praktikum tropis

8/10/2019 praktikum tropis

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Dr. Ryzqa

BAGIAN MIKROBIOLOGI

FK UNHAS

PRAKTIKUM TROPIS

“Bacalah dengan (menyebut)

nama Rabbmu Yangmenciptakan, (QS. 96:1)



OSTE08LAST READY???



YANG AKAN DIPRAKTIKUMKAN1. Me!"a# $a%&ara' (!)a# &!*+!%!a, -a' %e-!%

#ra'(/*r -a' %e-!% !(*a(! -ar! Salmonellathypi

. Me!"a# $a%&ara' %!+r*(+*/!(

Mycobacterium leprae. Me!"a# Ga%&ara' %!+r*(+*/!( -ar!

Corynebacterium diphtheriae

2. Me!"a# $a%&ara' %!+r*+*/!( -a' +**'! -ar!

Microsporum, Epidermophyton dan,Trichophyton

3. Me!"a# $a%&ara' %!+r*(+*/!( -a' $a%&ara'+**'! -ar! Pytorosporum orbiculare, Piedra

hortae, -a' Trichosporum beigelii



PRAKTIKUM MIKROBIOLOGI TROPIS

BAKTERI

S. #"y/!! M. Le/rae6.

-!/"#er!ae

7AMUR

DERMATOFI TA

NONDERMATOFI

TA

Tr!5"*/"y#*', (/

M!5r*(/*r%, (/

E/!-er%*/"y#*' (/

P!#yr*(/*r%

*r&!5are

P!e-ra

"*r#ae

Tr!5"*(/*r

*' &e!$e!!

A(/er$!(

Pe'!5!!%



&a+#er!1. Sa%*'ea #"y/!!

. My5*&a5#er!% Le/rae

. 6*ry'*&a5#er!% -!/"#er!e



Sa%*'ea

K!'$-*%

Ba5#er!a

P"y% Pr*#e*&a5#er!a

6a(( Ga%%a/r*#e*&a5#er!a

Or-* E'#er*&a5#er!ae(

Fa%!y e'#er*&a5#er!a5!ae

Ge'( Sa%*'ea



Salmonella typhi a-aa" a'$$*#a $e'(Enterobacteriaceae, ya!# +e*%/*+ &a+#er!ya'$ &er(!)a# 'e$a#!) Gra%, &er&e'#+ &a(!ya'$ /e*%*r)> a-a ya'$ /e'-e+ a-a ya'$

/a'<a'$, %aa" +a-a'$ %e'yer/a! a%e'a%e',B!(a -!!(*a(! -ar! -ara" /a-a %!'$$ /er#a%a

/e'ya+!# a#a -ar! #!'<a /a-a (#a-!% a'<#/e'ya+!#,Pa-a +arr!er, Salmonella typhi &!(a #!'$$a

a%a -aa% +a'-'$ e%/e-, (e"!'$$a &!(a-!!(*a(! -ar! #!'<a -aa% @a+# ya'$ a%a.



Me-!% #ra'(/*r# '#+>

S/e5!%e' -ara" a-aa" %e-!%+a- e%/e- O B!eC,

S/e5!%e' #!'<a a-aa" %e-!%Ra//a/*r# a#a %e-!% (ee'!#.

%e-!% (ee+#!) '#+ !(*a(!Salmonella typhi a-aa" %e-!%Sa%*'ea S"!$ea SSC a$ar.



Selenit medium Rappaport medium



Sa%*'ea *'

SS a$ar>6**re(( @!#"

&a5+5e'#er.



