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7/18/2019 praktikum 1 biokimia
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LAPORAN PRAKTIKUM
BIOKIMIA
NAMA
NIM
KEL.PRAKTIKUM/KELAS
JUDUL
:
:
:
:
MUHAMMAD RIZKY
08121006024
B/GENAP
DETEKSI GULA PEREDUKSI
LABORATORIUM BIOKIMIA
JURUSAN FARMASI
FAKULTAS MATEMATIKA DAN ILMU
PENGETAHUAN ALAM
UNIERSITAS SRI!IJAYA
201"
LAPORAN PRAKTIKUM
7/18/2019 praktikum 1 biokimia
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BIOKIMIA 1
NOMOR PRAKTIKUM : I
NAMA PRAKTIKAN : MUHAMMAD RIZKY
NIM : 08121006024
JUDUL PRAKTIKUM : DETEKSI GULA PEREDUKSI
TUJUAN :
Mahass!a "a"#$ "%"aha" #&'s# (%)%*s +$,a #%&%($*s s%-a&a $"$"
.a'+ "%&$#a*a' *%)%&a"#,a' (asa& (a,a" /(a'+ *%ah,a' /*"a *,'*
PRINSIP KERJA :
M%,a*$*a' #&'s# (%)%*s +$,a #%&%($*s (%'+a' &%a*s %h,'+ (a' /%'%(-)
DASAR TEORI
Ka&/h(&a) "%&$#a*a' s%'.a!a .a'+ )%&/%')$* (a& ",%*$, *a&/'
h(&+%' (a' *s+%'3 Ka&/h(&a) a(a,ah #,sa*a&(a "%&$#a*a' s$"/%& %'%&+
$)a"a #a(a "a*a'a'3 Nas *%)%,a a+$'+ a(a,ah /%/%&a#a -')h "a*a'a'
"%'+a'($'+ *a&/h(&a)3
Ka&/h(&a) a(a,ah s%'.a!a .a'+ "%'+a'($'+ $'s$&5$'s$&: H (a' O
)%&$)a"a )%&(a#a) ((a,a" )$"/$h5)$"/$ha' .a)$ *&a5*&a 793 D'a"a*a'
*a&/h(&a) *a&%'a s%'.a!a5s%'.a!a ' s%/a+a h(&a) (a& *a&/' (a,a" s%'.a!a
)%&s%/$) #%&/a'('+a' a')a&a H (a' O s%&'+ 2 /%&/a'('+ 1 s%#%&) a&3
Ja(6H12O6 (a#a) ()$,s 6;H2O<6 12H22O11 s%/a+a 12;H2O<11 (a'
s%)%&$s'.a (a' #%&$"$sa' %"#&s ()$,s s%/a+a 'H2'O' a)a$ ' ;H2O<'3
Ka&/h(&a) (/a+ "%'a( /%/%&a#a *,as a)a$ +,'+a' s%s$a (%'+a' sa)
sa)'.a )%&ha(a# =a)5=a) #%'+h(&,ss3 Ka&/h(&a) a)a$ +$,a (/a+ "%'a( %"#a)
*%,as #**:
13 G$,a .a'+ s%(%&ha'a a)a$ "'sa*a&(a *%/a'.a*a' a(a,ah s%'.a!a5
s%'.a!a .a'+ "%'+a'($'+ ,"a (a' %'a" a)" *a&/'3 Ka&/h(&a) .a'+
"%'+a'($'+ 6 *a&/' (s%/$) h%*ssa3 G$,a .a'+ "%'+a'($'+ *a&/'
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(s%/$) #%')sa3 K%/a'.a*a' +$,a s%(%&ha'a a(a,ah "%&$#a*a' #,h(&*s
a,(%h(a .a'+ (s%/$) a,(sa (a' #,h(&*s *%)' (s%/$) *%)sa3
23 O,+sa*a&(a s%'.a!a /%&s ($a a)a$ ,%/h +$,a s%(%&ha'a .a'+
(h$/$'+*a' ,%h #%"/%')$*a' as%)a, a')a&a +$+$s a,(%h(a (a' +$+$s *%)'(%'+a' +$+$s h(&*s,3 B,a ($a +$,a (+a/$'+*a' (#%&,%h (sa*a&(a /,a
)+a (#%&,%h )&sa*a&(a (a' s%%&$s'.a *a)a' #%'++a/$'+a' /%&sa"a5sa"a
+$,a ' (s%/$) *a)a' +,*s(a3
