29

LAMPIRAN

Lampiran 1. Gambar Tempe Satu kali Perebusan dengan Berbagai Perbandingan Kedelai Lokal dan Impor

Tempe kedelai lokal 100% Tempe kedelai lokal:impor = 75% : 25%

Tempe kedelai lokal:impor = 50% : 50% Tempe kedelai lokal:impor = 25%: 75%

Tempe kedelai impor 100%

30

Lampiran 2. Hasil Pengukuran Kadar Air Tempe

Ulangan

Persentase(Lokal : Impor)

Kadar Air (%)

I II III IV V Rata-rata

100% : 0%64,48 63,75 63,43 64,54 64,48

64,14

75% : 25%63,72 63,16 63,34 64,23 64,41 63,77

50% : 50%63,69 63,05 63,00 64,01 63,73 63,44

25% : 75%63,39 62,81 62,76 63,98 63,42 63,27

0% : 100%62,69 62,59 62,62 62,66 62,68 62,65

31

Lampiran 3. Hasil Pengukuran Absorbansi Sampel

Tabel 3.1. Absorbansi Aktivitas Antioksidan Tempe

Ulangan

Konsentrasi(Lokal : Impor)

Absorbansi ( λ = 700 nm)

I II III IV V

100% : 0% 0,843 0,873 0,806 0,850 0,8369

75% : 25% 0,831 0,847 0,794 0,847 0,819

50% : 50% 0,828 0,828 0,786 0,826 0,813

25% : 75% 0,818 0,797 0,731 0,795 0,773

0% : 100% 0,770 0,786 0,726 0,789 0,763

Tabel 3.2. Absorbansi Kadar Fenolik Total Tempe

Ulangan

Konsentrasi(Lokal : Impor)

Absorbansi ( λ = 765 nm)

I II III IV V

100% : 0% 0,873 0,882 0,823 0,878 0,860

75% : 25% 0,868 0,869 0,813 0,861 0,847

50% : 50% 0,843 0,842 0,808 0,843 0,825

25% : 75% 0,822 0,811 0,799 0,833 0,803

0% : 100% 0,803 0,773 0,751 0,825 0,736

32

Lampiran 4. Kurva Standar K4Fe(CN)6

Contoh Perhitungan Aktivitas Antioksidan Tempe

Sampel : Tempe Kedelai Lokal 100 % (Ulangan I)Persamaaan Kurva Standar: y = 0,369 x + 0,047Absorbansi (700 nm) = 0,843

0,843 = 0,369 x + 0,0470,369 x = 0,843 – 0,047

x =

x = 2,1572 mM

Konsentrasi(mM)

Absorbansi (700nm)

0,2710,5430,8141,0861,3571,6291,9002,1722,4432,715

0,1240,2510,3570,4620,5660,6740,7060,8560,9501,049

33

Lampiran 4 (Lanjutan)

Dalam 100 ml = 0,1 L x 2,1572 mM = 0,21572 mek / 5,01 gram

= 4,306 x 10-2 mek / gram berat basah

Kadar Air Sampel = 64,48 %Berat basah sampel = 5,01 gram, maka

Berat air =

Berat kering sampel = (5,01 – 3,2304) gram = 1,7796 gram

Berdasarkan berat kering =

= 12,1218 x 10-2 mek / gram

34

Lampiran 5. Kurva Standar Asam Galat

Contoh Perhitungan Kadar Fenolik Total Tempe

Sampel : Tempe Kedelai Lokal 100 % (Ulangan I)Persamaaan Kurva Standar: y = 9,724 x – 0,009Absorbansi (765 nm) = 0,873

0,873 = 9,724 x -0,0099,724 x = 0,873 – 0,037

x =

x = 9,0703 x 10-2 mg/ml

Konsentrasi (mg/ml)

Absorbansi ( 765 nm)

0,01 0,0810,02 0,1880,03 0,2650,04 0,3960,05 0,4960,06 0,5680,07 0,6560,08 0,7720,09 0,8740,1 0,954

