ADDENDUM SPESIFIKASI TEKNIS PENGADAAN PERALATAN LABORATORIUM (LELANG ULANG)SUMBER DANA : APBN TAHUN ANGGARAN 2012Perubahan dalam spesifikasi teknis pengadaan peralatan laboratorium :1. Item No. 27 (Automatic Serial Soxhlet System), Chiller, Footprint : tertulis 210 x 450 x570 mm, seharusnya 210 x 400 x500 mm or greater2. Item No. 29 (Rotary Vacuum Evaporator + Chiller), Recirculating Chiller, Footprint of chiller : 280 x 400 x 500 mm or greater, seharusnya 210 x 400 x 500 mm or greater3. Item No. 39 (Distillation Unit) dan 40 (Distillation Unit For Analisys SO2 With Titrator), tertulis Protective door with sensor, seharusnya Protective door (tulisan with sensor dihilangkan)4. Item No. 54 (Cabinet Sterilizing) Perubahan Dimensions tertulis at least 860 x 480 x 1300 mm, seharusnya at least 860 x 490 x 1750 mm

NO INSTRUMEN NAME and TECHNICAL SPECIFICATION ENTITY AMOUNT MEREK / TYPE1. ATOMIC ABSORPTION SPECTROPHOTOMETER *) Unit 1

Specifications :

a. Analysis methods : Flame & Furnaceb. Optic system : : double-beam methodc. Measured wavelength range : 185 - 900 nm or greaterd. Detector : Photomultiplier or equivalente. Background correction : D2 and Zeeman methodf. lamps : 8-lampsg. Gas Controllers : Automatich. Burner head : Titanium or the corotion resistant materiali. furnace (1 unit )

- autosampler- Programming temperature : 40 to 3000° C

Includes of:

a. Training b. Original Operasional manual (plus bahasa indonesia)c. Guarantee min. A yeard. Installatione. Factory Certificatef. Blower + Vent + Dusting (2 set)g. Vapor Generator accessory (pump units; gas liquid separator; quartz cell;

reagent container; tubing, Flow Through Mercury Cell and spares kit)h. Hollow Cathode Lamps + Standard Solution for : Pb, Hg, Cd, Zn, As, Mg, Ca, Na,

K,Cu, Fe, Cr, Se, Sn, Ag (15 buah)i. Argon gas with tank & regulator (1 set)j. C2H2 gas with tank & regulator (1 set)k. N20 gas with tank & regulator (1 set)l. Compressor + air dryer (1 set) m. UPS 10 KVA (2 unit)n. Dust cover + tool kit (1 unit)

o. Furnace Camera (1 unit)p. Recirculating Water Chiller (1 unit)q. PC + Software + printer (1 set)r. Online Calibration & Dilution System

2 LSMSMS *) Unit 1

Specifications :MASS SPECTROMETRY

a. Ion Sources : ESI dan APCIb. Ion Sources Transfer Optics : High Efficiency stacked ring ion guidec. Detector : Photomultiplier or equivalentd. Vacuum System : singlee. Mass range : 2 to 2000 or widerf. Scan Speed : 5000 - 10000 Da/sg. Mass resolution : ≤ 1,0 Da FWHM

h. Mass sensitivity : ESI(-) , S/N 180:1, 10 pg Reserpine or better

POMPAa. Type : Quartenaryb. Flow rate range : minimum 0,010 to 2,000 ml/minc. Flow rate precision :d. Flow rate accuracy :e. Maximum operating pressure : 8500 psi or greater

AUTOSAMPLERa. Injection volume : 0,1 to 10,0 µLb. Injection precision : ≤ 1 % RSDc. Sampel capacity : min 90 d. Sample carryover : < 0,005 %

OVEN KOLOMColumn temperature range : 20 to 90° C or greater

KOMPUTER DAN SOFTWAREHardware : Min Pentium 4, 3,2 GHz/800MHz, 2 MB L2 Cache, Keyboard &

Scroll Mouse & USB, 160 GB 7200 RPM, Windows XP Pro, DVD Multi Burner, Com 2 cable, System Restore DVD, Monitor, Printer.

Software : Compatible

≤ 0,075 % RSD± 1,0 %

LIBRARY compatible with system

UPS min 6 KVA

Magnetic Switch

Sample Cone

Solvent for 1 year :

Methanol 4 x 2,5 LAcetonitril 2 x 2,5 LNH4OHFormic Acid 1 L

Includes of:

1. Installation2. Training 3. Performance Test4. After Sales Support5. Factory Certificate6. Guarantee 1 year7. Spare Part Availability min. 5 year8. Original manual book9. Column C18 : min 3 pcs

10. Column C8 : min 3 pcs11. Guard column for C 18 : min 3 pkgs12. Guard column for C 8 : min 3 pkgs13. Pre Column Filter Holder (fitting included) : min 1 pk14. 0,2 µm Filter Cartridge, 5 / pk : min 1 pk15. Tube 50 ml /AOAC-acetat : min 1 pk16. Tube 2 ml- AOAC/C18 : min 1 pk17. Solvent Clarification Kit with pump 220 V/50 Hz : min 1 kit18. Membran filter diameter 25 mm, 0,2 µm ; 50 /pk : min 3 pkgs19. Sampel vial min 1 ml (100 pc/pk) : min 3 pkgs

3 GC Auto injector With Detector FID, TCD, FPD *) Unit 1

Each set consist of :1 Coloumn oven : 1 unit2 Injection Port : minimal 1 unit3 Flow Control Unit : 1 unit4 Split / Splitless, Programmable Temperature Vaporization injection unit : 1 unit

N2 generator min 20 L/sec, with internal compressorAr gas + cylinder + regulator, tubibg, connector, reducer

5 Detectors : 3 unit6 Workstation : 1 set7 Autoinjektor : minimal 1 unit

1 Column OvenSpecifications :

a. Temperature range : to 450 C⁰b. Cool down range : up to 50 C or more cooler⁰

2 Injection Port : minimum 1Specifications :Split/Splitless, Programable Temperature Vaporization Injection Unit

- Split ratio setting range : 1:7500 or better- Total Flow setting : 0 - 200 mL/min or better- Temperature range : up to 400 C or greater⁰

3 Flow Control UnitSpecification :

a. Pressure setting range : 0 to 100 psi or greaterb. Pressure control precision : 0,001 psi or greater