>3
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(h$/$'+*a' (a,a" *a)a' +,*s(a3 P,sa*a&(a "%,#$) #a) s%,,$,sa (a'
(%*s)&'33
63 G,*s(a (/%(a*a' (a& ,+ (a' #,sa*a&(a .a)$ ,%h *%'.a)aa' /ah!a
"%&%*a "%'+a'($'+ ",%*$, /$*a' +$,a .a'+ (h$/$'+*a' (%'+a' +$,a ,%h
*a)a' +,*s(a
G$,a &%($*s a(a,ah s%"$a +$,a .a'+ "%",* *%"a"#$a' $')$* "%&%($*s
(*a&%'a*a' a(a'.a +$+$s a,(%h( a)a$ *%)' /%/as3 A,(%h( (a#a) )%&*s(as
,a'+s$'+ "%,a,$ &%a*s &%(*s3 Na"$' +$+$s *%)' )(a* (a#a) )%&*s(as s%-a&a
,a'+s$'+ +$+$s *%)' )%)a# ha&$s ($/ah "%'a( a,(%h( (%'+a' #%&#'(aha'
)a$)"%&* .a'+ "%"'(ah*a' +$+$s *a&/', *% /a+a' a*h& &a')a3 M'sa*a&(a
.a'+ )%&"as$* +$,a &%($*s a')a&a ,a' +,$*sa &$*)sa +,s%&a,(%h(a (a'
+a,a*)sa3 U')$* (sa*a&(a -')h'.a a(a,ah ,a*)sa (a' "a,)sa3 S%(a'+*a' .a'+
)%&"as$* +$,a ''5&%($*s a(a,ah s$*&sa3 G$,a ''5&%($*s (-&*a' (%'+a' )(a*
a(a'.a s)&$*)$& &a')a )%&/$*a s%h'++a )(a* &%')a' )%&ha(a# #&s%s *s(as
&%($*s3 Pa(a #,"%& +,$*sa s%#%&) a",$" (a' )$&$'a' a",$" ;"a,)(%?)&' (a'
(%?)&'< "a*&",%*$,'.a ("$,a (%'+a' +$,a &%($*s3 U"$"'.a +$,a #%&%($*s
.a'+ (has,*a' /%&h$/$'+a' %&a) (%'+a' a*))as %'=" ("a'a s%"a*' )'++
a*))as %'=" "a*a s%"a*' )'++ #$,a +$,a #%&%($*s .a'+ (has,*a'3 P%&s%')as%
+$,a &%($*s ( (a,a" )$&$'a' a",$"@#a) (s%/$) (%'+a' (%?)&s% %$a,%')
G,$*sa
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G,$*sa "%&$#a*a' sa,ah sa)$ )#% "'sa*a&(a (%'+a' &$"$s ",%*$,
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#as& ;s$*&sa< )%)a# ',a *a,&'.a sa"aC3 M%&$#a*a' +$,a .a'+ /a'.a*
()%"$*a' ( a,a" )%&$)a"a (a,a" /$ah a'++$& ;*a&%'a'.a (s%/$) +$,a a'++$&<3
G,$*sa $+a (s%/$) (%*s)&sa *a&%'a s)&$*)$&'.a s%/a+a' /%sa& /%&a(a (a,a"
/%')$* D5 .a*' D5+,$*sa3
G,$*sa "%&$#a*a' "'"%& .a'+ ()%"$*a' ( a,a" s%/a+a ("%&
sa"#a #,"%&3 Ka&/h(&a) .a'+ (*'s$"s )$/$h $"$"'.a ($/ah "%'a(
+,$*sa (a' "%'+a,a" s&*$,as (a,a" )$/$h ;(a,a" (a&ah "%'+a'($'+ 0089
s%(a'+*a' (a,a" $&'% 029 +,$*sa<3 Da,a" #%&(a+a'+a' +,$*sa (/$a) (a&
h(&,sa a",$" G,$*sa /asa'.a )%&s%(a (a,a" /%')$* =a) /%&!a&'a #$)h a)a$*&s)a, #a(a)3 G,$*sa (#&($*s s%-a&a *"%&s, "%,a,$ h(&,ss #a) (%'+a'
/a')$a' %'="3 G,$*sa (a#a) ( &%($*s "%'a( s&/), .a'+ /asa'.a (*%'a,
s%/a+a +,$s),3 D"a'a +$+$s a,(%h(a #a(a +,$*sa (+a') "%'a( +$+$s
