Tema 15 Fisio
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Transcript of Tema 15 Fisio
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Ciclo celular de procariotas I
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Ciclo
celular de procariotas II
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La tcnica baby-machine
Exponentially growing culture lB
I C 101Period
Elutiontime O0.25 0.5 0.75 1Cellage
Fiq. 22.2a-c Th~membrane-elution tech-nique. .a An exponentially growingpopulation of
Membrane
(generations
O 0000
!
Mernbranesurface
bacteria is rapidly c"#lled andt"en passedt"roug" a nitrocellulosefilter$ %"en asufficient number of cells
"a&e been collected on t"e filter$ t"efilter de&ice is
upport0.25
00000!roplets
in&erted$ and prewarmedfres" culture me diumispassedt"roug" t"e filter$'"e bacteria remainattac"ed to t"e filter andgrow on its
ofwaste
0.5
medium
00
0
00
surface$ %"en a celldi&ides$ one of t"e
daug"ter cells (newborn cellcan be eluted
..
0.75
In&ert filter de&iceb
!Elutewit"
c(() 1.0fres" medium )(i*
and eit"er collected andc"illed as starting material$for a sync"ronous cultureor col lected o&er time infractions for analysis$b '"e exponentialpopulation consists of cellsof different ages$ .ewborn
cells are of age O. and cellst"at are finis"ingcell di&isionare of age 1. '"ese arerepresented on an enlarged
..
!
* !
..
0000
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imirnt
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!ecimiento de la
membana
% 5
!
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Crecimiento de la pared
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Crecimiento de la pared de cocos 7ram 8
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9l cluster de :enes de di"ision
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9l replisoma
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"e7aacin de lo* nucleoide* hi;o*
5a;a 9elocidad de cecimiento
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%lta 9elocidad de cecimiento
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"e8e8acin de lo* nucleoide*
&ote
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&otee en 7ocaiota*
%ctina =e5 &a=
!itoe*?ueleto
eucaitico
@oma celula
7ocaiota*
Re7ato %A
7ocaiota*
@ilamento* &a= enE. coli !able* =e5 en clula* bacilae*
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epto de di"isi;n
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FTs< = anillo
ecuatorial
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@t*K
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Reclutamiento de ota* 7ote
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%nillo K
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Ki7% y @t*%
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+ede* et al. DEEF. 5acteial mitotic machineie*. !ell GGH, J4-H
he ob*e9ation* de*cibed heein e9eal that,
*u7i*in8ly, Aatue ha* cho*en to u*e actin and
tubulin fo eci7ocal 7u7o*e* in t/o diffeent
domain* of life: In mo*t eu>ayotic cell ty7e*, actini* a fundamental com7onent of the cyto>inetic in8
/hile tubulin fom* the mitotic *7indle fibe*. In
conta*t, in bacteia, the ubi?uitou* tubulin-li>e
@t*K 7otein fom* the cyto>inetic in8, /hile the
actin-li>e 7otein &a= (and 7o**ibly =e5M fom*
a mitotic-li>e machiney that *e8e8ate* A%.
Locali6acin ecuatoial del *e7to
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Locali6acin ecuatoial del *e7to
il i d l
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*cilacin del
*i*tema =in!B
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=in!
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=in
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eteminacin de la 7o*icin
del *e7to
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! i li i di i i
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Tiempo de
:eneraci;n0minutos-
>0
50
?0
&0
!oneNin ente e7licacin y di9i*in
Ciclo celular 0minutos-
0 10 20 &0 ?0 50 >0
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Bfecto de di9e*a* mutacione* (E. coliM
dnaA dnaE
minC,D,E gyrB
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envA ftsI,Q,Z
ftsA