4 DetectorsFlame Ionization Detector (FID)Specification :

a. Temperature range : up to 400°C or greater

Thermal Conductivity Detector (TCD)Specification :

a. Temperature range :

Flame Photometric Detector (FPD)Specification :

a. Temperature range :

5 a. HardwareMinimum Intel Processor core I3, Windows XP, Hard Disk min. 120 GB, RAM min 2 GB, CDROMDrive, DVD/CD Writer, LCD Monitor 17", Color printer.

b. Software :- Compatible with Windows XP

Up to 400oC or greater

Up to 350oC or greater

- Data Acquisition- Hardware Functions- Data Processing and Analysis- Data Storage

7 Autoinjector a. number of vials : min 12 or greater

8 Accessories :

1. Hydrogen gas : 1 pcs2. Helium Gas : 1 pcs3. Compress Air : 1 pcs4. Regulator for gases : 3 pcs5. 10 mL screw vial : 1 pcs6. Capillary Column : 2 pcs7. UPS min 5 KVA : 1 pcs8. GC Data Systems : 1 pcs9. Driver for SW : 1 pcs

10. Gas Clean Filter : 1 pcs

9. The system includes of :

1. Instalation

2. Training

3. Dust Cover

4. Guarantee min. 1 year

5. Spare Part Availability min. 5 year

6. Original operation manual

7. Factory Certificate8. Tool Kit9. BAPETEN license for ECD

10. Training at Representative GC Training Centre

4 GC Auto injector With Detector FID, TCD, ECD *) Unit 1

Each set consist of :

1. Coloumn oven : 1 unit2. Injection Port : min 1 unit3. Flow Control Unit : 1 unit4. Split / Splitless, Programmable Temperature Vaporization injection unit : 1 unit5. Detectors : 3 unit6. Workstation : 1 set

7. Autoinjektor : min 1 unit

1 Column Oven

Specifications :

a. Temperature range : to 450 C⁰b. Cool down rate : up to 50 C or more cooler⁰

2 Injection Port : minimum 1Specifications :

Split/Splitless, Programable Temperature Vaporization Injection Unita. Split ratio setting range : 1:7500 or betterb. Flow rate setting range : 0 -200 mL/min or betterc. Temperature range : up to 400 C or greater⁰

3 Flow Control Unit

Specification :

a. Pressure setting range : 0 to 100 psi or greaterb. Pressure control precision : 0,001 psi or greater

4 Detectors

Flame Ionization Detector (FID)

Specification :

a. Temperature range : up to 400°C or greater

Thermal Conductivity Detector (TCD)

Specification :

a. Temperature range :

Electron Capture Detector (ECD)

Specification :

a. Temperature range :

5 a. Hardware

Minimum Intel Processor core I3, Windows XP, Hard Disk min. 120 GB, RAM min 2 GB, CDROM Drive, DVD/CD Writer, LCD Monitor 17", Color printer.

b. Software :

a. Compatible with Windows XP b. Data Acquisitionc. Hardware Functions

Up to 400oC or greater

Up to 350oC or greater

d. Data Processing and Analysise. Data Storage

7 Autoinjector

a. number of vials : min 12 or greater

8 Accessories :

1. Hydrogen gas : 1 pcs2. Helium Gas : 1 pcs3. Compress Air : 1 pcs4. Nitrogen Gas : 1 pcs5. Regulator for gases : 4 pcs6. 10 mL screw vial : 1 pcs7. Capillary Column : 2 pcs8. UPS min 5 KVA : 1 pcs9. GC Data Systems : 1 pcs

10. Driver for SW : 1 pcs11. Gas Clean Filter : 1 pcs

9. The system includes of :

1. Instalation2. Training3. Dust Cover4. Guarantee min. 1 year5. Spare Part Availability min. 5 year6. Original operation manual7. Factory Certificate8. Tool Kit9. BAPETEN license for ECD

10. Training at Representative GC Training Centre

5 HPLC UV-Vis with Auto injector *) Unit 4

Specifications

Pompa : maximum 2 unit

a. Type : Quaternary b. Flow rate range : 0,0001-5,0 ml/min or greaterc. Flow rate accuracy : ± 1% d. Flow rate precision : ≤ 0.1% RSD e. Maximum pressure : 5000 - 6000 psi

f. Gradient accuracy : ± 1% g. Number of solvent : min 3

Detektor UV-Vis : 1 unit

a. Lamp : D2 lampb. Wavelength range : 200 - 600 nm or greaterc. Wavelength Precision : ≤ 0,1 nmd. Wavelenght Accuracy : ± 1 nme. Linearity : ≤2,5 AUf. Function : Dual Wavelengthg. Noise :

Column Oven With Cooler : 1 unit

Temperature setting range : 4º C to 65º C or higher

Autosampler With Sample Cooler : 1 unit

a. Injection Accuracy : ≤ 2% or betterb. Temperatur control : 4º C - 40º C c. Injection volume : 0,1-100uld. Sample Capacity : min 100 vial @ ± 1 - 2 ml

Degasser : 1 unit

Data Processor : 1 set

- Minimum Core i3 2100, 3.1 GHz, Intel GMA,Win 7 Pro 32-bit, HDD 500 GB 7200RPMSATA, USB Optical Mouse & Keyboard,Media Card Reader, Monitor LCD 18,50", Printer.

- Compatible Software for Data analysis and reporting

Include

1 Instalation2 Training3 Tool kits (1 set)4 Maintenance & autorinsing Kit for pump (1 set)5 Dust Cover6 C18 coloumn, 5µm, 25 cm: minimum 2 pcs7 Sampel vial min 1 ml (100 pc/pk)8

≤ 5 x 10-6 AU

Micro Syringe 100 µL, minimum 1 pcs

9 Compatible mixer, min 1 set10 Guarantee min. 1 year11 Spare Part Availability min. 5 year12 Reservoir Tray (1 pcs)13 Original manual book14 Factory certificate 15 Stabilizer min 3 KVA16 Needle wash (1 set)17 HUB W/ Cable

6 HPLC PDA with Auto injector *) Unit 1

Spesifications

1. Pompa (maksimal 2 unit)

a. Tipe : Quarternary

b. Rentang laju alir : 0,0001-5,0 ml/min or greaterc. Akurasi laju alir : ± 1% d. Presisi laju alir :e. Gradient accuracy :f. Jumlah wadah fase gerak : minimal 3g. Max. Pressure : 5000 - 6000 psi