a,*h, (%'+a' a(a'.a #%'a"/aha' +as h(&+%'
G,$*sa "$(ah )%&*s(as ,%h #%&a* a)a$ ' )%"/a+a3 B,a
()a"/ah*a' (%'+a' ,a&$)a' #%&a* ')&a) a"'a* a*a' )%&a( -%&"' #%&a*3
M%&%($*s ,a&$)a'5,a&$)a' %h,'+ .a)$ (%'+a' )"/$,'.a %'(a#a' *$#& *s(a
La&$)a' %h,'+ )%&(& a)as ,a&$)a' %h,'+ A: ,a&$)a' $SO4 (a' ,a&$)a' %h,'+
B: ,a&$)a' (a& Na5K5)a&)&a)
ALAT DAN BAHAN
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LALU MASING MASING LARUTAN DIPANASKAN SELAMA 20 DETIKLALU DITAMBAHKAN DENGAN NaOH 2 TETES SEBAGAI INDIKATORSEHINGGA BERUBAH FARNA3 HITUNG DENGAN STOPFATH LAMA
PERUBAHAN YANG TERJADI SEMENJAK DITAMBAHKAN NaOH
BUAT AMPURAN GLUKOSA LARUTAN BENEDIT DENGAN AIRDENGAN KONSENRASI PERBANDINGAN BENEDIT DAN AIR DENGAN
KONSENTRASI 1:1 1:2 1:> 1:4
SIAPKAN ALAT DAN BAHAN
A,a) :
Ta/$'+ &%a*s
Ra* )a/$'+ &%a*s
G%,as $*$&
P%'a'+as a&
S#!a)-h
Baha' : La&$)a' B%'%(-)
La&$)a' %h,'+ A (a' B
La&$)a' +,$*sa
La&$)a' A$a(%s))
ARA KERJA UJI BENEDIT
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MASING MASING LARUTAN DIPANASKAN HITUNG LAMATERJADINYA PERUBAHAN FARNA DENGAN STOPFATH
BUAT GLUKOSA DENGAN KONSENTRASI MENAMPRKAN HLING ADAN B MASING 10 TETES DENGAN MEMASUKKAN EHLING A
TERLEBIH DAHULU BARU EHLING B SETELAH ITU TAMBAHKANDENGAN AIR DENGAN KONSENTRAS EHLING DAN AIR 1:1 1:2 1:> 1:4
SIAPKAN ALAT DAN BAHAN
ARA KERJA UJI ELING
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DATA HASIL PENGAMATAN
13 D%'+a' &%a+%' B%'%(-)
KONSENTRAS
I
DITAMBAH
BENEDIT
HASIL DENGAN
PEMANASAN
SELAMA 20
DETIK
SETETES NaOH
FAKTU
N&"a, @ &a'#a
a&
B%&!a&'a a+a* /&$ B%&!a&'a -*,a) 7 (%)*
1 : 1 +,$*sa
(a' a&
B%&!a&'a a+a* /&$ *,a) -%'(%&$'+
'++a
17 (%)*
1:2 +,$*sa (a'
a&
B%&!a&'a a+a* /&$ B%&!a&'a -*,a)
"$(a
24 (%)*
1: 4 +,$*sa (a'
a&
B%&!a&'a a+a* /&$ B%&!a&'a '++a
-%'(%&$'+ &a'+%
4 (%)*
23 D%'+a' &%a+%' %h,'+ ; %h,'+ A B <
K's%')&as DITAMBAH
EHLING A
DITAMBAH
EHLING BDIDIAMKAN
SELAMA 1
MENIT
HASIL DENGAN
PEMANASANSELAMA 20
DETIK
SETETES NaOH
FAKTU
N&"a, @
&a'#a a
B%&!a&'a /&$
)%&a'+
5A!a, : B&$
5S%)%,ah ((a"*a' :
Ha$
B%&!a&'a "%&ah
/a)a
1> D%)*
1 : 1 +,$*sa
(a' a&
B%&!a&'a /&$
)%&a'+
5A!a, : B&$
5S%)%,ah ((a"*a' :Ha$
B%&!a&'a "%&ah
/a)a
17 D%)*
1:2 +,$*sa
(a' a&
B%&!a&'a /&$
a+a* +%,a#
5A!a, : B&$
5S%)%,ah ((a"*a' :
Ha$
B%&!a&'a "%&ah
/a)a
2 D%)*
