.proteinatlas.org/. (Middle) WB analysis demonstrated the total JNKs level in HEK293T/HeLa/SH-SY5Y

cells. (Right) Comparison of endogenous JNKs in three cell lines. (B) In situ labeling of JP3 at concentrations ranging

from 1 nM to 10 μM with or without pretreatment of WT inhibitor (JNK-IN-8) where indicated. Cells were treated with

DMSO or WT (10 μM, 1 h) followed by probes (0.001-10 μM, 1 h). Cells were induced with 2 μM of anisomycin for 0.5

h before harvest. The corresponding gels stained with Coomassie brilliant blue (CBB) were provided. (C) In situ

proteome profiling of JP2-TCO (1 μM, 1 h) in cells with or without pretreatment of JNK-IN-8 (10 μM, 1 h). Cells were

induced with 2 μM of anisomycin for 0.5 h before harvest. (D) The corresponding pull-down/WB (against JNKs) results

of HEK293T (left)/SH-SY5Y (right) cells labeled by JP3/JP2-TCO (0.1 or 0.5 μM, 1 h), with or without pretreatment of 10 × WT (JNK-IN-8, 1 h), followed by click with Biotin-N3 (for JP3)/Biotin-Tz2 (for JP2-TCO/-CP). 2 μM of anisomycin (0.5 h) was used to induce the activation of JNKs after incubation with JNK probes. “Before pull-down

(BP)” samples were analyzed simultaneously to indicate the JNK levels before enrichment.

Table S3. Summary of putative hits identified from standard PD/LC-MS by using 1 μM of JP3/JP2-TCO/JP2-CP

for 1 h in anisomycin-induced (2 μM, 0.5 h) HeLa cells. The putative hits were proteins exclusively present in

http://www.proteinatlas.org/

S1_26

probe-treated cells but not in the DMSO control. Two putative common hits enriched by all three probes were listed

below (highlighted in red), and in Fig. 3 in the maintext.

Accession Description GeneMW

[kDa]

JP3 JP2-TCO JP2-CPHits of

threeScore A6 Score B6 Hits Score A6 Score B6 Hits Score A6 Score B6 Hits

IPI00220382.2Isoform Alpha-1 of Mitogen-activated

protein kinase 9MAPK9 44 9.04 12.99 Yes 0 10.93 Yes 0 6.49 Yes Yes

IPI00939219.1 Equilibrative nucleoside transporter 1 SLC29A1 50.2 7.52 18.62 Yes 2.34 3.01 Yes 7.47 2.61 Yes Yes

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[2] J. Ge, C.-W. Zhang, X. W. Ng, B. Peng, S. Pan, S. Du, D. Wang, L. Li, K. L. Lim, T. Wohland, S. Q. Yao, Angew.

Chem. Int. Ed. 2016, 55, 4933-4937.

[3] K. Tishinov, K. Schmidt, D. Haussinger, D. G. Gillingham, Angew. Chem. Int. Ed. 2012, 51, 12000-12004.

[4] J. Ge, C.-J. Zhang, L. Li, L. M. Chong, X. Wu, P. Hao, S. K. Sze, S. Q. Yao, ACS Chem. Biol. 2013, 8,

2577-2585.

[5] K. A. Kalesh, D. S. Sim, J. Wang, K. Liu, Q. Lin, S. Q. Yao, Chem. Commun. 2010, 46, 1118-1120.

[6] T. Zhang, F. Inesta-Vaquera, M. Niepel, J. Zhang, S. B. Ficarro, T. Machleidt, T. Xie, J. A. Marto, N. Kim, T.

Sim, J. D. Laughlin, H. Park, P. V. LoGrasso, M. Patricelli, T. K. Nomanbhoy, P. K. Sorger, D. R. Alessi, N. S.

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[7] M. K. Pallerla, J. M. Fox, Org. Lett. 2005, 7, 3593-3595.

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[9] A. K. Spate, H. Busskamp, A. Niederwieser, V. F. Schart, A. Marx, V. Wittmann, Bioconjug. Chem. 2014, 25,

147-154.

[10] A. Turetsky, E. Kim, R. H. Kohler, M. A. Miller, R. Weissleder, Sci. Rep. 2014, 4, 4782.

[11] J. F. McGouran, H. B. Kramer, M. M. Mackeen, K. di Gleria, M. Altun, B. M. Kessler, Org. Biomol. Chem.

2012, 10, 3379-3383.

[12] H. Takakura, K. Sasakura, T. Ueno, Y. Urano, T. Terai, K. Hanaoka, T. Tsuboi, T. Nagano, Chem. Asian. J. 2010,

5, 2053-2061.

[13] L. Li, C. W. Zhang, J. Ge, L. Qian, B. H. Chai, Q. Zhu, J. S. Lee, K. L. Lim, S. Q. Yao, Angew. Chem. Int. Ed.

2015, 54, 10821-10825.

[14] a) J. C. Carlson, L. G. Meimetis, S. A. Hilderbrand, R. Weissleder, Angew. Chem. Int. Ed. 2013, 52, 6917-6920;

b) J. Yang, M. R. Karver, W. Li, S. Sahu, N. K. Devaraj, Angew. Chem. Int. Ed. 2012, 51, 5222-5225.

[15] C.-J. Zhang, C. Y. Tan, J. Ge, Z. Na, G. Y. Chen, M. Uttamchandani, H. Sun, S. Q. Yao, Angew. Chem. Int. Ed.

2013, 52, 14060-14064.

[16] S. K. Das, C. S. Lim, S. Y. Yang, J. H. Han, B. R. Cho, Chem. Commun. 2012, 48, 8395-8397.

[17] J. Wu, J. Yu, Y. Wang, P. Zhang, Synlett. 2013, 24, 1448-1454.

[18] C. Chung, D. Srikun, C. S. Lim, C. J. Chang, B. R. Cho, Chem. Commun. 2011, 47, 9618-9620.

[19] a) Z. Li, P. Hao, L. Li, C. Y. Tan, X. Cheng, G. Y. Chen, S. K. Sze, H. M. Shen, S. Q. Yao, Angew. Chem. Int. Ed.

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S1_27

5829-5833.

S1_28

Appendix (1H and 13C NMR spectra & HRMS Data)

S1_47

(JP2-TCO)

S1_48

(JP2-CP)

S1_49

(JP3)

S1_50

(QT2)

S1_51

(QT1)

Excel S1_Standard MS Results_HeLa.xlsx Protocol

Protocol for MS analysis

Cell experiment and data analysis

LC-MS analysis was performed on a nanoAcquity (Waters, inc) configured with Q-exactive Orbitrap mass spectrometer (ThermoScientific Inc.). Digests were loaded onto a fused silica desalting column packed with 2 cm C18 reverse phase resin (Phenomenex Inc.), and

peptides were eluted on a reverse phase column (100 μm fused silica packed with 25 cm C18 resin) using a gradient 5-50% Buffer B in Buffer A (Buffer A: water, 0.1 % formic acid; Buffer B: acetonitrile, 0.1% formic acid). The flow rate through the column was set to 250

nl min-1and spray voltage was set to 1.6 kV. One full MS scan (350 – 1800 MW) was followed by 10 data dependent scans of most intense ions with dynamic exclusion enabled. The generated tandem MS data was searched using IPI Human database (version: 3.87)

using Proteome Discoverer software (v.1.4.0.288). A maximum of three missed cleavages were allowed and fully tryptic peptides were considered with dynamic modification of acetyl at N-terminal. The maximal mass tolerance in MS mode was set to 10 ppm, and

fragment MS/MS tolerance was set to 0.8 Da for HCD data. The maximum peptide and site false discovery rates were specified as 0.01 using Percolator. Quantitative SILAC ratio was calculated using Proteome Discoverer "Precursor Ions Quantifier" modules. Lastly, gene

ontology analysis was performed using Gene Ontology Consortium (http://geneontology.org).

HeLa cells grown in 10-cm dishes were incubated with 1 μM of JP3/JP2-TCO/JP2-CP for 1 h before induction of 2 μM of anisomycin for additional 0.5 h to activate JNKs. Cells treated with DMSO were carried out concurrently as controls. Putative Hits of the probe are

targets that were enriched and detected in probe-treated sample but not in the DMSO control. (JNK1/2/3 (also known as Mitogen-activated protein kinase 8/9/10) were highlighted in red).

SI_II(1)

Excel S1_Standard MS Results_HeLa.xlsx Summary of three probes

Accession Description Gene_Symbol MW [kDa] Score A6 Score B6 JP3 Hits Score A6 Score B6 JP2-TCO Hits Score A6 Score B6 JP2-CP Hits

IPI00220382.2 Isoform Alpha-1 of Mitogen-activated protein kinase 9 MAPK9 44 9.04 12.99 Yes 0 10.93 Yes 0 6.49 Yes Yes

IPI00939219.1 Equilibrative nucleoside transporter 1 SLC29A1 50.2 7.52 18.62 Yes 2.34 3.01 Yes 7.47 2.61 Yes Yes

IPI00394788.4 Alanyl-tRNA synthetase, mitochondrial AARS2 107.3 5.01 3.21 Yes 0 2.17 Yes No

IPI00216267.2 Isoform 8 of Multidrug resistance-associated protein 1 ABCC1 151.6 4.65 9 Yes 0 13.48 Yes No

IPI00030363.1 Acetyl-CoA acetyltransferase, mitochondrial ACAT1 45.2 8.56 4.2 Yes 0 1.71 Yes No

IPI00983658.1 adenosine kinase isoform d ADK 34.1 0 5.93 Yes 0 2.55 Yes No

IPI00654746.1 Rho GTPase-activating protein 11B ARHGAP11B 30.2 1.91 0 Yes 0 1.7 Yes No

IPI00646144.3

ATPase family AAA domain-containing protein 3A isoform

3 ATAD3A 57.9 9.09 21.36 Yes 0 7.52 Yes No

IPI00217121.1 Uncharacterized protein C19orf21 C19orf21 75.3 6.33 10.67 Yes 0 0 Yes No

IPI00939370.1 CKAP4 protein (Fragment) CKAP4 67.8 12.07 5.35 Yes 0 8.87 Yes No

IPI01022580.1 14 kDa protein CS 14.1 2.99 7.17 Yes 0 2.54 Yes No

IPI00290142.5 CTP synthase 1 CTPS 66.6 10.4 13.6 Yes 0 3.7 Yes No

IPI00985384.1 ATP-dependent RNA helicase DDX3X isoform 3 DDX3X 71.3 19.56 20.85 Yes 1.77 5.12 Yes No

IPI00216099.3 Isoform 1A of Desmocollin-1 DSC1 99.9 1.67 0 Yes 0 0 Yes No

IPI00000695.1 Guanine nucleotide-binding protein subunit alpha-14 GNA14 41.5 0 2.74 Yes 0 1.75 Yes No

IPI00479145.3 Keratin, type I cytoskeletal 19 KRT19 44.1 39.64 44.19 Yes 15.06 43.04 Yes No

IPI00514738.2 Novel protein NT5DC1 12.7 0 3.06 Yes 0 5.97 Yes No

IPI00218528.1 Isoform 1 of Plakophilin-1 PKP1 80.4 3.66 6.03 Yes 0 1.71 Yes No

IPI00984352.1 Uncharacterized protein PRMT1 37.7 4.94 17.56 Yes 0 2.14 Yes No

IPI00218280.3 Isoform 2 of Replication factor C subunit 2 RFC2 35.2 3.13 5.71 Yes 0 2.68 Yes No

IPI00375570.1 Isoform 2 of Scavenger receptor class B member 1 SCARB1 45.1 3.41 3.27 Yes 0 2.21 Yes No

IPI01022950.1 Uncharacterized protein SLC25A3 36.1 1.98 5.26 Yes 0 3.32 Yes No

IPI00927101.1 Uncharacterized protein

SNORA6;RPSAP15;S

NORA62;RPSA 29.5 0 9.88 Yes 0 3.73 Yes No

IPI00012505.7 Isoform 3 of Transmembrane protease serine 13 TMPRSS13 57.1 0 1.79 Yes 0 0 Yes No

IPI00419856.1 transportin-2 isoform 3 TNPO2 100.3 1.69 13.33 Yes 0 4.08 Yes No

IPI01022358.1 Alpha-tubulin TUBA1A 46.3 36.85 94.53 Yes 2.04 23.03 Yes No

IPI00013683.2 Tubulin beta-3 chain TUBB3;MC1R 50.4 51.77 93.1 Yes 0 34.53 Yes No

IPI01010637.1

cDNA FLJ39753 fis, clone SMINT2018343, highly similar to

Synaptic vesicle membrane protein VAT-1homolog VAT1 32.5 7.16 5.44 Yes 0 4.81 Yes No

IPI00871622.2 Putative zinc-alpha-2-glycoprotein-like 1 - 23 0 3.39 Yes 0 5.09 Yes No

IPI01014163.1 Putative metaphase chromosome protein 1 - 33 0 2.39 Yes No

IPI00394838.3 cDNA FLJ56442, highly similar to ATP-citrate synthase ACLY 125.1 0 3.14 Yes No

IPI00908458.1 cDNA FLJ58087, highly similar to Alpha-actinin-4 ACTN4 64.1 0 5.51 Yes No

IPI00935818.1 adenosylhomocysteinase isoform 2 AHCY 44.6 0 1.75 Yes 0 3.49 Yes No

IPI00021812.2 Neuroblast differentiation-associated protein AHNAK AHNAK 628.7 1.84 5.98 Yes No

IPI01022677.1 26 kDa protein ALDH7A1 25.9 0 3.27 Yes No

IPI00025278.1 Arachidonate 12-lipoxygenase, 12R-type ALOX12B 80.3 0 3.34 Yes 0 3.45 Yes No

IPI00455315.4 Isoform 1 of Annexin A2 ANXA2 38.6 0 18.13 Yes No

IPI00646182.5

sodium/potassium-transporting ATPase subunit alpha-1

isoform c ATP1A1 112.9 0 2.48 Yes No

IPI01022616.1 Uncharacterized protein ATP2A2 18 0 2.18 Yes No

IPI01021250.1 Protein ATP5B 38.2 0 6.68 Yes 0 5.19 Yes No

IPI00889588.1 Uncharacterized protein ATXN10 14.2 0 3.01 Yes No

IPI00026241.1 Bone marrow stromal antigen 2 BST2 19.8 2.62 2.97 Yes No

IPI00893174.1 Uncharacterized protein BZW2 26.2 0 12.45 Yes 3.2 8.67 Yes No

IPI00061492.3 Uncharacterized protein C7orf30 C7orf30 26.2 0 1.93 Yes No

IPI00465436.4 Catalase CAT 59.7 0 2.96 Yes No

IPI01022121.1 Uncharacterized protein CCT2 44.8 0 7.75 Yes No

IPI01015917.1 T-complex protein 1 subunit delta CCT4 42.3 0 2.38 Yes No

IPI01014868.1 T-complex protein 1 subunit eta isoform b CCT7 37.5 0 1.89 Yes No

IPI00794673.2

cDNA FLJ59382, highly similar to T-complex protein 1

subunit theta CCT8 51.6 0 5.7 Yes No

IPI00657796.1 Uncharacterized protein CD9 17.8 0 2.84 Yes 0 2.36 Yes No

IPI01011294.1

cDNA FLJ59465, highly similar to Cleft lip and palate

transmembrane protein 1 CLPTM1 65.6 0 1.94 Yes No

IPI00455383.4 Isoform 2 of Clathrin heavy chain 1 CLTC 187.8 0 4.71 Yes 0 1.91 Yes No

IPI00219762.5 Uncharacterized protein CSE1L 103.8 0 8.67 Yes No

IPI00794081.1 Uncharacterized protein CSTA 7.1 0 2.54 Yes 0 3.6 Yes No

IPI00892793.1 20 kDa protein CTSD 20.4 0 6.57 Yes No

IPI00384122.5

cDNA FLJ55034, highly similar to Dihydrolipoyllysine-

residue succinyltransferase component of 2- oxoglutarate

dehydrogenase complex, mitochondrial DLST;DLSTP1 39.5 0 4.85 Yes No

IPI00937615.2 Elongation factor 1-gamma EEF1G 50.1 0 8.65 Yes No

IPI00186290.6 Elongation factor 2 EEF2 95.3 0 8.83 Yes 0 6.63 Yes No

IPI01013021.1

cDNA FLJ52478, highly similar to Eukaryotic translation

initiation factor 3 subunit 5 EIF3F 23.2 0 1.82 Yes No

IPI01008914.1 eukaryotic initiation factor 4A-I isoform 2 EIF4A1 39.5 0 8.52 Yes No

IPI00009328.4 Eukaryotic initiation factor 4A-III EIF4A3 46.8 0 3 Yes No

IPI00465248.5 Isoform alpha-enolase of Alpha-enolase ENO1 47.1 1.74 8.91 Yes No

IPI00007940.6 erlin-1 ERLIN1 39.1 1.87 0 Yes No

IPI00456750.2 Isoform 2 of Niban-like protein 1 FAM129B 82.6 0 6.98 Yes No

IPI00026781.3 Fatty acid synthase FASN 273.3 0 5.66 Yes No

IPI00644576.1 Uncharacterized protein FLNA 276.4 2.98 23.36 Yes 19.46 27.5 Yes No

IPI00000885.1 Tyrosine-protein kinase FRK FRK 58.2 0 3.87 Yes No

IPI01012526.1 Uncharacterized protein GANAB 96.2 0 1.89 Yes No

IPI00789134.5 Glyceraldehyde-3-phosphate dehydrogenase GAPDH 27.9 0 2.81 Yes 0 3.76 Yes No

IPI00916612.1 gamma-glutamylcyclotransferase isoform 4 GGCT 10.5 0 4.05 Yes No

IPI00248911.8 Guanine nucleotide-binding protein G(t) subunit alpha-3 GNAT3 40.3 0 2.3 Yes No

IPI00220855.3 Isoform 1 of Histone H2A.J H2AFJ 14 0 2.05 Yes No

IPI00889691.3 Uncharacterized protein HEATR7A 180.2 0 1.73 Yes No

IPI00003935.6 Histone H2B type 2-E HIST2H2BE 13.9 0 1.72 Yes No

IPI00026824.2 Heme oxygenase 2 HMOX2 41.6 0 0 Yes No

IPI00396378.3

Isoform B1 of Heterogeneous nuclear ribonucleoproteins

A2/B1 HNRNPA2B1 37.4 0 1.86 Yes No

IPI00514561.1

cDNA FLJ54552, highly similar to Heterogeneous nuclear

ribonucleoprotein K HNRNPK 47.5 0 2.03 Yes 4.67 4 Yes No

IPI00027834.3 Heterogeneous nuclear ribonucleoprotein L HNRNPL 64.1 0 3.82 Yes No

IPI01014861.1

Heterogeneous nuclear ribonucleoprotein M isoform a

variant (Fragment) HNRNPM 65.2 0 8.36 Yes No

IPI00644079.4

cDNA FLJ44920 fis, clone BRAMY3011501, highly similar to

Heterogeneous nuclear ribonucleoprotein U HNRNPU 83 0 4.12 Yes 0 2.51 Yes No

IPI00911039.1

cDNA FLJ54408, highly similar to Heat shock 70 kDa

protein 1 HSPA1B;HSPA1A 63.9 5.08 17.13 Yes No

IPI00002966.2 Heat shock 70 kDa protein 4 HSPA4 94.3 0 2.19 Yes No

IPI00922694.2 Uncharacterized protein HSPA9 69.9 0 5.72 Yes 0 2.95 Yes No

IPI00924999.1 Uncharacterized protein IMPDH2 30.6 2.4 0 Yes No

IPI00007402.3 Importin-7 IPO7 119.4 0 5.12 Yes No

IPI00009342.1 Ras GTPase-activating-like protein IQGAP1 IQGAP1 189.1 0 6.76 Yes 0 4.54 Yes No

IPI00293305.4 Isoform Beta-1B of Integrin beta-1 ITGB1 87.4 0 3.76 Yes No

IPI00001639.2 Importin subunit beta-1 KPNB1 97.1 0 8.64 Yes No

IPI00514908.1 Keratinocyte proline-rich protein KPRP 64.1 0 1.92 Yes No

IPI00796776.2

cDNA FLJ54081, highly similar to Keratin, type II

cytoskeletal 5 KRT5 58.8 26.38 72.13 Yes No

IPI00306959.12 Keratin, type II cytoskeletal 7 KRT7 51.4 2.26 35.21 Yes 16.07 21.71 Yes No

IPI01021596.1 Uncharacterized protein KRT8 11.3 0 0 Yes No

IPI00431749.2 Isoform 2 of Keratin, type II cytoskeletal 80 KRT80 47.2 0 0 Yes No

IPI00182655.8 Keratin, type II cuticular Hb6 KRT86 53.5 4.56 22.75 Yes No

IPI01014783.1

cDNA FLJ58533, highly similar to Leucyl-tRNA synthetase,

cytoplasmic LARS 128 0 2.55 Yes No

IPI00216952.1 Isoform C of Prelamin-A/C LMNA 65.1 0 5.27 Yes No

IPI00783271.1 Leucine-rich PPR motif-containing protein, mitochondrial LRPPRC 157.8 0 7.93 Yes 0 5.94 Yes No

IPI00016334.2 Isoform 1 of Cell surface glycoprotein MUC18 MCAM 71.6 0 5.18 Yes 0 3.78 Yes No

JP3 (Standard LCMS) JP2-TCO (Standard LCMS) JP2-CP (Standard LCMS) Putative Hits of

three probes

SI_II(2)


IPI00024580.4

Methylcrotonoyl-CoA carboxylase subunit alpha,

mitochondrial MCCC1 80.4 2.56 19.49 Yes No

IPI00031517.1 DNA replication licensing factor MCM6 MCM6 92.8 0 3.19 Yes No

IPI00219740.3 Isoform 2 of DNA replication licensing factor MCM7 MCM7 44.6 0 2.52 Yes No

IPI00218342.11 C-1-tetrahydrofolate synthase, cytoplasmic MTHFD1 107.4 0 7.43 Yes No

IPI00980291.1 Uncharacterized protein MUC6 19.3 0 0 Yes No

IPI00019502.3 Isoform 1 of Myosin-9 MYH9 226.4 0 5.64 Yes No

IPI00299524.2 Condensin complex subunit 1 NCAPD2 157.1 1.68 0 Yes No

IPI00878551.2

cDNA FLJ59430, highly similar to Protein disulfide-

isomerase P4HB 51.2 1.65 10.03 Yes 0 5.33 Yes No

IPI00642944.1 Uncharacterized protein PABPC4 67.9 0 3.43 Yes 0 3.76 Yes No

IPI00639981.1 Uncharacterized protein PFKP 26.1 0 4.3 Yes No

IPI01011970.1 6-phosphogluconate dehydrogenase, decarboxylating PGD 50.8 0 2.22 Yes No

IPI01011912.1 Phosphoglycerate kinase PGK1 41.4 0 1.95 Yes No

IPI00642548.2 Uncharacterized protein PHGDH 53.1 0 8.13 Yes No

IPI00398776.3 Isoform 7 of Plectin PLEC 512.3 1.8 1.84 Yes 1.82 8.58 Yes No

IPI00924722.1 Uncharacterized protein PMPCB 22.1 0 1.83 Yes No

IPI01012180.1 Uncharacterized protein PODXL 54.4 0 2.47 Yes No

IPI00641244.1 11 kDa protein PRDX1 10.7 0 1.71 Yes 0 4.1 Yes No

IPI00760871.1 Isoform 2 of Prolyl endopeptidase-like PREPL 76.3 0 0 Yes No

IPI00376215.2

Isoform 2 of DNA-dependent protein kinase catalytic

subunit PRKDC 465.2 0 6.15 Yes No

IPI00947213.1 Uncharacterized protein PRPS2 16.1 0 1.95 Yes No

IPI00297261.3 Tyrosine-protein phosphatase non-receptor type 1 PTPN1 49.9 0 2.18 Yes No

IPI00759723.1

Isoform Monomeric of Arginyl-tRNA synthetase,

cytoplasmic RARS 67.1 0 5.96 Yes No

IPI00791426.2 Uncharacterized protein RPL24 14.4 0 3.37 Yes No

IPI00219160.3 60S ribosomal protein L34 RPL34 13.3 0 2.23 Yes 0 1.66 Yes No

IPI00908662.3

cDNA FLJ50994, moderately similar to 60S ribosomal

protein L4 RPL4 27 0 2.25 Yes No

IPI01023074.1 Uncharacterized protein RPLP0 12.2 0 2.23 Yes No

IPI01013317.1 40S ribosomal protein S27a RPS27A 15.3 0 1.9 Yes No

IPI00643522.1 40S ribosomal protein S6 RPS6 25 0 4.41 Yes No

IPI00966870.1 Uncharacterized protein RPS6KA2 16.4 0 0 Yes No

IPI00645201.1 Uncharacterized protein RPS8;SNORD55 21.9 0 2.49 Yes 0 2.7 Yes No

IPI01014133.1

cDNA FLJ32936 fis, clone TESTI2007533, highly similar to

RuvB-like 2 RUVBL2 46.3 0 2.14 Yes No

IPI00027462.1 Protein S100-A9 S100A9 13.2 0 0 Yes 5.39 3.65 Yes No

IPI00853290.2 Uncharacterized protein SEC31A 96.4 0 2.17 Yes No

IPI00909237.1

cDNA FLJ55703, highly similar to Solute carrier family 2,

facilitated glucose transporter member 1 SLC2A1 47.8 0 2.59 Yes 0 2.9 Yes No

IPI00871279.1 Uncharacterized protein SLC38A1 58.7 0 2.54 Yes No

IPI00413265.4 Structural maintenance of chromosomes protein 5 SMC5 128.7 0 0 Yes No