2. Detektor (1 unit)

a. Lampu : D2 lampb. Rentang panjang gelombang : 190-800 nm atau lebih besarc. Presisi Panjang gelombang : ± 0.1 nm d. Akurasi Panjang gelombang : ± 1 nm e. Lineritas : ≤ 2.5 AUf. Photodiodes : 512

3. Oven Kolom (1 unit)

a. Rentang suhu : 4º C - 65º C or higherb. Presisi suhu : ± 1% atau lebih kecil

4. Autosampler (1 unit)

a. Injection Accuracy : ≤ 2% or better b. Injection Volume range : 0.1 - 100 µl or betterc. Sampel Capacity : min 100 Vial @±1 - 2 ml

5. Manual Injektor & Stand Kit (1 unit)

≤ 0.1% RSD ≤ 1%

6. Degasser (1 unit)

7. Data Processor : Intel Core i3-2100 Processor,min 2GB, Windows 7 Professional,500GBSATA,DVD+RW, Monitor LCD 18,50",Software PDA,Printer

Included of

1. Instalasi2. Pelatihan3. Kolom C18, 5µm,25 cm (min 2 pcs )4. Garansi: minimum 1 tahun5. Tool kits (1 set)6. Buku petunjuk pemakaian asli (plus bahasa indonesia)7. Factory certificate 8. Needle wash (1 set)9. dust cover (1 set)

10. Sampel vial min 1 ml (100 pc/pk)11. HUB W/ Cable12. Stabilizer min. 3 KVA13. Maintenance & autorinsing Kit for pump14. Micro syringe 100µl, min 1 pcs15. compatible mixer min.1 set16. Spare part availability min. 5 year17. Reservoir Tray 1 set

7 SPECTROPHOTOMETER UV-VIS *) Unit 1

Specifications:

a. Spectral bandwitch : ≥ 1 nmb. Wavelength range : 190 - 1100 nm or betterc. Wavelength accuracy : ± 0.2 nm at 656.1 nm or betterd. Stray light : 0.05 % or less (220 nm & 340 nm)

e. Photometric system : Double beam opticf. Photometric range : Absorbance : (-) 2 to 2.0 Abs or higher

Transmitance: 0.0 to 300 % or higherg. Photometric accuracy : ± 0.002 Abs ( at 0.5 Abs ) ; or better

h. Photometer repeatability : ± 0.001 Abs ( at 0.5 Abs)

i. Baseline Stability :j. Baseline flatness : ± 0.0006 abs (200 to 900) or betterk. Light source : W1 and D2 lamp.

l. Noice level :

Included of :

1. Instalation2. Training3. Guarantee min.a year4. Original operation manual : 1 set (plus bahasa indonesia)5. Dust Cover : 1 set6. Tools kit : 1 set7. Glass Filter Holmium Oxide : 1 set8. Shutter Block : 1 set9. Cuvette/cell 1-cm : 1 set

10. Stabilizer 1 KVA : 1unit11. Multi-cell sample compartment unit with 6-cell holder12. Factory Certificate13. Rectangular Quartz Cell, 10 mm : 2 Pcs/set14. Branded personal computer and printer: - Minimum suplied : Intel Pentium Core II Duo or similar

8 DISSOLUTION TESTER *) Unit 11. Specifications :

a. Flask temperatur - Range : 30 to 40 °C or better- Accuracy : ± 0.2 °C or better

b. Bath capacity : 8 flasksc. Spindle speed

- Range : 25 to 250 rpm or better- Accuracy : ± 1 rpm or better

d. Vibration : < 0.1 milse. Voltage : 220 V-240 V, 50/60 Hzf. Supporting systems including with : - 8pcs USP/NF glass dissolution flask

- 8 pcs USP paddles- 8 basket assembly 40 mesh basket- 8 pcs sampling probe

2 Accessories :a. USP / NF Glass dissolution flask : 8 Pcsb. USP all teflon paddle : 8 Pcsc. basket assembly, 40 mesh basket : 8 Pcs

±0.0003 Abs

± 0.00015Abs (at 500 nm)

d. sampling probe : 8 Pcse. USP Calibrator Prednisone Tablet : 30 tabletf. Stainless wire to sink capsule : 1 rolg. Filter : Pack of 100 h. Sinker Japanese Pharmacopoeia Cage : 8 pcs

3 Included of :1. Instalation2. Training3. Dust cover4. Guarantee min. A year5. original operation manual(plus bahasa indonesia)

9 DISSOLUTION TESTER WITH AUTOSAMPLER *) Unit 1Each unit consist of1. Dissolution Tester 2. Autosampler

1. Dissolution TesterSpecifications :a. Flask temperatur

Range : 30 to 40 °C or betterAccuracy : ± 0.5 °C or better

b. Bath capacity : 8 flasksc. Spindle speed

Range : 25 to 250 rpm or betterAccuracy : ± 1 rpm or better

d. Voltage : 220 V-240 V, 50/60 Hze. Supporting systems including with

- 8pcs USP/NF glass dissolution flask- 8 pcs USP paddles- 8 basket assembly 40 mesh basket- 8 pcs sampling probe-USP Calibrator Prednisone (30 tablets)-Stainless wire to sink capsule 1 roll-Filter (pack of 100)

2. AutosamplerSpecifications :

Include precision syringe pump samplingChange and save minimum up to 20 protocols, easy-to-use routines, password protectBuilt-in validation, calibration and diagnostics, plus unique advanced featuresTeflon valves, media replace and system rinse & wash.Stainless probes for media replace and waste; 8 sample system 24 hours sampling time (sustained release)Media replace and system rinse

3 Accessories :a. Validation tool kit : 1 setb. Replacement filters : pack of 100 c. Base-kit, including Device boxd. Sinker Japanese Pharmacopoeia Cage : 8 pcse. Sample and return probe kit : 8 pcsf. E-prob actuator kit : 8 pcsg. Vessel cover : 8 pcsh. Return Harness,6 ft : 8 pcsi. Evaporation caps for 16 mm test tube : pack of 50j. Certified calibrated tachometer : 1 setk. Certified calibrated thermokopel : 1 set

4 Included of :1. Instalation2. Training3. Dust cover4. Guarantee min. 1 year5. Spare Part Availability min. 5 year6. original operation manual (plus bahasa indonesia)7. Alignment kit

