(Araceae), (Hydrocharitaceae) (Araceae), (Hydrocharitaceae)

ISSN 1346-7565 Acta Phytotax. Geobot. 65 (2): 89–97 (2014)

With its wealth of plant diversity, Myanmar (Burma) constitutes a significant component of the Indo-Malaysian biodiversity hotspot in both land area and species richness (van Dijk et al. 2004). Yet, for a number of reasons, it is one of the most poorly investigated floristic areas in the world, with only five specimens per 100 km2 (Campbell & Hammond 1989). The reported total of some 11,800 species (Kress et al. 2003) may therefore represent only a fraction of the entire flora of the country.

While revising the flora of Myanmar, a de-cade-long inventory of the plants of Myanmar led by Japanese botanists have discovered many new species, and recorded noteworthy plant collec-tions (Tanaka 2005, 2012a, 2012b, Tanaka et al. 2006a, 2006b, 2007, 2009, 2010a, 2010b, 2010c, 2011, Tanaka & Nagamasu 2006, Tanaka & Hughes 2007, Ito et al. 2009, Murata et al. 2010, Yukawa et al. 2010, Tanaka & Hayami 2011, Ito et

al. 2014). Here we add three more aquatic mono-cots to the flora of Myanmar; Lemna trisulca L. (Araceae), Blyxa aubertii var. echinosperma (C.B. Clarke) C.D.K. Cook & Lüönd and Najas tenuis A. Braun ex Magnus (Hydrocharitaceae). We also analyzed chloroplast DNA sequences of all three taxa and counted the chromosomes of N. tenuis to compare with related taxa and/or other regional materials. The importance and charac-teristics of these collections are also provided.

Materials and Methods

Materials of Lemna trisulca (Araceae), Blyxa aubertii var. echinosperma, and Najas tenuis (Hydrocharitaceae) were collected on expedi-tions to Myanmar in 2005 and 2008. The collec-tions were identified using the keys prepared by Cook (1996). The first set of voucher specimens has been deposited in the herbarium of the Forest

New or Noteworthy Plant Collections from Myanmar (8) Lemna trisulca (Araceae), Blyxa aubertii var. echinosperma, and Najas tenuis

(Hydrocharitaceae)

Yu Ito1,†, tetsuo ohI-toma1, NobuYukI taNaka2, NorIo taNaka3 aNd JIN murata1,*

1Botanical Gardens, Graduate School of Sciences, The University of Tokyo, Tokyo, 112-0001 Japan. *[email protected] (author for correspondence); 2Kochi Prefectural

Makino Botanical Garden, Kochi, 781-8125 Japan; 3Tsukuba Botanical Garden, National Museum of Nature and Science, Tsukuba, 305-0005, Japan

†Present address: School of Biological Sciences, University of Canterbury, Christchurch 8020, New Zealand

During floristic research in Myanmar, some noteworthy aquatic monocots new to the flora of Myanmar were collected: Lemna trisulca L. (Araceae), Blyxa aubertii var. echinosperma (C.B.Clarke) C.D.K. Cook & Lüönd, and Najas tenuis A. Braun ex Magnus (Hydrocharitaceae). To compare them with re-lated taxa and/or other regional materials, chloroplast DNA was sequenced for all taxa and the chromo-some of N. tenuis were counted. Distribution ranges are given for each taxon based on a literature review.

Key words: aquatic plants, Blyxa, Lemna, Myanmar, Najas

The Japanese Society for Plant Systematics

NII-Electronic Library Service

The JapaneseSociety foi Plant Systematics

ISSN 1346-7565 Acta Phytotax. Geobot. 65 (2): 89-97 (2014)

New or Noteworthy Plant Collections from Myanmar (8) Lemna trisutea

(Araceae), Blyxa aubertii vaL eehinospeitmta, and Nojas tenuis

(Hydrocharitaceae)

Yu ITo'･', TETsuo OHI:I"oMA', NoBuyuKI TANAKA2, AND JIN MuRATAI･'

3NORIO TANAKA

'Botanicat

Gardens, Graduate Schoot ofSciences, T7ie Uhiversity of7bkyo, 7bkyo, IJ2-OOOI JZrpan.

'[email protected],o.ac.Xpkiuthorforcorrespondencoj;?KbchiPrajlicturat

Mdkino Botanical Garden, Kbchi, 781-8i25 .iapan; 3kukuba

Botanicat Garden, iVlationat

Museum qfNlrture andScience, kukuba, 305-OO05, .iapan'Present address.' School ofBiological Sciences, Uhiversity ofCZinterbur.i,, Christchurch 8020, Nlew Zealand

During fioristic research in Myanmar, some noteworthy aquatic monocots new to the fiora ofMyanmar

were collected: Lemna trisulca L. (Araceae), B4}ixa aubertii var. echinosperma (C.B.Clarke) C.D.K.Cook & LUOnd. and Nby'as tenuis A. Braun ex Magnus (Hydrocharitaceae). Tb compare them with re-

lated taxa andlor other regional materials, chloroplast DNA was sequenced for all taxa and the chromo-

some ofN. tenuis were counted. Distribution ranges are given tbr each taxon based on a literature review.

Key words: aquatic plants. B4i,xa. Lemna, Myanmar. Nby'as

With its wealth of plant diversity, Myanmar

(Burmaj constitutes a significant component of

the Indo-Malaysian biodiversity hotspot in bothland area and species richness (van Dijk et aL

2004), Yet, fora number of reasons, it is one of

the most poorly investigated fioristic areas in theworld, with only five specimens per 100 km2

(Campbell & Hammond 1989), The reported total

of some 11,800 species (Kress et al, 2003) may

therefore represent only a fraction of the entire

fiora of the country,

While revising the fiora of Myanmar, a de-

cade-longinventoryoftheplantsofMyanmarled

by Japanese botanists have discovered many new

species, and recorded noteworthy plant collec-

tions (Tanaka 2005, 2012a, 2012b, [lanaka et aL

2006a, 2006b, 2007, 2009, 2010a, 2010b, 2010c,2011, [lanaka & Nagamasu 2006, [lanaka &Hughes 200Z Ito et al, 2009, Murata et aL 2010,

YUkawa etal, 201O, [lanaka & Hayami 2011, Ito et

al, 2014), Here we add three more aquatic mono-

cots to the fiora of Myanmar; Lemna trisulca L,

(Araceae), BCyxa aubertii var, echinosperma

(C,B, Clatke) C,D,K, Cook & LUOnd and IVIijas

tenuis A, Braun ex Magnus (Hydrocharitaceae),We also analyzed chloroplast DNA sequences of

all three taxa and counted the chromosomes oflV/

tenuis to compare with related taxa and/or other

regional materials, The importance and charac-

teristics of these collections are also provided,


Materials of Lemna trisulca (Araceae), Blyxa

aubertii var, echinosperma, and IVIijas tenuis

(Hydrocharitaceae) were collected on expedi-

tions to Myanmar in 2005 and 2008, The collec-

tions were identified using the keys prepared byCook (1996), The first set ofvoucher specimens

has been deposited in the herbarium of the Forest

NII-Electronic


ISSN 1346-7565 Acta Phytotax. Geobot. 65 (2): 89-97 (2014)

New or Noteworthy Plant Collections from Myanmar (8) Lemna trisutea

(Araceae), Blyxa aubertii vaL eehinospeitmta, and Nojas tenuis

(Hydrocharitaceae)

Yu ITo'･', TETsuo OHI:I"oMA', NoBuyuKI TANAKA2, AND JIN MuRATAI･'

3NORIO TANAKA

'Botanicat

Gardens, Graduate Schoot ofSciences, T7ie Uhiversity of7bkyo, 7bkyo, IJ2-OOOI JZrpan.

'[email protected],o.ac.Xpkiuthorforcorrespondencoj;?KbchiPrajlicturat

Mdkino Botanical Garden, Kbchi, 781-8i25 .iapan; 3kukuba

Botanicat Garden, iVlationat

Museum qfNlrture andScience, kukuba, 305-OO05, .iapan'Present address.' School ofBiological Sciences, Uhiversity ofCZinterbur.i,, Christchurch 8020, Nlew Zealand

During fioristic research in Myanmar, some noteworthy aquatic monocots new to the fiora ofMyanmar

were collected: Lemna trisulca L. (Araceae), B4}ixa aubertii var. echinosperma (C.B.Clarke) C.D.K.Cook & LUOnd. and Nby'as tenuis A. Braun ex Magnus (Hydrocharitaceae). Tb compare them with re-

lated taxa andlor other regional materials, chloroplast DNA was sequenced for all taxa and the chromo-

some ofN. tenuis were counted. Distribution ranges are given tbr each taxon based on a literature review.