My5*&a5#er!% e/rae

K!'$-*%> Ba5#er!a

P"y%> A5#!'*&a5#er!a

Or-er> A5#!'*%y5e#ae(

S&*r-er> 6*ry'e&a5#er!'eae

Fa%!y> My5*&a5#er!a5eae

Ge'(> My5*&a5#er!%

S/e5!e(> M. e/rae

MB e/r*(y

T&er5*!- e/r*(yLe/r*%a#*(

http://en.wikipedia.org/wiki/Bacteria

http://en.wikipedia.org/wiki/Actinobacteria

http://en.wikipedia.org/wiki/Actinomycetales


http://en.wikipedia.org/wiki/Corynebacterineae


http://en.wikipedia.org/wiki/Mycobacterium












http://en.wikipedia.org/wiki/Actinobacteria

http://en.wikipedia.org/wiki/Bacteria



My5*&a5#er!% e/raeBer&e'#+ &a(! -e'$a' +ra' /a'<a'$

9% -a' e&ar 0.0.2 %

Pe*%*r/"!5

*&!$a#e !'#ra5ear

Ber(!)a# #a"a' a(a% -a' $ra% /*(!#!)

Pe'ye&a& /e'ya+!# e/ra

Me%!!+! 3 a/!(a' %e%&ra' ar a#a

e'e*/e



Ba"a' /e%er!+(aa' ya'$ -!/a+a! '#+/e%er!+(aa' a-aa" 5a!ra' +!# -ar! -e)e+ya'$ -!5r!$a! e/ra, ya'$ +e%-!a' -!&a#/ra/ara# "a/( (+!' (%earC,

Ba+#er! !'! &er&e'#+ &a#a'$ ya'$ &er(!)a##a"a' a(a% BTAC ya'$ &!a(a'ya&er+e*%/*+ -aa% &e'#+ $*&!,6ara /e@ar'aa' ya'$ &!a(a -!/a+a! '#+

%e!"a# $a%&ara' Mycobacterium leprae a-aa" /e@ar'aa' !e"Nee(e' (a#/e@ar'aa' #a"a' a(a%C



6*ry'e&a5#er!% -!/"#"er!ae

Kera<aa'> Ba5#er!a

F!%> A5#!'*&a5#er!a

Or-*> A5#!'*%y5e#ae(

Fa%!!> 6*ry'e&a5#er!a5eae

Ge'(> Corynebacterium

S/e(!e(> !. diphthe"iae

http://id.wikipedia.org/wiki/Bacteria

http://id.wikipedia.org/wiki/Actinobacteria

http://id.wikipedia.org/w/index.php?title=Actinomycetales&action=edit&redlink=1

http://id.wikipedia.org/w/index.php?title=Corynebacteriaceae&action=edit&redlink=1


http://id.wikipedia.org/w/index.php?title=Corynebacterium&action=edit&redlink=1

http://id.wikipedia.org/w/index.php?title=Corynebacterium&action=edit&redlink=1



http://id.wikipedia.org/w/index.php?title=Actinomycetales&action=edit&redlink=1

http://id.wikipedia.org/wiki/Actinobacteria

http://id.wikipedia.org/wiki/Bacteria



!#"ynebacte"ium diphthe"iae

Fa+#a#!) a'aer*&Gra% /*(!#!)

T!-a+ &er+a/(, #!-a+ &er(/*ra, #!-a+

&er$era+ber&e'#+ &a#a'$ 1 "!'$$a 8 ;% -a'

e&ar 0, "!'$$a 0,8 ;%

Ter-a/a# #!$a (&(/e(!e( ya'$ -!+e'a

ya+'!> C. diphtheriae mitis, C.diphtheriae intermedius, -a' C.diphtheriae gravis



BAHAN PEMERIKSAAN

Ba"a' /e%er!+(aa' ya'$ &!a(a-!/a+a! '#+ !(*a(!Corynebacterium diphtheriae,

adalah:A/(a' #e'$$*r*+ -a' a#a/(e-*%e%&ara' /a-a-!)#er! #e'$$*r*+



Me-!%Me-!% Ler( ya'$ #er&a# -ar! (er% e%&.

Per#%&"a' Corynebacterium diphtheria /a-a%e-!% !'! 5e/a# -a' &a(! ya'$ #%&"%e'$a'-'$ &a'ya+ &#!r *#!'