IPI00552414.4 Isoform 1 of Sperm flagellar protein 1 SPEF1 27 0 0 Yes No

IPI00297492.2

Dolichyl-diphosphooligosaccharide--protein

glycosyltransferase subunit STT3A STT3A 80.5 0 4.27 Yes No

IPI00247295.4 Isoform 4 of Nesprin-1 SYNE1 1004.6 0 3.84 Yes 2.09 2.15 Yes No

IPI01013460.1

cDNA FLJ58187, highly similar to Protein-glutamine

gamma-glutamyltransferase 2 TGM2 68.6 0 2.12 Yes 0 2.22 Yes No

IPI00909882.1

cDNA FLJ57574, highly similar to Protein-glutamine

gamma-glutamyltransferase E TGM3 21.4 0 1.99 Yes No

IPI00940673.2

cDNA FLJ36348 fis, clone THYMU2007025, highly similar to

TRANSKETOLASE TKT 58.9 1.72 7.55 Yes 0 2.6 Yes No

IPI00830089.1 Thymopoietin zeta isoform TMPO 27.4 0 2.06 Yes No

IPI00020416.8 Tripeptidyl-peptidase 2 TPP2 138.3 0 1.91 Yes No

IPI00643876.2

cDNA FLJ50466, highly similar to DNA (cytosine-5)-

methyltransferase-like protein 2 TRDMT1 35.1 0 0 Yes No

IPI00847729.3 Isoform 2 of Pachytene checkpoint protein 2 homolog TRIP13 22.1 2.56 4.66 Yes No

IPI00910611.1 tetraspanin-3 isoform 3 TSPAN3 21.2 2.04 1.9 Yes No

IPI00552768.1 Uncharacterized protein TXN 9.4 0 2.55 Yes 0 3.38 Yes No

IPI00513788.2

uveal autoantigen with coiled-coil domains and ankyrin

repeats isoform 2 UACA 161.4 0 3.78 Yes No

IPI00645078.1 Ubiquitin-like modifier-activating enzyme 1 UBA1 117.8 0 6.56 Yes No

IPI00789382.1 UBASH3A protein UBASH3A 59.1 0 1.62 Yes No

IPI00964927.1 UDP-glucose 6-dehydrogenase isoform 3 UGDH 44.4 0 2.08 Yes No

IPI00022774.3 Transitional endoplasmic reticulum ATPase VCP 89.3 0 4.9 Yes No

IPI01010050.1

cDNA, FLJ78818, highly similar to Voltage-dependent

anion-selective channel protein 2 VDAC2 27.5 0 2.59 Yes No

IPI00298961.3 Exportin-1 XPO1 123.3 0 3.39 Yes No

IPI00028357.2 Exportin-4 XPO4 130.1 0 2.13 Yes No

IPI00974544.1 Isoform SV of 14-3-3 protein epsilon YWHAE 26.5 0 1.95 Yes No

IPI00845286.1 Isoform 1 of Zinc finger protein 618 ZNF618 104.9 0 4.27 Yes No

IPI00442304.1 cDNA FLJ16183 fis, clone BRTHA2002702 - 20.1 0 1.61 Yes 0 0 Yes No

IPI00873231.2

cDNA FLJ60768, moderately similar to RNA polymerase I-

specific transcriptioninitiation factor RRN3 - 55 0 1.99 Yes No

IPI00871956.1 Similar to Ribosomal protein S2 - 19.9 0 1.96 Yes No

IPI00166157.1 CDNA FLJ25625 fis, clone STM02974 - 21 0 1.8 Yes No

IPI00975598.1 Conserved hypothetical protein - 16.9 0 0 Yes No

IPI00746069.1 Conserved hypothetical protein - 24 0 2.08 Yes No

IPI00910960.1

cDNA FLJ58934, moderately similar to G1/S-specific cyclin-

D3 - 15.8 0 0 Yes No

IPI00911048.1

cDNA FLJ56666, highly similar to Adhesion-regulating

molecule 1 - 20.8 0 1.68 Yes No

IPI00983798.1 Conserved hypothetical protein - 8.1 0 2.73 Yes No

IPI00911024.1

cDNA FLJ57708, highly similar to DNA-directed RNA

polymerase I 40 kDa polypeptide - 17.3 0 4.4 Yes No

IPI00980462.1 Conserved hypothetical protein - 6.8 0 2.1 Yes No

IPI00909336.1 cDNA FLJ59092 - 30.5 1.71 2.93 Yes No

IPI01022062.1 14 kDa protein - 14.4 1.88 10.68 Yes No

IPI00985292.1 BCR-ABL1 e8a2 variant (Fragment) - 50.1 1.89 4.22 Yes No

IPI00977334.1 9 kDa protein - 9.3 1.89 1.62 Yes No

IPI00885017.1

cDNA, FLJ79527, moderately similar to Uroporphyrinogen

decarboxylase - 12.9 2.08 2.39 Yes No

IPI00856098.2 p180/ribosome receptor - 165.6 8.97 11.1 Yes No

IPI00908824.1 Serine hydroxymethyltransferase - 41.6 17.09 19.34 Yes No

IPI01025580.1 Possible J 56 gene segment (Fragment) - 2.2 28 148.03 Yes No

IPI00240503.7 Uncharacterized protein - 48.2 58.86 76.96 Yes No

IPI01022604.1 Uncharacterized protein AAAS 45.6 0 8.37 Yes No

IPI00789348.1 Isoform 3 of Acetoacetyl-CoA synthetase AACS 30 0 0 Yes No

IPI00027442.4 Alanyl-tRNA synthetase, cytoplasmic AARS 106.7 16.67 11.94 Yes No

IPI00065486.3

Isoform 2 of ATP-binding cassette sub-family B member 6,

mitochondrial ABCB6 88.5 3.92 4.81 Yes No

IPI00006674.1

Isoform 1 of Canalicular multispecific organic anion

transporter 2 ABCC3 169.2 4.06 2.04 Yes No

IPI00002372.1 Isoform 1 of ATP-binding cassette sub-family D member 3 ABCD3 75.4 0 5.11 Yes No

IPI00303207.3 ATP-binding cassette sub-family E member 1 ABCE1 67.3 4.34 6.92 Yes No

IPI01015998.1 ATP-binding cassette, sub-family F, member 1 variant ABCF1 67.6 0 6.98 Yes No

IPI00005045.1 ATP-binding cassette sub-family F member 2 ABCF2 71.2 2.9 6.5 Yes No

IPI00784776.1 Isoform 2 of ATP-binding cassette sub-family F member 3 ABCF3 78.9 1.64 4.41 Yes No

IPI00744789.2 Isoform 4 of Actin-binding LIM protein 1 ABLIM1 46.1 2.45 3.91 Yes No

IPI00001539.8 3-ketoacyl-CoA thiolase, mitochondrial ACAA2 41.9 2.41 5.72 Yes No

IPI00152981.1 Acyl-CoA dehydrogenase family member 9, mitochondrial ACAD9 68.7 0 2.13 Yes No

IPI01015888.1

cDNA, FLJ78845, highly similar to Medium-chain specific

acyl-CoA dehydrogenase, mitochondrial ACADM 42.4 8.6 3.08 Yes No

IPI00178744.5

Isoform 2 of Very long-chain specific acyl-CoA

dehydrogenase, mitochondrial ACADVL 68 3.94 22.07 Yes No

IPI00291419.6

cDNA FLJ53975, highly similar to Acetyl-CoA

acetyltransferase, cytosolic ACAT2 44.6 0 6.9 Yes No

SI_II(3)


IPI00983296.2 Uncharacterized protein ACLY 126.1 57.13 97.52 Yes No

IPI00008485.1 Cytoplasmic aconitate hydratase ACO1 98.3 6.7 3.56 Yes No

IPI00909879.1

cDNA FLJ50886, highly similar to Aconitate hydratase,

mitochondrial ACO2 54.4 5.25 6.4 Yes No

IPI00883762.1 Isoform 2 of Cytosolic acyl coenzyme A thioester ACOT7 27 3.27 3.33 Yes No

IPI00921986.1

Isoform 2 of Acyl-coenzyme A thioesterase 9,

mitochondrial ACOT9 46.3 2.41 9.34 Yes No

IPI00982418.1

cDNA FLJ53511, highly similar to Long-chain-fatty-acid--

CoA ligase 1 ACSL1 58.5 1.69 0 Yes No

IPI00031397.3 Long-chain-fatty-acid--CoA ligase 3 ACSL3 80.4 9.31 9.82 Yes No

IPI00219897.1 Isoform Short of Long-chain-fatty-acid--CoA ligase 4 ACSL4 74.4 11.81 8.19 Yes No

IPI00922693.1

cDNA FLJ53662, highly similar to Actin, alpha skeletal

muscle ACTB 38.6 97.17 116.82 Yes No

IPI00003269.1 Beta-actin-like protein 2 ACTBL2 42 76.65 99.01 Yes No

IPI00003627.1 Isoform 1 of Actin-like protein 6A ACTL6A 47.4 3.21 6.03 Yes No

IPI00013508.5 Isoform 1 of Alpha-actinin-1 ACTN1 103 75.96 86.28 Yes No

IPI00005159.3 Actin-related protein 2 ACTR2 44.7 7.42 2.43 Yes No

IPI01013413.2 Uncharacterized protein ACTR3 40.7 2.76 5.46 Yes No

IPI00760588.2

Isoform 5 of Double-stranded RNA-specific adenosine

deaminase ADAR 103.6 5.73 12.72 Yes No

IPI00965916.1 Protein ADD1 48.5 0 5.06 Yes No

IPI00220754.1 Isoform 1 of Gamma-adducin ADD3 75.6 0 2.13 Yes No

IPI00966735.1 28 kDa protein ADH5;ADH5P4 27.5 2.22 4.39 Yes No

IPI00220887.1 Isoform 2 of Adenylosuccinate lyase ADSL 48.3 2.54 4.19 Yes No

IPI00641992.5 Uncharacterized protein ADSS 47.9 3.67 7.93 Yes No

IPI00001091.4 AFG3-like protein 2 AFG3L2 88.5 0 9.22 Yes No

IPI00945912.1

cDNA FLJ59963, highly similar to Homo sapiens multiple

substrate lipid kinase (MULK), mRNA AGK 26.9 0 4.28 Yes No

IPI00219065.2 Isoform 5 of Glycogen debranching enzyme AGL 172.5 3.89 1.96 Yes No

IPI00010349.1 Alkyldihydroxyacetonephosphate synthase, peroxisomal AGPS 72.9 5.02 5.95 Yes No

IPI00374563.3 Agrin AGRN 214.7 0 3.73 Yes No

IPI00954159.1 Isoform 1 of Protein ELYS AHCTF1P1;AHCTF1 252.3 4.26 4.14 Yes No

IPI00479201.3 Isoform 2 of Putative adenosylhomocysteinase 2 AHCYL1 53.7 5.16 11.82 Yes No

IPI00030706.1 Activator of 90 kDa heat shock protein ATPase homolog 1 AHSA1 38.3 2.76 8 Yes No

IPI00157908.4 Isoform 3 of Apoptosis-inducing factor 1, mitochondrial AIFM1 66.3 0 12.97 Yes No

IPI00910647.1

cDNA FLJ52127, highly similar to Multisynthetase complex

auxiliary component p43 AIMP1 29.7 3.33 7.37 Yes No

IPI00953925.1 Aryl hydrocarbon receptor interacting protein AIP 38.5 0 3.47 Yes No

IPI00829774.1 Isoform 4 of A-kinase anchor protein 13 AKAP13 305.1 7.06 7.43 Yes No

IPI00297089.4 A-kinase anchor protein 6 AKAP6 256.6 1.65 1.87 Yes No

IPI00220271.3 Alcohol dehydrogenase [NADP+] AKR1A1 36.5 0 4.08 Yes No

IPI00413641.7 Aldose reductase AKR1B1 35.8 2.68 7.99 Yes No

IPI00455167.4 Uncharacterized protein AKR1C2 24.9 3 2.35 Yes No

IPI00012866.2 RAC-alpha serine/threonine-protein kinase AKT1 55.7 0 3.85 Yes No

IPI00794313.1 Uncharacterized protein AKT2 24.5 0 1.69 Yes No

IPI00965913.1 Protein ALB 51.5 14.76 15.21 Yes No

IPI00218547.1 Isoform Short of Delta-1-pyrroline-5-carboxylate synthase ALDH18A1 87 9.59 19.03 Yes No

IPI00241238.3 retinal dehydrogenase 2 isoform 3 ALDH1A2 46.1 0 1.91 Yes No

IPI00103467.5 Aldehyde dehydrogenase X, mitochondrial ALDH1B1 57.2 2.25 6.3 Yes No

IPI01021497.1 Uncharacterized protein ALDH2 40.8 0 1.76 Yes No

IPI00009744.3 Aldehyde dehydrogenase family 3 member B2 ALDH3B2 42.6 1.75 0 Yes No

IPI00019888.1 Succinate-semialdehyde dehydrogenase, mitochondrial ALDH5A1 57.2 0 4.66 Yes No

IPI01020665.1 Uncharacterized protein ALDH7A1 51.4 5.36 5.06 Yes No

IPI00465439.5 Fructose-bisphosphate aldolase A ALDOA 39.4 32.5 41.29 Yes No

IPI00418262.5 Fructose-bisphosphate aldolase ALDOC 48.4 10.69 6.59 Yes No

IPI00395632.6 ALK tyrosine kinase receptor ALK 176.3 9.01 1.76 Yes No

IPI00942246.1 Isoform Ex1B-2-3 of AMP deaminase 2 AMPD2 100.6 4.14 3.69 Yes No

IPI01022592.1 Uncharacterized protein ANAPC7 23.3 0 0 Yes No


ANKHD1-

EIF4EBP3;ANKHD1 268.6 0 6.91 Yes No

IPI01011146.1 Uncharacterized protein ANKLE2 97.7 2.31 3.68 Yes No

IPI00908701.1

cDNA FLJ54784, highly similar to Ankyrin repeat domain-

containing protein 28 ANKRD28 77.7 0 2.33 Yes No

IPI00743594.2 Actin-binding protein anillin ANLN 124.2 5.49 4.21 Yes No

IPI00221224.6 Aminopeptidase N ANPEP 109.5 1.93 3.12 Yes No

IPI00218918.5 Annexin A1 ANXA1 38.7 30.77 27.89 Yes No

IPI00964070.1 Uncharacterized protein ANXA3 32.1 7.52 9.46 Yes No

IPI00967416.1 Uncharacterized protein ANXA5 24.7 1.7 4.72 Yes No

IPI01011531.3 cDNA FLJ54149, highly similar to Annexin A7 ANXA7 37.8 3.79 3.84 Yes No


ANXA8L2;ANXA8L1;

ANXA8 30 0 0 Yes No

IPI00940292.1 Isoform C of AP-1 complex subunit beta-1 AP1B1 103.5 18.71 11.3 Yes No

IPI00939879.1 Isoform 1 of AP-1 complex subunit gamma-1 AP1G1 91.3 3.95 6.68 Yes No

IPI00032516.5 AP-1 complex subunit mu-1 AP1M1 48.6 0 4.45 Yes No

IPI00256684.2 Isoform B of AP-2 complex subunit alpha-1 AP2A1 105.3 15.36 18.29 Yes No

IPI00790702.1 Uncharacterized protein AP2B1 98.1 18.31 23.62 Yes No

IPI00925072.1 Uncharacterized protein AP2M1 28.8 0 3.65 Yes No

IPI00982231.1 Uncharacterized protein AP3B1 116.1 2.05 9.4 Yes No

IPI00411677.3 Isoform 4 of AP-3 complex subunit delta-1 AP3D1 114.6 0 2.49 Yes No

IPI00294556.2

Amyloid beta A4 precursor protein-binding family A

member 1 APBA1 92.8 0 1.95 Yes No

IPI00337741.4 Acylamino-acid-releasing enzyme APEH 81.2 0 14.38 Yes No

IPI01025023.1 16 kDa protein APEX1 15.9 2.85 2.46 Yes No

IPI00978005.1 cDNA FLJ51754, highly similar to Apoptosis inhibitor 5 API5 57.9 1.77 1.73 Yes No

IPI01014270.2 Uncharacterized protein APOB 489.6 4.64 5.65 Yes No

IPI00956296.1 cDNA FLJ55519, highly similar to Kinesin light chain 1 APOPT1 83.6 2.87 0 Yes No

IPI00297572.6 157 kDa protein AQR 156.8 3.3 8.74 Yes No

IPI00020578.2 ARAF protein ARAF 67.9 0 2.24 Yes No

IPI00103380.1

Arf-GAP with Rho-GAP domain, ANK repeat and PH

domain-containing protein 3 ARAP3 169.7 2.01 1.73 Yes No

IPI00514053.1 Coatomer subunit delta ARCN1 57.2 2.19 9.78 Yes No

IPI00922812.1

cDNA FLJ54156, highly similar to ADP-ribosylation factor

GTPase-activating protein 3 ARFGAP3 48.9 0 1.97 Yes No

IPI00020567.3 Rho GTPase-activating protein 1 ARHGAP1 50.4 0 3.74 Yes No

IPI00386947.4 Isoform 2 of Rho GTPase-activating protein 17 ARHGAP17 87.6 5.76 6.96 Yes No

IPI00760924.1 Isoform 2 of Rho GTPase-activating protein 18 ARHGAP18 70.1 2.11 0 Yes No

IPI00718985.1 Isoform 2 of Rho GTPase-activating protein 35 ARHGAP35 170.4 0 3.53 Yes No

IPI00013988.2 Isoform 2 of Rho GTPase-activating protein 5 ARHGAP5 172.2 0 6.25 Yes No

IPI00339379.3 Isoform 2 of Rho guanine nucleotide exchange factor 1 ARHGEF1 98.7 1.68 4.92 Yes No

IPI00981196.1 Isoform 3 of Rho guanine nucleotide exchange factor 10 ARHGEF10 144.2 2.62 0 Yes No

IPI00339353.3 Isoform 2 of Rho guanine nucleotide exchange factor 12 ARHGEF12 171 0 1.71 Yes No

IPI00940816.2 Isoform 3 of Rho guanine nucleotide exchange factor 2 ARHGEF2 108.2 3.94 5.51 Yes No

IPI00472891.3 cDNA FLJ90459 fis, clone NT2RP3001754 ARID1A 83.5 0 2.67 Yes No

IPI00216484.6 Isoform 2 of LisH domain-containing protein ARMC9 ARMC9 75.5 2.2 1.86 Yes No

IPI00009906.5 Armadillo repeat-containing X-linked protein 3 ARMCX3 42.5 1.78 2.36 Yes No

IPI00983530.1 actin-related protein 2/3 complex subunit 1A isoform 2 ARPC1A 39.6 2.27 0 Yes No

IPI00005160.3 Actin-related protein 2/3 complex subunit 1B ARPC1B;ARPC1A 40.9 0 3.71 Yes No

IPI00926232.1 Uncharacterized protein ARPC4;ARPC4-TTLL3 13 0 2.2 Yes No

IPI00983035.1 Uncharacterized protein ASCC1 14.2 0 2.36 Yes No

IPI00452803.5

Isoform 2 of Activating signal cointegrator 1 complex

subunit 2 ASCC2 41.8 0 2.48 Yes No

IPI00430472.2 Activating signal cointegrator 1 complex subunit 3 ASCC3 251.3 8.2 4.93 Yes No

IPI00956483.1

N-acetylserotonin O-methyltransferase-like protein

isoform 2 ASMTL 62.4 3.78 2.05 Yes No

IPI00013466.4 ATPase ASNA1 ASNA1 38.8 0 5.83 Yes No

IPI00925572.1 asparagine synthetase [glutamine-hydrolyzing] isoform b ASNS 62.1 0 8.5 Yes No

IPI00944640.1 aspartyl/asparaginyl beta-hydroxylase isoform f ASPH 83.2 0 3.77 Yes No

SI_II(4)


IPI00743813.3

Isoform 1 of Abnormal spindle-like microcephaly-

associated protein ASPM 409.5 3.68 1.63 Yes No

IPI00020632.4 Argininosuccinate synthase ASS1 50.8 8.55 8.57 Yes No

IPI00929553.1

Isoform 2 of ATPase family AAA domain-containing

protein 1 ATAD1 32.4 1.75 4.5 Yes No

IPI01012574.1 Uncharacterized protein ATL3 58.7 0 1.82 Yes No

IPI00218353.3 Isoform B of Probable cation-transporting ATPase 13A1 ATP13A1 121 6.96 0 Yes No

IPI00006482.1

Isoform Long of Sodium/potassium-transporting ATPase

subunit alpha-1 ATP1A1 112.8 11.8 23.98 Yes No

IPI01009449.1

cDNA FLJ51474, highly similar to Sodium/potassium-

transporting ATPase subunit beta-1 ATP1B1 34.1 0 5.37 Yes No

IPI00969023.1

Isoform 3 of Sarcoplasmic/endoplasmic reticulum calcium

ATPase 2 ATP2A2 109.8 17.6 15.48 Yes No

IPI00217170.1

Isoform ZB of Plasma membrane calcium-transporting

ATPase 4 ATP2B4 132.5 3.47 0 Yes No

IPI00440493.2 ATP synthase subunit alpha, mitochondrial ATP5A1 59.7 29.47 30.04 Yes No

IPI01012810.1 Uncharacterized protein ATP6V0A1 55.2 0 2.24 Yes No

IPI00943212.2 Uncharacterized protein ATP6V0D1 31.7 2.75 2.18 Yes No

IPI00946636.2

cDNA FLJ51804, highly similar to Vacuolar ATP synthase

catalytic subunit A, ubiquitous isoform ATP6V1A 64.7 9.14 4.91 Yes No

IPI00007814.3 V-type proton ATPase subunit C 1 ATP6V1C1 43.9 1.61 3.63 Yes No

IPI00978544.1 52 kDa protein ATP6V1H 51.5 0 1.93 Yes No

IPI00940152.1 ataxin-10 isoform 2 ATXN10 46.3 8.29 23.53 Yes No

IPI00021552.1 UDP-GalNAc:beta-1,3-N-acetylgalactosaminyltransferase 1 B3GALNT1 39.5 2.16 2.16 Yes No

IPI01013346.2 Uncharacterized protein B4GALT1 39.9 0 4.77 Yes No

IPI00873139.1 Isoform 2 of Beta-1,4-galactosyltransferase 3 B4GALT3 15.6 1.71 0 Yes No

IPI00793299.2 large proline-rich protein BAG6 isoform c BAG6 96.7 2.29 4.71 Yes No

IPI00216695.1 Isoform 2 of Tyrosine-protein kinase BAZ1B BAZ1B 170.3 1.87 4.97 Yes No

IPI00956389.1 cDNA FLJ53282, highly similar to Dipeptidyl-peptidase 3 BBS1 71 2.01 5.38 Yes No

IPI00909058.1

cDNA FLJ54246, highly similar to Homo sapiens BRCA2 and

CDKN1A interacting protein (BCCIP), transcript variant C,

mRNA BCCIP 29.8 0 1.74 Yes No

IPI00980443.1 Uncharacterized protein BCLAF1 83.2 3.61 3.87 Yes No

IPI00221001.1

Isoform BIN1-10-13 of Myc box-dependent-interacting

protein 1 BIN1 45.5 0 0 Yes No

IPI00219575.5 Bleomycin hydrolase BLMH 52.5 0 4.74 Yes No

IPI00294158.1 Biliverdin reductase A BLVRA 33.4 2.84 17.22 Yes No

IPI00741996.9 Uncharacterized protein BOP1 71.9 1.63 1.9 Yes No

IPI00433811.2

Isoform 2 of Short palate, lung and nasal epithelium

carcinoma-associated protein 3 BPIFA3 24.3 1.85 0 Yes No

IPI00410215.6 Isoform 2 of 3'(2'),5'-bisphosphate nucleotidase 1 BPNT1 35.7 0 2.22 Yes No

IPI00658145.3 Isoform 1 of BRCA1-associated ATM activator 1 BRAT1 88.1 1.82 2.16 Yes No

IPI00181728.1 Ribosome biogenesis protein BRX1 homolog BRIX1 41.4 0 0 Yes No

IPI00644855.3 Uncharacterized protein BROX 38.2 0 1.66 Yes No

IPI01010654.1

cDNA FLJ57899, highly similar to Mitotic checkpoint

protein BUB3 BUB3 28.2 3.64 4.95 Yes No

IPI00916503.1

cDNA FLJ55789, highly similar to Rattus norvegicus basic

leucine zipper and W2 domains 1 (Bzw1), mRNA BZW1 51.2 8.99 13.32 Yes No

IPI00975776.1 Uncharacterized protein C11orf54 8.3 0 0 Yes No

IPI01021558.1 Uncharacterized protein C12orf10 24 2.78 1.84 Yes No

IPI00166766.4 Isoform 2 of UPF0764 protein C16orf89 C16orf89 44.6 0 0 Yes No

IPI00386891.4 Isoform 1 of Uncharacterized protein C17orf53 C17orf53 69.7 1.8 0 Yes No

IPI00023078.2 Skin-specific protein 32 C1orf68 26.2 0 2.21 Yes No

IPI00791304.3 Chromosome 20 open reading frame 3 C20orf3 45.4 7.3 5.14 Yes No

IPI00910144.2 Uncharacterized protein C22orf28 52.4 2.7 5.74 Yes No

IPI00375685.2 Isoform 2 of Protein midA homolog, mitochondrial C2orf56 38 0 2.78 Yes No

IPI00002270.3 UPF0364 protein C6orf211 C6orf211 51.1 0 2.16 Yes No

IPI00796624.2 Uncharacterized protein C8orf41 53.3 1.84 4.41 Yes No

IPI00170972.2 UPF0553 protein C9orf64 C9orf64 39 5.48 0 Yes No

IPI00893035.1 Uncharacterized protein CAD 235.9 26.4 38.54 Yes No

IPI01013746.1 cDNA FLJ58668, highly similar to Calreticulin CALR 34.7 0 2.85 Yes No