10 MIKROSKOP KAMERA *) Unit 1Specifications:

A. Microscope bodya. Lamp : halogen illuminationb. Optical system : UISc. Eyepieces : up to 10xd. Objective : up to 10x, 40x, 100xe. Able to pre -focus & tension adjusment

B. Accessories :a. Compatible Digital Camera : min 12 mpx or better.b.

c. Memory : CompactFlash,microdrive,xD-Picture Card

C. Included of1. Object glass and cover : min 100 pcs2. instalation3. training4. guarantee min. a year5. original operation manual (plus bahasa indonesia)6. dust cover

PC + Sofware for capturing image and recording movie files,can be attached to any desktop or laptop

11 FUME HOOD PROVIDED WITH MICROPROCESSOR CONTROL *) Unit 2Specifications :

a. Exterior dimension (W x D X H in mm) : (1100-1250) x (750-900) x (1100-1600)b. Interior work zone dimension : max. 996 x 672 x 1240

(W x D X H in mm)c. Max. sash opening : 740 mm (vertical rising sash)

d. Exhaust air volume for minimum face velocity : 500 cmh or greatere. Other exhaust outlet diameter : 305 mmf. Light intensity for work surface : > 1000 lux (>100 foot candles) or greaterg. Construction : : Tri wall construction

- Work surface : Phenolic Resin Laminate- Main Body : Fabricated of electro-galvanized steel- Coating : Epoxy polyester hybrid antimicrobial powder coating

h. Controller : MicroprocessorCompleted with airflow alarm kit

Include of :1. Installation2. Guarantee min a year3. Training4. Original operation manual5. Sash stop kit and standar accessories for fume hood (blower, ducting, piping, etc)

12 WATER PURIFICATION SYSTEM *) Unit 1Specifications :

Complete SystemType I : for AAS,GC, HPLCType II : for Micro media, feed water Type I

Type IDaily Volume : min. 10 litreDelivery Flow rate-maximum : Up to 2 L/min

:Organics/TOC : < 5 ppbBacteria (with Biofilter) : < 1 cfu/10 ml or betterEndotoxin (with Biofilter) : < 0.001 EU/mlDNAse (with Biofilter) : < 20 pg/ml or betterRNA (with Biofilter) : <0.002 ng/ml

Type IIDaily Volume : min. 7 litre/hourDaily output : 170 L /day or better

:Organics/TOC : < 20 ppbBacteria : < 1 cfu/10 ml or better

Inorganics ( resitivity @ 25°C) 18.2 MΏ.cm

Inorganics ( resitivity @ 25°C) 10 to > 10 M Ώ - cm

Recirculating : Fully DeionizationSupply voltage : 100 -240 V ac / 50 -60 Hz

Included of :1. Tank 25 L with 5 level switches,2. Deionization Cartrige ( 2 )3. Purification Pack ( 2 )4. Biofilter ( 2 )5. POU filter ( 2 )6. Warranty 1 year7. Install8. Certificate of Conformity

13 DIGITAL BURET Unit 3Spesifikasi:

Dispensing range : 0.01ml - 999.9 mlDisplay : LCDvolume buret : minimal 20 ml

Included :1. safety valve2. microbatteries 2 piece3. telescopic aspiration tube4. bottle adapters minimal 3 piece5. adjustable discharge tube6. recirculation tube7. original operation manual (plus bahasa indonesia)8. instalation9. guarantee min. a year

10. training

14 AUTOMATIC TLC SAMPLER *) Unit 1Specifications:

a. Automatic sample application for qualitative and quantitative b. The system is Controlled by softwarec. Aplication of spots, bands or widerd. External gas supply

Include of1. Dosing syringe starter kit including2. 25 ul Dosing syringe 3. Needle for spray-on application4. Needle for contact application5. Heated spray nozzle6. Sample vial rack for 66 vials 2 ml (12 x 32 mm) 7. Waste bottle 250 ml complete

8. Syringe rinsing tube9. TLC-foil down-holder

10. Compensation ledge for 10 x 10 cm plates or wider11. Screw cap bottle 250 ml, suitable as waste or rinsing bottle12. Gas N2 + Regulator13. Monitor 17" LCD14. Printer 15. Instal16. Training17. original operation Manual (plus bahasa indonesia)18. garansi minimal 1 tahun

15 SEMI AUTOMATIC TLC SAMPLER Unit 1Specifications:

a. System : Semi Automatic , qualitative and quantitative b. Aplication of spotsc. Temperatur range :d. Display : digital or equivalentf. Kapasitas chanel : up to 18 or moreg. Plate dimension : 10x10cm ; 20x20 cm or moreh. Power : 220 V/ 50 hz

Include of1.2.3. Instal4. Training5. original operation Manual (plus bahasa indonesia)6. garansi minimal 1 tahun

16 AUTOMATIC TITRATOR *) Unit 1Spesifications :

a. Application : redox & iodometric titration & Complexometric titration. b. Burette : 10 mlc. operation : Color LCD touch panel or color TFT touch controld. Interface : RS 232e. max. number of sampel : min 100

Accessories :a. Magnetic stirerb. pp beaker set of 500 pcsc. Glass Beaker 80 ml set of 20 pcs d. Burettes for 2 titratione. IQ/OQf. Printer

Included of :

85°C - 90°C or wider

Syringe 50 µl & 100 µl minimal 10 pcsSyringe 25 µl minimal 20 pcs

1. Installation2. Training3. Guarantee min. a year4. Original operational manual (plus bahasa indonesia)

17 ULTRASONIC BATH Unit 2Specifications:

a. Capacity : ± 10 litersb. Temperatur range : up to 70 o C or greaterc. Timer : minimum 1-30 minutes or greaterd. Frequency : minimum 35 KHze. Display : LEDf. Material : Stainless steel or equivalent

Include of1. Guarante min. A year2. Original operation manual (plus bahasa indonesia)3. Dust cover ( 1 unit)4. Tray/basket (stainless steel) (1 unit)5. Hinged Lids made of Stainless Steel to be mounted on the unit

18 SEPARATORY FUNNEL SHAKER Unit 2Specifications :

a. Shaking method : Vertical and rotary shakingb. Shaking speed : Approx 20 to 210 rpm or greaterc. Speed control : Stepless speed control by thyristord. Timer : 0-60 min or more e. Motor : DC motor, 90 Wf. Voltage : 100/220V, single phase, 2.2/1.0 A /50/60 Hz

