LAPORAN HASIL PENELITIAN

PROGRAM RESEARCH GRANT I-MHERE JURUSAN

TAHUN ANGGARAN 2010

Judul : ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT INDIGENOUS PENGHASIL ANTIBAKTERI

DARI FESES BAYI Ketua :Dr. Ir. Joni Kusnadi, MSi. Anggota :Tunjung Mahatmanto, STP, MSi.

Dibiayai Oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan Nasional, melalui DIPA Universitas Brawijaya berdasarkan SK Rektor Nomor :

214/SK/2010, tanggal 22 Juli 2010.

UNIVERSITAS BRAWIJAYA NOPEMBER

2010

THP

RINGKASAN

Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi dan mengkarakterisasi

bakteri asam laktat bersifat anaerob yang menghasilkan senyawa antimikroba dari

feces bayi. Metode yang digunakan dalam penelitian ini adalah eksploratif deskriptif.

Dari feces bayi yang hanya mengkonsumsi ASI dilakukan isolasi bakteri secara

anaerob, dan didapatkan 5 isolat yang mempunyai aktivitas antimikroba pada

supernatan bakteri yang pH-nya sudah dinetralkan. Hasil identifikasi dengan API 50

CHL, ke-lima bakteri tersebut menunjukkan kedekatan dengan persentase identitas

99,2% mendekati bakteri Lactobacillus paracasei.

Kata Kunci : Feses bayi, bakteri asam laktat, anaerob, anti mikroba

SUMMARY

The aim of this research was to isolate, identify and to characterize anaerobic lactic

acid bacteria isolated from infant feces which have capability to produce

antimicrobial compound. Explorative descriptive method was in this experimental

work. From infant feces who consumed only ASI, 5 isolates of lactic acid bacteria

confering antimicrobial activity were succesfully isolated. Further identification using

API 50 C HL test kit showed that all 5 isolates were Lactobacillus paracasei with

percentage identity 99,2%.

Key Word : Infant feces, lactic acid bacteria, anaerobic, antimicrobial

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