IPI00377174.2

Isoform 8 of Calcium/calmodulin-dependent protein

kinase type II subunit beta CAMK2B 57.9 6.88 3.8 Yes No

IPI00176702.4

Isoform 1 of Calmodulin-regulated spectrin-associated

protein 3 CAMSAP3 134.7 0 3.82 Yes No

IPI00100160.3

Isoform 1 of Cullin-associated NEDD8-dissociated protein

1 CAND1 136.3 3.94 23.21 Yes No

IPI00442866.2 Uncharacterized protein CANX 37.4 14.4 11.72 Yes No

IPI01009563.1 Adenylyl cyclase-associated protein CAP1 49.3 0 8.22 Yes No

IPI00917562.1 Uncharacterized protein CAPG 24.1 0 1.79 Yes No

IPI00011285.1 Calpain-1 catalytic subunit CAPN1 81.8 2.39 10.75 Yes No

IPI00910745.1

cDNA FLJ58224, highly similar to Calpain-2 catalytic

subunit CAPN2 60.5 15.73 13.44 Yes No

IPI00003765.1 Calpain-7 CAPN7 92.6 2.83 1.7 Yes No

IPI00975644.1 Uncharacterized protein CAPRIN1 19.4 6.23 4.84 Yes No

IPI00026182.5 F-actin-capping protein subunit alpha-2 CAPZA2 32.9 0 5.53 Yes No

IPI00412880.2 Isoform 1 of Histone-arginine methyltransferase CARM1 CARM1 63.4 10.29 1.81 Yes No

IPI00336016.3 Probable cysteinyl-tRNA synthetase, mitochondrial CARS2 62.2 4.94 1.99 Yes No

IPI00877929.2 Isoform 3 of Peripheral plasma membrane protein CASK CASK 102 0 6.98 Yes No

IPI00220726.2 Isoform 9 of Caspase-8 CASP8 61.8 0 4.16 Yes No

IPI01012205.1

cDNA FLJ53863, highly similar to Cystathionine beta-

synthase CBS 55.8 7.01 4.74 Yes No

IPI00759686.1

Isoform 2 of Coiled-coil and C2 domain-containing protein

1A CC2D1A 103.9 5.42 5.1 Yes No

IPI00939119.1

Isoform 2 of Cell division cycle and apoptosis regulator

protein 1 CCAR1 131 1.91 1.61 Yes No

IPI00059169.4 Coiled-coil domain-containing protein 102A CCDC102A 62.6 0 0 Yes No

IPI01014743.1 cDNA FLJ44514 fis, clone UTERU3002218 CCDC132 107.9 2 4.86 Yes No

IPI00743775.1 Isoform 2 of Coiled-coil domain-containing protein 47 CCDC47 55.3 0 3.28 Yes No

IPI00981169.1 T-complex protein 1 subunit beta isoform 2 CCT2 52.7 25.87 42.67 Yes No

IPI00553185.2 T-complex protein 1 subunit gamma CCT3 60.5 23.64 42.3 Yes No

IPI01015038.1 T-complex protein 1 subunit delta CCT4 52.3 22.96 21.99 Yes No

IPI00010720.1 T-complex protein 1 subunit epsilon CCT5 59.6 24.79 34.09 Yes No

IPI00027626.3 T-complex protein 1 subunit zeta CCT6A 58 8.96 26.2 Yes No

IPI00952607.1 T-complex protein 1 subunit eta isoform d CCT7 54.8 21.96 31.91 Yes No

IPI00302925.4 59 kDa protein CCT8 59.4 46.37 66.12 Yes No

IPI00788676.1 Isoform 2 of CD109 antigen CD109 152.6 1.73 7.61 Yes No

IPI00412771.1 CD2-associated protein CD2AP 71.4 5.39 5.09 Yes No

IPI00005670.6 Cell division cycle protein 123 homolog CDC123 39.1 0 0 Yes No

IPI01013499.1 Uncharacterized protein CDC16 54.7 0 2.11 Yes No

IPI00005822.2 Isoform 1 of Cell division cycle protein 23 homolog CDC23 68.8 0 4.52 Yes No

IPI00013122.1 Hsp90 co-chaperone Cdc37 CDC37 44.4 3.71 4.57 Yes No

IPI00477763.4 Serine/threonine-protein kinase MRCK beta CDC42BPB 194.2 3.64 6.38 Yes No

IPI00465294.2 Cell division cycle 5-like protein CDC5L 92.2 0 2.8 Yes No

IPI00658202.1 cDNA FLJ53252, highly similar to Cadherin-2 CDH2 97 0 0 Yes No

IPI00215999.1 Isoform SV9 of Cyclin-dependent kinase 11B CDK11B 92.7 2.48 3.42 Yes No

IPI00414442.4 Isoform 1 of CDK5 regulatory subunit-associated protein 3 CDK5RAP3 56.9 7.47 3.44 Yes No

IPI00967990.1 Uncharacterized protein CDK7 28.5 4.08 3.53 Yes No

IPI00443485.2

Isoform 2 of CDK5 regulatory subunit-associated protein 1-

like 1 CDKAL1 54.6 0 1.7 Yes No

IPI00478390.1 Cyclin-dependent kinase inhibitor 2A, isoform 4 CDKN2A 18 0 0 Yes No

IPI00853431.1 Uncharacterized protein CECR5 23.8 0 3.29 Yes No

IPI00218388.1 Isoform 2 of CUGBP Elav-like family member 1 CELF1 51.5 2.05 1.75 Yes No

IPI00478215.2 Centrosomal protein 55kDa CEP55 24.7 1.93 0 Yes No

IPI00305304.3 Ceramide synthase 2 CERS2 44.8 0 2.32 Yes No

IPI00002557.1 Coatomer subunit gamma-2

CHD3;LOC732272;S

NORA69;COPG2 97.6 1.95 4.88 Yes No

IPI01012026.1 Uncharacterized protein CHD4 215.2 7.68 12.43 Yes No

SI_II(5)


IPI00220289.8

Isoform 1 of Chromodomain-helicase-DNA-binding protein

6 CHD6 305.2 3.38 3.81 Yes No

IPI00333010.9 Calcium homeostasis endoplasmic reticulum protein CHERP 103.6 2.25 6.11 Yes No

IPI00885057.1

Isoform 2 of Cysteine and histidine-rich domain-containing

protein 1 CHORDC1 35.3 0 7.08 Yes No

IPI00010158.3 Chromatin accessibility complex protein 1 CHRAC1 14.7 1.63 0 Yes No

IPI00719285.1 Uncharacterized protein CIT 236.5 0 1.74 Yes No

IPI00028275.2 Isoform 1 of Cytoskeleton-associated protein 5 CKAP5 225.4 2.64 5.54 Yes No

IPI01025062.1 14 kDa protein CKB 14.2 0 2.31 Yes No

IPI00910365.1

cDNA FLJ53237, highly similar to Caseinolytic peptidase B

protein CLPB 74.4 0 0 Yes No

IPI00186429.8

Isoform 2 of Cleft lip and palate transmembrane protein 1-

like protein CLPTM1L 58.3 3.92 4.94 Yes No

IPI00165579.6 Isoform 2 of Cytosolic non-specific dipeptidase CNDP2 43.8 0 2.59 Yes No

IPI00880052.1 Isoform 2 of CCR4-NOT transcription complex subunit 1 CNOT1 266.2 1.81 14.3 Yes No

IPI00925558.1 Uncharacterized protein CNOT10 67 0 5.84 Yes No

IPI00967003.1 Protein CNOT6L 52.8 0 2.66 Yes No

IPI00220993.1

Isoform CNPI of 2',3'-cyclic-nucleotide 3'-

phosphodiesterase CNP 45.1 3.79 4.49 Yes No

IPI00184821.1 Isoform 1 of Bifunctional coenzyme A synthase COASY 62.3 0 4.84 Yes No

IPI00103483.1 Negative elongation factor B COBRA1 65.7 0 1.91 Yes No

IPI00398963.4

Isoform 2 of Conserved oligomeric Golgi complex subunit

6 COG6 68.4 0 2.32 Yes No

IPI00302944.3 Isoform 4 of Collagen alpha-1(XII) chain COL12A1 324.4 4 5.01 Yes No

IPI00306322.2 Collagen alpha-2(IV) chain COL4A2 167.4 0 1.72 Yes No

IPI00295851.4 Coatomer subunit beta COPB1 107.1 5.89 14.26 Yes No

IPI00783982.1 Coatomer subunit gamma COPG 97.7 2.03 2.28 Yes No

IPI01010035.1

cDNA FLJ52928, highly similar to COP9 signalosome

complex subunit 2 COPS2 44.2 1.75 4.68 Yes No

IPI00980899.1 COP9 signalosome complex subunit 3 isoform 2 COPS3 45.7 4.57 3.67 Yes No

IPI00966482.1

cDNA FLJ36919 fis, clone BRACE2003987, highly similar to

COP9 signalosome complex subunit 4 COPS4 40.2 0 4.01 Yes No

IPI00009958.6 COP9 signalosome complex subunit 5 COPS5 37.6 0 4.56 Yes No

IPI00328619.6 ubiquinone biosynthesis monooxygenase COQ6 isoform b COQ6 48.6 1.85 4.13 Yes No

IPI00979820.1 Conserved hypothetical protein CORO1B 14.4 0 2.54 Yes No

IPI01011988.1 cDNA, FLJ79403, highly similar to Coronin-1C CORO1C 41.6 2.08 7.97 Yes No

IPI00023736.2 Coronin-2A CORO2A 59.7 0 2.62 Yes No

IPI00027078.3 Carboxypeptidase D CPD 152.8 0 1.8 Yes No

IPI00917016.2 copine-1 isoform c CPNE1 58.9 1.98 11.33 Yes No

IPI00024403.1 Copine-3 CPNE3 60.1 5.25 9.81 Yes No

IPI00093057.6 Coproporphyrinogen-III oxidase, mitochondrial CPOX 50.1 2.21 4.65 Yes No

IPI00026219.4 Cleavage and polyadenylation specificity factor subunit 1 CPSF1 160.8 0 5.35 Yes No

IPI01014911.1 Uncharacterized protein CPSF7 41.2 0 2.2 Yes No

IPI00479108.1

Isoform 2 of Carnitine O-palmitoyltransferase 1, liver

isoform CPT1A 86.2 6.07 19.97 Yes No

IPI00012912.1 Carnitine O-palmitoyltransferase 2, mitochondrial CPT2 73.7 0 7.39 Yes No

IPI00289561.3 Cytokine receptor-like factor 1 CRLF1 46.3 3.38 0 Yes No

IPI00902594.1

cDNA PSEC0091 fis, clone PLACE1001683, highly similar to

Cartilage-associated protein CRTAP 29.9 0 1.79 Yes No

IPI00885192.2 Uncharacterized protein CRTC3 62.9 2.65 2.04 Yes No

IPI00000792.1 Quinone oxidoreductase CRYZ 35.2 0 9.37 Yes No

IPI01022863.1 Citrate synthase CS 44.7 2.99 5.23 Yes No

IPI00873244.1 Isoform Short of Cold shock domain-containing protein E1 CSDE1 85.7 19.54 17.52 Yes No

IPI00219994.2 Isoform 3 of Exportin-2 CSE1L 107.7 27.7 37.89 Yes No

IPI00963961.1

cDNA FLJ55318, highly similar to Casein kinase I isoform

alpha CSNK1A1 26.7 2.28 2.24 Yes No

IPI00297767.3 Casein kinase I isoform gamma-2 CSNK1G2 47.4 3.38 3.45 Yes No

IPI00019157.3 Chondroitin sulfate proteoglycan 4 CSPG4 250.4 2.74 2.71 Yes No

IPI00644499.1 38 kDa protein CSTF1 38.4 4.32 2.15 Yes No

IPI01012987.1 Uncharacterized protein CSTF2 46.6 2.24 2.13 Yes No

IPI00015195.1 Cleavage stimulation factor subunit 3 CSTF3 82.9 2.18 2.24 Yes No

IPI00647506.1 Uncharacterized protein CTBP2 56.1 0 9.54 Yes No

IPI00981271.2 Uncharacterized protein CTNNA1 88.6 2.06 11.31 Yes No

IPI00927799.1 cDNA FLJ57878, highly similar to Beta-catenin CTNNB1 84.7 2.27 2.51 Yes No

IPI00645320.1 Isoform 3 of Beta-catenin-like protein 1 CTNNBL1 36.1 0 4.08 Yes No

IPI00219742.1 Isoform 3 of Catenin delta-1 CTNND1 90.3 0 9.41 Yes No

IPI00645702.1 CTP synthase 2 CTPS2 65.6 2.23 12.64 Yes No

IPI00014310.3 Cullin-1 CUL1 89.6 2.05 7.3 Yes No

IPI00647073.1 Uncharacterized protein CUL2 80.3 1.93 6.94 Yes No

IPI00014312.1 Isoform 1 of Cullin-3 CUL3 88.9 4.88 13.01 Yes No

IPI00419273.5 Isoform 1 of Cullin-4A CUL4A 87.6 5.01 12.35 Yes No

IPI00965290.1 Isoform 3 of Cullin-4B CUL4B 84 12.75 15.11 Yes No

IPI00101600.5 Isoform 1 of CWF19-like protein 1 CWF19L1 60.6 3.03 1.65 Yes No

IPI00879792.1 Uncharacterized protein CYB5R3 16.7 0 4.69 Yes No

IPI01015263.1 Uncharacterized protein CYBASC3 13.7 0 0 Yes No

IPI00644231.3 Isoform 1 of Cytoplasmic FMR1-interacting protein 1 CYFIP1 145.1 0 14.74 Yes No

IPI00290116.3 Cytochrome P450 11B2, mitochondrial CYP11B2 57.5 1.93 0 Yes No

IPI00926620.1 Protein CYP51A1 25.6 0 2.41 Yes No

IPI00219298.2 Isoform 2 of Disabled homolog 2 DAB2 58.8 0 2.91 Yes No

IPI00982539.1 Dha kinase/FMN cyclase splice variant DAK 54.8 2.21 5.9 Yes No

IPI00984662.1 28S ribosomal protein S29, mitochondrial isoform 3 DAP3 41 0 1.65 Yes No

IPI00216951.2 Aspartyl-tRNA synthetase, cytoplasmic DARS 57.1 16.38 1.63 Yes No

IPI00100460.2 Aspartyl-tRNA synthetase, mitochondrial DARS2 73.5 5.98 6.04 Yes No

IPI00003406.4 Isoform 1 of Drebrin DBN1 71.4 0 14.54 Yes No

IPI00305545.2 Isoform 1 of Lariat debranching enzyme DBR1 61.5 5.46 1.85 Yes No

IPI00020454.1 Deoxycytidine kinase DCK 30.5 0 0 Yes No

IPI00935906.1 dynactin subunit 1 isoform 3 DCTN1 138.7 6.05 12.09 Yes No

IPI01021260.1 Uncharacterized protein DCTN2 15.1 0 1.74 Yes No

IPI00977083.1 Uncharacterized protein DDB1 121.6 14.11 23.04 Yes No

IPI00297084.7


glycosyltransferase 48 kDa subunit DDOST 50.8 0 6.28 Yes No

IPI00889541.2 Isoform 4 of Probable ATP-dependent RNA helicase DDX17 DDX17 80.2 25.57 38.77 Yes No

IPI00155054.3

cDNA FLJ50635, highly similar to ATP-dependent RNA

helicase DDX19A DDX19A 50.4 3.82 9.44 Yes No

IPI00005904.3 Probable ATP-dependent RNA helicase DDX20 DDX20 92.2 0 4.97 Yes No

IPI00477179.1 Isoform 2 of Nucleolar RNA helicase 2 DDX21 79.6 6.53 10.94 Yes No

IPI00903057.1

cDNA FLJ46571 fis, clone THYMU3041428, highly similar to

Probable ATP-dependent RNA helicase DDX23 DDX23 93.2 10.25 6.51 Yes No

IPI00293078.1 Probable ATP-dependent RNA helicase DDX27 DDX27 89.8 1.74 0 Yes No

IPI00829889.1 Isoform 2 of ATP-dependent RNA helicase DDX42 DDX42 90 2.47 9.82 Yes No

IPI00397372.2 probable ATP-dependent RNA helicase DDX47 isoform 2 DDX47 45.1 0 7.51 Yes No

IPI00394982.1 Uncharacterized protein DDX52 55.2 0 2.22 Yes No

IPI00925260.1 Uncharacterized protein DDX56 11.1 0 4.46 Yes No

IPI00942944.1 protein DEK isoform 2 DEK 38.7 0 2.46 Yes No

IPI00386049.4 Isoform 5 of DENN domain-containing protein 4C DENND4C 154.4 0 4.23 Yes No

IPI00029656.1

Isoform Long of Non-syndromic hearing impairment

protein 5 DFNA5 54.5 0 2.13 Yes No

IPI00016703.2 Delta(24)-sterol reductase DHCR24 60.1 0 0 Yes No

IPI00911002.1

cDNA FLJ59986, moderately similar to Deoxyhypusine

synthase DHPS 15.1 0 2.42 Yes No

IPI01015591.2 Uncharacterized protein DHX15 89.4 13.54 28.51 Yes No

IPI00927809.1 Isoform 3 of Putative ATP-dependent RNA helicase DHX30 DHX30 130.5 4.74 8.88 Yes No

IPI00513682.4 probable ATP-dependent RNA helicase DHX35 isoform 2 DHX35 75.4 0 2.15 Yes No

IPI00294211.2

Pre-mRNA-splicing factor ATP-dependent RNA helicase

PRP16 DHX38 140.4 1.77 1.71 Yes No

IPI01009439.1 protein diaphanous homolog 1 isoform 2 DIAPH1 140.2 7.12 13.98 Yes No

IPI00855715.1 Isoform 7 of Protein diaphanous homolog 3 DIAPH3 127.8 4.77 7.54 Yes No

IPI00465045.2 Disco-interacting protein 2 homolog B DIP2B 171.4 3.93 0 Yes No

SI_II(6)


IPI00183462.8 Isoform 2 of Exosome complex exonuclease RRP44 DIS3 105 0 23.3 Yes No

IPI00788836.1 Uncharacterized protein DLAT 57.6 6.18 10.78 Yes No

IPI01011882.1

cDNA FLJ50515, highly similar to Dihydrolipoyl

dehydrogenase, mitochondrial DLD 43.6 2.19 7.11 Yes No

IPI00219919.2 DNA methyltransferase 1-associated protein 1 DMAP1 53 0 1.65 Yes No

IPI00784869.5 Isoform 1 of Dynein heavy chain 10, axonemal DNAH10 514.5 3.44 5.19 Yes No

IPI01019083.1

cDNA FLJ52352, highly similar to DnaJ homolog subfamily

A member 1 DNAJA1 27.5 1.67 11.41 Yes No

IPI00179187.4

Isoform 2 of DnaJ homolog subfamily A member 3,

mitochondrial DNAJA3 49.6 0 2.8 Yes No

IPI00307259.12 DnaJ homolog subfamily C member 13 DNAJC13 254.3 1.83 13.77 Yes No

IPI00455199.3 Isoform 2 of DnaJ homolog subfamily C member 2 DNAJC2 65.9 5.01 2.02 Yes No

IPI00794610.2 dnaJ homolog subfamily C member 7 isoform 2 DNAJC7 50.1 2.55 7.95 Yes No

IPI00936051.2

Isoform 1 of Dynein heavy chain domain-containing

protein 1 DNHD1 533.3 2.19 1.75 Yes No

IPI00657691.2 Isoform 5 of Dynamin-1 DNM1 95.8 17.2 11.29 Yes No

IPI00473085.3 Isoform 3 of Dynamin-1-like protein DNM1L 78.1 4.34 6.39 Yes No

IPI00514550.1 dynamin-2 isoform 2 DNM2 97.9 14.31 11.92 Yes No

IPI00015286.2 Dedicator of cytokinesis protein 1 DOCK1 215.2 0 3.75 Yes No

IPI00816106.3 Isoform 4 of Dedicator of cytokinesis protein 7 DOCK7 238.1 0 1.61 Yes No

IPI00902709.1 diphthine synthase isoform b DPH5 31.6 2.83 0 Yes No

IPI00984387.1

cDNA FLJ53166, highly similar to Dihydropyrimidinase-

related protein 2 DPYSL2 58.1 16.51 10.26 Yes No

IPI00029111.3 dihydropyrimidinase-related protein 3 isoform 1 DPYSL3 73.9 22.57 10.27 Yes No

IPI00031836.3 Developmentally-regulated GTP-binding protein 1 DRG1 40.5 0 2.22 Yes No

IPI00794194.1 Uncharacterized protein DRG2 38.1 0 4.75 Yes No

IPI00028931.2 Desmoglein-2 DSG2 122.2 7.25 16.66 Yes No

IPI00916278.1 Uncharacterized protein DYNC1I2 12.3 0 4.84 Yes No

IPI00007675.6 Cytoplasmic dynein 1 light intermediate chain 1 DYNC1LI1 56.5 6.91 3.03 Yes No

IPI00909090.1

cDNA FLJ52924, highly similar to Cytoplasmic dynein 1

light intermediate chain 2 DYNC1LI2 45 2.25 3.91 Yes No

IPI00788188.3 Isoform 1 of Cytoplasmic dynein 2 heavy chain 1 DYNC2H1 492.3 7.26 11.46 Yes No

IPI00384707.3 EARS2 protein EARS2 59.9 0 2.51 Yes No

IPI00642218.1 Uncharacterized protein EBP 16.7 2.07 1.91 Yes No

IPI00641449.2 Isoform 2 of Enhancer of mRNA-decapping protein 4 EDC4 109.7 0 10.16 Yes No

IPI00329536.2 Early endosome antigen 1 EEA1 162.4 4.92 8.18 Yes No

IPI00014424.1 Elongation factor 1-alpha 2 EEF1A2 50.4 32.96 37.2 Yes No

IPI00980105.1 Uncharacterized protein EEF1D 11.6 3.53 7.17 Yes No

IPI00917777.1

116 kDa U5 small nuclear ribonucleoprotein component

isoform b EFTUD2 105.3 29.24 30.78 Yes No

IPI00924546.1 Uncharacterized protein EGFR 120.6 2.84 5.26 Yes No

IPI01011195.2 Uncharacterized protein EHD2 46.9 1.8 7.02 Yes No

IPI00005578.1 EH domain-containing protein 4 EHD4 61.1 0 3.62 Yes No

IPI00845305.2

cDNA FLJ57962, highly similar to Homo sapiens eukaryotic

translation initiation factor (eIF) 2A (eIF2A), mRNA EIF2A 58 6.05 4.15 Yes No

IPI00385404.2

interferon-induced, double-stranded RNA-activated

protein kinase isoform b EIF2AK2 57.4 0 4.37 Yes No

IPI01011751.2 Uncharacterized protein EIF2B4 57.5 1.99 5.32 Yes No

IPI00219678.3 Eukaryotic translation initiation factor 2 subunit 1 EIF2S1 36.1 5.62 23.22 Yes No

IPI00021728.3 Eukaryotic translation initiation factor 2 subunit 2 EIF2S2 38.4 0 2.27 Yes No

IPI00297982.7 Eukaryotic translation initiation factor 2 subunit 3 EIF2S3 51.1 8.17 7.12 Yes No

IPI00029012.1 Eukaryotic translation initiation factor 3 subunit A EIF3A 166.5 20.8 26.86 Yes No

IPI00016910.1 Eukaryotic translation initiation factor 3 subunit C EIF3C;EIF3CL 105.3 11.8 16.27 Yes No

IPI00984035.1 Uncharacterized protein EIF3E 41.3 2.79 17.96 Yes No

IPI01013988.1


initiation factor 3 subunit 5 EIF3F 33.2 11.56 26.08 Yes No

IPI00977658.1 Eukaryotic translation initiation factor 3 subunit H EIF3H 39.9 0 2.46 Yes No

IPI00910422.2


initiation factor 3subunit 6-interacting protein EIF3L 61 12.48 15.53 Yes No

IPI00102069.5 Eukaryotic translation initiation factor 3 subunit M EIF3M 42.5 1.75 2.64 Yes No

IPI00386533.4

Isoform E of Eukaryotic translation initiation factor 4

gamma 1 EIF4G1 154.7 9.27 22.42 Yes No

IPI00782985.1

Isoform 2 of Eukaryotic translation initiation factor 4

gamma 2 EIF4G2 98.1 4.78 6.59 Yes No

IPI00022648.2 Eukaryotic translation initiation factor 5 EIF5 49.2 0 2.89 Yes No

IPI00299254.4 Eukaryotic translation initiation factor 5B EIF5B 138.7 2.16 5.42 Yes No

IPI00398823.1 Isoform 3 of Zinc phosphodiesterase ELAC protein 2 ELAC2 50.6 2.28 0 Yes No

IPI00940851.1 ELAV-like protein 1 ELAVL1 36.1 4.38 0 Yes No

IPI00640649.1 Uncharacterized protein ELMO2 82.3 2.14 1.81 Yes No

IPI00844099.1 Isoform 2 of Elongator complex protein 3 ELP3 60.6 4.32 0 Yes No

IPI00843958.1

Isoform 2 of Echinoderm microtubule-associated protein-

like 3 EML3 97.9 0 3.6 Yes No

IPI00001466.3 Echinoderm microtubule-associated protein-like 4 EML4 108.8 2.56 6.99 Yes No

IPI00976226.1 Enabled-like protein variant hMenaDv6 ENAH 60.3 0 2.33 Yes No

IPI00922894.1 cDNA FLJ56948, moderately similar to Placental protein 11 ENDOU 17.6 0 3.26 Yes No

IPI00020985.4 Histone acetyltransferase p300 EP300 264 0 5.44 Yes No

IPI00256095.6 Isoform 4 of Band 4.1-like protein 1 EPB41L1 78.8 0 1.84 Yes No

IPI00556351.1 Uncharacterized protein EPB41L2 83.8 2.5 4.35 Yes No

IPI00009896.1 Epoxide hydrolase 1 EPHX1 52.9 2.13 4.31 Yes No

IPI00013452.11 Bifunctional aminoacyl-tRNA synthetase EPRS 170.5 10.13 30.96 Yes No

IPI00640127.1 Uncharacterized protein EPS15 28.5 1.97 0 Yes No

IPI00942431.1 Epidermal growth factor receptor substrate 15-like 1 EPS15L1 94.2 5.68 4.01 Yes No