Key words: aquatic plants. B4i,xa. Lemna, Myanmar. Nby'as

With its wealth of plant diversity, Myanmar

(Burmaj constitutes a significant component of

the Indo-Malaysian biodiversity hotspot in bothland area and species richness (van Dijk et aL

2004), Yet, fora number of reasons, it is one of

the most poorly investigated fioristic areas in theworld, with only five specimens per 100 km2

(Campbell & Hammond 1989), The reported total

of some 11,800 species (Kress et al, 2003) may

therefore represent only a fraction of the entire

fiora of the country,

While revising the fiora of Myanmar, a de-

cade-longinventoryoftheplantsofMyanmarled

by Japanese botanists have discovered many new

species, and recorded noteworthy plant collec-

tions (Tanaka 2005, 2012a, 2012b, [lanaka et aL

2006a, 2006b, 2007, 2009, 2010a, 2010b, 2010c,2011, [lanaka & Nagamasu 2006, [lanaka &Hughes 200Z Ito et al, 2009, Murata et aL 2010,

YUkawa etal, 201O, [lanaka & Hayami 2011, Ito et

al, 2014), Here we add three more aquatic mono-

cots to the fiora of Myanmar; Lemna trisulca L,

(Araceae), BCyxa aubertii var, echinosperma

(C,B, Clatke) C,D,K, Cook & LUOnd and IVIijas

tenuis A, Braun ex Magnus (Hydrocharitaceae),We also analyzed chloroplast DNA sequences of

all three taxa and counted the chromosomes oflV/

tenuis to compare with related taxa and/or other

regional materials, The importance and charac-

teristics of these collections are also provided,


Materials of Lemna trisulca (Araceae), Blyxa

aubertii var, echinosperma, and IVIijas tenuis

(Hydrocharitaceae) were collected on expedi-

tions to Myanmar in 2005 and 2008, The collec-

tions were identified using the keys prepared byCook (1996), The first set ofvoucher specimens

has been deposited in the herbarium of the Forest

NII-Electronic

90 Vol. 65Acta Phytotax. Geobot.

Department, Ministry of Environmental Conser-vation and Forestry, Union of Myanmar (RAF). Duplicates are deposited in the herbaria of the Makino Botanical Garden (MBK) and the Uni-versity of Tokyo (TI).

Molecular comparisons were made using two slightly different strategies. For Blyxa aubertii var. echinosperma and Lemna trisulca, for which DNA sequences were already available in Gen-Bank, we followed the latest DNA barcoding methods (CBOL Plant Working Group 2009) and chose the chloroplast DNA sequences of rbcL and matK as molecular markers. For Najas tenuis, the more informative psbA–trnH was also analyzed in addition to the above two markers, to enable further elucidation of its relationships. The se-quences were obtained using the procedure of Ito et al. (2010). Each target region was amplified by polymerase chain reaction (PCR) using the fol-lowing primer pairs: rbcL Z1 (Wolf et al. 1994) and either rbcL 636R (5'-AATAMGAAMCGAT-CACTCCAACG) modified from Pryer et al. (2004) or rbcL 1379R (Little & Barrington 2003) for rbcL; RM_749F (Ito et al. 2010) and either 8R (Ooi et al. 1995) or 1520R (Whitten et al. 2000) for matK. The obtained sequences were then ap-plied for BLAST search to find comparable se-quences (Altschul et al. 1990, Altschul et al. 1997); those with a 99 % match or closer were ob-tained for comparison (Tables 1 and 2). Align-ments were performed after trimming redundant nucleotides. The final lengths of the alignments were as follows: 606 bp and 542 bp (rbcL and matK of B. aubertii), 1140 bp, 721 bp, and 303 bp (rbcL, matK, and psbA–trnH of Najas), and 606 bp and 510 bp (rbcL and matK of L. trisulca), re-spectively. The sequences obtained in the present study were registered with the DNA Data Bank of Japan (DDBJ), which is linked to GenBank. Their accession numbers are given below (Tables 1 and 2).

For Najas, in which polyploidy is widely known (e.g., You et al. 1985), we observed the chromosomes of Najas tenuis using the proce-dure of Ito et al. (2010).

To determine the geographic range of each species we searched the publications by den Har-

tog (1957), Dandy (1978), Leach & Osborne (1985), Wang & Sun (1992), Kadono (1994), Cook (1996), Choi (2000), and Hayens (2001).

Results

ARACEAELemna trisulca L. in Sp. Pl. 2: 970. 1753. —Fig. 1

Voucher specimens. Myanmar, Shan State, Inlay Lake, Nyaung Shwe Township, 20°32′02″N, 96°53′53″E. 3 Dec. 2008, N. Tanaka & al. 080649 (MBK, RAF, TI).

Distribution. Bangladesh, N, W, and S China, N and E India, Indonesia (Sumatra, New Guinea), Japan, Malaysia, Myanmar, Papua New Guinea, Pakistan, Philippines; Europe; Oceania; N. America; S. America.

The recovered rbcL sequence from the Myan-mar collection was identical to sequences from plants in the UK (JN894635, JN895273, JN895559, JN895561), USA (GU454430), Canada (GU454429, HQ901580), Australia (AY034237), and unknown (GU454431). In comparing matK, two lineages of Lemna trisulca were revealed, one from Canada and the USA and the other from the UK and Australia, plus the unknown (Table 1).

HYDROCHARITACEAEBlyxa aubertii Rich. var. echinosperma (C.B. Clarke) C.D.K. Cook & Lüönd in Aquat. Bot. 15: 14, f. 3e–h. 1983. —Fig. 2

table 1. Nucleotide sequence variation in rbcL and matK re-gion of chloroplast DNA in Lemna trisulca.

TaxonAccession Number

LocalitymatK

8 247

389

rbcL matKL. trisulca GU454431 GU454169 Unknown G T TL. trisulca AY034237 AY034199 Australia G T TL. trisulca GU454429 GU454167 Canada T T GL. trisulca HQ901580 N/A CanadaL. trisulca JN892881 JN894635 UK G T TL. trisulca JN892881 JN895273 UK G G TL. trisulca JN892881 JN895559 UK G T TL. trisulca JN892881 JN895561 UK G T TL. trisulca GU454430 GU454168 USA T T GL. trisulca AB787656* AB787657* Myanmar G T T*The sequence obtained in the present study.




90 Acta Phytotax. Geobot. Vol. 65

Department, Ministry of Environmental Conser-

vation and Forestry, Union of Myanmar (RAF),Duplicates are deposited in the herbaria of the

Makino Botanical Garden (MBK) and the Uni-

versity of Ibkyo (TI), Molecular comparisons were made using two

slightly different strategies, For B(yxa aubertii

var, echinosperma and Lemna trisulca, fbr whichDNA sequences were already available in Gen-Bank, we fbllowed the latest DNA barcodingmethods (CBOL PIant Wbrking Group 2009) and

chose the chloroplast DNA sequences of rb cL and

matK as molecular markers, For Nke'as tenuis, themore infbrmative psbA-trnH was also analyzed

in addition to the above two markers, to enable

further elucidation of its relationships, The se-

quences were obtained using the procedure ofIto

et al, (2010), Each target region was amplified by

polymerase chain reaction (PCR) using the fbl-

lowing primer pairs: rbcL ZI (Wolf et aL 1994)

and either rbcL 636R (5'-AATAMGAAMCGAT-CACTCCAACG) modified fi;om Pryer et aL

(2004) or rbcL 1379R (Little & Barrington 2003)

fbr rbcL; RM-749F (Ito et al, 2010) and either 8R

(Ooi et al, 1995) or 1520R (Whitten et aL 2000)fbr mati<, The obtained sequences were then ap-

plied for BLAST search to find comparable se-

quences (Altschul et al, 1990, Altschul et al,

1997); those with a 99 % match or closer were ob-

tained fbr comparison (Tables 1 and 2), Align-ments were perfbrmed after trimming redundant

nucleotides, The final lengths of the alignments

were as fbllows: 606 bp and 542 bp (rbcL and

mati( ofB. aubertii), 1140 bp, 721 bp, and 303 bp

(rbcL, mati<, and psbA-trnH of IVby'as), and 606bp and 510 bp (rbcL and matK ofL. trisulca), re-

spectively, The sequences obtained in the presentstudy were registered with the DNA Data Bank of

Japan (DDBJ), which is linked to GenBank,Their accession numbers are given below (Tables1 and 2),

For IVIe'as, in which polyploidy is widely

known (e,g,, Ybu et al, 1985), we observed the

chromosomes of IVIe'as tenuis using the proce-dure of Ito et al, (2010), [Ib determine the geographic range of each

species we searched the publications by den Har-

TABLEI.Nucleotidesequencevariationint'bcLandmatKre-

gionofchloroplastDNAinLemnan'isulca.