35

Lampiran 5 (Lanjutan)

Dalam 100 ml = 100 x 9,0703 x 10-2 mg/ml

= 9,0703 mg

Kadar Air Sampel = 64,48 %Berat basah sampel = 5,01 gram, maka

Berat air =

Berat kering sampel = (5,01 – 3,2304) gram = 1,7796 gram

Berdasarkan berat kering =

= 5,0968 mg / gram

36

Lampiran 6. Hasil Perhitungan Aktivitas Antioksidan Tempe

Ulangan

Persentase(Lokal : Impor)

Aktivitas Antioksidan (mek / gram)

I II III IV V Rata-rata

100% : 0%12,1218x10-2 12,3259x10-2 11,2491x10-2 12,2012x10-2 11,9651x10-2 11,9726 x10-2

75% : 25%11,7128x10-2 11,7462x10-2 11,0442x10-2 12,0975x10-2 11,6864x10-2 11,6574 x10-2

50% : 50%11,6113x10-2 11,4331x10-2 10,8254x10-2 11,6616x10-2 11,4242x10-2 11,3911 x10-2

25% : 75%11,4144x10-2 10,9304x10-2 9,9357x10-2 11,2106x10-2 10,6935x10-2 10,8369 x10-2

0% : 100%10,5028x10-2 10,7068x10-2 9,8060x10-2 10,7489x10-2 10,3781x10-2 10,4285 x10-2

37

Lampiran 7. Hasil Perhitungan Kadar Fenolik Total Tempe

Ulangan

Konsentrasi(Lokal : Impor)

Kadar Fenolik Total ( mg / gram)

I II III IV V Rata-rata

100% : 0%5,0968 5,0454 4,6793 5,1142 4,9819

4,9835

75% : 25%4,9718 4,8920 4,6117 4,9924 4,9173

4,87704

50% : 50%4,8068 4,7275 4,5416 4,8399 4,7119

4,72714

25% : 75%4,6686 4,5349 4,4537 4,7887 4,5385

4,59688

0% : 100%4,4763 4,2994 4,1650 4,5848 4,0978

4,32466

38

Lampiran 8. Analisa Data Aktivitas Antioksidan Tempe

UlanganPersentase Kedelai (Lokal : Impor)

Tu Xu du W100%:0% 75%:25% 50%:50% 25%:75 0%:100%

1 0,1212 0,1171 0,1161 0,1141 0,105 0,5736 0,1147 0,0021 0,000141

2 0,1233 0,1175 0,1143 0,1093 0,1071 0,5714 0,1143 0,0014 0,000158

3 0,1125 0,1104 0,1083 0,0994 0,0981 0,5286 0,1057 -0,0069 0,000161

4 0,122 0,1209 0,1166 0,1121 0,1075 0,5792 0,1158 0,0033 0,000151

5 0,1197 0,1169 0,1162 0,1069 0,1038 0,5615 0,1123 0,0001 0,000167

Tp 0,5986 0,5829 0,5696 0,5418 0,5214 2,8143

Xp 0,1197 0,1166 0,1139 0,1084 0,1043 (X…) 0,1126

dp 0,007153 0,004001 0,0013388 -0,004204 -0,008288

SD 0,00425 0,00381 0,0033333 0,005733 0,003794

SE 0,00528 0,00473 0,00414 0,00712 0,00471

39

Lampiran 8 (Lanjutan)

W1= {(0,1212 x 0,007153) + (0,1171x 0,004001) + (0,1161x0,0013388) + (0,1141x-0,004204) + (0,105x-0,008288)} = 0,000141

W2= {(0,1233x 0,007153) + (0,1175x 0,004001) + (0,1143x0,0013388) + (0,1093x-0,004204) + (0,1071x-0,008288)} = 0,000158

W3= {(0,1125x 0,007153) + (0,1104x 0,004001) + (0,1083x0,0013388) + (0,0994x-0,004204) + (0,0981x-0,008288)} = 0,000161