IPI01015932.1 Uncharacterized protein EPS8 14.5 0 0 Yes No

IPI00414315.10

Isoform 1 of Epidermal growth factor receptor kinase

substrate 8-like protein 2 EPS8L2 80.6 7.64 1.9 Yes No

IPI00216548.4 Isoform HERA-B of GTPase Era, mitochondrial ERAL1 30 2.44 2.33 Yes No

IPI00438291.2 Isoform 6 of Protein LAP2 ERBB2IP 150.5 3.54 1.81 Yes No

IPI00946242.1 Uncharacterized protein ERMP1 93.1 0 0 Yes No

IPI00386755.2 ERO1-like protein alpha ERO1L 54.4 0 3.1 Yes No

IPI00981412.1 Protein ESRP1 56.1 2.05 0 Yes No

IPI00022143.3 Isoform 1 of Extended synaptotagmin-1 ESYT1 122.8 13.51 4.6 Yes No

IPI00827920.1 Isoform 1 of Extended synaptotagmin-2 ESYT2 102.3 7.65 5.54 Yes No

IPI00429191.3 Eukaryotic peptide chain release factor subunit 1 ETF1 49 11.24 17.84 Yes No

IPI00023711.2 Envoplakin EVPL 231.5 3.96 1.85 Yes No

IPI00219954.1 Isoform 2 of Exocyst complex component 3 EXOC3 73.8 0 2.11 Yes No

IPI01015160.2 Uncharacterized protein EXOC4 65.9 0 1.87 Yes No

IPI00719051.4 Exocyst complex component 5 EXOC5 81.8 2.42 0 Yes No

IPI00220336.1 Isoform 2 of Exosome component 10 EXOSC10 98 7 3.22 Yes No

IPI00968203.1 Uncharacterized protein EXOSC9 25.6 0 2.7 Yes No

IPI00070643.6 Isoform Long of FAS-associated factor 1 FAF1 73.9 0 2.87 Yes No

IPI00172656.6 FAS-associated factor 2 FAF2 52.6 0 1.97 Yes No

IPI00794773.1 cDNA FLJ51912, highly similar to Fumarylacetoacetase FAH 19.4 0 0 Yes No

IPI00101734.5 Protein FAM105B FAM105B 40.2 3.03 2.2 Yes No

IPI00039626.3

Isoform D of Constitutive coactivator of PPAR-gamma-like

protein 1 FAM120A 117.3 0 9.23 Yes No

IPI00394751.2

Isoform 1 of Constitutive coactivator of PPAR-gamma-like

protein 2 FAM120C 120.5 0 0 Yes No

IPI00910218.1

cDNA FLJ55143, highly similar to Homo sapiens B-cell

novel protein 1 (BCNP1), mRNA FAM129C 63.4 2.03 1.99 Yes No

IPI00798104.2 Isoform 1 of FTS and Hook-interacting protein FAM160A2 105.5 3.99 3.72 Yes No


IPI00009335.2 Protein FAM203A FAM203A 42.1 1.6 0 Yes No

IPI00953673.1 Isoform 2 of Protein FAM45A FAM45A 39.8 0 0 Yes No

IPI00979101.1 Uncharacterized protein FAM49B 11.1 0 2.75 Yes No

IPI00056537.2 Protein FAM55C FAM55C 63.8 0 1.62 Yes No

IPI00793685.1 Isoform 2 of Protein FAM65A FAM65A 131.8 0 2.05 Yes No

IPI00399119.1 Protein FAM75D1 FAM75D1 175.5 3.64 1.65 Yes No

IPI00979106.1 Uncharacterized protein FAM91A1 88.9 0 0 Yes No

SI_II(7)


IPI00921584.1 Uncharacterized protein FAM98A 34.4 7.75 3.48 Yes No


IPI00604576.1 Isoform 3 of Fanconi anemia group D2 protein FANCD2 140.6 0 2.4 Yes No

IPI00784704.2 Isoform 2 of Fanconi anemia group I protein FANCI 142.3 0 3.52 Yes No

IPI00975671.2 Uncharacterized protein FAR1 38.4 0 3.92 Yes No

IPI01023063.2 Uncharacterized protein FAR2 48.5 0 2.08 Yes No

IPI00909657.1

cDNA FLJ50378, highly similar to Phenylalanyl-tRNA

synthetase alpha chain FARSA 54.1 2.06 2.84 Yes No

IPI00300074.4 Phenylalanyl-tRNA synthetase beta chain FARSB 66.1 5.77 16.69 Yes No

IPI00964536.1 Uncharacterized protein FASTKD3 69.3 0 2 Yes No

IPI00025039.1 rRNA 2'-O-methyltransferase fibrillarin FBL 33.8 1.84 1.94 Yes No

IPI01025801.1 33 kDa protein

FBXO22;FBXO22-

AS1 32.9 0 1.71 Yes No

IPI00914566.2 Farnesyl pyrophosphate synthase FDPS 48.2 10.05 2.54 Yes No

IPI00027776.6 Isoform 1 of Ferrochelatase, mitochondrial FECH 47.8 0 0 Yes No

IPI00795736.2 Isoform 1 of Fer-1-like protein 4 FER1L4 200.9 0 3.36 Yes No

IPI01025386.1 73 kDa protein FERMT2 73.3 2.73 5.94 Yes No

IPI00877625.1 Uncharacterized protein FGG 13.5 1.72 4.07 Yes No

IPI00759715.1

Isoform Cytoplasmic of Fumarate hydratase,

mitochondrial FH 50.2 0 7.09 Yes No

IPI00001730.3 FH1/FH2 domain-containing protein 1 FHOD1 126.5 1.79 4.32 Yes No

IPI00855706.1 Isoform 2 of Fidgetin-like protein 1 FIGNL1 61.6 1.72 9.09 Yes No

IPI00855962.1 Isoform 2 of FK506-binding protein 15 FKBP15 132.4 0 7.8 Yes No

IPI00947340.1 Uncharacterized protein FKBP8 27.5 0 5.45 Yes No

IPI00908582.1

cDNA FLJ50164, highly similar to Protein flightless-1

homolog FLII 135.1 7.32 6.08 Yes No

IPI00302592.2 Isoform 2 of Filamin-A FLNA 279.8 221.78 304.57 Yes No

IPI00900293.1 filamin-B isoform 1 FLNB 281.5 63.45 125.85 Yes No

IPI00789008.1 Flotillin-2 FLOT2 47 2.91 1.96 Yes No

IPI00163516.4 Isoform 1 of Formin-like protein 1 FMNL1 121.8 3.82 8.76 Yes No

IPI00911026.2 Uncharacterized protein FN3KRP 31.4 0 1.63 Yes No

IPI00945904.1 75 kDa protein FNDC3A 74.7 2.69 0 Yes No

IPI01014486.1 Uncharacterized protein FNTA 7.5 0 0 Yes No

IPI01018285.1 cDNA FLJ52799 FOXRED1 35.2 0 0 Yes No

IPI00639911.1 Uncharacterized protein FPGS 29.2 2.86 0 Yes No

IPI00166153.3 Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 FTSJD2 95.3 2.07 2.14 Yes No

IPI00377261.1 Isoform 1 of Far upstream element-binding protein 3 FUBP3 61.6 11.15 17.56 Yes No

IPI00946452.1 Uncharacterized protein FXC1 7.7 0 0 Yes No

IPI00797887.2

cDNA FLJ58199, highly similar to Fragile X mental

retardation syndrome-related protein 1 FXR1 63 0 2.32 Yes No

IPI00012442.1 Ras GTPase-activating protein-binding protein 1 G3BP1 52.1 0 7.38 Yes No

IPI00853547.1 Isoform 3 of Glucose-6-phosphate 1-dehydrogenase G6PD 62.4 4.66 27.78 Yes No

IPI00965736.1 Uncharacterized protein GAK 134.4 0 2.48 Yes No

IPI00641123.1 Uncharacterized protein GALE 21.4 1.63 0 Yes No

IPI00940264.1 Galactokinase GALK1 42.2 4.98 5.68 Yes No

IPI01012012.1 61 kDa protein GALNT2 60.8 0 2.01 Yes No

IPI00011454.1 Isoform 2 of Neutral alpha-glucosidase AB GANAB 109.4 23.36 34.13 Yes No

IPI00946050.1 Uncharacterized protein GAPVD1 157.4 2.03 10.67 Yes No

IPI00783097.4 Glycyl-tRNA synthetase GARS 83.1 7.58 12.92 Yes No

IPI00878204.1 Uncharacterized protein GATSL3 5 0 0 Yes No

IPI00789251.2 Uncharacterized protein GBE1 75.9 1.76 1.99 Yes No

IPI00215768.3 Glutamate--cysteine ligase catalytic subunit GCLC 72.7 0 6.78 Yes No

IPI00001159.11 Translational activator GCN1 GCN1L1 292.6 26.65 46.05 Yes No

IPI00985176.2 cDNA, FLJ78887, highly similar to Guanine deaminase GDA 42.4 3.72 4.71 Yes No

IPI00940148.1 Rab GDP dissociation inhibitor beta GDI2 50.6 4.67 10.19 Yes No

IPI00027717.2 Component of gems 4 GEMIN4 120 0 4.41 Yes No

IPI00291783.4 Gem-associated protein 5 GEMIN5 168.5 1.77 1.76 Yes No

IPI00026321.4

cDNA FLJ12662 fis, clone NT2RM4002205, moderately

similar to ELONGATION FACTOR G, MITOCHONDRIAL GFM1 16.6 5.38 3.21 Yes No

IPI00299506.9

Isoform 2 of Glucosamine--fructose-6-phosphate

aminotransferase [isomerizing] 1 GFPT1 76.7 9.45 4.11 Yes No

IPI01014633.1

cDNA FLJ16322 fis, clone SPLEN2037678, highly similar to

Protein D11Lgp1 homolog GHDC 50.9 1.94 2.12 Yes No

IPI00401971.1 PDZ domain-containing protein GIPC1 isoform 2 GIPC1 26.1 1.98 5.99 Yes No

IPI00795611.1 Isoform 3 of ARF GTPase-activating protein GIT1 GIT1 85.4 0 3.81 Yes No

IPI00030763.2 Gap junction beta-6 protein GJB6 30.4 0 2.23 Yes No

IPI01012645.1

cDNA FLJ10905 fis, clone OVARC1000014, highly similar to

Nucleoporin GLE1 GLE1 50.4 4.03 4.26 Yes No

IPI00008552.6 Glutaredoxin-3 GLRX3 37.4 4.51 15.26 Yes No

IPI00215687.1 Isoform 3 of Glutaminase kidney isoform, mitochondrial GLS 65.4 2.14 0 Yes No

IPI00168262.2 Procollagen galactosyltransferase 1 GLT25D1 71.6 10.12 19.51 Yes No

IPI01014382.2 Uncharacterized protein GLUD1 54.3 5.74 7.54 Yes No

IPI00642048.2 Uncharacterized protein GMDS 39 1.86 0 Yes No

IPI00641457.1 Uncharacterized protein GMEB1 40.2 0 2.13 Yes No

IPI00101782.3

Isoform 1 of Mannose-1-phosphate guanyltransferase

alpha GMPPA 46.3 5.33 0 Yes No

IPI00449197.1 GMP reductase GMPR2 45 0 0 Yes No

IPI01013391.1 cDNA FLJ57604, highly similar to GMP synthase GMPS 71.2 1.81 22.57 Yes No

IPI00220578.3 Guanine nucleotide-binding protein G(k) subunit alpha GNAI3 40.5 9.44 9.9 Yes No

IPI00646491.2

Isoform XLas-2 of Guanine nucleotide-binding protein G(s)

subunit alpha isoforms XLas GNAS 109.6 8.86 12.62 Yes 2.04 4.6 Yes No

IPI00848226.1 Guanine nucleotide-binding protein subunit beta-2-like 1 GNB2L1 35.1 2.02 5.07 Yes No

IPI00982551.1

bifunctional UDP-N-acetylglucosamine 2-epimerase/N-

acetylmannosamine kinase isoform 3 GNE 78.5 0 8.42 Yes No

IPI00843890.1

Isoform 2 of Golgi-associated PDZ and coiled-coil motif-

containing protein GOPC 49.7 0 2.56 Yes No

IPI00018206.4 Aspartate aminotransferase, mitochondrial GOT2 47.5 11.1 6.88 Yes No

IPI00719611.2

Isoform 2 of Glycerol-3-phosphate dehydrogenase,

mitochondrial GPD2 67.5 2.85 4.38 Yes No

IPI01025137.1 30 kDa protein GPHN 30.1 0 1.82 Yes No

IPI00910781.1 glucose-6-phosphate isomerase isoform 1 GPI 64.3 22.99 25.94 Yes No

IPI01010986.1 79 kDa protein GPR110 78.8 0 0 Yes No

IPI00921488.1 Isoform 4 of COP9 signalosome complex subunit 1 GPS1 55.1 0 2.51 Yes No

IPI00965777.1 51 kDa protein GRSF1 51 1.93 0 Yes No

IPI00909083.1

eukaryotic peptide chain release factor GTP-binding

subunit ERF3A isoform 2 GSPT1 68.5 0 4.59 Yes No

IPI00953236.1 glutathione reductase, mitochondrial isoform 2 precursor GSR 53 0 7.83 Yes No

IPI00022820.1 Transcription initiation factor IIB GTF2B 34.8 0 0 Yes No

IPI00945276.1 Uncharacterized protein GTF2E1 14.4 1.85 0 Yes No

IPI00016725.2 General transcription factor 3C polypeptide 4 GTF3C4 91.9 4.67 11.18 Yes No

IPI00514436.1 Uncharacterized protein GTF3C5 52.3 1.72 2.17 Yes No

IPI00644024.3

Isoform 3 of tRNA modification GTPase GTPBP3,

mitochondrial GTPBP3 49.8 1.65 1.72 Yes No

IPI00645138.2

cDNA FLJ55652, highly similar to Nucleolar GTP-binding

protein 1 GTPBP4 68.2 5.11 7.57 Yes No

IPI00927883.1 cDNA FLJ53268, highly similar to Beta-glucuronidase GUSB 58.3 1.63 0 Yes No

IPI00157144.2 glycogen [starch] synthase, muscle isoform 2 GYS1 76.4 2.94 10.07 Yes No

IPI00925802.1

cDNA FLJ54118, highly similar to 2-hydroxyphytanoyl-CoA

lyase HACL1 54.7 0 2.3 Yes No

IPI00031522.2 Trifunctional enzyme subunit alpha, mitochondrial HADHA 82.9 23.07 30.99 Yes No

IPI01018954.1

cDNA FLJ56214, highly similar to Trifunctional enzyme

subunit beta, mitochondrial HADHB 48.8 5.12 5.8 Yes No

IPI00967106.1 Uncharacterized protein HARS 54.8 12.66 8.51 Yes No

IPI00651621.1

Isoform B of Histone acetyltransferase type B catalytic

subunit HAT1 39.8 0 2.26 Yes No

IPI00967058.1 cDNA FLJ52073 HAUS3 56.3 0 2.55 Yes No

IPI00014220.2 Isoform 1 of HAUS augmin-like complex subunit 5 HAUS5 71.6 0 2.92 Yes No

IPI00168609.1 Isoform 2 of HBS1-like protein HBS1L 70.1 0 4.48 Yes No

SI_II(8)


IPI00219326.2 Isoform 2 of Host cell factor 1 HCFC1 201.7 7.81 10.94 Yes No

IPI00289601.10 Histone deacetylase 2 HDAC2 65.5 4.88 6.43 Yes No

IPI00022228.2 Vigilin HDLBP 141.4 8.48 17.34 Yes No

IPI00654611.3 Isoform 2 of HEAT repeat-containing protein 2 HEATR2 92 0 3.88 Yes No

IPI00409555.1 Isoform 3 of HEAT repeat-containing protein 3 HEATR3 35.1 0 2.63 Yes No

IPI01024992.1 162 kDa protein HECTD1 162.1 2.05 8.34 Yes No

IPI00005826.2 E3 ubiquitin-protein ligase HERC2 HERC2 526.9 3.62 5.7 Yes No

IPI00827523.1 Isoform 6 of Probable E3 ubiquitin-protein ligase HERC4 HERC4 106.6 0 0 Yes No

IPI00916785.1

Isoform 2 of Hepatocyte growth factor-regulated tyrosine

kinase substrate HGS 76.3 3.2 2.83 Yes No

IPI00915760.1 Uncharacterized protein HIBCH 30 1.9 3.44 Yes No

IPI00926196.2

cDNA FLJ56396, highly similar to Huntingtin-interacting

protein 1 HIP1 110.6 1.73 3.98 Yes No

IPI00024417.1 Huntingtin-interacting protein 1-related protein HIP1R 119.3 3.85 1.67 Yes No

IPI00217466.3 Histone H1.3 HIST1H1D 22.3 0 5.24 Yes No

IPI00216402.3 Histone H3.1t HIST3H3 15.5 0 1.98 Yes No

IPI01014693.1 cDNA FLJ56506, highly similar to Hexokinase-1 HK1 102.2 4.35 12.26 Yes No

IPI00917193.1 Uncharacterized protein HK2 98.9 1.69 6.33 Yes No

IPI01021784.2 MHC class I antigen (Fragment)

HLA-B;HLA-C;HLA-

A;MICA;LOC441528 34.2 3.39 3.21 Yes No

IPI00472035.1

Isoform 2 of HLA class I histocompatibility antigen, Cw-16

alpha chain

HLA-B;HLA-C;HLA-

A;MICA;LOC441528 36.8 4.29 2.49 Yes No

IPI00963899.3

cDNA FLJ38173 fis, clone FCBBF1000053, highly similar to

HYDROXYMETHYLGLUTARYL-COA SYNTHASE,

CYTOPLASMIC HMGCS1 56.2 6.69 6.06 Yes No

IPI00797148.1 Isoform 2 of Heterogeneous nuclear ribonucleoprotein A1 HNRNPA1 29.4 10.18 18.79 Yes No

IPI00414696.1

Isoform A2 of Heterogeneous nuclear ribonucleoproteins

A2/B1 HNRNPA2B1 36 14.66 31.44 Yes No

IPI00939124.2


ribonucleoprotein A3 HNRNPA3 34.2 3.15 18.33 Yes No

IPI00966854.1 Uncharacterized protein HNRNPAB 30.5 3.16 4.31 Yes No

IPI01024858.1 25 kDa protein HNRNPC 25.2 5.81 14.33 Yes No

IPI00967562.1 Uncharacterized protein HNRNPD 12.6 8.34 6.43 Yes No

IPI01010852.1


ribonucleoprotein F HNRNPF 37.2 1.68 0 Yes No

IPI00908896.2 Uncharacterized protein HNRNPH1 47.1 6.42 19.08 Yes No

IPI00807545.1 Isoform 3 of Heterogeneous nuclear ribonucleoprotein K HNRNPK 48.5 23.69 40.02 Yes No

IPI00383296.5 Isoform 2 of Heterogeneous nuclear ribonucleoprotein M HNRNPM 73.6 33.07 38.04 Yes No

IPI00012074.3 Isoform 1 of Heterogeneous nuclear ribonucleoprotein R HNRNPR 70.9 15.62 16.05 Yes No

IPI00736859.1

Isoform 4 of Heterogeneous nuclear ribonucleoprotein U-

like protein 1 HNRNPUL1 84.7 0 8.82 Yes No

IPI00456887.2 Heterogeneous nuclear ribonucleoprotein U-like protein 2 HNRNPUL2 85.1 3.04 9.29 Yes No

IPI00045498.4

Isoform 3 of Heterogeneous nuclear ribonucleoprotein D-

like HNRPDL 27.2 6.04 5.19 Yes No

IPI00646486.1 Isoform 2 of Heterochromatin protein 1-binding protein 3 HP1BP3 57.2 1.7 5.32 Yes No

IPI00063762.1 4-hydroxyphenylpyruvate dioxygenase-like protein HPDL 39.4 0 2.21 Yes No

IPI00015505.7 Hermansky-Pudlak syndrome 6 protein HPS6 82.9 0 3.65 Yes No

IPI00940185.1 heparan sulfate glucosamine 3-O-sulfotransferase 6 HS3ST6 34.7 3.48 2.08 Yes No

IPI01013542.1 Uncharacterized protein HSD17B4 53.9 5.99 5.07 Yes No

IPI00171459.4 Inactive hydroxysteroid dehydrogenase-like protein 1 HSDL1 37 1.8 0 Yes No

IPI00031107.1 Isoform 2 of Hydroxysteroid dehydrogenase-like protein 2 HSDL2 37.3 0 3.6 Yes No

IPI00555957.1 Putative heat shock protein HSP 90-alpha A4 HSP90AA4P 47.7 19.35 22.06 Yes No

IPI00299299.3 Heat shock 70 kDa protein 13 HSPA13 51.9 0 0 Yes No

IPI00292499.4 Heat shock 70 kDa protein 14 HSPA14 54.8 0 0 Yes No

IPI00964409.3 Uncharacterized protein HSPA4L 91.9 4.21 8.94 Yes No

IPI00100748.4 cDNA FLJ55382, highly similar to Hsp70-binding protein 1 HSPBP1 44.5 0 0 Yes No

IPI00784154.1 60 kDa heat shock protein, mitochondrial HSPD1 61 49.3 60.95 Yes No

IPI00218993.1 Isoform Beta of Heat shock protein 105 kDa HSPH1 92.1 20 14.5 Yes No

IPI00644220.4

cDNA FLJ60139, highly similar to Homo sapiens HIV TAT

specific factor 1 (HTATSF1), mRNA HTATSF1 82 0 0 Yes No

IPI00902529.1

cDNA FLJ34625 fis, clone KIDNE2015244, highly similar to

Serine protease HTRA1 HTRA1 48.4 0 2.3 Yes No

IPI00002335.1 huntingtin HTT 347.6 1.71 6.31 Yes No

IPI00445401.4 Isoform 2 of E3 ubiquitin-protein ligase HUWE1 HUWE1 479.9 7.06 27.42 Yes No

IPI00922838.1

cDNA FLJ56074, highly similar to 150 kDa oxygen-

regulated protein HYOU1 109.6 6.64 9.15 Yes No

IPI00017283.2 Isoleucyl-tRNA synthetase, mitochondrial IARS2 113.7 7.21 14.57 Yes No

IPI00220373.5 Insulin-degrading enzyme IDE 117.9 3.13 8.65 Yes No

IPI01015385.1 Isocitrate dehydrogenase IDH2 45.2 2.54 3.93 Yes No

IPI00030702.1

Isoform 1 of Isocitrate dehydrogenase [NAD] subunit

alpha, mitochondrial IDH3A 39.6 5.57 5.66 Yes No

IPI00304417.7

Isocitrate dehydrogenase [NAD] subunit beta,

mitochondrial precursor IDH3B 42.9 12.22 14.06 Yes No

IPI00908370.3 Uncharacterized protein IFT122 118.4 0 5.62 Yes No

IPI00027232.3 Insulin-like growth factor 1 receptor IGF1R 154.7 0 0 Yes No

IPI00008557.6 Insulin-like growth factor 2 mRNA-binding protein 1 IGF2BP1 63.4 3.41 7.3 Yes No

IPI00658000.3

Isoform 1 of Insulin-like growth factor 2 mRNA-binding

protein 3 IGF2BP3 63.7 5.15 4.87 Yes No

IPI00289819.5 Cation-independent mannose-6-phosphate receptor IGF2R 274.2 6.58 9.07 Yes No

IPI00966544.1 Uncharacterized protein IK 24.7 0 1.99 Yes No

IPI00797136.2

Isoform 1 of Inhibitor of nuclear factor kappa-B kinase-

interacting protein IKBIP 39.3 6.7 0 Yes No

IPI00981273.2 Uncharacterized protein IKBKAP 111.4 5.51 2.61 Yes No

IPI00981929.2

cDNA FLJ33978 fis, clone DFNES2004354, highly similar to

Inhibitor of nuclear factor kappa B kinase subunit beta IKBKB 60.8 0 5.94 Yes No

IPI00554522.1 Uncharacterized protein IL6ST 23 3.49 3.51 Yes No

IPI00219330.2 Isoform 5 of Interleukin enhancer-binding factor 3 ILF3 74.6 18.19 29.24 Yes No

IPI01012931.1

cDNA FLJ53825, highly similar to Integrin-linked protein

kinase 1 ILK 36.4 3.9 0 Yes No

IPI00556135.1

IlvB (Bacterial acetolactate synthase)-like isoform 1 variant

(Fragment) ILVBL 45.6 3.63 3.55 Yes No

IPI00910079.3

cDNA FLJ59418, highly similar to Mitochondrial inner

membrane protein IMMT 73.1 8.51 7.44 Yes No

IPI00945952.1 Uncharacterized protein IMPDH1 19.5 0 1.76 Yes No

IPI00291510.3 Inosine-5'-monophosphate dehydrogenase 2 IMPDH2 55.8 9.8 27.54 Yes No

IPI00895800.3 Isoform 1 of Inverted formin-2 INF2 135.5 0 1.8 Yes No

IPI00847557.2

Isoform 3 of Phosphatidylinositol-3,4,5-trisphosphate 5-

phosphatase 1 INPP5D;LOC646743 109.2 1.66 5.12 Yes No

IPI00016932.4

Isoform 1 of Phosphatidylinositol-3,4,5-trisphosphate 5-

phosphatase 2 INPPL1 138.5 0 9.29 Yes No

IPI00740961.5 DKFZP586J0619 protein INTS1 266.5 0 2.7 Yes No

IPI00418336.3 Isoform 2 of Integrator complex subunit 3 INTS3 117.9 1.85 2.36 Yes No

IPI00301107.7 Isoform 1 of Importin-11 IPO11 112.5 0 5.31 Yes No

IPI00156374.6 Isoform 1 of Importin-4 IPO4 118.6 2.91 4.86 Yes No

IPI00007401.2 Importin-8 IPO8 119.9 1.93 7.35 Yes No

IPI00185146.5 Importin-9 IPO9 115.9 0 9.61 Yes No

IPI00968174.1 Uncharacterized protein IQGAP2 160.2 4.82 0 Yes No

IPI00019205.1 Interferon regulatory factor 2-binding protein-like IRF2BPL 82.6 1.85 3.23 Yes No