1: 4 +,$*sa B%&!a&'a /&$ 5A!a, : B&$ B%&!a&'a "%&ah 4 D%)*
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(a' a& a+a* +%,a# 5S%)%,ah ((a"*a' :
Ha$
/a)a
LAMPIRAN GAMBAR DATA HASIL PENGAMATAN
P%'a"/aha' +,$*sa
B%'%(-) (a' #%'a"/aha''.a
A$a(%s)
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La&$)a' %h,'+ #%'a"/aha' (a' has,'.a
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PEMBAHASAN
P%&%a*s B%'%(-) /%&$#a ,a&$)a' .a'+ "%'+a'($'+ *$#& s$,a) 'a)&$"
*a&/'a) (a' 'a)&$" s)&a)3 U (,a*$*a' (%'+a' "%'a"/ah*a' &%a+%') B%'%(-)
*%(a,a" ,a&$)a' *a&/h(&a) (a' (,a*$*a' #%"a'asa'3 S%)%,ah (,a*$*a' #%"a'asa'
)%&a( #%&$/aha' #a(a /%/%&a#a ,a&$)a' .a)$ G,$*sa &$*)sa (a' ,a*)sa3
P%&$/aha' ' /%&$#a )%&/%')$*'.a %'(a#a' "%&ah /a)a #a(a ,a&$)a' )%&s%/$)3 P&s%s #%'('+'a' *%)+a ,a&$)a' ' )(a* "%"#%'+a&$h #%&$/aha' #a(a %'(a#a'3
S%(a'+*a' ,a&$)a' .a'+ ,a' /%&!a&'a /&$ .a'+ "%&$#a*a' !a&'a as, (a& &%a+%'3
Ga,a*)sa s%'(& "%'+a,a" s%(*) #%&$/aha' s%)%,ah (#a'as*a' .a)$ )%&(a#a)
!a&'a "%&ah #a(a /a+a' #%&"$*aa' ,a&$)a' (a' s%)%,ah (('+'*a' !a&'a '
"%'+h,a'+3 Ga,a*)sa "%&$#a*a' sa,ah sa)$ *a&/h(&a) .a'+ "%'+a'($'+ +$+$s
a,(%h( (a,a" #%'+$a' )%&(a#a) +a,a*)sa )(a* )%&a( #%&$/aha' .a'+ (ha&a#*a'
s%#%&) +,$*sa &$*)sa (a' ,a*)sa3 Ha, ' "$'+*' )a/$'+ )%,ah )%&*')a"'as
,%h s%'.a!a ,a' s%h'++a )(a* "%'+has,*a' %'(a#a' s%#%&) ha,'.a *a&/h(&a)
,a' .a'+ "%'+a'($'+ +$+$s *%)' (a' a,(%h(3
B%&(asa&*a' has, #%'+a"a)a' (a)as /ah!a (a& /%&/a+a "a-a" %'s ,a&$)a'
*a&/h(&a) s%)%,ah (&%a*s*a' (%'+a' ,a&$)a' B%'%(-) )%&'.a)a &%a*s'.a )(a*
)%&a( s%-a&a /%&sa"aa'3 Ha, ' (s%/a/*a' )%&+a')$'+ a)as *'s%')&as *a&/h(&a)
.a'+ (#%&*sa /a* (sa*a&(a a)a$ "'sa*a&(a3 Pa(a (asa&'.a (a& /%&/a+a
"a-a" %'s ,a&$)a' *a&/h(&a) .a'+ ($ )%&'.a)a .a'+ /%&%a*s #s) (%'+a'
B%'%(-) ha'.a +,'+a' (sa*a&(a (a' "'sa*a&(a s%(a'+*a' ,a&$)a'
#,sa*a&(a s%#%&) a",$" )(a* (a#a) /%&%a*s (%'+a' B%'%(-)3 E'(a#a'5%'(a#a'
.a'+ (has,*a' #a(a #%&-/aa' ' a(a,ah "%&ah3 K$''+ )%&+a')$'+ a)as *$a)'.a
,a&$)a' +$,a3 B%'%(-) $+a /%&sa) /asa ,%"ah *a&%'a a(a'.a 'a)&$" *a&/'a) (a'
'a)&$" s)&a)3
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Pa(a $ %h,'+ "%'++$'a*a' ,a&$)a' %h,'+ .a'+ )%&/$a) (a& ,a&$)a'
)%"/a+a s$,a) (%'+a' 'a)&$"5*a,$" )a&)a) (a' 'a)&$" h(&*s(a3 U %h,'+s