TaxonAccessionNumber

rbcLtitatK

mati(

Locality . F a pt th

L.tristtlca GU454431 GU454169L.tristtlca AY034237 AY034199

L.tristtlca GU454429 GU454167

L.tristtlca HQ901580 NtA

L. tristtlca JN8928gl JNg94635

L. t"isttlca JN8928gl JNg95273


L. tristtlca JN8928gl JNg95561L.tristtlca GU454430 GU45416gL.tristtlca AB787656' AB787657'

UnknownAustraliaCanadaCanadaUKUKUKUKUSAM

anmar

GTTGTTTTGGTTGGTGTTGTTTTGGTT

*Thesequenceobtainedinthepresentstudy.

tog (1957), Dandy (1978), Leach & Osborne

(1985), Wang & Sun (1992), Kadono (1994), Cook

(1996), Choi (2000), and Hayens (2001),

Results

ARACEAELemna trisulcaL, in Sp, Pl, 2: 970, 1753, -Fig, 1

Ploucher specimens. Myanmar. Shan State. InlayLake. Nyaung Shwe Tbwnship, 20032'02"N. 96053'53"E.

3 Dec. 2008. N. 7lrnaka & aL 080649 (MBK. RAF. TI).

Distribution, Bangladesh, N, Wl and S China,N and E India, Indonesia (Sumatra, New Guinea),Japan, Malaysia, Myanmar, Papua New Guinea,Pakistan, Philippines; Europe; Oceania; N,

America; S, America,

The recovered rbcL sequence firom the Myan-mar collection was identical to sequences from

plants in the UK (JN894635, JN895273,

JN895559, JN895561), USA (GU454430), Canada

(GU454429, HQ901580), Australia (AY034237),and unknown (GU454431), In comparing matK,

two lineages of Lemna trisulca were revealed,

one from Canada and the USA and the other fromthe UK and Australia, plus the unknown ([lable1),

HYDROCHARITACEAEBIyxa aubertii Rich, var, echinosperma (C,B,Clarke) C,D,K, Cook & LUOnd in Aquat, Bot, 15:14, t 3e-h, 1983, -Fig, 2



Department, Ministry of Environmental Conser-

vation and Forestry, Union of Myanmar (RAF),Duplicates are deposited in the herbaria of the

Makino Botanical Garden (MBK) and the Uni-

versity of Ibkyo (TI), Molecular comparisons were made using two

slightly different strategies, For B(yxa aubertii

var, echinosperma and Lemna trisulca, fbr whichDNA sequences were already available in Gen-Bank, we fbllowed the latest DNA barcodingmethods (CBOL PIant Wbrking Group 2009) and

chose the chloroplast DNA sequences of rb cL and

matK as molecular markers, For Nke'as tenuis, themore infbrmative psbA-trnH was also analyzed

in addition to the above two markers, to enable

further elucidation of its relationships, The se-

quences were obtained using the procedure ofIto

et al, (2010), Each target region was amplified by

polymerase chain reaction (PCR) using the fbl-

lowing primer pairs: rbcL ZI (Wolf et aL 1994)

and either rbcL 636R (5'-AATAMGAAMCGAT-CACTCCAACG) modified fi;om Pryer et aL

(2004) or rbcL 1379R (Little & Barrington 2003)

fbr rbcL; RM-749F (Ito et al, 2010) and either 8R

(Ooi et al, 1995) or 1520R (Whitten et aL 2000)fbr mati<, The obtained sequences were then ap-

plied for BLAST search to find comparable se-

quences (Altschul et al, 1990, Altschul et al,

1997); those with a 99 % match or closer were ob-

tained fbr comparison (Tables 1 and 2), Align-ments were perfbrmed after trimming redundant

nucleotides, The final lengths of the alignments

were as fbllows: 606 bp and 542 bp (rbcL and

mati( ofB. aubertii), 1140 bp, 721 bp, and 303 bp

(rbcL, mati<, and psbA-trnH of IVby'as), and 606bp and 510 bp (rbcL and matK ofL. trisulca), re-

spectively, The sequences obtained in the presentstudy were registered with the DNA Data Bank of

Japan (DDBJ), which is linked to GenBank,Their accession numbers are given below (Tables1 and 2),

For IVIe'as, in which polyploidy is widely

known (e,g,, Ybu et al, 1985), we observed the

chromosomes of IVIe'as tenuis using the proce-dure of Ito et al, (2010), [Ib determine the geographic range of each

species we searched the publications by den Har-

TABLEI.Nucleotidesequencevariationint'bcLandmatKre-

gionofchloroplastDNAinLemnan'isulca.

TaxonAccessionNumber

rbcLtitatK

mati(

Locality . F a pt th

L.tristtlca GU454431 GU454169L.tristtlca AY034237 AY034199

L.tristtlca GU454429 GU454167

L.tristtlca HQ901580 NtA


L. t"isttlca JN8928gl JNg95273


L. tristtlca JN8928gl JNg95561L.tristtlca GU454430 GU45416gL.tristtlca AB787656' AB787657'

UnknownAustraliaCanadaCanadaUKUKUKUKUSAM

anmar

GTTGTTTTGGTTGGTGTTGTTTTGGTT

*Thesequenceobtainedinthepresentstudy.

tog (1957), Dandy (1978), Leach & Osborne

(1985), Wang & Sun (1992), Kadono (1994), Cook

(1996), Choi (2000), and Hayens (2001),

Results

ARACEAELemna trisulcaL, in Sp, Pl, 2: 970, 1753, -Fig, 1

Ploucher specimens. Myanmar. Shan State. InlayLake. Nyaung Shwe Tbwnship, 20032'02"N. 96053'53"E.

3 Dec. 2008. N. 7lrnaka & aL 080649 (MBK. RAF. TI).

Distribution, Bangladesh, N, Wl and S China,N and E India, Indonesia (Sumatra, New Guinea),Japan, Malaysia, Myanmar, Papua New Guinea,Pakistan, Philippines; Europe; Oceania; N,

America; S, America,

The recovered rbcL sequence firom the Myan-mar collection was identical to sequences from

plants in the UK (JN894635, JN895273,

JN895559, JN895561), USA (GU454430), Canada

(GU454429, HQ901580), Australia (AY034237),and unknown (GU454431), In comparing matK,

two lineages of Lemna trisulca were revealed,

one from Canada and the USA and the other fromthe UK and Australia, plus the unknown ([lable1),

HYDROCHARITACEAEBIyxa aubertii Rich, var, echinosperma (C,B,Clarke) C,D,K, Cook & LUOnd in Aquat, Bot, 15:14, t 3e-h, 1983, -Fig, 2

June 2014 91Ito & al.—New or Noteworthy Plant Collections from Myanmar (8)

FIg. 1. Voucher specimen of Lemna trisulca.



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FIg. 2. Voucher specimen of Blyxa aubertii var. echinosperma.



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Voucher specimen. Myanmar, Kachin State, 26°40′50″N, 96°15′20″E, in deep lake, 10 Dec. 2005, J. Murata & al. 040940 (MBK, RAF, TI).

Distribution. Bangladesh, C and S China, W, C, and S India, Indonesia (Java), Japan, Malaysia (Borneo, Peninsular), Myanmar, Nepal, Papua New Guinea, Philippines, Thailand, Sri Lanka; Oceania.

Blyxa aubertii var. echinosperma is distin-guished from var. aubertii, also known from Myanmar (Kress et al. 2003), by the numerous clear spines on the seed surface and long tails on the edges of the seeds (Fig. 3; Cook & Lüönd 1983). Although the varieties have occasionally been treated as distinct species (e.g., Kadono 1994), given the uniform rbcL sequences of B. aubertii var. echinosperma from Myanmar (AB787654) and Japan (AB088810; including six ambiguous sequence sites) and B. aubertii var. aubertii from the USA (rbcL: U80694), we treat

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FIg. 3. Fruit morphology of Blyxa aubertii var. echinosper-ma. Scale bar = 1 mm.