IPI00464981.2 Uncharacterized protein ITFG3 14.9 0 2.44 Yes No

IPI00220847.2 Isoform Beta-4D of Integrin beta-4 ITGB4 194.3 0 5.61 Yes No

IPI00218660.3 Isoform 4 of Inositol 1,4,5-trisphosphate receptor type 1 ITPR1 306.6 6.79 14.85 Yes No

IPI00291607.2 Inositol 1,4,5-trisphosphate receptor type 3 ITPR3 303.9 2.04 7.97 Yes No

IPI00929108.1

isovaleryl-CoA dehydrogenase, mitochondrial isoform 2

precursor IVD 43 0 2.88 Yes No

IPI00013439.3 Transcription factor jun-B JUNB 35.9 3.97 0 Yes No

IPI00011174.2 Anosmin-1 KAL1 76.1 2.11 3.12 Yes No

IPI00307092.2 Isoform Mitochondrial of Lysyl-tRNA synthetase KARS 71.5 15.89 30.55 Yes No

IPI00060715.1 BTB/POZ domain-containing protein KCTD12 KCTD12 35.7 2.74 3.34 Yes No

SI_II(9)


IPI00456631.5 Isoform 1 of Lysine-specific histone demethylase 1A KDM1A 92.8 2.16 8.25 Yes No

IPI00748132.2 Uncharacterized protein KDM3B 88.4 0 3.06 Yes No

IPI00385834.3

Isoform 2 of KH domain-containing, RNA-binding, signal

transduction-associated protein 1 KHDRBS1 45.8 0 4.27 Yes No

IPI00791325.2 Pumilio domain-containing protein KIAA0020 KIAA0020 73.5 1.96 3.92 Yes No

IPI00158296.6 Uncharacterized protein KIAA0564 204.1 6.44 4.06 Yes No

IPI00939707.1 Protein KIAA0664 KIAA0664 146.6 8.31 11.07 Yes No

IPI00164930.5 Isoform 2 of WASH complex subunit 7 KIAA1033 98.7 0 4.53 Yes No

IPI00036742.6 Isoform 1 of Protein virilizer homolog KIAA1429 201.9 0 3.42 Yes No

IPI00854856.3 Isoform 4 of Shootin-1 KIAA1598 63.5 1.97 3.68 Yes No

IPI00748360.2 Uncharacterized protein KIAA1797 KIAA1797 199.9 0 2.54 Yes No

IPI00152853.3 Isoform 1 of Phostensin KIAA1949 67.9 4.04 6.32 Yes No

IPI00305289.2 Kinesin-like protein KIF11 KIF11 119.1 0 8.07 Yes No

IPI00062047.5 Kinesin-like protein KIF12 KIF12 70.6 1.74 0 Yes No

IPI00974535.1 Uncharacterized protein KIF15 120.3 0 10.56 Yes No

IPI00216252.5 Isoform 2 of Kinesin-like protein KIF1B KIF1B 199.1 3.92 8.86 Yes No

IPI00968120.1

cDNA FLJ55710, highly similar to Kinesin family member

20A KIF20A 98 0 1.85 Yes No

IPI00293884.6 Isoform 2 of Kinesin-like protein KIF23 KIF23 98 0 3.56 Yes No

IPI00867529.1 Isoform 2 of Kinesin-like protein KIF2A KIF2A 75 3.38 6.26 Yes No

IPI00552801.2 cDNA FLJ54088, highly similar to Kinesin-like protein KIF2C KIF2C 76.9 0 2.38 Yes No

IPI00556059.1 Isoform 2 of Chromosome-associated kinesin KIF4A KIF4A 128.4 2.01 7.58 Yes No

IPI00012837.1 Kinesin-1 heavy chain KIF5B 109.6 27 29.25 Yes No

IPI00879869.1 Uncharacterized protein KLHL22 33.8 0 0 Yes No

IPI00001458.1 Kinetochore-associated protein 1 KNTC1 250.6 4.19 1.96 Yes No

IPI00002214.1 Importin subunit alpha-2 KPNA2 57.8 8.36 13.27 Yes No

IPI00299033.1 Importin subunit alpha-3 KPNA3 57.8 0 1.87 Yes No

IPI01012685.1 Uncharacterized protein KPNA6 59.6 5.55 6.96 Yes No

IPI00909030.1 cDNA FLJ61494, highly similar to Kinectin KTN1 152.9 11.43 16.7 Yes No

IPI00003818.1 Kynureninase KYNU 52.3 1.67 5.86 Yes No

IPI00980890.2

cDNA FLJ58780, highly similar to Homo sapiens lysosomal-

associated membrane protein 2 (LAMP2), transcript

variant LAMP2B, mRNA LAMP2 39.8 0 2.34 Yes No

IPI00924801.1 Uncharacterized protein LANCL1 9.3 0 3.18 Yes No

IPI00032995.1 LanC-like protein 2 LANCL2 50.8 0 1.94 Yes No

IPI00963851.1 23 kDa protein LAP3 22.6 5.47 5.36 Yes No

IPI00411690.1 Isoform 2 of La-related protein 1 LARP1 116.4 14.87 14.87 Yes No

IPI00953461.1 La-related protein 4B LARP4B 80.5 0 1.89 Yes No

IPI00909939.1

cDNA FLJ52195, highly similar to LIM and SH3 domain

protein 1 LASP1 14.6 4.76 0 Yes No

IPI00010471.6 Plastin-2 LCP1 70.2 3.68 13.95 Yes No

IPI00217966.9 Isoform 1 of L-lactate dehydrogenase A chain LDHA 36.7 25.93 33.84 Yes No

IPI00219217.3 L-lactate dehydrogenase B chain LDHB 36.6 15.11 17.69 Yes No

IPI00017592.1

Isoform 1 of LETM1 and EF-hand domain-containing

protein 1, mitochondrial LETM1 83.3 2.14 2.04 Yes No

IPI01010750.1 DNA ligase (Fragment) LIG1 91.9 5.96 7.88 Yes No

IPI00000156.3 Isoform Beta of DNA ligase 3 LIG3 106 2.17 4.77 Yes No

IPI00008918.1 Isoform Beta of LIM domain and actin-binding protein 1 LIMA1 85.2 1.92 9.76 Yes No

IPI00788612.2

LIM and senescent cell antigen-like-containing domain

protein 1 LIMS1 37.2 5.56 0 Yes No

IPI00479478.1 Putative uncharacterized protein CXorf52B LINC00204B 13.8 0 0 Yes No

IPI00854741.2 Lethal(2) giant larvae protein homolog 1 LLGL1 115.3 0 3.44 Yes No

IPI00966738.1 Uncharacterized protein LMAN2 23 0 0 Yes No

IPI00216953.1 Isoform ADelta10 of Prelamin-A/C LMNA 70.6 26.62 49.24 Yes No

IPI00217975.4 Lamin-B1 LMNB1 66.4 11.21 9.04 Yes No

IPI00221241.3 Isoform 3 of Leucyl-cystinyl aminopeptidase LNPEP 115 0 4.28 Yes No

IPI00444708.3 Conserved hypothetical protein LOC100131662 22.6 0 0 Yes No

IPI00976527.1 Conserved hypothetical protein LOC100288306 10.8 0 0 Yes No

IPI00983324.1 protein FAM115A-like LOC100508781 53.6 5.59 6.97 Yes No

IPI00419792.3

Similar to Dual specificity mitogen-activated protein

kinase kinase 2 LOC407835 42.5 0 2.01 Yes No

IPI00978330.1 Conserved hypothetical protein LOC646903 14.3 1.83 0 Yes No

IPI00334291.9 Lon protease homolog LONP1 103 6.28 11.81 Yes No

IPI00171626.3 Lysophosphatidylcholine acyltransferase 1 LPCAT1 59.1 2.44 0 Yes No

IPI00926532.1 Uncharacterized protein LPP 26.7 3.06 0 Yes No

IPI00967340.1 Uncharacterized protein LRBA 286.8 6.93 9.56 Yes No

IPI00152998.3 Leucine-rich repeat-containing protein 40 LRRC40 68.2 0 4.35 Yes No

IPI00170935.1 Leucine-rich repeat-containing protein 47 LRRC47 63.4 3.78 15.79 Yes No

IPI00396321.1 Leucine-rich repeat-containing protein 59 LRRC59 34.9 2.74 2.55 Yes No

IPI00887636.1

Isoform 1 of Leucine-rich repeat and death domain-

containing protein 1 LRRD1 98 0 2.04 Yes No

IPI00023576.3 Leucine-rich repeat transmembrane neuronal protein 2 LRRTM2 59 0 0 Yes No

IPI00069309.6 Leucine-rich repeat and WD repeat-containing protein 1 LRWD1 70.8 3.46 0 Yes No

IPI00923487.1 lanosterol synthase isoform 3 LSS 74.2 0 7.13 Yes No

IPI00790203.2 Uncharacterized protein LTA4H 66.8 6.71 13.07 Yes No

IPI00783835.2 E3 ubiquitin-protein ligase listerin LTN1 205 1.74 2.22 Yes No

IPI00298625.2 Isoform LYN A of Tyrosine-protein kinase Lyn LYN 58.5 9.42 10.91 Yes No

IPI00550385.3

Isoform 1 of Microtubule-actin cross-linking factor 1,

isoforms 1/2/3/5 MACF1 837.8 5.9 6.5 Yes No

IPI00910834.1

cDNA FLJ54833, moderately similar to Homo sapiens

macrophage erythroblast attacher (MAEA), transcript

variant 1, mRNA MAEA 36.4 0 2.12 Yes No

IPI00009542.1 Isoform 1 of Melanoma-associated antigen D2 MAGED2 64.9 0 0 Yes No

IPI00008868.4 Microtubule-associated protein 1B MAP1B 270.5 7.55 12.7 Yes No

IPI01014928.1

cDNA FLJ55328, highly similar to Homo sapiens BPY2

interacting protein 1 (BPY2IP1), mRNA MAP1S 109.7 3.62 1.62 Yes No

IPI01015278.1

cDNA FLJ58314, highly similar to Mitogen-activated

protein kinase 1 MAPK1 40.4 3.93 4.31 Yes No

IPI00003145.1 Isoform 1 of Mitogen-activated protein kinase 8 MAPK8 44.2 2.58 7.04 Yes No

IPI00026054.1 Isoform 1 of MAP kinase-activated protein kinase 2 MAPKAPK2 45.5 3.69 4.08 Yes No

IPI00219301.7 Myristoylated alanine-rich C-kinase substrate MARCKS 31.5 0 3.68 Yes No

IPI00008240.2 Methionyl-tRNA synthetase, cytoplasmic MARS 101.1 6.04 19.27 Yes No

IPI00010157.1 S-adenosylmethionine synthase isoform type-2 MAT2A 43.6 12.96 9.55 Yes No

IPI00845510.1

Isoform 4 of Methionine adenosyltransferase 2 subunit

beta MAT2B 34.6 8.37 9.69 Yes No

IPI00789551.1 Uncharacterized protein MATR3;SNHG4 99.9 6.97 31.45 Yes No

IPI00935784.2 R29828_1 (Fragment) MAU2 58.3 1.86 1.69 Yes No

IPI00023359.1

Isoform 1 of Malonyl-CoA-acyl carrier protein

transacylase, mitochondrial MCAT 42.9 0 4.32 Yes No

IPI00792499.1 Uncharacterized protein MCCC1 68.3 9.62 13.4 Yes No

IPI00789428.1

Isoform 2 of Methylcrotonoyl-CoA carboxylase beta chain,

mitochondrial MCCC2 57.5 2.34 2.12 Yes No

IPI01013402.2 Uncharacterized protein MCM2 91.2 28.18 28.72 Yes No

IPI00028954.1 80 kDa MCM3-associated protein MCM3AP 218.3 3.53 10.6 Yes No

IPI00795318.2

cDNA FLJ54365, highly similar to DNA replication licensing

factor MCM4 MCM4 92.7 5.93 7.23 Yes No



IPI00855969.1 Isoform 2 of DNA replication licensing factor MCM9 MCM9 44 0 0 Yes No

IPI00915869.3 malate dehydrogenase, cytoplasmic isoform 3 MDH1 27 4.14 5.39 Yes No

IPI00927864.2 25 kDa protein MDH2 24.6 3.71 11.52 Yes No

IPI00167941.1 Midasin MDN1 632.4 11.25 8.27 Yes No

IPI00008215.1 NADP-dependent malic enzyme ME1 64.1 0 4.41 Yes No

IPI00643577.1

NAD-dependent malic enzyme, mitochondrial isoform 2

precursor ME2 53.6 0 1.92 Yes No

IPI00297191.2 Mediator of RNA polymerase II transcription subunit 14 MED14 160.5 0 1.64 Yes No

IPI01008916.1 Uncharacterized protein (Fragment) MED16 58.8 2.48 2.46 Yes No

SI_II(10)


IPI00294682.2

Isoform 1 of Mediator of RNA polymerase II transcription

subunit 26 MED26 65.4 0 0 Yes No

IPI01022532.1 Uncharacterized protein METAP2 48.8 0 4.36 Yes No

IPI00409639.1 Isoform 4 of Methyltransferase-like protein 13 METTL13 61.1 2 4.07 Yes No

IPI00921997.2 Uncharacterized protein METTL16 22.1 0 3.09 Yes No

IPI00181391.9 bifunctional protein NCOAT isoform b MGEA5 96.9 3.35 4.57 Yes No

IPI00975687.1 midline-1 isoform 6 MID1 25.6 0 3.98 Yes No

IPI00395527.2 Isoform 4 of MYC-induced nuclear antigen MINA 52.6 3.35 0 Yes No

IPI00889558.1 Isoform 2 of WD repeat-containing protein mio MIOS 64 2.07 2.25 Yes No

IPI01025254.1

Isoform 3 of Cleavage and polyadenylation specificity

factor subunit 6 MIR1279;CPSF6 52.3 6.22 6.03 Yes No

IPI00552413.2 Isoform 2 of Cyclin-dependent kinase 9 MIR2861;CDK9 53.3 7.33 8.46 Yes No

IPI00413173.3 Isoform Short of Antigen KI-67 MKI67 319.2 6.67 6.54 Yes No

IPI00180781.4 Isoform 1 of Mixed lineage kinase domain-like protein MLKL 54.4 2.77 4.48 Yes No

IPI00297859.2 Isoform 1 of Histone-lysine N-methyltransferase MLL2 MLL2 593 0 3.43 Yes No

IPI00916630.1 Protein MLPH 25.9 0 1.65 Yes No

IPI00964041.1 Uncharacterized protein MMS22L 137.4 0 1.92 Yes No

IPI01021118.1 Uncharacterized protein MON2 185.7 3.1 10.8 Yes No

IPI00793583.2 Uncharacterized protein MORC1 110.5 1.9 1.74 Yes No

IPI00169283.1 Isoform 1 of Motile sperm domain-containing protein 2 MOSPD2 59.7 2.2 0 Yes No

IPI00916455.1 Uncharacterized protein MPP6 48.7 2.01 3.55 Yes No

IPI00305344.7 Isoform 1 of Myosin phosphatase Rho-interacting protein MPRIP 116.5 5.68 3.59 Yes No

IPI00940798.1 Isoform 1 of Double-strand break repair protein MRE11A MRE11A 80.5 2.32 8.1 Yes No

IPI00783656.1 39S ribosomal protein L38, mitochondrial MRPL38 44.6 0 1.63 Yes No

IPI00009680.3 39S ribosomal protein L44, mitochondrial MRPL44 37.5 0 0 Yes No

IPI00789525.2

cDNA PSEC0127 fis, clone PLACE1003724, highly similar to

Mitochondrial 28S ribosomal protein S22 MRPS22 25.1 7.05 4.62 Yes No

IPI00006440.7 28S ribosomal protein S7, mitochondrial MRPS7 28.1 0 1.68 Yes No

IPI01014700.1

cDNA FLJ51193, highly similar to DNA mismatch repair

protein Msh2 MSH2 97.3 3.63 8.98 Yes No


MSH5-

C6orf26;MSH5 18.4 0 0 Yes No

IPI01014931.1

cDNA FLJ55677, highly similar to DNA mismatch repair

protein MSH6 MSH6 137.9 4.08 9.41 Yes No

IPI00856061.2

cDNA FLJ54699, highly similar to Homo sapiens misato

homolog 1 (MSTO1), mRNA MSTO1 54.7 1.92 4.66 Yes No

IPI00879166.1 Uncharacterized protein MTA1 79.1 0 7.66 Yes No

IPI00171798.1 Metastasis-associated protein MTA2 MTA2 75 2.45 12.22 Yes No

IPI00291646.3

Methylenetetrahydrofolate dehydrogenase (NADP+

dependent) 1-like MTHFD1L 105.8 5.87 17.82 Yes No

IPI00917592.1 Uncharacterized protein MTMR2 64.5 0 8.2 Yes No

IPI00008495.5 NADH-ubiquinone oxidoreductase chain 4 MT-ND4 51.7 0 0 Yes No

IPI00031410.1 Serine/threonine-protein kinase mTOR MTOR 288.7 0 12.38 Yes No

IPI00470416.2 Isoform 2 of Poly(A) RNA polymerase, mitochondrial MTPAP 78.8 0 2.97 Yes No

IPI00909023.1 cDNA FLJ56487, highly similar to Methionine synthase MTR 124 4.23 8.46 Yes No

IPI00024934.5 Methylmalonyl-CoA mutase, mitochondrial MUT 83.1 1.81 12.24 Yes No

IPI00910019.3 Uncharacterized protein MVP 87.6 5.63 3.39 Yes No

IPI00005024.3 Isoform 1 of Myb-binding protein 1A MYBBP1A 148.8 0 4.24 Yes No

IPI00397526.3 Isoform 1 of Myosin-10 MYH10 228.9 14.46 26.37 Yes No

IPI00298301.4 Myosin-3 MYH3 223.8 1.92 8.24 Yes No

IPI00742780.1 FLJ00279 protein (Fragment) MYH9 65.7 42.16 53.01 Yes 0 3.05 Yes No

IPI00940399.2 Uncharacterized protein MYO10 163.5 1.81 4.84 Yes No

IPI01014006.1 Isoform 5 of Myosin-XVIIIa MYO18A 180.4 3.63 1.75 Yes No

IPI00376344.3 Isoform 1 of Myosin-Ib MYO1B 131.9 2.84 9.51 Yes No

IPI00010418.6 Isoform 2 of Myosin-Ic MYO1C 117.8 4.37 31.69 Yes No

IPI00329719.1 Myosin-Id MYO1D 116.1 0 3.58 Yes No

IPI00329672.4 Myosin-Ie MYO1E 127 4.63 5.2 Yes No

IPI00420004.4 Isoform 1 of Myosin-Ig MYO1G 116.4 1.68 2.07 Yes No

IPI00479962.4 Myosin-Vb MYO5B 213.6 2.02 6.02 Yes No

IPI00642722.2 Isoform 5 of Myosin-VI MYO6 145 0 3.24 Yes No

IPI00902943.2 Isoform 5 of Myosin-IXa MYO9A 265.2 4.24 1.62 Yes No

IPI00216268.1 Isoform 2 of Myoferlin MYOF 229.7 28.25 60.44 Yes No

IPI00032158.3

Isoform 2 of N-alpha-acetyltransferase 15, NatA auxiliary

subunit NAA15 101.1 13.44 26.44 Yes No

IPI00852843.1

Isoform 2 of N-alpha-acetyltransferase 25, NatB auxiliary

subunit NAA25 99.2 0 4.22 Yes No

IPI00654793.2 Isoform 3 of NAD kinase domain-containing protein 1 NADKD1 31.7 4.46 8.75 Yes No

IPI00604652.1 NEDD8-activating enzyme E1 regulatory subunit isoform c NAE1 50.6 8.89 3.39 Yes No

IPI00018873.1 Nicotinamide phosphoribosyltransferase NAMPT 55.5 1.72 6.18 Yes No

IPI00147874.1 Sialic acid synthase NANS 40.3 3.39 5.45 Yes No

IPI01022667.1 Uncharacterized protein NAP1L1 18.8 0 5.95 Yes No

IPI00984634.1 Uncharacterized protein NAP1L4 12.2 0 5.76 Yes No

IPI00880164.1 Isoform 3 of Nicotinate phosphoribosyltransferase NAPRT1 56.1 3.56 1.86 Yes No

IPI00306960.3 Asparaginyl-tRNA synthetase, cytoplasmic NARS 62.9 8.65 17.25 Yes No

IPI00982972.1 Protein NARS2 12 0 2.61 Yes No

IPI00179953.2 Isoform 1 of Nuclear autoantigenic sperm protein NASP 85.2 6.09 5.62 Yes No

IPI00300127.4 N-acetyltransferase 10 NAT10 115.7 3.93 0 Yes No

IPI00333913.9 Isoform 1 of Neuroblastoma-amplified sequence NBAS 268.4 0 0 Yes No

IPI01014221.2 Condensin complex subunit 1 NCAPD2 152.1 5.79 0 Yes No

IPI00106495.1 Condensin complex subunit 3 NCAPG 114.3 0 2.36 Yes No

IPI00871611.2 Uncharacterized protein NCAPG2 101.7 0 2.19 Yes No

IPI00019380.1 Nuclear cap-binding protein subunit 1 NCBP1 91.8 3.98 6.25 Yes No

IPI00031982.1 Isoform 1 of Nck-associated protein 1 NCKAP1 128.7 0 9.25 Yes No

IPI00419253.5 Isoform 1 of Nck-associated protein 5 NCKAP5 208.4 1.88 3.56 Yes No

IPI00983197.1 Uncharacterized protein NCOA2 102.2 0 2.23 Yes No

IPI00923531.1 Isoform 5 of Nuclear receptor corepressor 2 NCOR2 268.2 0 6.37 Yes No

IPI01014309.1 cDNA FLJ54521, highly similar to Protein NDRG3 NDRG3 29.7 1.85 2.18 Yes No

IPI00922477.2

cDNA FLJ59302, highly similar to Bifunctional heparan

sulfate N-deacetylase/N-sulfotransferase 1 NDST1 94.3 2.17 0 Yes No

IPI00925023.9

NADH-ubiquinone oxidoreductase 75 kDa subunit,

mitochondrial isoform 4 NDUFS1 73.5 2.24 8.2 Yes No

IPI00946334.1

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2,

mitochondrial isoform 2 precursor NDUFS2 51.8 5.52 0 Yes No

IPI00028520.2

Isoform 1 of NADH dehydrogenase [ubiquinone]

flavoprotein 1, mitochondrial NDUFV1 50.8 2.32 4.5 Yes No

IPI00301609.8 Serine/threonine-protein kinase Nek9 NEK9 107.1 2.62 4.72 Yes No

IPI01026321.1 114 kDa protein NEMF 113.9 1.61 4.89 Yes No

IPI00010800.2 Nestin NES 177.3 2.68 3.3 Yes No

IPI01010746.1 Uncharacterized protein NGEF 69.7 1.74 4.34 Yes No

IPI00301051.3 Isoform 1 of NHL repeat-containing protein 2 NHLRC2 79.4 1.63 4.35 Yes No

IPI00414367.4 Isoform 2 of NHS-like protein 1 NHSL1 170.4 1.97 1.72 Yes No

IPI00971005.1 Isoform 8 of Ninein NIN 238.8 1.92 4.1 Yes No

IPI00872224.1

cDNA FLJ14696 fis, clone NT2RP2005775, highly similar to

NEUROLYSIN NLN 43.1 6.38 7.51 Yes No

IPI00790856.2 Nucleoside diphosphate kinase

NME1;NME2;NME1

-NME2 8.7 0 2.11 Yes No

IPI00337541.3 NAD(P) transhydrogenase, mitochondrial NNT 113.8 8.8 16.57 Yes No

IPI00291903.4 Isoform 3 of Nucleolar protein 6 NOL6 77.6 0 1.93 Yes No

IPI01009730.1 Uncharacterized protein NOMO3;NOMO1 122 2.47 12.98 Yes No

IPI00922367.1

non-POU domain-containing octamer-binding protein

isoform 2 NONO 43.8 4.87 3.55 Yes No

IPI00220339.3 Isoform 2 of Nitric oxide synthase, inducible NOS2 126.7 1.8 0 Yes No

IPI00978387.1 Uncharacterized protein NPEPPS 102.9 7.49 17.57 Yes No

IPI01013928.1

cDNA FLJ57969, highly similar to Nuclear protein

localization protein 4 homolog NPLOC4 51.5 0 1.84 Yes No

IPI00027200.1 Atrial natriuretic peptide receptor 1 NPR1 118.8 0 1.7 Yes No

IPI00789037.1 COUP transcription factor 2 isoform b NR2F2 31.5 2.15 1.93 Yes No

IPI00759729.2 Isoform Beta-B of Glucocorticoid receptor NR3C1 78.6 0 3.97 Yes No

SI_II(11)


IPI01014543.1

cDNA FLJ54186, highly similar to Nuclear receptor-binding

protein NRBP1 57.9 0 3.53 Yes No

IPI01012543.1 Uncharacterized protein NRD1 131.1 1.72 10.05 Yes No

IPI00219917.1 Isoform Short of Nuclear respiratory factor 1 NRF1 46.1 0 2.55 Yes No

IPI00985110.2 cDNA FLJ59316, highly similar to Vesicle-fusing ATPase NSF 71.5 0 22.23 Yes No

IPI00306369.3 tRNA (cytosine(34)-C(5))-methyltransferase NSUN2 86.4 7.26 5.62 Yes No

IPI00797435.2 23 kDa protein NT5DC2 23.4 2.32 4.46 Yes No

IPI00006193.1 Netrin-3 NTN3 61.4 0 0 Yes No

IPI00869040.2 Isoform 2 of Cytosolic Fe-S cluster assembly factor NUBP1 NUBP1 33.4 1.86 0 Yes No

IPI00550746.4 Nuclear migration protein nudC NUDC 38.2 2.57 4.91 Yes No

IPI00789225.3 Isoform 3 of NudC domain-containing protein 1 NUDCD1 56.6 2.2 5.67 Yes No

