mikrobiologi JAMUR
7
LEMBAR KERJA PRAKTIKUM PEMERIKSAAN JAMUR TUJUAN Mengamati gambaran mikroskopik jamur dan membedakannya dari bakteri Mengamati gambaran biakan jamur pada agar Sabouraud Memahami dan mengerjakan pembuatan preparat dan pengecatan jamur dengan cat Gram dan LPCB, dari spesimen jamur yang telah disediakan. DASAR TEORI Tulislah dasar teori berdasarkan pustaka sebanyak 150-200 kata. ALAT Mikroskop Gelas obyek Deck glass Ose bulat Kertas label BAHAN Spesimen untuk pemeriksaan jamur Contoh preparat mikroskopik Candida sp Contoh biakan jamur pada agar Sabouraud : C. al bicans, Rhizop us sp, Trichoph yton sp, Trichophy ton rubrum, Aspergillu s fumigatu s , Aspergillus flavus, Aspergillus niger Cat Gram A, B, C dan D Cat LPCB KOH 10-20 % Laboratorium Mikrobiologi FK UII 1
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Transcript of mikrobiologi JAMUR
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LEMBAR KERJA PRAKTIKUMPEMERIKSAAN JAMUR
TUJUAN
Mengamati gambaran mikroskopik jamur dan membedakannya dari bakteri
Mengamati gambaran biakan jamur pada agar SabouraudMemahami dan mengerjakan pembuatan preparat dan pengecatan jamur dengancat Gram dan LPCB, dari spesimen jamur yang telah disediakan.
DASAR TEORI
Tulislah dasar teori berdasarkan pustaka sebanyak 150-200 kata.
ALAT
MikroskopGelas obyekDeck glassOse bulatKertas label
BAHAN
Spesimen untuk pemeriksaan jamur Contoh preparat mikroskopik Candida spContoh biakan jamur pada agar Sabouraud : C. albicans, Rhizopus sp,
Trichophyton sp, Trichophyton rubrum, Aspergillus fumigatus, Aspergillusflavus, Aspergillus niger
Cat Gram A, B, C dan DCat LPCBKOH 10-20 %
Laboratorium Mikrobiologi FK UII 1
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SKEMA KERJA
Pemeriksaan Jamur
0100090000032a0200000200a20100000000a201000026060f003a03574d4643010000
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
000080000000800004300000001000000000000006801000025000000372e90010000020f0502020204030204ef0200a07b20004000000000000000009f00000000000000430061006c0069006200720000000000000000006ca84200dee31e6de88df86dccab4200d8a742009c38176d070000000100000014a8420014a84200e878156d070000003ca84200fc028c006476000800000000250000000c00000001000000250000000c00000001000000250000000c00000001000000180000000c00000000000002540000005400000000000000000000001f0000004200000001000000e4b9e140d6bee1400000000034000000010000004c000000040000000000000000000000d8090000f10e00005000000020001f002000000046000000280000001c0000004744494302000000ffffffffffffffffd9090000f20e0000000000004600000014000000080000004744494303000000250000000c0000000e000080250000000c0000000e0000800e000000140000000000000010000000140000000400000003010800050000000b0200000000050000000c02fe02f801040000002e0118001c
000000fb020300010000000000bc02000000000102022253797374656d00004d53205368656c6c20446c67000000000000000000000000040000002d010000040000002d01000004000000020101001c000000fb02f5ff0000000000009001000000000440002243616c6962726900000000000000000000000000000000000000000000000000040000002d010100040000002d010100040000002d010100050000000902000000020d000000320a0a0000000100040000000000f701fd0220000600040000002d010000040000002d010000030000000000
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* Pengecatan LPCB
0100090000032a0200000200a20100000000a201000026060f003a03574d46430100000000000100c9a40000000001000000180300000000000018030000010000006c00000000000000000000001f000000420000000000000000000000744500006c69000020454d46000001001803000012000000020000000000000000000000000000004c0b00001d100000cc000000230100000000000000000000000000000f1d0300e2700400160000000c
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
fc028c006476000800000000250000000c00000001000000250000000c00000001000000250000000c00000001000000180000000c00000000000002540000005400000000000000000000001f0000004200000001000000e4b9e140d6bee1400000000034000000010000004c000000040000000000000000000000d8090000f10e00005000000020001f002000000046000000280000001c0000004744494302000000ffffffffffffffffd9090000f20e0000000000004600000014000000080000004744494303000000250000000c0000000e000080250000000c0000000e0000800e000000140000000000000010000000140000000400000003010800050000000b0200000000050000000c02fe02f801040000002e0118001c000000fb020300010000000000bc02000000000102022253797374656d00004d53205368656c6c20446c67000000000000000000000000040000002d010000040000002d01000004000000020101001c000000fb02f5ff0000000000009001000000000440002243616c6962726900000000000000000000000000000000000000000000000000040000002d01
0100040000002d010100040000002d010100050000000902000000020d000000320a0a0000000100040000000000f701fd0220000600040000002d010000040000002d010000030000000000
Membuat mikrokultur (slide culture)
Laboratorium Mikrobiologi FK UII 3
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0100090000032a0200000200a20100000000a201000026060f003a03574d46430100000000000100c9a40000000001000000180300000000000018030000010000006c00000000000000000000001f000000420000000000000000000000744500006c69000020454d46000001001803000012000000020000000000000000000000000000004c0b00001d100000cc000000230100000000000000000000000000000f1d0300e2700400160000000c000000180000000a0000001000000000000000000000000900000010000000d8090000f10e0000250000000c0000000e000080250000000c0000000e000080120000000c00000
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
30000000000Tugas praktikan :
Membuat preparat dari spesimen jamur yang tersedia (2 spesimen)Melakukan pengecatan preparat dengan cat Gram dan LPCBMenggambar hasil contoh preparat mikroskopik Candida spMenggambar hasil contoh biakan jamur pada agar Sabouroud: C. Albicans, Rhizopus
sp, Trichophyton sp, Trichophyton rubrum, Aspergillus fumigatus, Aspergillusflavus, Aspergillus niger
HASIL
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Laboratorium Mikrobiologi FK UII 5
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PEMBAHASAN
KESIMPULAN
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