The 　Japanese 　Society 　for 　Plant 　Syst 二ematics

93Ito＆ AL ．− New 　or 　Noteworthy 　Plant　Collections　from　Myanmar （8）June　2014

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　　VJuche厂 speci〃z8’1．　Myanlnar，　Kachin　State，26°40厂50厂厂N，

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ambiguous 　sequence 　sites）and 　B ．　 aubertii 　var ．α わertii　from　the　USA （酌 c五：U80694），　we 　treat

N 工工一Electronic 　 Library 　 Ser ▽

The 　Japanese 　Society 　for 　Plant 　Syst 二ematics

93Ito＆ AL ．− New 　or 　Noteworthy 　Plant　Collections　from　Myanmar （8）June　2014

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　　　 η 卍α ．Scale　bar＝lmm ．

　　VJuche厂 speci〃z8’1．　Myanlnar，　Kachin　State，26°40厂50厂厂N，

96°15厂20厂厂E，in　deep　lake，10　Dec ．2005 ，　J 　Murata ＆ α！．040940（MBK ，　RAF ，　TI）．

　　ヱ）istribution．　Bangladesh，　C　and 　S　China，　W ，C，and 　S　India，　Indonesia（Java），　Japan，　Malaysia

（Borneo，　Peninsular），　Myanmar，　Nepal，　PapuaNew 　Guinea，　Philippines，　Thailand，　Sri　Lanka ；

Oceania，

　　Blyxa　aubertii 　var ．　 echinosper 〃za 　is　distin−

guished　 from　var ．　 aubertii ，　also　known 　fromMyanmar （Kress　et　al．2003），　by　the　numerous

clear 　spines 　on 　the　seed 　surface 　and 　long　tails　on

the　edges 　of 　the　seeds （Fig．3；Cook ＆ LU6nd

1983）．Although 　the　varieties　have　occasionallybeen　treated　as　distinct　species （e．g．，　Kadono1994），given　the　unifbrm 　rbcL 　sequences 　of 　B ．

aubertii 　 var．　 echinosperma 　from　Myanmar

（AB787654）and 　Japan（ABO88810 ；including　six

ambiguous 　sequence 　sites）and 　B ．　 aubertii 　var ．α わertii　from　the　USA （酌 c五：U80694），　we 　treat

N 工工一Electronic 　 Library 　 Ser ▽


FIg. 4 . Voucher specimen of Najas tenuis.



TheJapaneseSocietyforPlantSystematics

94F-

"V"sUniye +e

DeL xu

A

Acta Phytotax. Geobot.t.-"

. T

Vol. 65

MoraofMyallmarMVANMsaJAeiVUStsoeoranATInmoGm--laelD-4d--/,--lhoop+hlEuiutHEV--H AS-na1[udHsim--vdTdyop"/-

1

!

FIG.4. Voucher specimen of IVbjas tenuis.

N-'mdeee e rvde

ix/RfYANMAR:SHANState/kilnyLalanyNymmgShwt Torvmhip.

2or 12' uz'rv/g6e s3'sa'E

Notes,Heib,glowdginwntct,cH.1.Smindcpth.

VtuclterforDNAmulysis.

an,NobuyuhiTthTllatShiSugawam,AliycNdia

YuItolhtet3beetuimnms

No. oorut42gk

NII-Electrontc Library

TheJapaneseSocietyforPlantSystematics

94F-

"V"sUniye +e

DeL xu

A

Acta Phytotax. Geobot.t.-"

. T

Vol. 65

MoraofMyallmarMVANMsaJAeiVUStsoeoranATInmoGm--laelD-4d--/,--lhoop+hlEuiutHEV--H AS-na1[udHsim--vdTdyop"/-

1

!

FIG.4. Voucher specimen of IVbjas tenuis.

N-'mdeee e rvde

ix/RfYANMAR:SHANState/kilnyLalanyNymmgShwt Torvmhip.

2or 12' uz'rv/g6e s3'sa'E

Notes,Heib,glowdginwntct,cH.1.Smindcpth.

VtuclterforDNAmulysis.

an,NobuyuhiTthTllatShiSugawam,AliycNdia

YuItolhtet3beetuimnms

No. oorut42gk

NII-Electrontc Library


close relationship with N. minor (Fig. 5; Table 2; 2n = 24: China: You et al. 1985, You 1989; Russia: Davenport 1980). N. indica, under which N. tenuis is often synonymized (e.g., Rendle 1899), was determined to be unrelated (97% or less sim-ilarity in psbA–trnH). The square to hexagonal and somewhat longer than broad seed coat mor-phology is unique in N. tenuis (Fig. 6). In N. mi-nor the seed coat is characterized by broader than

FIg. 5. Somatic chromosomes of Najas tenuis. Scale bar = 5 μm.

FIg. 6. Seed coat morphology of Najas tenuis. A. Whole seed. Scale bar = 0.5 mm; B. Surface pattern of seed coat. Scale bar = 100 μm.

them as varieties. Further genetic comparisons of matK revealed no genetic differences between specimens from Myanmar (AB787655) and Japan (AB088781). A morphologically similar species, Blyxa quadricostata Hartog, is known from Myanmar and Thailand (Cook 1996, Haynes 2001). The unisexual fl owers, spathes with more than 2 fl owers, fl owers with 6–9 stamens, and ob-tuse and hooded/boat-shaped leaf tips are unique to B. quadricostata (Cook 1996, Haynes 2001).

Najas tenuis A. Braun ex Magnus in Beitr. Ken-ntn. Najas: 7. 1870. —Fig. 4

Voucher specimens. Myanmar, Shan State, Inlay Lake, Nyaung Shwe Township, 20°32' 02"N, 96°53' 53"E. 3 Dec. 2008, N. Tanaka & al. 080642 (MBK, RAF, TI).

Distribution. C and S India, Myanmar, Sri Lanka.

Chromosome number. 2n = 24 (Fig. 5)Najas tenuis has been reported from a small

portion of tropical Asia, including India and Sri Lanka. A chromosome number, 2n = 24, as well as chloroplast DNA sequence data, implies a



TheJapaneseSociety foi Plant Systematics

June 2014 Ito & AL.-New or Noteworthy Plant Collections from Myanmar (8) 95

them as varieties, Further genetic comparisons of

matl( revealed no genetic differences betweenspecimens from Myanmar (AB787655) and Japan

(AB088781), A morphologically similar species,

Blyxa quadricostata Hartog, is known fromMyanmar and Thailand (Cook 1996, Haynes2001), The unisexual fiowers, spathes with more

than 2 fiowers, fiowers with 6-9 stamens, and ob-

tuse and hoodedfboat-shaped leaftips are unique

to B. quadricostata (Cook 1996, Haynes 2001),

close relationship with IVI minor (Fig, 5; Table 2;

2n =24: China: Ybu et aL 1985, Ybu 1989; Russia:Davenport 1980), IV/ indica, under which IV/tenuis is often synonymized (e,g,, Rendle 1899),

was determined to be unrelated (9796 or less sim-ilarity in psbA-trnH), The square to hexagonaland somewhat longer than broad seed coat moF

phology is unique in AC tenuis (Fig, 6), In IV/ mi-nor the seed coat is characterized by broader than

Najas tenuis A, Braun ex Magnus in Beitr, Ken-ntn, Najas: 7, 1870, -Fig, 4

Ploucher specimens. Myanmar. Shan State. Inlay

Lake,NyaungShweTbwnship.20032'02"N.96053'53"E.3 Dec. 2008. N. 1lanaka & al. 080642 (MBK, RAF. TI).

Distribution, C and S India, Myanmar, SriLanka,

Chromosome numben 2n = 24 (Fig, 5)

IVdjas tenuis has been reported from a small

portion of tropical Asia, including India and SriLanka, A chromosome number, 2n

= 24, as well

as chloroplast DNA sequence data, implies a

-

F[G. 5. Somatic chromosomes of M{i'as tenuis. Scale bar= 5

pm.

F]G. 6. Seed coat morphology ofN4i'as tenuis. A. Whole seed. Scale bar= O.5 mm; B. Surface pattern of seed coat. Scale bar

=

100 pm.

NII-Electrontc


June 2014 Ito & AL.-New or Noteworthy Plant Collections from Myanmar (8) 95

them as varieties, Further genetic comparisons of

matl( revealed no genetic differences betweenspecimens from Myanmar (AB787655) and Japan

(AB088781), A morphologically similar species,

Blyxa quadricostata Hartog, is known fromMyanmar and Thailand (Cook 1996, Haynes2001), The unisexual fiowers, spathes with more

than 2 fiowers, fiowers with 6-9 stamens, and ob-

tuse and hoodedfboat-shaped leaftips are unique

to B. quadricostata (Cook 1996, Haynes 2001),

close relationship with IVI minor (Fig, 5; Table 2;

2n =24: China: Ybu et aL 1985, Ybu 1989; Russia:Davenport 1980), IV/ indica, under which IV/tenuis is often synonymized (e,g,, Rendle 1899),

was determined to be unrelated (9796 or less sim-ilarity in psbA-trnH), The square to hexagonaland somewhat longer than broad seed coat moF

phology is unique in AC tenuis (Fig, 6), In IV/ mi-nor the seed coat is characterized by broader than

Najas tenuis A, Braun ex Magnus in Beitr, Ken-ntn, Najas: 7, 1870, -Fig, 4

Ploucher specimens. Myanmar. Shan State. Inlay

Lake,NyaungShweTbwnship.20032'02"N.96053'53"E.3 Dec. 2008. N. 1lanaka & al. 080642 (MBK, RAF. TI).

Distribution, C and S India, Myanmar, SriLanka,

Chromosome numben 2n = 24 (Fig, 5)

IVdjas tenuis has been reported from a small

portion of tropical Asia, including India and SriLanka, A chromosome number, 2n

= 24, as well

as chloroplast DNA sequence data, implies a

-

F[G. 5. Somatic chromosomes of M{i'as tenuis. Scale bar= 5

pm.