Accesories a. Separatory funnel holder : 1 set (6 funnel) for 100 - 1000 mlb. Fuse : 1 pcsc. Carbon Brush : 1 set

Included of1. Training2. Guarantee min.1 year3. Manual book4. Certificate of Origin

19 SHAKER Unit 3Specifications :

a. Motion : Orbital b. Speed

: 0 to 200 or greaterc. Load capacity : minimal 8 kg

- Speed frequency (min-1)

d. Amplitude (mm) : min 10 mme. Power : 115-230 V, 50/60 HZf. Timer : Up to 60 min or greaterg. Dimention : min (380 W x510 D x 120 H) mmh. Shaking platform : minimal 300 x 300 mm

Acessories :1. fastening bar 545 mm lengthways minimal 2 unit2. clamping rolls 375 mm, widhtways minimal 3 unit3. clamping rolls 375 mm, widhtways minimal 3 unit

Included of1. Instalation2. Guarantee min. 1 year3. Spare part availability min. 5 year4. Original operation manual

20 SEMIMICRO BALANCE *) Unit 4Specifications :

a. Capacity max : minimal 200 g or moreb. Readability : 0.01 mgc. Reapeatability : ± 0,07 mg d. Liniearity : ± 0,2 mg or bettere. Power : 100 - 240 V, 50-60 Hzf. Display : LCD or better

Acessories :1. Printer2. Antistatic brush3. Stainless steel spatulla, 6 x 180 mm @ 5 pcs4. Stabilizer 500 mA5. External Calibrator 100 and 200 g E2

Included of :1. Installation2. Training3. Original operational manual book4. Guarantee min 1 year5. Spare part availability min 1 year6. Dust cover

21 CENTRIFUGE Unit 2Specifications :

a. Max Speed (rpm) : minimal 4000 rpm swing / minimal 4000 rpm angleb. Max capacity : up to 4 x 200 ml (swing out)c. Control system : Microprocessor

d. Display digital fuse number speed time : yese. Timer : 1-99 minutesf. Power requirements : 220-240; 50-60 Hz

Accessories :1. : 6 x 50 ml

Include of :1. Guarantee min. 1 year2. Original operation manual (plus Bahasa indonesia)3. Spare part availability min. 5 year

22 pH Meter Berdiri *) Unit 5Specifications :

a. pH Range : 0.00 to 14.00 or widerb. pH Relative accuracy : ± 0.005 or lessc. pH Resolution : variable 0.001 / 0.01/0.1d. mV Range : -1800 to 1800 or widere. mV Relative Accuracy : ± 0.2 or lessf. mV Resolution : 0.1 or betterg. Temperature range : 0 to 100 0C or widerh. Acessories : Technical buffer pH = 3 point : acid, neutral, base @ min 250 ml

Power adapterStabilizer

Included of :1 Installation2 Training3 Guarantee min 1 year4 Original Operation manual (plus bahasa indonesia)5 Storage solution6 spare part availability 5 year7 electrolyte solution

23 ALAT HIDROLISIS LEMAK Unit 1Specifications :

a. Max. Temperature : min 350 °Cb. Capacity perbatch : 6 samplec. Power : 230 V, 50/60Hz

Consist of:1 The crude fibre digestion unit (1)2 Plus a filter with 6 funnels3 Soft polyethylene bottle with a narrow mouth (1000 ml) : 14 100 folded filter as well as 250 gr boiling stones5 Glass condensers6 Ring shaped rest

- Angle- Rotor

7 Silicone rubber tubings8 Beakers (1000 ml)9 Power cord

10 Standard Accessories

24 HEATING MANTLE Unit 4Specifications :

a. Flask capacity : 500 mlb. Temperature range :c. Temperature controller : digitald. Material : Nichrome Wire or equivalent

Include of :1 Guarantee min. 1 year2 Spare part availability min. 5 year3 Original operation manual (plus bahasa indonesia)

25 ANALYTICAL BALANCE with PRINTER *) Unit 6Specifications :

a. Capacity max : min 220 g b. Readability : 0,0001 gc. Reapeatability : ≤ 0,1 mgd. Liniearity : 0,2 mg or less

Included of:1 Printer2 Instalation3 Dust cover4 Guarantee min 1 year5 Spare part availability min 5 year6 Original operational manual book7 Weight Standart Class E2 Capacity 100 & 200 g

26 APPARATUS FOR DETERMINATIONS OF SAPONIFICATIONS NUMBER AND OH NUMBER Unit 1Specifications :

a. Digestion Unit : 1b. Sample positions : 6 samplesc. Dimension (W x H x T) : approx 31 x 90 x 38 cmd. Weight : approx 28 kge. Nominal Voltage of the heating block : 220 - 240 VAC 50/60 Hzf. Power Consumptions : approx 800 Watts

Included of :1 Instalation2 Training3 Heating block

up to 4500C

4 Controller5 Glassware ( 6 pcs)6 Straight Air Condenser

27 AUTOMATIC SERIAL SOXHLET SYSTEM *) Unit 1Completed with WaterbathEach set consist of :1 Extraction system : 1 unit2. Chiller : 1 unit

1. Extraction systemConsists of Extraction Unit and Control UnitSpecifications :

a. Solvent volume : minimum 100 mlb. Capacity per-batch : 6 samplesc. Power supply of control unit : 230V/50/60 Hz

Accessories 1. Extraction beaker glass : 12 pcs2. Holder for extraction Thimbles : 6 pcs3. Extraction thimbles 33 x 80 mm : 25 pcs4. Pair of glass tongs 5. Box of boiling stones 250 g6. Holder for 6 extraction beaker7. Tubing set complete8. Line filter 16 A (just for 230V)

2. Chiller Specifications :

a. temperature range : 0 °C to 100 °Cb. temperature ontrol :c. Footprint : 210 x 400 x 500 mm or greater

3. Includes of1. Instalation2. Training3. Dust cover4. Guarantee min 1 year5. Spare part availability min 5 year6. Original operation manual

28 GLASS WARE DRYER Unit 3Specifications :

a. Dimensions ( W x D x H ) : Approx 380 x 380 x 1150 mm b. Nominal Voltage : 230 V ac / 50 Hz / 1000 Watt max.c. Number of drying tubes : min. 56 tubes, consist of small & large vesselsd. Temperature in drying vessels : up to 50°C