IPI00006196.3 Isoform 2 of Nuclear mitotic apparatus protein 1 NUMA1 236.4 9.18 17.28 Yes No

IPI00028005.1 Nuclear pore complex protein Nup107 NUP107 106.3 5.36 6.62 Yes No

IPI01015229.1 Uncharacterized protein NUP133 127.3 3.24 4.81 Yes No


IPI00965680.1 Uncharacterized protein NUP155 148 3.53 13.74 Yes No

IPI00748807.2 Isoform 1 of Nuclear pore complex protein Nup160 NUP160 162 8.45 11.57 Yes No

IPI00291755.6 Isoform 1 of Nuclear pore membrane glycoprotein 210 NUP210 205 4.92 5.38 Yes No

IPI00900318.1 Isoform 2 of Nuclear pore complex protein Nup214 NUP214 212.4 2.76 2.66 Yes No

IPI00742943.3 Nucleoporin Nup43 NUP43 42.1 0 7.9 Yes No

IPI00170581.1 Isoform 2 of Nuclear pore complex protein Nup50 NUP50 46.8 0 6.27 Yes No

IPI00172580.6 Uncharacterized protein NUP54 37.3 2.16 8.22 Yes No

IPI00293533.4 Nuclear pore glycoprotein p62 NUP62 53.2 0 2.89 Yes No

IPI00909193.1

cDNA FLJ59373, highly similar to Homo sapiens pericentrin

1 (PCNT1), mRNA NUP85 52.6 0 5.12 Yes No



IPI01011651.1 177 kDa protein NUP98 177.3 0 5.92 Yes No

IPI00978175.1 Uncharacterized protein NXF1 68.1 4.9 1.89 Yes No

IPI01021468.1 Uncharacterized protein OAS2 30.9 0 1.94 Yes No

IPI00022334.1 Ornithine aminotransferase, mitochondrial OAT 48.5 7.39 11.7 Yes No

IPI00386497.3

2-oxoglutarate dehydrogenase, mitochondrial isoform 2

precursor OGDH 48.1 1.98 2.45 Yes No

IPI00170429.3

Isoform 1 of 2-oxoglutarate and iron-dependent

oxygenase domain-containing protein 1 OGFOD1 63.2 0 2.15 Yes No

IPI00937682.1 Uncharacterized protein OGFR 52.4 0 1.74 Yes No

IPI00219856.1

Isoform 2 of UDP-N-acetylglucosamine--peptide N-

acetylglucosaminyltransferase 110 kDa subunit OGT 102.9 2.6 0 Yes No

IPI00916847.1 47 kDa protein OLA1 46.9 2.54 7.03 Yes No

IPI00006721.4 Isoform 1 of Dynamin-like 120 kDa protein, mitochondrial OPA1 111.6 2.03 1.86 Yes No

IPI00983590.1 origin recognition complex subunit 3 isoform 3 ORC3 65.9 0 1.65 Yes No

IPI00917179.1 Uncharacterized protein ORC4 14.4 2.05 0 Yes No

IPI00409636.2 origin recognition complex subunit 5 isoform 2 ORC5 37.4 1.85 2.27 Yes No

IPI00024971.1 Oxysterol-binding protein 1 OSBP 89.4 0 2.32 Yes No

IPI00000581.6

cDNA FLJ56307, highly similar to Ubiquitin thioesterase

protein OTUB1 OTUB1 35.2 0 0 Yes No

IPI00924637.1 Uncharacterized protein OXSR1 51.8 0 0 Yes No

IPI00182373.2 Isoform IIa of Prolyl 4-hydroxylase subunit alpha-2 P4HA2 60.6 0 5.57 Yes No

IPI00010796.1 Protein disulfide-isomerase P4HB 57.1 20.28 32.77 Yes No

IPI01022048.1 Uncharacterized protein PA2G4 41.7 13.25 29.39 Yes No

IPI00008524.1 Isoform 1 of Polyadenylate-binding protein 1 PABPC1 70.6 31.33 48.74 Yes No

IPI00983556.1 Protein PABPC4 27.4 8.25 10.48 Yes No

IPI00376229.1 Isoform 1 of Phosphofurin acidic cluster sorting protein 1 PACS1 104.8 5.56 2.04 Yes No

IPI00221111.1

Isoform 2 of Protein kinase C and casein kinase substrate

in neurons protein 2 PACSIN2 51.3 0 5.81 Yes No

IPI00980056.1

cDNA FLJ61415, highly similar to Protein kinase C and

casein kinase substratein neurons protein 3 PACSIN3 48.2 3.18 7.01 Yes No

IPI01025379.1 Uncharacterized protein PAF1 52.8 3.39 0 Yes No

IPI00953036.1 Uncharacterized protein PAFAH1B1 23.2 1.69 2.74 Yes No

IPI00964123.1 Uncharacterized protein PAICS 45.6 17.65 26.07 Yes No

IPI00021466.5

Isoform 1 of Polyadenylate-binding protein-interacting

protein 1 PAIP1 53.5 0 8.64 Yes No

IPI00549540.3 p21-activated protein kinase-interacting protein 1 PAK1IP1 43.9 1.79 1.68 Yes No

IPI00856001.2 Isoform 5 of Palladin PALLD 127.7 4.95 13.97 Yes No

IPI00872077.3 Uncharacterized protein PAPLN 87.2 0 0 Yes No

IPI00872966.3 81 kDa protein PAPOLA 80.6 0 4.07 Yes No

IPI00011619.4

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate

synthase 1 PAPSS1 70.8 0 2.31 Yes No

IPI00909122.1 poly(A)-specific ribonuclease PARN isoform 2 PARN 66.5 3.27 8.05 Yes No

IPI00981395.1

cDNA FLJ53442, highly similar to Poly (ADP-ribose)

polymerase 1 PARP1 111.1 4.96 20.42 Yes No

IPI00296909.4 Poly [ADP-ribose] polymerase 4 PARP4 192.5 0 1.89 Yes No

IPI00879834.1 Uncharacterized protein PARVB 37.5 0 1.88 Yes No

IPI00334894.1 Isoform 3 of Protein polybromo-1 PBRM1 183.6 1.62 3.57 Yes No

IPI01022076.1 poly(rC)-binding protein 2 isoform g PCBP2 33.5 9.1 8.86 Yes No

IPI00895869.1

Isoform 2 of Propionyl-CoA carboxylase alpha chain,

mitochondrial PCCA 77 5.77 1.9 Yes No

IPI00218806.1 Isoform Short of Protocadherin alpha-C2 PCDHAC2 96.1 1.78 0 Yes No

IPI00072541.4 Isoform 3 of PCI domain-containing protein 2 PCID2 43.3 1.69 0 Yes No

IPI00797038.1

Isoform 1 of Phosphoenolpyruvate carboxykinase [GTP],

mitochondrial PCK2 70.7 0 4.28 Yes No

IPI00980953.1 Uncharacterized protein PCM1 210 5.95 8.7 Yes No

IPI00021700.3 Proliferating cell nuclear antigen PCNA;PCNA-AS1 28.8 0 0 Yes No

IPI00384280.5 Prenylcysteine oxidase 1 PCYOX1 56.6 4.19 0 Yes No

IPI00927030.1 16 kDa protein PCYT1A 15.8 0 2.84 Yes No

IPI00298409.3 Phosducin-like protein PDCL 34.3 0 2.83 Yes No

IPI01022692.1 Uncharacterized protein PDE12 52.1 0 6.16 Yes No

IPI00291205.4 cGMP-inhibited 3',5'-cyclic phosphodiesterase A PDE3A 124.9 3.75 0 Yes No

IPI00375236.2

Isoform 5 of cAMP-specific 3',5'-cyclic phosphodiesterase

4D PDE4D 84.4 1.81 1.6 Yes No

IPI00843923.1 Phosphodiesterase PDE7B2 PDE7B 57.7 0 1.65 Yes No

IPI00306301.2

pyruvate dehydrogenase E1 component subunit alpha,

somatic form, mitochondrial isoform 2 precursor PDHA1 47.5 19.14 23.08 Yes No

IPI00549885.4

Isoform 2 of Pyruvate dehydrogenase E1 component

subunit beta, mitochondrial PDHB 37.2 2.64 4.56 Yes No

IPI00975492.1 Protein PDHX 20.7 2.57 0 Yes No

IPI00009904.1 Protein disulfide-isomerase A4 PDIA4 72.9 1.81 3.24 Yes No

IPI00010414.4 PDZ and LIM domain protein 1 PDLIM1 36 2.52 0 Yes No

IPI00023122.1 Isoform 1 of PDZ and LIM domain protein 7 PDLIM7 49.8 2.11 5.2 Yes No

IPI00854642.1

Isoform 1 of Sister chromatid cohesion protein PDS5

homolog A PDS5A 150.7 10.23 6.95 Yes No

IPI00845446.3

Isoform 2 of Sister chromatid cohesion protein PDS5

homolog B PDS5B 158.2 0 4.04 Yes No

IPI00908545.1 cDNA FLJ59412 PDXDC1 76.5 0 7.77 Yes No

IPI00006702.4

cDNA FLJ56414, highly similar to Homo sapiens proline-,

glutamic acid-, leucine-rich protein 1 (PELP1), mRNA PELP1 124.8 2.24 4.77 Yes No

IPI00257882.7 Xaa-Pro dipeptidase PEPD 54.5 4.78 5.43 Yes No

IPI00878536.1 Uncharacterized protein PES1 66 1.66 4.05 Yes No

IPI00332371.9 Isoform 1 of 6-phosphofructokinase, liver type PFKL 85 10.4 14.23 Yes No

IPI00219585.4 Isoform 2 of 6-phosphofructokinase, muscle type PFKM 81.7 12.17 16.2 Yes No

IPI00009790.1 6-phosphofructokinase type C PFKP 85.5 26.83 42.45 Yes No

IPI00216691.5 Profilin-1 PFN1 15 3.19 4.43 Yes No

IPI00169383.3 Phosphoglycerate kinase 1 PGK1 44.6 28.9 41.48 Yes No

IPI00550364.8 Phosphoglucomutase-2 PGM2 68.2 3.99 1.99 Yes No

IPI00941001.1 Phosphoacetylglucosamine mutase PGM3 59.8 2.12 2.39 Yes No

IPI00970935.1 71 kDa protein PHACTR4 70.8 2.37 0 Yes No

IPI00939291.1 cDNA FLJ60207, highly similar to PHD finger protein 6 PHF6 37.6 0 2.6 Yes No

SI_II(12)


IPI00218571.1

Isoform 1 of Phosphorylase b kinase regulatory subunit

beta PHKB 124.8 0 8.42 Yes No

IPI00070943.4 Isoform 1 of Phosphatidylinositol 4-kinase alpha PI4KA 231.2 0 3.8 Yes No

IPI00980475.1

phosphatidylinositol-binding clathrin assembly protein

isoform 4 PICALM 59.9 5.01 2.1 Yes No

IPI00873758.2 Uncharacterized protein PIK3C3 94.3 0 4.03 Yes No

IPI00024006.3 Phosphoinositide 3-kinase regulatory subunit 4 PIK3R4 153 0 3.29 Yes No

IPI00922583.1

phosphatidylinositol-4-phosphate 5-kinase type-1 alpha

isoform 3 PIP5K1A 58.1 1.61 3.51 Yes No

IPI00012575.1 Pirin PIR 32.1 0 1.7 Yes No

IPI00908889.1

cDNA FLJ54065, moderately similar to Mus musculus

pitrilysin metallepetidase 1 (Pitrm1), mRNA PITRM1 106 4.37 3.29 Yes No

IPI00909829.1 Pyruvate kinase PKLR 52.6 11.25 4.95 Yes No

IPI00220644.8 Isoform M1 of Pyruvate kinase isozymes M1/M2 PKM2 58 86.07 90.98 Yes No

IPI00514557.4 Uncharacterized protein PKN2 94.1 3.38 2.39 Yes No

IPI00026952.2 Plakophilin 3b (Fragment) PKP3 88.6 0 3.99 Yes No

IPI00984072.1 Protein PKP3 12.6 1.88 1.66 Yes No

IPI00026108.2 Cytosolic phospholipase A2 PLA2G4A 85.2 6.07 15.75 Yes No

IPI00976151.1 Uncharacterized protein PLAA 66.7 4.92 6.83 Yes No

IPI00181283.2

1-phosphatidylinositol-4,5-bisphosphate

phosphodiesterase beta-3 isoform 2 PLCB3 131.1 1.76 4.28 Yes No

IPI00010604.4

Isoform 1 of 1-phosphatidylinositol-4,5-bisphosphate

phosphodiesterase epsilon-1 PLCE1 259.8 0 5.86 Yes No

IPI00016736.1

Isoform 1 of 1-phosphatidylinositol-4,5-bisphosphate

phosphodiesterase gamma-1 PLCG1 148.4 0 1.84 Yes No

IPI00328243.2 Phospholipase D3 PLD3 54.7 0 4.73 Yes No

IPI00398779.5 Isoform 4 of Plectin PLEC 515.9 414.76 517.8 Yes No

IPI00014898.3 Isoform 1 of Plectin PLEC 531.5 419 513.95 Yes No

IPI00169401.2

Isoform 2 of Pleckstrin homology domain-containing

family A member 5 PLEKHA5 134.2 6.69 1.61 Yes No

IPI00106668.5 perilipin-3 isoform 3 PLIN3 45.8 4.88 0 Yes No

IPI00939578.1

cDNA FLJ43712 fis, clone TESOP2004114, highly similar to

Homo sapiens procollagen-lysine, 2-oxoglutarate 5-

dioxygenase 2 (PLOD2), transcript variant 1, mRNA PLOD2 49.1 0 4.69 Yes No

IPI00030255.1 Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 PLOD3 84.7 5.78 6.41 Yes No

IPI00965708.1 12 kDa protein PLRG1 12 0 5.58 Yes No

IPI00853369.1 Plexin-B2 PLXNB2 205 2.58 7.09 Yes No

IPI00303999.3 Isoform PML-5 of Protein PML PML 67.4 0 5.22 Yes No

IPI00294903.3 Phosphomannomutase 1 PMM1 29.7 0 0 Yes No

IPI00166749.3 Mitochondrial-processing peptidase subunit alpha PMPCA 58.2 2.06 11.35 Yes No

IPI00927892.1 Mitochondrial-processing peptidase subunit beta PMPCB 54.3 4.22 9.06 Yes No

IPI00644388.2 Isoform 1 of PNMA-like protein 2 PNMAL2 68.8 0 0 Yes No

IPI00002649.2 Isoform 2 of Pinin PNN 67 0 0 Yes No

IPI00744711.2 Polyribonucleotide nucleotidyltransferase 1, PNPT1 85.9 3.89 6.94 Yes No

IPI00220317.3 DNA polymerase alpha catalytic subunit POLA1 165.8 3.78 1.66 Yes No

IPI00290272.2 DNA polymerase alpha subunit B POLA2 65.9 6.19 5.68 Yes No

IPI00002894.2 DNA polymerase delta catalytic subunit POLD1 123.6 1.89 6.31 Yes No

IPI00165506.4 Polymerase delta-interacting protein 2 POLDIP2 42 0 2.72 Yes No

IPI00790334.1 DNA polymerase POLE 258.2 2.08 9.38 Yes No

IPI00873948.3 DNA-directed RNA polymerase POLR2B 125.1 2.46 11.56 Yes No

IPI00930490.1 DNA-directed RNA polymerase III subunit RPC2 isoform 2 POLR3B 121 1.84 4.25 Yes No

IPI00298738.3

Similar to DNA-directed RNA polymerase, mitochondrial

precursor POLRMT 140.1 0 2.53 Yes No

IPI00293331.3 Ribonucleases P/MRP protein subunit POP1 POP1 114.6 0 5.06 Yes No

IPI00470467.5 NADPH--cytochrome P450 reductase POR 76.6 2.5 9.84 Yes No

IPI00479743.3 Isoform 1 of POTE ankyrin domain family member E POTEE 121.3 24.55 60.66 Yes No

IPI00966931.1 Protein PPA2 8.1 0 1.66 Yes No

IPI00029534.1 Amidophosphoribosyltransferase PPAT 57.4 0 5.76 Yes No

IPI00419585.9 Peptidyl-prolyl cis-trans isomerase A PPIA 18 0 4.76 Yes No

IPI00646304.4 Peptidyl-prolyl cis-trans isomerase B PPIB 23.7 0 3.03 Yes No

IPI00003927.5 Peptidyl-prolyl cis-trans isomerase D PPID 40.7 0 2.03 Yes No

IPI01015454.1 Uncharacterized protein PPM1G 57.3 3.51 6.41 Yes No

IPI00007694.5 Isoform 1 of Protein phosphatase methylesterase 1 PPME1 42.3 1.97 2.67 Yes No

IPI00902512.3 Serine/threonine-protein phosphatase PPP1CA 46 4.25 2.37 Yes No

IPI00980919.1 Serine/threonine-protein phosphatase PPP1CA 28.9 4.25 2.34 Yes No

IPI01022374.1 Protein PPP1R12A 40.6 0 1.63 Yes No

IPI00983167.1 Protein PPP2CB 6.1 0 2.39 Yes No

IPI00908543.1

cDNA FLJ56133, highly similar to Serine/threonine-protein

phosphatase 2A 65 kDa regulatory subunit A alpha isoform PPP2R1A 61.5 2.65 10.66 Yes No

IPI00893197.1 Protein phosphatase 2A, regulatory subunit B' isoform b PPP2R4 41.7 5.11 6.83 Yes No

IPI00219544.1

Isoform Delta-3 of Serine/threonine-protein phosphatase

2A 56 kDa regulatory subunit delta isoform PPP2R5D 58.4 3.79 3.06 Yes No

IPI00947527.2 Uncharacterized protein PPP4R2 7.3 0 0 Yes No

IPI01012187.1

cDNA, FLJ79426, highly similar to Serine/threonine-protein

phosphatase 6 PPP6C 18 0 2.46 Yes No

IPI00719345.1

Isoform 3 of Serine/threonine-protein phosphatase 6

regulatory subunit 3 PPP6R3 88.9 1.82 7.94 Yes No

IPI00008164.2 Prolyl endopeptidase PREP 80.6 6.69 4.51 Yes No

IPI00552692.3

Isoform 3 of Phosphatidylinositol 3,4,5-trisphosphate-

dependent Rac exchanger 1 protein PREX1 175.7 0 0 Yes No

IPI00027705.2 Isoform 1 of DNA primase large subunit PRIM2 58.8 7.47 8.4 Yes No

IPI00792482.1

Isoform 1 of 5'-AMP-activated protein kinase catalytic

subunit alpha-1 PRKAA1 64 2.58 4.12 Yes No

IPI00217960.1

Isoform 2 of cAMP-dependent protein kinase catalytic

subunit alpha PRKACA 39.8 0 1.96 Yes No

IPI00021831.1

cAMP-dependent protein kinase type I-alpha regulatory

subunit PRKAR1A 43 2.32 2.41 Yes No

IPI00925327.2 Protein PRKAR2A 23 0 0 Yes No

IPI00816171.1 serine/threonine-protein kinase D2 isoform B PRKD2 80.1 0 2.74 Yes No

IPI00296337.2

Isoform 1 of DNA-dependent protein kinase catalytic

subunit PRKDC 468.8 73.08 128.68 Yes No

IPI00922411.1 protein arginine N-methyltransferase 3 isoform 3 PRMT3 51.5 0 0 Yes No

IPI00441473.3 Protein arginine N-methyltransferase 5 PRMT5 72.6 2.84 6.87 Yes No

IPI00004968.1 Pre-mRNA-processing factor 19 PRPF19 55.1 8.87 4.77 Yes No

IPI00738596.2

cDNA FLJ59251, highly similar to U4/U6 small nuclear

ribonucleoprotein Prp31 PRPF31 50.1 0 4.72 Yes No

IPI00150269.1 Isoform 1 of U4/U6 small nuclear ribonucleoprotein Prp4 PRPF4 58.4 1.83 3.22 Yes No

IPI01018784.1

cDNA FLJ59286, highly similar to Pre-mRNA-processing

factor 40 homolog A (Fragment) PRPF40A 92.8 2.04 5.28 Yes No

IPI01012099.1 102 kDa protein PRPF6 102.4 1.73 15.06 Yes No

IPI00219616.7 Ribose-phosphate pyrophosphokinase 1 PRPS1 34.8 2.42 5.71 Yes No

IPI00291578.7

Isoform 1 of Phosphoribosyl pyrophosphate synthase-

associated protein 1 PRPSAP1 39.4 0 8.56 Yes No

IPI00784461.2 Isoform 1 of Proline-rich protein 5-like PRR5L 40.8 0 0 Yes No

IPI00015614.4 Isoform A of Trypsin-3 PRSS3 32.5 2.95 0 Yes No

IPI00001734.3 Phosphoserine aminotransferase PSAT1 45.3 5.18 27.28 Yes No

IPI01015427.1

cDNA FLJ58247, highly similar to 26S protease regulatory

subunit 4 PSMC1 41.1 8.24 12.63 Yes No

IPI00021435.3 26S protease regulatory subunit 7 PSMC2 48.6 4.02 13.33 Yes No

IPI00979951.1 Uncharacterized protein PSMC3 26.6 0 2.04 Yes No

IPI00216770.1 Isoform 2 of 26S protease regulatory subunit 6B PSMC4 43.5 0 0 Yes No

IPI00745502.2 26S protease regulatory subunit 8 isoform 2 PSMC5 44.8 10.22 12.4 Yes No

IPI00926977.1 26S protease regulatory subunit 10B PSMC6 45.8 2.56 4.82 Yes No

IPI00456695.1

Isoform 2 of 26S proteasome non-ATPase regulatory

subunit 1 PSMD1 102.2 2.37 8.88 Yes No

IPI00335069.2

26S proteasome non-ATPase regulatory subunit 12

isoform 2 PSMD12 50.5 2.19 6.63 Yes No

SI_II(13)


IPI00375380.4

Isoform 2 of 26S proteasome non-ATPase regulatory

subunit 13 PSMD13 42.9 4.66 6.71 Yes No

IPI00024821.1 26S proteasome non-ATPase regulatory subunit 14 PSMD14 34.6 3.48 2.93 Yes No

IPI00012268.3 26S proteasome non-ATPase regulatory subunit 2 PSMD2 100.1 10.44 11.86 Yes No

IPI00853609.2 Uncharacterized protein PSMD4 21.8 0 2.93 Yes No

IPI00909766.1

cDNA FLJ52877, highly similar to 26S proteasome non-

ATPase regulatory subunit 5 PSMD5 51.3 11.76 20.53 Yes No

IPI00014151.3 26S proteasome non-ATPase regulatory subunit 6 PSMD6 45.5 0 2.68 Yes No

IPI00005260.4 Isoform 1 of Proteasome activator complex subunit 4 PSME4 211.2 0 7.56 Yes No

IPI01009339.1 27 kDa protein PSPC1 27.3 0 3.97 Yes No

IPI00179964.5 Isoform 1 of Polypyrimidine tract-binding protein 1 PTBP1 57.2 10.52 11.3 Yes No

IPI00783302.1

Isoform 1 of Pentatricopeptide repeat-containing protein

3, mitochondrial PTCD3 78.5 3.77 6.67 Yes No

IPI00168813.1 Isoform 3 of Inactive tyrosine-protein kinase 7 PTK7 103.5 3.03 6.76 Yes No

IPI00787433.2

cDNA FLJ54138, highly similar to Homo sapiens butyrate-

induced transcript 1 (HSPC121), mRNA PTPLAD1 36.4 3.83 3.5 Yes No

IPI01014223.1

cDNA FLJ50851, highly similar to Tyrosine-protein

phosphatase non-receptor type1 PTPN1 41.2 5.15 6.03 Yes No

IPI00855912.1 Isoform 6 of Poly(U)-binding-splicing factor PUF60 PUF60 55.4 4.09 5.67 Yes No

IPI00982610.1 Protein PUM1 95.7 1.66 2.44 Yes No

IPI00945342.1 Uncharacterized protein PUS7 50.5 3.28 0 Yes No

IPI00016112.6 Isoform 1 of Peroxidasin homolog PXDN 165.2 0 3.85 Yes No

IPI00985173.1 Protein PXK 34.8 2.08 0 Yes No

IPI00004358.4 Glycogen phosphorylase, brain form PYGB 96.6 4.81 5.04 Yes No

IPI00943894.1 glycogen phosphorylase, liver form isoform 2 PYGL 93.1 10.29 20.61 Yes No

IPI00925511.1

cDNA FLJ54314, highly similar to Glutaminyl-tRNA

synthetase QARS 86.5 6.36 23.97 Yes No

IPI00554590.1

Isoform 1 of Rab3 GTPase-activating protein non-catalytic

subunit RAB3GAP2 155.9 2 4.95 Yes No

IPI00016702.4 Isoform 1 of Rab GTPase-activating protein 1 RABGAP1 121.7 5.85 1.6 Yes No

IPI00924641.1 Isoform 2 of Rab5 GDP/GTP exchange factor RABGEF1;KCTD7 56.9 2.37 1.8 Yes No

IPI00022664.3 Geranylgeranyl transferase type-2 subunit alpha RABGGTA 65 1.98 6.08 Yes No

IPI00006715.3 Double-strand-break repair protein rad21 homolog RAD21 71.6 3.04 4.36 Yes No

IPI00008219.1 UV excision repair protein RAD23 homolog A RAD23A 39.6 6.14 2.04 Yes No

IPI00549205.1 Isoform 2 of DNA repair protein RAD50 RAD50 154.5 19.32 14.79 Yes No