F]G. 6. Seed coat morphology ofN4i'as tenuis. A. Whole seed. Scale bar= O.5 mm; B. Surface pattern of seed coat. Scale bar

=

100 pm.

NII-Electrontc


LXVII. Taxon 29: 351.den Hartog C. 1957. Hydrocharitaceae. In: van Steenis, C.

G. G. J. (ed.), Flora Malesiana ser. 1. 5: 381–413. Noordhoff, Leyden.

Hayens, R. R. 2001. Hydrocharitaceae. In: Santisuk, T. and K. Larsen (eds.), Flora of Thailand 7, pp. 365–382. The Forest Herbarium, Royal Forest Depart-ment, Bangkok.

Ito, Y., T. Ohi-Toma, J. Murata & Nr. Tanaka. 2010. Hy-bridization and polyploidy of an aquatic plant, Rup-pia (Ruppiaceae), inferred from plastid and nuclear DNA phylogenies. Amer. J. Bot. 97: 1156–1167.

Ito, Y., Nb. Tanaka, T. Ohi-Toma & J. Murata. 2009. New or noteworthy plant collection from Myanmar (3): Cardesia parnassifolia, Nechamandra alternifolia, Potamogeton maackianus and P. octandrus. J. Jap. Bot. 84: 321–329.

Ito, Y., Nr. Tanaka, R. Pooma & Nb. Tanaka. 2014. DNA barcoding reveals a new record of Potamogeton dis-tinctus (Potamogetonaceae) and its natural hybrids, P. distinctus × P. nodosus and P. distinctus × P. wrightii (P. ×malainoides) from Myanmar. Biodiv. Data J. 2: e1073.

Kadono, Y. 1994. Aquatic Plants of Japan. Bun’ichisogo- Shuppan, Tokyo (in Japanese).

Kress, W. J., R. A. De Filipps, E. Farr & D. Y. Y. Kyi. 2003. A checklist of the Trees, Shrubs, Herbs, and Climbers of Union of Myanmar. Contributions from the Unites States National Herbarium 45: 1–590. Na-tional Museum of Natural History, Washington D.C.

Leach, G. J. & P. L. Osborne. 1985. Freshwater Plants of Papua New Guinea. University of Papua New Guinea Press, Port Moresby.

Little, D. P. & D. S. Barrington. 2003. Major evolutionary events in the origin and diversification of the fern ge-nus Polystichum (Dryopteridaceae). Amer. J. Bot. 90: 508–514.

Murata, J., T. Ohi-Toma & Nb. Tanaka. 2010. New or noteworthy plant collections from Myanmar (4): Ty-phonium cordifolium and two new species, T. neogracile and T. praecox (Araceae). J. Jap. Bot. 85: 1–7.

Ooi, K., Y. Endo, J. Yokoyama & N. Murakami. 1995. Useful primer designs to amplify DNA fragments of the plastid gene matK from angiosperm plants. J. Jap. Bot. 70: 328–331.

Pryer, K.M., E. Schuettpelz, P.G. Wolf, H. Schneider, A.R. Smith & R. Cranfill. 2004. Phylogeny and evo-lution of ferns (monilophytes) with a focus on the ear-ly leptosporangiate divergences. Amer. J. Bot. 91: 1582-1598.

Rendle, A.B. 1899. A systematic revision of the genus Na-jas. Trans. Linn. Soc. London, Bot. 5: 379–444.

Tanaka Nb. 2005. Plant inventory research: contributions to the flora of Myanmar. Acta Phytotax. Geobot. 56: 1–26.

long pits (Cook 1996). Cook (1996) includes SW China in the distribution range of N. tenuis, but the Flora of China excludes it (Wang et al. 2010).

Thanks are due to U Htun Paw Oo, Ex-director of Nature and Wildlife Conservation Division; U Soe Win Hlaing, Ex-director General, Forest Department, Ministry of En-vironmental Conservation and Forestry for their help in arranging our field work. We also thank Prof. Tetsuo Koyama, director of the Kochi Prefectural Makino Bo-tanical Garden (Japan), who initiated the Myanmar-Japa-nese cooperative program to inventory the plants of Myanmar, and gave us the opportunity to study the plants of Myanmar, D. Potter, University of California, Davis (USA), for reviewing a draft of the manuscript, and M. Kuroda of the University of Tokyo (Japan) for scanning voucher specimens. This research was partly supported by a Grant-in-Aid Aid (KAKENHI) for foreign expedi-tions (17255004 to Jin Murata) from the Japan Society for the Promotion of Science (JSPS), and a Grant-in-Aid from the Kochi Prefectural Government.

References

Altschul, S. F., W. Gish, W. Miller, E.W. Myers & D. J. Lipman. 1990. Basic local alignment search tool. J. Molec. Biol. 215: 403–410.

Altschul, S. F., T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller & D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 17: 3389–3402.

Campbell D. G. & H. D. Hammond H. D. 1989. Floristic Inventory of Tropical Countries: The status of plant systematics, collections, and vegetation, plus recom-mendations for the future. New York Botanical Gar-den, New York.

CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proc. Natl. Acad. Sci. USA. 106: 12794–12797.

Choi, H. -K. 2000. Aquatic vascular plants. Plant of Ko-rea 5: 1–220. Korea Research Institute of Bioscience and Biotechnology, Junghengsa, Seoul.

Cook, C. D. K. 1996. Aquatic and Wetland Plants of In-dia: A reference book and identification manual for the vascular plants found in permanent or seasonal fresh water in the subcontinent of India south of the Himalayas. Oxford University Press, Oxford.

Cook, C. D. K. & R. Lüönd. 1983. A revision of the genus Blyxa (Hydrocharitaceae). Aquatic Bot. 15: 1–52.

Dandy J. E. 1978. Hydrocharitaceae. In: Hara, H., W. T. Stearn & H. J. Williams (eds.), An Enumeration of the Flowering Plants of Nepal 1, pp. 29–30. Trustees of British Museum (Natural History), London.

Davenport, L. J. 1980. In: Chromosome number reports





long pits (Cook 1996), Cook (1996) includes SWChina in the distribution range ofN tenuis, butthe Flora of China excludes it (Wang et al, 2010),

Thanks are due to U Htun Paw Oo. Ex-director ofNature

and Wildlife Conservation Division; U Soe Win Hlaing.

Ex-director General, Forest Department. Ministry ofEn-

vironmental Conservation and Forestry tbr their help inarranging our field work. We also thank Protl Tbtsuo

Koyama, director of the Kochi Prefectural Makino Bo-tanical Garden (Japan). who initiated the MyanmaFJapa-nese cooperative program to inventory the plants of

Myanmar, and gave us the opportunity to study the plantsof Myanmar. D. Potter. University of Calitbrnia. Davis

(USA). for reviewing a draft of the manuscript, and M.

Kuroda of the University of Tbkyo (Japan) for scanningvoucher specimens. This research was partly supported

by a Grant-in-Aid Aid (KAKENHI) for tbreign expedi-tions (17255004 to Jin Murata) from the Japan Society forthe Promotion ofScience (JSPS), and a Grant-in-Aid fromthe Kochi Prefectural Government.

References

Altschul. S. F., W. Gish, W. Miller. E.W. Myers & D. J.

Lipman. 1990. Basic local alignment search tool. J. Molec. Biol. 215: 403-410.

Altschul. S. F.. T. L. Madden. A. A. Schliffer, J. Zhang. Z.

Zhang, W. Miller & D. J. Lipman. 1997. Gapped

BLAST and PSI-BLAST: a new generation ofprotein

database search programs. Nucleic Acids Res. 17: 3389-3402.

Campbell D. G. & H. D. Hammond H. D. 1989. Floristic

Inventory of Tropical Countries: The status of plant

systematics. collections. and vegetation, plus recom- mendations tbr the future. New Ybrk Botanical Gar-

den, New York.

CBOL PIant Working Group. 2009. A DNA barcode for

land plants. Proc. Natl. Acad. Sci. USA. 106: 12794-

12797.

Choi, H. -K. 2000. Aquatic vascular plants. Plant of Ko- rea 5: 1-220. Korea Research Institute ofBioscience

and Biotechnology. Junghengsa. Seoul.

Cook, C. D. K. 1996. Aquatic and Wetland Plants of In- dia: A reference book and identification manual for

the vascular plants tbund in permanent or seasonal

fresh water in the subcontinent of India south of the

Himalayas. Oxford University Press. Oxford.

Cook, C. D. K. & R. LU6nd. 1983. A revision ofthe genus

Blyxa (Hydrocharitaceae). Aquatic Bot. 15: 1-52.

Dandy J. E. 1978. Hydrocharitaceae. in: Hara, H., W. T.