Me-!% #er!#e ya'$ 1J #er!#e ya'$ (ee+#!) '#+

Corynebacterium diphtheria. Ba+#er! !'! #%&"(e&a$a! +**'! ya'$ &er&e-a, (e"!'$$a &a+#er! !'!-!&a$! a#a( #!/e +**'! $ra!(, %!#!( -a' !'#er%e-!(. T!/e Gra!(> +**'! &e(ar, -a#ar, &er@ar'a a&

a& (a%/a! "!#a% -e'$a' /er%+aa' ya'$ +er", T!/e M!#!(> +**'! &er+ra' (e-a'$, &er@ar'a

e&!" "!#a%, %e'$+!a/, -a' e&!" +*'e+( ,

T!/e I'#er%e-!(> (a'$a# +e5! ya'$ &!(a +a(ara#a !5!'



Loefer medium Tellurite agar



Corynebacterium diphtheriae a-aa"&a(! /*(!#!) Gra% ya'$ #!-a+ &er(/*ra,#!-a+ &er$era+. B!a(a'ya #er((' -aa%&e-a a#a &er#!$a %e%&e'#+ "r) ,

T, Y, L. Pa-a /e@ar'aa' +"(( &!(a-!!"a# a-a'ya &#!r&#!r *#!' -aa%(!#*/a(%a'ya B#!ra' ya'$ +aya a+a')*()*rCPe@ar'aa' ya'$ &!a(a -!/a+a! '#+

%e!"a# Corynebacterium diphtheriae a-aa" 5ara /e@ar'aa' Ne!((er.



7AMUR

DERMATOFITA

Der%a#*#a> $**'$a' <a%r ya'$-/# %e'5er'a +era#!' -$' e'z!%

+era#!'a(e Tr!5"*/"y#*', (/

M!5r*(/*r%, (/

E/!-er%*/"y#*', (/



DERMATOFITOSIS

Der%a#*#*(!(> !')e+(! *e" <a%r-er%a#*#a /a-a <ar!'$a' y$%e'$a'-'$ +era#!' +!#, ra%&#,++C

S/e(!e( -a' *+a(! !')e+(!>

Ra%&#> M!5r*(/*r%, Tr!5"*/"y#*'

K!#> Tr!5"*/"y#*', M!5r*(/*r%,E/!-er%*/"y#*'

K+> Tr!5"*/"y#*', E/!-er%*/"y#*',

M!5r*(/*r%



6OIL SPIRAL HYFAC



$"ich#phyt#n"ub"um

M!+r*+*'!-!a> (e/er#! #e#e(a' a!r%a#a -! (e+!#ar "y)a

Ma+r*+*'!-!a> &!a(a #!-a+ a-a, +aaa-a &er-!'-!'$ #!/!( -a' !5!'



Tr!5*/"y#*' #*'(ra'

Ga%&ara' K**'!>

Berar!a(! -aa% &e'#+ -a' @ar'a

K**'! &!(a #a%/a+ (e/er#!

$a%&ara' +!# ya'$ 'a+, (e/er#!&e-r a#a (e/er#! &e-a+, #a%/a+"a( -e'$a' /e'!'$$!a' &a$!a'#e'$a"'ya a#a #a%/a+ &er#%/+#%/+

ar'a'ya &erar!a(! %a! -ar!/#!", +'!'$ /5a#, +'!'$ ()r,

5*+a# a#a '$.



Tr!5*/"y#*' r&r%

A-a 2 #!/e

1. D*@'y #y/eP#!", Ha(,6e'#ra U%&*

. Gra'ar #y/e(e/er#! &e-a+

. Mea'*!- Ty/e&er#%/+

2. A)r!5a' Ty/e"a( #e/! ra#a.



Tr!5*/"y#*' Me$'!'!!

K**'! ra#a -a'"a(, &er#%/+#%/+ -a'&er!/a# a#a

&erar (e5arara-!a



Microsporum gypseum

K**'! &er@ar'a

/#!" (e/er#! @*,+e%-!a' (e/er#!&e-a+ "!'$$a&#!ra' -a'

&r@ar'a+e+'!'$a' "!'$$a+e5*+a#a'



Microsporum gypseum



M!5r*(/*r% 6a'!(



K**'!'ya &a'ya+ &e'#+, (e/er#!+a#' -a' &er@ar'a +e+'!'$a'



K**'! &er&e'#+ (e/er#! *#a+

5ere&r!)*r%C /a-a &a$!a'#e'$a"'ya -a' &er!/a# +e ara"

#e/!.

ar'a'ya (e/er#! @ar'a +!#,+'!'$ +e5*+a#a' "!'$$a "!<a

+e+'!'$a'.