IPI00019733.1 mRNA export factor RAE1 40.9 1.66 1.9 Yes No

IPI00967485.1 ankycorbin isoform b RAI14 106.8 2.11 12.02 Yes No

IPI00642213.1 Uncharacterized protein RALY 24.7 2.67 6.07 Yes No

IPI00221325.3 E3 SUMO-protein ligase RanBP2 RANBP2 358 6.15 32.93 Yes No

IPI00179121.4 Isoform 4 of Ran-binding protein 3 RANBP3 42.3 0 0 Yes No

IPI00554545.2

cDNA, FLJ93856, Homo sapiens RAN binding protein 9

(RANBP9), mRNA RANBP9 55 2.02 2.65 Yes No

IPI00294879.1 Ran GTPase-activating protein 1 RANGAP1 63.5 8.94 15.8 Yes No

IPI00795109.1

cDNA, FLJ79092, highly similar to GTP-binding nuclear

protein Ran RANP1;RAN 14.7 2.72 0 Yes No

IPI00965386.1 Protein RAP1GDS1 27.5 0 2.54 Yes No

IPI00743280.2

Isoform RMO1c of Ras-associated and pleckstrin homology

domains-containing protein 1 RAPH1 66.9 0 5.44 Yes No

IPI00217670.1 Isoform 1 of Ribonucleoprotein PTB-binding 1 RAVER1 63.8 0 3.46 Yes No

IPI00395865.4 Histone-binding protein RBBP7 RBBP7 47.8 7.46 19.81 Yes No

IPI00550308.1 RNA-binding protein 12 RBM12 97.3 2.84 8.44 Yes No

IPI00013174.2 Isoform 1 of RNA-binding protein 14

RBM14;RBM14-

RBM4 69.4 8.07 8.03 Yes No

IPI00176706.1 Splicing factor 45 RBM17 44.9 0 1.64 Yes No

IPI00292975.4 RNA-binding protein 27 RBM27 118.6 0 2.36 Yes No

IPI00304187.8 RNA-binding protein 28 RBM28 85.7 0 6.96 Yes No

IPI00647282.1 Uncharacterized protein RBM39 27.1 0 2.93 Yes No

IPI00003704.4 Isoform 1 of RNA-binding protein 4

RBM4;RBM14;RBM

14-RBM4 40.3 3.89 1.74 Yes No

IPI00747309.1 Isoform 1 of Regulator of chromosome condensation RCC1 44.9 4.19 4.06 Yes No

IPI00465044.2 Protein RCC2 RCC2 56 3.62 9.74 Yes No

IPI00178431.12 ATP-dependent DNA helicase Q1 RECQL 73.4 9.01 11.36 Yes No

IPI00915989.1 Protein RETSAT 43.8 4.92 3.05 Yes No

IPI00373882.2 replication factor C subunit 3 isoform 2 RFC3 34.7 2.16 5.24 Yes No

IPI00017381.1 Replication factor C subunit 4 RFC4 39.7 4.25 5.28 Yes No

IPI00925306.1 replication factor C subunit 5 isoform 2 RFC5 36.1 4.41 4 Yes No

IPI00099996.2 Mitochondrial ribonuclease P protein 1 RG9MTD1 47.3 0 1.98 Yes No

IPI00465059.4 Isoform 1 of Mitochondrial Rho GTPase 2 RHOT2 68.1 3.48 0 Yes No

IPI00749304.2 Isoform 2 of Synembryn-A RIC8A 58.9 0 1.7 Yes No

IPI00973063.1 Uncharacterized protein RNF14 6.9 0 2.04 Yes No

IPI00642126.6 Isoform 1 of Protein ALO17 RNF213 174.8 2.42 0 Yes No

IPI00290648.3 Isoform 4 of E3 ubiquitin-protein ligase BRE1B RNF40 102 11.4 2.78 Yes No

IPI01015903.1 cDNA FLJ50195, highly similar to mRNA capping enzyme RNGTT 59 0 0 Yes No

IPI00550069.3 Ribonuclease inhibitor RNH1 49.9 6.45 9.15 Yes No

IPI00647400.1 Uncharacterized protein RNPEP 68.1 5.25 0 Yes No

IPI00307155.8 Rho-associated protein kinase 2 ROCK2 160.8 12.55 17.86 Yes No

IPI00480153.1 Uncharacterized protein ROR2 79.2 0 2.63 Yes No

IPI00020127.1 Replication protein A 70 kDa DNA-binding subunit RPA1 68.1 3.22 10.3 Yes No

IPI00448515.1 Isoform 2 of RNA polymerase II-associated protein 3 RPAP3 71.8 6.17 1.66 Yes No

IPI00647168.1 Ribosomal protein L11 RPL11 14.9 3.01 0 Yes No

IPI00926202.1 Ribosomal protein L15 RPL15 20.5 0 1.63 Yes No

IPI01021193.1 Uncharacterized protein RPL18 18.1 0 6.39 Yes No

IPI00946221.1 Uncharacterized protein RPL24 17.5 2.32 3.86 Yes No

IPI00980350.1 Uncharacterized protein RPL27A 10.1 2.58 2.37 Yes No

IPI00003918.6 60S ribosomal protein L4 RPL4 47.7 7.58 11.69 Yes No

IPI01025005.1 Uncharacterized protein RPL6 25.9 2.2 1.88 Yes No

IPI00299573.12 60S ribosomal protein L7a RPL7A;SNORD24 30 4.28 9.1 Yes No

IPI00794746.1 Uncharacterized protein RPL7P32;RPL7 24.4 0 2.11 Yes No

IPI01022462.1 Uncharacterized protein RPLP0 26.9 13.53 13.71 Yes No

IPI00793589.2

cDNA FLJ51740, highly similar to Dolichyl-

diphosphooligosaccharide--proteinglycosyltransferase 67

kDa subunit RPN1 65.8 13.5 23.54 Yes No

IPI00026271.5 40S ribosomal protein S14 RPS14 16.3 0 3.69 Yes No

IPI00221092.8 40S ribosomal protein S16 RPS16 16.4 1.9 0 Yes No

IPI00013296.3 40S ribosomal protein S18 RPS18;RPS18P9 17.7 0 4.16 Yes No

IPI00012750.3 40S ribosomal protein S25 RPS25 13.7 2.02 0 Yes No

IPI00974484.1 28 kDa protein RPS3 28.5 4.14 6.07 Yes No

IPI00640510.2 Uncharacterized protein RPS6KA1 81.1 4.87 2.31 Yes No


IPI00979136.1 Ribonucleoside-diphosphate reductase RRM1 86.4 9.34 26.55 Yes No

IPI00011118.2

ribonucleoside-diphosphate reductase subunit M2 isoform

1 RRM2 51.1 6.57 10.02 Yes No

IPI00642046.1 RSL1D1 protein RSL1D1 54.8 0 6.7 Yes No

IPI00788942.1 Isoform 2 of RuvB-like 1 RUVBL1 42.1 0 15.93 Yes No

IPI00009104.7 RuvB-like 2 RUVBL2 51.1 14.44 27.55 Yes No

IPI00924492.1 Uncharacterized protein SACM1L 55.5 0 5.45 Yes No

IPI01011453.1 SUMO-activating enzyme subunit 1 isoform c SAE1 29.8 4.69 2.61 Yes No

IPI01018318.1

cDNA FLJ54744, highly similar to Scaffold attachment

factor B SAFB 86 2.34 4.07 Yes No

IPI00943982.1

Isoform 2 of SAM domain and HD domain-containing

protein 1 SAMHD1 69.4 1.95 6.15 Yes No

IPI00412713.4 Sorting and assembly machinery component 50 homolog SAMM50 51.9 5.71 4.14 Yes No

IPI00220637.5 Seryl-tRNA synthetase, cytoplasmic SARS 58.7 3.32 10.46 Yes No

IPI00719256.2 Uncharacterized protein SART3 105.5 3.01 5.31 Yes No

IPI00879441.1 Isoform 3 of Protein strawberry notch homolog 1 SBNO1 154 5.75 7.04 Yes No

IPI00329600.3 Probable saccharopine dehydrogenase SCCPDH 47.1 1.87 4.58 Yes No

IPI01015213.1

cDNA FLJ58612, highly similar to Sec1 family domain-

containing protein 1 SCFD1 51.5 0 3.51 Yes No

SI_II(14)


IPI00410666.2 Isoform 3 of Protein scribble homolog SCRIB 177.6 3.56 5.35 Yes No

IPI00978883.1 Uncharacterized protein SCYL1 73.6 0 0 Yes No

IPI00965327.1 Uncharacterized protein SDHA 63.5 0 23.68 Yes No

IPI01021269.1 6 kDa protein SDSL 6.2 0 2.75 Yes No

IPI00641384.2 protein transport protein Sec16A SEC16A 251.7 2.58 2.65 Yes No

IPI01011937.1 Uncharacterized protein SEC23A 82.9 0 2.53 Yes No

IPI01011794.2 Uncharacterized protein SEC23IP 89.6 4.63 2.52 Yes No

IPI00873472.1 Isoform 1 of Protein transport protein Sec24A SEC24A 119.7 0 1.97 Yes No

IPI00024661.5 Protein transport protein Sec24C SEC24C 118.2 0 4.02 Yes No

IPI00966967.1 Uncharacterized protein SEC24D 74.9 3.53 3.63 Yes No

IPI00853220.1 Isoform 6 of Protein transport protein Sec31A SEC31A 117.6 7.95 1.95 Yes No

IPI00903261.1

cDNA FLJ43868 fis, clone TESTI4008050, highly similar to

Translocation protein SEC63 homolog SEC63 43.4 0 6.94 Yes No

IPI00640517.2 Uncharacterized protein SEPHS1 18.5 2.65 0 Yes No

IPI00927054.1 Uncharacterized protein SEPT10 52.1 0 2.72 Yes No

IPI00216139.3 Isoform I of Septin-6 SEPT6 48.8 7.3 13.88 Yes No

IPI01009775.1 cDNA FLJ52574, highly similar to Septin-7 SEPT7 44.6 9.85 10.29 Yes No

IPI00883870.1 septin-9 isoform d SEPT9 52.9 4.15 5.03 Yes No

IPI00783625.2 Isoform 1 of Serpin B5 SERPINB5 42.1 4.23 23.48 Yes No

IPI00413451.3 Serpin B6 SERPINB6 46.3 4.03 13.57 Yes No

IPI00032140.4 Serpin H1 SERPINH1 46.4 11.65 14.3 Yes No

IPI00844040.2 cDNA FLJ59759, highly similar to Protein SET SET 31.1 0 4.34 Yes No

IPI00979086.1 Uncharacterized protein SETDB1 139.3 5.63 0 Yes No

IPI01010147.2 Uncharacterized protein SF3A1 77.4 1.75 9.45 Yes No

IPI00908449.1

cDNA FLJ58737, highly similar to Splicing factor 3A subunit

3 SF3A3 52.4 0 5.82 Yes No

IPI00026089.4 Splicing factor 3B subunit 1 SF3B1 145.7 12.35 12.45 Yes No

IPI00477803.4

Putative uncharacterized protein DKFZp781L0540

(Fragment) SF3B2 90 0 4.81 Yes No

IPI00099463.2 Sphingosine-1-phosphate lyase 1 SGPL1 63.5 2.2 0 Yes No

IPI00100731.3 SH2 domain-containing protein 4A SH2D4A 52.7 0 0 Yes No

IPI00005030.5 Isoform 1 of SH3 domain-binding protein 4 SH3BP4 107.4 3.81 3.34 Yes No

IPI00644418.3 endophilin-A2 isoform 3 SH3GL1 34.5 3.64 3.24 Yes No

IPI01015924.1 Serine hydroxymethyltransferase SHMT1 37.6 0 2.95 Yes No

IPI00479890.4 Isoform 2 of Leucine-rich repeat protein SHOC-2 SHOC2 59.7 0 1.78 Yes No

IPI00759728.1 Isoform 2 of Sialate O-acetylesterase SIAE 54.5 1.84 0 Yes No

IPI00981200.1 Uncharacterized protein SIDT2 12.4 0 1.89 Yes No

IPI00170596.1 Paired amphipathic helix protein Sin3a SIN3A 145.1 4.33 5.74 Yes No

IPI01026217.1

cDNA FLJ58647, highly similar to Signal-induced

proliferation-associated 1-likeprotein 1 SIPA1L1 197.3 8.02 4.01 Yes No

IPI01011073.2 Uncharacterized protein SKIV2L2 106.7 1.67 10.81 Yes No

IPI00006666.1 Monocarboxylate transporter 4 SLC16A3 49.4 0 4.03 Yes No

IPI00006620.2 Monocarboxylate transporter 7 SLC16A6 57.4 0 1.8 Yes No

IPI00922487.1 neutral amino acid transporter B(0) isoform 2 SLC1A5 33.7 0 4.48 Yes No

IPI00337494.7

Isoform 1 of Calcium-binding mitochondrial carrier protein

SCaMC-1 SLC25A24 53.3 4.7 9.47 Yes No

IPI00412147.1 Long-chain fatty acid transport protein 4 SLC27A4 72 0 4.25 Yes No

IPI00002483.5 Zinc transporter 1 SLC30A1 55.3 0 4.06 Yes No

IPI00302605.3 Zinc transporter 7 SLC30A7 41.6 0 1.93 Yes No

IPI01011348.1

cDNA FLJ59462, highly similar to Adenosine 3'-phospho 5'-

phosphosulfate transporter 1 SLC35B2 32.6 0 1.93 Yes No

IPI00023030.4 Sodium-coupled neutral amino acid transporter 1 SLC38A1 54 1.85 3.63 Yes No

IPI00641465.1

Isoform 2 of Sodium-coupled neutral amino acid

transporter 5 SLC38A5 45.9 0 2.2 Yes No

IPI00975884.1 Uncharacterized protein SLC39A14 9.6 3.39 3.63 Yes No

IPI00027493.1 Isoform 2 of 4F2 cell-surface antigen heavy chain SLC3A2 57.9 40.15 55.11 Yes No

IPI00639817.2 sodium bicarbonate transporter-like protein 11 isoform 3 SLC4A11 98.1 1.8 0 Yes No

IPI00306749.8 Kanadaptin SLC4A1AP 88.8 0 6.82 Yes No

IPI00900328.3 transcription activator BRG1 isoform F SMARCA4 181.1 2.36 11.48 Yes No

IPI01013681.1 Uncharacterized protein SMARCA5 116.7 10.28 6.09 Yes No

IPI00150057.6 Isoform 2 of SWI/SNF complex subunit SMARCC2 SMARCC2 124.8 0 4.69 Yes No

IPI00939917.1

Isoform 3 of SWI/SNF-related matrix-associated actin-

dependent regulator of chromatin subfamily D member 2 SMARCD2 55.2 0 0 Yes No

IPI00007927.4

Isoform 1 of Structural maintenance of chromosomes

protein 2 SMC2 135.6 24.11 35.26 Yes No

IPI00219420.3 Structural maintenance of chromosomes protein 3 SMC3 141.5 14.82 13 Yes No

IPI00793575.2 Uncharacterized protein SMC4 144.4 17.95 18.6 Yes No

IPI00890837.1

Isoform 1 of Structural maintenance of chromosomes

flexible hinge domain-containing protein 1 SMCHD1 226.2 3.53 7.64 Yes No

IPI00384714.2

Isoform 4 of Serine/threonine-protein phosphatase 4

regulatory subunit 3A SMEK1 67.8 0 3.49 Yes No

IPI00939320.1 Isoform 1 of Serine/threonine-protein kinase SMG1 SMG1 410.2 6.39 4.61 Yes No

IPI00005102.3 Isoform 1 of Spermine synthase SMS 41.2 2.38 4.72 Yes No

IPI00305833.3 WD40 repeat-containing protein SMU1 SMU1 57.5 6.62 16.03 Yes No

IPI00915274.1 H/ACA ribonucleoprotein complex subunit 4 isoform 2 SNORA56;DKC1 57 0 1.86 Yes No

IPI00853485.1 Uncharacterized protein SNORA70;RPL10 20.2 1.96 0 Yes No

IPI00411937.4 Nucleolar protein 56

SNORD110;SNORD8

6;NOP56 66 6.01 3.52 Yes No

IPI00003886.3 Isoform 2 of Guanine nucleotide-binding protein-like 3 SNORD19B;GNL3 60.5 7.98 10.64 Yes No

IPI00640037.2

cDNA FLJ38496 fis, clone FELIV1000137, highly similar to

60S RIBOSOMAL PROTEIN L5 SNORD21;RPL5 28 2.06 9.59 Yes No

IPI00651660.1 60S ribosomal protein L3 isoform b SNORD43;RPL3 40.1 0 5.64 Yes No

IPI00420014.2

Isoform 1 of U5 small nuclear ribonucleoprotein 200 kDa

helicase SNRNP200 244.4 26.82 40.57 Yes No

IPI00556082.3 Sorting nexin 1 isoform a variant (Fragment) SNX1 50.1 0 2.9 Yes No

IPI00964716.1 cDNA FLJ51799, highly similar to Sorting nexin-2 SNX2 46.1 2.9 0 Yes No

IPI00328097.4 Isoform 3 of Sorting nexin-27 SNX27 52.3 0 2.43 Yes No

IPI00396025.8 Isoform 1 of Sorting nexin-32 SNX32 46.4 3.96 0 Yes No

IPI00001883.1 Sorting nexin-9 SNX9 66.5 6.49 7.72 Yes No

IPI00218621.2 Isoform E of Protein SON SON 228 2.26 5.72 Yes No

IPI00220989.1 Isoform 2 of Transcription factor SOX-30 SOX30 54.3 2.7 2.36 Yes No

IPI00011675.1 Isoform Sp100-HMG of Nuclear autoantigen Sp-100 SP100 100.4 1.83 2.54 Yes No

IPI00514111.4 Isoform 1 of Spermatogenesis-associated protein 1 SPATA1 50.3 1.67 1.85 Yes No

IPI00329583.8 Isoform 1 of Spermatogenesis-associated protein 5 SPATA5 97.8 5.82 7.84 Yes No

IPI00031608.4

Isoform 1 of Spermatogenesis-associated protein 5-like

protein 1 SPATA5L1 80.7 2.37 4.14 Yes No

IPI00329345.7 Spermatogenesis-associated serine-rich protein 2 SPATS2 59.5 0 1.88 Yes No

IPI00012645.2 Isoform 1 of Spectrin beta chain, brain 2 SPTBN2 271.2 7.06 14.48 Yes No

IPI00219168.7 Spectrin beta chain, brain 4 SPTBN5 416.6 1.75 5.92 Yes No

IPI00292020.3 Spermidine synthase SRM 33.8 0 7.11 Yes No

IPI01025877.1 49 kDa protein SRP54 48.6 2.77 5.68 Yes No

IPI00102936.3 Isoform 2 of Signal recognition particle 68 kDa protein SRP68 67.3 3.93 3.65 Yes No

IPI00966751.1 CaM kinase II isoform SRP72 67.8 0 2.11 Yes No

IPI00966190.2 Uncharacterized protein SRPK1 62 0 3.19 Yes No

IPI00220038.1 Isoform 2 of Serrate RNA effector molecule homolog SRRT 100.2 1.72 9.75 Yes No

IPI00218592.5 Isoform ASF-3 of Serine/arginine-rich splicing factor 1 SRSF1 22.4 0 2.26 Yes No

IPI00916802.1 Uncharacterized protein SSB 41.8 0 4.68 Yes No

IPI00830086.1 Isoform 2 of Sperm-specific antigen 2 SSFA2 121.4 3.53 4.99 Yes No

IPI00005154.1 FACT complex subunit SSRP1 SSRP1 81 1.64 5.37 Yes No

IPI01013030.1 Uncharacterized protein STAG2 134 4.51 4.47 Yes No

IPI00218188.1

Isoform Beta of Signal transducer and activator of

transcription 1-alpha/beta STAT1 83 8.01 16.63 Yes No

IPI01013059.1 76 kDa protein STAT3 76.1 2.42 4.68 Yes No

IPI01010362.1

cDNA FLJ54464, highly similar to Signal transducer and

activator of transcription 5A STAT5A 86.9 5.31 0 Yes No

IPI00479946.4 STIP1 protein STIP1 68 17.79 27.32 Yes No

IPI00552426.2 Uncharacterized protein STK24 24.7 1.82 4.5 Yes No

SI_II(15)


IPI00294536.2

cDNA FLJ51909, highly similar to Serine-threonine kinase

receptor-associatedprotein STRAP 39.8 7.16 9.69 Yes No

IPI00413860.4 Isoform 2 of Spermatid perinuclear RNA-binding protein STRBP 71.9 1.86 1.99 Yes No

IPI00152377.1


glycosyltransferase subunit STT3B STT3B 93.6 2.99 6.77 Yes No

IPI00922881.1

cDNA FLJ55070, highly similar to Syntaxin-binding protein

1 STXBP1 49.8 4.01 0 Yes No

IPI00297626.4 Syntaxin-binding protein 3 STXBP3 67.7 0 2.58 Yes No

IPI00640500.1 Isoform 3 of Syntaxin-binding protein 5 STXBP5 121.5 2.61 7.05 Yes No

IPI00945507.1

succinyl-CoA ligase [GDP-forming] subunit beta,

mitochondrial isoform 1 precursor SUCLG2 47.7 5.39 11.39 Yes No

IPI00916532.1 Uncharacterized protein SUMO1 7.2 0 1.75 Yes No

IPI00026970.4 FACT complex subunit SPT16 SUPT16H 119.8 5.79 10.61 Yes No

IPI01010195.1

cDNA FLJ55629, highly similar to Transcription elongation

factor SPT5 SUPT5H 118.1 1.85 2.48 Yes No

IPI00784161.1 Isoform 1 of Transcription elongation factor SPT6 SUPT6H 198.9 6.68 4.44 Yes No

IPI00412650.2 Isoform 1 of Supervillin SVIL 247.6 3.47 1.71 Yes No

IPI00307200.9 Switch-associated protein 70 SWAP70 69 1.64 3.31 Yes No

IPI00239405.4 Isoform 1 of Nesprin-2 SYNE2 795.9 8.3 6.03 Yes No

IPI00395625.1

TGF-beta-activated kinase 1 and MAP3K7-binding protein

1 isoform beta TAB1 49.9 0 3.12 Yes No

IPI00001382.2 Isoform 2 of Antigen peptide transporter 2 TAP2 72 0 2.3 Yes No

IPI00910355.1

cDNA FLJ58188, highly similar to TAR DNA-binding protein

43 TARDBP 31.8 4.57 5.57 Yes No

IPI00329633.5 Threonyl-tRNA synthetase, cytoplasmic TARS 83.4 22.3 47.43 Yes No

IPI00910189.1

cDNA FLJ52892, highly similar to Homo sapiens threonyl-

tRNA synthetase-like 1 (TARSL1), mRNA TARS2 18.5 1.73 0 Yes No

IPI00794613.5 Isoform 2 of TBC1 domain family member 15 TBC1D15 77.3 0 5.1 Yes No

IPI00097495.5 Isoform 1 of TBC1 domain family member 2A TBC1D2 105.3 5.52 11.97 Yes No

IPI00879800.1 Isoform 2 of TBC1 domain family member 2B TBC1D2B 103.9 0 2 Yes No

IPI00942269.2 TBC1 domain family member 4 TBC1D4 146.5 2.34 5.01 Yes No

IPI00643529.1 Uncharacterized protein TBC1D7 15.3 0 4.23 Yes No

IPI00640908.4 Isoform 2 of TBC1 domain family member 9B TBC1D9B 138.6 2.11 7.6 Yes No

IPI00396203.7 Isoform 1 of Tubulin-specific chaperone D TBCD 132.5 0 14.83 Yes No

IPI00293613.2 Serine/threonine-protein kinase TBK1 TBK1 83.6 0 3.56 Yes No

IPI01012500.1 Uncharacterized protein TBL1Y 39.7 1.79 2.11 Yes No

IPI00927538.1 Uncharacterized protein TBL2 45.9 4.92 1.63 Yes No

IPI00477971.4 Transducin beta-like protein 3 TBL3 89 0 7.32 Yes No

IPI00333215.1 Isoform 1 of Transcription elongation factor A protein 1 TCEA1 33.9 4.83 3.1 Yes No

IPI01022399.1 116 kDa protein TCERG1 116.1 1.73 2.17 Yes No

IPI00980862.1 Uncharacterized protein TCIRG1 19.4 0 2.91 Yes No

IPI00644037.1

cDNA FLJ36768 fis, clone ADIPS1000064, highly similar to

Synaptic glycoprotein SC2 TECR 37.4 2.38 2.19 Yes No

IPI00016868.3 Telomere length regulation protein TEL2 homolog TELO2 91.7 3.62 6.4 Yes No

IPI00878833.2 Uncharacterized protein TES 9 0 2.55 Yes No

IPI00297452.5 Tyrosine-protein kinase receptor TFG 88.6 5.7 0 Yes No

IPI00556324.1

Transforming growth factor, beta-induced, 68kDa variant

(Fragment) TGFBI 25.8 0 6.12 Yes No

IPI00759539.1 Isoform NELF-D of Negative elongation factor C/D TH1L 65.4 0 2.46 Yes No

IPI00296099.6 Thrombospondin-1 THBS1 129.3 0 2.44 Yes No

IPI00016287.5 Threonine synthase-like 1 THNSL1 83 0 2.61 Yes No

IPI00549189.4 Thimet oligopeptidase THOP1 78.8 5.1 5.59 Yes No

IPI01013673.1 Uncharacterized protein TIAL1 27.9 0 4.66 Yes No

IPI00554678.2 Isoform 2 of Protein timeless homolog TIMELESS 138.4 2.63 1.64 Yes No

IPI00335824.5 Isoform Short of Tight junction protein ZO-1 TJP1 186.9 5.03 8.5 Yes No

IPI00942979.1 Transketolase TKT 67.8 59.17 65.99 Yes No

IPI00478062.2 32 kDa protein TLE2 32 0 2.07 Yes No

IPI00219299.4 Talin-2 TLN2 271.4 0 21.49 Yes No

IPI00644458.1 Uncharacterized protein TM9SF3 29.9 0 0 Yes No

IPI01026466.1 17 kDa protein TMED10 16.9 0 1.64 Yes No

IPI01011928.1

cDNA FLJ60544, highly similar to Transmembrane protein

TPARL TMEM165 28.4 0 2.48 Yes No

IPI00447589.2 Isoform 3 of Transmembrane protein 209 TMEM209 58.2 0 0 Yes No

IPI00843881.1 Isoform 2 of Transmembrane protein 214 TMEM214 72 4.8 2.03 Yes No

IPI00967533.1 Uncharacterized protein TMEM33 25.2 0 5.06 Yes No

IPI00074330.7 Isoform 3 of Nucleoporin NDC1 TMEM48 63.5 2.2 4.51 Yes No

IPI00005087.1 Tropomodulin-3 TMOD3 39.6 2.48 0 Yes No

IPI00216230.3 Lamina-associated polypeptide 2, isoform alpha TMPO 75.4 5.32 11.11 Yes No