(+$'a*a' $')$* "%'+%)ah$ a(a'.a +$,a #%&%($*s (a,a" sa"#%,3 Da& 4 sa"#%,
()%)%s s%/a'.a* 10 )%)%s ,a&$)a' %h,'+ A (a' B ;1:1< .a'+ )%,ah (-a"#$&*a'
s%/%,$"'.a3 La,$ (#a'as*a' (a,a" !a)%&/a)h s%,a"a 20 (%)* (a' (a"a) sa"#a
"$,a /%&$/ah !a&'a (a' )%&/%')$* %'(a#a'3 P%"a'asa' (,a*$*a' $')$*
"%"#%&-%#a) &%a*s3 U %h,'+s (+$'a*a' $')$* "%'+$ +,$*sa3 Da& *%%"#a)
sa"#%, )%&/%')$* %'(a#a' /%&!a&'a "%&ah )$a@ "%&ah /a)a33 La&$)a' %h,'+s (a#a)
"%'+*s(as +,$*sa .a'+ "%'+a'($'+ +$+$s a,(sa .a'+ "%",* +$+$s
a,(%h(a3 E'(a#a' .a'+ )%&/%')$* "%&$#a*a' U2O *a&%'a ,a&$)a' %h,'+s A (a'
B (a#a) "%'+has,*a' ' U2 s%/a+a #%'+*s(as sa"#%, .a'+ "%'+a'($'+a,(%h(3
P%'+%'-%&a' +,$*sa .a'+ )%&a( s%)%,ah sa"#%, (a' &%a+%' (#a'as*a'
s%,a"a 20 (%)* (a' ()a"/ah*a' (%'+a' NaOH has,'.a &%,a) (a' )(a* /%&/%(a
a$h (%'+a' *%,"#* *%,"#* ,a''.a3 a&as !a*)$ ' )%&a( *a&%'a S%"a*'
/a'.a* +,$*sa (a' "a*' (*) a& &%a*s a*a' s%"a*' -%#a)3
P%'a"/aha' NaOH ()$$*a' s$#a.a +,$*sa /%&%a*s s#%s* (%'+a' /asa
*a&%'a ,a&$)a' /%'%(-) a)a$ %h,'+ /%,$" -$*$# "a"#$ $')$* "%"/%&*a' &%a*s
.a'+ s#%s* a')a&a +$,a (%'+a' s /asa3S%,a' )$ ' $2 ha'.a (a#a) (%'(a#*a'
s%/a+a $2O ha'.a *a (a,a" s$asa'a /asa s%h'++a (a#a) "%"/%&*a' !a&'a .a'+
*has3
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KESIMPULAN
13 K)a "%'++$'a*a' &%a+%' /%'%(-) (a' &%a+%' %h,'+ s%/a+a '(*a)&
$')$* "%'(%)%*s +$,a
23 P%&/a'('+a' G,$*sa (a' a& .a'+ (+$'a*a' a(a,ah '&"a, a)a$ )a'#a a&
1:1 1:2 1:4
>3 P%"a'asa' (+$'a*a' $')$* "%"#%&-%#a) &%a*s a')a&a &%a+%' (a' s%'.a!a
43 P%'a"/aha' NaOH ()$$*a' s$#a.a +,$*sa /%&%a*s s#%s* (%'+a' /asa
*a&%'a ,a&$)a' /%'%(-) a)a$ %h,'+ /%,$" -$*$# "a"#$ $')$* "%"/%&*a'&%a*s .a'+ s#%s* a')a&a +$,a (%'+a' s /asa
3 a&as !a*)$ &%a*s #%&$/aha' !a&'a )%&a( *a&%'a S%"a*' /a'.a* +,$*sa
(a' "a*' (*) a& &%a*s a*a' s%"a*' -%#a)3
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DATAR PUSTAKA
13 %ss%'(%' Ra,#h J3a'( Ja' S3 173 O&+a'- h%"s)&. >&(
23 %(3 B%,"a') a,&'a3 I&a!a' A'!a&3 20073 Ka&/h(&a)3 P&)' S#&)
S-%'-% a'( P%&&"a'-% La/3 III 1: 154
>3 Ha'('3 Ka&/h(&a) Pa(a U K$a,)a)3 Ba'($'+3 2003 Da*s%s )a'++a, 6
Ma&%) 201> #$*$, 1:00
4. Martoharsono, Soeharsono. 1975. Biokimia. Gadjah Mada University Press. :
Yoyakarta
5. Poedjiadi, !nna. 1994. "asar#"asar Biokimia. U$#Pres : %akarta