N*' -er%a#*#a

Non-DermatotaP!#yr*(/*r% *r&!5areMaa(ez!a

)r)rC +!# T!'ea e(!+**r

P!e-ra!a "*r#aera%&# P!e-ra "!#a% Tr!5"*(/*r*' &e!$e!!ra%&# P!e-ra

/#!"

6a(-*/*r!% @er'e5+!! +!# T!'ea

'!$ra



P!#yr!a(!( er(!5**r

P!#yr!a(!( er(!5**r ("*@!'$ "y/er "y/"*/!$%e'#e- e(!*'(

Malazzesia furfur



Malazzesia furfur



PIEDRA

P!e-ra "!#a%> E#!**$! > P!e-ra!a "*r#ae

Ra%&# +e/aa, '*- "!#a%5*+a#, +era(%ee+a# era#, ra%&# -a/a# /a#a"

P!e-ra /#!">

E#!**$!> Tr!5"*(/*r*' &e!$e!!

N*- /#!"+re%, 'a+



Piedra hortae



PIEDRA PUTIH



ASPERGILLUS

Pe'ya+!# <a%r A(/er$!*(!(C ya'$ %'5-e'$a' &er&a$a! (!'-r*%a +!'!( ya'$-!(e&a&+a' *e" (/e(!e( A(/er$!(.Pe'-er!#a -e'$a' /e'ya+!# /ar +r*'!(

#er#a%a a(#"%a, <$a /e'ya+!# $a'$$a'/ar +r*'!( a#a 5y(#!5 &r*(!(C -a'/e'-er!#a ya'$ aer$! #er"a-a/ <a%r !'!-a/a# %e'ye&a&+a' +er(a+a' &r*'5"(

-a' /e'y%&a#a' &r*'5"( !'#er%!#e'.Kea-aa' !'! -!(e&# (e&a$a! aer$!5&r*'5"*/%*'ary a(/er$!*(!( ABPAC.



A(/er$!*(!( ya'$ !'a(!) -a/a##er<a-!, #er#a%a /a-a /a(!e' ya'$%e'er!%a #era/! !%'*(/re(!) a#a(!#*#*+(!+ !a -a/a# %e'ye&ar +e *#a+,$!'<a -a' *r$a' a!' -a' (er!'$+a!)a#a. I'a(! +e-aa% /e%&" -ara"&er/a #r*%&*(!( -a' %e'ye&a&+a'

!')ar+ a-aa" 5!r! -ar! !')e+(! <a%r !'!/a-a /a(!e' -e'$a' +e+e&aa're'-a".



My5*(!( Sy(#e%!5



A (e +a+!

B K*'!-!a)*r

6 e(!+e

D S#er!$%a#a

E K*'!-!a

& ill



&%pe"gillu% 'enicillium



spergillus



PENI6ILLIUM

!amur peng"a#il peni#ilin

Peni#ilin a-aa" (e&a"+e*%/*+ a'#!&!*#!+a Qa+#a%ya'$ -!$'a+a' -aa%/e'ye%&"a' /e'ya+!# !')e+(!+are'a &a+#er!, &!a(a'ya&er<e'!( Gra% /*(!#!) .

http://id.wikipedia.org/wiki/Antibiotika

http://id.wikipedia.org/wiki/Bakteri

http://id.wikipedia.org/wiki/Gram_positif


http://id.wikipedia.org/wiki/Penisilin

http://id.wikipedia.org/wiki/Penisilin



http://id.wikipedia.org/wiki/Bakteri

http://id.wikipedia.org/wiki/Antibiotika



Penicillium



TERIMA KASIH

“aka apabila kamu telah %ele%ai (da"i %e%uatuu"u%an), ke"akanlah dengan %ungguh*%ungguh

praktikum tropis

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