± 0.2 °C

e. Electric timer : Approx 120 minutes

29 ROTARY VACUUM EVAPORATOR + CHILLER *) Unit 1Consist of :A. Rotary Evaporator (1 unit)

Specifications : a. Operating Voltage : 100-240V, 50-60 Hzb. Rotation Speed : 20 to 280 rpm or greater

B. Heating Bath (1 unit)a. Temperature Range :b. Bath Capacity : 4 L or greater

C. Vacuum Pump with controller (1 unit) a. Capacity :b. Final vacuum (absolute) : ≤ 10 mbar

D Recirculating Chiller (1 unit)a Temperature range : Fix or adjustableb Power Supply : 220/240 V, AC, 50/60 Hzc Footprint of chiller : 210 x 400 x 500 mm or greater

E Acessories : :1. vapor temperature sensor2. condenser3. Manual vacuum controller4. Woulff botle minimal 1 pcs

F Included of :1 Installation2 Training3 Guarantee min 1 year4 Original Operation Manual (plus bahasa indonesia)

30 MICROWAVE DIGESTION Unit 1Spesification :a. Temperature control : every vesselb. Jumlah vessel : min 10 vesselc. Volume vessel : min 10 mld. Vessel material : PTFE/Teflone. Temperature :f. Presisi temperatur : ± 1°C at 200°C

Accessories :1. Turntable with 10 pcs of Vessels 60 mL

- Forming Systems for Vessels 60 mL

20 to 150 0C or wider

1.5 m3/h or greater

50-2600 C

- Consumables for Vessels 60 mL For approx 2500 digestions- Spare of Vessels 60 mL , pk/10

2. Turntable with 24 pcs Quartz Vessels 10 mL- Forming Tools for Quartz Vessels- Rupture Disc for Quarts Vessels, pk/5- Lids for Quartz Vessels, pk/4- Fixations Plate of Rupture Disc - Spare of Quartz Vessels 10 mL, pk/24

Included of1. instalation2. training3. guarantee min. a year4. original operation manual (plus bahasa indonesia)

31 WATERBATH Unit 2Spesifications

a. Capacity : ± 14 literb. Casing, container and cover made of : Stainless steelc. Temperature range : 10 - 95 C or better⁰d. Resolution :h. Display : LEDi. Timer : 1 minutes to 99 hours or better

Accessoriesa. Flat stainless steel cover with openings& concentric ring sets : 6 holesb. Gable stainless steel cover mounted for condensate drainagec. water level control

Included of :1. Instalation2. Guarantee min. a year3. Original operation manual (plus bahasa indonesia)

32 SPIRAL SERIAL FLASK HEATER Unit 1Spesifikasi:

a. Temperature :b. Voltage : 230 Vc. Frequency : 50 - 60 Hzd. Wattage : 1800 We. For Flask : 50 - 250 mlf. Heating place : Minimal 6g. Dimensi : Min 590 x 220 x 125 or widerh. Weight : Min 7 kg

Included of1. original operation Manual (plus bahasa indonesia)

± 0.1 C⁰

up to 425°C

2. instalasi3. garansi min 1 tahun

33 UPS Unit 4Spesification :

a. Capacity : min 10 Kvab. Input voltage : 220 volt (min 1 fase)c. Input Frequency : 45 - 65 Hzd. Output Voltage : 220 volt (min 1 fase)e. Output Frequency : 50 / 60 Hzf. Overload : up to 120% 0r betterg. Runtime : approx 10 minutes

Included of :1. Installation2. garansi spare parts,bateray & service minimal a year3. Original operation manual (plus bahasa indonesia)

34 UV CABINET Unit 1Specifications :

a. UV lamps in combination with UV viewing boxb. Consist of : UV lamps for 2 wavelength 254/365nm (2 x 6W) or better

Included of :1. Guarantee min. 1 year2. Spare part availability min. 5 year3. Original operation manual (plus bahasa indonesia)

35 HOT PLATE STIRRER Unit 1Specifications :

a. Heating plate material : glass Ceramic or equivalentb. Stirring capacity with H O ₂ : minimal 4 L c. Speed range : up to 1100 rpm or higherd. Temperature range : up to 550 °C e. Power Supply : 220-240 V / 50-60 Hz

Included of :1. Magnetic bar minimal 5 pcs2. Guarantee min.a year3. Original operation manual (plus bahasa indonesia)

36 HOT PLATE SEDANG Unit 2Specifications :

a. Min Temperature : 5°C b. Max Temperature : 400°C ± 10 %c. Heating Surface (width x Depth) : approx 26.0 x 26.0 cmd. Power : 220 - 240 V / 6.40 A / 1530 W

e. Type : Ceramic Top Hot Plate or equivalentf. Approvals : CSA, CE, NRTLc (CCSAUS)g. Overall dimensions (W x H x D) : 28,7 x 41.1 x 10.1 cm ± 10 %

37 HOT PLATE BESAR Unit 1Specifications :

a. Digital LCD displayb. Anodizedc.d. Plate size(mm) approx 300 x 600e.f. Operating manual prosedur (English and Indonesian)g. Min guarantee 1 yearh. Power supply 230 V/ 50-60 Hzi. Dimensions at least 650 x 350 x 140

38 MUFFLE FURNACE Unit 2Specifications :

a. Capacity : 14 L ± 10 %b. Temperature range : up to 1100°Cc. Interior Dim ( W x D x H) : 325 x 254 x 172 mm ± 10 %d. Exterior Dim ( W x D x H) : 485 x 510 x 533 mm ± 10 %e. Temp Uniformity : ±2.2°C at 1000°Cf. Temp Stability : ±0.3°C at 1000°Cg. Power Output : max. 3100 Wattsh. Voltage : 12.9A / 208-240Vi. Temperature control : Digital single set point control

Includes:1. Warranty : min 24 month2. Supplied with cord and plug set

39 DISTILLATION UNIT*) Unit 1Specifications :

a. Kjeldahl nitrogen and protein distillation unit; Alcohol& phenol distillation; volatil acids & Cyanide determinations

b. Acid-resistant pumpc. Safety features:

- Protective door- Sample tube detector- Service door sensor- Drip tray- Cooling water flow sensor

d. Methods can be store : 9 or moree. Tube volume : min 250 ml (1 set)f. Recovery rate : min. 99.5%g. Reproducibility (RSD) : minimum ± 1%

Temperature range up to ± 300°C

Accuracy max 0.3°C

h. Detection limit : minimum 0,1 mgi. Voltage : 220 V - 240 V, 50/60 Hzj. Steam Performance : 160 ml / 5 min or betterk. Power consumption : 2200 W or lessl. Set of storage tank : 2 x 20 liter