Stearn&H.J.Wi11iams(eds.),AnEnumerationofthe

Flowering Plants ofNepal 1. pp. 29-30. ftustees of

British Museum (Natural History), London.Davenport. L. J. 1980. In: Chromosome number reports

LXVII. Thxon 29: 351.den Hartog C. 1957. Hydrocharitaceae. ih: van Steenis. C.

G. G. J. (ed.). Flora Malesiana sen 1. 5: 381-413.

Noordhofg Leyden.Hayens, R. R. 2001. Hydrocharitaceae. in: Santisuk, T.

and K. Larsen (eds.), Flora ofThailand 7. pp. 365-

382. The Forest Herbarium, Royal Forest Depart-

ment.Bangkok.

Ito. Y.. T. Ohi:Tbma, J. Murata & Nr. fanaka. 2010. Hy-

bridization and polyploidy of an aquatic plant. Rz{p-

pia (Ruppiaceae). inferred from plastid and nuclear

DNA phylogenies. Amer. J. Bot. 97: 1156-1167.

Ito. Y.. Nb. Tanaka. T. Ohi:Tbma & J. Murata. 2009. New

or noteworthy plant collection from Myanmar (3): CZirdesia parnassijbtia. Nbchamandra alternijblia.

Potamogeton maackianus and P. octandrus. J. Jap. Bot. 84: 321-329.

Ito. Y.. Nn Tanaka. R. Pooma & Nb. fanaka. 2014. DNA

barcoding reveals a new record of Potamogeton dis-

tinctus (Potamogetonaceae) and its natural hybrids,

P. distinctus x P. nodosus and P. distinctus x P.

ivrightii (P. xmalainoides) from Myanmar. Biodiv.

Data J. 2: el073.

Kadono. Y. 1994. Aquatic Plants ofJapan. Bun'ichisogo-

Shuppan, Tokyo (in Japanese).Kress, W. J., R. A. De Filipps, E. Farr & D. Y. Y. Kyi.

2003. A checklist of the Trees. Shrubs, Herbs, and

Climbers of Union of Myanmar. Contributions from the Unites States National Herbarium 45: 1-590. Na-

tional Museum ofNatural History, Washington D.C.

Leach. G. J. & P. L. Osborrie. 1985. Freshwater Plants of

PapuaNewGuinea.UniversityofPapuaNewGuinea

Press,PortMoresby.

Little. D. P. & D. S. Barrington. 2003. Major evolutionary

events in the origin and diversification ofthe fern ge-

nus Polystichum (Dryopteridaceae). Amer. J. Bot. 90:

508-514.Murata, J.. T. Ohi:Tbma & Nb. fanaka. 2010. New or

noteworthy plant collections from Myanmar O): OL phonium cordijbtium and two new species, T

neogracile and T praecox (Araceae). J. Jap. Bot. 85:

1-7.Ooi, K.. Y. Endo. J. Ybkoyama & N Murakami. 1995.

Usefu1 primer designs to amplify DNA fragments of

the plastid gene mati( from angiospenn plants. J. Jap. Bot 70: 328-331.Pryer. K.M., E. Schuettpelz, P.G. Wolg H. Schneider,

A.R. Smith & R. Cranfill. 2004. Phylogeny and evo-

lution of ferns (monilophytes) with a tbcus on the ear-

ly leptosporangiate divergences. Amen J. Bot. 91: 1582-1598.

Rendle,A.B.1899.AsystematicrevisionofthegenusNd-

jas. Trans. Linn. Soc. London, Bot. 5: 379-444.fanaka Nb. 2005. Plant inventory research: contributions

to the fiora of Myanmar. Acta Phytotax. Geobot. 56: 1-26.



long pits (Cook 1996), Cook (1996) includes SWChina in the distribution range ofN tenuis, butthe Flora of China excludes it (Wang et al, 2010),

Thanks are due to U Htun Paw Oo. Ex-director ofNature

and Wildlife Conservation Division; U Soe Win Hlaing.

Ex-director General, Forest Department. Ministry ofEn-

vironmental Conservation and Forestry tbr their help inarranging our field work. We also thank Protl Tbtsuo

Koyama, director of the Kochi Prefectural Makino Bo-tanical Garden (Japan). who initiated the MyanmaFJapa-nese cooperative program to inventory the plants of

Myanmar, and gave us the opportunity to study the plantsof Myanmar. D. Potter. University of Calitbrnia. Davis

(USA). for reviewing a draft of the manuscript, and M.

Kuroda of the University of Tbkyo (Japan) for scanningvoucher specimens. This research was partly supported

by a Grant-in-Aid Aid (KAKENHI) for tbreign expedi-tions (17255004 to Jin Murata) from the Japan Society forthe Promotion ofScience (JSPS), and a Grant-in-Aid fromthe Kochi Prefectural Government.

References

Altschul. S. F., W. Gish, W. Miller. E.W. Myers & D. J.

Lipman. 1990. Basic local alignment search tool. J. Molec. Biol. 215: 403-410.

Altschul. S. F.. T. L. Madden. A. A. Schliffer, J. Zhang. Z.

Zhang, W. Miller & D. J. Lipman. 1997. Gapped

BLAST and PSI-BLAST: a new generation ofprotein

database search programs. Nucleic Acids Res. 17: 3389-3402.

Campbell D. G. & H. D. Hammond H. D. 1989. Floristic

Inventory of Tropical Countries: The status of plant

systematics. collections. and vegetation, plus recom- mendations tbr the future. New Ybrk Botanical Gar-

den, New York.

CBOL PIant Working Group. 2009. A DNA barcode for

land plants. Proc. Natl. Acad. Sci. USA. 106: 12794-

12797.

Choi, H. -K. 2000. Aquatic vascular plants. Plant of Ko- rea 5: 1-220. Korea Research Institute ofBioscience

and Biotechnology. Junghengsa. Seoul.

Cook, C. D. K. 1996. Aquatic and Wetland Plants of In- dia: A reference book and identification manual for

the vascular plants tbund in permanent or seasonal

fresh water in the subcontinent of India south of the

Himalayas. Oxford University Press. Oxford.

Cook, C. D. K. & R. LU6nd. 1983. A revision ofthe genus

Blyxa (Hydrocharitaceae). Aquatic Bot. 15: 1-52.

Dandy J. E. 1978. Hydrocharitaceae. in: Hara, H., W. T.

Stearn&H.J.Wi11iams(eds.),AnEnumerationofthe

Flowering Plants ofNepal 1. pp. 29-30. ftustees of

British Museum (Natural History), London.Davenport. L. J. 1980. In: Chromosome number reports

LXVII. Thxon 29: 351.den Hartog C. 1957. Hydrocharitaceae. ih: van Steenis. C.

G. G. J. (ed.). Flora Malesiana sen 1. 5: 381-413.

Noordhofg Leyden.Hayens, R. R. 2001. Hydrocharitaceae. in: Santisuk, T.

and K. Larsen (eds.), Flora ofThailand 7. pp. 365-

382. The Forest Herbarium, Royal Forest Depart-

ment.Bangkok.

Ito. Y.. T. Ohi:Tbma, J. Murata & Nr. fanaka. 2010. Hy-

bridization and polyploidy of an aquatic plant. Rz{p-

pia (Ruppiaceae). inferred from plastid and nuclear

DNA phylogenies. Amer. J. Bot. 97: 1156-1167.

Ito. Y.. Nb. Tanaka. T. Ohi:Tbma & J. Murata. 2009. New

or noteworthy plant collection from Myanmar (3): CZirdesia parnassijbtia. Nbchamandra alternijblia.

Potamogeton maackianus and P. octandrus. J. Jap. Bot. 84: 321-329.

Ito. Y.. Nn Tanaka. R. Pooma & Nb. fanaka. 2014. DNA

barcoding reveals a new record of Potamogeton dis-

tinctus (Potamogetonaceae) and its natural hybrids,

P. distinctus x P. nodosus and P. distinctus x P.

ivrightii (P. xmalainoides) from Myanmar. Biodiv.

Data J. 2: el073.

Kadono. Y. 1994. Aquatic Plants ofJapan. Bun'ichisogo-

Shuppan, Tokyo (in Japanese).Kress, W. J., R. A. De Filipps, E. Farr & D. Y. Y. Kyi.

2003. A checklist of the Trees. Shrubs, Herbs, and

Climbers of Union of Myanmar. Contributions from the Unites States National Herbarium 45: 1-590. Na-

tional Museum ofNatural History, Washington D.C.

Leach. G. J. & P. L. Osborrie. 1985. Freshwater Plants of

PapuaNewGuinea.UniversityofPapuaNewGuinea

Press,PortMoresby.

Little. D. P. & D. S. Barrington. 2003. Major evolutionary

events in the origin and diversification ofthe fern ge-

nus Polystichum (Dryopteridaceae). Amer. J. Bot. 90:

508-514.Murata, J.. T. Ohi:Tbma & Nb. fanaka. 2010. New or

noteworthy plant collections from Myanmar O): OL phonium cordijbtium and two new species, T

neogracile and T praecox (Araceae). J. Jap. Bot. 85:

1-7.Ooi, K.. Y. Endo. J. Ybkoyama & N Murakami. 1995.