W4= {(0,122x 0,007153) + (0,1209x 0,004001) + (0,1166x0,0013388) + (0,1121x-0,004204) + (0,1075x-0,008288)} = 0,000151

W5= {(0,1197x 0,007153) + (0,1169x 0,004001) + (0,1162x0,0013388) + (0,1069x-0,004204) + (0,1038x-0,008288)} = 0,000167

N = Wi x du={(0,000141x0,0021) + (0,000158x 0,0014) + (0,000161x -0,0069) + (0,000151x 0,0033) + (0,000167x0,0001) = -8,3 x 10-8

D = du2 x dp2

= {[(0,0021)2 + (0,0014)2 + (-0,0069)2 + (0,0033)2 + (0,0001)2] x [(0,007153)2 + (0,004001)2 + (0,0013388)2 + (-0,004204)2 + (-0,008288)2]} = 1,0138 x 10-8

FK = = = 31,6818 x 10-2

JK Non Aditivitas = = = 7,0147x 10-7

JK Total = p2 – FK

= {(0,1212)2 + (0,1171)2 + …. +(0,1038)2} -31,6818 x 10-2

= 31,7959 x 10-2 - 31,6818 x 10-2

= 11,4056 x 10-4

JK Ulangan = - FK

= – 31,6818 x 10-2 = 3,2634 x10-4

40

Lampiran 8 (Lanjutan)

JK Perlakuan = - FK

= – 31,6818 x 10-2 = 7,7664 x 10-4

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan

= (11,4056 x 10-4) - (3,2634 x 10-4) – (7,7664 x 10-4)

= 3,7581 x 10-4

DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F HitungF tabel

5% 1%

Ulangan 4 3,2634 x 10-4

Perlakuan 4 7,7664 x 10-4

Galad Acak 16 3,7581 x 10-5

Non Additivitas 1 7,0147 x 10-7 7,0147 x 10-7 0,2853 4,53 8,68

Sisa 15 3,6879 x 10-5 2,4587 x 10-7

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif

DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F HitungF tabel

5% 1%

Ulangan 4 3,2634 x 10-4 8,1584 x 10-5 34,7341** 3,01 4,77

Perlakuan 4 7,7664 x 10-4 1,9416 x 10-4 82,6628** 3,01 4,77

Galad Acak 16 3,7581 x 10-5 2,3488 x 10-6

Total 24

**Antar ulangan dan perlakuan berbeda sangat nyata

41

Lampiran 8 (Lanjutan)

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α (p,n2)Sx

= 1,36 % Sx = = 6,8557 x 10-4

BNJ 5 % = 4,34 x (5,7446 x 10-4)

= 2,9754 x 10-3

Aktivitas Antioksidan (mek / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0%

Purata

± SE0,1043

± 0,00470,1084

± 0,00710,1139

± 0,00410,1166

± 0,00470,1197

± 0,0053

W = 2,9754 x10-3 (a) (b) (c) (c) (d)

42

Lampiran 9. Analisa Data Kadar Fenolik Total Tempe

UlanganPersentase Kedelai (Lokal : Impor)

Tu Xu du W100% L 75% L 50% L 25% L 100% I

1 5,0968 4,9718 4,8068 4,6686 4,4763 24,0203 4,8041 0,1022 0,249759

2 5,0454 4,892 4,7275 4,5349 4,2994 23,4992 4,6998 -0,0020 0,300054

3 4,6793 4,6117 4,5416 4,4537 4,165 22,4513 4,4903 -0,2116 0,202343

4 5,1142 4,9924 4,8399 4,7887 4,5848 24,3200 4,8640 0,1622 0,205574

5 4,9819 4,9173 4,7199 4,5385 4,0978 23,2554 4,6511 -0,0508 0,362071

Tp 24,9176 24,3852 23,6357 22,9844 21,6233 117,5462

Xp 4,9835 4,8770 4,7271 4,5969 4,3247 (X…) 4,7018

dp 0,2817 0,1752 0,0253 -0,1049 -0,3772

SD 0,1777 0,1537 0,1157 0,1319 0,2051

SE 0,2206 0,1909 0,1436 0,1639 0,2546

43

Lampiran 9 (Lanjutan)