40 DISTILLATION UNIT FOR ANALISYS SO2 WITH TITRATOR *) Unit 1Specifications :A. Destillation Unit

a. Aplikasi determination of sulfur dioxida.Can be used for Kjeldahl nitrogen and protein distillation unit; Alcohol& phenol distillation; volatil acids & Cyanide determinations ,

b. Acid-resistant pumpc Safety features:

- Protective door- Sample tube detector- Service door sensor- Drip tray- Cooling water flow sensor

d Methods can be store 50 or moree Tube volume : min 250 ml (1 set)f. Recovery rate : min. 99.5%g. Reproducibility (RSD) :h. Detection limit : minimum 0,1 mgi. Voltage : 220 V - 240 V, 50/60 Hzj.. Power consumption : 2200 W or lessk. Set of storage tank : 4 x 20 liter

B. Titratora. End point titration automaticb. Display of consumtion of titration solutionc. Display of pH value

41 VACCUM PUMP with GLASS FILTER HOLDER Unit 1Each set consist of1. Pump : 1 unit2. Glass Filter Holder : 1 unitPumpSpecifications :

a. Presssure : ≥ 4 Barb. Power Supply : 220-240 V/ 50-60 Hz

Glass Filter HolderSpecifications :

a. Diameter : 47 mmb. Joint connection : Compatible

≤ ± 1%

c. Funnel and flask : Glass d. Flask capacity : 1000 mle. stopperf. clampg. fritted base

Accessories1.2. Support Screen

42 MIKRO BALANCES PROVIDE WITH ANTIVIBRATION TABLE *) Unit 1Specifications :

a. Weighing capacity : 6 - 7 gb. Readability : 0,001 mgc. Repeatability : ≤ 0,001 mg or betterd. Linearity : ≤ 0,004 mg or bettere. Display : touchscreen

Accessories1. Antivibration Table (1 set) 2. External calibrator 2 and 5 gram E2 (1 set)3. Printer (1 unit)4. Filter weighing kit (1 set)5. Antistatic brush (1 set)6. Dust Cover (1 set) 7. Stabilizer 500 VA (1 unit)

Include of :1. Instalation2. Original operational manual book (plus bahasa indonesia)3. Guarantee min 1 year4. Training

43 THERMOHYGROMETER DIGITAL Unit 7Specifications :

a. Temperature Range : (-5)o to 55 oC or widerb. Humidity Range : 20 -95 % or widerc. Temperature Accuracy :d. Humidity Accuracy : ±5 % rF (rh)

Accessories :1. Battery compatible @ 2 pcs2. With clock3. accesory for wall mounting (screws or adhesive tape).

Include of :

Silicon rubber laboratory tubing, compatible,L= 2 mɸ

±1˚C

1. Original Operation Manual (plus bahasa indonesia)2. Guarantee min 6 months

44 STOP WATCH DIGITAL Unit 2Specifications :

a. 7 digitsb. Measurable ± 10 hours c. Net time, split time, 1st/2nd place times

Included of :1. Guarantee min. 1 year2. original operation manual (plus bahasa indonesia)

45 VACUUM FILTER HOLDER Unit 2Spesifications

a. autoclavable

b. polysulfone or polycarbonate

c. upper capacity at least 250 ml

d. lower capasity at least 250 ml

e. connection for vacuum pump

f. With steril membrane 0.45

g. Membrane diameter 47mm

46 ULTRASONIC PIPPET WASHERS Unit 1Spesifications

a. dimension at least 300 x 400 x 800mmb. capacity at least 12 L for:

1 ml = 250 pcs5 ml = 200 pcs10 ml = 130 pcs

c. temperatur water for cleaning solution up to 40degC with detergentd. vertical tanke. stainless steel cabinet

Include of1. Basket (mm) at least 146 (dia) x 550 (H)2 Min guarantee 1 year3. Trainning4. Standar operating procedur (English and Indonesia)5. Power supply 100V/ 50-60 hz6 Step up & down transformer7 Rack8 Manual Pump9 Water supply hose

10 Drain hose

11 Detergent

47 AUTOMATIC COLONY COUNTING AND ANTIBIOTIC ZONE READER Unit 1Spesifications

a. Integral PC (all in one PC), CCD camera, and lensb. USB integral camera, Min camera 1.4m pixelc. Light shield 2 sliding doors to prevent excessive ambient lightd. Complies with 21 CFR part 11, GLP e. With software and Database store all dataf. Detection: fully automatic with manual over rideg. Count Modes : Separation of touching colonies, colur mode, shape mode, SRD zone sizing and statistical analysish. Used standard up to 150 mm petri dish i. Smallest detectable colony at least 45 micronj. Inc mouse and keyboard, IQ/OQ documenting.k. Trainningl. Min guarantee 1 year

m. Standar operating prosedur (English and indonesian)

48 Unit 1Spesifications

a. made from glass with mercuryb.c. min guarantee 1 yeard. standar operating procedure

49 VORTEX MIXER Unit 2Spesifications

a. speed range up to 3000 rpmb. used for at least 1.5 ml test tubec. with analog display and control scaled. orbit diameter 4 mme. speed control with knob for different modes of mixingf. power supply 230 V/ 50-60 hzg. min guarantee 1 yearh. standar operating procedure (English and Indonesia)

50 MINI PERSONAL CENTRIFUGE Unit 1Spesifications

a. Max speed : at least 12.000 rpmb. Max rcf : at least 14.000 rpmc. Rotor capacity : at least 12 x 1.5/2 ml test tubed. Noise level : < 56 dB(A)e. Timer : up to 99 minf. Power supply : 230 V/50-60 Hzg. Power requerement : up to 100 W

THERMOMETER AIR RAKSA SUHU -38 S/D 50°C

temperature range -38 s.d 50°C

h. Min guarantee 1 yeari. Operating manual procedur (English and Indonesia)j. Inc with rotor

51 SEROLOGI PIPET Unit 4Spesifications

a. baterry rechargeableb. autoclavablec. used for pipette types 1 ml to 100 mld. at least 7 hours operatione. aspirating speed at least 25 ml in 5 secondf. membrane filter replaceableg. touch control adjustable speed

Include 1. holder2. Min guarantee 1 year3. Standar operating procedur (English and indonesia)4. Comfortable design for operation