Usefu1 primer designs to amplify DNA fragments of

the plastid gene mati( from angiospenn plants. J. Jap. Bot 70: 328-331.Pryer. K.M., E. Schuettpelz, P.G. Wolg H. Schneider,

A.R. Smith & R. Cranfill. 2004. Phylogeny and evo-

lution of ferns (monilophytes) with a tbcus on the ear-

ly leptosporangiate divergences. Amen J. Bot. 91: 1582-1598.

Rendle,A.B.1899.AsystematicrevisionofthegenusNd-

jas. Trans. Linn. Soc. London, Bot. 5: 379-444.fanaka Nb. 2005. Plant inventory research: contributions

to the fiora of Myanmar. Acta Phytotax. Geobot. 56: 1-26.


Tanaka, Nb. 2012a. Studies of Zingiberaceae in Myanmar I: Zingiber idae, newly recorded from Myanmar. Makinoa New Series 10: 153–157.

Tanaka, Nb. 2012b. Studies of Zingiberaceae in Myanmar II: Zingiber popaense, a new species from Myanmar. Makinoa New Series 10: 159–163.

Tanaka, Nb. & Y. Hayami. 2011. Begonia kachinensis (Begoniaceae, sect. Sphenanthera), a New Species from Myanmar. Acta Phytotax. Geobot. 61: 151–154.

Tanaka, Nb & M. Hughes. 2007. Begonia (sect. Sphenan-thera) hayamiana (Begoniaceae), a new species from northern Myanmar. Acta Phytotax. Geobot. 58: 83–86.

Tanaka, Nb. & H. Nagamasu. 2006. A new record of Gmelina tomentosa Fletcher (Verbenaceae) from Myanmar. Acta Phytotax. Geobot. 57: 233–236.

Tanaka, Nb., Y. Kadota & J. Murata. 2010c. New or note-worthy plant collection from Myanmar (6): Ranuncu-laceae of Mt. Victoria. J. Jap. Bot. 85: 199–212.

Tanaka, Nb., S. Kobayashi, T. Ohi-Toma & J. Murata. 2006a. New or noteworthy plant collections from Myanmar (1) Hydrobryum japonicum, Balanophora subcupularis, Rhopalocnemis phalloides and Soneri-la laeta. J. Jap. Bot. 81: 324–331.

Tanaka, Nb., T. Koyama & J. Murata. 2006b. The flower-ing plants of Mt. Popa, Central Myanmar – results of Myanmar-Japanese Joint Expeditions 2000–2004. Makinoa New Series 5: 1–95.

Tanaka, Nb., T. Ohi-Toma & J. Murata. 2009. A taxonom-ic study of Rosa clinophylla var. glabra (Rosaceae), newly recorded from Myanmar, based on morpholog-ical and molecular data. J. Jap. Bot. 84: 27–32.

Tanaka, Nb., T. Ohi-Toma & J. Murata. 2010b. A new spe-cies of Argostemma (Rubiaceae) from Mt. Victoria, Myanmar. Blumea 55: 65–67.

Tanaka, Nb., Nr. Tanaka, T. Ohi-Toma & J. Murata. 2007. New or noteworthy plant collections from Myanmar (2) Aponogeton lakhonensis, Cryptocoryne crudda-siana, C. crispatula var. balansae and Stichoneuron membranaceum. J. Jap. Bot. 82: 266–273.

Tanaka, Nb., T. Yukawa & J. Murata. 2010a. New or note-

worthy plant collection from Myanmar (5): Dendro-bium koyamae, a new species in sect. Formosa (Or-chidaceae). Acta Phytotax. Geobot. 60: 171–174.

Tanaka, Nb., T. Yukawa, K. M. Htwe, T. Koyama & J. Murata. 2011. New or noteworthy plant collections from Myanmar (7): Fourteen additional species of Orchidaceae. Acta Phytotax. Geobot. 61: 161–165.

van Dijk, P. P., A. W. Tordoff, J. Fellowes, M. Lau & M. Jinshuang. 2004. Indo-Burma. In: Mittermeier, R. A., G. P. Robles, M. Hoffmann, J. Lamoreaux & G. A. B. da Fonseca (eds.), Hotspots Revisited: Earth’s Biolog-ically Richest and Most Endangered Terrestrial Ecoregions, pp. 323–330. CEMEX, Agrupación Si-erra Madre.

Wang, H. & X. Sun. 1992. Hydrocharitaceae. In: Sun, X., H.Wang, Q. Li, Y. Guo, L. Zhou, J. You, X. Zhong & Y. Chen (eds), Flora Republicae Popularis Sinicae 8: 151–190. Science Press, Beijing (in Chinese).

Wang, Q. F., G. Youhao, R. R. Haynes & C. B. Hellquist. 2010. Hydrocharitaceae. In: Wu, Z. & P. H. Raven (eds.), Flora of China. 23, pp. 91–102. Science Press, Beijing & Missouri Botanical Garden Press, St. Lou-is.

Whitten, W. M., N. H. Williams, & M. W. Chase. 2000. Subtribal and generic relationships of Maxillarieae (Orchidaceae) with emphasis on Stanhopeinae: com-bined molecular evidence. Amer. J. Bot. 87: 1842–1856.

Wolf, P. G., P. S. Soltis & D. E. Soltis. 1994. Phylogenetic relationships of dennstaedtioid ferns: Evidence from rbcL. Molec. Phylogen. Evol. 3: 383–392.

You, J. 1989. IOPB chromosome data 1. Int. Organ. Pl. Biosyst. Newslett. (Zurich) 13: 21.

You, J., X. Z. Sun & H. Q. Wang. 1985. Taxonomy of Na-jas: A synthetical analysis with evidences on cytolo-gy, isozymes and SEM examination. Wuhan Daxue Ziran Kexue Xuebao (J. Wuhan Univ., Nat. Sci. Ed.). 4: 111–118 (in Chinese).

Yukawa, T., Nb. Tanaka & J. Murata. 2010. Doritis nat-mataungensis (Orchidaceae), a new species from Myanmar. Acta Phytotax. Geobot. 60: 167–170.

Received March 09, 2013; accepted November 18, 2013




June2014 Ito&AL.-NeworNoteworthyPlantCollectionsfromMyanmar(8) 97

fanaka.Nb.2012a.StudiesofZingiberaceaeinMyanmar

I: Zingiber idae. newly recorded from Myanmar. MakinoaNew Series 10: 153-157.fanaka.Nb.2012b.StudiesofZingiberaceaeinMyanmar

II: Z'ngiberpqpaense, a new species from Myanmar. MakinoaNew Series 10: 159-163.Thnaka. Nb. & Y. Hayami. 2011. Begonia kachinensis

(Begoniaceae, sect. Sphenanthera), a New Species

from Myanmar. Acta Phytotax. Geobot. 61: 151-154.

Thnaka. Nb & M. Hughes. 2007. Begonia (sect. sphenanthera) hayamiana (Begoniaceae). a new species from

northern Myanmar. Acta Phytotax. Geobot. 58: 83-

86.Thnaka. Nb. & H. Nagamasu. 2006. A new record of

Gmetina tomentosa Fletcher (Verbenaceae) from

Myanmar. Acta Phytotax. Geobot. 57: 233-236.fanaka. Nb.. Y. Kadota & J. Murata. 2010c. New or note-

worthy plant collection from Myanmar (6): Ranuncu-

laceae ofMt. Victoria. J. Jap. Bot. 85: 199-212.fanaka. Nb., S. Kobayashi. Z Ohi:Tbma & J. Murata.

2006a. New or noteworthy plant collections from

Myanmar (1) llydrobryum japonicum. Balanqphora

subcuputaris,RhopatocnemisphattoidesandSoneri-

ta taeta. J. Jap. Bot. 81: 324-331.fanaka. Nb.. T. Koyama & J. Murata. 2006b. The fioweF

ing plants of Mt. Popa. Central Myanmar - results of

Myanmar-Japanese Joint Expeditions 2000-2004.

MakinoaNew Series 5: 1-95.fanaka. Nb., T. Ohi:Tbma & J. Murata. 2009. A taxonom-

ic study ofRosa ctinqpitytta var. gtabra (Rosaceae), newlyrecordedfromMyanmar,basedonmorpholog-

ical and molecular data. J. Jap. Bot. 84: 27-32.fanaka. Nb.. T. Ohi:Tbma & J. Murata. 2010b. A new spe-

cies ofArgostemma (Rubiaceae) from Mt. Victoria,

Myanmar. Blumea 55: 65-67.Thnaka. Nb., Nr. Tanaka, T. Ohi:Tbma & J. Murata. 2007.