W1= {(5,0968x 0,2817) + (4,9718x 0,1752) + (4,8068x0,0253) + (4,6686x-0,1049) + (4,4763x-0,3772)} = 0,249759

W2= {(5,0454x 0,2817) + (4,892x 0,1752) + (4,7275x0,0253) + (4,5349x-0,1049) + (4,2994x-0,3772)} = 0,300054

W3= {(4,6793x 0,2817) + (4,6117x 0,1752) + (4,5416x0,0253) + (4,4537x-0,1049) + (4,165x-0,3772)} = 0,202343

W4= {(5,1142x 0,2817) + (4,9924x 0,1752) + (4,8399x0,0253) + (4,7887x-0,1049) + (4,5848x-0,3772)} = 0,205574

W5= {(4,9819x 0,2817) + (4,9173x 0,1752) + (4,7199x0,0253) + (4,5385x-0,1049) + (4,0978x-0,3772)} = 0,362071

N = Wi x du={(0,249759 x0,1022) + (0,300054 x -0,0020) + (0,202343 x -0,2116) + (0,205574 x 0,1622) + (0,362071 x-0,0508) = -2,9348 x 10-3

D = du2 x dp2

= {[(0,1022)2 + (-0,0020)2 + (-0,2116)2 + (0,1622)2 + (-0,0508)2] x [(0,2817)2

+ (0,1752)2 + (0,0253)2 + (-0,1049)2 + (-0,3772)2]} = 22,1968 x 10-3

FK = = = 552,6844

JK Non Aditivitas = = = 3,8803 x 10-4

JK Total = p2 – FK

= {(5,0968)2 + (4,9718)2 + …. +(4,0978)2} -552,6844

= 554,5165 - 552,6844

= 1,8321

JK Ulangan = - FK

= – 552,6844 = 4,2046 x10-1

45

Lampiran 9 (Lanjutan)

JK Perlakuan = - FK

= – 552,6844= 1,3198

JK Galad Acak = JK Total – JK Ulangan – JK Perlakuan

= (1,8321) - (4,2046 x10-1) – (1,3198)

= 9,1861 x 10-2

DASIRA UJI ADDITIVITAS

Sumber Ragam Db JK KT F HitungF tabel

5% 1%

Ulangan 4 4,2046 x 10-1

Perlakuan 4 1,3198

Galad Acak 16 9,1861 x 10-2

Non Additivitas 1 3,8803 x 10-4 3,8803 x 10-4 0,0636 4,53 8,68

Sisa 15 9,1473 x 10-2 6,0982 x 10-3

F Hitung Non Additivitas < F Tabel 5% dan 1% Data Additif

DAFTAR SIDIK RAGAM

Sumber Ragam Db JK KT F HitungF tabel

5% 1%

Ulangan 4 4,2046 x 10-1 0,1051 18,3085** 3,01 4,77

Perlakuan 4 1,3198 0,3299 57,4697** 3,01 4,77

Galad Acak 16 9,1861 x 10-2 0,0057

Total 24

**Antar ulangan dan perlakuan berbeda sangat nyata

Lampiran 9 (Lanjutan)

46

KV = x 100% Uji Beda Nyata Jujur (BNJ) 5%

= x 100% BNJ 5 % : W = q α (p,n2)Sx

= 1,61 % Sx = = 3,3764 x 10-2

BNJ 5 % = 4,34 x (3,6530 x 10-2)

= 0,1465

Kadar Fenolik Total (mg / gram)

0%:100% 25%:75% 50%:50% 75%:25% 100%:0%

Purata± SE

4,3247± 0,2546

4,5969± 0,1639

4,7271± 0,1436

4,8770± 0,1909

4,9835± 0,2206

W = 0,1465 (a) (b) (bc) (cd) (d)