52 DIGITAL THERMOMETER WITH SENSOR Unit 2Temperatur range

-250degC to 400degC : 1 unit

Spesificationsinc: impact absorbing holster and two 80PK-1 beaded probe thermocouples

a. temperatur range for -250degC to 400degC: b. Batteries 3 AAc. Accuracy above -100degC: ± 0.05% + 0.3degC d. Min guarantee 1 yeare. Standar operating procedure (English and Indonesia)

53 Unit 1Spesifications

a. made from glass with mercuryb.c. min guarantee 1 yeard. standar operating procedure

54 CABINET STERILIZING Unit 1Spesifications

a. with UV lampb. dimensions : at least 860 x 490 x 1750 mmc. accesoriesd. interior decoration of a stainless steele. include fall prevention metal fittings

THERMOMETER AIR RAKSA SUHU -10 S/D 50°C

temperature range -10 s.d 50 °C

f. Power supply AC 100 V 50/60 Hzg. Step up & down transformerh. with timer and analog display for clocki. number of sheet up to 10j. 2 doorsk. min guarantee 1 yearl. Operating manual prosedur (english and Indonesian)m Trainning

55 TURBIDIMETRI Unit 1Spesifications

a. for measurement 0.0 up to 4.0 McFarlandb. automated digital display when usedc. data store softwared. used flexible pipette from glass or plastic 12mm x 75 mme. verification meansurement with standart kit

Included of1. Instrument kit

- 4 baterai AAA alkaline- instrument user manual CD- product certified- RoHS certified

2. Standart kit- scale 0 McF, 0.5 McF, 2.0 McF, 3.0 McF

3. Min guarantee 1 year4. Trainning5. Standart operation procedur (English and indonesia)

56 SAFETY CABINET CLASS II *) Unit 1Spesifications

a. external dimensions max 1500 x 900 x 1700 mmb. HEPA filter with 99,999% efficiency at 0.1 to 0.3 microns or betterc. microprossesor control systemd. with UV lamp, support stande. dual fan systemf. stainless steel main body with stainless steel armrestg. epoxy coatedh. sound emision < 60 dBAi. downflow velocity 0.3 ± 0.1 m/sj. Inflow avarege 0.4 ± 0.1 m/s

Included of1. Min guarantee 1 year2. Trainning3. Standar operation procedure (English and Indonesia)

57 COOLED INCUBATOR Unit 2Spesifications

a. Volume : 250±10 literb. Temperatur range : -12˚C to 60˚C±5˚Cc. Interior chamber : Stainless steeld. Over-and undertemperatur protecting with alarme. Digital displayf. Works calibration certificateg. interior dan exterior doorh. Fan Speed adjustable from 10 - 100 % in 100 % stepsi. electtronic microprossesor temperatur controllorj. temperatur setup: 3 point calibration on controllor k. electrical supply 230 V/ 50-60 Hz

Included of1. Min guarantee 1 year2. Trainning3. Operating manual prosedur (english and indonesian)

58 PCR HOOD Unit 1Spesifications

a. with UV lamp and timerb. HEPA filter H14 or >99.99% at 0.1 to 0.3 micronsc. Internal dimension at least 900 (max 1200) x 500 x 500 mmd. external dimensions max 1200 x 700 x 1000 mme. epoxy powder coated with antimicrobial coatingf. pre filter g. air flow velocity average 0.3 m/s (60 fpm)h. power supply 220-230 v/ 50-60 hz

Included of1. Min guarantee 1 year2. Trainning3. Operating manual prosedur (english and indonesian)

59 STERITEST PUMP *) Unit 1Spesifications

a. pump with bottle holder and canister holderb. autoclavablec. power supply 230 V/ 50-60 Hzd. Materials of Construction

Frame, pump head, Pump headcover, and bottle holderkeypad and screen window:Drain TrayDrain Tray tubing

e. Dimensions : pump: approx. 35 x 30 x 25 cm: pump with bottle handle, canister handle approx. 59 x 34 x 53 cm

f. compatible with canister for sterility test

Included of1. consumable and accesories

Ampoule breaker for sterility test (1 pk)Canister with membran for sterility test for liquids in ampoules or collapsible bags (2 pk)Canister with membran for sterility test for antibiotic, 10 / pack (2 pk)Canister with membran for sterility test for solvent, creams, ointment (2pk) veterinary injectables, 10 / pack

2. Min guarantee 1 year3. Trainning4. Standar operation operation (English and Indonesia)

60 INKUBATOR KERING Unit 1Spesifications

a. compatible with thermo block for at least 20 x 1.5ml, 20 x 2 ml, 8 x 15ml, 4 x 50ml test tubesb. temperatur setting at least 4degC to 99degCc. temperatur range at least 30degC to 99degC d. digital timer at least 1 min to 90 hourse. incubating accuracy at least ± 1.0degCf. heating speed at least 5degC/ming. cooling speed at least 5degC/min between 99degC and 25degCh. power supply 220-230V/50-60 hz

Included of1. min guarantee 1 year2. trainning3. standar operation precedur (English and Indonesia)

Assesories1. thermoblock for test tube 1,5ml2. thermoblock for test tube 2ml3. thermoblock for test tube 15ml4. thermoblock for test tube 50ml5. box for all tube types, interior dimensions at least 13 x 8 x 5 cm

61 MICROCENTRIFUGE Unit 1Spesifications

a. speed : min 16,000 rpmb. max capacity : at least 24 x 1.5/2ml, 6 x 50ml mlc. timer : up to min 60sd. dimension : max 40 x 70 x 35 cme. refrigerated : at least -10degC to 40degCf. temperatur control

g. power supply 230 v/ 50-60 Hzh. LCD display

Included of1. rotor for 1.5/2ml, 15ml, 50ml, microplate rotor2. Trainning3. Min guarantee 1 year4. Operating manual prosedur (English and Indonesia)

62 PIPET MIKRO DAN ACCESSORIESukuran

Unit 1Unit 2Unit 1Unit 1

0,5 - 5 ml Unit 11 - 10 ml Unit 1Stands pipette Unit 1

Spesificationsa. autoclavableb. daya tahan yang tinggi terhadap asam, basa, dan tumbuhnya jamurc. stans pipette at least for 6 pipetd. adjustment display to a spesific volumee. min guarantee 1 year

0,5 - 10 µl10 - 100 µl100 - 1000 µl1000 µl