New or noteworthy plant collections from Myanmar

(2) Aponogeton lakhonensis. Cmptocoryne crudda-

siana. C crispatula var. balansae and Stichoneuron

membranaceum. J. Jap. Bot. 82: 266-273.fanaka. Nb.. T. Yukawa & J. Murata. 201Oa. New or note-

worthy plant collection from Myanmar (5): Dendro-

bium kayamae, a new species in sect. Fbrmosa (Or- chidaceae). Acta Phytotax. Geobot. 60: 171-174.Tanaka, Nb., T. Yukawa, K. M. Htwe. T. Koyama & J. Murata. 2011. New or noteworthy plant collections

from Myanmar (7): Fourteen additional species of

Orchidaceae. Acta Phytotax. Geobot. 61: 161-165.van Dljk, R P.. A. W. TbrdofiL J. Fellowes. M. Lau & M.

Jinshuang. 2004. Indo-Burma. in: Mittermeier. R. A..

G. P. Robles. M. Hoennann, J. Lamoreaux & G. A. B.

daFonseca(eds.).HotspotsRevisited:Earth'sBiolog-

ically Richest and Most Endangered Tlerrestrial

Ecoregions. pp. 323-330. CEMEX. Agrupaci6n Si- erra Madre.

Wang. H. & X. Sun. 1992. Hydrocharitaceae. ih: Sun, X..

H.Wang. Q. Li. Y. Guo. L. Zhou, J. Ybu, X. Zhong &

Y. Chen (eds), Flora Republicae Popularis Sinicae 8:

151-190. Science Press. Beij'ing (in Chinese).Wang. Q. F., G. Youhao. R. R. Haynes & C. B. Hellquist.

2010. Hydrocharitaceae. ih: Wu, Z. & P. H. Raven

(eds.), Flora of China. 23. pp. 91-102. Science Press.

Beijing & Missouri Botanical Garden Press, St. Lou- is.Whitten,

SM M., N. H. Williams, & M. W. Chase. 2000.

Subtribal and generic relationships of Maxillarieae

(Orchidaceae) with emphasis on Stanhopeinae: com-

bined molecular evidence. Amer. J. Bot. 87: 1842-

1856.Wolg

P. G., P. S. Soltis & D. E. Soltis. 1994. Phylogenetic

relationships of dennstaedtioid ferns: Evidence from

rbcL. Molec. Phylogen. Evol. 3: 383-392.You. J. 1989. IOPB chromosome data 1. Int. Organ. Pl.

Biosyst. Newslett. (Zurich) 13: 21.

You. J.. X. Z. Sun & H. Q. Wang. 1985. Thxonomy ofNlr-

jas: A synthetical analysis with evidences on cytolo-

gy, isozymes and SEM examination. Wuhan Daxue

Ziran Kexue Xuebao (J. Wuhan Univ.. Nat. Sci. Ed.).

4: 111-118 (in Chinese).Yukawa. T.. Nb. fanaka & J. Murata. 2010. Doritis nat-

mataungensis (Orchidaceae), a new species from

Myanmar. Acta Phytotax. Geobot. 60: 167-170.

Receii,ed Mlrrch 09, 2013; accepted Nbi,ember 18, 2013

NII-Electronic


June2014 Ito&AL.-NeworNoteworthyPlantCollectionsfromMyanmar(8) 97

fanaka.Nb.2012a.StudiesofZingiberaceaeinMyanmar

I: Zingiber idae. newly recorded from Myanmar. MakinoaNew Series 10: 153-157.fanaka.Nb.2012b.StudiesofZingiberaceaeinMyanmar

II: Z'ngiberpqpaense, a new species from Myanmar. MakinoaNew Series 10: 159-163.Thnaka. Nb. & Y. Hayami. 2011. Begonia kachinensis

(Begoniaceae, sect. Sphenanthera), a New Species

from Myanmar. Acta Phytotax. Geobot. 61: 151-154.

Thnaka. Nb & M. Hughes. 2007. Begonia (sect. sphenanthera) hayamiana (Begoniaceae). a new species from

northern Myanmar. Acta Phytotax. Geobot. 58: 83-

86.Thnaka. Nb. & H. Nagamasu. 2006. A new record of

Gmetina tomentosa Fletcher (Verbenaceae) from

Myanmar. Acta Phytotax. Geobot. 57: 233-236.fanaka. Nb.. Y. Kadota & J. Murata. 2010c. New or note-

worthy plant collection from Myanmar (6): Ranuncu-

laceae ofMt. Victoria. J. Jap. Bot. 85: 199-212.fanaka. Nb., S. Kobayashi. Z Ohi:Tbma & J. Murata.

2006a. New or noteworthy plant collections from

Myanmar (1) llydrobryum japonicum. Balanqphora

subcuputaris,RhopatocnemisphattoidesandSoneri-

ta taeta. J. Jap. Bot. 81: 324-331.fanaka. Nb.. T. Koyama & J. Murata. 2006b. The fioweF

ing plants of Mt. Popa. Central Myanmar - results of

Myanmar-Japanese Joint Expeditions 2000-2004.

MakinoaNew Series 5: 1-95.fanaka. Nb., T. Ohi:Tbma & J. Murata. 2009. A taxonom-

ic study ofRosa ctinqpitytta var. gtabra (Rosaceae), newlyrecordedfromMyanmar,basedonmorpholog-

ical and molecular data. J. Jap. Bot. 84: 27-32.fanaka. Nb.. T. Ohi:Tbma & J. Murata. 2010b. A new spe-

cies ofArgostemma (Rubiaceae) from Mt. Victoria,

Myanmar. Blumea 55: 65-67.Thnaka. Nb., Nr. Tanaka, T. Ohi:Tbma & J. Murata. 2007.

New or noteworthy plant collections from Myanmar

(2) Aponogeton lakhonensis. Cmptocoryne crudda-

siana. C crispatula var. balansae and Stichoneuron

membranaceum. J. Jap. Bot. 82: 266-273.fanaka. Nb.. T. Yukawa & J. Murata. 201Oa. New or note-

worthy plant collection from Myanmar (5): Dendro-

bium kayamae, a new species in sect. Fbrmosa (Or- chidaceae). Acta Phytotax. Geobot. 60: 171-174.Tanaka, Nb., T. Yukawa, K. M. Htwe. T. Koyama & J. Murata. 2011. New or noteworthy plant collections

from Myanmar (7): Fourteen additional species of

Orchidaceae. Acta Phytotax. Geobot. 61: 161-165.van Dljk, R P.. A. W. TbrdofiL J. Fellowes. M. Lau & M.

Jinshuang. 2004. Indo-Burma. in: Mittermeier. R. A..

G. P. Robles. M. Hoennann, J. Lamoreaux & G. A. B.

daFonseca(eds.).HotspotsRevisited:Earth'sBiolog-

ically Richest and Most Endangered Tlerrestrial

Ecoregions. pp. 323-330. CEMEX. Agrupaci6n Si- erra Madre.

Wang. H. & X. Sun. 1992. Hydrocharitaceae. ih: Sun, X..

H.Wang. Q. Li. Y. Guo. L. Zhou, J. Ybu, X. Zhong &

Y. Chen (eds), Flora Republicae Popularis Sinicae 8:

151-190. Science Press. Beij'ing (in Chinese).Wang. Q. F., G. Youhao. R. R. Haynes & C. B. Hellquist.

2010. Hydrocharitaceae. ih: Wu, Z. & P. H. Raven

(eds.), Flora of China. 23. pp. 91-102. Science Press.

Beijing & Missouri Botanical Garden Press, St. Lou- is.Whitten,

SM M., N. H. Williams, & M. W. Chase. 2000.

Subtribal and generic relationships of Maxillarieae

(Orchidaceae) with emphasis on Stanhopeinae: com-

bined molecular evidence. Amer. J. Bot. 87: 1842-

1856.Wolg

P. G., P. S. Soltis & D. E. Soltis. 1994. Phylogenetic

relationships of dennstaedtioid ferns: Evidence from

rbcL. Molec. Phylogen. Evol. 3: 383-392.You. J. 1989. IOPB chromosome data 1. Int. Organ. Pl.

Biosyst. Newslett. (Zurich) 13: 21.

You. J.. X. Z. Sun & H. Q. Wang. 1985. Thxonomy ofNlr-

jas: A synthetical analysis with evidences on cytolo-

gy, isozymes and SEM examination. Wuhan Daxue

Ziran Kexue Xuebao (J. Wuhan Univ.. Nat. Sci. Ed.).

4: 111-118 (in Chinese).Yukawa. T.. Nb. fanaka & J. Murata. 2010. Doritis nat-

mataungensis (Orchidaceae), a new species from

Myanmar. Acta Phytotax. Geobot. 60: 167-170.

Receii,ed Mlrrch 09, 2013; accepted Nbi,ember 18, 2013

NII-Electronic

(Araceae), (Hydrocharitaceae) (Araceae), (Hydrocharitaceae)

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