IPI00410473.4 Transmembrane protease serine 11F TMPRSS11F 49.4 0 0 Yes No

IPI00908319.1

cDNA FLJ51570, highly similar to Thioredoxin domain-

containing protein 1 TMX1 22.4 2.16 2.11 Yes No

IPI00555585.3 Uncharacterized protein TMX3 47.9 0 3.52 Yes No

IPI00304866.4 Tumor necrosis factor alpha-induced protein 2 TNFAIP2 72.6 0 2.47 Yes No

IPI00455127.2 Isoform 3 of Transportin-3 TNPO3 102.5 20.03 26.75 Yes No

IPI00009946.4 Mitochondrial import receptor subunit TOM34 TOMM34 34.5 0 2.16 Yes No

IPI00376117.1

Isoform 2 of Mitochondrial import receptor subunit

TOM40 homolog TOMM40 34.4 2.11 2.39 Yes No

IPI00015602.1 Mitochondrial import receptor subunit TOM70 TOMM70A 67.4 6.71 7.62 Yes No

IPI00984931.1 Uncharacterized protein TOP1MT 58.6 1.64 1.69 Yes No

IPI01011132.1 Isoform 1 of DNA topoisomerase 2-alpha TOP2A 174.3 3.39 9.79 Yes No

IPI00217709.1 Isoform Beta-1 of DNA topoisomerase 2-beta TOP2B 182.5 8.9 15.04 Yes No

IPI00936738.1 Isoform 1 of Torsin-1A-interacting protein 1 TOR1AIP1 66.2 1.95 2.91 Yes No

IPI01013543.1 Triosephosphate isomerase TPI1;TPI1P1 22.9 0 1.72 Yes No

IPI00218320.4 Isoform 3 of Tropomyosin alpha-3 chain TPM3 28.9 5.08 6.55 Yes No

IPI00797490.2

cDNA FLJ53588, highly similar to Translocation-associated

membrane protein 1 TRAM1 39.7 0 2.02 Yes No

IPI00646055.3 Uncharacterized protein TRAP1 74.2 13.35 37.75 Yes No

IPI00181881.11 Uncharacterized protein TRAPPC9 140.6 1.86 1.89 Yes No

IPI00927747.1 T-cell receptor gamma chain V region PT-gamma-1/2 TRGV3 15.7 0 1.65 Yes No

IPI01012915.1

cDNA FLJ53462, highly similar to Transcription

intermediary factor 1-alpha TRIM24 107.6 0 7.41 Yes No

IPI00029629.4 E3 ubiquitin/ISG15 ligase TRIM25 TRIM25 70.9 9.15 8.23 Yes No

IPI00438229.2 Isoform 1 of Transcription intermediary factor 1-beta TRIM28 88.5 22.09 36.46 Yes No

IPI00221114.2 Isoform Beta of E3 ubiquitin-protein ligase TRIM33 TRIM33 120.5 0 1.78 Yes No

IPI00307165.2 Tripartite motif-containing protein 47 TRIM47 69.5 0 2.33 Yes No

IPI00479523.6 Isoform 2 of Triple functional domain protein TRIO 329.2 7.11 19.31 Yes No

IPI00003515.2 Thyroid receptor-interacting protein 11 TRIP11 227.4 0 7.81 Yes No

IPI00003505.3 Isoform 1 of Pachytene checkpoint protein 2 homolog TRIP13 48.5 11.13 16.72 Yes No

IPI00178861.1

Isoform 2 of N(2),N(2)-dimethylguanosine tRNA

methyltransferase TRMT1 69.3 4.19 4 Yes No

IPI00301719.2

Isoform 2 of CCA tRNA nucleotidyltransferase 1,

mitochondrial TRNT1 47.8 0 1.69 Yes No

IPI00219287.1 Isoform Short of 60 kDa SS-A/Ro ribonucleoprotein TROVE2 23.3 2.28 3.11 Yes No

IPI00926412.1

Transformation/transcription domain-associated protein

variant TRRAP 405.6 1.64 1.9 Yes No

IPI00218883.2 Isoform 4 of Tuberin TSC2 198 0 5.79 Yes No

IPI01014105.1 Uncharacterized protein TSG101 40.9 2.47 1.99 Yes No

IPI00292894.5 Pre-rRNA-processing protein TSR1 homolog TSR1 91.8 0 3.13 Yes No

IPI00014361.1 GDP-L-fucose synthase TSTA3 35.9 0 3.53 Yes No

IPI00005634.3 Tetratricopeptide repeat protein 37 TTC37 175.4 8 8.99 Yes No

IPI00000606.5 Tetratricopeptide repeat protein 4 TTC4 44.7 2.75 2.93 Yes No

IPI00011702.4 TEL2-interacting protein 1 homolog TTI1 122 2.61 5.13 Yes No

IPI00640529.2 dual specificity protein kinase TTK isoform 2 TTK 96.9 7.67 7.57 Yes No

IPI00879002.1 Uncharacterized protein TTLL12 73.5 2.28 12.43 Yes No

IPI00879904.1 Isoform 2 of Tubulin alpha chain-like 3 TUBAL3 45.5 6.4 10.61 Yes No

IPI01022236.1 Uncharacterized protein TUBB 46.5 64.58 107.12 Yes No

IPI00013475.1 Tubulin beta-2A chain TUBB2A 49.9 51.82 87.92 Yes No

IPI00646779.2 TUBB6 protein TUBB6 50.1 40.48 62.38 Yes No

SI_II(16)


IPI00930130.2

cDNA FLJ11352 fis, clone HEMBA1000020, highly similar to

Tubulin beta-2C chain TUBB6 44.6 67.06 102.01 Yes No

IPI00295081.1 Tubulin gamma-1 chain TUBG1 51.1 0 4.57 Yes No

IPI00033516.1 Isoform 1 of Gamma-tubulin complex component 3 TUBGCP3 103.5 6.62 6.78 Yes No

IPI00816829.2 Isoform 4 of Twinfilin-1 TWF1 28.8 1.99 7.69 Yes No

IPI00470779.2 Alpha-taxilin TXLNA 61.9 5.68 1.85 Yes No

IPI00019994.4 Isoform 1 of Gamma-taxilin TXLNG 60.5 4.04 3.81 Yes No

IPI00939560.1 thioredoxin domain-containing protein 5 isoform 3

TXNDC5;MUTED;M

UTED-TXNDC5 36.2 1.73 4.96 Yes No

IPI00983068.1 Isoform 1 of Thioredoxin reductase 1, cytoplasmic TXNRD1 70.8 5.98 12.05 Yes No

IPI00830039.1 Isoform 2 of Splicing factor U2AF 65 kDa subunit U2AF2 53.1 0 2.51 Yes No

IPI00829908.1

Isoform 2 of U2 snRNP-associated SURP motif-containing

protein U2SURP 118.2 2.32 10.07 Yes No

IPI00477962.3

Isoform 1 of UDP-N-acetylhexosamine pyrophosphorylase-

like protein 1 UAP1L1 57 5.3 7.38 Yes No

IPI00023234.3 SUMO-activating enzyme subunit 2 UBA2 71.2 6.79 5.2 Yes No

IPI00456429.3 Ubiquitin-60S ribosomal protein L40 UBA52 14.7 6.06 10.78 Yes No

IPI00023647.4 Isoform 1 of Ubiquitin-like modifier-activating enzyme 6 UBA6 117.9 4.41 9.22 Yes No

IPI00171127.1 Ubiquitin-associated protein 2 UBAP2 117 5.19 6.18 Yes No

IPI00946257.1 Isoform 4 of Ubiquitin-associated protein 2-like UBAP2L 103.1 5.53 0 Yes No

IPI00783378.3 Ubiquitin-conjugating enzyme E2 O UBE2O 141.2 0 6.45 Yes No

IPI00604464.3 Isoform 1 of Ubiquitin-protein ligase E3C UBE3C 123.8 0 1.77 Yes No

IPI00655534.3 Isoform 3 of Ubiquitin conjugation factor E4 B UBE4B 121.7 6.94 1.68 Yes No

IPI00927195.1 Uncharacterized protein UBP1 18 0 1.98 Yes No

IPI00646605.3 Isoform 3 of E3 ubiquitin-protein ligase UBR4 UBR4 571.5 13.63 33.58 Yes No

IPI00976576.1 309 kDa protein UBR5 309 2.08 2.41 Yes No

IPI01014777.1

cDNA FLJ53007, highly similar to UBA/UBX 33.3 kDa

protein UBXN1 22.9 3.26 1.73 Yes No

IPI00219512.2

Isoform 2 of Ubiquitin carboxyl-terminal hydrolase

isozyme L5 UCHL5 36.1 5.24 2.24 Yes No

IPI00847664.1 Isoform 3 of Uridine-cytidine kinase-like 1 UCKL1 44.5 0 2.38 Yes No

IPI00827694.1 Isoform 4 of Ubiquitin-conjugating enzyme E2 variant 3 UEVLD 37.6 0 3.96 Yes No

IPI00654779.1 Uncharacterized protein UFD1L 33.3 2.93 0 Yes No

IPI00844000.1 Isoform 1 of E3 UFM1-protein ligase 1 UFL1 89.5 0 11.29 Yes No

IPI00956601.1 UDP-glucose 6-dehydrogenase isoform 2 UGDH 47.6 14.42 14.19 Yes No

IPI00619903.3

Isoform 2 of UDP-glucose:glycoprotein glucosyltransferase

1 UGGT1 174.9 0 4.21 Yes No

IPI00024467.3 UDP-glucose:glycoprotein glucosyltransferase 2 UGGT2 174.6 2.75 0 Yes No

IPI00395676.1 Isoform 2 of UTP--glucose-1-phosphate uridylyltransferase UGP2 55.6 0 4.36 Yes No

IPI00797945.1 E3 ubiquitin-protein ligase UHRF1 UHRF1 89.8 0 5.44 Yes No

IPI00289357.5 Serine/threonine-protein kinase ULK1 ULK1 112.6 4.91 3.34 Yes No

IPI00971144.1 Isoform 3 of Serine/threonine-protein kinase ULK3 ULK3 53.2 0 0 Yes No

IPI00003923.1 Isoform 1 of Uridine 5'-monophosphate synthase UMPS 52.2 4.08 19.69 Yes No

IPI00790059.2 cDNA FLJ54883, highly similar to Unc-13 homolog D UNC13D 106.4 0 4.77 Yes No

IPI00735181.2 Isoform 2 of Protein unc-45 homolog A UNC45A 101.6 3.57 11.07 Yes No

IPI00013847.4 Cytochrome b-c1 complex subunit 1, mitochondrial UQCRC1 52.6 8.19 7.78 Yes No

IPI00305383.1 Cytochrome b-c1 complex subunit 2, mitochondrial UQCRC2 48.4 13.63 13.9 Yes No

IPI00028980.2 Unhealthy ribosome biogenesis protein 2 homolog URB2 170.4 0 5.23 Yes No

IPI01015928.1 Uncharacterized protein USO1 106.1 9.6 8.74 Yes No

IPI00941385.2 Isoform 3 of Ubiquitin carboxyl-terminal hydrolase 10 USP10 87.5 0 9.51 Yes No

IPI00184533.1 Ubiquitin carboxyl-terminal hydrolase 11 USP11 109.7 1.89 2.73 Yes No

IPI00640357.1 ubiquitin carboxyl-terminal hydrolase 14 isoform b USP14 52.4 3.61 5.19 Yes No

IPI00796756.3 Ubiquitin carboxyl-terminal hydrolase USP19 145.5 0 3.66 Yes No

IPI00914628.2 Ubiquitin carboxyl-terminal hydrolase USP24 277.2 7.31 3.98 Yes No

IPI00917974.1 Uncharacterized protein USP39 17.5 0 1.68 Yes No

IPI00607554.2 Isoform 1 of Ubiquitin carboxyl-terminal hydrolase 47 USP47 157.2 6.87 2.12 Yes No

IPI00786950.1 Isoform 2 of Ubiquitin carboxyl-terminal hydrolase 48 USP48 112.9 3.56 2.15 Yes No

IPI00375145.1 Isoform Short of Ubiquitin carboxyl-terminal hydrolase 5 USP5 93.2 1.91 6.62 Yes No

IPI00646721.1 Ubiquitin carboxyl-terminal hydrolase USP7 126.2 1.92 0 Yes No

IPI00030915.1 Ubiquitin carboxyl-terminal hydrolase 8 USP8 127.4 1.78 9.15 Yes No

IPI00003964.4

Isoform 2 of Probable ubiquitin carboxyl-terminal

hydrolase FAF-X USP9X 290.3 4.75 26.86 Yes No

IPI00965993.2

cDNA, FLJ95176, Homo sapiens CGI-48 protein (CGI-48),

mRNA UTP18 57.7 0 3.07 Yes No

IPI00004970.5 Small subunit processome component 20 homolog UTP20 318.2 3.56 1.81 Yes No

IPI00009329.2 Utrophin UTRN 394.2 7.95 6.48 Yes No

IPI00000873.3 Valyl-tRNA synthetase VARS 140.4 17.59 41.46 Yes No

IPI00004977.2 Isoform 3 of Guanine nucleotide exchange factor VAV2 VAV2 97 2.17 2.4 Yes No

IPI00291175.7 Isoform 1 of Vinculin VCL 116.6 74.74 104.22 Yes No

IPI00917420.1 30 kDa protein VDAC2 30.3 2.6 8.32 Yes No

IPI00031804.1

Isoform 1 of Voltage-dependent anion-selective channel

protein 3 VDAC3 30.6 2.21 2.89 Yes No

IPI00855826.1

Isoform 2 of Ventricular zone-expressed PH domain-

containing protein homolog 1 VEPH1 89.6 1.67 0 Yes No

IPI00902899.1

cDNA FLJ32139 fis, clone PLACE5000037, highly similar to

Vacuolar protein sorting 11 VPS11 35.7 0 2.78 Yes No

IPI00305438.3

Isoform 1 of Vacuolar protein sorting-associated protein

16 homolog VPS16 94.6 2.04 5.89 Yes No

IPI00001985.2 Vacuolar protein sorting-associated protein 18 homolog VPS18 110.1 0 4.2 Yes No

IPI00411426.3 Vacuolar protein sorting-associated protein 26A VPS26A 38.1 2.66 5.36 Yes No

IPI00795814.1 Uncharacterized protein VPS33A 61.2 0 2.03 Yes No

IPI00018931.6 Vacuolar protein sorting-associated protein 35 VPS35 91.6 4.93 14.07 Yes No

IPI00646971.1

Isoform 2 of Vacuolar protein-sorting-associated protein

36 VPS36 36.9 0 2.19 Yes No

IPI00922387.2 Uncharacterized protein VPS45 61.9 0 2.04 Yes No

IPI00182728.2 Vacuolar protein sorting-associated protein 4B VPS4B 49.3 4 10.28 Yes No

IPI00656021.2

vacuolar protein sorting-associated protein 53 homolog

isoform 1 VPS53 94.3 0 5.11 Yes No

IPI00298971.1 Vitronectin VTN 54.3 0 0 Yes No

IPI00412737.1 tryptophanyl-tRNA synthetase, cytoplasmic isoform b WARS 48.8 6.53 0 Yes No

IPI00472164.2 Wiskott-Aldrich syndrome protein family member 2 WASF2 54.3 2.57 2.58 Yes No

IPI00011676.2 Neural Wiskott-Aldrich syndrome protein WASL 54.8 1.64 2.09 Yes No

IPI00411614.1 WD repeat and HMG-box DNA-binding protein 1 WDHD1 125.9 0 4.42 Yes No

IPI00746165.2 Isoform 1 of WD repeat-containing protein 1 WDR1 66.2 9.97 14.37 Yes No

IPI00412224.2 WD repeat-containing protein 11 WDR11 136.6 1.74 0 Yes No

IPI00304232.1 Ribosome biogenesis protein WDR12 WDR12 47.7 0 4.18 Yes No

IPI00645819.3 Isoform 2 of WD repeat-containing protein 26 WDR26 70.4 0 4.41 Yes No

IPI00009471.1 WD repeat-containing protein 3 WDR3 106 2.27 1.62 Yes No

IPI00169325.1 WD repeat-containing protein 36 WDR36 105.3 0 7 Yes No

IPI00937477.1 WD repeat-containing protein 43 WDR43 74.8 1.7 8.05 Yes No

IPI00797612.1 WD repeat-containing protein 6 WDR6 124.9 0 0 Yes No

IPI00300060.4 WD repeat-containing protein 70 WDR70 73.2 5.02 4.04 Yes No

IPI00974133.1 Uncharacterized protein WLS 14.4 0 2.45 Yes No

IPI00397592.2 Isoform 3 of Serine/threonine-protein kinase WNK1 WNK1 206.5 0 2.92 Yes No

IPI00029107.2 Werner syndrome ATP-dependent helicase WRN 162.4 0 2.79 Yes No

IPI01015328.1 Uncharacterized protein XAB2 99.6 0 1.7 Yes No

IPI00645898.3 X-prolyl aminopeptidase (Aminopeptidase P) 1, soluble XPNPEP1 62.1 1.72 3.11 Yes No

IPI00795212.1 Isoform 2 of Probable Xaa-Pro aminopeptidase 3 XPNPEP3 48.1 1.96 5.48 Yes No

IPI01018709.1 Uncharacterized protein XPO5 94 2.14 0 Yes No

IPI00302458.10 Exportin-7 XPO7 123.8 4.13 9.28 Yes No

IPI00306290.5 Exportin-T XPOT 109.9 6.51 5.61 Yes No

IPI00893062.1

X-ray repair complementing defective repair in Chinese

hamster cells 6 XRCC6 64.2 44.68 64.39 Yes No

IPI00946262.2 Protein XRN1 130.1 0 4.39 Yes No

IPI00788157.1 Isoform 2 of 5'-3' exoribonuclease 2 XRN2 99.9 0 7.8 Yes No

IPI00007074.5 Tyrosyl-tRNA synthetase, cytoplasmic YARS 59.1 18.29 36.27 Yes No

IPI00643807.1 Uncharacterized protein YBX1 23.6 0 2.1 Yes No

IPI00968057.1 Uncharacterized protein YTHDC2 150.8 4.55 1.93 Yes No

SI_II(17)


IPI01012814.1 Uncharacterized protein YTHDF1 55.3 2.33 0 Yes No

IPI00759832.1 Isoform Short of 14-3-3 protein beta/alpha YWHAB 27.8 3.69 6.03 Yes No

IPI00000816.1 Isoform 1 of 14-3-3 protein epsilon YWHAE 29.2 6.08 6.46 Yes No

IPI00796727.1 Uncharacterized protein YWHAQ 17 3.69 8.4 Yes No

IPI01011530.1 Uncharacterized protein YWHAZ 15.7 3.69 10.41 Yes No

IPI00941127.2

cDNA FLJ58036, highly similar to Homo sapiens sterile

alpha motif and leucine zipper containing kinase AZK

(ZAK), transcript variant 2, mRNA ZAK 40.2 1.61 3.43 Yes No

IPI00747417.4 Uncharacterized protein ZBTB40 125.4 0 0 Yes No

IPI00410069.4 Uncharacterized protein ZC3HAV1 114 7.82 5.78 Yes No

IPI00410664.1 Isoform 2 of Palmitoyltransferase ZDHHC13 ZDHHC13 51.5 0 4.12 Yes No

IPI00748303.3 Uncharacterized protein ZFR 115.1 2.8 0 Yes No

IPI00027180.1 CAAX prenyl protease 1 homolog ZMPSTE24 54.8 0 1.68 Yes No

IPI00219759.1 Isoform 2 of Zinc finger protein 207 ZNF207 49.7 4.02 3.23 Yes No

IPI00152420.2 cDNA FLJ59779, highly similar to Zinc finger protein 398 ZNF398 72 0 2.07 Yes No

IPI00844490.2 Isoform 2 of Zinc finger protein 618 ZNF618 95.6 1.94 4.82 Yes No

IPI00888830.1 Zinc finger protein 806 ZNF806 67.8 0 0 Yes No

IPI00914580.2 Zinc finger protein 879 ZNF879 64.5 4.25 0 Yes No

IPI00303331.4 Zinc finger SWIM domain-containing protein 4 ZSWIM4 110.1 0 1.77 Yes No

IPI01015739.1 ZW10 protein ZW10 76.8 5.36 6.33 Yes No

IPI00658086.1 Uncharacterized protein ZYX 19.1 0 2.09 Yes No

IPI00646774.1 51 kDa protein - 51.4 0 2.57 Yes No

IPI00909073.1

cDNA FLJ53752, highly similar to Heat shock 70 kDa

protein 1 - 45.1 2.77 9.08 Yes No

IPI00942539.1 39 kDa protein ACTN4 39 0 2.5 Yes No

IPI00895834.2 Isoform 4 of B-cell scaffold protein with ankyrin repeats BANK1 73.6 1.92 2.79 Yes No

IPI00442534.1 cDNA FLJ27040 fis, clone SLV08542 BPIFA2 13.7 0 0 Yes No

IPI00964192.1 Protein CC2D2A 102.7 0 0 Yes No

IPI00910737.1 cDNA FLJ54022, highly similar to Exportin-2 CSE1L 86 0 4.11 Yes No

IPI00910433.1

cDNA FLJ60675, highly similar to ATP-dependent RNA

helicase DDX3X DDX3X 58.7 0 1.95 Yes No

IPI00217413.3 ATP-dependent RNA helicase DHX29 DHX29 155.1 0 0 Yes No

IPI00644235.1 Uncharacterized protein DMAP1 27.6 0 0 Yes No

IPI00386975.3 Isoform 1B of Desmocollin-1 DSC1 93.8 0 3.07 Yes No

IPI00940393.3 Uncharacterized protein EEF1A1 47.8 0 7.57 Yes No

IPI00555871.1 Fibroblast growth factor 11 variant (Fragment) FGF11 36.4 2.08 2.41 Yes No

IPI00893518.1 Flotillin 1 FLOT1 9.6 0 3.57 Yes No

IPI01009264.2 Uncharacterized protein GABRB1 46.3 0 0 Yes No

IPI00141938.4 histone H2A.V isoform 2 H2AFV 12.1 0 2.51 Yes No

IPI00784295.2 Isoform 1 of Heat shock protein HSP 90-alpha HSP90AA1 84.6 5.02 13.02 Yes No

IPI00003362.3 78 kDa glucose-regulated protein HSPA5 72.3 4.58 7.52 Yes No

IPI00983926.1 Uncharacterized protein HSPA8 14.6 2.77 11.17 Yes No

IPI00426289.1 Isoform 1 of Inositol hexakisphosphate kinase 2 IP6K2 49.2 0 4.4 Yes No

IPI00789324.4 Uncharacterized protein JUP 66.3 27.37 37.3 Yes No

IPI00922792.2 Uncharacterized protein KPNB1 73.2 0 2.75 Yes No

IPI00171196.2 Isoform 3 of Keratin, type I cytoskeletal 13 KRT13 45.8 19.97 29.45 Yes No

IPI01022175.1

cDNA FLJ55805, highly similar to Keratin, type II

cytoskeletal 4 KRT4 51.7 4.67 13.92 Yes No

IPI01026015.1 87 kDa protein KSR1 87.3 0 2.09 Yes No

IPI00884105.2 Lysosome-associated membrane glycoprotein 1 LAMP1 44.9 0 2.17 Yes No

IPI00916562.1 Protein LEPRE1 16.8 0 2.3 Yes No

IPI00789136.2

cDNA FLJ53328, highly similar to Methylcrotonoyl-CoA

carboxylase subunit alpha, mitochondrial MCCC1 52.6 2.86 12.79 Yes No

IPI00982926.1 Protein MGAT4B 16.2 7.82 8.92 Yes No

IPI01011534.1 Uncharacterized protein MRE11A 80.5 4.13 3.64 Yes No

IPI01026216.1 69 kDa protein MTHFD1 69.4 0 3.28 Yes No

IPI00479390.2 Isoform 2 of Myomesin-1 MYOM1 177.6 0 4.99 Yes No

IPI00867653.1 protocadherin-19 isoform b precursor PCDH19 120.9 0 3.27 Yes No

IPI01010638.1 Uncharacterized protein PHGDH 43.2 0 5.45 Yes No

IPI00396145.2 Isoform 1 of 1-phosphatidylinositol-3-phosphate 5-kinase PIKFYVE 237 2.1 5.88 Yes No

IPI00884918.3 Isoform 2 of Polycystic kidney disease protein 1-like 2 PKD1L2 197.3 0 3.77 Yes No

IPI00880064.1 Isoform 4 of Prolyl endopeptidase-like PREPL 73.3 0 0 Yes No

IPI00976934.1 Uncharacterized protein RPL27A 12.5 0 2.81 Yes No


IPI00014938.3 SAP domain-containing ribonucleoprotein SARNP 23.7 0 3.73 Yes No

IPI00657650.2 Uncharacterized protein SERPINB4 24.4 0 2.07 Yes No

IPI00013890.2 Isoform 1 of 14-3-3 protein sigma SFN 27.8 0 2.08 Yes No

IPI00793790.2

cDNA FLJ39250 fis, clone OCBBF2008691, highly similar to

Transcription factor SOX-5 SOX5 63.9 0 4.55 Yes No

IPI01012096.1 Speckle targeted PIP5K1A-regulated poly(A) polymerase TUT1 93.8 0 1.66 Yes No

IPI00031812.3 Nuclease-sensitive element-binding protein 1 YBX1 35.9 0 7.33 Yes No

IPI01016001.1 Zinc finger protein 678 ZNF678 61.4 0 0 Yes No

SI_II(18)

Excel S2_SILAC data_HEK293T.xlsx

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